Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046756.01.T01 | XP_013458085.3 | 96.296 | 108 | 4 | 0 | 8 | 115 | 75 | 182 | 1.96E-67 | 211 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046756.01.T01 | A0A072URX6 | 96.296 | 108 | 4 | 0 | 8 | 115 | 75 | 182 | 9.34e-68 | 211 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048964.01 | MsG0880046756.01 | 0.809428 | 1.901496e-50 | 1.116156e-47 |
| MsG0180000235.01 | MsG0880046756.01 | 0.807473 | 4.969559e-50 | 2.770546e-47 |
| MsG0180000503.01 | MsG0880046756.01 | 0.805526 | 1.279194e-49 | 6.782800e-47 |
| MsG0180003671.01 | MsG0880046756.01 | 0.803045 | 4.203917e-49 | 2.091304e-46 |
| MsG0180003864.01 | MsG0880046756.01 | 0.803721 | 3.044640e-49 | 1.541166e-46 |
| MsG0180004180.01 | MsG0880046756.01 | 0.803968 | 2.706380e-49 | 1.378694e-46 |
| MsG0180004372.01 | MsG0880046756.01 | 0.803208 | 3.890848e-49 | 1.943693e-46 |
| MsG0180005842.01 | MsG0880046756.01 | 0.826059 | 3.339808e-54 | 3.098461e-51 |
| MsG0180005878.01 | MsG0880046756.01 | 0.818665 | 1.741474e-52 | 1.311661e-49 |
| MsG0180005993.01 | MsG0880046756.01 | 0.819369 | 1.204267e-52 | 9.248690e-50 |
| MsG0180006016.01 | MsG0880046756.01 | 0.800769 | 1.233609e-48 | 5.792295e-46 |
| MsG0180006138.01 | MsG0880046756.01 | 0.805255 | 1.457976e-49 | 7.676949e-47 |
| MsG0780040442.01 | MsG0880046756.01 | 0.805292 | 1.432322e-49 | 7.549323e-47 |
| MsG0780041065.01 | MsG0880046756.01 | 0.835310 | 1.807718e-56 | 2.202372e-53 |
| MsG0680034037.01 | MsG0880046756.01 | 0.805106 | 1.566500e-49 | 8.217253e-47 |
| MsG0480019816.01 | MsG0880046756.01 | 0.812222 | 4.725485e-51 | 2.987285e-48 |
| MsG0480020065.01 | MsG0880046756.01 | 0.800124 | 1.669346e-48 | 7.711343e-46 |
| MsG0480020090.01 | MsG0880046756.01 | 0.805740 | 1.153797e-49 | 6.152078e-47 |
| MsG0480021953.01 | MsG0880046756.01 | 0.829139 | 6.083155e-55 | 6.171415e-52 |
| MsG0480022467.01 | MsG0880046756.01 | 0.803047 | 4.200137e-49 | 2.089520e-46 |
| MsG0480022551.01 | MsG0880046756.01 | 0.805303 | 1.424512e-49 | 7.510331e-47 |
| MsG0480023014.01 | MsG0880046756.01 | 0.839166 | 1.864465e-57 | 2.553193e-54 |
| MsG0480023025.01 | MsG0880046756.01 | 0.810391 | 1.179955e-50 | 7.103428e-48 |
| MsG0480023088.01 | MsG0880046756.01 | 0.803674 | 3.115080e-49 | 1.574847e-46 |
| MsG0480023224.01 | MsG0880046756.01 | 0.804318 | 2.288403e-49 | 1.176246e-46 |
| MsG0580024353.01 | MsG0880046756.01 | 0.808346 | 3.239951e-50 | 1.848143e-47 |
| MsG0580025989.01 | MsG0880046756.01 | 0.805846 | 1.095901e-49 | 5.859207e-47 |
| MsG0380016581.01 | MsG0880046756.01 | 0.808774 | 2.625574e-50 | 1.514389e-47 |
| MsG0380016624.01 | MsG0880046756.01 | 0.806374 | 8.485799e-50 | 4.598292e-47 |
| MsG0380017140.01 | MsG0880046756.01 | 0.816082 | 6.643231e-52 | 4.661850e-49 |
| MsG0580029762.01 | MsG0880046756.01 | 0.810824 | 9.514109e-51 | 5.794442e-48 |
