Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047324.01.T01 | MCI28067.1 | 87.755 | 49 | 6 | 0 | 19 | 67 | 37 | 85 | 3.04E-22 | 91.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047324.01.T01 | O65282 | 70 | 50 | 15 | 0 | 18 | 67 | 204 | 253 | 3.36E-17 | 75.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047324.01.T01 | A0A2K3NWG2 | 87.755 | 49 | 6 | 0 | 19 | 67 | 149 | 197 | 2.82e-22 | 94.0 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000355.01 | MsG0880047324.01 | 0.802452 | 5.573850e-49 | 2.731303e-46 |
| MsG0180001270.01 | MsG0880047324.01 | 0.810484 | 1.126473e-50 | 6.798488e-48 |
| MsG0880045101.01 | MsG0880047324.01 | 0.816813 | 4.556255e-52 | 3.261803e-49 |
| MsG0880046267.01 | MsG0880047324.01 | 0.811262 | 7.646374e-51 | 4.711110e-48 |
| MsG0880046765.01 | MsG0880047324.01 | 0.815384 | 9.504966e-52 | 6.543438e-49 |
| MsG0880047324.01 | MsG0880047325.01 | 0.880611 | 4.759591e-70 | 2.701573e-66 |
| MsG0880047324.01 | MsG0880047587.01 | 0.858412 | 8.387275e-63 | 2.138739e-59 |
| MsG0780041291.01 | MsG0880047324.01 | 0.808511 | 2.988081e-50 | 1.711745e-47 |
| MsG0480018644.01 | MsG0880047324.01 | 0.813887 | 2.038140e-51 | 1.347339e-48 |
| MsG0480020758.01 | MsG0880047324.01 | 0.823963 | 1.043512e-53 | 9.119655e-51 |
| MsG0480022587.01 | MsG0880047324.01 | 0.847170 | 1.369882e-59 | 2.414141e-56 |
| MsG0480022647.01 | MsG0880047324.01 | 0.804285 | 2.324358e-49 | 1.193749e-46 |
| MsG0480023009.01 | MsG0880047324.01 | 0.802215 | 6.236122e-49 | 3.037372e-46 |
| MsG0480023314.01 | MsG0880047324.01 | 0.832269 | 1.040736e-55 | 1.157279e-52 |
| MsG0480023909.01 | MsG0880047324.01 | 0.810597 | 1.065240e-50 | 6.448346e-48 |
| MsG0380015352.01 | MsG0880047324.01 | 0.835206 | 1.919990e-56 | 2.331708e-53 |
| MsG0380015902.01 | MsG0880047324.01 | 0.818553 | 1.846368e-52 | 1.386460e-49 |
| MsG0380017703.01 | MsG0880047324.01 | 0.827140 | 1.844465e-54 | 1.765176e-51 |
| MsG0380017785.01 | MsG0880047324.01 | 0.850843 | 1.306807e-60 | 2.594112e-57 |
| MsG0380017968.01 | MsG0880047324.01 | 0.845683 | 3.486319e-59 | 5.856238e-56 |
| MsG0380018048.01 | MsG0880047324.01 | 0.801840 | 7.447391e-49 | 3.593039e-46 |
| MsG0280008388.01 | MsG0880047324.01 | 0.814455 | 1.527581e-51 | 1.025465e-48 |
| MsG0280008394.01 | MsG0880047324.01 | 0.811074 | 8.395403e-51 | 5.147236e-48 |
| MsG0280008451.01 | MsG0880047324.01 | 0.817224 | 3.684674e-52 | 2.667631e-49 |
| MsG0280009384.01 | MsG0880047324.01 | 0.824199 | 9.184317e-54 | 8.079921e-51 |
| MsG0280009981.01 | MsG0880047324.01 | -0.827604 | 1.427127e-54 | 1.384286e-51 |
| MsG0780038348.01 | MsG0880047324.01 | 0.807386 | 5.184064e-50 | 2.883567e-47 |
| MsG0780038351.01 | MsG0880047324.01 | 0.801064 | 1.074023e-48 | 5.081210e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047324.01.T01 | MTR_4g124910 | 89.796 | 49 | 5 | 0 | 19 | 67 | 205 | 253 | 9.37e-25 | 92.4 |
