Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1435480 | AES72186.1 | 92.623 | 122 | 8 | 1 | 1 | 122 | 1 | 121 | 5.53e-74 | 226 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1435480 | sp|Q9LFT5|EPFL1_ARATH | 47.573 | 103 | 44 | 3 | 26 | 122 | 24 | 122 | 2.12e-24 | 93.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1435480 | G7J3Q1 | 92.623 | 122 | 8 | 1 | 1 | 122 | 1 | 121 | 2.64e-74 | 226 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0002930 | Msa1435480 | 0.800703 | 1.272348e-48 | -8.615850e-47 |
| Msa0015710 | Msa1435480 | 0.841716 | 4.013205e-58 | -8.615850e-47 |
| Msa0019600 | Msa1435480 | 0.807221 | 5.619345e-50 | -8.615850e-47 |
| Msa0036430 | Msa1435480 | 0.800335 | 1.512629e-48 | -8.615850e-47 |
| Msa0050040 | Msa1435480 | 0.800364 | 1.492041e-48 | -8.615850e-47 |
| Msa0055450 | Msa1435480 | 0.808479 | 3.035663e-50 | -8.615850e-47 |
| Msa0062440 | Msa1435480 | 0.820293 | 7.405317e-53 | -8.615850e-47 |
| Msa0074650 | Msa1435480 | 0.801656 | 8.122715e-49 | -8.615850e-47 |
| Msa0084500 | Msa1435480 | 0.824621 | 7.309464e-54 | -8.615850e-47 |
| Msa0102990 | Msa1435480 | 0.800002 | 1.767855e-48 | -8.615850e-47 |
| Msa0104500 | Msa1435480 | 0.832266 | 1.042969e-55 | -8.615850e-47 |
| Msa0109880 | Msa1435480 | 0.801847 | 7.423160e-49 | -8.615850e-47 |
| Msa1345420 | Msa1435480 | 0.882578 | 9.263077e-71 | -8.615850e-47 |
| Msa1379970 | Msa1435480 | 0.825684 | 4.099394e-54 | -8.615850e-47 |
| Msa1389050 | Msa1435480 | 0.851060 | 1.134740e-60 | -8.615850e-47 |
| Msa1394890 | Msa1435480 | 0.806388 | 8.426219e-50 | -8.615850e-47 |
| Msa1406780 | Msa1435480 | 0.825561 | 4.383642e-54 | -8.615850e-47 |
| Msa1420060 | Msa1435480 | 0.840547 | 8.140909e-58 | -8.615850e-47 |
| Msa1421550 | Msa1435480 | 0.843934 | 1.032455e-58 | -8.615850e-47 |
| Msa1431850 | Msa1435480 | 0.832641 | 8.417528e-56 | -8.615850e-47 |
| Msa1435480 | Msa1442630 | 0.819755 | 9.833748e-53 | -8.615850e-47 |
| Msa1435480 | Msa1456730 | 0.811113 | 8.233847e-51 | -8.615850e-47 |
| Msa1435480 | Msa1458910 | 0.947813 | 3.234948e-106 | -8.615850e-47 |
| Msa1435480 | Msa1459530 | 0.813175 | 2.923395e-51 | -8.615850e-47 |
| Msa1435480 | Msa1462530 | 0.837449 | 5.164282e-57 | -8.615850e-47 |
| Msa0639460 | Msa1435480 | 0.805663 | 1.197133e-49 | -8.615850e-47 |
| Msa0667850 | Msa1435480 | 0.831435 | 1.672113e-55 | -8.615850e-47 |
| Msa0669840 | Msa1435480 | 0.863930 | 1.751012e-64 | -8.615850e-47 |
| Msa0682360 | Msa1435480 | 0.804568 | 2.029378e-49 | -8.615850e-47 |
| Msa0691160 | Msa1435480 | 0.803616 | 3.202484e-49 | -8.615850e-47 |
| Msa0707220 | Msa1435480 | 0.815679 | 8.169822e-52 | -8.615850e-47 |
| Msa0725860 | Msa1435480 | 0.832776 | 7.794293e-56 | -8.615850e-47 |
| Msa0739580 | Msa1435480 | 0.819472 | 1.141109e-52 | -8.615850e-47 |
| Msa0742180 | Msa1435480 | 0.844459 | 7.463875e-59 | -8.615850e-47 |
| Msa0764820 | Msa1435480 | 0.802356 | 5.831531e-49 | -8.615850e-47 |
| Msa0778930 | Msa1435480 | 0.801666 | 8.084186e-49 | -8.615850e-47 |
| Msa0786040 | Msa1435480 | 0.829047 | 6.404294e-55 | -8.615850e-47 |
| Msa0819900 | Msa1435480 | 0.810921 | 9.064944e-51 | -8.615850e-47 |
| Msa0823400 | Msa1435480 | 0.872559 | 2.897807e-67 | -8.615850e-47 |
| Msa0828830 | Msa1435480 | 0.803464 | 3.443143e-49 | -8.615850e-47 |
| Msa0124130 | Msa1435480 | 0.831511 | 1.601849e-55 | -8.615850e-47 |
| Msa0130570 | Msa1435480 | 0.815417 | 9.344213e-52 | -8.615850e-47 |
| Msa0149140 | Msa1435480 | 0.816905 | 4.346413e-52 | -8.615850e-47 |
| Msa0152480 | Msa1435480 | 0.805220 | 1.482819e-49 | -8.615850e-47 |
| Msa0152500 | Msa1435480 | 0.812348 | 4.436482e-51 | -8.615850e-47 |
| Msa0153420 | Msa1435480 | 0.804671 | 1.931617e-49 | -8.615850e-47 |
| Msa0156250 | Msa1435480 | 0.828416 | 9.101545e-55 | -8.615850e-47 |
| Msa0176470 | Msa1435480 | 0.835773 | 1.380227e-56 | -8.615850e-47 |
