AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0092520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0092520 MtrunA17_Chr3g0143041 82.727 110 19 0 1 110 1 110 2.40e-62 187
Msa0092520 MtrunA17_Chr3g0143031 82.727 110 19 0 1 110 1 110 2.40e-62 187
Msa0092520 MtrunA17_Chr3g0142551 95.349 86 4 0 25 110 1 86 1.72e-54 166
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 51 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGCCTTATCGGGTATAAT+TGG 0.191469 1_2:+75001961 None:intergenic
ATAGAAAGAAAACTTCTTCT+TGG 0.222728 1_2:-75002323 Msa0092520:intron
ATCGGGCATTTGATTTCTTT+TGG 0.267105 1_2:+75002182 None:intergenic
TCCAACGTCTTTGCGTAAAT+CGG 0.324145 1_2:+75002164 None:intergenic
AAATGACATGACTGTTTAGT+TGG 0.326922 1_2:-75001358 Msa0092520:three_prime_UTR
TTCAGTTCTGCTAATCTTTG+TGG 0.351699 1_2:-75001468 Msa0092520:three_prime_UTR
GCAGCCTAGACTCTCTCTAA+TGG 0.370128 1_2:-75002481 Msa0092520:CDS
ATCGCCGCTGCCGTCGCAAA+TGG 0.374917 1_2:-75002448 Msa0092520:CDS
AGGAGCAGTTACTGATAGAT+TGG 0.384018 1_2:-75001615 Msa0092520:three_prime_UTR
GCTCCTTTGGCTTCTTCCCA+TGG 0.393602 1_2:+75002252 None:intergenic
AAACGAGTGGCTGTGGATAT+CGG 0.394066 1_2:+75002945 None:intergenic
CCTCGTCCTCATCATGTTTG+AGG 0.410618 1_2:-75002871 Msa0092520:intron
TCTCTAACAGCATGCATACT+TGG 0.418938 1_2:-75001872 Msa0092520:three_prime_UTR
CAGCCTAGACTCTCTCTAAT+GGG 0.439535 1_2:-75002480 Msa0092520:CDS
AAAGAGGAGCTGTTACTGAT+AGG 0.462033 1_2:-75001751 Msa0092520:three_prime_UTR
CCAACGTCTTTGCGTAAATC+GGG 0.462699 1_2:+75002165 None:intergenic
TCGCCGCTGCCGTCGCAAAT+GGG 0.476476 1_2:-75002447 Msa0092520:intron
TGCTCTTGGCCTGAGAATAG+AGG 0.483132 1_2:-75001635 Msa0092520:three_prime_UTR
ATTCTGTATTATGCAACATC+AGG 0.496837 1_2:+75001428 None:intergenic
GTGTCCAATTATACCCGATA+AGG 0.504935 1_2:-75001965 Msa0092520:CDS
GTAACTGCTCCTCTATTCTC+AGG 0.512200 1_2:+75001626 None:intergenic
AAAAGACTGACACAATTCTC+AGG 0.524137 1_2:+75001563 None:intergenic
AGAGCGTTATGCAATGCTCT+TGG 0.524652 1_2:-75001649 Msa0092520:three_prime_UTR
TTACCCATTTGCGACGGCAG+CGG 0.526111 1_2:+75002444 None:intergenic
TGAAAACAAACGAGTGGCTG+TGG 0.532751 1_2:+75002938 None:intergenic
GATGGAGAGCTTCTTTATGA+TGG 0.535629 1_2:-75002212 Msa0092520:CDS
TGCATAATACAGAATAGTGT+TGG 0.539389 1_2:-75001420 Msa0092520:three_prime_UTR
TTAGAGAGAGTCTAGGCTGC+TGG 0.541773 1_2:+75002484 None:intergenic
GCGTCTTGAGAAAGGGAAAG+AGG 0.542494 1_2:-75001767 Msa0092520:three_prime_UTR
ACCATGTTATGCTATGCTCT+TGG 0.544304 1_2:-75001586 Msa0092520:three_prime_UTR
GCCAAGAGCATTGCATAACA+TGG 0.547572 1_2:+75001722 None:intergenic
AACTTCTTCTTGGAAAGAAG+CGG 0.547573 1_2:-75002313 Msa0092520:CDS
