AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0577500

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0577500 MtrunA17_Chr8g0377671 97.909 287 6 0 1 287 1 287 0.0 552
Msa0577500 MtrunA17_Chr8g0377191 71.642 201 57 0 13 213 5 205 2.96e-97 284
Msa0577500 MtrunA17_Chr8g0377191 56.329 158 69 0 113 270 13 170 9.88e-53 171
Msa0577500 MtrunA17_Chr8g0377191 50.000 114 57 0 156 269 10 123 8.68e-29 109
Msa0577500 MtrunA17_Chr8g0385241 46.471 340 102 3 12 271 2 341 3.12e-84 259
Msa0577500 MtrunA17_Chr8g0385241 38.889 162 75 2 21 158 182 343 1.27e-22 96.7
Msa0577500 MtrunA17_Chr4g0059911 51.737 259 123 2 15 271 4 262 3.07e-80 247
Msa0577500 MtrunA17_Chr2g0316631 43.561 264 149 0 15 278 31 294 1.97e-68 214
Msa0577500 MtrunA17_Chr2g0316631 39.000 200 122 0 20 219 105 304 8.90e-44 151
Msa0577500 MtrunA17_Chr8g0382971 41.916 334 132 4 15 286 20 353 1.98e-68 216
Msa0577500 MtrunA17_Chr8g0377211 69.481 154 46 1 134 287 19 171 2.21e-68 209
Msa0577500 MtrunA17_Chr8g0377211 58.192 177 50 2 1 177 1 153 3.83e-60 189
Msa0577500 MtrunA17_Chr2g0316611 42.412 257 148 0 15 271 31 287 6.71e-67 213
Msa0577500 MtrunA17_Chr2g0316611 40.873 252 149 0 20 271 105 356 4.28e-59 192
Msa0577500 MtrunA17_Chr2g0316611 39.303 201 122 0 19 219 173 373 1.05e-41 147
Msa0577500 MtrunA17_Chr8g0380791 53.646 192 89 0 8 199 1 192 4.03e-64 214
Msa0577500 MtrunA17_Chr8g0380791 40.252 159 95 0 111 269 12 170 1.14e-31 124
Msa0577500 MtrunA17_Chr8g0380791 38.938 113 69 0 156 268 11 123 1.75e-15 76.6
Msa0577500 MtrunA17_Chr6g0467061 44.130 247 138 0 20 266 88 334 2.40e-62 200
Msa0577500 MtrunA17_Chr6g0467061 38.384 297 133 3 14 283 13 286 2.87e-57 187
Msa0577500 MtrunA17_Chr6g0467061 43.575 179 101 0 20 198 157 335 8.19e-39 139
Msa0577500 MtrunA17_Chr8g0383331 62.195 164 61 1 109 271 1 164 4.32e-62 193
Msa0577500 MtrunA17_Chr8g0383331 58.974 156 63 1 63 217 1 156 4.65e-57 181
Msa0577500 MtrunA17_Chr8g0383331 53.741 147 68 0 20 166 4 150 1.20e-46 154
Msa0577500 MtrunA17_Chr8g0383551 41.901 284 104 7 13 264 20 274 1.23e-54 181
Msa0577500 MtrunA17_Chr8g0383551 34.513 226 91 5 85 269 24 233 2.48e-26 106
Msa0577500 MtrunA17_Chr5g0424111 43.094 181 103 0 14 194 191 371 3.53e-40 145
Msa0577500 MtrunA17_Chr5g0424111 39.560 182 103 1 95 269 173 354 1.26e-34 130
Msa0577500 MtrunA17_Chr5g0424111 37.853 177 110 0 65 241 196 372 1.10e-30 119
Msa0577500 MtrunA17_Chr8g0377221 34.934 229 137 3 18 244 3 221 1.00e-39 139
Msa0577500 MtrunA17_Chr8g0377221 37.288 118 74 0 154 271 1 118 4.83e-19 84.7
Msa0577500 MtrunA17_Chr8g0377221 29.101 189 119 2 89 262 5 193 3.01e-17 79.7
Msa0577500 MtrunA17_Chr8g0353121 39.024 205 123 1 8 210 39 243 1.07e-39 147
Msa0577500 MtrunA17_Chr8g0353121 35.426 223 138 3 62 281 51 270 2.32e-30 120
Msa0577500 MtrunA17_Chr1g0180261 40.323 186 111 0 14 199 45 230 1.42e-37 141
Msa0577500 MtrunA17_Chr1g0180261 38.798 183 112 0 63 245 48 230 2.57e-30 120
Msa0577500 MtrunA17_Chr1g0180261 36.250 160 102 0 109 268 48 207 4.00e-25 105
Msa0577500 MtrunA17_Chr1g0180261 30.657 137 95 0 134 270 50 186 1.75e-13 70.5
Msa0577500 MtrunA17_Chr3g0082501 37.255 204 118 1 16 209 60 263 3.74e-37 139
Msa0577500 MtrunA17_Chr3g0082501 34.597 211 137 1 67 277 65 274 5.65e-31 122
Msa0577500 MtrunA17_Chr5g0424301 40.113 177 106 0 7 183 56 232 5.73e-37 139
Msa0577500 MtrunA17_Chr5g0424301 35.220 159 103 0 112 270 69 227 1.92e-25 105
Msa0577500 MtrunA17_Chr5g0424301 32.984 191 122 2 39 229 48 232 5.98e-25 104
Msa0577500 MtrunA17_Chr5g0424301 37.681 138 86 0 134 271 68 205 3.48e-22 96.3
Msa0577500 MtrunA17_Chr1g0171491 40.217 184 110 0 16 199 57 240 5.35e-36 136
Msa0577500 MtrunA17_Chr1g0171491 32.456 228 147 2 67 287 62 289 4.62e-30 119
Msa0577500 MtrunA17_Chr1g0171491 36.970 165 102 1 104 268 55 217 1.96e-26 108
Msa0577500 MtrunA17_Chr1g0171491 34.975 203 130 1 27 229 47 247 4.43e-26 107
Msa0577500 MtrunA17_Chr7g0233351 40.556 180 107 0 17 196 189 368 3.73e-35 131
Msa0577500 MtrunA17_Chr7g0233351 39.884 173 104 0 44 216 193 365 6.64e-32 122
Msa0577500 MtrunA17_Chr7g0233351 35.294 187 121 0 62 248 188 374 1.28e-31 122
Msa0577500 MtrunA17_Chr7g0233351 39.506 162 98 0 108 269 188 349 7.57e-30 117
Msa0577500 MtrunA17_Chr7g0233351 36.571 175 111 0 90 264 193 367 5.16e-28 112
Msa0577500 MtrunA17_Chr8g0336161 39.891 183 110 0 14 196 191 373 6.91e-34 128
Msa0577500 MtrunA17_Chr8g0336161 36.957 184 116 0 65 248 196 379 1.22e-30 119
Msa0577500 MtrunA17_Chr8g0336161 39.506 162 98 0 108 269 193 354 8.13e-28 111
Msa0577500 MtrunA17_Chr3g0114791 39.560 182 108 1 14 193 177 358 7.23e-34 127
Msa0577500 MtrunA17_Chr3g0114791 35.326 184 119 0 53 236 172 355 1.97e-30 118
Msa0577500 MtrunA17_Chr3g0114791 37.423 163 102 0 106 268 179 341 2.80e-29 115
Msa0577500 MtrunA17_Chr3g0114791 32.500 200 132 1 84 283 180 376 1.29e-25 105
Msa0577500 MtrunA17_Chr3g0114791 37.391 115 72 0 148 262 175 289 7.66e-16 77.0
