AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0423100


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0423100 MtrunA17_Chr3g0123851 60.656 122 8 2 1 83 1 121 3.68e-39 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 34 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATAACCTCATCTTGTTAA+TGG 0.240049 3_2:+72779930 Msa0423100:CDS
TGTAGTGGAGGCTGAGATAG+AGG 0.383685 3_2:-72779603 None:intergenic
ATCCGTTATTATTATTACCT+TGG 0.417680 3_2:-72780487 None:intergenic
GGTTGATAGAAGCAAGAAAC+TGG 0.429204 3_2:-72779985 None:intergenic
CTCCAAGGTAATAATAATAA+CGG 0.464837 3_2:+72780485 Msa0423100:CDS
AAGAAACTGGACACAGTAGA+AGG 0.491502 3_2:-72779972 None:intergenic
CAATGGCATTTCCAACTCAA+TGG 0.495776 3_2:-72780524 None:intergenic
AAAGAAATTACCACCTTCCA+TGG 0.501489 3_2:-72780462 None:intergenic
CACTGCCTCAACTTCATCCA+TGG 0.509432 3_2:+72780445 Msa0423100:CDS
AGGGAAAAGCCTACCCTTGG+AGG 0.512819 3_2:-72780021 None:intergenic
ATTTCTAAGGGTTGAAGAAG+TGG 0.515036 3_2:-72780553 None:intergenic
GGAGAGGGATAGTGTAGTGG+AGG 0.517083 3_2:-72779615 None:intergenic
CAAATTACAAGCCATTGAGT+TGG 0.518087 3_2:+72780513 Msa0423100:CDS
GGTAACAATTGTGTAGAGAA+AGG 0.520211 3_2:-72779636 None:intergenic
AAAGGAGAGGGATAGTGTAG+TGG 0.532448 3_2:-72779618 None:intergenic
GGTGGTAATTTCTTTCTCCA+AGG 0.542564 3_2:+72780470 Msa0423100:CDS
CACATTCTGCAATTCCTCCA+AGG 0.543179 3_2:+72780007 Msa0423100:CDS
AGATAGAGGGTGCAGAGTGT+TGG 0.551815 3_2:-72779589 None:intergenic
GAAGTTGAGGCAGTGTGACC+TGG 0.560053 3_2:-72780437 None:intergenic
AATTGTGTAGAGAAAGGAGA+GGG 0.568206 3_2:-72779630 None:intergenic
ACCTTCCATGGATGAAGTTG+AGG 0.576895 3_2:-72780450 None:intergenic
GCCTCAACTTCATCCATGGA+AGG 0.583985 3_2:+72780449 Msa0423100:CDS
ATATGAATTAAATGAAGCCA+TGG 0.598193 3_2:-72779911 None:intergenic
GTAGTGGAGGCTGAGATAGA+GGG 0.606138 3_2:-72779602 None:intergenic
TGATGACCATTAACAAGATG+AGG 0.607087 3_2:-72779936 None:intergenic
TTGAGTTGGAAATGCCATTG+TGG 0.619909 3_2:+72780527 Msa0423100:CDS
TGAAGAAGTGGCCACCACAA+TGG 0.621299 3_2:-72780541 None:intergenic
ACATTCTGCAATTCCTCCAA+GGG 0.630675 3_2:+72780008 Msa0423100:CDS
ACATGATAGATAGATATCCA+TGG 0.649649 3_2:+72779894 Msa0423100:exon
AGTGTTGGCAGAAAAGTGAA+AGG 0.656008 3_2:-72779574 None:intergenic
TCAACTTCATCCATGGAAGG+TGG 0.660618 3_2:+72780452 Msa0423100:CDS
CTTGGAGGAATTGCAGAATG+TGG 0.672231 3_2:-72780006 None:intergenic
AGTTGGAAATGCCATTGTGG+TGG 0.687706 3_2:+72780530 Msa0423100:CDS
