AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023909.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023909.01.T01 MTR_8g104740 98.182 55 1 0 41 95 399 453 3.67e-31 114
MsG0480023909.01.T01 MTR_4g120810 100.000 55 0 0 41 95 609 663 5.26e-31 114
MsG0480023909.01.T01 MTR_8g104740 98.182 55 1 0 41 95 611 665 1.98e-30 112
MsG0480023909.01.T01 MTR_4g052070 96.364 55 2 0 41 95 589 643 5.86e-30 111
MsG0480023909.01.T01 MTR_1g113700 85.484 62 2 1 41 95 548 609 1.81e-27 104
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023909.01.T01 AT4G33760 83.636 55 9 0 41 95 473 527 6.46e-27 102
MsG0480023909.01.T01 AT4G33760 83.636 55 9 0 41 95 609 663 8.52e-27 102

Find 23 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCAGGCTGAAGCTAAGTT+TGG 0.318198 4:-91145099 MsG0480023909.01.T01:CDS
TGAAAGATCTTTCAGCTGTT+GGG 0.361402 4:+91144209 None:intergenic
AATGCAGGAGGCATAGCTTT+TGG 0.364469 4:-91144348 MsG0480023909.01.T01:CDS
AGATTGGTTATGTTGCTAGC+TGG 0.406190 4:-91144318 MsG0480023909.01.T01:CDS
GATTGGTTATGTTGCTAGCT+GGG 0.406860 4:-91144317 MsG0480023909.01.T01:CDS
TTGAAAGATCTTTCAGCTGT+TGG 0.431611 4:+91144208 None:intergenic
TAGCTGGGGCTAATTCAATC+AGG 0.434319 4:-91144302 MsG0480023909.01.T01:CDS
ATTATTTATATATCAAATGC+AGG 0.486124 4:-91144363 MsG0480023909.01.T01:CDS
GAAAGATCTTTCAGCTGTTG+GGG 0.499570 4:+91144210 None:intergenic
TTCTTGAAGCTCTTGACATG+GGG 0.505034 4:-91145071 MsG0480023909.01.T01:CDS
CTTCTTGAAGCTCTTGACAT+GGG 0.540430 4:-91145072 MsG0480023909.01.T01:CDS
CAGCTGTTGGGGATCCACCT+CGG 0.573724 4:+91144221 None:intergenic
TCTTCTTGAAGCTCTTGACA+TGG 0.595100 4:-91145073 MsG0480023909.01.T01:CDS
CCTCGGAAGGTGATCTCGTG+AGG 0.598148 4:+91144238 None:intergenic
TGTTGGGGATCCACCTCGGA+AGG 0.606271 4:+91144225 None:intergenic
ATTGGTTATGTTGCTAGCTG+GGG 0.606846 4:-91144316 MsG0480023909.01.T01:CDS
CACGAGATCACCTTCCGAGG+TGG 0.609144 4:-91144235 MsG0480023909.01.T01:CDS
AGCTGGGGCTAATTCAATCA+GGG 0.612567 4:-91144301 MsG0480023909.01.T01:CDS
TCTTGAAGCTCTTGACATGG+GGG 0.620703 4:-91145070 MsG0480023909.01.T01:CDS
CTCGGAAGGTGATCTCGTGA+GGG 0.643918 4:+91144239 None:intergenic
TTAGAAGACTTACCATGTGG+CGG 0.647061 4:+91145045 None:intergenic
CCTCACGAGATCACCTTCCG+AGG 0.653984 4:-91144238 MsG0480023909.01.T01:CDS
ATTTATATATCAAATGCAGG+AGG 0.679862 4:-91144360 MsG0480023909.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATTTATATATCAAATGC+AGG - Chr4:91144950-91144969 MsG0480023909.01.T01:intron 15.0%
!!! TTATCAAGTTGAAATTTAAT+TGG - Chr4:91144838-91144857 MsG0480023909.01.T01:intron 15.0%
!! CTTCAATAAATAAAGAAAAC+TGG + Chr4:91144393-91144412 None:intergenic 20.0%
!!! TGCATTATAATTTCTTTTTC+AGG - Chr4:91144198-91144217 MsG0480023909.01.T01:CDS 20.0%
! ATTTATATATCAAATGCAGG+AGG - Chr4:91144953-91144972 MsG0480023909.01.T01:intron 25.0%
!! AGTTCAAGCAATTTTATTGA+AGG - Chr4:91144670-91144689 MsG0480023909.01.T01:intron 25.0%
