AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040021.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040021.01.T01 MTR_7g085180 96.053 152 6 0 6 157 125 276 1.81e-103 304
MsG0780040021.01.T01 MTR_7g085150 80.519 154 30 0 2 155 142 295 8.39e-88 263
MsG0780040021.01.T01 MTR_7g085150 80.519 154 30 0 2 155 142 295 2.14e-87 263
MsG0780040021.01.T01 MTR_7g085120 79.221 154 32 0 2 155 121 274 1.70e-86 260
MsG0780040021.01.T01 MTR_7g085200 70.000 150 45 0 6 155 124 273 1.69e-74 229
MsG0780040021.01.T01 MTR_7g085200 69.079 152 45 1 6 155 124 275 1.28e-72 225
MsG0780040021.01.T01 MTR_7g085090 57.616 151 64 0 6 156 148 298 5.91e-63 200
MsG0780040021.01.T01 MTR_6g033000 56.291 151 66 0 6 156 161 311 3.97e-62 198
MsG0780040021.01.T01 MTR_4g036220 67.692 130 28 1 26 155 136 251 7.55e-57 183
MsG0780040021.01.T01 MTR_5g040710 31.579 171 90 6 2 157 154 312 3.19e-18 80.9
MsG0780040021.01.T01 MTR_8g072360 35.632 87 55 1 2 87 199 285 4.88e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040021.01.T01 AT5G18280 55.333 150 67 0 6 155 151 300 2.14e-58 188
MsG0780040021.01.T01 AT3G04080 53.333 150 70 0 6 155 150 299 2.91e-54 177
MsG0780040021.01.T01 AT1G14240 33.333 162 86 4 2 153 148 297 3.12e-21 89.4
MsG0780040021.01.T01 AT1G14240 33.333 162 86 4 2 153 148 297 3.12e-21 89.4
MsG0780040021.01.T01 AT1G14240 33.333 162 86 4 2 153 148 297 3.12e-21 89.4
MsG0780040021.01.T01 AT1G14230 31.481 162 90 3 2 153 159 309 1.24e-20 87.8
MsG0780040021.01.T01 AT1G14250 31.902 163 88 4 2 153 149 299 7.95e-20 85.5
MsG0780040021.01.T01 AT2G02970 30.539 167 91 4 2 154 165 320 1.79e-18 81.6
MsG0780040021.01.T01 AT1G14240 32.099 162 84 5 2 153 148 293 1.01e-17 79.3

Find 35 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAACTATGTATTGGGGAATT+TGG 0.093358 7:-72118832 MsG0780040021.01.T01:CDS
AGCACAAGCTTCTTAATATA+TGG 0.233885 7:+72118690 None:intergenic
AACTATGTATTGGGGAATTT+GGG 0.257906 7:-72118831 MsG0780040021.01.T01:CDS
CCCACATATAAGAACCTTCT+TGG 0.273411 7:+72119036 None:intergenic
GTGACAGTTAACTATGTATT+GGG 0.320917 7:-72118840 MsG0780040021.01.T01:CDS
TCTTGTAGTTACTTGCGTTT+TGG 0.327536 7:-72118515 MsG0780040021.01.T01:intron
AATATGATCTCTATGTTCAT+AGG 0.360539 7:-72118656 MsG0780040021.01.T01:intron
GATGCAGTTTCTATTATTGA+TGG 0.366866 7:-72119062 MsG0780040021.01.T01:CDS
TCCTCTCCATCAGCAACTTT+AGG 0.389941 7:+72118714 None:intergenic
GGTGACAGTTAACTATGTAT+TGG 0.433794 7:-72118841 MsG0780040021.01.T01:CDS
CCACATATAAGAACCTTCTT+GGG 0.448076 7:+72119037 None:intergenic
CCCAAGAAGGTTCTTATATG+TGG 0.452789 7:-72119037 MsG0780040021.01.T01:CDS
ATTATTGATGGAACCCAAGA+AGG 0.464764 7:-72119050 MsG0780040021.01.T01:CDS
TGCAGGCAACCGCAGGTTTG+AGG 0.484016 7:-72119274 MsG0780040021.01.T01:CDS
