AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0062440


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0062440 MtrunA17_Chr1g0162931 100.000 68 0 0 1 68 1 68 8.16e-45 144
Msa0062440 MtrunA17_Chr1g0210911 87.143 70 6 1 2 68 3 72 9.99e-39 130
Msa0062440 MtrunA17_Chr2g0288601 74.603 63 16 0 6 68 59 121 6.23e-31 104
Msa0062440 MtrunA17_Chr2g0320231 73.438 64 17 0 5 68 84 147 1.95e-28 103
Msa0062440 MtrunA17_Chr8g0379121 71.429 63 18 0 6 68 99 161 2.96e-28 103
Msa0062440 MtrunA17_Chr7g0243081 65.152 66 23 0 3 68 56 121 5.42e-28 102
Msa0062440 MtrunA17_Chr2g0283951 73.016 63 17 0 6 68 77 139 6.28e-27 99.8
Msa0062440 MtrunA17_Chr3g0095611 64.407 59 21 0 10 68 11 69 9.45e-23 85.9
Msa0062440 MtrunA17_Chr6g0479431 67.308 52 17 0 17 68 201 252 4.67e-21 84.0
Msa0062440 MtrunA17_Chr8g0366281 69.231 52 16 0 17 68 172 223 6.33e-21 83.6
Msa0062440 MtrunA17_Chr5g0410741 67.308 52 17 0 17 68 218 269 4.06e-20 81.6
Msa0062440 MtrunA17_Chr3g0109981 67.308 52 17 0 17 68 273 324 7.27e-20 80.9
Msa0062440 MtrunA17_Chr1g0190301 72.000 50 14 0 19 68 176 225 1.15e-19 80.1
Msa0062440 MtrunA17_Chr7g0274471 70.000 50 15 0 19 68 219 268 1.95e-19 79.7
Msa0062440 MtrunA17_Chr7g0261911 70.000 50 15 0 19 68 263 312 3.75e-19 79.0
Msa0062440 MtrunA17_Chr1g0154261 61.017 59 21 1 10 68 120 176 4.06e-19 77.8
Msa0062440 MtrunA17_Chr5g0409731 56.250 64 28 0 5 68 12 75 3.24e-18 75.5
Msa0062440 MtrunA17_Chr6g0461641 62.000 50 19 0 19 68 43 92 6.01e-17 72.8
Msa0062440 MtrunA17_Chr5g0417521 60.000 50 20 0 19 68 16 65 1.01e-16 72.0
Msa0062440 MtrunA17_Chr8g0356501 64.000 50 18 0 19 68 38 87 1.26e-16 71.6
Msa0062440 MtrunA17_Chr7g0258551 58.182 55 23 0 14 68 208 262 5.85e-16 70.1
Msa0062440 MtrunA17_Chr2g0321461 60.000 50 20 0 19 68 46 95 6.29e-16 69.7
Msa0062440 MtrunA17_Chr1g0175091 60.377 53 21 0 16 68 256 308 6.32e-16 69.7
Msa0062440 MtrunA17_Chr2g0310011 64.000 50 18 0 19 68 36 85 7.17e-16 69.7
Msa0062440 MtrunA17_Chr1g0197171 62.000 50 19 0 19 68 45 94 9.71e-16 68.9
Msa0062440 MtrunA17_Chr2g0290991 62.000 50 19 0 19 68 40 89 1.13e-15 68.9
Msa0062440 MtrunA17_Chr2g0291021 67.347 49 16 0 20 68 51 99 4.38e-15 67.4
Msa0062440 MtrunA17_Chr7g0254981 67.347 49 16 0 20 68 52 100 4.93e-15 66.6
Msa0062440 MtrunA17_Chr1g0175081 44.118 68 38 0 1 68 5 72 9.74e-15 62.8
Msa0062440 MtrunA17_Chr4g0042451 58.000 50 21 0 19 68 18 67 9.90e-15 66.2
Msa0062440 MtrunA17_Chr4g0009901 47.692 65 32 2 5 68 154 217 7.12e-14 63.5
Msa0062440 MtrunA17_Chr4g0045361 63.043 46 17 0 23 68 279 324 7.30e-14 63.9