Msa0577500 MtrunA17_Chr3g0115111 37.158 183 114 1 8 189 45 227 2.05e-32 126
Msa0577500 MtrunA17_Chr3g0115111 32.653 196 129 2 76 270 23 216 6.30e-27 110
Msa0577500 MtrunA17_Chr3g0115111 33.032 221 138 4 39 258 35 246 1.63e-26 108
Msa0577500 MtrunA17_Chr3g0115111 36.471 170 102 2 21 189 82 246 1.77e-22 97.1
Msa0577500 MtrunA17_Chr3g0115111 35.971 139 88 1 134 271 56 194 7.57e-20 89.4
Msa0577500 MtrunA17_Chr4g0055371 39.205 176 107 0 41 216 170 345 3.17e-32 123
Msa0577500 MtrunA17_Chr4g0055371 38.889 162 99 0 108 269 168 329 8.17e-28 111
Msa0577500 MtrunA17_Chr4g0055371 33.514 185 123 0 58 242 164 348 3.71e-27 109
Msa0577500 MtrunA17_Chr4g0055371 35.227 176 114 0 89 264 172 347 6.12e-26 105
Msa0577500 MtrunA17_Chr7g0256301 40.113 177 106 0 14 190 65 241 6.09e-32 124
Msa0577500 MtrunA17_Chr7g0256301 38.217 157 97 0 112 268 71 227 3.76e-24 102
Msa0577500 MtrunA17_Chr7g0256301 35.673 171 110 0 66 236 71 241 2.33e-23 99.8
Msa0577500 MtrunA17_Chr7g0256301 33.918 171 113 0 89 259 71 241 1.39e-21 94.7
Msa0577500 MtrunA17_Chr8g0377201 64.368 87 31 0 201 287 1 87 1.74e-31 112
Msa0577500 MtrunA17_Chr8g0377201 54.545 66 30 0 63 128 1 66 4.17e-17 74.7
Msa0577500 MtrunA17_Chr8g0377201 59.677 62 25 0 109 170 1 62 2.83e-16 72.4
Msa0577500 MtrunA17_Chr8g0377201 68.889 45 14 0 19 63 26 70 2.95e-13 64.3
Msa0577500 MtrunA17_Chr8g0377201 43.284 67 38 0 89 155 4 70 3.40e-11 58.5
Msa0577500 MtrunA17_Chr3g0131001 41.714 175 102 0 16 190 51 225 2.27e-31 123
Msa0577500 MtrunA17_Chr3g0131001 36.723 177 112 0 64 240 53 229 4.28e-21 93.2
Msa0577500 MtrunA17_Chr3g0131001 34.503 171 112 0 89 259 55 225 2.36e-19 87.8
Msa0577500 MtrunA17_Chr3g0131001 37.107 159 100 0 112 270 55 213 6.27e-19 86.7
Msa0577500 MtrunA17_Chr3g0131001 38.406 138 85 0 134 271 54 191 2.73e-18 84.7
Msa0577500 MtrunA17_Chr8g0385201 37.158 183 113 2 14 195 24 205 5.24e-29 116
Msa0577500 MtrunA17_Chr8g0385201 38.125 160 98 1 112 270 30 189 4.29e-27 110
Msa0577500 MtrunA17_Chr8g0385201 36.041 197 120 4 67 258 31 226 2.74e-25 105
Msa0577500 MtrunA17_Chr5g0398011 46.903 113 60 0 18 130 33 145 8.50e-27 102
Msa0577500 MtrunA17_Chr5g0398011 37.607 117 73 0 155 271 32 148 1.78e-20 85.5
Msa0577500 MtrunA17_Chr5g0398011 29.655 145 96 1 32 176 7 145 2.81e-13 66.2
Msa0577500 MtrunA17_Chr5g0398011 32.456 114 77 0 109 222 32 145 3.44e-13 65.9
Msa0577500 MtrunA17_Chr5g0398021 45.192 104 57 0 20 123 13 116 7.10e-24 94.7
Msa0577500 MtrunA17_Chr5g0398021 35.338 133 86 0 155 287 10 142 4.04e-20 84.7
Msa0577500 MtrunA17_Chr5g0398021 33.621 116 77 0 102 217 3 118 6.55e-16 73.6
Msa0577500 MtrunA17_Chr5g0398021 37.383 107 67 0 63 169 10 116 2.74e-15 71.6
Msa0577500 MtrunA17_Chr5g0398021 29.730 111 78 0 81 191 5 115 4.03e-14 68.6
Msa0577500 MtrunA17_Chr3g0085921 63.333 60 22 0 196 255 2 61 2.00e-19 81.6
Msa0577500 MtrunA17_Chr3g0085921 60.000 60 24 0 150 209 2 61 5.23e-18 77.8
Msa0577500 MtrunA17_Chr3g0085921 57.627 59 25 0 105 163 3 61 2.41e-17 76.3
Msa0577500 MtrunA17_Chr3g0085921 57.627 59 25 0 59 117 3 61 8.07e-16 72.0
Msa0577500 MtrunA17_Chr1g0210831 37.069 116 73 0 78 193 625 740 3.34e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0577500 AT1G60890.1 49.206 189 96 0 16 204 7 195 1.22e-54 189
Msa0577500 AT1G60890.1 41.209 182 107 0 65 246 10 191 3.33e-40 149
Msa0577500 AT1G60890.1 40.881 159 94 0 111 269 10 168 5.23e-33 128
Msa0577500 AT1G60890.2 49.206 189 96 0 16 204 19 207 1.23e-54 189
Msa0577500 AT1G60890.2 41.209 182 107 0 65 246 22 203 2.98e-40 149
Msa0577500 AT1G60890.2 40.881 159 94 0 111 269 22 180 4.50e-33 128
Msa0577500 AT1G10900.2 46.032 189 102 0 16 204 7 195 7.35e-50 176
Msa0577500 AT1G10900.2 38.798 183 112 0 64 246 9 191 4.66e-37 139
Msa0577500 AT1G10900.2 38.750 160 98 0 110 269 9 168 1.23e-29 118
Msa0577500 AT1G10900.1 46.032 189 102 0 16 204 7 195 7.35e-50 176
Msa0577500 AT1G10900.1 38.798 183 112 0 64 246 9 191 4.66e-37 139
Msa0577500 AT1G10900.1 38.750 160 98 0 110 269 9 168 1.23e-29 118
Msa0577500 AT1G10900.3 46.032 189 102 0 16 204 7 195 7.35e-50 176
Msa0577500 AT1G10900.3 38.798 183 112 0 64 246 9 191 4.66e-37 139
Msa0577500 AT1G10900.3 38.750 160 98 0 110 269 9 168 1.23e-29 118
Msa0577500 AT3G07960.2 40.541 185 110 0 16 200 23 207 3.66e-39 145
Msa0577500 AT3G07960.2 34.634 205 121 2 67 265 28 225 1.40e-31 123
Msa0577500 AT3G07960.2 40.764 157 93 0 112 268 27 183 2.07e-31 123
Msa0577500 AT3G07960.2 38.938 113 69 0 20 132 96 208 1.90e-14 73.9
Msa0577500 AT3G07960.4 40.541 185 110 0 16 200 23 207 3.66e-39 145
Msa0577500 AT3G07960.4 34.634 205 121 2 67 265 28 225 1.40e-31 123
Msa0577500 AT3G07960.4 40.764 157 93 0 112 268 27 183 2.07e-31 123
Msa0577500 AT3G07960.4 38.938 113 69 0 20 132 96 208 1.90e-14 73.9
Msa0577500 AT3G07960.3 40.541 185 110 0 16 200 23 207 5.14e-39 144
