AlfalfaGEDB | Msa0149140

M. sativa cultivar zhongmu-4 / Msa0149140

KEGG: K11816
GO: GO:0000003 GO:0000139 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005783 GO:0005789 GO:0005794 GO:0005829 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009790 GO:0009791 GO:0009793 GO:0009850 GO:0009851 GO:0009909 GO:0009911 GO:0009987 GO:0010154 GO:0010229 GO:0010817 GO:0012505 GO:0016020 GO:0016829 GO:0016830 GO:0016831 GO:0022414 GO:0031090 GO:0031974 GO:0031981 GO:0031984 GO:0032501 GO:0032502 GO:0042175 GO:0042445 GO:0042446 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044249 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044431 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0047434 GO:0048316 GO:0048366 GO:0048367 GO:0048518 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048825 GO:0048827 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0051094 GO:0051239 GO:0051240 GO:0061458 GO:0065007 GO:0065008 GO:0070013 GO:0090567 GO:0098588 GO:0098791 GO:0098827 GO:0099402 GO:2000026 GO:2000241 GO:2000243
PFAM: PF00743.22

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0149140	XP_003588697.1	98.030	406	8	0	1	406	1	406	0.0	835

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0149140	sp\|Q9LFM5\|YUC4_ARATH	71.788	397	106	2	11	403	12	406	0.0	599

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0149140	G7I6C3	98.030	406	8	0	1	406	1	406	0.0	835

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0006980	Msa0149140	0.951154	3.710698e-109	-8.615850e-47
Msa0015710	Msa0149140	0.841809	3.792822e-58	-8.615850e-47
Msa0016120	Msa0149140	0.829189	5.915337e-55	-8.615850e-47
Msa0019600	Msa0149140	0.840159	1.028652e-57	-8.615850e-47
Msa0050040	Msa0149140	0.823804	1.137300e-53	-8.615850e-47
Msa0055450	Msa0149140	0.822231	2.646379e-53	-8.615850e-47
Msa0062440	Msa0149140	0.806224	9.127197e-50	-8.615850e-47
Msa0074650	Msa0149140	0.821915	3.131527e-53	-8.615850e-47
Msa0080990	Msa0149140	0.813925	1.999664e-51	-8.615850e-47
Msa0102990	Msa0149140	0.822774	1.978966e-53	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0149140	MtrunA17_Chr1g0148801	98.030	406	8	0	1	406	1	406	0.0	835
Msa0149140	MtrunA17_Chr6g0485621	60.668	389	145	2	13	393	29	417	0.0	514
Msa0149140	MtrunA17_Chr7g0262591	58.005	381	157	2	9	388	17	395	1.37e-177	501
Msa0149140	MtrunA17_Chr1g0147221	62.078	385	142	2	9	389	35	419	2.01e-177	501
Msa0149140	MtrunA17_Chr1g0182991	57.360	394	164	3	9	398	18	411	2.33e-175	496
Msa0149140	MtrunA17_Chr1g0169111	57.216	388	160	1	7	388	93	480	3.79e-173	493
Msa0149140	MtrunA17_Chr3g0139441	59.788	378	147	3	11	383	34	411	5.14e-171	484
Msa0149140	MtrunA17_Chr7g0262471	54.575	306	136	2	84	388	213	516	6.12e-125	371
Msa0149140	MtrunA17_Chr7g0268621	44.652	374	196	5	17	384	7	375	8.88e-115	340
Msa0149140	MtrunA17_Chr8g0350371	45.213	376	195	4	17	386	7	377	9.38e-115	340

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0149140	AT5G11320.1	71.788	397	106	2	11	403	12	406	0.0	599
Msa0149140	AT4G32540.1	63.000	400	143	3	11	406	16	414	0.0	551
Msa0149140	AT4G32540.2	62.935	402	142	4	11	406	16	416	0.0	546
Msa0149140	AT5G11320.2	72.531	324	87	1	11	334	12	333	7.55e-178	499
Msa0149140	AT4G13260.1	60.256	390	150	2	2	386	14	403	4.33e-177	500
Msa0149140	AT1G04610.1	57.364	387	158	1	9	388	30	416	1.43e-174	494
Msa0149140	AT1G04180.1	57.990	388	159	3	4	388	14	400	2.36e-173	491
Msa0149140	AT5G43890.1	55.527	389	167	2	6	388	15	403	8.38e-171	484
Msa0149140	AT4G28720.1	56.072	387	163	2	9	388	18	404	1.47e-169	481
Msa0149140	AT5G25620.2	58.312	391	157	4	10	395	27	416	8.31e-169	480