!! TGAAATGTTTTCAGTTTAGT+GGG + Chr4:91144801-91144820 None:intergenic 25.0%
!! TTGAAATGTTTTCAGTTTAG+TGG + Chr4:91144802-91144821 None:intergenic 25.0%
!!! ATTTGCTATTATCACTAATG+CGG - Chr4:91144524-91144543 MsG0480023909.01.T01:intron 25.0%
AATGATCACAACACAATCAA+TGG - Chr4:91144500-91144519 MsG0480023909.01.T01:intron 30.0%
ACTTAGTGAATAGAATGATG+TGG - Chr4:91144700-91144719 MsG0480023909.01.T01:intron 30.0%
ATAATCCAGTTTATCGACAA+GGG + Chr4:91144316-91144335 None:intergenic 30.0%
TGATCCTGAAAACATGTATA+TGG - Chr4:91144564-91144583 MsG0480023909.01.T01:intron 30.0%
! ATTGCCATATACATGTTTTC+AGG + Chr4:91144571-91144590 None:intergenic 30.0%
!! ATTTTAGAAGACTTACCATG+TGG + Chr4:91144274-91144293 None:intergenic 30.0%
!! CTGTAGCTTTAACATAAGTT+TGG + Chr4:91144633-91144652 None:intergenic 30.0%
!! TTTGCTATTATCACTAATGC+GGG - Chr4:91144525-91144544 MsG0480023909.01.T01:intron 30.0%
AGCATTTATCACTAACTGCA+AGG - Chr4:91144414-91144433 MsG0480023909.01.T01:intron 35.0%
GATAATCCAGTTTATCGACA+AGG + Chr4:91144317-91144336 None:intergenic 35.0%
TGAAAGATCTTTCAGCTGTT+GGG + Chr4:91145107-91145126 None:intergenic 35.0%
TTGAAAGATCTTTCAGCTGT+TGG + Chr4:91145108-91145127 None:intergenic 35.0%
! AGCACTACTCTAAAAACATG+AGG - Chr4:91144875-91144894 MsG0480023909.01.T01:intron 35.0%
! TGTTTTCAGTTTAGTGGGAA+TGG + Chr4:91144796-91144815 None:intergenic 35.0%
!!! ACTGTGCAGTTGTTGTTTTT+GGG + Chr4:91145051-91145070 None:intergenic 35.0%
ATTGGTTATGTTGCTAGCTG+GGG - Chr4:91144997-91145016 MsG0480023909.01.T01:intron 40.0%
CATATCGAGGAAAGGGAAAA+AGG - Chr4:91144367-91144386 MsG0480023909.01.T01:CDS 40.0%
CTTCTTGAAGCTCTTGACAT+GGG - Chr4:91144241-91144260 MsG0480023909.01.T01:CDS 40.0%
GAAAGATCTTTCAGCTGTTG+GGG + Chr4:91145106-91145125 None:intergenic 40.0%
TATCACTAACTGCAAGGTAG+TGG - Chr4:91144420-91144439 MsG0480023909.01.T01:intron 40.0%
TCTTCTTGAAGCTCTTGACA+TGG - Chr4:91144240-91144259 MsG0480023909.01.T01:CDS 40.0%
TTAGAAGACTTACCATGTGG+CGG + Chr4:91144271-91144290 None:intergenic 40.0%
TTCTTGAAGCTCTTGACATG+GGG - Chr4:91144242-91144261 MsG0480023909.01.T01:CDS 40.0%
TTTATCGACAAGGGAGGAAT+GGG + Chr4:91144307-91144326 None:intergenic 40.0%
TTTCAGGCTGAAGCTAAGTT+TGG - Chr4:91144214-91144233 MsG0480023909.01.T01:CDS 40.0%
! AGATTGGTTATGTTGCTAGC+TGG - Chr4:91144995-91145014 MsG0480023909.01.T01:intron 40.0%
! CTTTTTCCCTTTCCTCGATA+TGG + Chr4:91144369-91144388 None:intergenic 40.0%
! GATTGGTTATGTTGCTAGCT+GGG - Chr4:91144996-91145015 MsG0480023909.01.T01:intron 40.0%
!!! CACTGTGCAGTTGTTGTTTT+TGG + Chr4:91145052-91145071 None:intergenic 40.0%
AGCTGGGGCTAATTCAATCA+GGG - Chr4:91145012-91145031 MsG0480023909.01.T01:intron 45.0%
ATCCAGTTTATCGACAAGGG+AGG + Chr4:91144313-91144332 None:intergenic 45.0%
GCACAACCATATCGAGGAAA+GGG - Chr4:91144360-91144379 MsG0480023909.01.T01:CDS 45.0%