Msa0577500 AT3G07960.3 34.634 205 121 2 67 265 28 225 2.09e-31 123
Msa0577500 AT3G07960.3 40.764 157 93 0 112 268 27 183 2.77e-31 122
Msa0577500 AT3G07960.3 38.938 113 69 0 20 132 96 208 2.06e-14 73.6
Msa0577500 AT1G21980.1 41.714 175 102 0 16 190 72 246 5.28e-39 145
Msa0577500 AT1G21980.1 36.216 185 114 1 56 236 62 246 2.21e-30 120
Msa0577500 AT1G21980.1 37.736 159 99 0 112 270 76 234 2.82e-29 117
Msa0577500 AT3G07960.1 40.541 185 110 0 16 200 23 207 1.01e-38 144
Msa0577500 AT3G07960.1 34.634 205 121 2 67 265 28 225 3.04e-31 123
Msa0577500 AT3G07960.1 40.764 157 93 0 112 268 27 183 4.17e-31 122
Msa0577500 AT3G07960.1 38.938 113 69 0 20 132 96 208 2.63e-14 73.6
Msa0577500 AT3G09920.3 41.935 186 108 0 14 199 47 232 1.67e-38 144
Msa0577500 AT3G09920.3 40.437 183 109 0 63 245 50 232 4.05e-33 128
Msa0577500 AT3G09920.3 39.752 161 97 0 109 269 50 210 2.35e-26 108
Msa0577500 AT3G09920.4 41.935 186 108 0 14 199 47 232 1.67e-38 144
Msa0577500 AT3G09920.4 40.437 183 109 0 63 245 50 232 4.05e-33 128
Msa0577500 AT3G09920.4 39.752 161 97 0 109 269 50 210 2.35e-26 108
Msa0577500 AT3G09920.2 41.935 186 108 0 14 199 47 232 1.67e-38 144
Msa0577500 AT3G09920.2 40.437 183 109 0 63 245 50 232 4.05e-33 128
Msa0577500 AT3G09920.2 39.752 161 97 0 109 269 50 210 2.35e-26 108
Msa0577500 AT3G09920.1 41.935 186 108 0 14 199 47 232 1.67e-38 144
Msa0577500 AT3G09920.1 40.437 183 109 0 63 245 50 232 4.05e-33 128
Msa0577500 AT3G09920.1 39.752 161 97 0 109 269 50 210 2.35e-26 108
Msa0577500 AT2G41210.1 36.765 204 120 1 16 210 66 269 8.26e-36 136
Msa0577500 AT2G41210.1 35.754 179 115 0 67 245 71 249 5.16e-28 114
Msa0577500 AT2G41210.1 36.364 165 103 1 104 268 64 226 3.56e-26 108
Msa0577500 AT3G56960.2 36.453 203 120 1 16 209 68 270 9.28e-36 136
Msa0577500 AT3G56960.2 35.196 179 116 0 67 245 73 251 5.76e-29 116
Msa0577500 AT3G56960.2 36.264 182 105 2 87 268 58 228 5.80e-28 113
Msa0577500 AT3G56960.2 33.514 185 123 0 89 273 72 256 1.14e-24 103
Msa0577500 AT3G56960.1 36.453 203 120 1 16 209 68 270 9.28e-36 136
Msa0577500 AT3G56960.1 35.196 179 116 0 67 245 73 251 5.76e-29 116
Msa0577500 AT3G56960.1 36.264 182 105 2 87 268 58 228 5.80e-28 113
Msa0577500 AT3G56960.1 33.514 185 123 0 89 273 72 256 1.14e-24 103
Msa0577500 AT1G21920.2 40.659 182 106 1 14 193 166 347 7.83e-35 129
Msa0577500 AT1G21920.2 36.413 184 117 0 53 236 161 344 2.66e-31 120
Msa0577500 AT1G21920.2 38.650 163 100 0 106 268 168 330 1.70e-28 112
Msa0577500 AT1G21920.2 31.658 199 136 0 84 282 169 367 2.68e-25 103
Msa0577500 AT1G77740.2 39.429 175 106 0 16 190 73 247 9.08e-35 133
Msa0577500 AT1G77740.2 37.647 170 106 0 67 236 78 247 2.80e-28 114
Msa0577500 AT1G77740.2 36.478 159 101 0 112 270 77 235 3.45e-27 111
Msa0577500 AT1G77740.2 33.333 171 114 0 89 259 77 247 5.35e-23 99.4
Msa0577500 AT1G77740.2 36.496 137 87 0 134 270 76 212 1.16e-21 95.1
Msa0577500 AT1G77740.1 39.429 175 106 0 16 190 73 247 9.08e-35 133
Msa0577500 AT1G77740.1 37.647 170 106 0 67 236 78 247 2.80e-28 114
Msa0577500 AT1G77740.1 36.478 159 101 0 112 270 77 235 3.45e-27 111
Msa0577500 AT1G77740.1 33.333 171 114 0 89 259 77 247 5.35e-23 99.4
Msa0577500 AT1G77740.1 36.496 137 87 0 134 270 76 212 1.16e-21 95.1
Msa0577500 AT1G77660.1 40.206 194 114 1 2 193 158 351 1.21e-34 130
Msa0577500 AT1G77660.1 37.222 180 113 0 57 236 169 348 2.14e-29 115
Msa0577500 AT1G77660.1 38.650 163 100 0 106 268 172 334 2.43e-26 107
Msa0577500 AT1G21920.1 40.659 182 106 1 14 193 166 347 1.50e-34 129
Msa0577500 AT1G21920.1 36.413 184 117 0 53 236 161 344 7.94e-31 119
Msa0577500 AT1G21920.1 38.650 163 100 0 106 268 168 330 5.26e-28 112
Msa0577500 AT1G21920.1 31.500 200 137 0 84 283 169 368 8.02e-25 103
Msa0577500 AT2G26420.1 41.463 164 96 0 14 177 47 210 5.96e-32 125
Msa0577500 AT2G26420.1 37.342 158 99 0 113 270 54 211 9.69e-26 107
Msa0577500 AT2G26420.1 36.957 184 114 1 67 248 54 237 1.60e-24 103
Msa0577500 AT2G26420.1 36.875 160 101 0 90 249 54 213 8.82e-23 98.6
Msa0577500 AT2G26420.2 41.463 164 96 0 14 177 47 210 6.98e-32 125
Msa0577500 AT2G26420.2 37.342 158 99 0 113 270 54 211 1.25e-25 106
Msa0577500 AT2G26420.2 36.957 184 114 1 67 248 54 237 2.05e-24 103
Msa0577500 AT2G26420.2 36.875 160 101 0 90 249 54 213 1.14e-22 98.2
Msa0577500 AT2G26420.3 41.463 164 96 0 14 177 47 210 6.98e-32 125
Msa0577500 AT2G26420.3 37.342 158 99 0 113 270 54 211 1.25e-25 106
Msa0577500 AT2G26420.3 36.957 184 114 1 67 248 54 237 2.05e-24 103
Msa0577500 AT2G26420.3 36.875 160 101 0 90 249 54 213 1.14e-22 98.2
Msa0577500 AT4G17080.1 40.000 175 105 0 19 193 256 430 8.91e-32 123
Msa0577500 AT4G17080.1 37.714 175 109 0 42 216 256 430 3.90e-29 116
Msa0577500 AT4G17080.1 38.012 171 106 0 99 269 244 414 1.63e-28 114
Msa0577500 AT4G17080.1 32.487 197 127 1 62 258 253 443 4.07e-28 113
Msa0577500 AT2G35170.1 39.766 171 103 0 23 193 229 399 5.68e-30 118
