AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040022.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040022.01.T01 MTR_7g085180 94.495 109 6 0 55 163 16 124 2.01e-69 217
MsG0780040022.01.T01 MTR_7g085200 79.032 124 25 1 40 163 1 123 7.16e-64 202
MsG0780040022.01.T01 MTR_7g085200 79.032 124 25 1 40 163 1 123 8.18e-64 202
MsG0780040022.01.T01 MTR_7g085120 80.645 124 24 0 40 163 1 124 2.20e-59 191
MsG0780040022.01.T01 MTR_4g036220 73.228 127 32 2 38 163 9 134 2.44e-58 187
MsG0780040022.01.T01 MTR_7g085150 72.441 127 33 2 38 163 20 145 4.68e-58 187
MsG0780040022.01.T01 MTR_7g085150 72.441 127 33 2 38 163 20 145 1.12e-57 187
MsG0780040022.01.T01 MTR_7g085090 54.237 118 54 0 46 163 30 147 1.81e-39 139
MsG0780040022.01.T01 MTR_6g033000 50.000 110 55 0 54 163 51 160 3.61e-32 119
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040022.01.T01 AT3G04080 46.789 109 58 0 55 163 41 149 1.17e-26 104
MsG0780040022.01.T01 AT5G18280 54.839 93 42 0 71 163 58 150 1.96e-26 103
MsG0780040022.01.T01 AT1G14240 31.496 127 80 3 38 162 29 150 5.29e-12 63.2
MsG0780040022.01.T01 AT1G14240 31.496 127 80 3 38 162 29 150 5.29e-12 63.2
MsG0780040022.01.T01 AT1G14240 31.496 127 80 3 38 162 29 150 5.29e-12 63.2
MsG0780040022.01.T01 AT1G14240 31.496 127 80 3 38 162 29 150 5.43e-12 63.2
MsG0780040022.01.T01 AT1G14250 43.662 71 38 1 84 152 71 141 2.87e-11 61.2

Find 41 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCAAGCCTAAGGGGTGTTT+TGG 0.190497 7:+72121187 None:intergenic
TCCTCCACCTCTTCAATCTT+TGG 0.221845 7:+72122283 None:intergenic
AAAATCTCTCATTCCACTTT+TGG 0.222729 7:-72121245 MsG0780040022.01.T01:CDS
ATCACTTCCTCCCAATATTT+AGG 0.236902 7:-72121607 MsG0780040022.01.T01:CDS
GTAGGTTGTTTCCTAAATAT+TGG 0.312837 7:+72121596 None:intergenic
TAGGTTGTTTCCTAAATATT+GGG 0.321694 7:+72121597 None:intergenic
AAGTGGCAATGAGTGTAATT+AGG 0.332469 7:+72121653 None:intergenic
CAATTAAAACCGTTAGATCT+TGG 0.355506 7:-72122328 MsG0780040022.01.T01:CDS
GATCTAAGTTCTGATCAAAA+TGG 0.383146 7:+72121479 None:intergenic
CCAAAACACCCCTTAGGCTT+GGG 0.389215 7:-72121187 MsG0780040022.01.T01:CDS
AAACCAGGTTTGAGTGCATA+TGG 0.392234 7:-72121289 MsG0780040022.01.T01:CDS
TATGACAGCGTAAGAGGTTA+AGG 0.393565 7:+72121540 None:intergenic
ATCAAATATGACAGCGTAAG+AGG 0.408728 7:+72121534 None:intergenic
TCCATGTTCTTAATTTGTGT+TGG 0.446135 7:+72121679 None:intergenic
CCCAAAACACCCCTTAGGCT+TGG 0.470228 7:-72121188 MsG0780040022.01.T01:CDS
TATTGGGAGGAAGTGATTGC+AGG 0.484200 7:+72121613 None:intergenic
GAAAACTCACCCCAAGCCTA+AGG 0.486209 7:+72121177 None:intergenic
TACGCTGTCATATTTGATGC+TGG 0.490658 7:-72121529 MsG0780040022.01.T01:CDS
TCTTCTGCTTCCTCCAAAAG+TGG 0.496021 7:+72121232 None:intergenic
AACCAGGTTTGAGTGCATAT+GGG 0.507093 7:-72121288 MsG0780040022.01.T01:CDS
TCCCCATATGCACTCAAACC+TGG 0.510774 7:+72121286 None:intergenic
AAATCTTTCGATTAGTGAGT+AGG 0.512314 7:+72121578 None:intergenic