CAATTGTGTAGAGAAAGGAG+AGG 0.717317 3_2:-72779631 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTTTGAATTCACTATAAAAT+TGG - chr3_2:72780215-72780234 None:intergenic 20.0%
!! AGTTAACAAACTTAATTAAC+TGG - chr3_2:72780290-72780309 None:intergenic 20.0%
!! GTTTGAATTCACTATAAAAT+TGG - chr3_2:72780215-72780234 None:intergenic 20.0%
!! AGTTAACAAACTTAATTAAC+TGG - chr3_2:72780290-72780309 None:intergenic 20.0%
!!! GTTTTTTTCTAGTTATGTGT+TGG - chr3_2:72779664-72779683 None:intergenic 25.0%
!! AATGACAAACTTTTCATTCT+TGG + chr3_2:72779708-72779727 Msa0423100:five_prime_UTR 25.0%
!!! TTATTTTGTCACATTACAAC+TGG + chr3_2:72779779-72779798 Msa0423100:five_prime_UTR 25.0%
! ATATGAATTAAATGAAGCCA+TGG - chr3_2:72779914-72779933 None:intergenic 25.0%
! ATATAACCTCATCTTGTTAA+TGG + chr3_2:72779930-72779949 Msa0423100:CDS 25.0%
!!! TAGTTGGTTTTAAAAGGAAT+CGG + chr3_2:72780091-72780110 Msa0423100:intron 25.0%
!! ACAGTATATTACTGTGATTT+TGG + chr3_2:72780149-72780168 Msa0423100:intron 25.0%
! AATTCACTATAAAATTGGCT+CGG - chr3_2:72780210-72780229 None:intergenic 25.0%
! TATTATTTGAGGAGAAAAAC+AGG + chr3_2:72780324-72780343 Msa0423100:intron 25.0%
! CTCCAAGGTAATAATAATAA+CGG + chr3_2:72780485-72780504 Msa0423100:CDS 25.0%
! ATCCGTTATTATTATTACCT+TGG - chr3_2:72780490-72780509 None:intergenic 25.0%
!!! GTTTTTTTCTAGTTATGTGT+TGG - chr3_2:72779664-72779683 None:intergenic 25.0%
!! AATGACAAACTTTTCATTCT+TGG + chr3_2:72779708-72779727 Msa0423100:five_prime_UTR 25.0%
!!! TTATTTTGTCACATTACAAC+TGG + chr3_2:72779779-72779798 Msa0423100:five_prime_UTR 25.0%
! ATATGAATTAAATGAAGCCA+TGG - chr3_2:72779914-72779933 None:intergenic 25.0%
! ATATAACCTCATCTTGTTAA+TGG + chr3_2:72779930-72779949 Msa0423100:CDS 25.0%
!!! TAGTTGGTTTTAAAAGGAAT+CGG + chr3_2:72780091-72780110 Msa0423100:intron 25.0%
!! ACAGTATATTACTGTGATTT+TGG + chr3_2:72780149-72780168 Msa0423100:intron 25.0%
! AATTCACTATAAAATTGGCT+CGG - chr3_2:72780210-72780229 None:intergenic 25.0%
! TATTATTTGAGGAGAAAAAC+AGG + chr3_2:72780324-72780343 Msa0423100:intron 25.0%
! CTCCAAGGTAATAATAATAA+CGG + chr3_2:72780485-72780504 Msa0423100:CDS 25.0%
! ATCCGTTATTATTATTACCT+TGG - chr3_2:72780490-72780509 None:intergenic 25.0%
!!! ATCTAGTTTTGAGCAATGAA+TGG - chr3_2:72779686-72779705 None:intergenic 30.0%
ACATGATAGATAGATATCCA+TGG + chr3_2:72779894-72779913 Msa0423100:exon 30.0%
TTATGTGTATGTTGCATAGT+TGG + chr3_2:72780075-72780094 Msa0423100:intron 30.0%
!!! GTTGCATAGTTGGTTTTAAA+AGG + chr3_2:72780085-72780104 Msa0423100:intron 30.0%
