AlfalfaGEDB | Msa0005430

M. sativa cultivar zhongmu-4 / Msa0005430

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0005420	Msa0005430	0.967099	8.187592e-127	-8.615850e-47
Msa0005430	Msa0099800	0.856564	2.953363e-62	-8.615850e-47
Msa0005430	Msa0189320	0.804795	1.820122e-49	-8.615850e-47
Msa0005430	Msa0309770	0.810688	1.017781e-50	-8.615850e-47
Msa0005430	Msa0577500	0.803593	3.236765e-49	-8.615850e-47
Msa0005430	Msa0602390	0.814681	1.361460e-51	-8.615850e-47
Msa0005430	Msa0607050	0.811997	5.289796e-51	-8.615850e-47
Msa0005430	Msa0663390	0.803614	3.204433e-49	-8.615850e-47
Msa0005430	Msa0688960	0.804079	2.565730e-49	-8.615850e-47
Msa0005430	Msa0709400	0.808755	2.650214e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0005430	MtrunA17_Chr1g0150441	98.438	64	1	0	1	64	1	64	2.14e-40	126

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0005430	AT5G10745.1	66.667	39	13	9	47	8	46	3.49e-12	55.1
Msa0005430	AT5G10745.3	66.667	39	13	9	47	8	46	3.49e-12	55.1
Msa0005430	AT5G10745.2	66.667	39	13	9	47	8	46	3.49e-12	55.1

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Find 30 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGATCCCTCTCTGTTGCT+CGG	0.569041	1_1:-8012480	None:intergenic
AAAGCAACATCCCAAGATAC+TGG	0.517738	1_1:-8015081	None:intergenic
AACATCCCAAGATACTGGTC+TGG	0.525580	1_1:-8015076	None:intergenic
AATTGAAACGGTGAAGGATG+TGG	0.561795	1_1:-8012554	None:intergenic
ACAACAGCAACAGAGGAGAA+AGG	0.567130	1_1:+8012848	Msa0005430:CDS
ACACACACTCTGAATTATAT+GGG	0.523466	1_1:-8015436	None:intergenic
AGTATCTTGGGATGTTGCTT+TGG	0.408343	1_1:+8015083	Msa0005430:three_prime_UTR
CACTAGAGCAATCTCGTCCA+TGG	0.558619	1_1:+8012599	Msa0005430:CDS
CACTCTGAATTATATGGGTC+TGG	0.418705	1_1:-8015431	None:intergenic
CAGGTATTATGTTAGATCAT+TGG	0.345785	1_1:+8015018	Msa0005430:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTATGTTAAATGGTGTGCT+AGG	+	chr1_1:8013626-8013645	Msa0005430:intron	30.0%
TTATGTTAAATGGTGTGCTA+GGG	+	chr1_1:8013627-8013646	Msa0005430:intron	30.0%
GATCATGTAGAAGACAAATT+TGG	+	chr1_1:8013671-8013690	Msa0005430:intron	30.0%
TATTGTTGAAACTGAAAAGG+AGG	+	chr1_1:8013711-8013730	Msa0005430:intron	30.0%
GACAAAAACAAATATTGCAC+AGG	-	chr1_1:8013767-8013786	None:intergenic	30.0%
TTTGGTAAGGTCATATTTAG+AGG	+	chr1_1:8013885-8013904	Msa0005430:intron	30.0%
AAACCAATAAGGAGAACAAA+GGG	-	chr1_1:8014029-8014048	None:intergenic	30.0%
AAAACCAATAAGGAGAACAA+AGG	-	chr1_1:8014030-8014049	None:intergenic	30.0%
GTGAAAAGAGAAAACCAATA+AGG	-	chr1_1:8014040-8014059	None:intergenic	30.0%
CTTTCTTCTATAATATGCCT+AGG	-	chr1_1:8014172-8014191	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	8012466	8015582	8012466	ID=Msa0005430;Name=Msa0005430
chr1_1	mRNA	8012466	8015582	8012466	ID=Msa0005430-mRNA-1;Parent=Msa0005430;Name=Msa0005430-mRNA-1;_AED=0.26;_eAED=0.26;_QI=123\|1\|1\|1\|0.33\|0.25\|4\|276\|64
chr1_1	exon	8012466	8012681	8012466	ID=Msa0005430-mRNA-1:exon:2824;Parent=Msa0005430-mRNA-1
chr1_1	exon	8012777	8012898	8012777	ID=Msa0005430-mRNA-1:exon:2825;Parent=Msa0005430-mRNA-1
chr1_1	exon	8015021	8015104	8015021	ID=Msa0005430-mRNA-1:exon:2826;Parent=Msa0005430-mRNA-1
chr1_1	exon	8015411	8015582	8015411	ID=Msa0005430-mRNA-1:exon:2827;Parent=Msa0005430-mRNA-1
chr1_1	five_prime_UTR	8012466	8012588	8012466	ID=Msa0005430-mRNA-1:five_prime_utr;Parent=Msa0005430-mRNA-1
chr1_1	CDS	8012589	8012681	8012589	ID=Msa0005430-mRNA-1:cds;Parent=Msa0005430-mRNA-1
chr1_1	CDS	8012777	8012878	8012777	ID=Msa0005430-mRNA-1:cds;Parent=Msa0005430-mRNA-1
chr1_1	three_prime_UTR	8012879	8012898	8012879	ID=Msa0005430-mRNA-1:three_prime_utr;Parent=Msa0005430-mRNA-1
chr1_1	three_prime_UTR	8015021	8015104	8015021	ID=Msa0005430-mRNA-1:three_prime_utr;Parent=Msa0005430-mRNA-1
chr1_1	three_prime_UTR	8015411	8015582	8015411	ID=Msa0005430-mRNA-1:three_prime_utr;Parent=Msa0005430-mRNA-1

Gene Sequence	>Msa0005430 ATGGCGAATTCACTAGAGCAATCTCGTCCATGGTTCCTCTACGCAGTTCCTTTGTTGGTTTTTCTTTTGATCGCATTTCATGTCCTCGCTTTGGTGTATTGGATTTATAGATTGTCTACTGATACGAAGCCACAACAACAGACACTACAACAACAGCTTCAGCAACAACAGCAACAGAGGAGAAAGGCTCACTGA
Protein sequence	>Msa0005430 MANSLEQSRPWFLYAVPLLVFLLIAFHVLALVYWIYRLSTDTKPQQQTLQQQLQQQQQQRRKAH*