ATATTTGATGCTGGTAGCAC+TGG 0.520451 7:-72121520 MsG0780040022.01.T01:CDS
ACATAGTGGATTGTGAATCA+TGG 0.525218 7:-72121703 MsG0780040022.01.T01:intron
AAAACTCACCCCAAGCCTAA+GGG 0.532118 7:+72121178 None:intergenic
CTTGGCTTAAATAGAAAACA+TGG 0.543407 7:-72122310 MsG0780040022.01.T01:CDS
CTATTTAAGCCAAGATCTAA+CGG 0.556224 7:+72122319 None:intergenic
TGATATTGAGTTTGTTGACA+AGG 0.580220 7:-72121440 MsG0780040022.01.T01:intron
ACCAGGTTTGAGTGCATATG+GGG 0.591814 7:-72121287 MsG0780040022.01.T01:CDS
TTTGGAGGAAGCAGAAGATG+TGG 0.592449 7:-72121227 MsG0780040022.01.T01:CDS
TCTGATCAAAATGGTAAACA+TGG 0.600597 7:+72121488 None:intergenic
GCTTAAATAGAAAACATGGA+TGG 0.613259 7:-72122306 MsG0780040022.01.T01:CDS
CAAAACACCCCTTAGGCTTG+GGG 0.614369 7:-72121186 MsG0780040022.01.T01:CDS
AACTTAGATCTACTTCACAT+TGG 0.619361 7:-72121466 MsG0780040022.01.T01:CDS
AGCAGAAGATGTGGTTCCTG+AGG 0.623737 7:-72121218 MsG0780040022.01.T01:CDS
GTTGTTTCCTAAATATTGGG+AGG 0.625266 7:+72121600 None:intergenic
ACCAACACAAATTAAGAACA+TGG 0.628977 7:-72121680 MsG0780040022.01.T01:CDS
CTGCACCCAAAACACCCCTT+AGG 0.632559 7:-72121193 MsG0780040022.01.T01:CDS
AAACTCACCCCAAGCCTAAG+GGG 0.632954 7:+72121179 None:intergenic
TGTGTGATGTAGATCAAACC+AGG 0.641736 7:-72121304 MsG0780040022.01.T01:intron
TGGATGGCCAAAGATTGAAG+AGG 0.715390 7:-72122290 MsG0780040022.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATGTAATTTTTTTTAACAA+AGG + Chr7:72122141-72122160 None:intergenic 10.0%
!! TTATTATCAACAGTAAAATT+TGG + Chr7:72121416-72121435 None:intergenic 15.0%
!! AAGATAGACTCATTATAATA+TGG + Chr7:72121714-72121733 None:intergenic 20.0%
!! TAAACAAGTTAAATCATTGA+GGG + Chr7:72121610-72121629 None:intergenic 20.0%
!! TAAATTATGTTATAATTCGC+TGG + Chr7:72121739-72121758 None:intergenic 20.0%
!! TTAAACAAGTTAAATCATTG+AGG + Chr7:72121611-72121630 None:intergenic 20.0%
! ATAATATGGACATATGGTTA+TGG + Chr7:72121700-72121719 None:intergenic 25.0%
! CATTAACAAGAGTAAAAAAG+TGG + Chr7:72121899-72121918 None:intergenic 25.0%
! CTCATTATAATATGGACATA+TGG + Chr7:72121706-72121725 None:intergenic 25.0%
! TAATATGGACATATGGTTAT+GGG + Chr7:72121699-72121718 None:intergenic 25.0%
!! CAAGGTTAGATTTTCAATAT+TGG - Chr7:72122110-72122129 MsG0780040022.01.T01:intron 25.0%
!! TAGGTTGTTTCCTAAATATT+GGG + Chr7:72121938-72121957 None:intergenic 25.0%
!!! ACAGTAAAATTTGGTGTTTA+AGG + Chr7:72121407-72121426 None:intergenic 25.0%
!!! CAGTAAAATTTGGTGTTTAA+GGG + Chr7:72121406-72121425 None:intergenic 25.0%
!!! TATTTCATCTTCACTTTTTC+CGG - Chr7:72121802-72121821 MsG0780040022.01.T01:intron 25.0%
!!! TTCTCGTGGTATAATTTTTT+GGG - Chr7:72121300-72121319 MsG0780040022.01.T01:intron 25.0%
AAATCTTTCGATTAGTGAGT+AGG + Chr7:72121957-72121976 None:intergenic 30.0%
AACTTAGATCTACTTCACAT+TGG - Chr7:72122066-72122085 MsG0780040022.01.T01:intron 30.0%
ACCAACACAAATTAAGAACA+TGG - Chr7:72121852-72121871 MsG0780040022.01.T01:intron 30.0%