Msa0577500 AT2G35170.1 32.843 204 134 1 62 262 222 425 2.46e-27 110
Msa0577500 AT2G35170.1 38.824 170 104 0 47 216 230 399 4.54e-27 110
Msa0577500 AT1G75010.1 34.921 126 82 0 68 193 604 729 2.23e-11 64.7

Find 87 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAACGTCTACACTGGAAAT+TGG 0.199973 4_1:+81857565 Msa0577500:CDS
GAAACGTCTACACTGGAAAT+TGG 0.199973 4_1:+81857841 Msa0577500:CDS
TACACTGGAAATTGGGAAAA+AGG 0.217123 4_1:+81857849 Msa0577500:CDS
GGGATGTCTACGCTGGAAAT+TGG 0.240613 4_1:+81857703 Msa0577500:CDS
TACACTGGAAATTGGGAAAA+AGG 0.296129 4_1:+81857573 Msa0577500:CDS
GAGATGTCTACACTGGAAAT+TGG 0.297839 4_1:+81857979 Msa0577500:CDS
TGACCAACAACCATCGAAAA+TGG 0.303459 4_1:-81857499 None:intergenic
TACGCTGGAAATTGGGAAAA+AGG 0.309774 4_1:+81857711 Msa0577500:CDS
AATTAAGCTTCAGATGATAA+TGG 0.335808 4_1:-81858144 None:intergenic
ATGGAAAAGGGATTATGAAT+TGG 0.347632 4_1:+81857742 Msa0577500:CDS
AATGGACTTTCGCATGGATA+TGG 0.353378 4_1:+81857798 Msa0577500:CDS
TACGAGGGCGATTGGGTTGA+TGG 0.368273 4_1:+81858056 Msa0577500:CDS
AATGGACATAAACATGGATA+TGG 0.369212 4_1:+81857660 Msa0577500:CDS
AGGGAAAGGGATTATGAATT+GGG 0.375715 4_1:+81857467 Msa0577500:CDS
GAGTTTATAGATTTGCAAAT+GGG 0.384860 4_1:+81857682 Msa0577500:CDS
CAGGGAAAGGGATTATGAAT+TGG 0.387625 4_1:+81857466 Msa0577500:CDS
TTCAAGGGTGAACTTCTTCA+TGG 0.393069 4_1:+81857378 Msa0577500:CDS
TTCGATGGTTGTTGGTCAAA+TGG 0.393393 4_1:+81857504 Msa0577500:CDS
AATGGACTTTCACATGGATA+TGG 0.393948 4_1:+81857522 Msa0577500:CDS
TGGAAAAGGGATTATGAATT+GGG 0.398832 4_1:+81857743 Msa0577500:CDS
ATTAAGCTTCAGATGATAAT+GGG 0.406796 4_1:-81858143 None:intergenic
TTCGATGGTTATTGGTCAAA+TGG 0.407614 4_1:+81857642 Msa0577500:CDS
TTCGATGGTTATTGGTCAAA+TGG 0.407614 4_1:+81857918 Msa0577500:CDS
TACGAGGGTGACTGGGTTGA+TGG 0.408545 4_1:+81857435 Msa0577500:CDS
AATAATAAAATGGATGGAAG+AGG 0.408648 4_1:+81858005 Msa0577500:CDS
GAACAATATACGAGGGTGAC+TGG 0.412789 4_1:+81857427 Msa0577500:CDS
ATGGAAAAGGGATTATGAAA+TGG 0.419433 4_1:+81857604 Msa0577500:CDS
TTCGATGGTTGCTGGTCAAA+TGG 0.420223 4_1:+81857780 Msa0577500:CDS
GGGATTATGAATTGGGCTAA+TGG 0.426829 4_1:+81857750 Msa0577500:CDS
AAGGGGAAAGGGATGGAAAA+GGG 0.430911 4_1:+81857868 Msa0577500:CDS
TGGGTTGATGGACAAATGAT+AGG 0.434531 4_1:+81858068 Msa0577500:CDS
GGAGTTTATAGATTTGCAAA+TGG 0.437498 4_1:+81857681 Msa0577500:CDS
AAAGGGGAAAGGGATGGAAA+AGG 0.442937 4_1:+81857867 Msa0577500:CDS
ATGGAAAAGGGATTATGAAA+TGG 0.443399 4_1:+81857880 Msa0577500:CDS
AAAGGAGAAAAGGATGGAAA+AGG 0.446263 4_1:+81857729 Msa0577500:CDS
AAACGTCTACACTGGAAATT+GGG 0.451480 4_1:+81857566 Msa0577500:CDS
AAACGTCTACACTGGAAATT+GGG 0.451480 4_1:+81857842 Msa0577500:CDS
AAGGAGAAAAGGATGGAAAA+GGG 0.451607 4_1:+81857730 Msa0577500:CDS
GGGATTATGAATTGGTCAAA+TGG 0.455729 4_1:+81858026 Msa0577500:CDS
GAACAATGTACGAGGGCGAT+TGG 0.457462 4_1:+81858048 Msa0577500:CDS
AAGGTGAAAGGCATGGAAAA+GGG 0.462403 4_1:+81857592 Msa0577500:CDS
GGATGTCTACGCTGGAAATT+GGG 0.465315 4_1:+81857704 Msa0577500:CDS
ATGGAAGAGGGATTATGAAT+TGG 0.469752 4_1:+81858018 Msa0577500:CDS
GGGATTATGAATTGGGGTAA+TGG 0.470324 4_1:+81857474 Msa0577500:CDS
AACAATGTACGAGGGCGATT+GGG 0.470740 4_1:+81858049 Msa0577500:CDS
TGTCTACATTGGTAAATTCA+AGG 0.474127 4_1:+81857362 Msa0577500:CDS
AAAGGTGAAAGGCATGGAAA+AGG 0.474168 4_1:+81857591 Msa0577500:CDS
GGAGTTTATAGATTTGCAAA+TGG 0.480472 4_1:+81857957 Msa0577500:CDS
TGGAAAAGGGATTATGAAAT+GGG 0.487859 4_1:+81857881 Msa0577500:CDS
TGGAAAAGGGATTATGAAAT+GGG 0.493424 4_1:+81857605 Msa0577500:CDS
GGAAAGTACACATGGACAAA+TGG 0.494048 4_1:+81857405 Msa0577500:CDS
GATGGAAAAGTGACAGGGAA+AGG 0.496612 4_1:+81857453 Msa0577500:CDS
GGAGTTTATAGATCTGCAAA+TGG 0.513797 4_1:+81857543 Msa0577500:CDS
GGAGTTTATAGATCTGCAAA+TGG 0.513797 4_1:+81857819 Msa0577500:CDS
TCAAATGGGGATGTCTACAT+TGG 0.518877 4_1:+81857351 Msa0577500:CDS
ATGCAAGTCATGGAGGATGA+TGG 0.528096 4_1:+81857175 Msa0577500:CDS
ATGGAAAAGTGACAGGGAAA+GGG 0.534561 4_1:+81857454 Msa0577500:CDS
TGGGTTGATGGAAAAGTGAC+AGG 0.540161 4_1:+81857447 Msa0577500:CDS
ATAATAAAATGGATGGAAGA+GGG 0.546218 4_1:+81858006 Msa0577500:CDS
GATGGACAAATGATAGGGAA+TGG 0.550634 4_1:+81858074 Msa0577500:CDS
AACAATATACGAGGGTGACT+GGG 0.562591 4_1:+81857428 Msa0577500:CDS
GGGTTGATGGACAAATGATA+GGG 0.565463 4_1:+81858069 Msa0577500:CDS
AGTTTATAGATTTGCAAATG+GGG 0.568661 4_1:+81857683 Msa0577500:CDS
GTCTACATTGGTAAATTCAA+GGG 0.570653 4_1:+81857363 Msa0577500:CDS
AATGGACAGAAACATGGCTA+TGG 0.574469 4_1:+81857936 Msa0577500:CDS
GGTGAACTTCTTCATGGCAT+CGG 0.576407 4_1:+81857384 Msa0577500:CDS
TGGTCAAATGGACTTTCACA+TGG 0.576790 4_1:+81857516 Msa0577500:CDS
GGGAATGGATTGATGATACC+AGG 0.577591 4_1:+81858089 Msa0577500:CDS
GAGTGAAAGAATGCAAGTCA+TGG 0.582412 4_1:+81857165 Msa0577500:CDS
GCAAATGGAAACGTCTACAC+TGG 0.588126 4_1:+81857558 Msa0577500:CDS
GCAAATGGAAACGTCTACAC+TGG 0.588126 4_1:+81857834 Msa0577500:CDS
GCAAATGGGGATGTCTACGC+TGG 0.596613 4_1:+81857696 Msa0577500:CDS
