Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043214.01.T01 | KEH18908.1 | 78.771 | 179 | 8 | 1 | 1 | 179 | 1 | 149 | 1.90E-86 | 277 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043214.01.T01 | A0A072TQ15 | 78.771 | 179 | 8 | 1 | 1 | 179 | 1 | 149 | 9.08e-87 | 277 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048515.01 | MsG0880043214.01 | 0.804581 | 2.017232e-49 | 1.043920e-46 |
| MsG0180004465.01 | MsG0880043214.01 | 0.800016 | 1.756324e-48 | 8.090712e-46 |
| MsG0180006054.01 | MsG0880043214.01 | 0.817837 | 2.680077e-52 | 1.973315e-49 |
| MsG0780040576.01 | MsG0880043214.01 | -0.827235 | 1.749890e-54 | 1.679413e-51 |
| MsG0780041229.01 | MsG0880043214.01 | 0.803930 | 2.755001e-49 | 1.402196e-46 |
| MsG0880042161.01 | MsG0880043214.01 | 0.830255 | 3.255244e-55 | 3.411718e-52 |
| MsG0880043214.01 | MsG0880043941.01 | 0.810502 | 1.116752e-50 | 6.742923e-48 |
| MsG0880043214.01 | MsG0880044572.01 | 0.825478 | 4.587086e-54 | 4.185628e-51 |
| MsG0880043214.01 | MsG0880044573.01 | 0.851233 | 1.013813e-60 | 2.038498e-57 |
| MsG0880043214.01 | MsG0880045992.01 | 0.804624 | 1.975829e-49 | 1.023599e-46 |
| MsG0280010916.01 | MsG0880043214.01 | 0.815432 | 9.272169e-52 | 6.391778e-49 |
| MsG0280011415.01 | MsG0880043214.01 | 0.811652 | 6.288532e-51 | 3.914441e-48 |
| MsG0380012085.01 | MsG0880043214.01 | 0.813142 | 2.973356e-51 | 1.926004e-48 |
| MsG0380013474.01 | MsG0880043214.01 | 0.831347 | 1.757231e-55 | 1.901777e-52 |
| MsG0380014727.01 | MsG0880043214.01 | 0.815946 | 7.122633e-52 | 4.979373e-49 |
| MsG0680033465.01 | MsG0880043214.01 | 0.806645 | 7.439687e-50 | 4.059172e-47 |
| MsG0680033639.01 | MsG0880043214.01 | 0.836292 | 1.019129e-56 | 1.278912e-53 |
| MsG0480018653.01 | MsG0880043214.01 | 0.814285 | 1.665482e-51 | 1.112897e-48 |
| MsG0480021869.01 | MsG0880043214.01 | 0.806244 | 9.039377e-50 | 4.882310e-47 |
| MsG0480023251.01 | MsG0880043214.01 | 0.818937 | 1.510014e-52 | 1.146152e-49 |
| MsG0480023488.01 | MsG0880043214.01 | 0.810333 | 1.214202e-50 | 7.298589e-48 |
| MsG0580024363.01 | MsG0880043214.01 | -0.808728 | 2.685226e-50 | 1.546947e-47 |
| MsG0580025723.01 | MsG0880043214.01 | 0.800689 | 1.281125e-48 | 6.003263e-46 |
| MsG0380015589.01 | MsG0880043214.01 | 0.823496 | 1.342805e-53 | 1.158131e-50 |
| MsG0380016391.01 | MsG0880043214.01 | 0.810225 | 1.281333e-50 | 7.680046e-48 |
| MsG0380017048.01 | MsG0880043214.01 | 0.803392 | 3.562514e-49 | 1.788140e-46 |
| MsG0380017220.01 | MsG0880043214.01 | 0.820856 | 5.498152e-53 | 4.401460e-50 |
| MsG0380017452.01 | MsG0880043214.01 | 0.810978 | 8.807223e-51 | 5.386642e-48 |
| MsG0480018236.01 | MsG0880043214.01 | 0.816853 | 4.462935e-52 | 3.198543e-49 |
| MsG0480018318.01 | MsG0880043214.01 | 0.846442 | 2.166075e-59 | 3.729144e-56 |