| Msa0194380 | Msa1435480 | 0.885182 | 1.013770e-71 | -8.615850e-47 |
| Msa0200030 | Msa1435480 | 0.810660 | 1.031958e-50 | -8.615850e-47 |
| Msa0203030 | Msa1435480 | 0.841642 | 4.198747e-58 | -8.615850e-47 |
| Msa0206800 | Msa1435480 | 0.840608 | 7.847931e-58 | -8.615850e-47 |
| Msa0212150 | Msa1435480 | 0.823621 | 1.255048e-53 | -8.615850e-47 |
| Msa0219190 | Msa1435480 | 0.836858 | 7.312309e-57 | -8.615850e-47 |
| Msa0236110 | Msa1435480 | 0.841479 | 4.633897e-58 | -8.615850e-47 |
| Msa1228190 | Msa1435480 | 0.882500 | 9.885989e-71 | -8.615850e-47 |
| Msa1233560 | Msa1435480 | 0.818545 | 1.853884e-52 | -8.615850e-47 |
| Msa1243020 | Msa1435480 | 0.804585 | 2.013146e-49 | -8.615850e-47 |
| Msa1271260 | Msa1435480 | 0.882290 | 1.179533e-70 | -8.615850e-47 |
| Msa1292040 | Msa1435480 | 0.807218 | 5.627553e-50 | -8.615850e-47 |
| Msa1294290 | Msa1435480 | 0.836798 | 7.576536e-57 | -8.615850e-47 |
| Msa1305510 | Msa1435480 | 0.807677 | 4.498194e-50 | -8.615850e-47 |
| Msa1336620 | Msa1435480 | 0.802331 | 5.902188e-49 | -8.615850e-47 |
| Msa1339440 | Msa1435480 | 0.824087 | 9.761763e-54 | -8.615850e-47 |
| Msa0373960 | Msa1435480 | 0.920111 | 1.935135e-87 | -8.615850e-47 |
| Msa0382010 | Msa1435480 | 0.807887 | 4.058766e-50 | -8.615850e-47 |
| Msa0392420 | Msa1435480 | 0.802471 | 5.521825e-49 | -8.615850e-47 |
| Msa0406000 | Msa1435480 | 0.858466 | 8.078805e-63 | -8.615850e-47 |
| Msa0423100 | Msa1435480 | 0.956278 | 4.296883e-114 | -8.615850e-47 |
| Msa0424330 | Msa1435480 | 0.814778 | 1.295212e-51 | -8.615850e-47 |
| Msa0424340 | Msa1435480 | 0.827346 | 1.645708e-54 | -8.615850e-47 |
| Msa0436500 | Msa1435480 | 0.800520 | 1.386703e-48 | -8.615850e-47 |
| Msa0441590 | Msa1435480 | 0.825044 | 5.809333e-54 | -8.615850e-47 |
| Msa0451400 | Msa1435480 | 0.871228 | 8.018337e-67 | -8.615850e-47 |
| Msa0469260 | Msa1435480 | 0.955423 | 3.138439e-113 | -8.615850e-47 |
| Msa0996270 | Msa1435480 | 0.850674 | 1.457447e-60 | -8.615850e-47 |
| Msa0999300 | Msa1435480 | 0.828572 | 8.343315e-55 | -8.615850e-47 |
| Msa0999340 | Msa1435480 | 0.890609 | 8.436611e-74 | -8.615850e-47 |
| Msa0999350 | Msa1435480 | 0.879372 | 1.315043e-69 | -8.615850e-47 |
| Msa0999360 | Msa1435480 | 0.857471 | 1.596438e-62 | -8.615850e-47 |
| Msa0999390 | Msa1435480 | 0.884210 | 2.329214e-71 | -8.615850e-47 |
| Msa0999400 | Msa1435480 | 0.871208 | 8.141825e-67 | -8.615850e-47 |
| Msa0999410 | Msa1435480 | 0.860277 | 2.310972e-63 | -8.615850e-47 |
| Msa0999700 | Msa1435480 | 0.840530 | 8.228276e-58 | -8.615850e-47 |
| Msa1001890 | Msa1435480 | 0.811737 | 6.029126e-51 | -8.615850e-47 |
| Msa1033560 | Msa1435480 | 0.805862 | 1.087665e-49 | -8.615850e-47 |
| Msa1034680 | Msa1435480 | 0.819120 | 1.372014e-52 | -8.615850e-47 |
| Msa1037890 | Msa1435480 | 0.807455 | 5.011617e-50 | -8.615850e-47 |
| Msa1037900 | Msa1435480 | 0.806304 | 8.777905e-50 | -8.615850e-47 |
| Msa1042670 | Msa1435480 | 0.815702 | 8.074988e-52 | -8.615850e-47 |
| Msa1047990 | Msa1435480 | 0.815571 | 8.634876e-52 | -8.615850e-47 |
| Msa1060580 | Msa1435480 | 0.819429 | 1.167109e-52 | -8.615850e-47 |
| Msa1073340 | Msa1435480 | 0.853596 | 2.153205e-61 | -8.615850e-47 |
| Msa1079930 | Msa1435480 | 0.807299 | 5.410750e-50 | -8.615850e-47 |
| Msa1084520 | Msa1435480 | 0.824849 | 6.457687e-54 | -8.615850e-47 |
| Msa1092020 | Msa1435480 | 0.835310 | 1.807711e-56 | -8.615850e-47 |
| Msa1100140 | Msa1435480 | 0.803199 | 3.906795e-49 | -8.615850e-47 |
| Msa0252630 | Msa1435480 | 0.816840 | 4.495104e-52 | -8.615850e-47 |
| Msa0252770 | Msa1435480 | 0.828319 | 9.603691e-55 | -8.615850e-47 |
| Msa0273990 | Msa1435480 | 0.831287 | 1.817999e-55 | -8.615850e-47 |
| Msa0277290 | Msa1435480 | 0.841153 | 5.647906e-58 | -8.615850e-47 |
| Msa0280750 | Msa1435480 | 0.815977 | 7.010532e-52 | -8.615850e-47 |