CCCGATTTACGCAAAGACGT+TGG 0.554712 1_2:-75002165 Msa0092520:CDS
ACCATGTTATGCAATGCTCT+TGG 0.554971 1_2:-75001723 Msa0092520:three_prime_UTR
ACAAAGGAGGATCTTCTCCA+TGG 0.556428 1_2:-75002269 Msa0092520:CDS
CCTCAAACATGATGAGGACG+AGG 0.568612 1_2:+75002871 None:intergenic
TAGATACAATAGACCTCAAG+TGG 0.580845 1_2:-75001987 Msa0092520:CDS
ATTTACGCAAAGACGTTGGA+AGG 0.581460 1_2:-75002161 Msa0092520:intron
GGCACCTATTGAACAAAAGT+AGG 0.586293 1_2:+75002966 None:intergenic
GTGCCCATTAGAGAGAGTCT+AGG 0.590956 1_2:+75002477 None:intergenic
AAAATACCTCAAACATGATG+AGG 0.597319 1_2:+75002865 None:intergenic
AAAAGTAGAAAGCACAAAGG+AGG 0.607683 1_2:-75002282 Msa0092520:CDS
CAAAGGAGGATCTTCTCCAT+GGG 0.608293 1_2:-75002268 Msa0092520:CDS
TCTTCTTGGAAAGAAGCGGA+AGG 0.619915 1_2:-75002309 Msa0092520:CDS
GTATAATTGGACACCACTTG+AGG 0.631367 1_2:+75001974 None:intergenic
GCCAAGAGCATAGCATAACA+TGG 0.648870 1_2:+75001585 None:intergenic
GAACTCTGAAAACAAACGAG+TGG 0.666183 1_2:+75002932 None:intergenic
TCTCCATGGGAAGAAGCCAA+AGG 0.672590 1_2:-75002255 Msa0092520:CDS
GTTGAATTACCCATTTGCGA+CGG 0.680657 1_2:+75002438 None:intergenic
AAACATGATGAGGACGAGGG+AGG 0.707041 1_2:+75002875 None:intergenic
CTCAAACATGATGAGGACGA+GGG 0.717622 1_2:+75002872 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAATAAATCAATTCAA+AGG + chr1_2:75001561-75001580 None:intergenic 10.0%
!!! TTTATTTTTATATTTTGTGA+TGG - chr1_2:75001570-75001589 Msa0092520:three_prime_UTR 10.0%
!! AAAATAATAACAATAAAATG+AGG + chr1_2:75001736-75001755 None:intergenic 10.0%
!! ATAAAAATAAATCAATTCAA+AGG + chr1_2:75001561-75001580 None:intergenic 10.0%
!!! TTTATTTTTATATTTTGTGA+TGG - chr1_2:75001570-75001589 Msa0092520:three_prime_UTR 10.0%
!! AAAATAATAACAATAAAATG+AGG + chr1_2:75001736-75001755 None:intergenic 10.0%
!! AACAACTAATTAATTAAAAG+TGG + chr1_2:75001773-75001792 None:intergenic 15.0%
!! AACAACTAATTAATTAAAAG+TGG + chr1_2:75001773-75001792 None:intergenic 15.0%
!! AACTAGAATTAGAATTGAAA+AGG + chr1_2:75001472-75001491 None:intergenic 20.0%
!!! TTATATTTTGTGATGGAATT+AGG - chr1_2:75001577-75001596 Msa0092520:three_prime_UTR 20.0%
!!! TATATTTTGTGATGGAATTA+GGG - chr1_2:75001578-75001597 Msa0092520:three_prime_UTR 20.0%
!!! TGATTTTCTTTTTATTTGCA+AGG - chr1_2:75001646-75001665 Msa0092520:three_prime_UTR 20.0%
!!! GATTTTCTTTTTATTTGCAA+GGG - chr1_2:75001647-75001666 Msa0092520:three_prime_UTR 20.0%
!! ATTAATTAGTTGTTCTCTAT+TGG - chr1_2:75001779-75001798 Msa0092520:three_prime_UTR 20.0%
!! ATACTTATGAAAATAGGATT+AGG + chr1_2:75001894-75001913 None:intergenic 20.0%
!! AGCAAAATACTTATGAAAAT+AGG + chr1_2:75001900-75001919 None:intergenic 20.0%
