AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780038351.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038351.01.T01 MTR_7g052120 95.349 86 3 1 8 92 22 107 1.84e-57 172
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038351.01.T01 AT4G12970 64.557 79 23 2 16 91 26 102 2.11e-30 103

Find 45 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATATTATACCTTACTTTAT+TGG 0.123914 7:+44876723 MsG0780038351.01.T01:five_prime_UTR
GCAAAGAATGTTACAAATTT+AGG 0.126784 7:-44877028 None:intergenic
GATTCTACTTATTATTATAT+TGG 0.212378 7:+44876611 MsG0780038351.01.T01:five_prime_UTR
AGAATTCATTGGAACTTTAT+AGG 0.252743 7:-44876541 None:intergenic
TTGTAAAACTTATGACAATT+TGG 0.253093 7:-44876798 None:intergenic
TCTTTCAATTGTATATAGTT+TGG 0.295687 7:+44876639 MsG0780038351.01.T01:five_prime_UTR
GCATGAGTACTTTGGGAGTT+TGG 0.304026 7:-44876429 None:intergenic
AACTTTAGCTGCAAGAATTA+AGG 0.311122 7:-44876461 None:intergenic
GAAAATGAAACTTAACAAAT+AGG 0.322820 7:-44877594 None:intergenic
AAATTTGTAACATTCTTTGC+AGG 0.350016 7:+44877031 MsG0780038351.01.T01:CDS
AAATTAGTGCATGCTTAAAT+TGG 0.354366 7:-44876903 None:intergenic
TTCAAACTTAGTTCTCTTTG+AGG 0.370756 7:-44877091 None:intergenic
GTTGTCATTCAATTATAATA+AGG 0.379165 7:+44877566 MsG0780038351.01.T01:three_prime_UTR
CAAGAGTAGCATGAGTACTT+TGG 0.396531 7:-44876437 None:intergenic
TTCATTAAAATAGATTATAT+AGG 0.405160 7:+44877437 MsG0780038351.01.T01:three_prime_UTR
TTTGCAGGAATTGAAACAAA+AGG 0.418042 7:+44877046 MsG0780038351.01.T01:CDS
AAGAGTAGCATGAGTACTTT+GGG 0.420798 7:-44876436 None:intergenic
AATTCTAGAAGATTGATGAT+TGG 0.426618 7:+44877246 MsG0780038351.01.T01:CDS
CAAACACATCTGTAGTGATA+AGG 0.436257 7:-44877369 None:intergenic
TCAAAGAGAACTAAGTTTGA+AGG 0.442646 7:+44877093 MsG0780038351.01.T01:CDS
AGCACTTCTCTAAATTATCA+AGG 0.454527 7:+44876933 MsG0780038351.01.T01:five_prime_UTR
CTTGCAGCTAAAGTTATACA+AGG 0.476292 7:+44876469 MsG0780038351.01.T01:five_prime_UTR
AGAAGAAGGTGAGAATTCAT+TGG 0.483460 7:-44876552 None:intergenic
TAGTGATAAGGGCTGTTGAT+GGG 0.499193 7:-44877357 None:intergenic
TTGCAGGAATTGAAACAAAA+GGG 0.500483 7:+44877047 MsG0780038351.01.T01:CDS
AAACACATCTGTAGTGATAA+GGG 0.502259 7:-44877368 None:intergenic
ATGGGGTCATTTCCCTCCAC+AGG 0.509832 7:-44877339 None:intergenic
GAGTGTAAGGACTAAGAAGA+AGG 0.513859 7:-44876566 None:intergenic
GTAGTGATAAGGGCTGTTGA+TGG 0.515350 7:-44877358 None:intergenic
GTGCATGCTTAAATTGGTGG+TGG 0.523627 7:-44876897 None:intergenic
GAATCATAGTATCATTAAGT+AGG 0.529344 7:-44876511 None:intergenic
ACTAACAACTAATTAGAAGA+AGG 0.530841 7:-44877505 None:intergenic
TAGTTCTCTTTGAGGTTGTG+AGG 0.552834 7:-44877083 None:intergenic
TGCATGCTTAAATTGGTGGT+GGG 0.557157 7:-44876896 None:intergenic
CATTCATTGTAAGTACAAGT+TGG 0.566084 7:-44877279 None:intergenic
TTAGTGCATGCTTAAATTGG+TGG 0.573774 7:-44876900 None:intergenic
TTCAGGGTAGCAATGAAGCA+TGG 0.580977 7:+44877214 MsG0780038351.01.T01:intron
TTAAAACTTCAACAAAATCG+TGG 0.603191 7:-44876834 None:intergenic
AGCTGAGCAAGTTCCTGTGG+AGG 0.603576 7:+44877326 MsG0780038351.01.T01:CDS
GCTGAGCAAGTTCCTGTGGA+GGG 0.617680 7:+44877327 MsG0780038351.01.T01:CDS