GTTTATCGACAAGGGAGGAA+TGG + Chr4:91144308-91144327 None:intergenic 45.0%
TAGCTGGGGCTAATTCAATC+AGG - Chr4:91145011-91145030 MsG0480023909.01.T01:intron 45.0%
TCAGGATCAGCGTTAATACG+TGG + Chr4:91144553-91144572 None:intergenic 45.0%
TCTTGAAGCTCTTGACATGG+GGG - Chr4:91144243-91144262 MsG0480023909.01.T01:CDS 45.0%
TGCACAACCATATCGAGGAA+AGG - Chr4:91144359-91144378 MsG0480023909.01.T01:CDS 45.0%
TTATCGACAAGGGAGGAATG+GGG + Chr4:91144306-91144325 None:intergenic 45.0%
TTCCTCCCTTGTCGATAAAC+TGG - Chr4:91144308-91144327 MsG0480023909.01.T01:CDS 45.0%
! AGCTTTTGGTCTGGACAGAT+TGG - Chr4:91144979-91144998 MsG0480023909.01.T01:intron 45.0%
! AGGTAGTGGCAGTTTAGACA+AGG - Chr4:91144434-91144453 MsG0480023909.01.T01:intron 45.0%
! GGTAGTGGCAGTTTAGACAA+GGG - Chr4:91144435-91144454 MsG0480023909.01.T01:intron 45.0%
!! AATGCAGGAGGCATAGCTTT+TGG - Chr4:91144965-91144984 MsG0480023909.01.T01:intron 45.0%
AAAGCTACAGCTGCAGAGAC+AGG - Chr4:91144643-91144662 MsG0480023909.01.T01:intron 50.0%
GTCTGTGCACAACCATATCG+AGG - Chr4:91144354-91144373 MsG0480023909.01.T01:CDS 50.0%
!! AGGAGGCATAGCTTTTGGTC+TGG - Chr4:91144970-91144989 MsG0480023909.01.T01:intron 50.0%
CTCGGAAGGTGATCTCGTGA+GGG + Chr4:91145077-91145096 None:intergenic 55.0%
CACGAGATCACCTTCCGAGG+TGG - Chr4:91145078-91145097 MsG0480023909.01.T01:CDS 60.0%
CAGCTGTTGGGGATCCACCT+CGG + Chr4:91145095-91145114 None:intergenic 60.0%
CCTCACGAGATCACCTTCCG+AGG - Chr4:91145075-91145094 MsG0480023909.01.T01:CDS 60.0%
CCTCGGAAGGTGATCTCGTG+AGG + Chr4:91145078-91145097 None:intergenic 60.0%
TGTTGGGGATCCACCTCGGA+AGG + Chr4:91145091-91145110 None:intergenic 60.0%
GACATGGGGGCTCCGCCACA+TGG - Chr4:91144256-91144275 MsG0480023909.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr4 gene 91144197 91145138 91144197 ID=MsG0480023909.01;Name=MsG0480023909.01
Chr4 mRNA 91144197 91145138 91144197 ID=MsG0480023909.01.T01;Parent=MsG0480023909.01;Name=MsG0480023909.01.T01;_AED=0.49;_eAED=0.60;_QI=0|0|0|1|0|0.5|2|0|95
Chr4 exon 91145058 91145138 91145058 ID=MsG0480023909.01.T01:exon:5129;Parent=MsG0480023909.01.T01
Chr4 exon 91144197 91144403 91144197 ID=MsG0480023909.01.T01:exon:5128;Parent=MsG0480023909.01.T01
Chr4 CDS 91145058 91145138 91145058 ID=MsG0480023909.01.T01:cds;Parent=MsG0480023909.01.T01
Chr4 CDS 91144197 91144403 91144197 ID=MsG0480023909.01.T01:cds;Parent=MsG0480023909.01.T01
Gene Sequence

>MsG0480023909.01.T01

ATGCATTATAATTTCTTTTTCAGGCTGAAGCTAAGTTTGGATATCTTCTTGAAGCTCTTGACATGGGGGCTCCGCCACATGTTGATTTTATCGATGATTATTATTTATATATCAAATGCAGGAGGCATAGCTTTTGGTCTGGACAGATTGGTTATGTTGCTAGCTGGGGCTAATTCAATCAGGGATGTCATTGCTTTCCCAAAAACAACAACTGCACAGTGTGCCCTCACGAGATCACCTTCCGAGGTGGATCCCCAACAGCTGAAAGATCTTTCAATCACTACATAG

Protein sequence

>MsG0480023909.01.T01

MHYNFFFRLKLSLDIFLKLLTWGLRHMLILSMIIIYISNAGGIAFGLDRLVMLLAGANSIRDVIAFPKTTTAQCALTRSPSEVDPQQLKDLSITT*