AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280009384.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009384.01.T02 MTR_2g061490 92.771 83 6 0 8 90 41 123 5.70e-49 151
MsG0280009384.01.T02 MTR_2g061510 92.771 83 6 0 8 90 41 123 5.70e-49 151
MsG0280009384.01.T01 MTR_2g061490 87.903 124 14 1 1 124 1 123 1.14e-71 210
MsG0280009384.01.T01 MTR_2g061510 87.903 124 14 1 1 124 1 123 1.14e-71 210
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 41 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTCCTCTCCTTACAATTT+TGG 0.174141 2:-51164778 MsG0280009384.01.T01:CDS
AGATCACAATACCTCAAATT+AGG 0.206664 2:-51165793 MsG0280009384.01.T01:CDS
TTCCTTGTTTGTTGGGTCAT+TGG 0.234460 2:-51164738 MsG0280009384.01.T01:CDS
TGATATTAAATGGCGTCTAT+TGG 0.294643 2:-51165844 MsG0280009384.01.T01:exon
ACAAACAAACATGTTTATTT+CGG 0.303916 2:+51164669 None:intergenic
TCCTTGTTTGTTGGGTCATT+GGG 0.315553 2:-51164737 MsG0280009384.01.T01:CDS
TGGGTCATTGGGTCCGTTGT+AGG 0.319973 2:-51164726 MsG0280009384.01.T01:CDS
CTTTACATCTGATCCTTCAT+TGG 0.334627 2:+51164824 None:intergenic
AGACAGACAGTGATATTAAA+TGG 0.354093 2:-51165854 MsG0280009384.01.T01:exon
GGAAACTGGCAATGCTTCTC+AGG 0.356908 2:-51164802 MsG0280009384.01.T01:CDS
TCAACTTCTCTCCCACCTTC+AGG 0.366725 2:-51165732 MsG0280009384.01.T01:intron
GGATCAGATGTAAAGGAAAC+TGG 0.407614 2:-51164816 MsG0280009384.01.T01:CDS
GATCACAATACCTCAAATTA+GGG 0.410926 2:-51165792 MsG0280009384.01.T01:CDS
ATTTCTAGAGCCTATGCATC+TGG 0.433233 2:+51164914 None:intergenic
GTGGCAATGGGAAGGAATTG+AGG 0.459199 2:+51165757 None:intergenic
GGGTCATTGGGTCCGTTGTA+GGG 0.469514 2:-51164725 MsG0280009384.01.T01:CDS
GAGAAGTTGAGTGTGGCAAT+GGG 0.472153 2:+51165745 None:intergenic
AGAGTCCAGCCAAAATTGTA+AGG 0.489275 2:+51164769 None:intergenic
AGAGAAGTTGAGTGTGGCAA+TGG 0.517362 2:+51165744 None:intergenic
CAATGAAGGATCAGATGTAA+AGG 0.522407 2:-51164823 MsG0280009384.01.T01:CDS
TTGAAGGTAACCCTAATTTG+AGG 0.528710 2:+51165782 None:intergenic
TGGATTCTCCTCACTGTCAC+CGG 0.535382 2:-51165824 MsG0280009384.01.T01:CDS
AAACATGTTTATTTCGGAGA+GGG 0.543120 2:+51164675 None:intergenic
GGTGGAAAATCATCCAATGA+AGG 0.556794 2:-51164837 MsG0280009384.01.T01:CDS
AGTTGAGTGTGGCAATGGGA+AGG 0.564752 2:+51165749 None:intergenic
AATGTATTATCCAGATGCAT+AGG 0.565409 2:-51164924 MsG0280009384.01.T01:intron
GCCAAAATTGTAAGGAGAGG+AGG 0.568215 2:+51164777 None:intergenic
AAAGATAAGGACTAATACAA+TGG 0.575529 2:+51164618 None:intergenic
ACCCAATGACCCAACAAACA+AGG 0.579860 2:+51164736 None:intergenic
ACAAACCCCAACTCCAGTGG+TGG 0.594457 2:-51164855 MsG0280009384.01.T01:CDS
GAAACTGGCAATGCTTCTCA+GGG 0.603720 2:-51164801 MsG0280009384.01.T01:CDS
TCATTGGGTCCGTTGTAGGG+TGG 0.604108 2:-51164722 MsG0280009384.01.T01:CDS
CCAGCCAAAATTGTAAGGAG+AGG 0.629021 2:+51164774 None:intergenic
