AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780037662.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780037662.01.T01 MTR_7g076620 93.082 159 11 0 1 159 1 159 4.17e-107 302
MsG0780037662.01.T01 MTR_8g032260 49.296 142 65 3 1 135 1 142 1.25e-39 134
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780037662.01.T01 AT4G23890 49.593 123 55 3 16 134 17 136 2.82e-31 113

Find 28 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGTTTCCTGGTGGTGAAAA+AGG 0.216558 7:-30531276 MsG0780037662.01.T01:CDS
TTTATTTCTTCAATAGTAAA+AGG 0.269787 7:+30531207 None:intergenic
GCTCCTCTTCAAGGCCTTCA+TGG 0.281591 7:-30531618 MsG0780037662.01.T01:CDS
GGACTATGCAATGGAGAAAA+AGG 0.281632 7:-30531456 MsG0780037662.01.T01:CDS
GGGCTAACTGGAGGGTTTCC+TGG 0.314218 7:-30531288 MsG0780037662.01.T01:CDS
TCCTTCTCAAAACCATCTTT+AGG 0.343967 7:+30531318 None:intergenic
AAGGAAATGATGGGGCTAAC+TGG 0.367060 7:-30531300 MsG0780037662.01.T01:CDS
AGAGTTGAACTATGTTGCTT+TGG 0.385186 7:+30531164 None:intergenic
GGTGATCTTGGCCTATGAAC+TGG 0.404590 7:+30531580 None:intergenic
CAAGTTGATAAAGATGAAAC+AGG 0.427205 7:-30531411 MsG0780037662.01.T01:CDS
ATTGCTGCAATTCCTAAAGA+TGG 0.439610 7:-30531330 MsG0780037662.01.T01:CDS
CGTGGTTGTAGTTGAGAAGT+TGG 0.442826 7:+30531516 None:intergenic
GACTTGAACAAGAGCTGAAA+AGG 0.446004 7:-30531434 MsG0780037662.01.T01:CDS
CTCCTACGATCCCAGTTCAT+AGG 0.487970 7:-30531591 MsG0780037662.01.T01:CDS
TGAGAGTGAAGTGGTGATCT+TGG 0.497067 7:+30531568 None:intergenic
AAAGGTTGAGAAGAATTCCT+TGG 0.524418 7:+30531225 None:intergenic
GGTTGAGAAGAATTCCTTGG+TGG 0.533813 7:+30531228 None:intergenic
GAGCCATGAAGGCCTTGAAG+AGG 0.569908 7:+30531615 None:intergenic
GTGATCTTGGCCTATGAACT+GGG 0.572739 7:+30531581 None:intergenic
ATCGTAGGAGAGAGCCATGA+AGG 0.575050 7:+30531604 None:intergenic
AAATGATGGGGCTAACTGGA+GGG 0.586145 7:-30531296 MsG0780037662.01.T01:CDS
GGCCTATGAACTGGGATCGT+AGG 0.587300 7:+30531589 None:intergenic
GAAATGATGGGGCTAACTGG+AGG 0.588055 7:-30531297 MsG0780037662.01.T01:CDS
AAAGTGCAAGAAAACACAGA+TGG 0.600904 7:-30531375 MsG0780037662.01.T01:CDS
GGAGGAAGAGGACTATGCAA+TGG 0.605614 7:-30531465 MsG0780037662.01.T01:CDS
AAGATTTCATGAGAGTGAAG+TGG 0.619848 7:+30531559 None:intergenic
CTAACTGGAGGGTTTCCTGG+TGG 0.624925 7:-30531285 MsG0780037662.01.T01:CDS
AGTATGTTGAATTCAGCACG+TGG 0.711591 7:+30531498 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTTCTTCAATAGTAAA+AGG + Chr7:30531599-30531618 None:intergenic 15.0%
!! ATTCAACATACTAAAAATGA+TGG - Chr7:30531316-30531335 MsG0780037662.01.T01:CDS 20.0%
!! TTCAACATACTAAAAATGAT+GGG - Chr7:30531317-30531336 MsG0780037662.01.T01:CDS 20.0%
! AACATACTAAAAATGATGGG+AGG - Chr7:30531320-30531339 MsG0780037662.01.T01:CDS 30.0%
!! CAAGTTGATAAAGATGAAAC+AGG - Chr7:30531392-30531411 MsG0780037662.01.T01:CDS 30.0%
!!! GTTTTCTTGCACTTTTTCAT+TGG + Chr7:30531423-30531442 None:intergenic 30.0%
AAAGGTTGAGAAGAATTCCT+TGG + Chr7:30531581-30531600 None:intergenic 35.0%
AAAGTGCAAGAAAACACAGA+TGG - Chr7:30531428-30531447 MsG0780037662.01.T01:CDS 35.0%
AAGATTTCATGAGAGTGAAG+TGG + Chr7:30531247-30531266 None:intergenic 35.0%
ATTGCTGCAATTCCTAAAGA+TGG - Chr7:30531473-30531492 MsG0780037662.01.T01:CDS 35.0%
TCATTGAGAAAAATCCACCA+AGG - Chr7:30531561-30531580 MsG0780037662.01.T01:CDS 35.0%
TCCTTCTCAAAACCATCTTT+AGG + Chr7:30531488-30531507 None:intergenic 35.0%
!!! GGTTTTGAGAAGGAAATGAT+GGG - Chr7:30531494-30531513 MsG0780037662.01.T01:CDS 35.0%
!!! GTTTTGAGAAGGAAATGATG+GGG - Chr7:30531495-30531514 MsG0780037662.01.T01:CDS 35.0%
!!! TCCTAAAGATGGTTTTGAGA+AGG - Chr7:30531484-30531503 MsG0780037662.01.T01:CDS 35.0%
!!! TGGTTTTGAGAAGGAAATGA+TGG - Chr7:30531493-30531512 MsG0780037662.01.T01:CDS 35.0%
AGTATGTTGAATTCAGCACG+TGG + Chr7:30531308-30531327 None:intergenic 40.0%
GACTTGAACAAGAGCTGAAA+AGG - Chr7:30531369-30531388 MsG0780037662.01.T01:CDS 40.0%
GGACTATGCAATGGAGAAAA+AGG - Chr7:30531347-30531366 MsG0780037662.01.T01:CDS 40.0%
! CTAAAAATGATGGGAGGAAG+AGG - Chr7:30531326-30531345 MsG0780037662.01.T01:CDS 40.0%
CGTGGTTGTAGTTGAGAAGT+TGG + Chr7:30531290-30531309 None:intergenic 45.0%
GGTTGAGAAGAATTCCTTGG+TGG + Chr7:30531578-30531597 None:intergenic 45.0%
GTGATCTTGGCCTATGAACT+GGG + Chr7:30531225-30531244 None:intergenic 45.0%
TGAGAGTGAAGTGGTGATCT+TGG + Chr7:30531238-30531257 None:intergenic 45.0%
! AAATGATGGGGCTAACTGGA+GGG - Chr7:30531507-30531526 MsG0780037662.01.T01:CDS 45.0%
! AAGGAAATGATGGGGCTAAC+TGG - Chr7:30531503-30531522 MsG0780037662.01.T01:CDS 45.0%
! GGTTTCCTGGTGGTGAAAAA+GGG - Chr7:30531528-30531547 MsG0780037662.01.T01:CDS 45.0%
!! ATCAACCCTTTTTCACCACC+AGG + Chr7:30531536-30531555 None:intergenic 45.0%
ATCGTAGGAGAGAGCCATGA+AGG + Chr7:30531202-30531221 None:intergenic 50.0%
CTCCTACGATCCCAGTTCAT+AGG - Chr7:30531212-30531231 MsG0780037662.01.T01:CDS 50.0%
GGAGGAAGAGGACTATGCAA+TGG - Chr7:30531338-30531357 MsG0780037662.01.T01:CDS 50.0%
GGTGATCTTGGCCTATGAAC+TGG + Chr7:30531226-30531245 None:intergenic 50.0%
! GAAATGATGGGGCTAACTGG+AGG - Chr7:30531506-30531525 MsG0780037662.01.T01:CDS 50.0%
! GGGTTTCCTGGTGGTGAAAA+AGG - Chr7:30531527-30531546 MsG0780037662.01.T01:CDS 50.0%
!!! GCTGCTTTTGCTCCTCTTCA+AGG - Chr7:30531176-30531195 MsG0780037662.01.T01:CDS 50.0%
CTAACTGGAGGGTTTCCTGG+TGG - Chr7:30531518-30531537 MsG0780037662.01.T01:CDS 55.0%
GAGCCATGAAGGCCTTGAAG+AGG + Chr7:30531191-30531210 None:intergenic 55.0%
GCTCCTCTTCAAGGCCTTCA+TGG - Chr7:30531185-30531204 MsG0780037662.01.T01:CDS 55.0%
GGCCTATGAACTGGGATCGT+AGG + Chr7:30531217-30531236 None:intergenic 55.0%
GGGCTAACTGGAGGGTTTCC+TGG - Chr7:30531515-30531534 MsG0780037662.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 30531173 30531652 30531173 ID=MsG0780037662.01;Name=MsG0780037662.01
Chr7 mRNA 30531173 30531652 30531173 ID=MsG0780037662.01.T01;Parent=MsG0780037662.01;Name=MsG0780037662.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|159
Chr7 exon 30531173 30531652 30531173 ID=MsG0780037662.01.T01:exon:1661;Parent=MsG0780037662.01.T01
Chr7 CDS 30531173 30531652 30531173 ID=MsG0780037662.01.T01:cds;Parent=MsG0780037662.01.T01
Gene Sequence