!! TGAAATAATAAGAATCCAAA+GGG + chr1_2:75001956-75001975 None:intergenic 20.0%
!! ATGAAATAATAAGAATCCAA+AGG + chr1_2:75001957-75001976 None:intergenic 20.0%
!!! TGTGCATATTTTAATTCTTT+AGG - chr1_2:75002403-75002422 Msa0092520:intron 20.0%
!! AACTAGAATTAGAATTGAAA+AGG + chr1_2:75001472-75001491 None:intergenic 20.0%
!!! TTATATTTTGTGATGGAATT+AGG - chr1_2:75001577-75001596 Msa0092520:three_prime_UTR 20.0%
!!! TATATTTTGTGATGGAATTA+GGG - chr1_2:75001578-75001597 Msa0092520:three_prime_UTR 20.0%
!!! TGATTTTCTTTTTATTTGCA+AGG - chr1_2:75001646-75001665 Msa0092520:three_prime_UTR 20.0%
!!! GATTTTCTTTTTATTTGCAA+GGG - chr1_2:75001647-75001666 Msa0092520:three_prime_UTR 20.0%
!! ATTAATTAGTTGTTCTCTAT+TGG - chr1_2:75001779-75001798 Msa0092520:three_prime_UTR 20.0%
!! ATACTTATGAAAATAGGATT+AGG + chr1_2:75001894-75001913 None:intergenic 20.0%
!! AGCAAAATACTTATGAAAAT+AGG + chr1_2:75001900-75001919 None:intergenic 20.0%
!! TGAAATAATAAGAATCCAAA+GGG + chr1_2:75001956-75001975 None:intergenic 20.0%
!! ATGAAATAATAAGAATCCAA+AGG + chr1_2:75001957-75001976 None:intergenic 20.0%
!!! TGTGCATATTTTAATTCTTT+AGG - chr1_2:75002403-75002422 Msa0092520:intron 20.0%
! TTCTCAAATTTGTGAGATTA+GGG - chr1_2:75001528-75001547 Msa0092520:three_prime_UTR 25.0%
! ACAATAAAATGAGGAACATA+TGG + chr1_2:75001727-75001746 None:intergenic 25.0%
! CTCTATTGGAATTTATTCAA+AGG - chr1_2:75001793-75001812 Msa0092520:three_prime_UTR 25.0%
! ATAGAAAGAAAACTTCTTCT+TGG - chr1_2:75001984-75002003 Msa0092520:CDS 25.0%
!!! GTTTCTTCTTTTGTTTCATA+TGG - chr1_2:75002264-75002283 Msa0092520:CDS 25.0%
! TTCTCAAATTTGTGAGATTA+GGG - chr1_2:75001528-75001547 Msa0092520:three_prime_UTR 25.0%
! ACAATAAAATGAGGAACATA+TGG + chr1_2:75001727-75001746 None:intergenic 25.0%
! CTCTATTGGAATTTATTCAA+AGG - chr1_2:75001793-75001812 Msa0092520:three_prime_UTR 25.0%
! ATAGAAAGAAAACTTCTTCT+TGG - chr1_2:75001984-75002003 Msa0092520:CDS 25.0%
!!! GTTTCTTCTTTTGTTTCATA+TGG - chr1_2:75002264-75002283 Msa0092520:CDS 25.0%
AAAATACCTCAAACATGATG+AGG + chr1_2:75001445-75001464 None:intergenic 30.0%
CTTCTCAAATTTGTGAGATT+AGG - chr1_2:75001527-75001546 Msa0092520:three_prime_UTR 30.0%
! AGGGTTTCAAACTTTTGAAT+TGG - chr1_2:75001597-75001616 Msa0092520:three_prime_UTR 30.0%
! GGGTTTCAAACTTTTGAATT+GGG - chr1_2:75001598-75001617 Msa0092520:three_prime_UTR 30.0%
! GGTTTCAAACTTTTGAATTG+GGG - chr1_2:75001599-75001618 Msa0092520:three_prime_UTR 30.0%
CAAATAATAACTTCACCCTT+TGG - chr1_2:75001938-75001957 Msa0092520:exon 30.0%
!! CAAAAAGTAGAAAGTACTGA+TGG - chr1_2:75002077-75002096 Msa0092520:intron 30.0%