GGTGGGAGAGAAGTTGAGTG+TGG 0.637036 2:+51165738 None:intergenic
CAAACATGTTTATTTCGGAG+AGG 0.642713 2:+51164674 None:intergenic
TATTGTGATCTGACTGTCGC+CGG 0.646250 2:+51165805 None:intergenic
GCATGAACCAGACCTGAAGG+TGG 0.646924 2:+51165720 None:intergenic
CATGAACCAGACCTGAAGGT+GGG 0.649807 2:+51165721 None:intergenic
GCTACAAACCCCAACTCCAG+TGG 0.660305 2:-51164858 MsG0280009384.01.T01:CDS
ACTTATCAACCACCCTACAA+CGG 0.666808 2:+51164713 None:intergenic
GACTGTCGCCGGTGACAGTG+AGG 0.666827 2:+51165816 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAAACAAACATGTTTATTT+CGG + Chr2:51165799-51165818 None:intergenic 20.0%
!! TATTACTACTTAAAAAACCT+TGG + Chr2:51164856-51164875 None:intergenic 20.0%
!!! ATCTCTCATTTCAAAATTTT+AGG - Chr2:51164794-51164813 MsG0280009384.01.T01:CDS 20.0%
!!! TAACATGTTTGTTTTGATTA+AGG + Chr2:51165395-51165414 None:intergenic 20.0%
!!! TGAAATTTTGTTTATGTCAT+TGG + Chr2:51165250-51165269 None:intergenic 20.0%
!!! TGAATTGATTTTGAAATGTT+TGG - Chr2:51164980-51164999 MsG0280009384.01.T01:intron 20.0%
! AAAGATAAGGACTAATACAA+TGG + Chr2:51165850-51165869 None:intergenic 25.0%
! TAGCAAAAAAAAGTAAAGCA+AGG + Chr2:51164775-51164794 None:intergenic 25.0%
! TTCCTAGAATTAGATGAAAA+GGG - Chr2:51165174-51165193 MsG0280009384.01.T01:intron 25.0%
! TTGATTGAACACTAAATTCA+GGG + Chr2:51165097-51165116 None:intergenic 25.0%
! TTTCCTAGAATTAGATGAAA+AGG - Chr2:51165173-51165192 MsG0280009384.01.T01:intron 25.0%
!! AAGTCAAAAATTGTAGCTTT+TGG - Chr2:51165052-51165071 MsG0280009384.01.T01:intron 25.0%
!!! AATTTTTGTTTCTCTATTGG+AGG - Chr2:51164933-51164952 MsG0280009384.01.T01:intron 25.0%
!!! ATGTTTGTTTTGATTAAGGA+GGG + Chr2:51165391-51165410 None:intergenic 25.0%
!!! GAATTGATTTTCAAGATTTC+TGG + Chr2:51165281-51165300 None:intergenic 25.0%
!!! GCAAATTTTTGTTTCTCTAT+TGG - Chr2:51164930-51164949 MsG0280009384.01.T01:intron 25.0%
!!! TAGTCCTTATCTTTTTTCTT+GGG - Chr2:51165856-51165875 MsG0280009384.01.T01:exon 25.0%
!!! TTAGTCCTTATCTTTTTTCT+TGG - Chr2:51165855-51165874 MsG0280009384.01.T01:exon 25.0%
AAAACCTTGGCTACAATAAT+TGG + Chr2:51164843-51164862 None:intergenic 30.0%
AAACATGTTAGTTCATTGAG+TGG - Chr2:51165405-51165424 MsG0280009384.01.T01:intron 30.0%
AAACATGTTTATTTCGGAGA+GGG + Chr2:51165793-51165812 None:intergenic 30.0%
AAACCTTGGCTACAATAATT+GGG + Chr2:51164842-51164861 None:intergenic 30.0%
AAATAAGTATCATGCATCCT+AGG + Chr2:51165319-51165338 None:intergenic 30.0%
AATGTATTATCCAGATGCAT+AGG - Chr2:51165541-51165560 MsG0280009384.01.T01:intron 30.0%
AGACAGACAGTGATATTAAA+TGG - Chr2:51164611-51164630 MsG0280009384.01.T01:three_prime_UTR 30.0%
AGATCACAATACCTCAAATT+AGG - Chr2:51164672-51164691 MsG0280009384.01.T01:exon 30.0%
GATCACAATACCTCAAATTA+GGG - Chr2:51164673-51164692 MsG0280009384.01.T01:exon 30.0%
