AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0911990


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0911990 MtrunA17_Chr6g0464801 44.828 87 23 2 53 135 7 72 2.82e-13 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 24 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAACATTTGCTTCTTCATT+TGG 0.284164 6_2:+19529695 None:intergenic
TTATCAGCACTTTGATAGTT+AGG 0.296935 6_2:-19528727 Msa0911990:CDS
GTACTAAGATCTGATCTTCT+TGG 0.327969 6_2:+19528659 None:intergenic
GTACTAAGATCTGATCTTCT+TGG 0.327969 6_2:+19528695 None:intergenic
GAAACTTTCTTCAAATGTCT+AGG 0.440784 6_2:-19529611 Msa0911990:CDS
CTTAGTATTGGAAGAGGTCC+AGG 0.443199 6_2:-19528581 Msa0911990:CDS
AAACTTTCTTCAAATGTCTA+GGG 0.469767 6_2:-19529610 Msa0911990:CDS
GGAAGATCAGATCTTAGTAC+TGG 0.477627 6_2:-19528629 Msa0911990:CDS
GATGCAGAAGAGAATCTTGA+TGG 0.479422 6_2:-19529578 Msa0911990:CDS
AGAAGATCAGATCTTAGTAT+TGG 0.495981 6_2:-19528593 Msa0911990:CDS
ACAAATGCTGAATTAATCCC+TGG 0.505603 6_2:+19528563 None:intergenic
TCAGATCTTAGTACTGGAAG+AGG 0.510467 6_2:-19528650 Msa0911990:CDS
AGAAGATCAGATCTTAGTAC+TGG 0.511957 6_2:-19528656 Msa0911990:CDS
AGAAGATCAGATCTTAGTAC+TGG 0.511957 6_2:-19528692 Msa0911990:CDS
AAAGAATGGTCAACGAGTAA+TGG 0.539417 6_2:-19529530 Msa0911990:CDS
TTTGGAGTAAAGAACATGGA+TGG 0.540921 6_2:+19529642 None:intergenic
TCAGATCTTAGTACTGGAAG+AGG 0.548826 6_2:-19528623 Msa0911990:CDS
TCAGATCTTAGTACTGGAAG+AGG 0.548826 6_2:-19528686 Msa0911990:CDS
ACTTTGATAGTTAGGAGAGA+TGG 0.558375 6_2:-19528719 Msa0911990:CDS
TCAGATCTTAGTATTGGAAG+AGG 0.585484 6_2:-19528587 Msa0911990:CDS
AAGCAAATGTTACAAATGAG+AGG 0.594342 6_2:-19529685 Msa0911990:CDS
GATCTTAGTACTGGAAGAGG+TGG 0.595804 6_2:-19528683 Msa0911990:CDS
GATCTTAGTACTGGAAGAGG+TGG 0.621063 6_2:-19528620 Msa0911990:CDS
TTAGTATTGGAAGAGGTCCA+GGG 0.652914 6_2:-19528580 Msa0911990:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTATTTATTTATTTTTTT+GGG + chr6_2:19529198-19529217 None:intergenic 0.0%
!!! TAATTATTTATTTATTTTTT+TGG + chr6_2:19529199-19529218 None:intergenic 0.0%
!!! AATTATTTATTTATTTTTTT+GGG + chr6_2:19529198-19529217 None:intergenic 0.0%
!!! TAATTATTTATTTATTTTTT+TGG + chr6_2:19529199-19529218 None:intergenic 0.0%
!! AGTAAAAAAAATGATTAATT+TGG - chr6_2:19528909-19528928 Msa0911990:intron 10.0%
!! TATAAATGTGTTAAAAATAT+GGG + chr6_2:19529159-19529178 None:intergenic 10.0%
!! TTATAAATGTGTTAAAAATA+TGG + chr6_2:19529160-19529179 None:intergenic 10.0%
!! AGTAAAAAAAATGATTAATT+TGG - chr6_2:19528909-19528928 Msa0911990:intron 10.0%
!! TATAAATGTGTTAAAAATAT+GGG + chr6_2:19529159-19529178 None:intergenic 10.0%