ACAGTAATATACTGTGATTC+TGG - chr3_2:72780144-72780163 None:intergenic 30.0%
! ATCCTCATTTTGTTGAAACT+TGG - chr3_2:72780243-72780262 None:intergenic 30.0%
ACTTAATTAACTGGTGAGTT+TGG - chr3_2:72780281-72780300 None:intergenic 30.0%
!! AGTTTGTTAACTATGTGTGT+AGG + chr3_2:72780298-72780317 Msa0423100:intron 30.0%
! GTTTGTTAACTATGTGTGTA+GGG + chr3_2:72780299-72780318 Msa0423100:intron 30.0%
TGTGTAGGGTTTATTATTTG+AGG + chr3_2:72780313-72780332 Msa0423100:intron 30.0%
!! TTACATTTGTTGTGACATGT+AGG - chr3_2:72780365-72780384 None:intergenic 30.0%
!!! ATCTAGTTTTGAGCAATGAA+TGG - chr3_2:72779686-72779705 None:intergenic 30.0%
ACATGATAGATAGATATCCA+TGG + chr3_2:72779894-72779913 Msa0423100:exon 30.0%
TTATGTGTATGTTGCATAGT+TGG + chr3_2:72780075-72780094 Msa0423100:intron 30.0%
!!! GTTGCATAGTTGGTTTTAAA+AGG + chr3_2:72780085-72780104 Msa0423100:intron 30.0%
ACAGTAATATACTGTGATTC+TGG - chr3_2:72780144-72780163 None:intergenic 30.0%
! ATCCTCATTTTGTTGAAACT+TGG - chr3_2:72780243-72780262 None:intergenic 30.0%
ACTTAATTAACTGGTGAGTT+TGG - chr3_2:72780281-72780300 None:intergenic 30.0%
!! AGTTTGTTAACTATGTGTGT+AGG + chr3_2:72780298-72780317 Msa0423100:intron 30.0%
! GTTTGTTAACTATGTGTGTA+GGG + chr3_2:72780299-72780318 Msa0423100:intron 30.0%
TGTGTAGGGTTTATTATTTG+AGG + chr3_2:72780313-72780332 Msa0423100:intron 30.0%
!! TTACATTTGTTGTGACATGT+AGG - chr3_2:72780365-72780384 None:intergenic 30.0%
AATTGTGTAGAGAAAGGAGA+GGG - chr3_2:72779633-72779652 None:intergenic 35.0%
GGTAACAATTGTGTAGAGAA+AGG - chr3_2:72779639-72779658 None:intergenic 35.0%
!!! TTTTCTAGTTATGTGTTGGC+AGG - chr3_2:72779660-72779679 None:intergenic 35.0%
TGATGACCATTAACAAGATG+AGG - chr3_2:72779939-72779958 None:intergenic 35.0%
AGTGAATGAGGGAGAATAAA+GGG - chr3_2:72780043-72780062 None:intergenic 35.0%
GCAACATACACATAAAGCAA+AGG - chr3_2:72780070-72780089 None:intergenic 35.0%
CACCAAGTTTCAACAAAATG+AGG + chr3_2:72780238-72780257 Msa0423100:intron 35.0%
TTTGAGGAGAAAAACAGGTT+AGG + chr3_2:72780329-72780348 Msa0423100:intron 35.0%
AAAGAAATTACCACCTTCCA+TGG - chr3_2:72780465-72780484 None:intergenic 35.0%
CAAATTACAAGCCATTGAGT+TGG + chr3_2:72780513-72780532 Msa0423100:CDS 35.0%
AATTGTGTAGAGAAAGGAGA+GGG - chr3_2:72779633-72779652 None:intergenic 35.0%
GGTAACAATTGTGTAGAGAA+AGG - chr3_2:72779639-72779658 None:intergenic 35.0%
!!! TTTTCTAGTTATGTGTTGGC+AGG - chr3_2:72779660-72779679 None:intergenic 35.0%
TGATGACCATTAACAAGATG+AGG - chr3_2:72779939-72779958 None:intergenic 35.0%