CAGAGCTGAGCAAGTTCCTG+TGG 0.618590 7:+44877323 MsG0780038351.01.T01:CDS
CAGGAATTGAAACAAAAGGG+TGG 0.635486 7:+44877050 MsG0780038351.01.T01:CDS
AGTGATAAGGGCTGTTGATG+GGG 0.645976 7:-44877356 None:intergenic
TGTACTTACAATGAATGTAG+AGG 0.646526 7:+44877285 MsG0780038351.01.T01:CDS
GTAAGTACAAGTTGGTGCTG+TGG 0.653282 7:-44877271 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCATTAAAATAGATTATAT+AGG + Chr7:44877437-44877456 MsG0780038351.01.T01:three_prime_UTR 10.0%
!! GATTCTACTTATTATTATAT+TGG + Chr7:44876611-44876630 MsG0780038351.01.T01:five_prime_UTR 15.0%
!!! ATAATGTTAATTTTGTGTTT+TGG + Chr7:44877460-44877479 MsG0780038351.01.T01:three_prime_UTR 15.0%
!!! TAAGTTTTACAAAATTACAT+TGG + Chr7:44876809-44876828 MsG0780038351.01.T01:five_prime_UTR 15.0%
!!! TTTTGTATCTATTATTTTCA+GGG + Chr7:44877198-44877217 MsG0780038351.01.T01:intron 15.0%
!!! TTTTTGTATCTATTATTTTC+AGG + Chr7:44877197-44877216 MsG0780038351.01.T01:intron 15.0%
!! AAAAATACACCAATAAAGTA+AGG - Chr7:44876735-44876754 None:intergenic 20.0%
!! ATCAATTTGAATTAATTGCT+TGG + Chr7:44877136-44877155 MsG0780038351.01.T01:intron 20.0%
!! CATATTATACCTTACTTTAT+TGG + Chr7:44876723-44876742 MsG0780038351.01.T01:five_prime_UTR 20.0%
!! GTTGTCATTCAATTATAATA+AGG + Chr7:44877566-44877585 MsG0780038351.01.T01:three_prime_UTR 20.0%
!! TCTTTCAATTGTATATAGTT+TGG + Chr7:44876639-44876658 MsG0780038351.01.T01:five_prime_UTR 20.0%
!! TTGTAAAACTTATGACAATT+TGG - Chr7:44876801-44876820 None:intergenic 20.0%
! AAATTAGTGCATGCTTAAAT+TGG - Chr7:44876906-44876925 None:intergenic 25.0%
! AAATTTGTAACATTCTTTGC+AGG + Chr7:44877031-44877050 MsG0780038351.01.T01:CDS 25.0%
! ACAAAAAAAAAAGAGGAGAA+TGG + Chr7:44876400-44876419 MsG0780038351.01.T01:five_prime_UTR 25.0%
! ACTAACAACTAATTAGAAGA+AGG - Chr7:44877508-44877527 None:intergenic 25.0%
! AGAATTCATTGGAACTTTAT+AGG - Chr7:44876544-44876563 None:intergenic 25.0%
! CAAAAAAAAAAGAGGAGAAT+GGG + Chr7:44876401-44876420 MsG0780038351.01.T01:five_prime_UTR 25.0%
! GAATCATAGTATCATTAAGT+AGG - Chr7:44876514-44876533 None:intergenic 25.0%
! GCAAAGAATGTTACAAATTT+AGG - Chr7:44877031-44877050 None:intergenic 25.0%
! TTAAAACTTCAACAAAATCG+TGG - Chr7:44876837-44876856 None:intergenic 25.0%
!! AATTCTAGAAGATTGATGAT+TGG + Chr7:44877246-44877265 MsG0780038351.01.T01:CDS 25.0%
!! TGAAAAAAAGAAAGAGTGTA+AGG - Chr7:44876582-44876601 None:intergenic 25.0%
!!! TTTCTTTTTTTCACTTCTCT+TGG + Chr7:44876589-44876608 MsG0780038351.01.T01:five_prime_UTR 25.0%
AAACACATCTGTAGTGATAA+GGG - Chr7:44877371-44877390 None:intergenic 30.0%
AACTTTAGCTGCAAGAATTA+AGG - Chr7:44876464-44876483 None:intergenic 30.0%
AGCACTTCTCTAAATTATCA+AGG + Chr7:44876933-44876952 MsG0780038351.01.T01:five_prime_UTR 30.0%
CATTCATTGTAAGTACAAGT+TGG - Chr7:44877282-44877301 None:intergenic 30.0%
TCAAAGAGAACTAAGTTTGA+AGG + Chr7:44877093-44877112 MsG0780038351.01.T01:CDS 30.0%
TGTACTTACAATGAATGTAG+AGG + Chr7:44877285-44877304 MsG0780038351.01.T01:CDS 30.0%
TTGCAGGAATTGAAACAAAA+GGG + Chr7:44877047-44877066 MsG0780038351.01.T01:CDS 30.0%
TTTGCAGGAATTGAAACAAA+AGG + Chr7:44877046-44877065 MsG0780038351.01.T01:CDS 30.0%