| MsG0880047324.01.T01 | MTR_2g043230 | 75.510 | 49 | 12 | 0 | 19 | 67 | 206 | 254 | 1.14e-20 | 81.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047324.01.T01 | AT5G20720 | 70.000 | 50 | 15 | 0 | 18 | 67 | 204 | 253 | 3.42e-18 | 75.1 |
| MsG0880047324.01.T01 | AT5G20720 | 70.000 | 50 | 15 | 0 | 18 | 67 | 204 | 253 | 3.42e-18 | 75.1 |
| MsG0880047324.01.T01 | AT5G20720 | 70.000 | 50 | 15 | 0 | 18 | 67 | 204 | 253 | 3.42e-18 | 75.1 |
| MsG0880047324.01.T01 | AT5G20720 | 70.000 | 50 | 15 | 0 | 18 | 67 | 204 | 253 | 3.42e-18 | 75.1 |
Find 24 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGCTATCCTCTCTTAATTT+TGG | 0.185301 | 8:-84706698 | MsG0880047324.01.T01:exon |
| CCCTCAACACCACAAAATTT+AGG | 0.252909 | 8:+84706657 | None:intergenic |
| GAAGTCATTCCCTGCATATT+TGG | 0.259970 | 8:+84706770 | None:intergenic |
| AACTGTATTCCCGGGTGAAA+TGG | 0.321202 | 8:+84706800 | None:intergenic |
| TTCCCGGGTGAAATGGATAA+TGG | 0.363835 | 8:+84706807 | None:intergenic |
| GGAGTACAAAACTGTATTCC+CGG | 0.420138 | 8:+84706791 | None:intergenic |
| CAGGTGATAGCTGTTGGTCC+AGG | 0.453335 | 8:-84706857 | MsG0880047324.01.T01:CDS |
| GCCTTCGTCATCCACAGCCC+TGG | 0.454299 | 8:+84706839 | None:intergenic |
| ATAATTTACTAACATCCATC+TGG | 0.463942 | 8:+84706447 | None:intergenic |
| TAGCTGTTGGTCCAGGGCTG+TGG | 0.468827 | 8:-84706850 | MsG0880047324.01.T01:CDS |
| AATGACTTCAAAGGCAAAGA+TGG | 0.508584 | 8:-84706756 | MsG0880047324.01.T01:CDS |
| TATGCAGGGAATGACTTCAA+AGG | 0.569312 | 8:-84706765 | MsG0880047324.01.T01:CDS |
| GAGTACAAAACTGTATTCCC+GGG | 0.572521 | 8:+84706792 | None:intergenic |
| AGGTGATAGCTGTTGGTCCA+GGG | 0.579593 | 8:-84706856 | MsG0880047324.01.T01:CDS |
| AAACCATTATCCATTTCACC+CGG | 0.592297 | 8:-84706810 | MsG0880047324.01.T01:CDS |
| TGTTATATCGTATGAAGGGA+AGG | 0.592402 | 8:-84707179 | None:intergenic |
| GCAATGTTATATCGTATGAA+GGG | 0.615801 | 8:-84707183 | None:intergenic |
| AAAATAGCCAAAATTAAGAG+AGG | 0.622578 | 8:+84706691 | None:intergenic |
| AACCATTATCCATTTCACCC+GGG | 0.641591 | 8:-84706809 | MsG0880047324.01.T01:CDS |
| GATGCAACAGAGATTGTGCA+AGG | 0.653011 | 8:+84706569 | None:intergenic |
| TCCAGGGCTGTGGATGACGA+AGG | 0.668091 | 8:-84706840 | MsG0880047324.01.T01:CDS |
| ATGCATTGAAAACCATGAGA+AGG | 0.671326 | 8:+84706515 | None:intergenic |
| ATGTAACAGGTGATAGCTGT+TGG | 0.691284 | 8:-84706863 | MsG0880047324.01.T01:intron |
| AGAGGATAGCCATGACATCG+AGG | 0.712192 | 8:+84706709 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GTTTTCAAAAATTAAAACAA+TGG | - | Chr8:84706478-84706497 | MsG0880047324.01.T01:three_prime_UTR | 15.0% |
| !! | TCAAAAATTAAAACAATGGA+TGG | - | Chr8:84706482-84706501 | MsG0880047324.01.T01:three_prime_UTR | 20.0% |