TGGTCAAATGGACAGAAACA+TGG 0.596729 4_1:+81857930 Msa0577500:CDS
TGGTCAAATGGACTTTCGCA+TGG 0.604154 4_1:+81857792 Msa0577500:CDS
TGGTCAAATGGACATAAACA+TGG 0.607218 4_1:+81857654 Msa0577500:CDS
GCAAATGGAGATGTCTACAC+TGG 0.608648 4_1:+81857972 Msa0577500:CDS
GGGATTATGAAATGGGCTAA+CGG 0.622522 4_1:+81857612 Msa0577500:CDS
GGGATTATGAAATGGGCTAA+CGG 0.622522 4_1:+81857888 Msa0577500:CDS
GGGAAAGGGATTATGAATTG+GGG 0.649116 4_1:+81857468 Msa0577500:CDS
AAGTCATGGAGGATGATGGA+AGG 0.670085 4_1:+81857179 Msa0577500:CDS
GTCAAATGGAACAATGTACG+AGG 0.679412 4_1:+81858040 Msa0577500:CDS
ATGGCATCGGAAAGTACACA+TGG 0.681327 4_1:+81857397 Msa0577500:CDS
GACAAATGGAACAATATACG+AGG 0.707016 4_1:+81857419 Msa0577500:CDS
TCAAATGGAACAATGTACGA+GGG 0.718322 4_1:+81858041 Msa0577500:CDS
ACAAATGGAACAATATACGA+GGG 0.718645 4_1:+81857420 Msa0577500:CDS
GGGTTGATGGAAAAGTGACA+GGG 0.720292 4_1:+81857448 Msa0577500:CDS
TGAAAGAATGCAAGTCATGG+AGG 0.727592 4_1:+81857168 Msa0577500:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTGGAAAAATAATAAAA+TGG + chr4_1:81857995-81858014 Msa0577500:CDS 10.0%
!! AAATTGGAAAAATAATAAAA+TGG + chr4_1:81857995-81858014 Msa0577500:CDS 10.0%
!! GAAATCATAATCATAATAAA+TGG - chr4_1:81857291-81857310 None:intergenic 15.0%
!! GAAATCATAATCATAATAAA+TGG - chr4_1:81857291-81857310 None:intergenic 15.0%
!! TGGAAAAATAATAAAATGGA+TGG + chr4_1:81857999-81858018 Msa0577500:CDS 20.0%
!! TGGAAAAATAATAAAATGGA+TGG + chr4_1:81857999-81858018 Msa0577500:CDS 20.0%
!! AAAGAGAAGTTCTTTTCAAA+TGG + chr4_1:81857336-81857355 Msa0577500:CDS 25.0%
!! AAGAGAAGTTCTTTTCAAAT+GGG + chr4_1:81857337-81857356 Msa0577500:CDS 25.0%
! GAGTTTATAGATTTGCAAAT+GGG + chr4_1:81857682-81857701 Msa0577500:CDS 25.0%
! AGTTTATAGATTTGCAAATG+GGG + chr4_1:81857683-81857702 Msa0577500:CDS 25.0%
! AATAATAAAATGGATGGAAG+AGG + chr4_1:81858005-81858024 Msa0577500:CDS 25.0%
! ATAATAAAATGGATGGAAGA+GGG + chr4_1:81858006-81858025 Msa0577500:CDS 25.0%
!! AAAGAGAAGTTCTTTTCAAA+TGG + chr4_1:81857336-81857355 Msa0577500:CDS 25.0%
!! AAGAGAAGTTCTTTTCAAAT+GGG + chr4_1:81857337-81857356 Msa0577500:CDS 25.0%
! GAGTTTATAGATTTGCAAAT+GGG + chr4_1:81857682-81857701 Msa0577500:CDS 25.0%
! AGTTTATAGATTTGCAAATG+GGG + chr4_1:81857683-81857702 Msa0577500:CDS 25.0%
! AATAATAAAATGGATGGAAG+AGG + chr4_1:81858005-81858024 Msa0577500:CDS 25.0%
! ATAATAAAATGGATGGAAGA+GGG + chr4_1:81858006-81858025 Msa0577500:CDS 25.0%
! AGAGAAGTTCTTTTCAAATG+GGG + chr4_1:81857338-81857357 Msa0577500:CDS 30.0%
TGTCTACATTGGTAAATTCA+AGG + chr4_1:81857362-81857381 Msa0577500:CDS 30.0%
GTCTACATTGGTAAATTCAA+GGG + chr4_1:81857363-81857382 Msa0577500:CDS 30.0%
ACAAATGGAACAATATACGA+GGG + chr4_1:81857420-81857439 Msa0577500:CDS 30.0%
ATGGAAAAGGGATTATGAAA+TGG + chr4_1:81857604-81857623 Msa0577500:CDS 30.0%
TGGAAAAGGGATTATGAAAT+GGG + chr4_1:81857605-81857624 Msa0577500:CDS 30.0%
AATGGACATAAACATGGATA+TGG + chr4_1:81857660-81857679 Msa0577500:CDS 30.0%
GGAGTTTATAGATTTGCAAA+TGG + chr4_1:81857681-81857700 Msa0577500:CDS 30.0%
AAATTGGGAAAAAGGAGAAA+AGG + chr4_1:81857719-81857738 Msa0577500:CDS 30.0%
ATGGAAAAGGGATTATGAAT+TGG + chr4_1:81857742-81857761 Msa0577500:CDS 30.0%
TGGAAAAGGGATTATGAATT+GGG + chr4_1:81857743-81857762 Msa0577500:CDS 30.0%
ATGGAAAAGGGATTATGAAA+TGG + chr4_1:81857604-81857623 Msa0577500:CDS 30.0%
TGGAAAAGGGATTATGAAAT+GGG + chr4_1:81857605-81857624 Msa0577500:CDS 30.0%
GGAGTTTATAGATTTGCAAA+TGG + chr4_1:81857681-81857700 Msa0577500:CDS 30.0%
AAAAATGTACATAGAAAGCC+TGG - chr4_1:81858110-81858129 None:intergenic 30.0%
! AGAGAAGTTCTTTTCAAATG+GGG + chr4_1:81857338-81857357 Msa0577500:CDS 30.0%
TGTCTACATTGGTAAATTCA+AGG + chr4_1:81857362-81857381 Msa0577500:CDS 30.0%
GTCTACATTGGTAAATTCAA+GGG + chr4_1:81857363-81857382 Msa0577500:CDS 30.0%
ACAAATGGAACAATATACGA+GGG + chr4_1:81857420-81857439 Msa0577500:CDS 30.0%
ATGGAAAAGGGATTATGAAA+TGG + chr4_1:81857604-81857623 Msa0577500:CDS 30.0%
TGGAAAAGGGATTATGAAAT+GGG + chr4_1:81857605-81857624 Msa0577500:CDS 30.0%
AATGGACATAAACATGGATA+TGG + chr4_1:81857660-81857679 Msa0577500:CDS 30.0%
GGAGTTTATAGATTTGCAAA+TGG + chr4_1:81857681-81857700 Msa0577500:CDS 30.0%
AAATTGGGAAAAAGGAGAAA+AGG + chr4_1:81857719-81857738 Msa0577500:CDS 30.0%
ATGGAAAAGGGATTATGAAT+TGG + chr4_1:81857742-81857761 Msa0577500:CDS 30.0%
TGGAAAAGGGATTATGAATT+GGG + chr4_1:81857743-81857762 Msa0577500:CDS 30.0%
ATGGAAAAGGGATTATGAAA+TGG + chr4_1:81857604-81857623 Msa0577500:CDS 30.0%
TGGAAAAGGGATTATGAAAT+GGG + chr4_1:81857605-81857624 Msa0577500:CDS 30.0%
GGAGTTTATAGATTTGCAAA+TGG + chr4_1:81857681-81857700 Msa0577500:CDS 30.0%
AAAAATGTACATAGAAAGCC+TGG - chr4_1:81858110-81858129 None:intergenic 30.0%
TGAATTAGATAGAGTAGCTG+TGG - chr4_1:81857220-81857239 None:intergenic 35.0%
GACAAATGGAACAATATACG+AGG + chr4_1:81857419-81857438 Msa0577500:CDS 35.0%
AGGGAAAGGGATTATGAATT+GGG + chr4_1:81857467-81857486 Msa0577500:CDS 35.0%