AAGAGAAAAACTTGCCTTAT+CGG + chr1_2:75002359-75002378 None:intergenic 30.0%
!!! CTTTGTTTTGTATTCTGTGT+TGG - chr1_2:75002814-75002833 Msa0092520:intron 30.0%
ATTCTGTATTATGCAACATC+AGG + chr1_2:75002882-75002901 None:intergenic 30.0%
TGCATAATACAGAATAGTGT+TGG - chr1_2:75002887-75002906 Msa0092520:five_prime_UTR 30.0%
AAATGACATGACTGTTTAGT+TGG - chr1_2:75002949-75002968 Msa0092520:five_prime_UTR 30.0%
AAAATACCTCAAACATGATG+AGG + chr1_2:75001445-75001464 None:intergenic 30.0%
CTTCTCAAATTTGTGAGATT+AGG - chr1_2:75001527-75001546 Msa0092520:three_prime_UTR 30.0%
! AGGGTTTCAAACTTTTGAAT+TGG - chr1_2:75001597-75001616 Msa0092520:three_prime_UTR 30.0%
! GGGTTTCAAACTTTTGAATT+GGG - chr1_2:75001598-75001617 Msa0092520:three_prime_UTR 30.0%
! GGTTTCAAACTTTTGAATTG+GGG - chr1_2:75001599-75001618 Msa0092520:three_prime_UTR 30.0%
CAAATAATAACTTCACCCTT+TGG - chr1_2:75001938-75001957 Msa0092520:exon 30.0%
!! CAAAAAGTAGAAAGTACTGA+TGG - chr1_2:75002077-75002096 Msa0092520:intron 30.0%
AAGAGAAAAACTTGCCTTAT+CGG + chr1_2:75002359-75002378 None:intergenic 30.0%
!!! CTTTGTTTTGTATTCTGTGT+TGG - chr1_2:75002814-75002833 Msa0092520:intron 30.0%
ATTCTGTATTATGCAACATC+AGG + chr1_2:75002882-75002901 None:intergenic 30.0%
TGCATAATACAGAATAGTGT+TGG - chr1_2:75002887-75002906 Msa0092520:five_prime_UTR 30.0%
AAATGACATGACTGTTTAGT+TGG - chr1_2:75002949-75002968 Msa0092520:five_prime_UTR 30.0%
! TCGTTTTCTACATTCTTCGT+TGG - chr1_2:75001400-75001419 Msa0092520:three_prime_UTR 35.0%
CAGATTGACACACACAAAAT+TGG + chr1_2:75001628-75001647 None:intergenic 35.0%
! TTGCAAGGGTTTTTGTTGAT+TGG - chr1_2:75001661-75001680 Msa0092520:intron 35.0%
GAGGAACATATGGAGTATAT+AGG + chr1_2:75001717-75001736 None:intergenic 35.0%
AACTTCTTCTTGGAAAGAAG+CGG - chr1_2:75001994-75002013 Msa0092520:CDS 35.0%
! GCAAAAAGTAGAAAGCACAA+AGG - chr1_2:75002022-75002041 Msa0092520:intron 35.0%
! AAAAGTAGAAAGCACAAAGG+AGG - chr1_2:75002025-75002044 Msa0092520:intron 35.0%
!!! CTTTCTACTTTTTGCTCCTT+TGG + chr1_2:75002017-75002036 None:intergenic 35.0%
!! ATCGGGCATTTGATTTCTTT+TGG + chr1_2:75002128-75002147 None:intergenic 35.0%
GATCAAATAACCTCAGCTTA+TGG - chr1_2:75002239-75002258 Msa0092520:CDS 35.0%
TAGATACAATAGACCTCAAG+TGG - chr1_2:75002320-75002339 Msa0092520:CDS 35.0%
AGAGAAAAACTTGCCTTATC+GGG + chr1_2:75002358-75002377 None:intergenic 35.0%
AGCATGAAGAGAAAAAGAAG+CGG - chr1_2:75002491-75002510 Msa0092520:CDS 35.0%
!! AAAAAGAGCGTCTTGAGAAA+GGG - chr1_2:75002533-75002552 Msa0092520:intron 35.0%
AACGCTCTAATAGCATAACA+TGG + chr1_2:75002646-75002665 None:intergenic 35.0%
AAAAGACTGACACAATTCTC+AGG + chr1_2:75002747-75002766 None:intergenic 35.0%