| MsG0280007088.01 | MsG0880046756.01 | 0.855627 | 5.557528e-62 | 1.291071e-58 |
| MsG0280007636.01 | MsG0880046756.01 | 0.835079 | 2.067673e-56 | 2.501101e-53 |
| MsG0280007648.01 | MsG0880046756.01 | 0.810296 | 1.236653e-50 | 7.426261e-48 |
| MsG0280007815.01 | MsG0880046756.01 | 0.819291 | 1.254215e-52 | 9.611578e-50 |
| MsG0280008556.01 | MsG0880046756.01 | 0.811881 | 5.606746e-51 | 3.511470e-48 |
| MsG0780039144.01 | MsG0880046756.01 | 0.821230 | 4.508260e-53 | 3.646734e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046756.01.T01 | MTR_4g114105 | 96.296 | 108 | 4 | 0 | 8 | 115 | 75 | 182 | 2.37e-71 | 211 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046756.01.T01 | AT5G20120 | 62.963 | 108 | 34 | 2 | 8 | 115 | 87 | 188 | 1.84e-30 | 107 |
| MsG0880046756.01.T01 | AT5G20120 | 62.963 | 108 | 34 | 2 | 8 | 115 | 87 | 188 | 1.84e-30 | 107 |
Find 26 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTTGCTTTGTGGCCAATT+TGG | 0.157438 | 8:+77716436 | MsG0880046756.01.T01:CDS |
| TTTCTTGACGATTCCTCTTC+TGG | 0.249847 | 8:+77716461 | MsG0880046756.01.T01:CDS |
| CTAGGTTCACCTTTGCTTTG+TGG | 0.328652 | 8:+77716427 | MsG0880046756.01.T01:intron |
| TGTGCAGTTTACACTATTCA+TGG | 0.346494 | 8:+77716585 | MsG0880046756.01.T01:intron |
| CCTCTATATCTTGGGATGAT+AGG | 0.347964 | 8:+77716161 | MsG0880046756.01.T01:CDS |
| TGCAGACTGCCTCTATATCT+TGG | 0.356363 | 8:+77716152 | MsG0880046756.01.T01:CDS |
| TTGCCATTCGCCACTGGATT+CGG | 0.365198 | 8:+77716296 | MsG0880046756.01.T01:CDS |
| TTTCATTTATACAAGTATCT+TGG | 0.379944 | 8:+77716205 | MsG0880046756.01.T01:CDS |
| TATCTTGGGATGATAGGTAT+TGG | 0.427837 | 8:+77716167 | MsG0880046756.01.T01:CDS |
| GTTTGTTTCTTACAAGCAAA+AGG | 0.450655 | 8:-77716327 | None:intergenic |
| ATCGTCAAGAAACTCCAAAT+TGG | 0.465863 | 8:-77716450 | None:intergenic |
| TTTATACAAGTATCTTGGCT+TGG | 0.469511 | 8:+77716210 | MsG0880046756.01.T01:CDS |
| GCAGACTGCCTCTATATCTT+GGG | 0.469604 | 8:+77716153 | MsG0880046756.01.T01:CDS |
| AAAATGGGGATTAAAGAAAT+GGG | 0.471320 | 8:+77716253 | MsG0880046756.01.T01:CDS |
| CCTATCATCCCAAGATATAG+AGG | 0.476285 | 8:-77716161 | None:intergenic |
| ACAGTGTTGCCATTCGCCAC+TGG | 0.512752 | 8:+77716290 | MsG0880046756.01.T01:CDS |
| TCTTGGCTTGGAGCGTTCGA+AGG | 0.539897 | 8:+77716222 | MsG0880046756.01.T01:CDS |
| GGAATTCATCATTCTGAGGC+CGG | 0.559074 | 8:-77716651 | None:intergenic |
| TACACTATTCATGGCATGCA+TGG | 0.561683 | 8:+77716594 | MsG0880046756.01.T01:CDS |
| TTGTTGATACATACCAGAAG+AGG | 0.562376 | 8:-77716474 | None:intergenic |
| ATTCCGAATCCAGTGGCGAA+TGG | 0.603263 | 8:-77716299 | None:intergenic |
| CCAAATTGGCCACAAAGCAA+AGG | 0.609872 | 8:-77716436 | None:intergenic |
| GTACGGAATTCATCATTCTG+AGG | 0.654930 | 8:-77716655 | None:intergenic |
| TGTTTCTTACAAGCAAAAGG+AGG | 0.657267 | 8:-77716324 | None:intergenic |
| ACTATTCATGGCATGCATGG+TGG | 0.676070 | 8:+77716597 | MsG0880046756.01.T01:CDS |