! AATGGACTTTCACATGGATA+TGG + chr4_1:81857522-81857541 Msa0577500:CDS 35.0%
GGAGTTTATAGATCTGCAAA+TGG + chr4_1:81857543-81857562 Msa0577500:CDS 35.0%
AAACGTCTACACTGGAAATT+GGG + chr4_1:81857566-81857585 Msa0577500:CDS 35.0%
TACACTGGAAATTGGGAAAA+AGG + chr4_1:81857573-81857592 Msa0577500:CDS 35.0%
GAAATTGGGAAAAAGGTGAA+AGG + chr4_1:81857580-81857599 Msa0577500:CDS 35.0%
! GTGATGTTTTCGATGGTTAT+TGG + chr4_1:81857634-81857653 Msa0577500:CDS 35.0%
TTCGATGGTTATTGGTCAAA+TGG + chr4_1:81857642-81857661 Msa0577500:CDS 35.0%
TGGTCAAATGGACATAAACA+TGG + chr4_1:81857654-81857673 Msa0577500:CDS 35.0%
AAAGGAGAAAAGGATGGAAA+AGG + chr4_1:81857729-81857748 Msa0577500:CDS 35.0%
AAGGAGAAAAGGATGGAAAA+GGG + chr4_1:81857730-81857749 Msa0577500:CDS 35.0%
GGAGTTTATAGATCTGCAAA+TGG + chr4_1:81857543-81857562 Msa0577500:CDS 35.0%
AAACGTCTACACTGGAAATT+GGG + chr4_1:81857566-81857585 Msa0577500:CDS 35.0%
TACACTGGAAATTGGGAAAA+AGG + chr4_1:81857573-81857592 Msa0577500:CDS 35.0%
ACACTGGAAATTGGGAAAAA+GGG + chr4_1:81857574-81857593 Msa0577500:CDS 35.0%
AAATTGGGAAAAAGGGGAAA+GGG + chr4_1:81857857-81857876 Msa0577500:CDS 35.0%
! GTGATGTTTTCGATGGTTAT+TGG + chr4_1:81857634-81857653 Msa0577500:CDS 35.0%
TTCGATGGTTATTGGTCAAA+TGG + chr4_1:81857642-81857661 Msa0577500:CDS 35.0%
ATGGAAGAGGGATTATGAAT+TGG + chr4_1:81858018-81858037 Msa0577500:CDS 35.0%
GGGATTATGAATTGGTCAAA+TGG + chr4_1:81858026-81858045 Msa0577500:CDS 35.0%
TCAAATGGAACAATGTACGA+GGG + chr4_1:81858041-81858060 Msa0577500:CDS 35.0%
TGAATTAGATAGAGTAGCTG+TGG - chr4_1:81857220-81857239 None:intergenic 35.0%
GACAAATGGAACAATATACG+AGG + chr4_1:81857419-81857438 Msa0577500:CDS 35.0%
AGGGAAAGGGATTATGAATT+GGG + chr4_1:81857467-81857486 Msa0577500:CDS 35.0%
! AATGGACTTTCACATGGATA+TGG + chr4_1:81857522-81857541 Msa0577500:CDS 35.0%
GGAGTTTATAGATCTGCAAA+TGG + chr4_1:81857543-81857562 Msa0577500:CDS 35.0%
AAACGTCTACACTGGAAATT+GGG + chr4_1:81857566-81857585 Msa0577500:CDS 35.0%
TACACTGGAAATTGGGAAAA+AGG + chr4_1:81857573-81857592 Msa0577500:CDS 35.0%
GAAATTGGGAAAAAGGTGAA+AGG + chr4_1:81857580-81857599 Msa0577500:CDS 35.0%
! GTGATGTTTTCGATGGTTAT+TGG + chr4_1:81857634-81857653 Msa0577500:CDS 35.0%
TTCGATGGTTATTGGTCAAA+TGG + chr4_1:81857642-81857661 Msa0577500:CDS 35.0%
TGGTCAAATGGACATAAACA+TGG + chr4_1:81857654-81857673 Msa0577500:CDS 35.0%
AAAGGAGAAAAGGATGGAAA+AGG + chr4_1:81857729-81857748 Msa0577500:CDS 35.0%
AAGGAGAAAAGGATGGAAAA+GGG + chr4_1:81857730-81857749 Msa0577500:CDS 35.0%
GGAGTTTATAGATCTGCAAA+TGG + chr4_1:81857543-81857562 Msa0577500:CDS 35.0%
AAACGTCTACACTGGAAATT+GGG + chr4_1:81857566-81857585 Msa0577500:CDS 35.0%
TACACTGGAAATTGGGAAAA+AGG + chr4_1:81857573-81857592 Msa0577500:CDS 35.0%
ACACTGGAAATTGGGAAAAA+GGG + chr4_1:81857574-81857593 Msa0577500:CDS 35.0%
AAATTGGGAAAAAGGGGAAA+GGG + chr4_1:81857857-81857876 Msa0577500:CDS 35.0%
! GTGATGTTTTCGATGGTTAT+TGG + chr4_1:81857634-81857653 Msa0577500:CDS 35.0%
TTCGATGGTTATTGGTCAAA+TGG + chr4_1:81857642-81857661 Msa0577500:CDS 35.0%
ATGGAAGAGGGATTATGAAT+TGG + chr4_1:81858018-81858037 Msa0577500:CDS 35.0%
GGGATTATGAATTGGTCAAA+TGG + chr4_1:81858026-81858045 Msa0577500:CDS 35.0%
TCAAATGGAACAATGTACGA+GGG + chr4_1:81858041-81858060 Msa0577500:CDS 35.0%
GAGTGAAAGAATGCAAGTCA+TGG + chr4_1:81857165-81857184 Msa0577500:CDS 40.0%
TGAAAGAATGCAAGTCATGG+AGG + chr4_1:81857168-81857187 Msa0577500:CDS 40.0%
TCAAATGGGGATGTCTACAT+TGG + chr4_1:81857351-81857370 Msa0577500:CDS 40.0%
TTCAAGGGTGAACTTCTTCA+TGG + chr4_1:81857378-81857397 Msa0577500:CDS 40.0%
! GGAAAGTACACATGGACAAA+TGG + chr4_1:81857405-81857424 Msa0577500:CDS 40.0%
AACAATATACGAGGGTGACT+GGG + chr4_1:81857428-81857447 Msa0577500:CDS 40.0%
ATGGAAAAGTGACAGGGAAA+GGG + chr4_1:81857454-81857473 Msa0577500:CDS 40.0%
CAGGGAAAGGGATTATGAAT+TGG + chr4_1:81857466-81857485 Msa0577500:CDS 40.0%
GGGAAAGGGATTATGAATTG+GGG + chr4_1:81857468-81857487 Msa0577500:CDS 40.0%
GGGATTATGAATTGGGGTAA+TGG + chr4_1:81857474-81857493 Msa0577500:CDS 40.0%
TGACCAACAACCATCGAAAA+TGG - chr4_1:81857502-81857521 None:intergenic 40.0%
TTCGATGGTTGTTGGTCAAA+TGG + chr4_1:81857504-81857523 Msa0577500:CDS 40.0%
! TGGTCAAATGGACTTTCACA+TGG + chr4_1:81857516-81857535 Msa0577500:CDS 40.0%
GAAACGTCTACACTGGAAAT+TGG + chr4_1:81857565-81857584 Msa0577500:CDS 40.0%
! AAAGGTGAAAGGCATGGAAA+AGG + chr4_1:81857591-81857610 Msa0577500:CDS 40.0%
! AAGGTGAAAGGCATGGAAAA+GGG + chr4_1:81857592-81857611 Msa0577500:CDS 40.0%
!! GGGATTATGAAATGGGCTAA+CGG + chr4_1:81857612-81857631 Msa0577500:CDS 40.0%
TACGCTGGAAATTGGGAAAA+AGG + chr4_1:81857711-81857730 Msa0577500:CDS 40.0%
TGGGAAAAAGGAGAAAAGGA+TGG + chr4_1:81857723-81857742 Msa0577500:CDS 40.0%
GGGATTATGAATTGGGCTAA+TGG + chr4_1:81857750-81857769 Msa0577500:CDS 40.0%
!!! GCTAATGGTGCTATTTTCGA+TGG + chr4_1:81857765-81857784 Msa0577500:CDS 40.0%
! AATGGACTTTCGCATGGATA+TGG + chr4_1:81857798-81857817 Msa0577500:CDS 40.0%