| ! | AAAATAGCCAAAATTAAGAG+AGG | + | Chr8:84706915-84706934 | None:intergenic | 25.0% |
| ! | ATAATTTACTAACATCCATC+TGG | + | Chr8:84707159-84707178 | None:intergenic | 25.0% |
| !! | GTGTTTTGTATTATTGTCAA+CGG | - | Chr8:84707058-84707077 | MsG0880047324.01.T01:intron | 25.0% |
| !! | TTTATTTATTGCTTTAGCTC+AGG | - | Chr8:84706625-84706644 | MsG0880047324.01.T01:three_prime_UTR | 25.0% |
| !!! | GTTTTTTACATATATCCAGA+TGG | - | Chr8:84707141-84707160 | MsG0880047324.01.T01:intron | 25.0% |
| !!! | TCAATTTTTATTTTGCAACG+AGG | - | Chr8:84706975-84706994 | MsG0880047324.01.T01:intron | 25.0% |
| AACTAGCATTATCCTTAAGA+CGG | - | Chr8:84706561-84706580 | MsG0880047324.01.T01:three_prime_UTR | 30.0% | |
| AATTAAAACAATGGATGGTC+TGG | - | Chr8:84706487-84706506 | MsG0880047324.01.T01:three_prime_UTR | 30.0% | |
| AGAATTGCAAGAATGTGATT+TGG | - | Chr8:84706452-84706471 | MsG0880047324.01.T01:three_prime_UTR | 30.0% | |
| ATGTAAAAAACTGTTCTCGT+TGG | + | Chr8:84707132-84707151 | None:intergenic | 30.0% | |
| GAATGGTTATTTACATACCT+AGG | + | Chr8:84706708-84706727 | None:intergenic | 30.0% | |
| GAATTGCAAGAATGTGATTT+GGG | - | Chr8:84706453-84706472 | MsG0880047324.01.T01:three_prime_UTR | 30.0% | |
| ! | AATAACCATTCTACTCCTTT+TGG | - | Chr8:84706717-84706736 | MsG0880047324.01.T01:CDS | 30.0% |
| ! | GTTGTAGAACCTAAATTTTG+TGG | - | Chr8:84706937-84706956 | MsG0880047324.01.T01:intron | 30.0% |
| ! | TTTTGTACTCCAAATATGCA+GGG | - | Chr8:84706824-84706843 | MsG0880047324.01.T01:CDS | 30.0% |
| !!! | TTGTTTTGATCGAGTAGTTT+TGG | - | Chr8:84706681-84706700 | MsG0880047324.01.T01:three_prime_UTR | 30.0% |
| AAACCATTATCCATTTCACC+CGG | - | Chr8:84706793-84706812 | MsG0880047324.01.T01:CDS | 35.0% | |
| AATGACTTCAAAGGCAAAGA+TGG | - | Chr8:84706847-84706866 | MsG0880047324.01.T01:CDS | 35.0% | |
| ATGCATTGAAAACCATGAGA+AGG | + | Chr8:84707091-84707110 | None:intergenic | 35.0% | |
| CATTCTATTCTACCGTCTTA+AGG | + | Chr8:84706576-84706595 | None:intergenic | 35.0% | |
| TACATCCAAAAGGAGTAGAA+TGG | + | Chr8:84706725-84706744 | None:intergenic | 35.0% | |
| TATCACCTGTTACATCCAAA+AGG | + | Chr8:84706735-84706754 | None:intergenic | 35.0% | |
| ! | GTTTTGTACTCCAAATATGC+AGG | - | Chr8:84706823-84706842 | MsG0880047324.01.T01:CDS | 35.0% |
| ! | TGGCTATCCTCTCTTAATTT+TGG | - | Chr8:84706905-84706924 | MsG0880047324.01.T01:intron | 35.0% |
| !! | ACCTAAATTTTGTGGTGTTG+AGG | - | Chr8:84706945-84706964 | MsG0880047324.01.T01:intron | 35.0% |
| !! | CCTAAATTTTGTGGTGTTGA+GGG | - | Chr8:84706946-84706965 | MsG0880047324.01.T01:intron | 35.0% |
| AACCATTATCCATTTCACCC+GGG | - | Chr8:84706794-84706813 | MsG0880047324.01.T01:CDS | 40.0% | |
| AGGATAATGCTAGTTGCTAC+AGG | + | Chr8:84706556-84706575 | None:intergenic | 40.0% | |
| ATGTAACAGGTGATAGCTGT+TGG | - | Chr8:84706740-84706759 | MsG0880047324.01.T01:CDS | 40.0% | |