| MsG0480018402.01 | MsG0880043214.01 | 0.806349 | 8.586960e-50 | 4.650141e-47 |
| MsG0280007072.01 | MsG0880043214.01 | 0.802063 | 6.700374e-49 | 3.250974e-46 |
| MsG0280007496.01 | MsG0880043214.01 | 0.820786 | 5.707458e-53 | 4.559664e-50 |
| MsG0780038439.01 | MsG0880043214.01 | 0.826677 | 2.379431e-54 | 2.246714e-51 |
| MsG0780039178.01 | MsG0880043214.01 | 0.816626 | 5.020072e-52 | 3.575374e-49 |
| MsG0780040074.01 | MsG0880043214.01 | 0.815456 | 9.159152e-52 | 6.318181e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043214.01.T01 | MTR_8g031480 | 78.771 | 179 | 8 | 1 | 1 | 179 | 1 | 149 | 2.30e-90 | 277 |
| MsG0880043214.01.T01 | MTR_8g031480 | 78.771 | 179 | 8 | 1 | 1 | 179 | 1 | 149 | 2.81e-90 | 276 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880043214.01.T01 | AT1G01770 | 78.512 | 121 | 26 | 0 | 52 | 172 | 18 | 138 | 6.46e-62 | 202 |
Find 41 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACCTTATCCCTTCGCCTTT+TGG | 0.197722 | 8:-20729689 | None:intergenic |
| AACTTGCATAATTACCAATA+TGG | 0.275625 | 8:+20730353 | MsG0880043214.01.T01:CDS |
| GTTTCTAAAAGTGGATCCTC+TGG | 0.340116 | 8:+20730540 | MsG0880043214.01.T01:intron |
| ATTGGCTGTGGAGCAGGATT+TGG | 0.343927 | 8:+20729715 | MsG0880043214.01.T01:CDS |
| ATTCTTACTAGCACCCATAT+TGG | 0.353195 | 8:-20730367 | None:intergenic |
| GAGGTGTCTGTTACAAATTT+AGG | 0.356765 | 8:+20730631 | MsG0880043214.01.T01:CDS |
| ATTTGGCGGTGACAGGCCTT+TGG | 0.404822 | 8:+20729732 | MsG0880043214.01.T01:CDS |
| GAGCAGGATTTGGCGGTGAC+AGG | 0.410148 | 8:+20729725 | MsG0880043214.01.T01:CDS |
| TCCCAAAAGGCGAAGGGATA+AGG | 0.411944 | 8:+20729687 | MsG0880043214.01.T01:CDS |
| GATGAATATGCTGCTACCTC+TGG | 0.417107 | 8:+20730317 | MsG0880043214.01.T01:CDS |
| GTTTACATTGGCTGTGGAGC+AGG | 0.418506 | 8:+20729709 | MsG0880043214.01.T01:CDS |
| TACGCTCCGCCACGCATTCA+AGG | 0.429568 | 8:-20729801 | None:intergenic |
| CAATGTAATATCAACATTGT+TGG | 0.435431 | 8:-20729653 | None:intergenic |
| CAACATGATTTGATAACGTT+CGG | 0.437142 | 8:-20729830 | None:intergenic |
| TATGCTGCTACCTCTGGCTT+TGG | 0.444217 | 8:+20730323 | MsG0880043214.01.T01:CDS |
| TCATAATTGTTTGATAAAGC+TGG | 0.451330 | 8:+20729567 | MsG0880043214.01.T01:CDS |
| GGGTGCTCAACAAAAGGTCT+TGG | 0.456993 | 8:+20730561 | MsG0880043214.01.T01:CDS |
| TCCTCTGGGTGCTCAACAAA+AGG | 0.457650 | 8:+20730555 | MsG0880043214.01.T01:CDS |
| CGTTATCAAATCATGTTGTC+TGG | 0.471199 | 8:+20729835 | MsG0880043214.01.T01:CDS |
| ACTTGCATAATTACCAATAT+GGG | 0.471757 | 8:+20730354 | MsG0880043214.01.T01:CDS |
| TTGGAAATAGCTACCACACT+AGG | 0.472959 | 8:+20730580 | MsG0880043214.01.T01:CDS |
| TTCTGTTGCTGTTGCTCATG+AGG | 0.476035 | 8:+20730612 | MsG0880043214.01.T01:CDS |
| CTACCTCTGGCTTTGGAAAG+AGG | 0.498427 | 8:+20730330 | MsG0880043214.01.T01:CDS |
