Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040577.01.T01 | XP_003625255.1 | 93.333 | 45 | 3 | 0 | 1 | 45 | 41 | 85 | 1.17E-21 | 90.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040577.01.T01 | Q01417 | 70.27 | 37 | 11 | 0 | 1 | 37 | 40 | 76 | 1.04E-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040577.01.T01 | G7KVD1 | 93.333 | 45 | 3 | 0 | 1 | 45 | 41 | 85 | 5.60e-22 | 90.9 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048938.01 | MsG0780040577.01 | 0.823163 | 1.605614e-53 | 1.371806e-50 |
| MsG0180000246.01 | MsG0780040577.01 | 0.828602 | 8.206309e-55 | 8.195971e-52 |
| MsG0180000784.01 | MsG0780040577.01 | 0.805851 | 1.093399e-49 | 5.846678e-47 |
| MsG0180001100.01 | MsG0780040577.01 | 0.860193 | 2.449093e-63 | 6.639339e-60 |
| MsG0180005184.01 | MsG0780040577.01 | 0.815282 | 1.001491e-51 | 6.875314e-49 |
| MsG0180005418.01 | MsG0780040577.01 | 0.808172 | 3.529430e-50 | 2.004072e-47 |
| MsG0780040577.01 | MsG0880045285.01 | 0.802747 | 4.843623e-49 | 2.391472e-46 |
| MsG0780040577.01 | MsG0880045558.01 | 0.808257 | 3.385735e-50 | 1.926781e-47 |
| MsG0780040577.01 | MsG0880047152.01 | 0.819451 | 1.153770e-52 | 8.881905e-50 |
| MsG0780040577.01 | MsG0880047451.01 | 0.809040 | 2.302453e-50 | 1.337535e-47 |
| MsG0780040577.01 | MsG0880047614.01 | 0.854004 | 1.642434e-61 | 3.616320e-58 |
| MsG0280010564.01 | MsG0780040577.01 | 0.811872 | 5.634048e-51 | 3.527672e-48 |
| MsG0280011073.01 | MsG0780040577.01 | 0.801339 | 9.436032e-49 | 4.495048e-46 |
| MsG0380013342.01 | MsG0780040577.01 | 0.804578 | 2.019704e-49 | 1.045126e-46 |
| MsG0680031469.01 | MsG0780040577.01 | 0.828130 | 1.066925e-54 | 1.051039e-51 |
| MsG0680032431.01 | MsG0780040577.01 | 0.800088 | 1.697958e-48 | 7.836298e-46 |
| MsG0680034766.01 | MsG0780040577.01 | 0.810749 | 9.873321e-51 | 6.001317e-48 |
| MsG0680034767.01 | MsG0780040577.01 | 0.838716 | 2.437674e-57 | 3.293037e-54 |
| MsG0680035307.01 | MsG0780040577.01 | 0.820143 | 8.016315e-53 | 6.291734e-50 |
| MsG0480018848.01 | MsG0780040577.01 | 0.827739 | 1.324634e-54 | 1.289987e-51 |
| MsG0480019285.01 | MsG0780040577.01 | 0.801789 | 7.627644e-49 | 3.675179e-46 |
| MsG0480021468.01 | MsG0780040577.01 | 0.818976 | 1.479624e-52 | 1.124294e-49 |
| MsG0480022647.01 | MsG0780040577.01 | 0.806816 | 6.845420e-50 | 3.751790e-47 |
| MsG0580024873.01 | MsG0780040577.01 | 0.807300 | 5.406620e-50 | 3.000712e-47 |
| MsG0580025906.01 | MsG0780040577.01 | 0.801142 | 1.035224e-48 | 4.907242e-46 |
| MsG0380016194.01 | MsG0780040577.01 | 0.811964 | 5.379426e-51 | 3.376663e-48 |