AGTGAATGAGGGAGAATAAA+GGG - chr3_2:72780043-72780062 None:intergenic 35.0%
GCAACATACACATAAAGCAA+AGG - chr3_2:72780070-72780089 None:intergenic 35.0%
CACCAAGTTTCAACAAAATG+AGG + chr3_2:72780238-72780257 Msa0423100:intron 35.0%
TTTGAGGAGAAAAACAGGTT+AGG + chr3_2:72780329-72780348 Msa0423100:intron 35.0%
AAAGAAATTACCACCTTCCA+TGG - chr3_2:72780465-72780484 None:intergenic 35.0%
CAAATTACAAGCCATTGAGT+TGG + chr3_2:72780513-72780532 Msa0423100:CDS 35.0%
CAATTGTGTAGAGAAAGGAG+AGG - chr3_2:72779634-72779653 None:intergenic 40.0%
AAGAAACTGGACACAGTAGA+AGG - chr3_2:72779975-72779994 None:intergenic 40.0%
!! GGTTGATAGAAGCAAGAAAC+TGG - chr3_2:72779988-72780007 None:intergenic 40.0%
ACATTCTGCAATTCCTCCAA+GGG + chr3_2:72780008-72780027 Msa0423100:CDS 40.0%
GAGTGAATGAGGGAGAATAA+AGG - chr3_2:72780044-72780063 None:intergenic 40.0%
GCAAAGGAAAGAGTGAATGA+GGG - chr3_2:72780054-72780073 None:intergenic 40.0%
AGCAAAGGAAAGAGTGAATG+AGG - chr3_2:72780055-72780074 None:intergenic 40.0%
TGGTGAGTTTGGCAAAATCA+AGG - chr3_2:72780270-72780289 None:intergenic 40.0%
!! CATTTGTTGTGACATGTAGG+TGG - chr3_2:72780362-72780381 None:intergenic 40.0%
TAACCAATGTCATCCATGCA+TGG + chr3_2:72780382-72780401 Msa0423100:intron 40.0%
GGTGGTAATTTCTTTCTCCA+AGG + chr3_2:72780470-72780489 Msa0423100:CDS 40.0%
CAATGGCATTTCCAACTCAA+TGG - chr3_2:72780527-72780546 None:intergenic 40.0%
!! TTGAGTTGGAAATGCCATTG+TGG + chr3_2:72780527-72780546 Msa0423100:CDS 40.0%
CAATTGTGTAGAGAAAGGAG+AGG - chr3_2:72779634-72779653 None:intergenic 40.0%
AAGAAACTGGACACAGTAGA+AGG - chr3_2:72779975-72779994 None:intergenic 40.0%
!! GGTTGATAGAAGCAAGAAAC+TGG - chr3_2:72779988-72780007 None:intergenic 40.0%
ACATTCTGCAATTCCTCCAA+GGG + chr3_2:72780008-72780027 Msa0423100:CDS 40.0%
GAGTGAATGAGGGAGAATAA+AGG - chr3_2:72780044-72780063 None:intergenic 40.0%
GCAAAGGAAAGAGTGAATGA+GGG - chr3_2:72780054-72780073 None:intergenic 40.0%
AGCAAAGGAAAGAGTGAATG+AGG - chr3_2:72780055-72780074 None:intergenic 40.0%
TGGTGAGTTTGGCAAAATCA+AGG - chr3_2:72780270-72780289 None:intergenic 40.0%
!! CATTTGTTGTGACATGTAGG+TGG - chr3_2:72780362-72780381 None:intergenic 40.0%
TAACCAATGTCATCCATGCA+TGG + chr3_2:72780382-72780401 Msa0423100:intron 40.0%
GGTGGTAATTTCTTTCTCCA+AGG + chr3_2:72780470-72780489 Msa0423100:CDS 40.0%
CAATGGCATTTCCAACTCAA+TGG - chr3_2:72780527-72780546 None:intergenic 40.0%
!! TTGAGTTGGAAATGCCATTG+TGG + chr3_2:72780527-72780546 Msa0423100:CDS 40.0%