CAATTAAAACCGTTAGATCT+TGG - Chr7:72121204-72121223 MsG0780040022.01.T01:CDS 30.0%
CTATTTAAGCCAAGATCTAA+CGG + Chr7:72121216-72121235 None:intergenic 30.0%
CTTGGCTTAAATAGAAAACA+TGG - Chr7:72121222-72121241 MsG0780040022.01.T01:CDS 30.0%
GCTTAAATAGAAAACATGGA+TGG - Chr7:72121226-72121245 MsG0780040022.01.T01:CDS 30.0%
TCTGATCAAAATGGTAAACA+TGG + Chr7:72122047-72122066 None:intergenic 30.0%
TTGATCAAATGAGATGTGTT+TGG + Chr7:72121512-72121531 None:intergenic 30.0%
! AAAATCTCTCATTCCACTTT+TGG - Chr7:72122287-72122306 MsG0780040022.01.T01:intron 30.0%
! GATCTAAGTTCTGATCAAAA+TGG + Chr7:72122056-72122075 None:intergenic 30.0%
! GTAGGTTGTTTCCTAAATAT+TGG + Chr7:72121939-72121958 None:intergenic 30.0%
!! TCCATGTTCTTAATTTGTGT+TGG + Chr7:72121856-72121875 None:intergenic 30.0%
!! TGATATTGAGTTTGTTGACA+AGG - Chr7:72122092-72122111 MsG0780040022.01.T01:intron 30.0%
!!! AGGTTAAGGTTTCTTGTTTT+TGG + Chr7:72121981-72122000 None:intergenic 30.0%
!!! CTTCTCGTGGTATAATTTTT+TGG - Chr7:72121299-72121318 MsG0780040022.01.T01:intron 30.0%
AAGTGGCAATGAGTGTAATT+AGG + Chr7:72121882-72121901 None:intergenic 35.0%
ATCAAATATGACAGCGTAAG+AGG + Chr7:72122001-72122020 None:intergenic 35.0%
ATCACTTCCTCCCAATATTT+AGG - Chr7:72121925-72121944 MsG0780040022.01.T01:intron 35.0%
ATGAGATGTGTTTGGTAAAC+AGG + Chr7:72121504-72121523 None:intergenic 35.0%
CACAATCCACTATGTATAAC+CGG + Chr7:72121824-72121843 None:intergenic 35.0%
GGGAAAACTATCGCAAATAT+TGG - Chr7:72121320-72121339 MsG0780040022.01.T01:intron 35.0%
! CTTTTTCCGGTTATACATAG+TGG - Chr7:72121815-72121834 MsG0780040022.01.T01:intron 35.0%
! GTTGTTTCCTAAATATTGGG+AGG + Chr7:72121935-72121954 None:intergenic 35.0%
!! ACATAGTGGATTGTGAATCA+TGG - Chr7:72121829-72121848 MsG0780040022.01.T01:intron 35.0%
AAACCAGGTTTGAGTGCATA+TGG - Chr7:72122243-72122262 MsG0780040022.01.T01:intron 40.0%
AACCAGGTTTGAGTGCATAT+GGG - Chr7:72122244-72122263 MsG0780040022.01.T01:intron 40.0%
ATATTTGATGCTGGTAGCAC+TGG - Chr7:72122012-72122031 MsG0780040022.01.T01:intron 40.0%
TACGCTGTCATATTTGATGC+TGG - Chr7:72122003-72122022 MsG0780040022.01.T01:intron 40.0%
TATGACAGCGTAAGAGGTTA+AGG + Chr7:72121995-72122014 None:intergenic 40.0%
TGTGTGATGTAGATCAAACC+AGG - Chr7:72122228-72122247 MsG0780040022.01.T01:intron 40.0%
TTGATCGAATCACTCTGACA+AGG + Chr7:72121648-72121667 None:intergenic 40.0%
! TTGGTGTTTAAGGGACTAGT+CGG + Chr7:72121397-72121416 None:intergenic 40.0%
!! AGAGATTTTGCTGCTTGTTC+AGG + Chr7:72122276-72122295 None:intergenic 40.0%
!! ATCTCTCATTCCACTTTTGG+AGG - Chr7:72122290-72122309 MsG0780040022.01.T01:intron 40.0%
AAAGATTGAAGAGGTGGAGG+AGG - Chr7:72121251-72121270 MsG0780040022.01.T01:CDS 45.0%
AAGATTGAAGAGGTGGAGGA+GGG - Chr7:72121252-72121271 MsG0780040022.01.T01:CDS 45.0%
ACCAGGTTTGAGTGCATATG+GGG - Chr7:72122245-72122264 MsG0780040022.01.T01:intron 45.0%
ATGGCCAAAGATTGAAGAGG+TGG - Chr7:72121245-72121264 MsG0780040022.01.T01:CDS 45.0%
CTAACATCGTGATCTTCTCG+TGG - Chr7:72121286-72121305 MsG0780040022.01.T01:CDS 45.0%