| MsG0580029312.01 | MsG0780040577.01 | 0.818553 | 1.846218e-52 | 1.386359e-49 |
| MsG0580029912.01 | MsG0780040577.01 | 0.803843 | 2.872927e-49 | 1.458873e-46 |
| MsG0280008419.01 | MsG0780040577.01 | 0.827447 | 1.556634e-54 | 1.503265e-51 |
| MsG0280009757.01 | MsG0780040577.01 | 0.804353 | 2.250015e-49 | 1.157595e-46 |
| MsG0780037662.01 | MsG0780040577.01 | 0.826337 | 2.867776e-54 | 2.681770e-51 |
| MsG0780039975.01 | MsG0780040577.01 | 0.801495 | 8.763633e-49 | 4.191325e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040577.01.T01 | MTR_7g093140 | 93.333 | 45 | 3 | 0 | 1 | 45 | 41 | 85 | 1.42e-25 | 90.9 |
| MsG0780040577.01.T01 | MTR_7g093160 | 86.667 | 45 | 6 | 0 | 1 | 45 | 41 | 85 | 2.29e-22 | 82.8 |
| MsG0780040577.01.T01 | MTR_7g093170 | 75.000 | 36 | 9 | 0 | 1 | 36 | 40 | 75 | 2.61e-12 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAACCCTTTCTTCTTTCTTT+TGG | 0.103265 | 7:+79440102 | None:intergenic |
| AGCCACCACCGGGCACCAAT+TGG | 0.345146 | 7:-79440033 | MsG0780040577.01.T01:CDS |
| GCCACCACCGGGCACCAATT+GGG | 0.352332 | 7:-79440032 | MsG0780040577.01.T01:CDS |
| GCTCAAACGAGGAGTCAGTT+TGG | 0.390088 | 7:-79439971 | MsG0780040577.01.T01:CDS |
| CTCAAACGAGGAGTCAGTTT+GGG | 0.391807 | 7:-79439970 | MsG0780040577.01.T01:CDS |
| GGTGTCCACCCTGCCCCAAT+TGG | 0.432127 | 7:+79440018 | None:intergenic |
| GACACCACACACACGGGAAC+TGG | 0.439075 | 7:-79440001 | MsG0780040577.01.T01:CDS |
| CCCCAATTGGTGCCCGGTGG+TGG | 0.440868 | 7:+79440031 | None:intergenic |
| CAGGCTGAGCTTGATAAAGA+TGG | 0.466808 | 7:-79440077 | MsG0780040577.01.T01:CDS |
| CACCGGGCACCAATTGGGGC+AGG | 0.475121 | 7:-79440027 | MsG0780040577.01.T01:CDS |
| ACCACACACACGGGAACTGG+TGG | 0.487198 | 7:-79439998 | MsG0780040577.01.T01:CDS |
| CAGGGTGGACACCACACACA+CGG | 0.494311 | 7:-79440008 | MsG0780040577.01.T01:CDS |
| ACCCTGCCCCAATTGGTGCC+CGG | 0.496564 | 7:+79440025 | None:intergenic |
| AGGCTGAGCTTGATAAAGAT+GGG | 0.512672 | 7:-79440076 | MsG0780040577.01.T01:CDS |
| ATCACATGATCCCTTGCAAA+AGG | 0.516543 | 7:-79440135 | MsG0780040577.01.T01:CDS |
| CATCTTTATCAAGCTCAGCC+TGG | 0.527387 | 7:+79440078 | None:intergenic |
| AACCCAAAAGAAAGAAGAAA+GGG | 0.533989 | 7:-79440105 | MsG0780040577.01.T01:CDS |
| CACAACGCTGCAGCCACCAC+CGG | 0.534674 | 7:-79440044 | MsG0780040577.01.T01:CDS |
| GGGCTGTGAGTCACATGGTA+TGG | 0.542076 | 7:-79439950 | MsG0780040577.01.T01:CDS |
| CAACCCAAAAGAAAGAAGAA+AGG | 0.548482 | 7:-79440106 | MsG0780040577.01.T01:CDS |
| CCACCACCGGGCACCAATTG+GGG | 0.551603 | 7:-79440031 | MsG0780040577.01.T01:CDS |