AAAGGAGAGGGATAGTGTAG+TGG - chr3_2:72779621-72779640 None:intergenic 45.0%
CTTGGAGGAATTGCAGAATG+TGG - chr3_2:72780009-72780028 None:intergenic 45.0%
CACATTCTGCAATTCCTCCA+AGG + chr3_2:72780007-72780026 Msa0423100:CDS 45.0%
TAAAGGGAAAAGCCTACCCT+TGG - chr3_2:72780027-72780046 None:intergenic 45.0%
GGAATCGGTGCATGTTTGAA+TGG + chr3_2:72780106-72780125 Msa0423100:intron 45.0%
TCGGTGCATGTTTGAATGGA+CGG + chr3_2:72780110-72780129 Msa0423100:intron 45.0%
GCTCGGAGCTTCAACAAAAT+CGG - chr3_2:72780193-72780212 None:intergenic 45.0%
ACCTTCCATGGATGAAGTTG+AGG - chr3_2:72780453-72780472 None:intergenic 45.0%
TCAACTTCATCCATGGAAGG+TGG + chr3_2:72780452-72780471 Msa0423100:CDS 45.0%
AGTTGGAAATGCCATTGTGG+TGG + chr3_2:72780530-72780549 Msa0423100:CDS 45.0%
AAAGGAGAGGGATAGTGTAG+TGG - chr3_2:72779621-72779640 None:intergenic 45.0%
CTTGGAGGAATTGCAGAATG+TGG - chr3_2:72780009-72780028 None:intergenic 45.0%
CACATTCTGCAATTCCTCCA+AGG + chr3_2:72780007-72780026 Msa0423100:CDS 45.0%
TAAAGGGAAAAGCCTACCCT+TGG - chr3_2:72780027-72780046 None:intergenic 45.0%
GGAATCGGTGCATGTTTGAA+TGG + chr3_2:72780106-72780125 Msa0423100:intron 45.0%
TCGGTGCATGTTTGAATGGA+CGG + chr3_2:72780110-72780129 Msa0423100:intron 45.0%
GCTCGGAGCTTCAACAAAAT+CGG - chr3_2:72780193-72780212 None:intergenic 45.0%
ACCTTCCATGGATGAAGTTG+AGG - chr3_2:72780453-72780472 None:intergenic 45.0%
TCAACTTCATCCATGGAAGG+TGG + chr3_2:72780452-72780471 Msa0423100:CDS 45.0%
AGTTGGAAATGCCATTGTGG+TGG + chr3_2:72780530-72780549 Msa0423100:CDS 45.0%
! AGATAGAGGGTGCAGAGTGT+TGG - chr3_2:72779592-72779611 None:intergenic 50.0%
! GTAGTGGAGGCTGAGATAGA+GGG - chr3_2:72779605-72779624 None:intergenic 50.0%
! TGTAGTGGAGGCTGAGATAG+AGG - chr3_2:72779606-72779625 None:intergenic 50.0%
TCTGCAATTCCTCCAAGGGT+AGG + chr3_2:72780012-72780031 Msa0423100:intron 50.0%
CTGCCATGCATGGATGACAT+TGG - chr3_2:72780388-72780407 None:intergenic 50.0%
GGCTGAACATGTTCATGGCT+TGG - chr3_2:72780419-72780438 None:intergenic 50.0%
AGCCATGAACATGTTCAGCC+AGG + chr3_2:72780419-72780438 Msa0423100:intron 50.0%
GACCTGGCTGAACATGTTCA+TGG - chr3_2:72780424-72780443 None:intergenic 50.0%
CACTGCCTCAACTTCATCCA+TGG + chr3_2:72780445-72780464 Msa0423100:CDS 50.0%
GCCTCAACTTCATCCATGGA+AGG + chr3_2:72780449-72780468 Msa0423100:CDS 50.0%
TGAAGAAGTGGCCACCACAA+TGG - chr3_2:72780544-72780563 None:intergenic 50.0%
! AGATAGAGGGTGCAGAGTGT+TGG - chr3_2:72779592-72779611 None:intergenic 50.0%