!! TTATAAATGTGTTAAAAATA+TGG + chr6_2:19529160-19529179 None:intergenic 10.0%
!!! AATTAAGAGTTGATATTTTT+AGG - chr6_2:19528954-19528973 Msa0911990:intron 15.0%
!!! ATTAAGAGTTGATATTTTTA+GGG - chr6_2:19528955-19528974 Msa0911990:intron 15.0%
!!! AATTAAGAGTTGATATTTTT+AGG - chr6_2:19528954-19528973 Msa0911990:intron 15.0%
!!! ATTAAGAGTTGATATTTTTA+GGG - chr6_2:19528955-19528974 Msa0911990:intron 15.0%
!!! AGAAAGTTTCAAGAAATTTT+TGG + chr6_2:19528648-19528667 None:intergenic 20.0%
!! AAGAAAAAGAGAATACAAAT+CGG + chr6_2:19529514-19529533 None:intergenic 20.0%
!!! AGAAAGTTTCAAGAAATTTT+TGG + chr6_2:19528648-19528667 None:intergenic 20.0%
!! AAGAAAAAGAGAATACAAAT+CGG + chr6_2:19529514-19529533 None:intergenic 20.0%
!!! AATTTTTGGAGTAAAGAACA+TGG + chr6_2:19528634-19528653 None:intergenic 25.0%
! AAACTTTCTTCAAATGTCTA+GGG - chr6_2:19528659-19528678 Msa0911990:CDS 25.0%
!!! AATGTTAACTTGTGTCATTT+TGG - chr6_2:19528979-19528998 Msa0911990:intron 25.0%
! ACAACTAAATGTTAGTATGT+TGG - chr6_2:19529234-19529253 Msa0911990:intron 25.0%
!! TTCAGATTAAGTTGTTAATG+AGG - chr6_2:19529292-19529311 Msa0911990:intron 25.0%
!!! AGTTGTTAATGAGGTTTTTT+TGG - chr6_2:19529301-19529320 Msa0911990:intron 25.0%
! AGGTTAAACACAAATCATTT+AGG - chr6_2:19529428-19529447 Msa0911990:intron 25.0%
!! CATTTAGGAAAATCGAATTT+TGG - chr6_2:19529443-19529462 Msa0911990:intron 25.0%
!! ATATTCTAGAGAAAGATTGT+TGG + chr6_2:19529471-19529490 None:intergenic 25.0%
!!! AATTTTTGGAGTAAAGAACA+TGG + chr6_2:19528634-19528653 None:intergenic 25.0%
! AAACTTTCTTCAAATGTCTA+GGG - chr6_2:19528659-19528678 Msa0911990:CDS 25.0%
!!! AATGTTAACTTGTGTCATTT+TGG - chr6_2:19528979-19528998 Msa0911990:intron 25.0%
! ACAACTAAATGTTAGTATGT+TGG - chr6_2:19529234-19529253 Msa0911990:intron 25.0%
!! TTCAGATTAAGTTGTTAATG+AGG - chr6_2:19529292-19529311 Msa0911990:intron 25.0%
!!! AGTTGTTAATGAGGTTTTTT+TGG - chr6_2:19529301-19529320 Msa0911990:intron 25.0%
! AGGTTAAACACAAATCATTT+AGG - chr6_2:19529428-19529447 Msa0911990:intron 25.0%
!! CATTTAGGAAAATCGAATTT+TGG - chr6_2:19529443-19529462 Msa0911990:intron 25.0%
!! ATATTCTAGAGAAAGATTGT+TGG + chr6_2:19529471-19529490 None:intergenic 25.0%
GTAACATTTGCTTCTTCATT+TGG + chr6_2:19528577-19528596 None:intergenic 30.0%
AAGCAAATGTTACAAATGAG+AGG - chr6_2:19528584-19528603 Msa0911990:CDS 30.0%
TGGATGGTAAAGATAAAAAG+TGG + chr6_2:19528614-19528633 None:intergenic 30.0%
GAAACTTTCTTCAAATGTCT+AGG - chr6_2:19528658-19528677 Msa0911990:CDS 30.0%
TTTCTTAACTTATGCCCTAA+GGG - chr6_2:19529118-19529137 Msa0911990:intron 30.0%