| Msa0288190 | Msa1435480 | 0.832595 | 8.644734e-56 | -8.615850e-47 |
| Msa0288200 | Msa1435480 | 0.830247 | 3.269224e-55 | -8.615850e-47 |
| Msa0289920 | Msa1435480 | 0.802548 | 5.324803e-49 | -8.615850e-47 |
| Msa0291330 | Msa1435480 | 0.801948 | 7.076420e-49 | -8.615850e-47 |
| Msa0298240 | Msa1435480 | 0.842300 | 2.813482e-58 | -8.615850e-47 |
| Msa0306120 | Msa1435480 | 0.830735 | 2.484346e-55 | -8.615850e-47 |
| Msa0314630 | Msa1435480 | 0.851271 | 9.894204e-61 | -8.615850e-47 |
| Msa0322830 | Msa1435480 | 0.842886 | 1.966084e-58 | -8.615850e-47 |
| Msa0322840 | Msa1435480 | 0.850720 | 1.414646e-60 | -8.615850e-47 |
| Msa0322860 | Msa1435480 | 0.858186 | 9.790734e-63 | -8.615850e-47 |
| Msa0326540 | Msa1435480 | 0.826062 | 3.333516e-54 | -8.615850e-47 |
| Msa0329620 | Msa1435480 | 0.848392 | 6.311154e-60 | -8.615850e-47 |
| Msa0331530 | Msa1435480 | 0.839134 | 1.900586e-57 | -8.615850e-47 |
| Msa0331540 | Msa1435480 | 0.827189 | 1.794429e-54 | -8.615850e-47 |
| Msa0351770 | Msa1435480 | 0.840905 | 6.562224e-58 | -8.615850e-47 |
| Msa0359250 | Msa1435480 | 0.852550 | 4.290025e-61 | -8.615850e-47 |
| Msa1113850 | Msa1435480 | 0.834866 | 2.338869e-56 | -8.615850e-47 |
| Msa1125810 | Msa1435480 | 0.832702 | 8.130073e-56 | -8.615850e-47 |
| Msa1135380 | Msa1435480 | 0.844397 | 7.754303e-59 | -8.615850e-47 |
| Msa1145020 | Msa1435480 | 0.873890 | 1.035262e-67 | -8.615850e-47 |
| Msa1171310 | Msa1435480 | 0.832705 | 8.116358e-56 | -8.615850e-47 |
| Msa1177400 | Msa1435480 | 0.819040 | 1.430609e-52 | -8.615850e-47 |
| Msa1179360 | Msa1435480 | 0.822174 | 2.727608e-53 | -8.615850e-47 |
| Msa1179520 | Msa1435480 | 0.848376 | 6.375789e-60 | -8.615850e-47 |
| Msa1183430 | Msa1435480 | 0.821657 | 3.594006e-53 | -8.615850e-47 |
| Msa1201110 | Msa1435480 | 0.825154 | 5.472488e-54 | -8.615850e-47 |
| Msa1201160 | Msa1435480 | 0.848787 | 4.904425e-60 | -8.615850e-47 |
| Msa0487570 | Msa1435480 | 0.808736 | 2.675237e-50 | -8.615850e-47 |
| Msa0492190 | Msa1435480 | 0.843418 | 1.418862e-58 | -8.615850e-47 |
| Msa0514980 | Msa1435480 | 0.848559 | 5.673394e-60 | -8.615850e-47 |
| Msa0515950 | Msa1435480 | 0.816435 | 5.538035e-52 | -8.615850e-47 |
| Msa0520510 | Msa1435480 | 0.864312 | 1.331328e-64 | -8.615850e-47 |
| Msa0534930 | Msa1435480 | 0.830934 | 2.220516e-55 | -8.615850e-47 |
| Msa0535040 | Msa1435480 | 0.836401 | 9.563408e-57 | -8.615850e-47 |
| Msa0575740 | Msa1435480 | 0.864543 | 1.127183e-64 | -8.615850e-47 |
| Msa0591790 | Msa1435480 | 0.854150 | 1.491093e-61 | -8.615850e-47 |
| Msa0591800 | Msa1435480 | 0.874693 | 5.530833e-68 | -8.615850e-47 |
| Msa0845940 | Msa1435480 | 0.836712 | 7.968225e-57 | -8.615850e-47 |
| Msa0860170 | Msa1435480 | 0.833952 | 3.968227e-56 | -8.615850e-47 |
| Msa0860280 | Msa1435480 | 0.824841 | 6.488080e-54 | -8.615850e-47 |
| Msa0862310 | Msa1435480 | 0.850995 | 1.183557e-60 | -8.615850e-47 |
| Msa0867350 | Msa1435480 | 0.812524 | 4.060950e-51 | -8.615850e-47 |
| Msa0885060 | Msa1435480 | 0.821756 | 3.408419e-53 | -8.615850e-47 |
| Msa0898730 | Msa1435480 | 0.808616 | 2.837386e-50 | -8.615850e-47 |
| Msa0899950 | Msa1435480 | 0.840049 | 1.098793e-57 | -8.615850e-47 |
| Msa0922750 | Msa1435480 | 0.808960 | 2.395772e-50 | -8.615850e-47 |
| Msa0927120 | Msa1435480 | 0.813356 | 2.668611e-51 | -8.615850e-47 |
| Msa0929870 | Msa1435480 | 0.838876 | 2.216350e-57 | -8.615850e-47 |
| Msa0946210 | Msa1435480 | 0.822304 | 2.544920e-53 | -8.615850e-47 |
| Msa0961560 | Msa1435480 | 0.836268 | 1.033547e-56 | -8.615850e-47 |
| Msa0965310 | Msa1435480 | 0.857284 | 1.812835e-62 | -8.615850e-47 |
| Msa0979510 | Msa1435480 | 0.835563 | 1.560472e-56 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1435480 | MtrunA17_Chr3g0123851 | 92.623 | 122 | 8 | 1 | 1 | 122 | 1 | 121 | 5.08e-78 | 226 |
