AlfalfaGEDB | MsG0380017452.01

M. sativa cultivar ZhongmuNo.1 / MsG0380017452.01

KEGG: K01662
GO: GO:0008661 GO:0016114
PFAM: PF13292

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017452.01.T01	XP_003603440.1	98.507	201	3	0	1	201	508	708	8.23E-137	409
MsG0380017452.01.T02	MCI08956.1	94.048	84	2	1	132	212	8	91	1.87E-46	160
MsG0380017452.01.T03	PNY08129.1	72.68	194	12	1	9	161	127	320	8.97E-86	272

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017452.01.T01	O78328	68.657	201	62	1	1	200	519	719	5.34E-92	286
MsG0380017452.01.T02	O78328	58.14	86	33	1	132	214	154	239	1.19E-25	107
MsG0380017452.01.T03	Q38854	48.98	196	57	1	9	161	288	483	4.75E-53	182

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017452.01.T01	G7JBM5	98.507	201	3	0	1	201	508	708	3.93e-137	409
MsG0380017452.01.T02	A0A392PCD0	94.048	84	2	1	132	212	8	91	8.92e-47	160
MsG0380017452.01.T03	A0A072V1P9	76.804	194	4	1	9	161	278	471	1.33e-93	292

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048515.01	MsG0380017452.01	0.808101	3.654240e-50	2.070953e-47
MsG0080048769.01	MsG0380017452.01	0.859884	3.036229e-63	8.143450e-60
MsG0180000042.01	MsG0380017452.01	0.809530	1.807622e-50	1.063936e-47
MsG0180000091.01	MsG0380017452.01	0.850345	1.802885e-60	3.520600e-57
MsG0180000470.01	MsG0380017452.01	0.819311	1.241154e-52	9.516634e-50
MsG0180000555.01	MsG0380017452.01	0.818454	1.943517e-52	1.455574e-49
MsG0180000604.01	MsG0380017452.01	0.822721	2.035764e-53	1.717764e-50
MsG0180000649.01	MsG0380017452.01	0.810561	1.084229e-50	6.556971e-48
MsG0180000702.01	MsG0380017452.01	0.817204	3.722149e-52	2.693325e-49
MsG0180000741.01	MsG0380017452.01	0.813440	2.556437e-51	1.669627e-48

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017452.01.T01	MTR_3g107740	98.507	201	3	0	1	201	508	708	9.97e-141	409
MsG0380017452.01.T01	MTR_4g118640	69.500	200	60	1	1	199	472	671	1.58e-92	284
MsG0380017452.01.T01	MTR_4g118640	69.500	200	60	1	1	199	517	716	4.06e-92	284
MsG0380017452.01.T01	MTR_2g020590	66.337	202	67	1	1	201	515	716	4.21e-90	279
MsG0380017452.01.T01	MTR_8g068300	63.317	199	72	1	1	198	512	710	4.43e-84	263
MsG0380017452.01.T01	MTR_8g068265	62.814	199	73	1	1	198	512	710	6.52e-83	260
MsG0380017452.01.T01	MTR_4g118640	68.519	162	50	1	1	161	517	678	2.35e-69	223
MsG0380017452.01.T01	MTR_3g107740	84.615	65	10	0	1	65	508	572	9.59e-31	118
MsG0380017452.01.T02	MTR_3g107740	95.349	86	1	1	132	214	146	231	3.19e-48	166
MsG0380017452.01.T02	MTR_3g107740	95.349	86	1	1	132	214	146	231	1.87e-47	166

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017452.01.T01	AT5G11380	75.377	199	49	0	1	199	501	699	5.13e-105	317
MsG0380017452.01.T01	AT3G21500	67.513	197	63	1	1	196	478	674	8.01e-89	275
MsG0380017452.01.T01	AT4G15560	67.513	197	63	1	1	196	520	716	6.13e-86	268
MsG0380017452.01.T01	AT3G21500	66.447	152	50	1	1	151	477	628	4.65e-64	209
MsG0380017452.01.T01	AT3G21500	66.447	152	50	1	1	151	478	629	5.19e-64	209
MsG0380017452.01.T01	AT5G11380	57.143	63	27	0	1	63	501	563	1.99e-18	83.2
MsG0380017452.01.T01	AT5G11380	61.818	55	21	0	1	55	501	555	5.28e-17	79.0
MsG0380017452.01.T02	AT5G11380	72.368	76	18	1	142	214	166	241	5.38e-29	113
MsG0380017452.01.T02	AT5G11380	72.368	76	18	1	142	214	166	241	6.13e-29	113
MsG0380017452.01.T02	AT5G11380	72.368	76	18	1	142	214	166	241	7.53e-29	113