! TTAGTATGTTGGTGAATATG+TGG - chr6_2:19529245-19529264 Msa0911990:intron 30.0%
ACAACTTAATCTGAATGACT+TGG + chr6_2:19529287-19529306 None:intergenic 30.0%
! CTGTATCGTTCCAATTTTAT+CGG - chr6_2:19529378-19529397 Msa0911990:intron 30.0%
! TTATCAGCACTTTGATAGTT+AGG - chr6_2:19529542-19529561 Msa0911990:CDS 30.0%
AGAAGATCAGATCTTAGTAT+TGG - chr6_2:19529676-19529695 Msa0911990:CDS 30.0%
GTAACATTTGCTTCTTCATT+TGG + chr6_2:19528577-19528596 None:intergenic 30.0%
AAGCAAATGTTACAAATGAG+AGG - chr6_2:19528584-19528603 Msa0911990:CDS 30.0%
TGGATGGTAAAGATAAAAAG+TGG + chr6_2:19528614-19528633 None:intergenic 30.0%
GAAACTTTCTTCAAATGTCT+AGG - chr6_2:19528658-19528677 Msa0911990:CDS 30.0%
TTTCTTAACTTATGCCCTAA+GGG - chr6_2:19529118-19529137 Msa0911990:intron 30.0%
! TTAGTATGTTGGTGAATATG+TGG - chr6_2:19529245-19529264 Msa0911990:intron 30.0%
ACAACTTAATCTGAATGACT+TGG + chr6_2:19529287-19529306 None:intergenic 30.0%
! CTGTATCGTTCCAATTTTAT+CGG - chr6_2:19529378-19529397 Msa0911990:intron 30.0%
! TTATCAGCACTTTGATAGTT+AGG - chr6_2:19529542-19529561 Msa0911990:CDS 30.0%
AGAAGATCAGATCTTAGTAT+TGG - chr6_2:19529676-19529695 Msa0911990:CDS 30.0%
! TTTGGAGTAAAGAACATGGA+TGG + chr6_2:19528630-19528649 None:intergenic 35.0%
CATGTGCAGAACAAAAAGAA+TGG - chr6_2:19528725-19528744 Msa0911990:CDS 35.0%
AAAGAATGGTCAACGAGTAA+TGG - chr6_2:19528739-19528758 Msa0911990:intron 35.0%
!!! AACTTGTGTCATTTTGGTAC+AGG - chr6_2:19528985-19529004 Msa0911990:intron 35.0%
GTTTCTTAACTTATGCCCTA+AGG - chr6_2:19529117-19529136 Msa0911990:intron 35.0%
!! TATGTTAACTTGTGCCCTTA+GGG + chr6_2:19529135-19529154 None:intergenic 35.0%
!! TTATGTTAACTTGTGCCCTT+AGG + chr6_2:19529136-19529155 None:intergenic 35.0%
!! CTGAATGACTTGGTTCTATA+AGG + chr6_2:19529277-19529296 None:intergenic 35.0%
TCATTAACAACTGTCTCTTC+GGG + chr6_2:19529409-19529428 None:intergenic 35.0%
CTCATTAACAACTGTCTCTT+CGG + chr6_2:19529410-19529429 None:intergenic 35.0%
! ACTTTGATAGTTAGGAGAGA+TGG - chr6_2:19529550-19529569 Msa0911990:CDS 35.0%
GTACTAAGATCTGATCTTCT+TGG + chr6_2:19529577-19529596 None:intergenic 35.0%
AGAAGATCAGATCTTAGTAC+TGG - chr6_2:19529577-19529596 Msa0911990:CDS 35.0%
GTACTAAGATCTGATCTTCT+TGG + chr6_2:19529577-19529596 None:intergenic 35.0%
AGAAGATCAGATCTTAGTAC+TGG - chr6_2:19529577-19529596 Msa0911990:CDS 35.0%
! TCAGATCTTAGTATTGGAAG+AGG - chr6_2:19529682-19529701 Msa0911990:CDS 35.0%
ACAAATGCTGAATTAATCCC+TGG + chr6_2:19529709-19529728 None:intergenic 35.0%
! TTTGGAGTAAAGAACATGGA+TGG + chr6_2:19528630-19528649 None:intergenic 35.0%
CATGTGCAGAACAAAAAGAA+TGG - chr6_2:19528725-19528744 Msa0911990:CDS 35.0%