| TGTTGGTATTGTTGCTGCAA+TGG | 0.501537 | 8:-20729636 | None:intergenic |
| GGCTGTGGAGCAGGATTTGG+CGG | 0.503851 | 8:+20729718 | MsG0880043214.01.T01:CDS |
| TTTCTAAAAGTGGATCCTCT+GGG | 0.519682 | 8:+20730541 | MsG0880043214.01.T01:intron |
| GATAAGGTTTACATTGGCTG+TGG | 0.536128 | 8:+20729703 | MsG0880043214.01.T01:CDS |
| TGCAGAGAAGTAATCCCAAA+AGG | 0.539483 | 8:+20729674 | MsG0880043214.01.T01:CDS |
| CTCAATGGAAGTTGAAGCAA+TGG | 0.542464 | 8:+20729522 | None:intergenic |
| CGAAGGGATAAGGTTTACAT+TGG | 0.549338 | 8:+20729697 | MsG0880043214.01.T01:CDS |
| TATGAATCTCTTCTCTGTCG+TGG | 0.558550 | 8:-20729549 | None:intergenic |
| ATCATGTTGTCTGGTGGTGA+TGG | 0.558840 | 8:+20729844 | MsG0880043214.01.T01:CDS |
| GAAGTAATCCCAAAAGGCGA+AGG | 0.572871 | 8:+20729680 | MsG0880043214.01.T01:CDS |
| TGGAAATAGCTACCACACTA+GGG | 0.605065 | 8:+20730581 | MsG0880043214.01.T01:CDS |
| TTATCTAGTCCTTGAATGCG+TGG | 0.617578 | 8:+20729792 | MsG0880043214.01.T01:CDS |
| AAGTAATCCCAAAAGGCGAA+GGG | 0.634753 | 8:+20729681 | MsG0880043214.01.T01:CDS |
| AGAAACATCAAGCCCTAGTG+TGG | 0.638755 | 8:-20730593 | None:intergenic |
| GTTCCTCTTTCCAAAGCCAG+AGG | 0.656126 | 8:-20730333 | None:intergenic |
| AGCAACTTAAGAGCAGCCAA+AGG | 0.665595 | 8:-20729748 | None:intergenic |
| TATCAAATCATGTTGTCTGG+TGG | 0.722384 | 8:+20729838 | MsG0880043214.01.T01:CDS |
| TCTAGTCCTTGAATGCGTGG+CGG | 0.733181 | 8:+20729795 | MsG0880043214.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGAATTTGATTTAAATTTGA+TGG | + | Chr8:20730471-20730490 | MsG0880043214.01.T01:intron | 15.0% |
| !!! | TTTTTCCTTTTAAATTCAAT+AGG | + | Chr8:20729909-20729928 | MsG0880043214.01.T01:intron | 15.0% |
| !! | GAAAACCTATTGAATTTAAA+AGG | - | Chr8:20729917-20729936 | None:intergenic | 20.0% |
| !!! | TTTTTTAATTCTGCATTTGA+TGG | + | Chr8:20730389-20730408 | MsG0880043214.01.T01:intron | 20.0% |
| ! | AACTTGCATAATTACCAATA+TGG | + | Chr8:20730353-20730372 | MsG0880043214.01.T01:CDS | 25.0% |
| ! | ACAGTTAAAAAGACAACAAA+AGG | - | Chr8:20729611-20729630 | None:intergenic | 25.0% |
| ! | ACTTGCATAATTACCAATAT+GGG | + | Chr8:20730354-20730373 | MsG0880043214.01.T01:CDS | 25.0% |
| ! | CAATGTAATATCAACATTGT+TGG | - | Chr8:20729656-20729675 | None:intergenic | 25.0% |
| ! | TAAAAAGACAACAAAAGGTA+GGG | - | Chr8:20729606-20729625 | None:intergenic | 25.0% |
| ! | TCATAATTGTTTGATAAAGC+TGG | + | Chr8:20729567-20729586 | MsG0880043214.01.T01:CDS | 25.0% |
| ! | TTAAAAAGACAACAAAAGGT+AGG | - | Chr8:20729607-20729626 | None:intergenic | 25.0% |
| !! | TATTCAGTGCTATTAATTAG+CGG | + | Chr8:20730002-20730021 | MsG0880043214.01.T01:intron | 25.0% |
| !! | TTTTCTTTCTATCGTATACT+CGG | + | Chr8:20730191-20730210 | MsG0880043214.01.T01:intron | 25.0% |
| !!! | TCATTCTTTGTTTCTAAAAG+TGG | + | Chr8:20730531-20730550 | MsG0880043214.01.T01:intron | 25.0% |