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Find 217 sgRNAs with CRISPR-Local

Find 683 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAGTGTCAAATAGAAA+GGG	0.457000	3:-96398407	MsG0380017452.01.T01:three_prime_UTR
AAAGACGAAGCTATACATGA+TGG	0.597974	3:-96399402	MsG0380017452.01.T01:CDS
AAAGAGTGTCAAATAGAAAG+GGG	0.648956	3:-96398406	MsG0380017452.01.T01:three_prime_UTR
AAAGGTTCTTCAAGCAAAGA+GGG	0.533111	3:+96405345	None:intergenic
AAAGTTGATGAATATGCTCG+TGG	0.672546	3:-96402074	MsG0380017452.01.T01:five_prime_UTR
AAAGTTTAGAGAAGCTGCTA+AGG	0.557892	3:-96404422	MsG0380017452.01.T01:intron
AAATAGAAGACAACTCTAAA+CGG	0.479369	3:+96405288	None:intergenic
AAATATACTGAATCCTAGTA+TGG	0.374702	3:-96405253	MsG0380017452.01.T01:five_prime_UTR
AACAGTATATCTGCTGGACT+TGG	0.544844	3:-96404896	MsG0380017452.01.T01:intron
AACATATGGTGATTGCTTTG+TGG	0.497898	3:-96400784	MsG0380017452.01.T01:five_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAAAAATCTGGAGGAAGA+TGG	+	Chr3:96401906-96401925	None:intergenic	30.0%
AAAAAATCCTCCAAATTTGG+GGG	+	Chr3:96405956-96405975	None:intergenic	30.0%
AAACATTGCAACACATATAG+AGG	+	Chr3:96404350-96404369	None:intergenic	30.0%
AACTCTTGCACATTACATAA+TGG	-	Chr3:96406185-96406204	MsG0380017452.01.T02:intron	30.0%
AAGAAGAATACAAGGTGATT+TGG	+	Chr3:96400432-96400451	None:intergenic	30.0%
AAGAATGCAAAAGGCTTATT+AGG	-	Chr3:96407372-96407391	MsG0380017452.01.T02:intron	30.0%
AAGTAGCTTCTTTGATTCAA+TGG	-	Chr3:96403947-96403966	MsG0380017452.01.T01:intron	30.0%
AATAAAAAGAAGGGATGATG+TGG	+	Chr3:96401811-96401830	None:intergenic	30.0%
AATAGATGTGTTATGGTGTT+TGG	+	Chr3:96399291-96399310	None:intergenic	30.0%
ACAACCAATTAGAAAGTGAT+TGG	+	Chr3:96405030-96405049	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	96398268	96407648	96398268	ID=MsG0380017452.01;Name=MsG0380017452.01
Chr3	mRNA	96398268	96405665	96398268	ID=MsG0380017452.01.T01;Parent=MsG0380017452.01;Name=MsG0380017452.01.T01;_AED=0.27;_eAED=0.27;_QI=1592\|1\|1\|1\|0.28\|0.25\|8\|327\|201
Chr3	exon	96398268	96398747	96398268	ID=MsG0380017452.01.T01:exon:17562;Parent=MsG0380017452.01.T01
Chr3	exon	96398928	96399212	96398928	ID=MsG0380017452.01.T01:exon:17561;Parent=MsG0380017452.01.T01
Chr3	exon	96399389	96399666	96399389	ID=MsG0380017452.01.T01:exon:17560;Parent=MsG0380017452.01.T01
Chr3	exon	96400557	96400849	96400557	ID=MsG0380017452.01.T01:exon:17559;Parent=MsG0380017452.01.T01
Chr3	exon	96401862	96402141	96401862	ID=MsG0380017452.01.T01:exon:17558;Parent=MsG0380017452.01.T01
Chr3	exon	96404423	96404688	96404423	ID=MsG0380017452.01.T01:exon:17557;Parent=MsG0380017452.01.T01
Chr3	exon	96404897	96405047	96404897	ID=MsG0380017452.01.T01:exon:17556;Parent=MsG0380017452.01.T01
Chr3	exon	96405174	96405665	96405174	ID=MsG0380017452.01.T01:exon:17555;Parent=MsG0380017452.01.T01
Chr3	five_prime_UTR	96405174	96405665	96405174	ID=MsG0380017452.01.T01:five_prime_utr;Parent=MsG0380017452.01.T01
Chr3	five_prime_UTR	96404897	96405047	96404897	ID=MsG0380017452.01.T01:five_prime_utr;Parent=MsG0380017452.01.T01
Chr3	five_prime_UTR	96404423	96404688	96404423	ID=MsG0380017452.01.T01:five_prime_utr;Parent=MsG0380017452.01.T01
Chr3	five_prime_UTR	96401862	96402141	96401862	ID=MsG0380017452.01.T01:five_prime_utr;Parent=MsG0380017452.01.T01
Chr3	five_prime_UTR	96400557	96400849	96400557	ID=MsG0380017452.01.T01:five_prime_utr;Parent=MsG0380017452.01.T01
Chr3	five_prime_UTR	96399557	96399666	96399557	ID=MsG0380017452.01.T01:five_prime_utr;Parent=MsG0380017452.01.T01
Chr3	CDS	96399389	96399556	96399389	ID=MsG0380017452.01.T01:cds;Parent=MsG0380017452.01.T01
Chr3	CDS	96398928	96399212	96398928	ID=MsG0380017452.01.T01:cds;Parent=MsG0380017452.01.T01
Chr3	CDS	96398595	96398747	96398595	ID=MsG0380017452.01.T01:cds;Parent=MsG0380017452.01.T01
Chr3	three_prime_UTR	96398268	96398594	96398268	ID=MsG0380017452.01.T01:three_prime_utr;Parent=MsG0380017452.01.T01
Chr3	mRNA	96404555	96407648	96404555	ID=MsG0380017452.01.T02;Parent=MsG0380017452.01;Name=MsG0380017452.01.T02;_AED=0.59;_eAED=0.59;_QI=0\|0\|0\|0.75\|0.33\|0.5\|4\|0\|215
Chr3	exon	96407381	96407648	96407381	ID=MsG0380017452.01.T02:exon:17566;Parent=MsG0380017452.01.T02
Chr3	exon	96407176	96407304	96407176	ID=MsG0380017452.01.T02:exon:17565;Parent=MsG0380017452.01.T02
Chr3	exon	96404897	96405022	96404897	ID=MsG0380017452.01.T02:exon:17564;Parent=MsG0380017452.01.T02
Chr3	exon	96404555	96404679	96404555	ID=MsG0380017452.01.T02:exon:17563;Parent=MsG0380017452.01.T02
Chr3	CDS	96407381	96407648	96407381	ID=MsG0380017452.01.T02:cds;Parent=MsG0380017452.01.T02
Chr3	CDS	96407176	96407304	96407176	ID=MsG0380017452.01.T02:cds;Parent=MsG0380017452.01.T02
Chr3	CDS	96404897	96405022	96404897	ID=MsG0380017452.01.T02:cds;Parent=MsG0380017452.01.T02
Chr3	CDS	96404555	96404679	96404555	ID=MsG0380017452.01.T02:cds;Parent=MsG0380017452.01.T02
Chr3	mRNA	96400509	96402168	96400509	ID=MsG0380017452.01.T03;Parent=MsG0380017452.01;Name=MsG0380017452.01.T03;_AED=0.45;_eAED=0.50;_QI=0\|0\|0\|1\|1\|1\|2\|0\|175
Chr3	exon	96401982	96402168	96401982	ID=MsG0380017452.01.T03:exon:17568;Parent=MsG0380017452.01.T03
Chr3	exon	96400509	96400849	96400509	ID=MsG0380017452.01.T03:exon:17567;Parent=MsG0380017452.01.T03
Chr3	CDS	96401982	96402168	96401982	ID=MsG0380017452.01.T03:cds;Parent=MsG0380017452.01.T03
Chr3	CDS	96400509	96400849	96400509	ID=MsG0380017452.01.T03:cds;Parent=MsG0380017452.01.T03