AAAGAATGGTCAACGAGTAA+TGG - chr6_2:19528739-19528758 Msa0911990:intron 35.0%
!!! AACTTGTGTCATTTTGGTAC+AGG - chr6_2:19528985-19529004 Msa0911990:intron 35.0%
GTTTCTTAACTTATGCCCTA+AGG - chr6_2:19529117-19529136 Msa0911990:intron 35.0%
!! TATGTTAACTTGTGCCCTTA+GGG + chr6_2:19529135-19529154 None:intergenic 35.0%
!! TTATGTTAACTTGTGCCCTT+AGG + chr6_2:19529136-19529155 None:intergenic 35.0%
!! CTGAATGACTTGGTTCTATA+AGG + chr6_2:19529277-19529296 None:intergenic 35.0%
TCATTAACAACTGTCTCTTC+GGG + chr6_2:19529409-19529428 None:intergenic 35.0%
CTCATTAACAACTGTCTCTT+CGG + chr6_2:19529410-19529429 None:intergenic 35.0%
! ACTTTGATAGTTAGGAGAGA+TGG - chr6_2:19529550-19529569 Msa0911990:CDS 35.0%
GTACTAAGATCTGATCTTCT+TGG + chr6_2:19529577-19529596 None:intergenic 35.0%
AGAAGATCAGATCTTAGTAC+TGG - chr6_2:19529577-19529596 Msa0911990:CDS 35.0%
GTACTAAGATCTGATCTTCT+TGG + chr6_2:19529577-19529596 None:intergenic 35.0%
AGAAGATCAGATCTTAGTAC+TGG - chr6_2:19529577-19529596 Msa0911990:CDS 35.0%
! TCAGATCTTAGTATTGGAAG+AGG - chr6_2:19529682-19529701 Msa0911990:CDS 35.0%
ACAAATGCTGAATTAATCCC+TGG + chr6_2:19529709-19529728 None:intergenic 35.0%
GATGCAGAAGAGAATCTTGA+TGG - chr6_2:19528691-19528710 Msa0911990:CDS 40.0%
CATTAACAACTGTCTCTTCG+GGG + chr6_2:19529408-19529427 None:intergenic 40.0%
CGAAGAGACAGTTGTTAATG+AGG - chr6_2:19529408-19529427 Msa0911990:intron 40.0%
! TCAGATCTTAGTACTGGAAG+AGG - chr6_2:19529583-19529602 Msa0911990:CDS 40.0%
! TCAGATCTTAGTACTGGAAG+AGG - chr6_2:19529583-19529602 Msa0911990:CDS 40.0%
GGAAGATCAGATCTTAGTAC+TGG - chr6_2:19529640-19529659 Msa0911990:CDS 40.0%
! TCAGATCTTAGTACTGGAAG+AGG - chr6_2:19529583-19529602 Msa0911990:CDS 40.0%
! TTAGTATTGGAAGAGGTCCA+GGG - chr6_2:19529689-19529708 Msa0911990:CDS 40.0%
GATGCAGAAGAGAATCTTGA+TGG - chr6_2:19528691-19528710 Msa0911990:CDS 40.0%
CATTAACAACTGTCTCTTCG+GGG + chr6_2:19529408-19529427 None:intergenic 40.0%
CGAAGAGACAGTTGTTAATG+AGG - chr6_2:19529408-19529427 Msa0911990:intron 40.0%
! TCAGATCTTAGTACTGGAAG+AGG - chr6_2:19529583-19529602 Msa0911990:CDS 40.0%
! TCAGATCTTAGTACTGGAAG+AGG - chr6_2:19529583-19529602 Msa0911990:CDS 40.0%
GGAAGATCAGATCTTAGTAC+TGG - chr6_2:19529640-19529659 Msa0911990:CDS 40.0%
! TCAGATCTTAGTACTGGAAG+AGG - chr6_2:19529583-19529602 Msa0911990:CDS 40.0%
! TTAGTATTGGAAGAGGTCCA+GGG - chr6_2:19529689-19529708 Msa0911990:CDS 40.0%
TCGGGGACATCCGATAAAAT+TGG + chr6_2:19529391-19529410 None:intergenic 45.0%
! GATCTTAGTACTGGAAGAGG+TGG - chr6_2:19529586-19529605 Msa0911990:CDS 45.0%
! GATCTTAGTACTGGAAGAGG+TGG - chr6_2:19529586-19529605 Msa0911990:CDS 45.0%