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Find 64 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTCCTGTACTTGATGT+TGG	0.551364	1_4:-9699255	Msa0149140:CDS
AAACACCCTTTCCTCATGGT+TGG	0.503476	1_4:-9698425	Msa0149140:CDS
AATATCAAAGTGATCAGCAT+AGG	0.584130	1_4:+9700573	None:intergenic
AATTAAATCCGGTAACATTA+AGG	0.303146	1_4:-9699220	Msa0149140:intron
ACAAGAGCAATGTGCCTACT+TGG	0.453063	1_4:-9699000	Msa0149140:CDS
ACACAATGCTATGCCTCATT+TGG	0.405714	1_4:-9700263	Msa0149140:CDS
ACAGTGGGTTTCACAAGAAG+AGG	0.597945	1_4:-9698381	Msa0149140:CDS
ACATCAGTTCATTTCCTACA+TGG	0.512049	1_4:-9700599	Msa0149140:CDS
ACCTTTCCAACCATGAGGAA+AGG	0.554382	1_4:+9698419	None:intergenic
AGAACAGTTTGATTAAACCT+AGG	0.595686	1_4:+9700547	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGAAAAAGCAAAGTGTGTA+TGG	-	chr1_4:9698276-9698295	Msa0149140:CDS	30.0%
GGTAGTTCACAAAAATGTTT+AGG	+	chr1_4:9698458-9698477	None:intergenic	30.0%
AATATCAAAGTGATCAGCAT+AGG	+	chr1_4:9698549-9698568	None:intergenic	30.0%
ATCACTTTGATATTCATCCT+AGG	-	chr1_4:9698555-9698574	Msa0149140:intron	30.0%
AGAACAGTTTGATTAAACCT+AGG	+	chr1_4:9698575-9698594	None:intergenic	30.0%
AAGTGACCATTAAAAAGGAA+CGG	+	chr1_4:9699172-9699191	None:intergenic	30.0%
CTCTCTGCAATAAATAATCA+AGG	-	chr1_4:9699305-9699324	Msa0149140:CDS	30.0%
ATTCTTCCAAGAGATATGTT+TGG	-	chr1_4:9699678-9699697	Msa0149140:intron	30.0%
TCAACATATGGAATAGCTAT+GGG	-	chr1_4:9699705-9699724	Msa0149140:intron	30.0%
TTAGTTTCAAGCTTCTTCTT+GGG	-	chr1_4:9699774-9699793	Msa0149140:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	9698263	9700878	9698263	ID=Msa0149140;Name=Msa0149140
chr1_4	mRNA	9698263	9700878	9698263	ID=Msa0149140-mRNA-1;Parent=Msa0149140;Name=Msa0149140-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0\|0\|0\|1\|1\|1\|4\|0\|406
chr1_4	exon	9700249	9700878	9700249	ID=Msa0149140-mRNA-1:exon:4097;Parent=Msa0149140-mRNA-1
chr1_4	exon	9699221	9699469	9699221	ID=Msa0149140-mRNA-1:exon:4096;Parent=Msa0149140-mRNA-1
chr1_4	exon	9698994	9699116	9698994	ID=Msa0149140-mRNA-1:exon:4095;Parent=Msa0149140-mRNA-1
chr1_4	exon	9698263	9698481	9698263	ID=Msa0149140-mRNA-1:exon:4094;Parent=Msa0149140-mRNA-1
chr1_4	CDS	9700249	9700878	9700249	ID=Msa0149140-mRNA-1:cds;Parent=Msa0149140-mRNA-1
chr1_4	CDS	9699221	9699469	9699221	ID=Msa0149140-mRNA-1:cds;Parent=Msa0149140-mRNA-1
chr1_4	CDS	9698994	9699116	9698994	ID=Msa0149140-mRNA-1:cds;Parent=Msa0149140-mRNA-1
chr1_4	CDS	9698263	9698481	9698263	ID=Msa0149140-mRNA-1:cds;Parent=Msa0149140-mRNA-1