| Msa1435480 | MtrunA17_Chr8g0381891 | 57.143 | 126 | 47 | 3 | 1 | 122 | 1 | 123 | 8.39e-35 | 116 |
| Msa1435480 | MtrunA17_Chr3g0140691 | 41.860 | 86 | 38 | 2 | 40 | 122 | 22 | 98 | 3.68e-16 | 68.6 |
| Msa1435480 | MtrunA17_Chr4g0054461 | 42.857 | 77 | 39 | 2 | 46 | 122 | 31 | 102 | 2.87e-14 | 63.5 |
| Msa1435480 | MtrunA17_Chr2g0330071 | 42.105 | 76 | 27 | 2 | 47 | 122 | 70 | 128 | 8.73e-14 | 63.2 |
| Msa1435480 | MtrunA17_Chr4g0063871 | 38.824 | 85 | 38 | 2 | 39 | 122 | 43 | 114 | 3.18e-13 | 61.2 |
| Msa1435480 | MtrunA17_Chr2g0333071 | 34.146 | 123 | 69 | 5 | 1 | 122 | 2 | 113 | 1.19e-12 | 59.7 |
| Msa1435480 | MtrunA17_Chr5g0400761 | 37.662 | 77 | 43 | 2 | 46 | 122 | 60 | 131 | 3.68e-12 | 58.9 |
| Msa1435480 | MtrunA17_Chr8g0342581 | 37.975 | 79 | 28 | 2 | 44 | 122 | 72 | 129 | 3.92e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1435480 | AT5G10310.1 | 47.573 | 103 | 44 | 3 | 26 | 122 | 24 | 122 | 2.15e-25 | 93.2 |
| Msa1435480 | AT5G10310.2 | 48.837 | 86 | 35 | 2 | 42 | 122 | 47 | 128 | 4.66e-23 | 87.4 |
Find 46 sgRNAs with CRISPR-Local
Find 154 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATATAACCTCATCTTGTTAA+TGG | 0.240049 | tig0027706:-6131 | Msa1435480:CDS |
| AAGAAACAATGAAATTTCTA+AGG | 0.259527 | tig0027706:+5495 | None:intergenic |
| TGTAGTGGAGGCTGAGATAG+AGG | 0.383685 | tig0027706:+6458 | None:intergenic |
| ATCCGTTATTATTATTACCT+TGG | 0.417680 | tig0027706:+5574 | None:intergenic |
| GGTTGATAGAAGCAAGAAAC+TGG | 0.429204 | tig0027706:+6076 | None:intergenic |
| GGCTGAACATGTTCATGGCT+TGG | 0.438174 | tig0027706:+5645 | None:intergenic |
| GACCTGGCTGAACATGTTCA+TGG | 0.454543 | tig0027706:+5640 | None:intergenic |
| CTCCAAGGTAATAATAATAA+CGG | 0.464837 | tig0027706:-5576 | Msa1435480:CDS |
| TGTGTAGGGTTTATTATTTG+AGG | 0.467998 | tig0027706:-5748 | Msa1435480:intron |
| AAGAAACTGGACACAGTAGA+AGG | 0.491502 | tig0027706:+6089 | None:intergenic |
| CAATGGCATTTCCAACTCAA+TGG | 0.495776 | tig0027706:+5537 | None:intergenic |
| AAAGAAATTACCACCTTCCA+TGG | 0.501489 | tig0027706:+5599 | None:intergenic |
| GGAACTTGCACTGCCATGCA+TGG | 0.505648 | tig0027706:+5666 | None:intergenic |
| CACTGCCTCAACTTCATCCA+TGG | 0.509432 | tig0027706:-5616 | Msa1435480:CDS |
| AGGGAAAAGCCTACCCTTGG+AGG | 0.512819 | tig0027706:+6040 | None:intergenic |
| ATTTCTAAGGGTTGAAGAAG+TGG | 0.515036 | tig0027706:+5508 | None:intergenic |
| GGAGAGGGATAGTGTAGTGG+AGG | 0.517083 | tig0027706:+6446 | None:intergenic |
| CAAATTACAAGCCATTGAGT+TGG | 0.518087 | tig0027706:-5548 | Msa1435480:CDS |
| GGTAACAATTGTGTAGAGAA+AGG | 0.520211 | tig0027706:+6425 | None:intergenic |
| AAAGGAGAGGGATAGTGTAG+TGG | 0.532448 | tig0027706:+6443 | None:intergenic |
| GGTGGTAATTTCTTTCTCCA+AGG | 0.542564 | tig0027706:-5591 | Msa1435480:CDS |
| CACATTCTGCAATTCCTCCA+AGG | 0.543179 | tig0027706:-6054 | Msa1435480:CDS |
| AGAAACAATGAAATTTCTAA+GGG | 0.551654 | tig0027706:+5496 | None:intergenic |
| AGATAGAGGGTGCAGAGTGT+TGG | 0.551815 | tig0027706:+6472 | None:intergenic |
| AGCCATGAACATGTTCAGCC+AGG | 0.557195 | tig0027706:-5642 | Msa1435480:CDS |
| GAAGTTGAGGCAGTGTGACC+TGG | 0.560053 | tig0027706:+5624 | None:intergenic |
| AATTGTGTAGAGAAAGGAGA+GGG | 0.568206 | tig0027706:+6431 | None:intergenic |
| ACCTTCCATGGATGAAGTTG+AGG | 0.576895 | tig0027706:+5611 | None:intergenic |
| GCCTCAACTTCATCCATGGA+AGG | 0.583985 | tig0027706:-5612 | Msa1435480:CDS |
| ATATGAATTAAATGAAGCCA+TGG | 0.598193 | tig0027706:+6150 | None:intergenic |
| TTACATTTGTTGTGACATGT+AGG | 0.598733 | tig0027706:+5699 | None:intergenic |
| GTAGTGGAGGCTGAGATAGA+GGG | 0.606138 | tig0027706:+6459 | None:intergenic |
| TGATGACCATTAACAAGATG+AGG | 0.607087 | tig0027706:+6125 | None:intergenic |
| CATTTGTTGTGACATGTAGG+TGG | 0.607908 | tig0027706:+5702 | None:intergenic |
| TCAGCAGTGATTGTATACAT+TGG | 0.616295 | tig0027706:-5368 | Msa1435480:three_prime_UTR |