GTTGATTGAACACTAAATTC+AGG + Chr2:51165098-51165117 None:intergenic 30.0%
TCATCTATGAAATGATTCCT+AGG - Chr2:51165299-51165318 MsG0280009384.01.T01:intron 30.0%
TCCTAGAATTAGATGAAAAG+GGG - Chr2:51165175-51165194 MsG0280009384.01.T01:intron 30.0%
TGATATTAAATGGCGTCTAT+TGG - Chr2:51164621-51164640 MsG0280009384.01.T01:three_prime_UTR 30.0%
TTAGTCATCAAATGTAGTGT+TGG - Chr2:51165215-51165234 MsG0280009384.01.T01:intron 30.0%
!! TGTGTTTTTCCTTGTTTGTT+GGG - Chr2:51165720-51165739 MsG0280009384.01.T01:intron 30.0%
!! TTGTGTTTTTCCTTGTTTGT+TGG - Chr2:51165719-51165738 MsG0280009384.01.T01:intron 30.0%
!!! CATGTTTGTTTTGATTAAGG+AGG + Chr2:51165392-51165411 None:intergenic 30.0%
!!! TGTTTGTTTTGATTAAGGAG+GGG + Chr2:51165390-51165409 None:intergenic 30.0%
CAAACATGTTTATTTCGGAG+AGG + Chr2:51165794-51165813 None:intergenic 35.0%
CAATGAAGGATCAGATGTAA+AGG - Chr2:51165642-51165661 MsG0280009384.01.T01:intron 35.0%
CCTAGAATTAGATGAAAAGG+GGG - Chr2:51165176-51165195 MsG0280009384.01.T01:intron 35.0%
CTTTACATCTGATCCTTCAT+TGG + Chr2:51165644-51165663 None:intergenic 35.0%
TTGAAGGTAACCCTAATTTG+AGG + Chr2:51164686-51164705 None:intergenic 35.0%
ACTTATCAACCACCCTACAA+CGG + Chr2:51165755-51165774 None:intergenic 40.0%
AGAGTCCAGCCAAAATTGTA+AGG + Chr2:51165699-51165718 None:intergenic 40.0%
GGATCAGATGTAAAGGAAAC+TGG - Chr2:51165649-51165668 MsG0280009384.01.T01:intron 40.0%
GGTGGAAAATCATCCAATGA+AGG - Chr2:51165628-51165647 MsG0280009384.01.T01:intron 40.0%
TGACCCAATTATTGTAGCCA+AGG - Chr2:51164836-51164855 MsG0280009384.01.T01:CDS 40.0%
! ACCTCCTCTCCTTACAATTT+TGG - Chr2:51165687-51165706 MsG0280009384.01.T01:intron 40.0%
! ATTGGATGATTTTCCACCAC+TGG + Chr2:51165626-51165645 None:intergenic 40.0%
! CCCCCTTTTCATCTAATTCT+AGG + Chr2:51165179-51165198 None:intergenic 40.0%
! TCCTTGTTTGTTGGGTCATT+GGG - Chr2:51165728-51165747 MsG0280009384.01.T01:intron 40.0%
! TTCCTTGTTTGTTGGGTCAT+TGG - Chr2:51165727-51165746 MsG0280009384.01.T01:intron 40.0%
!! ATTTCTAGAGCCTATGCATC+TGG + Chr2:51165554-51165573 None:intergenic 40.0%
!! GGAAGGAATTGAGGTTTTGA+AGG + Chr2:51164702-51164721 None:intergenic 40.0%
AATGCATGAACCAGACCTGA+AGG + Chr2:51164751-51164770 None:intergenic 45.0%
ACCCAATGACCCAACAAACA+AGG + Chr2:51165732-51165751 None:intergenic 45.0%
AGAGAAGTTGAGTGTGGCAA+TGG + Chr2:51164724-51164743 None:intergenic 45.0%
CCAGCCAAAATTGTAAGGAG+AGG + Chr2:51165694-51165713 None:intergenic 45.0%
GAAACTGGCAATGCTTCTCA+GGG - Chr2:51165664-51165683 MsG0280009384.01.T01:intron 45.0%
GAGAAGTTGAGTGTGGCAAT+GGG + Chr2:51164723-51164742 None:intergenic 45.0%
GCCAAAATTGTAAGGAGAGG+AGG + Chr2:51165691-51165710 None:intergenic 45.0%
TATTGTGATCTGACTGTCGC+CGG + Chr2:51164663-51164682 None:intergenic 45.0%