Gene Sequence

>MsG0380017452.01.T01

ATGTCATGCTTACCAAATATGATTGTCATGGCACCATCTGATGAGGCTGAACTTGTGCACATGGTGGCGACTGCTGCTCGTATCAATGATCAACCAGTTTGCTTTCGGTATCCTAGGGGTGCCTTGGTTGGGAAAGACGAAGCTATACATGATGGAATTCCAATTGAGATTGGAAAGGGAAGAATTCTTGTTGAAGGTAAAGATGTTGCCTTATTGGGATATGGATCAATGGTTCAGAACTGTCTAAAGGCTCATTCACTTCTTGCAAATCTTGGCATTGAGGTGACTGTTGCTGATGCAAGGTTCTGCAAACCCCTTGACATCGAGCTTCTGAGGCAGCTTTGTAAACACCATTCTTTTCTAATTACCGTTGAGGAAGGGTCTATTGGAGGATTTGGTTCCCATGTTGCTCAATTTATTGCTCTTGATGGACTTCTCGACCGAAGAATTAAGTGGCGACCAATTGTTTTACCAGACAGTTATATTGAACATGCATCACCAAATCAACAGCTTAACCAAGCAGGGTTGACTGGACATCACATTGCTGCCACAGCATTGAGTTTGCTGGGCCGTACTCGTGAAGCTCTATCCTTTATGTGTTTGTGA