| TTGAGTTGGAAATGCCATTG+TGG | 0.619909 | tig0027706:-5534 | Msa1435480:CDS |
| TGAAGAAGTGGCCACCACAA+TGG | 0.621299 | tig0027706:+5520 | None:intergenic |
| TAACCAATGTCATCCATGCA+TGG | 0.626151 | tig0027706:-5679 | Msa1435480:CDS |
| ACATTCTGCAATTCCTCCAA+GGG | 0.630675 | tig0027706:-6053 | Msa1435480:intron |
| ACATGATAGATAGATATCCA+TGG | 0.649649 | tig0027706:-6167 | Msa1435480:exon |
| AGTGTTGGCAGAAAAGTGAA+AGG | 0.656008 | tig0027706:+6487 | None:intergenic |
| TCAACTTCATCCATGGAAGG+TGG | 0.660618 | tig0027706:-5609 | Msa1435480:CDS |
| CTGCCATGCATGGATGACAT+TGG | 0.662535 | tig0027706:+5676 | None:intergenic |
| CTTGGAGGAATTGCAGAATG+TGG | 0.672231 | tig0027706:+6055 | None:intergenic |
| AGTTGGAAATGCCATTGTGG+TGG | 0.687706 | tig0027706:-5531 | Msa1435480:CDS |
| CAATTGTGTAGAGAAAGGAG+AGG | 0.717317 | tig0027706:+6430 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTTGAATTCACTATAAAAT+TGG | + | tig0027706:5980-5999 | None:intergenic | 20.0% |
| !! | AGTTAACAAACTTAATTAAC+TGG | + | tig0027706:6055-6074 | None:intergenic | 20.0% |
| !! | AGAAACAATGAAATTTCTAA+GGG | + | tig0027706:6333-6352 | None:intergenic | 20.0% |
| !! | AAGAAACAATGAAATTTCTA+AGG | + | tig0027706:6334-6353 | None:intergenic | 20.0% |
| !! | GTTTGAATTCACTATAAAAT+TGG | + | tig0027706:5980-5999 | None:intergenic | 20.0% |
| !! | AGTTAACAAACTTAATTAAC+TGG | + | tig0027706:6055-6074 | None:intergenic | 20.0% |
| !! | AGAAACAATGAAATTTCTAA+GGG | + | tig0027706:6333-6352 | None:intergenic | 20.0% |
| !! | AAGAAACAATGAAATTTCTA+AGG | + | tig0027706:6334-6353 | None:intergenic | 20.0% |
| !!! | GTTTTTTTCTAGTTATGTGT+TGG | + | tig0027706:5429-5448 | None:intergenic | 25.0% |
| !! | AATGACAAACTTTTCATTCT+TGG | - | tig0027706:5473-5492 | Msa1435480:three_prime_UTR | 25.0% |
| !!! | TTATTTTGTCACATTACAAC+TGG | - | tig0027706:5544-5563 | Msa1435480:CDS | 25.0% |
| ! | ATATGAATTAAATGAAGCCA+TGG | + | tig0027706:5679-5698 | None:intergenic | 25.0% |
| ! | ATATAACCTCATCTTGTTAA+TGG | - | tig0027706:5695-5714 | Msa1435480:CDS | 25.0% |
| !!! | TAGTTGGTTTTAAAAGGAAT+CGG | - | tig0027706:5856-5875 | Msa1435480:intron | 25.0% |
| !! | ACAGTATATTACTGTGATTT+TGG | - | tig0027706:5914-5933 | Msa1435480:intron | 25.0% |
| ! | AATTCACTATAAAATTGGCT+CGG | + | tig0027706:5975-5994 | None:intergenic | 25.0% |
| ! | TATTATTTGAGGAGAAAAAC+AGG | - | tig0027706:6089-6108 | Msa1435480:CDS | 25.0% |
| ! | CTCCAAGGTAATAATAATAA+CGG | - | tig0027706:6250-6269 | Msa1435480:five_prime_UTR | 25.0% |
| ! | ATCCGTTATTATTATTACCT+TGG | + | tig0027706:6255-6274 | None:intergenic | 25.0% |
| !!! | GTTTTTTTCTAGTTATGTGT+TGG | + | tig0027706:5429-5448 | None:intergenic | 25.0% |
| !! | AATGACAAACTTTTCATTCT+TGG | - | tig0027706:5473-5492 | Msa1435480:three_prime_UTR | 25.0% |
| !!! | TTATTTTGTCACATTACAAC+TGG | - | tig0027706:5544-5563 | Msa1435480:CDS | 25.0% |
| ! | ATATGAATTAAATGAAGCCA+TGG | + | tig0027706:5679-5698 | None:intergenic | 25.0% |
| ! | ATATAACCTCATCTTGTTAA+TGG | - | tig0027706:5695-5714 | Msa1435480:CDS | 25.0% |
| !!! | TAGTTGGTTTTAAAAGGAAT+CGG | - | tig0027706:5856-5875 | Msa1435480:intron | 25.0% |
| !! | ACAGTATATTACTGTGATTT+TGG | - | tig0027706:5914-5933 | Msa1435480:intron | 25.0% |
| ! | AATTCACTATAAAATTGGCT+CGG | + | tig0027706:5975-5994 | None:intergenic | 25.0% |
| ! | TATTATTTGAGGAGAAAAAC+AGG | - | tig0027706:6089-6108 | Msa1435480:CDS | 25.0% |
| ! | CTCCAAGGTAATAATAATAA+CGG | - | tig0027706:6250-6269 | Msa1435480:five_prime_UTR | 25.0% |
| ! | ATCCGTTATTATTATTACCT+TGG | + | tig0027706:6255-6274 | None:intergenic | 25.0% |
| !!! | ATCTAGTTTTGAGCAATGAA+TGG | + | tig0027706:5451-5470 | None:intergenic | 30.0% |
| ACATGATAGATAGATATCCA+TGG | - | tig0027706:5659-5678 | Msa1435480:CDS | 30.0% | |
| TTATGTGTATGTTGCATAGT+TGG | - | tig0027706:5840-5859 | Msa1435480:intron | 30.0% | |