! ATTTTCCACCACTGGAGTTG+GGG + Chr2:51165618-51165637 None:intergenic 45.0%
! GATTTTCCACCACTGGAGTT+GGG + Chr2:51165619-51165638 None:intergenic 45.0%
! TGATTTTCCACCACTGGAGT+TGG + Chr2:51165620-51165639 None:intergenic 45.0%
!! CCTCTCCTTACAATTTTGGC+TGG - Chr2:51165691-51165710 MsG0280009384.01.T01:intron 45.0%
AGTTGAGTGTGGCAATGGGA+AGG + Chr2:51164719-51164738 None:intergenic 50.0%
CATGAACCAGACCTGAAGGT+GGG + Chr2:51164747-51164766 None:intergenic 50.0%
GGAAACTGGCAATGCTTCTC+AGG - Chr2:51165663-51165682 MsG0280009384.01.T01:intron 50.0%
GTGGCAATGGGAAGGAATTG+AGG + Chr2:51164711-51164730 None:intergenic 50.0%
TCAACTTCTCTCCCACCTTC+AGG - Chr2:51164733-51164752 MsG0280009384.01.T01:CDS 50.0%
TGGATTCTCCTCACTGTCAC+CGG - Chr2:51164641-51164660 MsG0280009384.01.T01:three_prime_UTR 50.0%
ACAAACCCCAACTCCAGTGG+TGG - Chr2:51165610-51165629 MsG0280009384.01.T01:intron 55.0%
GCATGAACCAGACCTGAAGG+TGG + Chr2:51164748-51164767 None:intergenic 55.0%
GCTACAAACCCCAACTCCAG+TGG - Chr2:51165607-51165626 MsG0280009384.01.T01:intron 55.0%
GGGTCATTGGGTCCGTTGTA+GGG - Chr2:51165740-51165759 MsG0280009384.01.T01:CDS 55.0%
GGTGGGAGAGAAGTTGAGTG+TGG + Chr2:51164730-51164749 None:intergenic 55.0%
TCATTGGGTCCGTTGTAGGG+TGG - Chr2:51165743-51165762 MsG0280009384.01.T01:CDS 55.0%
TGGGTCATTGGGTCCGTTGT+AGG - Chr2:51165739-51165758 MsG0280009384.01.T01:CDS 55.0%
TTCTCTCCCACCTTCAGGTC+TGG - Chr2:51164738-51164757 MsG0280009384.01.T01:CDS 55.0%
GACTGTCGCCGGTGACAGTG+AGG + Chr2:51164652-51164671 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 51164610 51165877 51164610 ID=MsG0280009384.01;Name=MsG0280009384.01
Chr2 mRNA 51164610 51165877 51164610 ID=MsG0280009384.01.T01;Parent=MsG0280009384.01;Name=MsG0280009384.01.T01;_AED=0.50;_eAED=0.50;_QI=20|1|1|1|0|0|2|73|124
Chr2 exon 51164610 51164932 51164610 ID=MsG0280009384.01.T01:exon:6538;Parent=MsG0280009384.01.T01
Chr2 exon 51165733 51165877 51165733 ID=MsG0280009384.01.T01:exon:6537;Parent=MsG0280009384.01.T01
Chr2 five_prime_UTR 51165858 51165877 51165858 ID=MsG0280009384.01.T01:five_prime_utr;Parent=MsG0280009384.01.T01
Chr2 CDS 51165733 51165857 51165733 ID=MsG0280009384.01.T01:cds;Parent=MsG0280009384.01.T01
Chr2 CDS 51164683 51164932 51164683 ID=MsG0280009384.01.T01:cds;Parent=MsG0280009384.01.T01
Chr2 three_prime_UTR 51164610 51164682 51164610 ID=MsG0280009384.01.T01:three_prime_utr;Parent=MsG0280009384.01.T01
Chr2 mRNA 51164683 51164955 51164683 ID=MsG0280009384.01.T02;Parent=MsG0280009384.01;Name=MsG0280009384.01.T02;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|90
Chr2 exon 51164683 51164955 51164683 ID=MsG0280009384.01.T02:exon:6539;Parent=MsG0280009384.01.T02
Chr2 CDS 51164683 51164955 51164683 ID=MsG0280009384.01.T02:cds;Parent=MsG0280009384.01.T02
Gene Sequence