| GGAGGTGATTCCGAATCCAG+TGG | 0.689356 | 8:-77716306 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTTAGAGGAAAAATAAC+AGG | - | Chr8:77715671-77715690 | None:intergenic | 20.0% |
| !! | AAGTAATAAAACATAGTAAG+CGG | - | Chr8:77716510-77716529 | None:intergenic | 20.0% |
| !! | AATTTAGAGGAAAAATAACA+GGG | - | Chr8:77715670-77715689 | None:intergenic | 20.0% |
| !! | TACTTATATAATAGGAAAGA+GGG | - | Chr8:77715966-77715985 | None:intergenic | 20.0% |
| !! | TTTCATTTATACAAGTATCT+TGG | + | Chr8:77716205-77716224 | MsG0880046756.01.T01:CDS | 20.0% |
| !!! | GTTATCACAATTTCAATTTT+TGG | - | Chr8:77716546-77716565 | None:intergenic | 20.0% |
| !!! | TATGAACTGATTTTATGTTT+AGG | - | Chr8:77715811-77715830 | None:intergenic | 20.0% |
| !!! | TGTTATTTTTCCTCTAAATT+TGG | + | Chr8:77715670-77715689 | MsG0880046756.01.T01:intron | 20.0% |
| !!! | TGTTTAAAATCCAAATTTAG+AGG | - | Chr8:77715683-77715702 | None:intergenic | 20.0% |
| ! | AAAAATGGGGATTAAAGAAA+TGG | + | Chr8:77716252-77716271 | MsG0880046756.01.T01:CDS | 25.0% |
| ! | AAAATGGGGATTAAAGAAAT+GGG | + | Chr8:77716253-77716272 | MsG0880046756.01.T01:CDS | 25.0% |
| ! | ATCTTAAATTTCATCAAACC+GGG | - | Chr8:77715989-77716008 | None:intergenic | 25.0% |
| ! | CATCATATTTGAAAAGCAAA+TGG | - | Chr8:77715622-77715641 | None:intergenic | 25.0% |
| ! | CATCTTAAATTTCATCAAAC+CGG | - | Chr8:77715990-77716009 | None:intergenic | 25.0% |
| ! | GATTATGAAAATTCCGATAT+TGG | - | Chr8:77716116-77716135 | None:intergenic | 25.0% |
| ! | GTACTTATATAATAGGAAAG+AGG | - | Chr8:77715967-77715986 | None:intergenic | 25.0% |
| ! | TTAAGATAAACAGTATCCAA+CGG | + | Chr8:77715905-77715924 | MsG0880046756.01.T01:intron | 25.0% |
| ! | TTTCCTATTATATAAGTACC+CGG | + | Chr8:77715968-77715987 | MsG0880046756.01.T01:intron | 25.0% |
| !!! | TAATCCCTTATCTCATTTTT+GGG | + | Chr8:77716620-77716639 | MsG0880046756.01.T01:CDS | 25.0% |
| AAACCGGGTACTTATATAAT+AGG | - | Chr8:77715974-77715993 | None:intergenic | 30.0% | |
| AAATTTCGATATCGTCAAAC+AGG | - | Chr8:77716025-77716044 | None:intergenic | 30.0% | |
| AACTCATATCTGATTAAACC+AGG | - | Chr8:77715855-77715874 | None:intergenic | 30.0% | |
| ACTTGACTTCATACCAATAT+CGG | + | Chr8:77716100-77716119 | MsG0880046756.01.T01:intron | 30.0% | |
| AGAATCCCAAAAATGAGATA+AGG | - | Chr8:77716628-77716647 | None:intergenic | 30.0% | |
| CAATTGGATGTGGAAATTAT+GGG | + | Chr8:77715725-77715744 | MsG0880046756.01.T01:intron | 30.0% | |
| CATATTTGAAAAGCAAATGG+TGG | - | Chr8:77715619-77715638 | None:intergenic | 30.0% | |
| GAATCCCAAAAATGAGATAA+GGG | - | Chr8:77716627-77716646 | None:intergenic | 30.0% | |
| TATATAATAGGAAAGAGGGA+AGG | - | Chr8:77715962-77715981 | None:intergenic | 30.0% | |
| TCAATTGGATGTGGAAATTA+TGG | + | Chr8:77715724-77715743 | MsG0880046756.01.T01:intron | 30.0% | |
| TCGAAGGTTTGATGAAAAAT+GGG | + | Chr8:77716238-77716257 | MsG0880046756.01.T01:CDS | 30.0% | |
| TTCGAAGGTTTGATGAAAAA+TGG | + | Chr8:77716237-77716256 | MsG0880046756.01.T01:CDS | 30.0% | |