| !!! | GTTGCATAGTTGGTTTTAAA+AGG | - | tig0027706:5850-5869 | Msa1435480:intron | 30.0% |
| ACAGTAATATACTGTGATTC+TGG | + | tig0027706:5909-5928 | None:intergenic | 30.0% | |
| ! | ATCCTCATTTTGTTGAAACT+TGG | + | tig0027706:6008-6027 | None:intergenic | 30.0% |
| ACTTAATTAACTGGTGAGTT+TGG | + | tig0027706:6046-6065 | None:intergenic | 30.0% | |
| !! | AGTTTGTTAACTATGTGTGT+AGG | - | tig0027706:6063-6082 | Msa1435480:CDS | 30.0% |
| ! | GTTTGTTAACTATGTGTGTA+GGG | - | tig0027706:6064-6083 | Msa1435480:CDS | 30.0% |
| TGTGTAGGGTTTATTATTTG+AGG | - | tig0027706:6078-6097 | Msa1435480:CDS | 30.0% | |
| !! | TTACATTTGTTGTGACATGT+AGG | + | tig0027706:6130-6149 | None:intergenic | 30.0% |
| !!! | ATCTAGTTTTGAGCAATGAA+TGG | + | tig0027706:5451-5470 | None:intergenic | 30.0% |
| ACATGATAGATAGATATCCA+TGG | - | tig0027706:5659-5678 | Msa1435480:CDS | 30.0% | |
| TTATGTGTATGTTGCATAGT+TGG | - | tig0027706:5840-5859 | Msa1435480:intron | 30.0% | |
| !!! | GTTGCATAGTTGGTTTTAAA+AGG | - | tig0027706:5850-5869 | Msa1435480:intron | 30.0% |
| ACAGTAATATACTGTGATTC+TGG | + | tig0027706:5909-5928 | None:intergenic | 30.0% | |
| ! | ATCCTCATTTTGTTGAAACT+TGG | + | tig0027706:6008-6027 | None:intergenic | 30.0% |
| ACTTAATTAACTGGTGAGTT+TGG | + | tig0027706:6046-6065 | None:intergenic | 30.0% | |
| !! | AGTTTGTTAACTATGTGTGT+AGG | - | tig0027706:6063-6082 | Msa1435480:CDS | 30.0% |
| ! | GTTTGTTAACTATGTGTGTA+GGG | - | tig0027706:6064-6083 | Msa1435480:CDS | 30.0% |
| TGTGTAGGGTTTATTATTTG+AGG | - | tig0027706:6078-6097 | Msa1435480:CDS | 30.0% | |
| !! | TTACATTTGTTGTGACATGT+AGG | + | tig0027706:6130-6149 | None:intergenic | 30.0% |
| AATTGTGTAGAGAAAGGAGA+GGG | + | tig0027706:5398-5417 | None:intergenic | 35.0% | |
| GGTAACAATTGTGTAGAGAA+AGG | + | tig0027706:5404-5423 | None:intergenic | 35.0% | |
| !!! | TTTTCTAGTTATGTGTTGGC+AGG | + | tig0027706:5425-5444 | None:intergenic | 35.0% |
| TGATGACCATTAACAAGATG+AGG | + | tig0027706:5704-5723 | None:intergenic | 35.0% | |
| AGTGAATGAGGGAGAATAAA+GGG | + | tig0027706:5808-5827 | None:intergenic | 35.0% | |
| GCAACATACACATAAAGCAA+AGG | + | tig0027706:5835-5854 | None:intergenic | 35.0% | |
| CACCAAGTTTCAACAAAATG+AGG | - | tig0027706:6003-6022 | Msa1435480:intron | 35.0% | |
| TTTGAGGAGAAAAACAGGTT+AGG | - | tig0027706:6094-6113 | Msa1435480:CDS | 35.0% | |
| AAAGAAATTACCACCTTCCA+TGG | + | tig0027706:6230-6249 | None:intergenic | 35.0% | |
| CAAATTACAAGCCATTGAGT+TGG | - | tig0027706:6278-6297 | Msa1435480:five_prime_UTR | 35.0% | |
| ! | ATTTCTAAGGGTTGAAGAAG+TGG | + | tig0027706:6321-6340 | None:intergenic | 35.0% |
| TCAGCAGTGATTGTATACAT+TGG | - | tig0027706:6458-6477 | Msa1435480:five_prime_UTR | 35.0% | |
| AATTGTGTAGAGAAAGGAGA+GGG | + | tig0027706:5398-5417 | None:intergenic | 35.0% | |
| GGTAACAATTGTGTAGAGAA+AGG | + | tig0027706:5404-5423 | None:intergenic | 35.0% | |
| !!! | TTTTCTAGTTATGTGTTGGC+AGG | + | tig0027706:5425-5444 | None:intergenic | 35.0% |
| TGATGACCATTAACAAGATG+AGG | + | tig0027706:5704-5723 | None:intergenic | 35.0% | |
| AGTGAATGAGGGAGAATAAA+GGG | + | tig0027706:5808-5827 | None:intergenic | 35.0% | |
| GCAACATACACATAAAGCAA+AGG | + | tig0027706:5835-5854 | None:intergenic | 35.0% | |
| CACCAAGTTTCAACAAAATG+AGG | - | tig0027706:6003-6022 | Msa1435480:intron | 35.0% | |
| TTTGAGGAGAAAAACAGGTT+AGG | - | tig0027706:6094-6113 | Msa1435480:CDS | 35.0% | |
| AAAGAAATTACCACCTTCCA+TGG | + | tig0027706:6230-6249 | None:intergenic | 35.0% | |
| CAAATTACAAGCCATTGAGT+TGG | - | tig0027706:6278-6297 | Msa1435480:five_prime_UTR | 35.0% | |
| ! | ATTTCTAAGGGTTGAAGAAG+TGG | + | tig0027706:6321-6340 | None:intergenic | 35.0% |
| TCAGCAGTGATTGTATACAT+TGG | - | tig0027706:6458-6477 | Msa1435480:five_prime_UTR | 35.0% | |
| CAATTGTGTAGAGAAAGGAG+AGG | + | tig0027706:5399-5418 | None:intergenic | 40.0% | |