TTCAGTTCTGCTAATCTTTG+TGG - chr1_2:75002839-75002858 Msa0092520:intron 35.0%
! TCGTTTTCTACATTCTTCGT+TGG - chr1_2:75001400-75001419 Msa0092520:three_prime_UTR 35.0%
CAGATTGACACACACAAAAT+TGG + chr1_2:75001628-75001647 None:intergenic 35.0%
! TTGCAAGGGTTTTTGTTGAT+TGG - chr1_2:75001661-75001680 Msa0092520:intron 35.0%
GAGGAACATATGGAGTATAT+AGG + chr1_2:75001717-75001736 None:intergenic 35.0%
AACTTCTTCTTGGAAAGAAG+CGG - chr1_2:75001994-75002013 Msa0092520:CDS 35.0%
! GCAAAAAGTAGAAAGCACAA+AGG - chr1_2:75002022-75002041 Msa0092520:intron 35.0%
! AAAAGTAGAAAGCACAAAGG+AGG - chr1_2:75002025-75002044 Msa0092520:intron 35.0%
!!! CTTTCTACTTTTTGCTCCTT+TGG + chr1_2:75002017-75002036 None:intergenic 35.0%
!! ATCGGGCATTTGATTTCTTT+TGG + chr1_2:75002128-75002147 None:intergenic 35.0%
GATCAAATAACCTCAGCTTA+TGG - chr1_2:75002239-75002258 Msa0092520:CDS 35.0%
TAGATACAATAGACCTCAAG+TGG - chr1_2:75002320-75002339 Msa0092520:CDS 35.0%
AGAGAAAAACTTGCCTTATC+GGG + chr1_2:75002358-75002377 None:intergenic 35.0%
AGCATGAAGAGAAAAAGAAG+CGG - chr1_2:75002491-75002510 Msa0092520:CDS 35.0%
!! AAAAAGAGCGTCTTGAGAAA+GGG - chr1_2:75002533-75002552 Msa0092520:intron 35.0%
AACGCTCTAATAGCATAACA+TGG + chr1_2:75002646-75002665 None:intergenic 35.0%
AAAAGACTGACACAATTCTC+AGG + chr1_2:75002747-75002766 None:intergenic 35.0%
TTCAGTTCTGCTAATCTTTG+TGG - chr1_2:75002839-75002858 Msa0092520:intron 35.0%
GGCACCTATTGAACAAAAGT+AGG + chr1_2:75001344-75001363 None:intergenic 40.0%
! GAACTCTGAAAACAAACGAG+TGG + chr1_2:75001378-75001397 None:intergenic 40.0%
GTTGAATTACCCATTTGCGA+CGG + chr1_2:75001872-75001891 None:intergenic 40.0%
GATGGAGAGCTTCTTTATGA+TGG - chr1_2:75002095-75002114 Msa0092520:intron 40.0%
TCCAACGTCTTTGCGTAAAT+CGG + chr1_2:75002146-75002165 None:intergenic 40.0%
ATTTACGCAAAGACGTTGGA+AGG - chr1_2:75002146-75002165 Msa0092520:intron 40.0%
GAAGAAACAACCATAAGCTG+AGG + chr1_2:75002252-75002271 None:intergenic 40.0%
GTATAATTGGACACCACTTG+AGG + chr1_2:75002336-75002355 None:intergenic 40.0%
GTGTCCAATTATACCCGATA+AGG - chr1_2:75002342-75002361 Msa0092520:intron 40.0%
CTTGCCTTATCGGGTATAAT+TGG + chr1_2:75002349-75002368 None:intergenic 40.0%
TCTCTAACAGCATGCATACT+TGG - chr1_2:75002435-75002454 Msa0092520:intron 40.0%
ATGAAGAGAAAAAGAAGCGG+TGG - chr1_2:75002494-75002513 Msa0092520:CDS 40.0%
!! GAAAAAGAGCGTCTTGAGAA+AGG - chr1_2:75002532-75002551 Msa0092520:intron 40.0%
AAAGAGGAGCTGTTACTGAT+AGG - chr1_2:75002556-75002575 Msa0092520:intron 40.0%
ACCATGTTATGCAATGCTCT+TGG - chr1_2:75002584-75002603 Msa0092520:intron 40.0%
ATAGAGGAGCAGTTACTGAT+AGG - chr1_2:75002614-75002633 Msa0092520:intron 40.0%