Gene Sequence

>Msa0149140

ATGGATCCTTTCAAAGAAAAAGCAAAGTGTGTATGGATTCATGGACCTATCATAGTAGGTGCTGGTCCTTCAGGTATAGCAGTAGCTGCTTGTTTATCAGAACAAGGAGTACCGAGTCTCATTCTTGAGAGAAGTGATTGCATAGCTTCACTATGGCAAAATAGAACCTATGACCGTTTAAAACTTCATTTACCTAAACATTTTTGTGAACTACCTATGATGAGTTTTCCTCAAACATTTCCTAAGTATCCTACAAAACATCAGTTCATTTCCTACATGGAATCCTATGCTGATCACTTTGATATTCATCCTAGGTTTAATCAAACTGTTCTTAGTGCTGAGTTTGATTCAAGTTCTCAAATTTGGATGGTTAGAACTAAAGAAGCTGATTTTCAGTATTTTTCACCTTGGCTTATTGTTGCTACTGGTGAAAATGCTGAACCTGTTTTTCCAACAATTCATGGTATGGAACATTTTCATGGTCCTGTTGCTCATACAAGTGATTATAAATCTGGTTCTGAGTACAAAAATAAAAAGGTTTTGGTCATTGGTTGTGGGAATTCTGGAATGGAAGTTAGTTTGGATCTTTGTAGACACAATGCTATGCCTCATTTGGTTGCACGAAACTCAGTACATATTCTTCCAAGAGATATGTTTGGTTTCTCAACATATGGAATAGCTATGGGATTATATAAATGGCTTCCATTAAAATTAGTAGACAAGTTTCTCTTGTTAGTTTCAAGCTTCTTCTTGGGAAACACCAATCACTATGGTATCAAGAGGCCTAAAACTGGTCCAATAGAACTCAAACTTGCAACAGGAAAAACTCCTGTACTTGATGTTGGTCAAATAGCACAAATTAAATCCGGTAACATTAAGGTGATGGAAGGTGTGAAAGAGATAACAAGAAATGGTGCAAAATTTTTGGATGGACAAGAAAAGGAATTTGATGCAATAATCTTAGCAACAGGGTACAAGAGCAATGTGCCTACTTGGCTCAAGGGTAATGATTTTTTCACTAAAGATGGAATGCCAAAAACACCCTTTCCTCATGGTTGGAAAGGTGAGCAAGGATTGTATACAGTGGGTTTCACAAGAAGAGGTCTACATGGAACATACTTTGATGCCATCAAAATAGCTGAAGATATTACTAATCAATGGAAAGCATTAAAGAACAAGAGTTGTTGTGATTCACATATCATACTACTCAATAATTCATAA

Protein sequence

>Msa0149140

MDPFKEKAKCVWIHGPIIVGAGPSGIAVAACLSEQGVPSLILERSDCIASLWQNRTYDRLKLHLPKHFCELPMMSFPQTFPKYPTKHQFISYMESYADHFDIHPRFNQTVLSAEFDSSSQIWMVRTKEADFQYFSPWLIVATGENAEPVFPTIHGMEHFHGPVAHTSDYKSGSEYKNKKVLVIGCGNSGMEVSLDLCRHNAMPHLVARNSVHILPRDMFGFSTYGIAMGLYKWLPLKLVDKFLLLVSSFFLGNTNHYGIKRPKTGPIELKLATGKTPVLDVGQIAQIKSGNIKVMEGVKEITRNGAKFLDGQEKEFDAIILATGYKSNVPTWLKGNDFFTKDGMPKTPFPHGWKGEQGLYTVGFTRRGLHGTYFDAIKIAEDITNQWKALKNKSCCDSHIILLNNS*