| AAGAAACTGGACACAGTAGA+AGG | + | tig0027706:5740-5759 | None:intergenic | 40.0% | |
| !! | GGTTGATAGAAGCAAGAAAC+TGG | + | tig0027706:5753-5772 | None:intergenic | 40.0% |
| ACATTCTGCAATTCCTCCAA+GGG | - | tig0027706:5773-5792 | Msa1435480:intron | 40.0% | |
| GAGTGAATGAGGGAGAATAA+AGG | + | tig0027706:5809-5828 | None:intergenic | 40.0% | |
| GCAAAGGAAAGAGTGAATGA+GGG | + | tig0027706:5819-5838 | None:intergenic | 40.0% | |
| AGCAAAGGAAAGAGTGAATG+AGG | + | tig0027706:5820-5839 | None:intergenic | 40.0% | |
| TGGTGAGTTTGGCAAAATCA+AGG | + | tig0027706:6035-6054 | None:intergenic | 40.0% | |
| !! | CATTTGTTGTGACATGTAGG+TGG | + | tig0027706:6127-6146 | None:intergenic | 40.0% |
| TAACCAATGTCATCCATGCA+TGG | - | tig0027706:6147-6166 | Msa1435480:CDS | 40.0% | |
| GGTGGTAATTTCTTTCTCCA+AGG | - | tig0027706:6235-6254 | Msa1435480:five_prime_UTR | 40.0% | |
| CAATGGCATTTCCAACTCAA+TGG | + | tig0027706:6292-6311 | None:intergenic | 40.0% | |
| !! | TTGAGTTGGAAATGCCATTG+TGG | - | tig0027706:6292-6311 | Msa1435480:five_prime_UTR | 40.0% |
| CAATTGTGTAGAGAAAGGAG+AGG | + | tig0027706:5399-5418 | None:intergenic | 40.0% | |
| AAGAAACTGGACACAGTAGA+AGG | + | tig0027706:5740-5759 | None:intergenic | 40.0% | |
| !! | GGTTGATAGAAGCAAGAAAC+TGG | + | tig0027706:5753-5772 | None:intergenic | 40.0% |
| ACATTCTGCAATTCCTCCAA+GGG | - | tig0027706:5773-5792 | Msa1435480:intron | 40.0% | |
| GAGTGAATGAGGGAGAATAA+AGG | + | tig0027706:5809-5828 | None:intergenic | 40.0% | |
| GCAAAGGAAAGAGTGAATGA+GGG | + | tig0027706:5819-5838 | None:intergenic | 40.0% | |
| AGCAAAGGAAAGAGTGAATG+AGG | + | tig0027706:5820-5839 | None:intergenic | 40.0% | |
| TGGTGAGTTTGGCAAAATCA+AGG | + | tig0027706:6035-6054 | None:intergenic | 40.0% | |
| !! | CATTTGTTGTGACATGTAGG+TGG | + | tig0027706:6127-6146 | None:intergenic | 40.0% |
| TAACCAATGTCATCCATGCA+TGG | - | tig0027706:6147-6166 | Msa1435480:CDS | 40.0% | |
| GGTGGTAATTTCTTTCTCCA+AGG | - | tig0027706:6235-6254 | Msa1435480:five_prime_UTR | 40.0% | |
| CAATGGCATTTCCAACTCAA+TGG | + | tig0027706:6292-6311 | None:intergenic | 40.0% | |
| !! | TTGAGTTGGAAATGCCATTG+TGG | - | tig0027706:6292-6311 | Msa1435480:five_prime_UTR | 40.0% |
| AAAGGAGAGGGATAGTGTAG+TGG | + | tig0027706:5386-5405 | None:intergenic | 45.0% | |
| CTTGGAGGAATTGCAGAATG+TGG | + | tig0027706:5774-5793 | None:intergenic | 45.0% | |
| CACATTCTGCAATTCCTCCA+AGG | - | tig0027706:5772-5791 | Msa1435480:intron | 45.0% | |
| TAAAGGGAAAAGCCTACCCT+TGG | + | tig0027706:5792-5811 | None:intergenic | 45.0% | |
| GGAATCGGTGCATGTTTGAA+TGG | - | tig0027706:5871-5890 | Msa1435480:intron | 45.0% | |
| TCGGTGCATGTTTGAATGGA+CGG | - | tig0027706:5875-5894 | Msa1435480:intron | 45.0% | |
| GCTCGGAGCTTCAACAAAAT+CGG | + | tig0027706:5958-5977 | None:intergenic | 45.0% | |
| ACCTTCCATGGATGAAGTTG+AGG | + | tig0027706:6218-6237 | None:intergenic | 45.0% | |
| TCAACTTCATCCATGGAAGG+TGG | - | tig0027706:6217-6236 | Msa1435480:five_prime_UTR | 45.0% | |
| AGTTGGAAATGCCATTGTGG+TGG | - | tig0027706:6295-6314 | Msa1435480:five_prime_UTR | 45.0% | |
| AAAGGAGAGGGATAGTGTAG+TGG | + | tig0027706:5386-5405 | None:intergenic | 45.0% | |
| CTTGGAGGAATTGCAGAATG+TGG | + | tig0027706:5774-5793 | None:intergenic | 45.0% | |
| CACATTCTGCAATTCCTCCA+AGG | - | tig0027706:5772-5791 | Msa1435480:intron | 45.0% | |
| TAAAGGGAAAAGCCTACCCT+TGG | + | tig0027706:5792-5811 | None:intergenic | 45.0% | |
| GGAATCGGTGCATGTTTGAA+TGG | - | tig0027706:5871-5890 | Msa1435480:intron | 45.0% | |
| TCGGTGCATGTTTGAATGGA+CGG | - | tig0027706:5875-5894 | Msa1435480:intron | 45.0% | |
| GCTCGGAGCTTCAACAAAAT+CGG | + | tig0027706:5958-5977 | None:intergenic | 45.0% | |
| ACCTTCCATGGATGAAGTTG+AGG | + | tig0027706:6218-6237 | None:intergenic | 45.0% | |
| TCAACTTCATCCATGGAAGG+TGG | - | tig0027706:6217-6236 | Msa1435480:five_prime_UTR | 45.0% | |