TGCTGAAAAGATATTGCAAT+CGG 0.497903 7:-72119234 MsG0780040021.01.T01:intron
TAAATATTACCATTAAATCC+AGG 0.499439 7:+72118428 None:intergenic
TATTAAGAAGCTTGTGCTCA+AGG 0.501262 7:-72118685 MsG0780040021.01.T01:CDS
TGCATCACGTTGAACGTTGA+GGG 0.516798 7:+72119079 None:intergenic
AATGCTCCTAAAGTTGCTGA+TGG 0.518455 7:-72118720 MsG0780040021.01.T01:CDS
CAAAGAAGCAACTCGAGCAC+AGG 0.521520 7:-72118492 MsG0780040021.01.T01:CDS
GGAGTAATTGACCTTGGAGG+TGG 0.525763 7:-72118786 MsG0780040021.01.T01:CDS
AATTTATATGATAATAATGC+AGG 0.531034 7:-72119291 None:intergenic
TTAAATCCAGGTAAAATGCA+AGG 0.532782 7:+72118440 None:intergenic
ATAATAATGCAGGCAACCGC+AGG 0.543205 7:-72119281 MsG0780040021.01.T01:CDS
ATTAAGAAGCTTGTGCTCAA+GGG 0.561023 7:-72118684 MsG0780040021.01.T01:CDS
CTGCATCACGTTGAACGTTG+AGG 0.583648 7:+72119078 None:intergenic
GTGGGAGTAATTGACCTTGG+AGG 0.593027 7:-72118789 MsG0780040021.01.T01:CDS
CCAAGAAGGTTCTTATATGT+GGG 0.599714 7:-72119036 MsG0780040021.01.T01:intron
TCCTAAAGTTGCTGATGGAG+AGG 0.601540 7:-72118715 MsG0780040021.01.T01:CDS
TCAGTGGGAGTAATTGACCT+TGG 0.602293 7:-72118792 MsG0780040021.01.T01:CDS
AAAAGCTTCACAAAATCAGT+GGG 0.611541 7:-72118807 MsG0780040021.01.T01:CDS
TAAATCCAGGTAAAATGCAA+GGG 0.612700 7:+72118441 None:intergenic
TTTGAACTGAACCACCTCCA+AGG 0.632699 7:+72118775 None:intergenic
ATTCAAAAGCCTCAAACCTG+CGG 0.651264 7:+72119265 None:intergenic
TGACAGTTAACTATGTATTG+GGG 0.677718 7:-72118839 MsG0780040021.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGTATATATTATATATGAA+TGG - Chr7:72119140-72119159 MsG0780040021.01.T01:intron 10.0%
!!! TCTTTTTTCTTTTTTATTAG+AGG - Chr7:72119090-72119109 MsG0780040021.01.T01:CDS 15.0%
!!! TTCTTTATGGAATTTTTTTT+GGG - Chr7:72118723-72118742 MsG0780040021.01.T01:CDS 15.0%
!!! TTTCTTTATGGAATTTTTTT+TGG - Chr7:72118722-72118741 MsG0780040021.01.T01:CDS 15.0%
!! TAAATATTACCATTAAATCC+AGG + Chr7:72119286-72119305 None:intergenic 20.0%
!!! CAGAATATATTGATTATATG+TGG - Chr7:72118784-72118803 MsG0780040021.01.T01:CDS 20.0%
! AATATGATCTCTATGTTCAT+AGG - Chr7:72119055-72119074 MsG0780040021.01.T01:CDS 25.0%
! ACACTTATAATGAATATTGG+GGG + Chr7:72118831-72118850 None:intergenic 25.0%
! CACACTTATAATGAATATTG+GGG + Chr7:72118832-72118851 None:intergenic 25.0%
! GCACACTTATAATGAATATT+GGG + Chr7:72118833-72118852 None:intergenic 25.0%
! GGCTAATTAATGTATATTGA+GGG - Chr7:72119161-72119180 MsG0780040021.01.T01:intron 25.0%
! TGGCTAATTAATGTATATTG+AGG - Chr7:72119160-72119179 MsG0780040021.01.T01:intron 25.0%
! TTTAGCAACTATACCTTAAT+TGG - Chr7:72118544-72118563 MsG0780040021.01.T01:intron 25.0%
AAAAAGCTTCACAAAATCAG+TGG - Chr7:72118903-72118922 MsG0780040021.01.T01:intron 30.0%
AAAAGCTTCACAAAATCAGT+GGG - Chr7:72118904-72118923 MsG0780040021.01.T01:intron 30.0%
AACTATGTATTGGGGAATTT+GGG - Chr7:72118880-72118899 MsG0780040021.01.T01:intron 30.0%