AGGAGCAGTTACTGATAGAT+TGG - chr1_2:75002692-75002711 Msa0092520:intron 40.0%
ACCATGTTATGCTATGCTCT+TGG - chr1_2:75002721-75002740 Msa0092520:intron 40.0%
GGCACCTATTGAACAAAAGT+AGG + chr1_2:75001344-75001363 None:intergenic 40.0%
! GAACTCTGAAAACAAACGAG+TGG + chr1_2:75001378-75001397 None:intergenic 40.0%
GTTGAATTACCCATTTGCGA+CGG + chr1_2:75001872-75001891 None:intergenic 40.0%
GATGGAGAGCTTCTTTATGA+TGG - chr1_2:75002095-75002114 Msa0092520:intron 40.0%
TCCAACGTCTTTGCGTAAAT+CGG + chr1_2:75002146-75002165 None:intergenic 40.0%
ATTTACGCAAAGACGTTGGA+AGG - chr1_2:75002146-75002165 Msa0092520:intron 40.0%
GAAGAAACAACCATAAGCTG+AGG + chr1_2:75002252-75002271 None:intergenic 40.0%
GTATAATTGGACACCACTTG+AGG + chr1_2:75002336-75002355 None:intergenic 40.0%
GTGTCCAATTATACCCGATA+AGG - chr1_2:75002342-75002361 Msa0092520:intron 40.0%
CTTGCCTTATCGGGTATAAT+TGG + chr1_2:75002349-75002368 None:intergenic 40.0%
TCTCTAACAGCATGCATACT+TGG - chr1_2:75002435-75002454 Msa0092520:intron 40.0%
ATGAAGAGAAAAAGAAGCGG+TGG - chr1_2:75002494-75002513 Msa0092520:CDS 40.0%
!! GAAAAAGAGCGTCTTGAGAA+AGG - chr1_2:75002532-75002551 Msa0092520:intron 40.0%
AAAGAGGAGCTGTTACTGAT+AGG - chr1_2:75002556-75002575 Msa0092520:intron 40.0%
ACCATGTTATGCAATGCTCT+TGG - chr1_2:75002584-75002603 Msa0092520:intron 40.0%
ATAGAGGAGCAGTTACTGAT+AGG - chr1_2:75002614-75002633 Msa0092520:intron 40.0%
AGGAGCAGTTACTGATAGAT+TGG - chr1_2:75002692-75002711 Msa0092520:intron 40.0%
ACCATGTTATGCTATGCTCT+TGG - chr1_2:75002721-75002740 Msa0092520:intron 40.0%
AAACGAGTGGCTGTGGATAT+CGG + chr1_2:75001365-75001384 None:intergenic 45.0%
TGAAAACAAACGAGTGGCTG+TGG + chr1_2:75001372-75001391 None:intergenic 45.0%
CTCAAACATGATGAGGACGA+GGG + chr1_2:75001438-75001457 None:intergenic 45.0%
CAGCCTAGACTCTCTCTAAT+GGG - chr1_2:75001827-75001846 Msa0092520:three_prime_UTR 45.0%
TCTTCTTGGAAAGAAGCGGA+AGG - chr1_2:75001998-75002017 Msa0092520:CDS 45.0%
ACAAAGGAGGATCTTCTCCA+TGG - chr1_2:75002038-75002057 Msa0092520:intron 45.0%
CAAAGGAGGATCTTCTCCAT+GGG - chr1_2:75002039-75002058 Msa0092520:intron 45.0%
CCAACGTCTTTGCGTAAATC+GGG + chr1_2:75002145-75002164 None:intergenic 45.0%
!! GCCAAGAGCATTGCATAACA+TGG + chr1_2:75002588-75002607 None:intergenic 45.0%
GTAACTGCTCCTCTATTCTC+AGG + chr1_2:75002610-75002629 None:intergenic 45.0%
! AGAGCGTTATGCAATGCTCT+TGG - chr1_2:75002658-75002677 Msa0092520:intron 45.0%
GTAACTGCTCCTCTATTCTC+AGG + chr1_2:75002610-75002629 None:intergenic 45.0%
! GCCAAGAGCATAGCATAACA+TGG + chr1_2:75002725-75002744 None:intergenic 45.0%
AAACGAGTGGCTGTGGATAT+CGG + chr1_2:75001365-75001384 None:intergenic 45.0%