! TTCAAACTTAGTTCTCTTTG+AGG - Chr7:44877094-44877113 None:intergenic 30.0%
AAGAGTAGCATGAGTACTTT+GGG - Chr7:44876439-44876458 None:intergenic 35.0%
AGAAGAAGGTGAGAATTCAT+TGG - Chr7:44876555-44876574 None:intergenic 35.0%
CAAACACATCTGTAGTGATA+AGG - Chr7:44877372-44877391 None:intergenic 35.0%
CTTGCAGCTAAAGTTATACA+AGG + Chr7:44876469-44876488 MsG0780038351.01.T01:five_prime_UTR 35.0%
TTAGTGCATGCTTAAATTGG+TGG - Chr7:44876903-44876922 None:intergenic 35.0%
CAAGAGTAGCATGAGTACTT+TGG - Chr7:44876440-44876459 None:intergenic 40.0%
CAGGAATTGAAACAAAAGGG+TGG + Chr7:44877050-44877069 MsG0780038351.01.T01:CDS 40.0%
GAGTGTAAGGACTAAGAAGA+AGG - Chr7:44876569-44876588 None:intergenic 40.0%
TAGTGATAAGGGCTGTTGAT+GGG - Chr7:44877360-44877379 None:intergenic 40.0%
! TGCATGCTTAAATTGGTGGT+GGG - Chr7:44876899-44876918 None:intergenic 40.0%
!! TAGTTCTCTTTGAGGTTGTG+AGG - Chr7:44877086-44877105 None:intergenic 40.0%
AGTGATAAGGGCTGTTGATG+GGG - Chr7:44877359-44877378 None:intergenic 45.0%
GTAGTGATAAGGGCTGTTGA+TGG - Chr7:44877361-44877380 None:intergenic 45.0%
GTGCATGCTTAAATTGGTGG+TGG - Chr7:44876900-44876919 None:intergenic 45.0%
TTCAGGGTAGCAATGAAGCA+TGG + Chr7:44877214-44877233 MsG0780038351.01.T01:intron 45.0%
! GCATGAGTACTTTGGGAGTT+TGG - Chr7:44876432-44876451 None:intergenic 45.0%
!! GTAAGTACAAGTTGGTGCTG+TGG - Chr7:44877274-44877293 None:intergenic 45.0%
AGCTGAGCAAGTTCCTGTGG+AGG + Chr7:44877326-44877345 MsG0780038351.01.T01:CDS 55.0%
ATGGGGTCATTTCCCTCCAC+AGG - Chr7:44877342-44877361 None:intergenic 55.0%
CAGAGCTGAGCAAGTTCCTG+TGG + Chr7:44877323-44877342 MsG0780038351.01.T01:CDS 55.0%
GCTGAGCAAGTTCCTGTGGA+GGG + Chr7:44877327-44877346 MsG0780038351.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr7 gene 44876400 44877601 44876400 ID=MsG0780038351.01;Name=MsG0780038351.01
Chr7 mRNA 44876400 44877601 44876400 ID=MsG0780038351.01.T01;Parent=MsG0780038351.01;Name=MsG0780038351.01.T01;_AED=0.44;_eAED=0.44;_QI=622|1|1|1|0|0|2|197|92
Chr7 exon 44876400 44877114 44876400 ID=MsG0780038351.01.T01:exon:4024;Parent=MsG0780038351.01.T01
Chr7 exon 44877219 44877601 44877219 ID=MsG0780038351.01.T01:exon:4025;Parent=MsG0780038351.01.T01
Chr7 five_prime_UTR 44876400 44877021 44876400 ID=MsG0780038351.01.T01:five_prime_utr;Parent=MsG0780038351.01.T01
Chr7 CDS 44877022 44877114 44877022 ID=MsG0780038351.01.T01:cds;Parent=MsG0780038351.01.T01
Chr7 CDS 44877219 44877404 44877219 ID=MsG0780038351.01.T01:cds;Parent=MsG0780038351.01.T01
Chr7 three_prime_UTR 44877405 44877601 44877405 ID=MsG0780038351.01.T01:three_prime_utr;Parent=MsG0780038351.01.T01
Gene Sequence

>MsG0780038351.01.T01

ATGTATCCTAAATTTGTAACATTCTTTGCAGGAATTGAAACAAAAGGGTGGATATCACAATCCTCACAACCTCAAAGAGAACTAAGTTTGAAGGGTAGCAATGAAGCATGGAAACAAAGAAATTCTAGAAGATTGATGATTGGATCCACAGCACCAACTTGTACTTACAATGAATGTAGAGGCTGCAAATATAGATGCAGAGCTGAGCAAGTTCCTGTGGAGGGAAATGACCCCATCAACAGCCCTTATCACTACAGATGTGTTTGTCATAGATCATGA

Protein sequence

>MsG0780038351.01.T01

MYPKFVTFFAGIETKGWISQSSQPQRELSLKGSNEAWKQRNSRRLMIGSTAPTCTYNECRGCKYRCRAEQVPVEGNDPINSPYHYRCVCHRS*