AGCACAAGCTTCTTAATATA+TGG + Chr7:72119024-72119043 None:intergenic 30.0%
CGCACACTTATAATGAATAT+TGG + Chr7:72118834-72118853 None:intergenic 30.0%
GATGCAGTTTCTATTATTGA+TGG - Chr7:72118649-72118668 MsG0780040021.01.T01:intron 30.0%
GGTAAGAAAACAAAAGCTTT+AGG + Chr7:72118580-72118599 None:intergenic 30.0%
GTGACAGTTAACTATGTATT+GGG - Chr7:72118871-72118890 MsG0780040021.01.T01:intron 30.0%
GTGCGTATGTATATCTTTAT+AGG - Chr7:72118849-72118868 MsG0780040021.01.T01:intron 30.0%
TAAATCCAGGTAAAATGCAA+GGG + Chr7:72119273-72119292 None:intergenic 30.0%
TAACTATGTATTGGGGAATT+TGG - Chr7:72118879-72118898 MsG0780040021.01.T01:intron 30.0%
TGACAGTTAACTATGTATTG+GGG - Chr7:72118872-72118891 MsG0780040021.01.T01:intron 30.0%
TGCTGAAAAGATATTGCAAT+CGG - Chr7:72118477-72118496 MsG0780040021.01.T01:CDS 30.0%
TTAAATCCAGGTAAAATGCA+AGG + Chr7:72119274-72119293 None:intergenic 30.0%
! TAGCAAATGCTGCTTAATTA+TGG + Chr7:72118506-72118525 None:intergenic 30.0%
! TGCATTTTACCTGGATTTAA+TGG - Chr7:72119274-72119293 MsG0780040021.01.T01:CDS 30.0%
! TTGTTTTCTTACCTTAGACA+AGG - Chr7:72118587-72118606 MsG0780040021.01.T01:intron 30.0%
AGGCATTTGAAAGCCAATTA+AGG + Chr7:72118560-72118579 None:intergenic 35.0%
ATTAAGAAGCTTGTGCTCAA+GGG - Chr7:72119027-72119046 MsG0780040021.01.T01:intron 35.0%
ATTATTGATGGAACCCAAGA+AGG - Chr7:72118661-72118680 MsG0780040021.01.T01:CDS 35.0%
CCAAGAAGGTTCTTATATGT+GGG - Chr7:72118675-72118694 MsG0780040021.01.T01:CDS 35.0%
CCACATATAAGAACCTTCTT+GGG + Chr7:72118677-72118696 None:intergenic 35.0%
CTGAACATATTCCTTGTCTA+AGG + Chr7:72118601-72118620 None:intergenic 35.0%
CTTATAATGAATATTGGGGG+AGG + Chr7:72118828-72118847 None:intergenic 35.0%
GGTGACAGTTAACTATGTAT+TGG - Chr7:72118870-72118889 MsG0780040021.01.T01:intron 35.0%
TATTAAGAAGCTTGTGCTCA+AGG - Chr7:72119026-72119045 MsG0780040021.01.T01:intron 35.0%
! TCTTGTAGTTACTTGCGTTT+TGG - Chr7:72119196-72119215 MsG0780040021.01.T01:intron 35.0%
!!! CAGGTTTTGAATGCAACTAA+TGG - Chr7:72119238-72119257 MsG0780040021.01.T01:CDS 35.0%
AATGCTCCTAAAGTTGCTGA+TGG - Chr7:72118991-72119010 MsG0780040021.01.T01:intron 40.0%
ATTCAAAAGCCTCAAACCTG+CGG + Chr7:72118449-72118468 None:intergenic 40.0%
CCCAAGAAGGTTCTTATATG+TGG - Chr7:72118674-72118693 MsG0780040021.01.T01:CDS 40.0%
CCCACATATAAGAACCTTCT+TGG + Chr7:72118678-72118697 None:intergenic 40.0%
!! AGGTTTGAGGCTTTTGAATG+GGG - Chr7:72118450-72118469 MsG0780040021.01.T01:CDS 40.0%
!! CAGGTTTGAGGCTTTTGAAT+GGG - Chr7:72118449-72118468 MsG0780040021.01.T01:CDS 40.0%
ATAATAATGCAGGCAACCGC+AGG - Chr7:72118430-72118449 MsG0780040021.01.T01:CDS 45.0%
TCAGTGGGAGTAATTGACCT+TGG - Chr7:72118919-72118938 MsG0780040021.01.T01:intron 45.0%
TCCTAAAGTTGCTGATGGAG+AGG - Chr7:72118996-72119015 MsG0780040021.01.T01:intron 45.0%