| CCCTCAACACCACAAAATTT+AGG | + | Chr8:84706949-84706968 | None:intergenic | 40.0% | |
| GAAGTCATTCCCTGCATATT+TGG | + | Chr8:84706836-84706855 | None:intergenic | 40.0% | |
| GAGTACAAAACTGTATTCCC+GGG | + | Chr8:84706814-84706833 | None:intergenic | 40.0% | |
| GGAGTACAAAACTGTATTCC+CGG | + | Chr8:84706815-84706834 | None:intergenic | 40.0% | |
| TATGCAGGGAATGACTTCAA+AGG | - | Chr8:84706838-84706857 | MsG0880047324.01.T01:CDS | 40.0% | |
| ! | CTACTCCTTTTGGATGTAAC+AGG | - | Chr8:84706727-84706746 | MsG0880047324.01.T01:CDS | 40.0% |
| ! | TATTGCTTTAGCTCAGGTCA+TGG | - | Chr8:84706631-84706650 | MsG0880047324.01.T01:three_prime_UTR | 40.0% |
| AACTGTATTCCCGGGTGAAA+TGG | + | Chr8:84706806-84706825 | None:intergenic | 45.0% | |
| GATGCAACAGAGATTGTGCA+AGG | + | Chr8:84707037-84707056 | None:intergenic | 45.0% | |
| TTCCCGGGTGAAATGGATAA+TGG | + | Chr8:84706799-84706818 | None:intergenic | 45.0% | |
| ! | AACGGCTTTTCACCTTCTCA+TGG | - | Chr8:84707076-84707095 | MsG0880047324.01.T01:intron | 45.0% |
| ! | CTTTAGCTCAGGTCATGGTT+TGG | - | Chr8:84706636-84706655 | MsG0880047324.01.T01:three_prime_UTR | 45.0% |
| !! | TTTTGAGAGCCTCGATGTCA+TGG | - | Chr8:84706885-84706904 | MsG0880047324.01.T01:intron | 45.0% |
| !!! | GATCGAGTAGTTTTGGTCCT+AGG | - | Chr8:84706688-84706707 | MsG0880047324.01.T01:exon | 45.0% |
| AGAGGATAGCCATGACATCG+AGG | + | Chr8:84706897-84706916 | None:intergenic | 50.0% | |
| AGGTGATAGCTGTTGGTCCA+GGG | - | Chr8:84706747-84706766 | MsG0880047324.01.T01:CDS | 50.0% | |
| CAGGTGATAGCTGTTGGTCC+AGG | - | Chr8:84706746-84706765 | MsG0880047324.01.T01:CDS | 55.0% | |
| TAGCTGTTGGTCCAGGGCTG+TGG | - | Chr8:84706753-84706772 | MsG0880047324.01.T01:CDS | 60.0% | |
| TCCAGGGCTGTGGATGACGA+AGG | - | Chr8:84706763-84706782 | MsG0880047324.01.T01:CDS | 60.0% | |
| GCCTTCGTCATCCACAGCCC+TGG | + | Chr8:84706767-84706786 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 84706435 | 84707190 | 84706435 | ID=MsG0880047324.01;Name=MsG0880047324.01 |
| Chr8 | mRNA | 84706435 | 84707190 | 84706435 | ID=MsG0880047324.01.T01;Parent=MsG0880047324.01;Name=MsG0880047324.01.T01;_AED=0.50;_eAED=0.53;_QI=0|0|0.5|1|0|0|2|269|67 |
| Chr8 | exon | 84706435 | 84706876 | 84706435 | ID=MsG0880047324.01.T01:exon:1591;Parent=MsG0880047324.01.T01 |
| Chr8 | exon | 84707160 | 84707190 | 84707160 | ID=MsG0880047324.01.T01:exon:1590;Parent=MsG0880047324.01.T01 |
| Chr8 | CDS | 84707160 | 84707190 | 84707160 | ID=MsG0880047324.01.T01:cds;Parent=MsG0880047324.01.T01 |
| Chr8 | CDS | 84706704 | 84706876 | 84706704 | ID=MsG0880047324.01.T01:cds;Parent=MsG0880047324.01.T01 |
| Chr8 | three_prime_UTR | 84706435 | 84706703 | 84706435 | ID=MsG0880047324.01.T01:three_prime_utr;Parent=MsG0880047324.01.T01 |
| Gene Sequence |
| Protein sequence |