| ATTGTGTTTAAGCATTTGCA+AGG | - | Chr8:20729963-20729982 | None:intergenic | 30.0% | |
| ! | AGCACTGAATAAAAATCATC+TGG | - | Chr8:20729993-20730012 | None:intergenic | 30.0% |
| ! | TATTTTCAACTAATCGCGAT+TGG | + | Chr8:20730154-20730173 | MsG0880043214.01.T01:intron | 30.0% |
| ! | TTTAAATTTGATGGACCTCA+TGG | + | Chr8:20730480-20730499 | MsG0880043214.01.T01:intron | 30.0% |
| !! | CAACATGATTTGATAACGTT+CGG | - | Chr8:20729833-20729852 | None:intergenic | 30.0% |
| !!! | TTTTATATGCAGTTTCTAGC+TGG | + | Chr8:20730295-20730314 | MsG0880043214.01.T01:intron | 30.0% |
| AACAAGTACAAGAAACCATG+AGG | - | Chr8:20730498-20730517 | None:intergenic | 35.0% | |
| ATGGTTATGATTCTCAGAGT+AGG | + | Chr8:20729863-20729882 | MsG0880043214.01.T01:intron | 35.0% | |
| ATTCTTACTAGCACCCATAT+TGG | - | Chr8:20730370-20730389 | None:intergenic | 35.0% | |
| CGTTATCAAATCATGTTGTC+TGG | + | Chr8:20729835-20729854 | MsG0880043214.01.T01:CDS | 35.0% | |
| GTAATAACACTGTAGTGTAG+CGG | + | Chr8:20730115-20730134 | MsG0880043214.01.T01:intron | 35.0% | |
| TATCAAATCATGTTGTCTGG+TGG | + | Chr8:20729838-20729857 | MsG0880043214.01.T01:CDS | 35.0% | |
| ! | GAGGTGTCTGTTACAAATTT+AGG | + | Chr8:20730631-20730650 | MsG0880043214.01.T01:CDS | 35.0% |
| ! | TCGCGATTAGTTGAAAATAG+TGG | - | Chr8:20730153-20730172 | None:intergenic | 35.0% |
| ! | TTTCTAAAAGTGGATCCTCT+GGG | + | Chr8:20730541-20730560 | MsG0880043214.01.T01:intron | 35.0% |
| AAGTAATCCCAAAAGGCGAA+GGG | + | Chr8:20729681-20729700 | MsG0880043214.01.T01:CDS | 40.0% | |
| CGAAGGGATAAGGTTTACAT+TGG | + | Chr8:20729697-20729716 | MsG0880043214.01.T01:CDS | 40.0% | |
| TATGAATCTCTTCTCTGTCG+TGG | - | Chr8:20729552-20729571 | None:intergenic | 40.0% | |
| TGCAGAGAAGTAATCCCAAA+AGG | + | Chr8:20729674-20729693 | MsG0880043214.01.T01:CDS | 40.0% | |
| TGGAAATAGCTACCACACTA+GGG | + | Chr8:20730581-20730600 | MsG0880043214.01.T01:CDS | 40.0% | |
| TGTTGGTATTGTTGCTGCAA+TGG | - | Chr8:20729639-20729658 | None:intergenic | 40.0% | |
| TTATCTAGTCCTTGAATGCG+TGG | + | Chr8:20729792-20729811 | MsG0880043214.01.T01:CDS | 40.0% | |
| TTGGAAATAGCTACCACACT+AGG | + | Chr8:20730580-20730599 | MsG0880043214.01.T01:CDS | 40.0% | |
| ! | GATAAGGTTTACATTGGCTG+TGG | + | Chr8:20729703-20729722 | MsG0880043214.01.T01:CDS | 40.0% |
| ! | GTTTCTAAAAGTGGATCCTC+TGG | + | Chr8:20730540-20730559 | MsG0880043214.01.T01:intron | 40.0% |
| AACTGAAAGTCTTGCCTGCA+AGG | - | Chr8:20730223-20730242 | None:intergenic | 45.0% | |
| AGAAACATCAAGCCCTAGTG+TGG | - | Chr8:20730596-20730615 | None:intergenic | 45.0% | |
| AGCAACTTAAGAGCAGCCAA+AGG | - | Chr8:20729751-20729770 | None:intergenic | 45.0% | |
| GAAGTAATCCCAAAAGGCGA+AGG | + | Chr8:20729680-20729699 | MsG0880043214.01.T01:CDS | 45.0% | |
| GATGAATATGCTGCTACCTC+TGG | + | Chr8:20730317-20730336 | MsG0880043214.01.T01:CDS | 45.0% | |