| TTTATACAAGTATCTTGGCT+TGG | + | Chr8:77716210-77716229 | MsG0880046756.01.T01:CDS | 30.0% | |
| ! | GTAATCCCTTATCTCATTTT+TGG | + | Chr8:77716619-77716638 | MsG0880046756.01.T01:CDS | 30.0% |
| ! | GTTTGTTTCTTACAAGCAAA+AGG | - | Chr8:77716330-77716349 | None:intergenic | 30.0% |
| !!! | TATCTCATTTTTGGGATTCT+TGG | + | Chr8:77716628-77716647 | MsG0880046756.01.T01:CDS | 30.0% |
| ATCGTCAAGAAACTCCAAAT+TGG | - | Chr8:77716453-77716472 | None:intergenic | 35.0% | |
| ATTCACTTCAAAACATCCGT+TGG | - | Chr8:77715924-77715943 | None:intergenic | 35.0% | |
| ATTTCCACATCCAATTGACA+AGG | - | Chr8:77715722-77715741 | None:intergenic | 35.0% | |
| CGAAGGTTTGATGAAAAATG+GGG | + | Chr8:77716239-77716258 | MsG0880046756.01.T01:CDS | 35.0% | |
| TATCTTGGGATGATAGGTAT+TGG | + | Chr8:77716167-77716186 | MsG0880046756.01.T01:CDS | 35.0% | |
| TGTAAACTGCACAACATAAG+AGG | - | Chr8:77716578-77716597 | None:intergenic | 35.0% | |
| TGTGCAGTTTACACTATTCA+TGG | + | Chr8:77716585-77716604 | MsG0880046756.01.T01:intron | 35.0% | |
| TGTTTCTTACAAGCAAAAGG+AGG | - | Chr8:77716327-77716346 | None:intergenic | 35.0% | |
| TTGTTGATACATACCAGAAG+AGG | - | Chr8:77716477-77716496 | None:intergenic | 35.0% | |
| TTTCCACATCCAATTGACAA+GGG | - | Chr8:77715721-77715740 | None:intergenic | 35.0% | |
| ! | TTTTGCTTACTCCCTATCTT+TGG | + | Chr8:77716399-77716418 | MsG0880046756.01.T01:intron | 35.0% |
| !!! | ACGGATGTTTTGAAGTGAAT+CGG | + | Chr8:77715924-77715943 | MsG0880046756.01.T01:intron | 35.0% |
| AACCTAGAGATGCCAAAGAT+AGG | - | Chr8:77716414-77716433 | None:intergenic | 40.0% | |
| ACCTAGAGATGCCAAAGATA+GGG | - | Chr8:77716413-77716432 | None:intergenic | 40.0% | |
| AGATAGGGAGTAAGCAAAAC+AGG | - | Chr8:77716398-77716417 | None:intergenic | 40.0% | |
| CAAAACATGATGTCAGTTCC+TGG | + | Chr8:77715834-77715853 | MsG0880046756.01.T01:intron | 40.0% | |
| CCTATCATCCCAAGATATAG+AGG | - | Chr8:77716164-77716183 | None:intergenic | 40.0% | |
| CCTCTATATCTTGGGATGAT+AGG | + | Chr8:77716161-77716180 | MsG0880046756.01.T01:CDS | 40.0% | |
| GATAGGGAGTAAGCAAAACA+GGG | - | Chr8:77716397-77716416 | None:intergenic | 40.0% | |
| GTACGGAATTCATCATTCTG+AGG | - | Chr8:77716658-77716677 | None:intergenic | 40.0% | |
| TACACTATTCATGGCATGCA+TGG | + | Chr8:77716594-77716613 | MsG0880046756.01.T01:CDS | 40.0% | |
| TGATTGATGCCCTTGTCAAT+TGG | + | Chr8:77715709-77715728 | MsG0880046756.01.T01:intron | 40.0% | |
| TTTCTTGACGATTCCTCTTC+TGG | + | Chr8:77716461-77716480 | MsG0880046756.01.T01:CDS | 40.0% | |
| !!! | TCATTTTTGGGATTCTTGGC+CGG | + | Chr8:77716632-77716651 | MsG0880046756.01.T01:CDS | 40.0% |
| ACTATTCATGGCATGCATGG+TGG | + | Chr8:77716597-77716616 | MsG0880046756.01.T01:CDS | 45.0% | |
| ATGCCCTTGTCAATTGGATG+TGG | + | Chr8:77715715-77715734 | MsG0880046756.01.T01:intron | 45.0% | |
| CCAAATTGGCCACAAAGCAA+AGG | - | Chr8:77716439-77716458 | None:intergenic | 45.0% | |