Msa0062440 MtrunA17_Chr1g0175071 59.184 49 20 0 20 68 179 227 1.75e-13 62.8
Msa0062440 MtrunA17_Chr4g0062921 60.870 46 18 0 23 68 240 285 1.97e-12 60.1
Msa0062440 MtrunA17_Chr7g0258531 53.061 49 23 0 20 68 215 263 3.14e-12 59.3
Msa0062440 MtrunA17_Chr2g0304841 50.000 52 25 1 17 68 212 262 7.07e-12 58.5
Msa0062440 MtrunA17_Chr4g0009991 54.167 48 21 1 21 68 70 116 9.90e-12 55.5
Msa0062440 MtrunA17_Chr4g0009981 50.943 53 25 1 16 68 114 165 1.68e-11 57.0
Msa0062440 MtrunA17_Chr7g0255711 55.769 52 22 1 17 68 90 140 2.13e-11 57.0
Msa0062440 MtrunA17_Chr4g0074421 55.769 52 22 1 17 68 132 182 3.19e-11 55.5
Msa0062440 MtrunA17_Chr5g0422621 54.348 46 21 0 23 68 203 248 3.24e-11 56.6
Msa0062440 MtrunA17_Chr4g0031961 48.387 62 31 1 7 68 120 180 4.37e-11 56.2
Msa0062440 MtrunA17_Chr1g0161431 52.830 53 24 1 16 68 198 249 4.98e-11 56.2
Msa0062440 MtrunA17_Chr7g0242521 55.769 52 20 1 20 68 15 66 5.54e-11 55.5
Msa0062440 MtrunA17_Chr4g0067981 53.846 52 23 1 17 68 214 264 6.32e-11 55.8
Msa0062440 MtrunA17_Chr5g0402631 52.830 53 25 0 16 68 212 264 7.38e-11 55.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0062440 AT1G14600.1 72.603 73 15 1 1 68 1 73 2.60e-31 109
Msa0062440 AT2G02060.2 81.356 59 11 0 10 68 21 79 3.65e-30 106
Msa0062440 AT2G02060.1 81.356 59 11 0 10 68 21 79 3.65e-30 106
Msa0062440 AT4G04605.1 75.000 60 15 0 9 68 5 64 2.11e-28 101
Msa0062440 AT2G40260.1 75.410 61 15 0 8 68 72 132 1.38e-26 99.8
Msa0062440 AT2G38300.1 72.881 59 16 0 10 68 46 104 5.78e-25 94.4
Msa0062440 AT2G42660.1 67.797 59 19 0 10 68 42 100 6.87e-25 92.8
Msa0062440 AT4G04555.1 58.730 63 26 0 6 68 2 64 7.70e-22 81.3
Msa0062440 AT1G32240.2 67.308 52 17 0 17 68 212 263 3.54e-20 82.0
Msa0062440 AT1G32240.1 67.308 52 17 0 17 68 212 263 5.59e-20 81.6
Msa0062440 AT4G04580.1 58.333 60 25 0 9 68 5 64 1.46e-19 77.0
Msa0062440 AT5G16560.1 67.308 52 17 0 17 68 218 269 3.95e-19 79.3
Msa0062440 AT5G42630.2 63.462 52 19 0 17 68 104 155 4.72e-19 77.0
Msa0062440 AT4G17695.1 63.462 52 19 0 17 68 163 214 5.21e-19 78.2
Msa0062440 AT5G42630.1 63.462 52 19 0 17 68 104 155 1.42e-18 76.6
Msa0062440 AT4G28610.1 67.347 49 16 0 20 68 227 275 8.58e-18 75.5
Msa0062440 AT3G04450.3 65.306 49 17 0 20 68 240 288 2.87e-17 73.6
Msa0062440 AT5G29000.4 65.306 49 17 0 20 68 233 281 3.76e-17 73.2
Msa0062440 AT5G45580.2 62.963 54 20 0 15 68 20 73 8.52e-17 72.0
Msa0062440 AT5G45580.1 62.963 54 20 0 15 68 4 57 9.54e-17 71.6