TATTGGGAGGAAGTGATTGC+AGG + Chr7:72121922-72121941 None:intergenic 45.0%
TCCTCCACCTCTTCAATCTT+TGG + Chr7:72121252-72121271 None:intergenic 45.0%
TCTTCTGCTTCCTCCAAAAG+TGG + Chr7:72122303-72122322 None:intergenic 45.0%
TGGATGGCCAAAGATTGAAG+AGG - Chr7:72121242-72121261 MsG0780040022.01.T01:CDS 45.0%
TTTGGAGGAAGCAGAAGATG+TGG - Chr7:72122305-72122324 MsG0780040022.01.T01:CDS 45.0%
AGATTGAAGAGGTGGAGGAG+GGG - Chr7:72121253-72121272 MsG0780040022.01.T01:CDS 50.0%
AGCAGAAGATGTGGTTCCTG+AGG - Chr7:72122314-72122333 MsG0780040022.01.T01:CDS 50.0%
CAAAACACCCCTTAGGCTTG+GGG - Chr7:72122346-72122365 MsG0780040022.01.T01:CDS 50.0%
CCAAAACACCCCTTAGGCTT+GGG - Chr7:72122345-72122364 MsG0780040022.01.T01:CDS 50.0%
GCCAAAGATTGAAGAGGTGG+AGG - Chr7:72121248-72121267 MsG0780040022.01.T01:CDS 50.0%
TCCCCATATGCACTCAAACC+TGG + Chr7:72122249-72122268 None:intergenic 50.0%
!! TGTTTTGGGTGCAGATCCTC+AGG + Chr7:72122333-72122352 None:intergenic 50.0%
!!! CCAAGCCTAAGGGGTGTTTT+GGG + Chr7:72122347-72122366 None:intergenic 50.0%
CCCAAAACACCCCTTAGGCT+TGG - Chr7:72122344-72122363 MsG0780040022.01.T01:CDS 55.0%
CTGCACCCAAAACACCCCTT+AGG - Chr7:72122339-72122358 MsG0780040022.01.T01:CDS 55.0%
!!! CCCAAGCCTAAGGGGTGTTT+TGG + Chr7:72122348-72122367 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr7 gene 72121183 72122371 72121183 ID=MsG0780040022.01;Name=MsG0780040022.01
Chr7 mRNA 72121183 72122371 72121183 ID=MsG0780040022.01.T01;Parent=MsG0780040022.01;Name=MsG0780040022.01.T01;_AED=0.49;_eAED=0.60;_QI=0|0|0|1|0.5|0.66|3|0|163
Chr7 exon 72122291 72122371 72122291 ID=MsG0780040022.01.T01:exon:34933;Parent=MsG0780040022.01.T01
Chr7 exon 72121441 72121719 72121441 ID=MsG0780040022.01.T01:exon:34934;Parent=MsG0780040022.01.T01
Chr7 exon 72121183 72121314 72121183 ID=MsG0780040022.01.T01:exon:34935;Parent=MsG0780040022.01.T01
Chr7 CDS 72122291 72122371 72122291 ID=MsG0780040022.01.T01:cds;Parent=MsG0780040022.01.T01
Chr7 CDS 72121441 72121719 72121441 ID=MsG0780040022.01.T01:cds;Parent=MsG0780040022.01.T01
Chr7 CDS 72121183 72121314 72121183 ID=MsG0780040022.01.T01:cds;Parent=MsG0780040022.01.T01
Gene Sequence

>MsG0780040022.01.T01

ATGTCTCGAAAATATCACGAACAATTAAAACCGTTAGATCTTGGCTTAAATAGAAAACATGGATGGCCAAAGATTGAAGAGTGGATTGTGAATCATGGACCAACACAAATTAAGAACATGGAGTTCCTAATTACACTCATTGCCACTTTTTTACTCTTGTTAATGCCTGCAATCACTTCCTCCCAATATTTAGGAAACAACCTACTCACTAATCGAAAGATTTTCCAAAAACAAGAAACCTTAACCTCTTACGCTGTCATATTTGATGCTGGTAGCACTGGTACTCGTGTCCATGTTTACCATTTTGATCAGAACTTAGATCTACTTCACATTGGCAATGATATTGAGTTTGTTGACAAGATCAAACCAGGTTTGAGTGCATATGGGGATAATCCTGAACAAGCAGCAAAATCTCTCATTCCACTTTTGGAGGAAGCAGAAGATGTGGTTCCTGAGGATCTGCACCCAAAACACCCCTTAGGCTTGGGGTGA

Protein sequence

>MsG0780040022.01.T01

MSRKYHEQLKPLDLGLNRKHGWPKIEEWIVNHGPTQIKNMEFLITLIATFLLLLMPAITSSQYLGNNLLTNRKIFQKQETLTSYAVIFDAGSTGTRVHVYHFDQNLDLLHIGNDIEFVDKIKPGLSAYGDNPEQAAKSLIPLLEEAEDVVPEDLHPKHPLGLG*