>MsG0780037662.01.T01

ATGGCTGCTTTTGCTCCTCTTCAAGGCCTTCATGGCTCTCTCCTACGATCCCAGTTCATAGGCCAAGATCACCACTTCACTCTCATGAAATCTTCAACAACACATCACAGAAAACCAACTTCTCAACTACAACCACGTGCTGAATTCAACATACTAAAAATGATGGGAGGAAGAGGACTATGCAATGGAGAAAAAGGACTTGAACAAGAGCTGAAAAGGCAAGTTGATAAAGATGAAACAGGATCATCCAATGAAAAAGTGCAAGAAAACACAGATGGATCAGAAAAGTTGACTAGTGATATTGCTGCAATTCCTAAAGATGGTTTTGAGAAGGAAATGATGGGGCTAACTGGAGGGTTTCCTGGTGGTGAAAAAGGGTTGATTAAGTTCATTGAGAAAAATCCACCAAGGAATTCTTCTCAACCTTTTACTATTGAAGAAATAAATCAAAGTCTTGTTGAAGAATCCAAAGCAACATAG

Protein sequence

>MsG0780037662.01.T01

MAAFAPLQGLHGSLLRSQFIGQDHHFTLMKSSTTHHRKPTSQLQPRAEFNILKMMGGRGLCNGEKGLEQELKRQVDKDETGSSNEKVQENTDGSEKLTSDIAAIPKDGFEKEMMGLTGGFPGGEKGLIKFIEKNPPRNSSQPFTIEEINQSLVEESKAT*