TCCTCTCCATCAGCAACTTT+AGG + Chr7:72119000-72119019 None:intergenic 45.0%
TGCATCACGTTGAACGTTGA+GGG + Chr7:72118635-72118654 None:intergenic 45.0%
TTTGAACTGAACCACCTCCA+AGG + Chr7:72118939-72118958 None:intergenic 45.0%
! GCTAACCCTTGCATTTTACC+TGG - Chr7:72119265-72119284 MsG0780040021.01.T01:CDS 45.0%
!! GCAGGTTTGAGGCTTTTGAA+TGG - Chr7:72118448-72118467 MsG0780040021.01.T01:CDS 45.0%
!! ATAAAATTAAAATTTCTTTA+TGG - Chr7:72118710-72118729 MsG0780040021.01.T01:CDS 5.0%
!!! ATAATGTTAATTTTTTTTTT+TGG - Chr7:72118761-72118780 MsG0780040021.01.T01:CDS 5.0%
CAAAGAAGCAACTCGAGCAC+AGG - Chr7:72119219-72119238 MsG0780040021.01.T01:intron 50.0%
CTGCATCACGTTGAACGTTG+AGG + Chr7:72118636-72118655 None:intergenic 50.0%
GGAGTAATTGACCTTGGAGG+TGG - Chr7:72118925-72118944 MsG0780040021.01.T01:intron 50.0%
GTGGGAGTAATTGACCTTGG+AGG - Chr7:72118922-72118941 MsG0780040021.01.T01:intron 50.0%
TGCAGGCAACCGCAGGTTTG+AGG - Chr7:72118437-72118456 MsG0780040021.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 72118427 72119306 72118427 ID=MsG0780040021.01;Name=MsG0780040021.01
Chr7 mRNA 72118427 72119306 72118427 ID=MsG0780040021.01.T01;Parent=MsG0780040021.01;Name=MsG0780040021.01.T01;_AED=0.49;_eAED=0.50;_QI=0|0|0|1|1|1|4|0|157
Chr7 exon 72119235 72119306 72119235 ID=MsG0780040021.01.T01:exon:34936;Parent=MsG0780040021.01.T01
Chr7 exon 72119037 72119129 72119037 ID=MsG0780040021.01.T01:exon:34937;Parent=MsG0780040021.01.T01
Chr7 exon 72118657 72118862 72118657 ID=MsG0780040021.01.T01:exon:34938;Parent=MsG0780040021.01.T01
Chr7 exon 72118427 72118529 72118427 ID=MsG0780040021.01.T01:exon:34939;Parent=MsG0780040021.01.T01
Chr7 CDS 72119235 72119306 72119235 ID=MsG0780040021.01.T01:cds;Parent=MsG0780040021.01.T01
Chr7 CDS 72119037 72119129 72119037 ID=MsG0780040021.01.T01:cds;Parent=MsG0780040021.01.T01
Chr7 CDS 72118657 72118862 72118657 ID=MsG0780040021.01.T01:cds;Parent=MsG0780040021.01.T01
Chr7 CDS 72118427 72118529 72118427 ID=MsG0780040021.01.T01:cds;Parent=MsG0780040021.01.T01
Gene Sequence

>MsG0780040021.01.T01

ATGATAATAATGCAGGCAACCGCAGGTTTGAGGCTTTTGAATGGGGATGCTGCTGAAAAGATATTGCAATCGACAAGGAATATGTTCAGCAACAGAAGTACCCTCAACGTTCAACGTGATGCAGTTTCTATTATTGATGGAACCCAAGAAGGTTCTTATATGTGGGTGACAGTTAACTATGTATTGGGGAATTTGGGAAAAAGCTTCACAAAATCAGTGGGAGTAATTGACCTTGGAGGTGGTTCAGTTCAAATGACATATGCAGTGTCAAAGAAAACAGCAAAAAATGCTCCTAAAGTTGCTGATGGAGAGGATCCATATATTAAGAAGCTTGTGCTCAAGGGAAAGCAATATGATCTCTATGTTCATAGTTACTTGCGTTTTGGCAAAGAAGCAACTCGAGCACAGGTTTTGAATGCAACTAATGGATCTGCTAACCCTTGCATTTTACCTGGATTTAATGGTAATATTTAA

Protein sequence

>MsG0780040021.01.T01

MIIMQATAGLRLLNGDAAEKILQSTRNMFSNRSTLNVQRDAVSIIDGTQEGSYMWVTVNYVLGNLGKSFTKSVGVIDLGGGSVQMTYAVSKKTAKNAPKVADGEDPYIKKLVLKGKQYDLYVHSYLRFGKEATRAQVLNATNGSANPCILPGFNGNI*