TGAAAACAAACGAGTGGCTG+TGG + chr1_2:75001372-75001391 None:intergenic 45.0%
CTCAAACATGATGAGGACGA+GGG + chr1_2:75001438-75001457 None:intergenic 45.0%
CAGCCTAGACTCTCTCTAAT+GGG - chr1_2:75001827-75001846 Msa0092520:three_prime_UTR 45.0%
TCTTCTTGGAAAGAAGCGGA+AGG - chr1_2:75001998-75002017 Msa0092520:CDS 45.0%
ACAAAGGAGGATCTTCTCCA+TGG - chr1_2:75002038-75002057 Msa0092520:intron 45.0%
CAAAGGAGGATCTTCTCCAT+GGG - chr1_2:75002039-75002058 Msa0092520:intron 45.0%
CCAACGTCTTTGCGTAAATC+GGG + chr1_2:75002145-75002164 None:intergenic 45.0%
!! GCCAAGAGCATTGCATAACA+TGG + chr1_2:75002588-75002607 None:intergenic 45.0%
GTAACTGCTCCTCTATTCTC+AGG + chr1_2:75002610-75002629 None:intergenic 45.0%
! AGAGCGTTATGCAATGCTCT+TGG - chr1_2:75002658-75002677 Msa0092520:intron 45.0%
GTAACTGCTCCTCTATTCTC+AGG + chr1_2:75002610-75002629 None:intergenic 45.0%
! GCCAAGAGCATAGCATAACA+TGG + chr1_2:75002725-75002744 None:intergenic 45.0%
AAACATGATGAGGACGAGGG+AGG + chr1_2:75001435-75001454 None:intergenic 50.0%
CCTCAAACATGATGAGGACG+AGG + chr1_2:75001439-75001458 None:intergenic 50.0%
CCTCGTCCTCATCATGTTTG+AGG - chr1_2:75001436-75001455 Msa0092520:three_prime_UTR 50.0%
TTAGAGAGAGTCTAGGCTGC+TGG + chr1_2:75001826-75001845 None:intergenic 50.0%
GCAGCCTAGACTCTCTCTAA+TGG - chr1_2:75001826-75001845 Msa0092520:three_prime_UTR 50.0%
GTGCCCATTAGAGAGAGTCT+AGG + chr1_2:75001833-75001852 None:intergenic 50.0%
TCTCCATGGGAAGAAGCCAA+AGG - chr1_2:75002052-75002071 Msa0092520:intron 50.0%
CCCGATTTACGCAAAGACGT+TGG - chr1_2:75002142-75002161 Msa0092520:intron 50.0%
GCGTCTTGAGAAAGGGAAAG+AGG - chr1_2:75002540-75002559 Msa0092520:intron 50.0%
TGCTCTTGGCCTGAGAATAG+AGG - chr1_2:75002598-75002617 Msa0092520:intron 50.0%
TGCTCTTGGCCTGAGAATAG+AGG - chr1_2:75002598-75002617 Msa0092520:intron 50.0%
AAACATGATGAGGACGAGGG+AGG + chr1_2:75001435-75001454 None:intergenic 50.0%
CCTCAAACATGATGAGGACG+AGG + chr1_2:75001439-75001458 None:intergenic 50.0%
CCTCGTCCTCATCATGTTTG+AGG - chr1_2:75001436-75001455 Msa0092520:three_prime_UTR 50.0%
TTAGAGAGAGTCTAGGCTGC+TGG + chr1_2:75001826-75001845 None:intergenic 50.0%
GCAGCCTAGACTCTCTCTAA+TGG - chr1_2:75001826-75001845 Msa0092520:three_prime_UTR 50.0%
GTGCCCATTAGAGAGAGTCT+AGG + chr1_2:75001833-75001852 None:intergenic 50.0%
TCTCCATGGGAAGAAGCCAA+AGG - chr1_2:75002052-75002071 Msa0092520:intron 50.0%
CCCGATTTACGCAAAGACGT+TGG - chr1_2:75002142-75002161 Msa0092520:intron 50.0%
GCGTCTTGAGAAAGGGAAAG+AGG - chr1_2:75002540-75002559 Msa0092520:intron 50.0%
TGCTCTTGGCCTGAGAATAG+AGG - chr1_2:75002598-75002617 Msa0092520:intron 50.0%
TGCTCTTGGCCTGAGAATAG+AGG - chr1_2:75002598-75002617 Msa0092520:intron 50.0%