GAAACGTCTACACTGGAAAT+TGG + chr4_1:81857565-81857584 Msa0577500:CDS 40.0%
CACTGGAAATTGGGAAAAAG+GGG + chr4_1:81857575-81857594 Msa0577500:CDS 40.0%
GAAATTGGGAAAAAGGGGAA+AGG + chr4_1:81857856-81857875 Msa0577500:CDS 40.0%
!! GGGATTATGAAATGGGCTAA+CGG + chr4_1:81857612-81857631 Msa0577500:CDS 40.0%
TGGTCAAATGGACAGAAACA+TGG + chr4_1:81857930-81857949 Msa0577500:CDS 40.0%
AATGGACAGAAACATGGCTA+TGG + chr4_1:81857936-81857955 Msa0577500:CDS 40.0%
GAGATGTCTACACTGGAAAT+TGG + chr4_1:81857979-81857998 Msa0577500:CDS 40.0%
GTCAAATGGAACAATGTACG+AGG + chr4_1:81858040-81858059 Msa0577500:CDS 40.0%
!! TGGGTTGATGGACAAATGAT+AGG + chr4_1:81858068-81858087 Msa0577500:CDS 40.0%
!! GGGTTGATGGACAAATGATA+GGG + chr4_1:81858069-81858088 Msa0577500:CDS 40.0%
GATGGACAAATGATAGGGAA+TGG + chr4_1:81858074-81858093 Msa0577500:CDS 40.0%
GAGTGAAAGAATGCAAGTCA+TGG + chr4_1:81857165-81857184 Msa0577500:CDS 40.0%
TGAAAGAATGCAAGTCATGG+AGG + chr4_1:81857168-81857187 Msa0577500:CDS 40.0%
TCAAATGGGGATGTCTACAT+TGG + chr4_1:81857351-81857370 Msa0577500:CDS 40.0%
TTCAAGGGTGAACTTCTTCA+TGG + chr4_1:81857378-81857397 Msa0577500:CDS 40.0%
! GGAAAGTACACATGGACAAA+TGG + chr4_1:81857405-81857424 Msa0577500:CDS 40.0%
AACAATATACGAGGGTGACT+GGG + chr4_1:81857428-81857447 Msa0577500:CDS 40.0%
ATGGAAAAGTGACAGGGAAA+GGG + chr4_1:81857454-81857473 Msa0577500:CDS 40.0%
CAGGGAAAGGGATTATGAAT+TGG + chr4_1:81857466-81857485 Msa0577500:CDS 40.0%
GGGAAAGGGATTATGAATTG+GGG + chr4_1:81857468-81857487 Msa0577500:CDS 40.0%
GGGATTATGAATTGGGGTAA+TGG + chr4_1:81857474-81857493 Msa0577500:CDS 40.0%
TGACCAACAACCATCGAAAA+TGG - chr4_1:81857502-81857521 None:intergenic 40.0%
TTCGATGGTTGTTGGTCAAA+TGG + chr4_1:81857504-81857523 Msa0577500:CDS 40.0%
! TGGTCAAATGGACTTTCACA+TGG + chr4_1:81857516-81857535 Msa0577500:CDS 40.0%
GAAACGTCTACACTGGAAAT+TGG + chr4_1:81857565-81857584 Msa0577500:CDS 40.0%
! AAAGGTGAAAGGCATGGAAA+AGG + chr4_1:81857591-81857610 Msa0577500:CDS 40.0%
! AAGGTGAAAGGCATGGAAAA+GGG + chr4_1:81857592-81857611 Msa0577500:CDS 40.0%
!! GGGATTATGAAATGGGCTAA+CGG + chr4_1:81857612-81857631 Msa0577500:CDS 40.0%
TACGCTGGAAATTGGGAAAA+AGG + chr4_1:81857711-81857730 Msa0577500:CDS 40.0%
TGGGAAAAAGGAGAAAAGGA+TGG + chr4_1:81857723-81857742 Msa0577500:CDS 40.0%
GGGATTATGAATTGGGCTAA+TGG + chr4_1:81857750-81857769 Msa0577500:CDS 40.0%
!!! GCTAATGGTGCTATTTTCGA+TGG + chr4_1:81857765-81857784 Msa0577500:CDS 40.0%
! AATGGACTTTCGCATGGATA+TGG + chr4_1:81857798-81857817 Msa0577500:CDS 40.0%
GAAACGTCTACACTGGAAAT+TGG + chr4_1:81857565-81857584 Msa0577500:CDS 40.0%
CACTGGAAATTGGGAAAAAG+GGG + chr4_1:81857575-81857594 Msa0577500:CDS 40.0%
GAAATTGGGAAAAAGGGGAA+AGG + chr4_1:81857856-81857875 Msa0577500:CDS 40.0%
!! GGGATTATGAAATGGGCTAA+CGG + chr4_1:81857612-81857631 Msa0577500:CDS 40.0%
TGGTCAAATGGACAGAAACA+TGG + chr4_1:81857930-81857949 Msa0577500:CDS 40.0%
AATGGACAGAAACATGGCTA+TGG + chr4_1:81857936-81857955 Msa0577500:CDS 40.0%
GAGATGTCTACACTGGAAAT+TGG + chr4_1:81857979-81857998 Msa0577500:CDS 40.0%
GTCAAATGGAACAATGTACG+AGG + chr4_1:81858040-81858059 Msa0577500:CDS 40.0%
!! TGGGTTGATGGACAAATGAT+AGG + chr4_1:81858068-81858087 Msa0577500:CDS 40.0%
!! GGGTTGATGGACAAATGATA+GGG + chr4_1:81858069-81858088 Msa0577500:CDS 40.0%
GATGGACAAATGATAGGGAA+TGG + chr4_1:81858074-81858093 Msa0577500:CDS 40.0%
ATGCAAGTCATGGAGGATGA+TGG + chr4_1:81857175-81857194 Msa0577500:CDS 45.0%
!! AAGTCATGGAGGATGATGGA+AGG + chr4_1:81857179-81857198 Msa0577500:CDS 45.0%
GGTGAACTTCTTCATGGCAT+CGG + chr4_1:81857384-81857403 Msa0577500:CDS 45.0%
! ATGGCATCGGAAAGTACACA+TGG + chr4_1:81857397-81857416 Msa0577500:CDS 45.0%
GAACAATATACGAGGGTGAC+TGG + chr4_1:81857427-81857446 Msa0577500:CDS 45.0%
!! TGGGTTGATGGAAAAGTGAC+AGG + chr4_1:81857447-81857466 Msa0577500:CDS 45.0%
!! GGGTTGATGGAAAAGTGACA+GGG + chr4_1:81857448-81857467 Msa0577500:CDS 45.0%
GATGGAAAAGTGACAGGGAA+AGG + chr4_1:81857453-81857472 Msa0577500:CDS 45.0%
!!! GGTAATGGTGCCATTTTCGA+TGG + chr4_1:81857489-81857508 Msa0577500:CDS 45.0%
!!! GTGCCATTTTCGATGGTTGT+TGG + chr4_1:81857496-81857515 Msa0577500:CDS 45.0%
GCAAATGGAAACGTCTACAC+TGG + chr4_1:81857558-81857577 Msa0577500:CDS 45.0%
TGGGAAAAAGGTGAAAGGCA+TGG + chr4_1:81857585-81857604 Msa0577500:CDS 45.0%
! GCTAACGGTGATGTTTTCGA+TGG + chr4_1:81857627-81857646 Msa0577500:CDS 45.0%
GGATGTCTACGCTGGAAATT+GGG + chr4_1:81857704-81857723 Msa0577500:CDS 45.0%
!!! GTGCTATTTTCGATGGTTGC+TGG + chr4_1:81857772-81857791 Msa0577500:CDS 45.0%
TTCGATGGTTGCTGGTCAAA+TGG + chr4_1:81857780-81857799 Msa0577500:CDS 45.0%
! TGGTCAAATGGACTTTCGCA+TGG + chr4_1:81857792-81857811 Msa0577500:CDS 45.0%
GCAAATGGAAACGTCTACAC+TGG + chr4_1:81857558-81857577 Msa0577500:CDS 45.0%
AAAGGGGAAAGGGATGGAAA+AGG + chr4_1:81857867-81857886 Msa0577500:CDS 45.0%
AAGGGGAAAGGGATGGAAAA+GGG + chr4_1:81857868-81857887 Msa0577500:CDS 45.0%