Msa0062440 AT2G20400.1 67.347 49 16 0 20 68 233 281 1.01e-16 72.4
Msa0062440 AT2G20400.2 67.347 49 16 0 20 68 233 281 1.01e-16 72.4
Msa0062440 AT3G04450.1 65.306 49 17 0 20 68 240 288 1.20e-16 72.4
Msa0062440 AT5G29000.2 65.306 49 17 0 20 68 233 281 1.52e-16 72.0
Msa0062440 AT5G29000.1 65.306 49 17 0 20 68 190 238 1.76e-16 71.6
Msa0062440 AT5G29000.3 65.306 49 17 0 20 68 190 238 1.76e-16 71.6
Msa0062440 AT3G04450.4 65.306 49 17 0 20 68 200 248 1.81e-16 71.6
Msa0062440 AT3G04450.2 65.306 49 17 0 20 68 200 248 1.81e-16 71.6
Msa0062440 AT3G12730.1 64.000 50 18 0 19 68 24 73 2.15e-16 70.1
Msa0062440 AT4G13640.1 64.000 50 18 0 19 68 38 87 2.40e-16 70.9
Msa0062440 AT4G13640.2 64.000 50 18 0 19 68 38 87 2.56e-16 70.9
Msa0062440 AT3G24120.2 64.000 50 18 0 19 68 42 91 2.90e-16 70.5
Msa0062440 AT3G24120.1 64.000 50 18 0 19 68 42 91 2.92e-16 70.5
Msa0062440 AT2G06020.1 55.357 56 25 0 13 68 81 136 3.39e-16 70.5
Msa0062440 AT1G79430.2 64.000 50 18 0 19 68 35 84 5.31e-16 70.5
Msa0062440 AT3G13040.3 67.347 49 16 0 20 68 243 291 5.88e-16 70.5
Msa0062440 AT3G13040.1 67.347 49 16 0 20 68 243 291 5.88e-16 70.5
Msa0062440 AT3G13040.2 67.347 49 16 0 20 68 243 291 5.88e-16 70.5
Msa0062440 AT5G18240.2 58.000 50 21 0 19 68 46 95 1.70e-15 68.9
Msa0062440 AT5G18240.3 58.000 50 21 0 19 68 46 95 1.70e-15 68.9
Msa0062440 AT3G04030.1 56.000 50 22 0 19 68 46 95 1.93e-15 68.9
Msa0062440 AT5G18240.5 58.000 50 21 0 19 68 46 95 2.05e-15 68.9
Msa0062440 AT5G18240.1 58.000 50 21 0 19 68 46 95 2.27e-15 68.6
Msa0062440 AT5G18240.4 58.000 50 21 0 19 68 46 95 2.27e-15 68.6
Msa0062440 AT3G04030.3 56.000 50 22 0 19 68 46 95 2.62e-15 68.6
Msa0062440 AT2G01060.1 67.347 49 16 0 20 68 17 65 4.40e-15 67.4
Msa0062440 AT5G06800.3 61.224 49 19 0 20 68 194 242 1.69e-14 65.9
Msa0062440 AT1G69580.1 54.000 50 23 0 19 68 35 84 4.95e-14 64.7
Msa0062440 AT1G69580.2 54.000 50 23 0 19 68 35 84 4.97e-14 64.7
Msa0062440 AT5G06800.2 61.224 49 19 0 20 68 194 242 1.26e-13 63.9
Msa0062440 AT5G06800.1 61.224 49 19 0 20 68 194 242 1.48e-13 63.5
Msa0062440 AT4G37180.3 54.386 57 26 0 12 68 204 260 3.95e-13 62.0
Msa0062440 AT4G37180.1 55.357 56 25 0 13 68 205 260 4.01e-13 62.4
Msa0062440 AT4G37180.2 55.357 56 25 0 13 68 212 267 4.80e-13 62.0
Msa0062440 AT2G03500.1 60.870 46 18 0 23 68 238 283 1.24e-12 60.8
Msa0062440 AT3G04030.2 52.000 50 23 1 19 68 46 94 1.64e-12 60.5
Msa0062440 AT1G49190.1 47.541 61 29 1 11 68 208 268 2.19e-12 60.1
Msa0062440 AT1G49190.2 47.541 61 29 1 11 68 413 473 2.25e-12 60.1