| AGTTGGAAATGCCATTGTGG+TGG | - | tig0027706:6295-6314 | Msa1435480:five_prime_UTR | 45.0% | |
| !! | TAAATAAATAAAAACAAAAA+AGG | + | tig0027706:6415-6434 | None:intergenic | 5.0% |
| !! | TAAATAAATAAAAACAAAAA+AGG | + | tig0027706:6415-6434 | None:intergenic | 5.0% |
| ! | AGATAGAGGGTGCAGAGTGT+TGG | + | tig0027706:5357-5376 | None:intergenic | 50.0% |
| ! | GTAGTGGAGGCTGAGATAGA+GGG | + | tig0027706:5370-5389 | None:intergenic | 50.0% |
| ! | TGTAGTGGAGGCTGAGATAG+AGG | + | tig0027706:5371-5390 | None:intergenic | 50.0% |
| TCTGCAATTCCTCCAAGGGT+AGG | - | tig0027706:5777-5796 | Msa1435480:intron | 50.0% | |
| CTGCCATGCATGGATGACAT+TGG | + | tig0027706:6153-6172 | None:intergenic | 50.0% | |
| GGCTGAACATGTTCATGGCT+TGG | + | tig0027706:6184-6203 | None:intergenic | 50.0% | |
| AGCCATGAACATGTTCAGCC+AGG | - | tig0027706:6184-6203 | Msa1435480:five_prime_UTR | 50.0% | |
| GACCTGGCTGAACATGTTCA+TGG | + | tig0027706:6189-6208 | None:intergenic | 50.0% | |
| CACTGCCTCAACTTCATCCA+TGG | - | tig0027706:6210-6229 | Msa1435480:five_prime_UTR | 50.0% | |
| GCCTCAACTTCATCCATGGA+AGG | - | tig0027706:6214-6233 | Msa1435480:five_prime_UTR | 50.0% | |
| TGAAGAAGTGGCCACCACAA+TGG | + | tig0027706:6309-6328 | None:intergenic | 50.0% | |
| ! | AGATAGAGGGTGCAGAGTGT+TGG | + | tig0027706:5357-5376 | None:intergenic | 50.0% |
| ! | GTAGTGGAGGCTGAGATAGA+GGG | + | tig0027706:5370-5389 | None:intergenic | 50.0% |
| ! | TGTAGTGGAGGCTGAGATAG+AGG | + | tig0027706:5371-5390 | None:intergenic | 50.0% |
| TCTGCAATTCCTCCAAGGGT+AGG | - | tig0027706:5777-5796 | Msa1435480:intron | 50.0% | |
| CTGCCATGCATGGATGACAT+TGG | + | tig0027706:6153-6172 | None:intergenic | 50.0% | |
| GGCTGAACATGTTCATGGCT+TGG | + | tig0027706:6184-6203 | None:intergenic | 50.0% | |
| AGCCATGAACATGTTCAGCC+AGG | - | tig0027706:6184-6203 | Msa1435480:five_prime_UTR | 50.0% | |
| GACCTGGCTGAACATGTTCA+TGG | + | tig0027706:6189-6208 | None:intergenic | 50.0% | |
| CACTGCCTCAACTTCATCCA+TGG | - | tig0027706:6210-6229 | Msa1435480:five_prime_UTR | 50.0% | |
| GCCTCAACTTCATCCATGGA+AGG | - | tig0027706:6214-6233 | Msa1435480:five_prime_UTR | 50.0% | |
| TGAAGAAGTGGCCACCACAA+TGG | + | tig0027706:6309-6328 | None:intergenic | 50.0% | |
| GGAGAGGGATAGTGTAGTGG+AGG | + | tig0027706:5383-5402 | None:intergenic | 55.0% | |
| AGGGAAAAGCCTACCCTTGG+AGG | + | tig0027706:5789-5808 | None:intergenic | 55.0% | |
| GGAACTTGCACTGCCATGCA+TGG | + | tig0027706:6163-6182 | None:intergenic | 55.0% | |
| GAAGTTGAGGCAGTGTGACC+TGG | + | tig0027706:6205-6224 | None:intergenic | 55.0% | |
| GGAGAGGGATAGTGTAGTGG+AGG | + | tig0027706:5383-5402 | None:intergenic | 55.0% | |
| AGGGAAAAGCCTACCCTTGG+AGG | + | tig0027706:5789-5808 | None:intergenic | 55.0% | |
| GGAACTTGCACTGCCATGCA+TGG | + | tig0027706:6163-6182 | None:intergenic | 55.0% | |
| GAAGTTGAGGCAGTGTGACC+TGG | + | tig0027706:6205-6224 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0027706 | gene | 5341 | 6507 | 5341 | ID=Msa1435480;Name=Msa1435480 |
| tig0027706 | mRNA | 5341 | 6507 | 5341 | ID=Msa1435480-mRNA-1;Parent=Msa1435480;Name=Msa1435480-mRNA-1;_AED=0.00;_eAED=0.00;_QI=337|1|1|1|1|1|2|171|122 |
| tig0027706 | exon | 5341 | 5763 | 5341 | ID=Msa1435480-mRNA-1:exon:9725;Parent=Msa1435480-mRNA-1 |
| tig0027706 | exon | 6054 | 6507 | 6054 | ID=Msa1435480-mRNA-1:exon:9724;Parent=Msa1435480-mRNA-1 |
| tig0027706 | five_prime_UTR | 6171 | 6507 | 6171 | ID=Msa1435480-mRNA-1:five_prime_utr;Parent=Msa1435480-mRNA-1 |
| tig0027706 | CDS | 6054 | 6170 | 6054 | ID=Msa1435480-mRNA-1:cds;Parent=Msa1435480-mRNA-1 |
| tig0027706 | CDS | 5512 | 5763 | 5512 | ID=Msa1435480-mRNA-1:cds;Parent=Msa1435480-mRNA-1 |
| tig0027706 | three_prime_UTR | 5341 | 5511 | 5341 | ID=Msa1435480-mRNA-1:three_prime_utr;Parent=Msa1435480-mRNA-1 |
| Gene Sequence |
| Protein sequence |