| ! | AACCTTATCCCTTCGCCTTT+TGG | - | Chr8:20729692-20729711 | None:intergenic | 45.0% |
| ! | ACCTTATCCCTTCGCCTTTT+GGG | - | Chr8:20729691-20729710 | None:intergenic | 45.0% |
| ! | ATCATGTTGTCTGGTGGTGA+TGG | + | Chr8:20729844-20729863 | MsG0880043214.01.T01:CDS | 45.0% |
| ! | TTCTGTTGCTGTTGCTCATG+AGG | + | Chr8:20730612-20730631 | MsG0880043214.01.T01:CDS | 45.0% |
| ATTGGCTGTGGAGCAGGATT+TGG | + | Chr8:20729715-20729734 | MsG0880043214.01.T01:CDS | 50.0% | |
| GGGTGCTCAACAAAAGGTCT+TGG | + | Chr8:20730561-20730580 | MsG0880043214.01.T01:CDS | 50.0% | |
| GTTCCTCTTTCCAAAGCCAG+AGG | - | Chr8:20730336-20730355 | None:intergenic | 50.0% | |
| TCCCAAAAGGCGAAGGGATA+AGG | + | Chr8:20729687-20729706 | MsG0880043214.01.T01:CDS | 50.0% | |
| TCCTCTGGGTGCTCAACAAA+AGG | + | Chr8:20730555-20730574 | MsG0880043214.01.T01:CDS | 50.0% | |
| TCTAGTCCTTGAATGCGTGG+CGG | + | Chr8:20729795-20729814 | MsG0880043214.01.T01:CDS | 50.0% | |
| ! | ACCTTTTGTTGAGCACCCAG+AGG | - | Chr8:20730559-20730578 | None:intergenic | 50.0% |
| ! | GTTTACATTGGCTGTGGAGC+AGG | + | Chr8:20729709-20729728 | MsG0880043214.01.T01:CDS | 50.0% |
| ! | TATGCTGCTACCTCTGGCTT+TGG | + | Chr8:20730323-20730342 | MsG0880043214.01.T01:CDS | 50.0% |
| !! | ATACTCGGTGTTAGCCTTGC+AGG | + | Chr8:20730206-20730225 | MsG0880043214.01.T01:intron | 50.0% |
| !! | CTACCTCTGGCTTTGGAAAG+AGG | + | Chr8:20730330-20730349 | MsG0880043214.01.T01:CDS | 50.0% |
| ATTTGGCGGTGACAGGCCTT+TGG | + | Chr8:20729732-20729751 | MsG0880043214.01.T01:CDS | 55.0% | |
| GAGCAGGATTTGGCGGTGAC+AGG | + | Chr8:20729725-20729744 | MsG0880043214.01.T01:CDS | 60.0% | |
| GGCTGTGGAGCAGGATTTGG+CGG | + | Chr8:20729718-20729737 | MsG0880043214.01.T01:CDS | 60.0% | |
| TACGCTCCGCCACGCATTCA+AGG | - | Chr8:20729804-20729823 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 20729526 | 20730660 | 20729526 | ID=MsG0880043214.01;Name=MsG0880043214.01 |
| Chr8 | mRNA | 20729526 | 20730660 | 20729526 | ID=MsG0880043214.01.T01;Parent=MsG0880043214.01;Name=MsG0880043214.01.T01;_AED=0.50;_eAED=0.58;_QI=0|1|0.33|1|1|1|3|0|179 |
| Chr8 | exon | 20729526 | 20729880 | 20729526 | ID=MsG0880043214.01.T01:exon:5553;Parent=MsG0880043214.01.T01 |
| Chr8 | exon | 20730307 | 20730381 | 20730307 | ID=MsG0880043214.01.T01:exon:5554;Parent=MsG0880043214.01.T01 |
| Chr8 | exon | 20730551 | 20730660 | 20730551 | ID=MsG0880043214.01.T01:exon:5555;Parent=MsG0880043214.01.T01 |
| Chr8 | CDS | 20729526 | 20729880 | 20729526 | ID=MsG0880043214.01.T01:cds;Parent=MsG0880043214.01.T01 |
| Chr8 | CDS | 20730307 | 20730381 | 20730307 | ID=MsG0880043214.01.T01:cds;Parent=MsG0880043214.01.T01 |
| Chr8 | CDS | 20730551 | 20730660 | 20730551 | ID=MsG0880043214.01.T01:cds;Parent=MsG0880043214.01.T01 |
| Gene Sequence |
| Protein sequence |