! GCTAACGGTGATGTTTTCGA+TGG + chr4_1:81857627-81857646 Msa0577500:CDS 45.0%
GCAAATGGAGATGTCTACAC+TGG + chr4_1:81857972-81857991 Msa0577500:CDS 45.0%
AACAATGTACGAGGGCGATT+GGG + chr4_1:81858049-81858068 Msa0577500:CDS 45.0%
!! GGGAATGGATTGATGATACC+AGG + chr4_1:81858089-81858108 Msa0577500:CDS 45.0%
ATGCAAGTCATGGAGGATGA+TGG + chr4_1:81857175-81857194 Msa0577500:CDS 45.0%
!! AAGTCATGGAGGATGATGGA+AGG + chr4_1:81857179-81857198 Msa0577500:CDS 45.0%
GGTGAACTTCTTCATGGCAT+CGG + chr4_1:81857384-81857403 Msa0577500:CDS 45.0%
! ATGGCATCGGAAAGTACACA+TGG + chr4_1:81857397-81857416 Msa0577500:CDS 45.0%
GAACAATATACGAGGGTGAC+TGG + chr4_1:81857427-81857446 Msa0577500:CDS 45.0%
!! TGGGTTGATGGAAAAGTGAC+AGG + chr4_1:81857447-81857466 Msa0577500:CDS 45.0%
!! GGGTTGATGGAAAAGTGACA+GGG + chr4_1:81857448-81857467 Msa0577500:CDS 45.0%
GATGGAAAAGTGACAGGGAA+AGG + chr4_1:81857453-81857472 Msa0577500:CDS 45.0%
!!! GGTAATGGTGCCATTTTCGA+TGG + chr4_1:81857489-81857508 Msa0577500:CDS 45.0%
!!! GTGCCATTTTCGATGGTTGT+TGG + chr4_1:81857496-81857515 Msa0577500:CDS 45.0%
GCAAATGGAAACGTCTACAC+TGG + chr4_1:81857558-81857577 Msa0577500:CDS 45.0%
TGGGAAAAAGGTGAAAGGCA+TGG + chr4_1:81857585-81857604 Msa0577500:CDS 45.0%
! GCTAACGGTGATGTTTTCGA+TGG + chr4_1:81857627-81857646 Msa0577500:CDS 45.0%
GGATGTCTACGCTGGAAATT+GGG + chr4_1:81857704-81857723 Msa0577500:CDS 45.0%
!!! GTGCTATTTTCGATGGTTGC+TGG + chr4_1:81857772-81857791 Msa0577500:CDS 45.0%
TTCGATGGTTGCTGGTCAAA+TGG + chr4_1:81857780-81857799 Msa0577500:CDS 45.0%
! TGGTCAAATGGACTTTCGCA+TGG + chr4_1:81857792-81857811 Msa0577500:CDS 45.0%
GCAAATGGAAACGTCTACAC+TGG + chr4_1:81857558-81857577 Msa0577500:CDS 45.0%
AAAGGGGAAAGGGATGGAAA+AGG + chr4_1:81857867-81857886 Msa0577500:CDS 45.0%
AAGGGGAAAGGGATGGAAAA+GGG + chr4_1:81857868-81857887 Msa0577500:CDS 45.0%
! GCTAACGGTGATGTTTTCGA+TGG + chr4_1:81857627-81857646 Msa0577500:CDS 45.0%
GCAAATGGAGATGTCTACAC+TGG + chr4_1:81857972-81857991 Msa0577500:CDS 45.0%
AACAATGTACGAGGGCGATT+GGG + chr4_1:81858049-81858068 Msa0577500:CDS 45.0%
!! GGGAATGGATTGATGATACC+AGG + chr4_1:81858089-81858108 Msa0577500:CDS 45.0%
GGGATGTCTACGCTGGAAAT+TGG + chr4_1:81857703-81857722 Msa0577500:CDS 50.0%
TGGGAAAAAGGGGAAAGGGA+TGG + chr4_1:81857861-81857880 Msa0577500:CDS 50.0%
GAACAATGTACGAGGGCGAT+TGG + chr4_1:81858048-81858067 Msa0577500:CDS 50.0%
GGGATGTCTACGCTGGAAAT+TGG + chr4_1:81857703-81857722 Msa0577500:CDS 50.0%
TGGGAAAAAGGGGAAAGGGA+TGG + chr4_1:81857861-81857880 Msa0577500:CDS 50.0%
GAACAATGTACGAGGGCGAT+TGG + chr4_1:81858048-81858067 Msa0577500:CDS 50.0%
! TACGAGGGTGACTGGGTTGA+TGG + chr4_1:81857435-81857454 Msa0577500:CDS 55.0%
GCAAATGGGGATGTCTACGC+TGG + chr4_1:81857696-81857715 Msa0577500:CDS 55.0%
!! TACGAGGGCGATTGGGTTGA+TGG + chr4_1:81858056-81858075 Msa0577500:CDS 55.0%
! TACGAGGGTGACTGGGTTGA+TGG + chr4_1:81857435-81857454 Msa0577500:CDS 55.0%
GCAAATGGGGATGTCTACGC+TGG + chr4_1:81857696-81857715 Msa0577500:CDS 55.0%
!! TACGAGGGCGATTGGGTTGA+TGG + chr4_1:81858056-81858075 Msa0577500:CDS 55.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 81857163 81858157 81857163 ID=Msa0577500;Name=Msa0577500
chr4_1 mRNA 81857163 81858157 81857163 ID=Msa0577500-mRNA-1;Parent=Msa0577500;Name=Msa0577500-mRNA-1;_AED=0.47;_eAED=0.47;_QI=0|0|0|1|1|1|2|0|287
chr4_1 exon 81857163 81857200 81857163 ID=Msa0577500-mRNA-1:exon:29525;Parent=Msa0577500-mRNA-1
chr4_1 exon 81857332 81858157 81857332 ID=Msa0577500-mRNA-1:exon:29526;Parent=Msa0577500-mRNA-1
chr4_1 CDS 81857163 81857200 81857163 ID=Msa0577500-mRNA-1:cds;Parent=Msa0577500-mRNA-1
chr4_1 CDS 81857332 81858157 81857332 ID=Msa0577500-mRNA-1:cds;Parent=Msa0577500-mRNA-1
Gene Sequence

>Msa0577500

ATGAGTGAAAGAATGCAAGTCATGGAGGATGATGGAAGTGTTAAAGAGAAGTTCTTTTCAAATGGGGATGTCTACATTGGTAAATTCAAGGGTGAACTTCTTCATGGCATCGGAAAGTACACATGGACAAATGGAACAATATACGAGGGTGACTGGGTTGATGGAAAAGTGACAGGGAAAGGGATTATGAATTGGGGTAATGGTGCCATTTTCGATGGTTGTTGGTCAAATGGACTTTCACATGGATATGGAGTTTATAGATCTGCAAATGGAAACGTCTACACTGGAAATTGGGAAAAAGGTGAAAGGCATGGAAAAGGGATTATGAAATGGGCTAACGGTGATGTTTTCGATGGTTATTGGTCAAATGGACATAAACATGGATATGGAGTTTATAGATTTGCAAATGGGGATGTCTACGCTGGAAATTGGGAAAAAGGAGAAAAGGATGGAAAAGGGATTATGAATTGGGCTAATGGTGCTATTTTCGATGGTTGCTGGTCAAATGGACTTTCGCATGGATATGGAGTTTATAGATCTGCAAATGGAAACGTCTACACTGGAAATTGGGAAAAAGGGGAAAGGGATGGAAAAGGGATTATGAAATGGGCTAACGGTGATGTTTTCGATGGTTATTGGTCAAATGGACAGAAACATGGCTATGGAGTTTATAGATTTGCAAATGGAGATGTCTACACTGGAAATTGGAAAAATAATAAAATGGATGGAAGAGGGATTATGAATTGGTCAAATGGAACAATGTACGAGGGCGATTGGGTTGATGGACAAATGATAGGGAATGGATTGATGATACCAGGCTTTCTATGTACATTTTTGAAGACTATCAGACCCATTATCATCTGA
Protein sequence

>Msa0577500

MSERMQVMEDDGSVKEKFFSNGDVYIGKFKGELLHGIGKYTWTNGTIYEGDWVDGKVTGKGIMNWGNGAIFDGCWSNGLSHGYGVYRSANGNVYTGNWEKGERHGKGIMKWANGDVFDGYWSNGHKHGYGVYRFANGDVYAGNWEKGEKDGKGIMNWANGAIFDGCWSNGLSHGYGVYRSANGNVYTGNWEKGERDGKGIMKWANGDVFDGYWSNGQKHGYGVYRFANGDVYTGNWKNNKMDGRGIMNWSNGTMYEGDWVDGQMIGNGLMIPGFLCTFLKTIRPIII*