| CACACACACGGGAACTGGTG+GGG | 0.561517 | 7:-79439996 | MsG0780040577.01.T01:CDS |
| GAAAGAAGAAAGGGTTAACC+AGG | 0.561684 | 7:-79440096 | MsG0780040577.01.T01:CDS |
| TGATCCCTTGCAAAAGGAGA+TGG | 0.570165 | 7:-79440129 | MsG0780040577.01.T01:CDS |
| ACAACGCTGCAGCCACCACC+GGG | 0.572456 | 7:-79440043 | MsG0780040577.01.T01:CDS |
| AGGGTGGACACCACACACAC+GGG | 0.580947 | 7:-79440007 | MsG0780040577.01.T01:CDS |
| CCACACACACGGGAACTGGT+GGG | 0.596696 | 7:-79439997 | MsG0780040577.01.T01:CDS |
| CCCACCAGTTCCCGTGTGTG+TGG | 0.613221 | 7:+79439997 | None:intergenic |
| CTGGTGGGGCTGCTCAAACG+AGG | 0.620605 | 7:-79439982 | MsG0780040577.01.T01:CDS |
| GTCACATGGTATGGAACTAG+TGG | 0.632095 | 7:-79439941 | None:intergenic |
| ACCGGGCACCAATTGGGGCA+GGG | 0.632230 | 7:-79440026 | MsG0780040577.01.T01:CDS |
| GGCTGAGCTTGATAAAGATG+GGG | 0.657041 | 7:-79440075 | MsG0780040577.01.T01:CDS |
| GGGCACCAATTGGGGCAGGG+TGG | 0.662871 | 7:-79440023 | MsG0780040577.01.T01:CDS |
| AGTTTGGGCTGTGAGTCACA+TGG | 0.670533 | 7:-79439955 | MsG0780040577.01.T01:CDS |
| CTGCCCCAATTGGTGCCCGG+TGG | 0.696266 | 7:+79440028 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACCCAAAAGAAAGAAGAAA+GGG | - | Chr7:79439979-79439998 | MsG0780040577.01.T01:CDS | 30.0% | |
| ! | AACCCTTTCTTCTTTCTTTT+GGG | + | Chr7:79439984-79440003 | None:intergenic | 30.0% |
| ! | TAACCCTTTCTTCTTTCTTT+TGG | + | Chr7:79439985-79440004 | None:intergenic | 30.0% |
| CAACCCAAAAGAAAGAAGAA+AGG | - | Chr7:79439978-79439997 | MsG0780040577.01.T01:CDS | 35.0% | |
| AGGCTGAGCTTGATAAAGAT+GGG | - | Chr7:79440008-79440027 | MsG0780040577.01.T01:CDS | 40.0% | |
| ATCACATGATCCCTTGCAAA+AGG | - | Chr7:79439949-79439968 | MsG0780040577.01.T01:CDS | 40.0% | |
| GAAAGAAGAAAGGGTTAACC+AGG | - | Chr7:79439988-79440007 | MsG0780040577.01.T01:CDS | 40.0% | |
| CAGGCTGAGCTTGATAAAGA+TGG | - | Chr7:79440007-79440026 | MsG0780040577.01.T01:CDS | 45.0% | |
| CATCTTTATCAAGCTCAGCC+TGG | + | Chr7:79440009-79440028 | None:intergenic | 45.0% | |
| CTCAAACGAGGAGTCAGTTT+GGG | - | Chr7:79440114-79440133 | MsG0780040577.01.T01:CDS | 45.0% | |
| GGCTGAGCTTGATAAAGATG+GGG | - | Chr7:79440009-79440028 | MsG0780040577.01.T01:CDS | 45.0% | |
| TGATCCCTTGCAAAAGGAGA+TGG | - | Chr7:79439955-79439974 | MsG0780040577.01.T01:CDS | 45.0% | |
| ! | GTTGCCATCTCCTTTTGCAA+GGG | + | Chr7:79439962-79439981 | None:intergenic | 45.0% |
| AGTTTGGGCTGTGAGTCACA+TGG | - | Chr7:79440129-79440148 | MsG0780040577.01.T01:CDS | 50.0% | |
| GCTCAAACGAGGAGTCAGTT+TGG | - | Chr7:79440113-79440132 | MsG0780040577.01.T01:CDS | 50.0% | |