Msa0062440 AT1G25550.1 58.696 46 19 0 23 68 214 259 2.78e-12 60.1
Msa0062440 AT3G62670.1 49.123 57 25 1 16 68 209 265 3.05e-12 59.7
Msa0062440 AT5G59570.2 54.717 53 23 1 16 68 139 190 4.26e-12 59.3
Msa0062440 AT5G59570.1 54.717 53 23 1 16 68 139 190 4.26e-12 59.3
Msa0062440 AT5G44190.1 55.769 52 22 1 17 68 146 196 4.27e-12 59.3
Msa0062440 AT5G44190.2 55.769 52 22 1 17 68 146 196 4.61e-12 59.3
Msa0062440 AT1G25550.2 50.000 58 29 0 11 68 202 259 6.75e-12 58.5
Msa0062440 AT3G10760.1 55.769 52 22 1 17 68 104 154 8.88e-12 58.5
Msa0062440 AT3G46640.2 53.846 52 23 1 17 68 143 193 3.02e-11 57.0
Msa0062440 AT3G46640.1 53.846 52 23 1 17 68 143 193 3.02e-11 57.0
Msa0062440 AT5G05090.1 55.769 52 22 1 17 68 80 130 4.01e-11 56.2
Msa0062440 AT3G46640.3 53.846 52 23 1 17 68 143 193 4.68e-11 56.6
Msa0062440 AT2G40970.1 55.769 52 22 1 17 68 104 154 5.26e-11 55.8
Msa0062440 AT1G68670.1 56.522 46 20 0 23 68 220 265 9.85e-11 55.5

Find 19 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCAGTTGATGGATGTTAA+AGG 0.259007 1_2:-21895184 Msa0062440:CDS
TAAGCTTGTTCTTCAGTTGA+TGG 0.287799 1_2:-21895194 Msa0062440:CDS
CTCATTCTTTCAAATCCAAA+AGG 0.369949 1_2:+21895469 None:intergenic
ACATGTTAAGAGCCATTTGC+AGG 0.373744 1_2:-21895149 None:intergenic
GTTTATGCCATTGAAACTCT+TGG 0.378805 1_2:-21895358 Msa0062440:CDS
TTCAGTTGATGGATGTTAAA+GGG 0.399431 1_2:-21895183 Msa0062440:CDS
ACAAAGCAACGATGAAGTTC+AGG 0.401903 1_2:+21895379 None:intergenic
CAAAGGTTCCTCGTTTAAGA+TGG 0.432536 1_2:-21895405 Msa0062440:CDS
ATGACCACCAAGAGTTTCAA+TGG 0.434104 1_2:+21895351 None:intergenic
TATCAAAATCAACCTGCAAA+TGG 0.495949 1_2:+21895137 None:intergenic
ATCAACTGAAGAACAAGCTT+AGG 0.508937 1_2:+21895196 None:intergenic
TCATTCTTTCAAATCCAAAA+GGG 0.518435 1_2:+21895470 None:intergenic
ATGAGTAGTTGTGGAAGGGA+AGG 0.528216 1_2:-21895451 Msa0062440:CDS
AAAGAATGAGTAGTTGTGGA+AGG 0.546541 1_2:-21895456 Msa0062440:exon
TTTGAAAGAATGAGTAGTTG+TGG 0.567741 1_2:-21895460 Msa0062440:exon
AAGAATGAGTAGTTGTGGAA+GGG 0.598417 1_2:-21895455 Msa0062440:exon
GAGACAATATGTAAGATCAA+AGG 0.602756 1_2:-21895422 Msa0062440:CDS
TATGCCATTGAAACTCTTGG+TGG 0.619663 1_2:-21895355 Msa0062440:CDS
TCAGGTGTCCATCTTAAACG+AGG 0.752182 1_2:+21895397 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCATTCATTTCTATTACTA+TGG - chr1_2:21895366-21895385 Msa0062440:CDS 20.0%
! TCATTCTTTCAAATCCAAAA+GGG + chr1_2:21895183-21895202 None:intergenic 25.0%
!!! TTTTTTTCCTTCATTTGTAG+AGG - chr1_2:21895426-21895445 Msa0062440:CDS 25.0%