! CTTAGTATTGGAAGAGGTCC+AGG - chr6_2:19529688-19529707 Msa0911990:CDS 45.0%
TCGGGGACATCCGATAAAAT+TGG + chr6_2:19529391-19529410 None:intergenic 45.0%
! GATCTTAGTACTGGAAGAGG+TGG - chr6_2:19529586-19529605 Msa0911990:CDS 45.0%
! GATCTTAGTACTGGAAGAGG+TGG - chr6_2:19529586-19529605 Msa0911990:CDS 45.0%
! CTTAGTATTGGAAGAGGTCC+AGG - chr6_2:19529688-19529707 Msa0911990:CDS 45.0%
!!! TTTTTGTTTTTTTTTTATTT+TGG - chr6_2:19528843-19528862 Msa0911990:intron 5.0%
!!! TTTTGTTTTTTTTTTATTTT+GGG - chr6_2:19528844-19528863 Msa0911990:intron 5.0%
!!! TTTTTGTTTTTTTTTTATTT+TGG - chr6_2:19528843-19528862 Msa0911990:intron 5.0%
!!! TTTTGTTTTTTTTTTATTTT+GGG - chr6_2:19528844-19528863 Msa0911990:intron 5.0%
TGTGCATATCATCCTTGCGC+AGG - chr6_2:19529338-19529357 Msa0911990:intron 50.0%
GTGCATATCATCCTTGCGCA+GGG - chr6_2:19529339-19529358 Msa0911990:intron 50.0%
TGCATATCATCCTTGCGCAG+GGG - chr6_2:19529340-19529359 Msa0911990:intron 50.0%
TGTGCATATCATCCTTGCGC+AGG - chr6_2:19529338-19529357 Msa0911990:intron 50.0%
GTGCATATCATCCTTGCGCA+GGG - chr6_2:19529339-19529358 Msa0911990:intron 50.0%
TGCATATCATCCTTGCGCAG+GGG - chr6_2:19529340-19529359 Msa0911990:intron 50.0%
ATTAGCATGACCCCTGCGCA+AGG + chr6_2:19529353-19529372 None:intergenic 55.0%
ATTAGCATGACCCCTGCGCA+AGG + chr6_2:19529353-19529372 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 19528559 19529732 19528559 ID=Msa0911990;Name=Msa0911990
chr6_2 mRNA 19528559 19529732 19528559 ID=Msa0911990-mRNA-1;Parent=Msa0911990;Name=Msa0911990-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0|1|0|1|1|1|2|0|135
chr6_2 exon 19529520 19529732 19529520 ID=Msa0911990-mRNA-1:exon:4030;Parent=Msa0911990-mRNA-1
chr6_2 exon 19528559 19528753 19528559 ID=Msa0911990-mRNA-1:exon:4029;Parent=Msa0911990-mRNA-1
chr6_2 CDS 19529520 19529732 19529520 ID=Msa0911990-mRNA-1:cds;Parent=Msa0911990-mRNA-1
chr6_2 CDS 19528559 19528753 19528559 ID=Msa0911990-mRNA-1:cds;Parent=Msa0911990-mRNA-1
Gene Sequence

>Msa0911990

ATGGAAGAAATATCACCAAATGAAGAAGCAAATGTTACAAATGAGAGGAGTGCCACTTTTTATCTTTACCATCCATGTTCTTTACTCCAAAAATTTCTTGAAACTTTCTTCAAATGTCTAGGGTTTGATGCAGATGCAGAAGAGAATCTTGATGGCAAAGTTCTTGCATGTGCAGAACAAAAAGAATGGTCAACGAGTAATGGTCAAAGTTTTCAACTTATCAGCACTTTGATAGTTAGGAGAGATGGACCAAGAAGATCAGATCTTAGTACTGGAAGAGGTGGACCAAGAAGATCAGATCTTAGTACTGGAAGAGGAAGATCAGATCTTAGTACTGGAAGAGGTGGACGAAGAAGATCAGATCTTAGTATTGGAAGAGGTCCAGGGATTAATTCAGCATTTGTTTAA

Protein sequence

>Msa0911990

MEEISPNEEANVTNERSATFYLYHPCSLLQKFLETFFKCLGFDADAEENLDGKVLACAEQKEWSTSNGQSFQLISTLIVRRDGPRRSDLSTGRGGPRRSDLSTGRGRSDLSTGRGGRRRSDLSIGRGPGINSAFV*