TTACCCATTTGCGACGGCAG+CGG + chr1_2:75001866-75001885 None:intergenic 55.0%
! GCTCCTTTGGCTTCTTCCCA+TGG + chr1_2:75002058-75002077 None:intergenic 55.0%
TTACCCATTTGCGACGGCAG+CGG + chr1_2:75001866-75001885 None:intergenic 55.0%
! GCTCCTTTGGCTTCTTCCCA+TGG + chr1_2:75002058-75002077 None:intergenic 55.0%
ATCGCCGCTGCCGTCGCAAA+TGG - chr1_2:75001859-75001878 Msa0092520:three_prime_UTR 65.0%
TCGCCGCTGCCGTCGCAAAT+GGG - chr1_2:75001860-75001879 Msa0092520:three_prime_UTR 65.0%
ATCGCCGCTGCCGTCGCAAA+TGG - chr1_2:75001859-75001878 Msa0092520:three_prime_UTR 65.0%
TCGCCGCTGCCGTCGCAAAT+GGG - chr1_2:75001860-75001879 Msa0092520:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 75001338 75002991 75001338 ID=Msa0092520;Name=Msa0092520
chr1_2 mRNA 75001338 75002991 75001338 ID=Msa0092520-mRNA-1;Parent=Msa0092520;Name=Msa0092520-mRNA-1;_AED=0.14;_eAED=0.14;_QI=111|1|1|1|0.75|0.6|5|552|115
chr1_2 exon 75001338 75001669 75001338 ID=Msa0092520-mRNA-1:exon:26452;Parent=Msa0092520-mRNA-1
chr1_2 exon 75001719 75002030 75001719 ID=Msa0092520-mRNA-1:exon:26451;Parent=Msa0092520-mRNA-1
chr1_2 exon 75002162 75002341 75002162 ID=Msa0092520-mRNA-1:exon:26450;Parent=Msa0092520-mRNA-1
chr1_2 exon 75002448 75002514 75002448 ID=Msa0092520-mRNA-1:exon:26449;Parent=Msa0092520-mRNA-1
chr1_2 exon 75002872 75002991 75002872 ID=Msa0092520-mRNA-1:exon:26448;Parent=Msa0092520-mRNA-1
chr1_2 five_prime_UTR 75002881 75002991 75002881 ID=Msa0092520-mRNA-1:five_prime_utr;Parent=Msa0092520-mRNA-1
chr1_2 CDS 75002872 75002880 75002872 ID=Msa0092520-mRNA-1:cds;Parent=Msa0092520-mRNA-1
chr1_2 CDS 75002448 75002514 75002448 ID=Msa0092520-mRNA-1:cds;Parent=Msa0092520-mRNA-1
chr1_2 CDS 75002162 75002341 75002162 ID=Msa0092520-mRNA-1:cds;Parent=Msa0092520-mRNA-1
chr1_2 CDS 75001939 75002030 75001939 ID=Msa0092520-mRNA-1:cds;Parent=Msa0092520-mRNA-1
chr1_2 three_prime_UTR 75001719 75001938 75001719 ID=Msa0092520-mRNA-1:three_prime_utr;Parent=Msa0092520-mRNA-1
chr1_2 three_prime_UTR 75001338 75001669 75001338 ID=Msa0092520-mRNA-1:three_prime_utr;Parent=Msa0092520-mRNA-1
Gene Sequence

>Msa0092520

ATGTTTGAGGAACGTTACCAGCAGCCTAGACTCTCTCTAATGGGCACTTCACGATCGCCGCTGCCGTCGCAAATGGAAAGAAAACTTCTTCTTGGAAAGAAGCGGAAGGAGCAAAAAGTAGAAAGCACAAAGGAGGATCTTCTCCATGGGAAGAAGCCAAAGGAGCAAAAAGTAGAAAGTACTGATGGAGAGCTTCTTTATGATGGTGAGAAGCCAAAAGAAATCAAATGCCCGATTTACGCAAAGACGTTGGAAGTCTATAACAAGCTGAAAGATATAGATACAATAGACCTCAAGTGGTGTCCAATTATACCCGATAAGGCAAGTTTTTCTCTTAAACTTTCATAA
Protein sequence

>Msa0092520

MFEERYQQPRLSLMGTSRSPLPSQMERKLLLGKKRKEQKVESTKEDLLHGKKPKEQKVESTDGELLYDGEKPKEIKCPIYAKTLEVYNKLKDIDTIDLKWCPIIPDKASFSLKLS*