| CCTTTGCTTTGTGGCCAATT+TGG | + | Chr8:77716436-77716455 | MsG0880046756.01.T01:CDS | 45.0% | |
| CTAGGTTCACCTTTGCTTTG+TGG | + | Chr8:77716427-77716446 | MsG0880046756.01.T01:intron | 45.0% | |
| GCAGACTGCCTCTATATCTT+GGG | + | Chr8:77716153-77716172 | MsG0880046756.01.T01:CDS | 45.0% | |
| GGCAGTCTGCAATAGAACTA+AGG | - | Chr8:77716143-77716162 | None:intergenic | 45.0% | |
| TCCCTATCTTTGGCATCTCT+AGG | + | Chr8:77716409-77716428 | MsG0880046756.01.T01:intron | 45.0% | |
| TCGATATCGTCAAACAGGTG+CGG | - | Chr8:77716020-77716039 | None:intergenic | 45.0% | |
| TGCAGACTGCCTCTATATCT+TGG | + | Chr8:77716152-77716171 | MsG0880046756.01.T01:CDS | 45.0% | |
| !! | GGAATTCATCATTCTGAGGC+CGG | - | Chr8:77716654-77716673 | None:intergenic | 45.0% |
| ATAAGACCAGACCATGAGGC+AGG | + | Chr8:77715750-77715769 | MsG0880046756.01.T01:intron | 50.0% | |
| ATTCCGAATCCAGTGGCGAA+TGG | - | Chr8:77716302-77716321 | None:intergenic | 50.0% | |
| GGTCATAAGACCAGACCATG+AGG | + | Chr8:77715746-77715765 | MsG0880046756.01.T01:intron | 50.0% | |
| TAAGACCAGACCATGAGGCA+GGG | + | Chr8:77715751-77715770 | MsG0880046756.01.T01:intron | 50.0% | |
| TTGCCATTCGCCACTGGATT+CGG | + | Chr8:77716296-77716315 | MsG0880046756.01.T01:CDS | 50.0% | |
| ACAGTGTTGCCATTCGCCAC+TGG | + | Chr8:77716290-77716309 | MsG0880046756.01.T01:CDS | 55.0% | |
| GGAGGTGATTCCGAATCCAG+TGG | - | Chr8:77716309-77716328 | None:intergenic | 55.0% | |
| !! | TCTTGGCTTGGAGCGTTCGA+AGG | + | Chr8:77716222-77716241 | MsG0880046756.01.T01:CDS | 55.0% |
| ACGTGCCCTGCCTCATGGTC+TGG | - | Chr8:77715759-77715778 | None:intergenic | 65.0% | |
| TGCACACGTGCCCTGCCTCA+TGG | - | Chr8:77715764-77715783 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 77715591 | 77716684 | 77715591 | ID=MsG0880046756.01;Name=MsG0880046756.01 |
| Chr8 | mRNA | 77715591 | 77716684 | 77715591 | ID=MsG0880046756.01.T01;Parent=MsG0880046756.01;Name=MsG0880046756.01.T01;_AED=0.50;_eAED=0.53;_QI=0|0|0|1|0.66|0.5|4|0|115 |
| Chr8 | exon | 77715591 | 77715602 | 77715591 | ID=MsG0880046756.01.T01:exon:33236;Parent=MsG0880046756.01.T01 |
| Chr8 | exon | 77716146 | 77716336 | 77716146 | ID=MsG0880046756.01.T01:exon:33237;Parent=MsG0880046756.01.T01 |
| Chr8 | exon | 77716431 | 77716482 | 77716431 | ID=MsG0880046756.01.T01:exon:33238;Parent=MsG0880046756.01.T01 |
| Chr8 | exon | 77716592 | 77716684 | 77716592 | ID=MsG0880046756.01.T01:exon:33239;Parent=MsG0880046756.01.T01 |
| Chr8 | CDS | 77715591 | 77715602 | 77715591 | ID=MsG0880046756.01.T01:cds;Parent=MsG0880046756.01.T01 |
| Chr8 | CDS | 77716146 | 77716336 | 77716146 | ID=MsG0880046756.01.T01:cds;Parent=MsG0880046756.01.T01 |
| Chr8 | CDS | 77716431 | 77716482 | 77716431 | ID=MsG0880046756.01.T01:cds;Parent=MsG0880046756.01.T01 |
| Chr8 | CDS | 77716592 | 77716684 | 77716592 | ID=MsG0880046756.01.T01:cds;Parent=MsG0880046756.01.T01 |
| Gene Sequence |
| Protein sequence |