>MsG0280009384.01.T02

ATGCTGTTGAATGTATTATCCAGATGCATAGGCTCTAGAAATTTGCAAATAAATCAAAAGCTATTGACTGTTTCTGCTACAAACCCCAACTCCAGTGGTGGAAAATCATCCAATGAAGGATCAGATGTAAAGGAAACTGGCAATGCTTCTCAGGGACCTCCTCTCCTTACAATTTTGGCTGGACTCTTTGTGTTTTTCCTTGTTTGTTGGGTCATTGGGTCCGTTGTAGGGTGGTTGATAAGTTTGATTGTTAATGCGCCCTCTCCGAAATAA

>MsG0280009384.01.T01

ATGGCGTCTATTGGATTCTCCTCACTGTCACCGGCGACAGTCAGATCACAATACCTCAAATTAGGGTTACCTTCAAAACCTCAATTCCTTCCCATTGCCACACTCAACTTCTCTCCCACCTTCAGATGCATAGGCTCTAGAAATTTGCAAATAAATCAAAAGCTATTGACTGTTTCTGCTACAAACCCCAACTCCAGTGGTGGAAAATCATCCAATGAAGGATCAGATGTAAAGGAAACTGGCAATGCTTCTCAGGGACCTCCTCTCCTTACAATTTTGGCTGGACTCTTTGTGTTTTTCCTTGTTTGTTGGGTCATTGGGTCCGTTGTAGGGTGGTTGATAAGTTTGATTGTTAATGCGCCCTCTCCGAAATAA

Protein sequence

>MsG0280009384.01.T02

MLLNVLSRCIGSRNLQINQKLLTVSATNPNSSGGKSSNEGSDVKETGNASQGPPLLTILAGLFVFFLVCWVIGSVVGWLISLIVNAPSPK*

>MsG0280009384.01.T01

MASIGFSSLSPATVRSQYLKLGLPSKPQFLPIATLNFSPTFRCIGSRNLQINQKLLTVSATNPNSSGGKSSNEGSDVKETGNASQGPPLLTILAGLFVFFLVCWVIGSVVGWLISLIVNAPSPK*