| ! | GGTTGCCATCTCCTTTTGCA+AGG | + | Chr7:79439963-79439982 | None:intergenic | 50.0% |
| GGGCTGTGAGTCACATGGTA+TGG | - | Chr7:79440134-79440153 | MsG0780040577.01.T01:CDS | 55.0% | |
| ACCACACACACGGGAACTGG+TGG | - | Chr7:79440086-79440105 | MsG0780040577.01.T01:CDS | 60.0% | |
| AGGGTGGACACCACACACAC+GGG | - | Chr7:79440077-79440096 | MsG0780040577.01.T01:CDS | 60.0% | |
| CACACACACGGGAACTGGTG+GGG | - | Chr7:79440088-79440107 | MsG0780040577.01.T01:CDS | 60.0% | |
| CAGGGTGGACACCACACACA+CGG | - | Chr7:79440076-79440095 | MsG0780040577.01.T01:CDS | 60.0% | |
| CCACACACACGGGAACTGGT+GGG | - | Chr7:79440087-79440106 | MsG0780040577.01.T01:CDS | 60.0% | |
| GACACCACACACACGGGAAC+TGG | - | Chr7:79440083-79440102 | MsG0780040577.01.T01:CDS | 60.0% | |
| ACAACGCTGCAGCCACCACC+GGG | - | Chr7:79440041-79440060 | MsG0780040577.01.T01:CDS | 65.0% | |
| ACCGGGCACCAATTGGGGCA+GGG | - | Chr7:79440058-79440077 | MsG0780040577.01.T01:CDS | 65.0% | |
| AGCCACCACCGGGCACCAAT+TGG | - | Chr7:79440051-79440070 | MsG0780040577.01.T01:CDS | 65.0% | |
| CACAACGCTGCAGCCACCAC+CGG | - | Chr7:79440040-79440059 | MsG0780040577.01.T01:CDS | 65.0% | |
| CCACCACCGGGCACCAATTG+GGG | - | Chr7:79440053-79440072 | MsG0780040577.01.T01:CDS | 65.0% | |
| CCCACCAGTTCCCGTGTGTG+TGG | + | Chr7:79440090-79440109 | None:intergenic | 65.0% | |
| CTGGTGGGGCTGCTCAAACG+AGG | - | Chr7:79440102-79440121 | MsG0780040577.01.T01:CDS | 65.0% | |
| GCCACCACCGGGCACCAATT+GGG | - | Chr7:79440052-79440071 | MsG0780040577.01.T01:CDS | 65.0% | |
| GGTGTCCACCCTGCCCCAAT+TGG | + | Chr7:79440069-79440088 | None:intergenic | 65.0% | |
| ! | ACCCTGCCCCAATTGGTGCC+CGG | + | Chr7:79440062-79440081 | None:intergenic | 65.0% |
| CACCGGGCACCAATTGGGGC+AGG | - | Chr7:79440057-79440076 | MsG0780040577.01.T01:CDS | 70.0% | |
| GGGCACCAATTGGGGCAGGG+TGG | - | Chr7:79440061-79440080 | MsG0780040577.01.T01:CDS | 70.0% | |
| ! | CCCCAATTGGTGCCCGGTGG+TGG | + | Chr7:79440056-79440075 | None:intergenic | 70.0% |
| ! | CTGCCCCAATTGGTGCCCGG+TGG | + | Chr7:79440059-79440078 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 79439944 | 79440162 | 79439944 | ID=MsG0780040577.01;Name=MsG0780040577.01 |
| Chr7 | mRNA | 79439944 | 79440162 | 79439944 | ID=MsG0780040577.01.T01;Parent=MsG0780040577.01;Name=MsG0780040577.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|72 |
| Chr7 | exon | 79439944 | 79440162 | 79439944 | ID=MsG0780040577.01.T01:exon:16288;Parent=MsG0780040577.01.T01 |
| Chr7 | CDS | 79439944 | 79440162 | 79439944 | ID=MsG0780040577.01.T01:cds;Parent=MsG0780040577.01.T01 |
| Gene Sequence |
| Protein sequence |