CTCATTCTTTCAAATCCAAA+AGG + chr1_2:21895184-21895203 None:intergenic 30.0%
TTTGAAAGAATGAGTAGTTG+TGG - chr1_2:21895190-21895209 Msa0062440:CDS 30.0%
GAGACAATATGTAAGATCAA+AGG - chr1_2:21895228-21895247 Msa0062440:intron 30.0%
!! TTCAGTTGATGGATGTTAAA+GGG - chr1_2:21895467-21895486 Msa0062440:exon 30.0%
!!! GTGTTTTGAGGGTTTTTTTG+AGG - chr1_2:21895156-21895175 Msa0062440:CDS 35.0%
AAAGAATGAGTAGTTGTGGA+AGG - chr1_2:21895194-21895213 Msa0062440:CDS 35.0%
AAGAATGAGTAGTTGTGGAA+GGG - chr1_2:21895195-21895214 Msa0062440:CDS 35.0%
GTTTATGCCATTGAAACTCT+TGG - chr1_2:21895292-21895311 Msa0062440:intron 35.0%
ATCAACTGAAGAACAAGCTT+AGG + chr1_2:21895457-21895476 None:intergenic 35.0%
! TAAGCTTGTTCTTCAGTTGA+TGG - chr1_2:21895456-21895475 Msa0062440:exon 35.0%
!! CTTCAGTTGATGGATGTTAA+AGG - chr1_2:21895466-21895485 Msa0062440:exon 35.0%
!!! GGTTTTTTTGAGGACCCTTT+TGG - chr1_2:21895166-21895185 Msa0062440:CDS 40.0%
CAAAGGTTCCTCGTTTAAGA+TGG - chr1_2:21895245-21895264 Msa0062440:intron 40.0%
ACAAAGCAACGATGAAGTTC+AGG + chr1_2:21895274-21895293 None:intergenic 40.0%
TATGCCATTGAAACTCTTGG+TGG - chr1_2:21895295-21895314 Msa0062440:intron 40.0%
ATGACCACCAAGAGTTTCAA+TGG + chr1_2:21895302-21895321 None:intergenic 40.0%
ATGAGTAGTTGTGGAAGGGA+AGG - chr1_2:21895199-21895218 Msa0062440:CDS 45.0%
TCAGGTGTCCATCTTAAACG+AGG + chr1_2:21895256-21895275 None:intergenic 45.0%
GGAGTAGCCTCTACAAATGA+AGG + chr1_2:21895436-21895455 None:intergenic 45.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 21895150 21895522 21895150 ID=Msa0062440;Name=Msa0062440
chr1_2 mRNA 21895150 21895522 21895150 ID=Msa0062440-mRNA-1;Parent=Msa0062440;Name=Msa0062440-mRNA-1;_AED=0.00;_eAED=0.00;_QI=49|1|1|1|1|1|2|0|68
chr1_2 exon 21895150 21895226 21895150 ID=Msa0062440-mRNA-1:exon:8127;Parent=Msa0062440-mRNA-1
chr1_2 exon 21895347 21895522 21895347 ID=Msa0062440-mRNA-1:exon:8126;Parent=Msa0062440-mRNA-1
chr1_2 five_prime_UTR 21895474 21895522 21895474 ID=Msa0062440-mRNA-1:five_prime_utr;Parent=Msa0062440-mRNA-1
chr1_2 CDS 21895347 21895473 21895347 ID=Msa0062440-mRNA-1:cds;Parent=Msa0062440-mRNA-1
chr1_2 CDS 21895150 21895226 21895150 ID=Msa0062440-mRNA-1:cds;Parent=Msa0062440-mRNA-1
Gene Sequence

>Msa0062440

ATGAGTAGTTGTGGAAGGGAAGGTGTTGTGAGACAATATGTAAGATCAAAGGTTCCTCGTTTAAGATGGACACCTGAACTTCATCGTTGCTTTGTTTATGCCATTGAAACTCTTGGTGGTCATCAAAAGGCTACTCCTAAGCTTGTTCTTCAGTTGATGGATGTTAAAGGGTTAACAATTTCACATGTTAAGAGCCATTTGCAG

Protein sequence

>Msa0062440

MSSCGREGVVRQYVRSKVPRLRWTPELHRCFVYAIETLGGHQKATPKLVLQLMDVKGLTISHVKSHLQ