>MsG0380017452.01.T02

TTGCTCTTCCTATTTTGCATCGCAGTGCCTTTATCTTTCGTGTTTGAAGAAGGATGCAACAGTAGTGCGTCACGGAGGCTTACACCAATCTCACCTGGAAGATATGTTCTGAAGCATCGCAGAGATTCAATGAATGGTGACCATAGTTATAATAATGGTCGATGTTCAAACTATCAGCATGTCAGCTATATGGAGTGCAACGAGAATGTAGAAGACTCTTATGTCCCTGACAATAATCACGGTATGTGTTTGCGCATTGAATTTTTTAAGATCAACGACATTGTTCATGACGAAACTATGTCATCTGATATTATATTTAAGTTGGATGATAATCAAGACGTGGACGTAAATTGTGACGAAAATGGAAAAATAGATGACGTGCCTGCTAATGATTCTGGACGGAGATCGCTCATGAAGACAATAAGAAAAAAGAATGGTCTTTCTGGTTTTACTTCTCGATTCGAGAGTGAATACGATGCATTTGGTGCAGGTCATGGATGCAACAGTATATCTGCTGGACTTGTTGCAAGGGATATTAAAGGGAGACGAGAACGTGTAGTTGCAGTTATCAGCAATTGGACAACTATGTCTGGCCAGGTCTATGAGGCAATGAGCAATGCAGGTTACTTGGACTCAAATTTGTGGTAA

>MsG0380017452.01.T03

ATGATCCCCACTTCTGCTTTGCCACAGGGTGTTACAAAACGTATTGGCAGAGGCATGCATGAATTAGCAGCTAAAGTTGATGAATATGCTCGTGGTATGATGGGTCCACCAGGTTCTACTCTATTTGAAGAGCTTGGGTTGTATTACATAGGCCCAGTGGATGGACACAATATCGAAGATCTAATAAAATTGGTTAGTTCTGATTTGTTAGACAATGCTGGTCGGCTTCAAACATATGGTGATTGCTTTGTGGAGAGTTTGGTAGCCGAAGCTGAGAAAGACAAAGACATTGTGGTTGTTCATGCAGGAATCACGACAGAGCCGTCACTTAAACTGTTTATGGAAAAATTTCCAGATAGGATTTTTAATGTGGGAATTGCTGAGCAACACGCTGTCACATTTGCTTCTGGTTTATCATGTGGTGGATTGAAGCCATTTTGCATTATACCTTCTTCCTTTTTACAGAGAGCCTATGACCAGGTTCATACTTATTTCAGCTTAAGCTATCATGATAACCACTATATGTAG

Protein sequence

>MsG0380017452.01.T01

MSCLPNMIVMAPSDEAELVHMVATAARINDQPVCFRYPRGALVGKDEAIHDGIPIEIGKGRILVEGKDVALLGYGSMVQNCLKAHSLLANLGIEVTVADARFCKPLDIELLRQLCKHHSFLITVEEGSIGGFGSHVAQFIALDGLLDRRIKWRPIVLPDSYIEHASPNQQLNQAGLTGHHIAATALSLLGRTREALSFMCL*

>MsG0380017452.01.T02

LLFLFCIAVPLSFVFEEGCNSSASRRLTPISPGRYVLKHRRDSMNGDHSYNNGRCSNYQHVSYMECNENVEDSYVPDNNHGMCLRIEFFKINDIVHDETMSSDIIFKLDDNQDVDVNCDENGKIDDVPANDSGRRSLMKTIRKKNGLSGFTSRFESEYDAFGAGHGCNSISAGLVARDIKGRRERVVAVISNWTTMSGQVYEAMSNAGYLDSNLW*

>MsG0380017452.01.T03

MIPTSALPQGVTKRIGRGMHELAAKVDEYARGMMGPPGSTLFEELGLYYIGPVDGHNIEDLIKLVSSDLLDNAGRLQTYGDCFVESLVAEAEKDKDIVVVHAGITTEPSLKLFMEKFPDRIFNVGIAEQHAVTFASGLSCGGLKPFCIIPSSFLQRAYDQVHTYFSLSYHDNHYM*