! GTAGTGGAGGCTGAGATAGA+GGG - chr3_2:72779605-72779624 None:intergenic 50.0%
! TGTAGTGGAGGCTGAGATAG+AGG - chr3_2:72779606-72779625 None:intergenic 50.0%
TCTGCAATTCCTCCAAGGGT+AGG + chr3_2:72780012-72780031 Msa0423100:intron 50.0%
CTGCCATGCATGGATGACAT+TGG - chr3_2:72780388-72780407 None:intergenic 50.0%
GGCTGAACATGTTCATGGCT+TGG - chr3_2:72780419-72780438 None:intergenic 50.0%
AGCCATGAACATGTTCAGCC+AGG + chr3_2:72780419-72780438 Msa0423100:intron 50.0%
GACCTGGCTGAACATGTTCA+TGG - chr3_2:72780424-72780443 None:intergenic 50.0%
CACTGCCTCAACTTCATCCA+TGG + chr3_2:72780445-72780464 Msa0423100:CDS 50.0%
GCCTCAACTTCATCCATGGA+AGG + chr3_2:72780449-72780468 Msa0423100:CDS 50.0%
TGAAGAAGTGGCCACCACAA+TGG - chr3_2:72780544-72780563 None:intergenic 50.0%
GGAGAGGGATAGTGTAGTGG+AGG - chr3_2:72779618-72779637 None:intergenic 55.0%
AGGGAAAAGCCTACCCTTGG+AGG - chr3_2:72780024-72780043 None:intergenic 55.0%
GGAACTTGCACTGCCATGCA+TGG - chr3_2:72780398-72780417 None:intergenic 55.0%
GAAGTTGAGGCAGTGTGACC+TGG - chr3_2:72780440-72780459 None:intergenic 55.0%
GGAGAGGGATAGTGTAGTGG+AGG - chr3_2:72779618-72779637 None:intergenic 55.0%
AGGGAAAAGCCTACCCTTGG+AGG - chr3_2:72780024-72780043 None:intergenic 55.0%
GGAACTTGCACTGCCATGCA+TGG - chr3_2:72780398-72780417 None:intergenic 55.0%
GAAGTTGAGGCAGTGTGACC+TGG - chr3_2:72780440-72780459 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 72779576 72780571 72779576 ID=Msa0423100;Name=Msa0423100
chr3_2 mRNA 72779576 72780571 72779576 ID=Msa0423100-mRNA-1;Parent=Msa0423100;Name=Msa0423100-mRNA-1;_AED=0.16;_eAED=0.17;_QI=337|0|0.5|1|0|0|2|0|83
chr3_2 exon 72779576 72780029 72779576 ID=Msa0423100-mRNA-1:exon:18030;Parent=Msa0423100-mRNA-1
chr3_2 exon 72780437 72780571 72780437 ID=Msa0423100-mRNA-1:exon:18031;Parent=Msa0423100-mRNA-1
chr3_2 five_prime_UTR 72779576 72779912 72779576 ID=Msa0423100-mRNA-1:five_prime_utr;Parent=Msa0423100-mRNA-1
chr3_2 CDS 72779913 72780029 72779913 ID=Msa0423100-mRNA-1:cds;Parent=Msa0423100-mRNA-1
chr3_2 CDS 72780437 72780571 72780437 ID=Msa0423100-mRNA-1:cds;Parent=Msa0423100-mRNA-1
Gene Sequence

>Msa0423100

ATGGCTTCATTTAATTCATATAACCTCATCTTGTTAATGGTCATCATATTTATACTTCACCTTCTACTGTGTCCAGTTTCTTGCTTCTATCAACCACATTCTGCAATTCCTCCAAGGCCAGGTCACACTGCCTCAACTTCATCCATGGAAGGTGGTAATTTCTTTCTCCAAGGTAATAATAATAACGGATACTCAAATTACAAGCCATTGAGTTGGAAATGCCATTGTGGTGGCCACTTCTTCAACCCTTAG

Protein sequence

>Msa0423100

MASFNSYNLILLMVIIFILHLLLCPVSCFYQPHSAIPPRPGHTASTSSMEGGNFFLQGNNNNGYSNYKPLSWKCHCGGHFFNP*