Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0170000 | XP_003590795.1 | 88.571 | 70 | 8 | 0 | 1 | 70 | 1 | 70 | 7.57e-37 | 128 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0170000 | sp|P19873|ITH5_CUCMA | 60.294 | 68 | 27 | 0 | 3 | 70 | 1 | 68 | 1.57e-23 | 87.0 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0170000 | G7I3Z2 | 88.571 | 70 | 8 | 0 | 1 | 70 | 1 | 70 | 3.62e-37 | 128 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0077380 | Msa0170000 | 0.821051 | 4.957286e-53 | -8.615850e-47 |
| Msa0117520 | Msa0170000 | 0.816820 | 4.539725e-52 | -8.615850e-47 |
| Msa0160960 | Msa0170000 | 0.803525 | 3.343624e-49 | -8.615850e-47 |
| Msa0170000 | Msa0321690 | 0.801033 | 1.089579e-48 | -8.615850e-47 |
| Msa0170000 | Msa0331400 | 0.811191 | 7.922358e-51 | -8.615850e-47 |
| Msa0170000 | Msa0471900 | 0.804282 | 2.328033e-49 | -8.615850e-47 |
| Msa0170000 | Msa0518170 | 0.806617 | 7.540809e-50 | -8.615850e-47 |
| Msa0170000 | Msa0519160 | 0.807787 | 4.261606e-50 | -8.615850e-47 |
| Msa0170000 | Msa0604290 | 0.805773 | 1.135171e-49 | -8.615850e-47 |
| Msa0170000 | Msa0645530 | 0.806047 | 9.942078e-50 | -8.615850e-47 |
| Msa0170000 | Msa0657120 | 0.806795 | 6.914563e-50 | -8.615850e-47 |
| Msa0170000 | Msa0685090 | 0.807908 | 4.016386e-50 | -8.615850e-47 |
| Msa0170000 | Msa0750850 | 0.802287 | 6.026316e-49 | -8.615850e-47 |
| Msa0170000 | Msa0994370 | 0.839301 | 1.719954e-57 | -8.615850e-47 |
| Msa0170000 | Msa1013070 | 0.814191 | 1.747113e-51 | -8.615850e-47 |
| Msa0170000 | Msa1054510 | 0.806421 | 8.294253e-50 | -8.615850e-47 |
| Msa0170000 | Msa1107200 | 0.809073 | 2.265839e-50 | -8.615850e-47 |
| Msa0170000 | Msa1109810 | 0.834320 | 3.209016e-56 | -8.615850e-47 |
| Msa0170000 | Msa1140230 | 0.802314 | 5.951201e-49 | -8.615850e-47 |
| Msa0170000 | Msa1270660 | 0.808195 | 3.488741e-50 | -8.615850e-47 |
| Msa0170000 | Msa1270730 | 0.806055 | 9.902655e-50 | -8.615850e-47 |
| Msa0170000 | Msa1332050 | 0.800261 | 1.566168e-48 | -8.615850e-47 |
| Msa0170000 | Msa1335230 | 0.811127 | 8.177865e-51 | -8.615850e-47 |
| Msa0170000 | Msa1400930 | 0.804328 | 2.277302e-49 | -8.615850e-47 |
| Msa0170000 | Msa1437730 | 0.809218 | 2.109554e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0170000 | MtrunA17_Chr1g0187161 | 88.571 | 70 | 8 | 0 | 1 | 70 | 1 | 70 | 6.97e-41 | 128 |
| Msa0170000 | MtrunA17_Chr1g0187221 | 83.824 | 68 | 11 | 0 | 3 | 70 | 5 | 72 | 9.65e-36 | 115 |
| Msa0170000 | MtrunA17_Chr5g0442291 | 82.857 | 70 | 12 | 0 | 1 | 70 | 1 | 70 | 3.98e-35 | 113 |
| Msa0170000 | MtrunA17_Chr1g0187201 | 80.882 | 68 | 13 | 0 | 3 | 70 | 4 | 71 | 1.26e-32 | 107 |
| Msa0170000 | MtrunA17_Chr1g0184411 | 55.714 | 70 | 31 | 0 | 1 | 70 | 1 | 70 | 1.18e-22 | 82.0 |
| Msa0170000 | MtrunA17_Chr1g0184401 | 57.576 | 66 | 28 | 0 | 5 | 70 | 8 | 73 | 6.14e-22 | 80.5 |
| Msa0170000 | MtrunA17_Chr5g0419531 | 55.224 | 67 | 30 | 0 | 4 | 70 | 3 | 69 | 9.67e-20 | 74.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0170000 | AT2G38870.1 | 52.857 | 70 | 33 | 0 | 1 | 70 | 1 | 70 | 1.69e-21 | 79.3 |
| Msa0170000 | AT5G43580.1 | 55.556 | 72 | 30 | 1 | 1 | 70 | 28 | 99 | 3.45e-21 | 79.7 |
| Msa0170000 | AT3G46860.1 | 46.032 | 63 | 34 | 0 | 8 | 70 | 23 | 85 | 1.45e-13 | 59.7 |
| Msa0170000 | AT5G43570.1 | 47.945 | 73 | 32 | 1 | 1 | 67 | 1 | 73 | 9.67e-13 | 58.2 |
| Msa0170000 | AT5G43570.2 | 51.667 | 60 | 29 | 0 | 8 | 67 | 24 | 83 | 9.92e-12 | 55.8 |
| Msa0170000 | AT2G38900.1 | 42.188 | 64 | 37 | 0 | 7 | 70 | 10 | 73 | 1.23e-11 | 54.7 |
| Msa0170000 | AT2G38900.2 | 42.188 | 64 | 37 | 0 | 7 | 70 | 25 | 88 | 1.71e-11 | 54.7 |
Find 33 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGTGACAGGGTTTGGGTTT+GGG | 0.259063 | 1_4:+53562633 | Msa0170000:CDS |
| ACTTCCGCTGTGACAGGGTT+TGG | 0.267379 | 1_4:+53562626 | Msa0170000:CDS |
| CTTCCGCTGTGACAGGGTTT+GGG | 0.309008 | 1_4:+53562627 | Msa0170000:CDS |
| GCTGTGACAGGGTTTGGGTT+TGG | 0.326505 | 1_4:+53562632 | Msa0170000:CDS |
| CCTTTGACTTCCGCTGTGAC+AGG | 0.333094 | 1_4:+53562620 | Msa0170000:CDS |
| ATAAATATAATGTTTCAATA+AGG | 0.376895 | 1_4:+53562740 | Msa0170000:three_prime_UTR |
| ATAGTAGTATACTATAGAAT+TGG | 0.396770 | 1_4:-53562778 | None:intergenic |
| TGGGTTTGGGTTAATAAAGA+TGG | 0.418377 | 1_4:+53562646 | Msa0170000:CDS |
| TACATATTGAAGGATCAAAA+AGG | 0.425186 | 1_4:+53562347 | Msa0170000:five_prime_UTR |
| GAAACATTATATTTATAACT+TGG | 0.439182 | 1_4:-53562733 | None:intergenic |
| CCTTCCACTCCAACCAACTC+TGG | 0.454716 | 1_4:-53562520 | None:intergenic |
| CTTTGACTTCCGCTGTGACA+GGG | 0.463269 | 1_4:+53562621 | Msa0170000:CDS |
| CTGTCACAGCGGAAGTCAAA+GGG | 0.467050 | 1_4:-53562619 | None:intergenic |
| TGTGCTCAGGTAAGAGTTCA+TGG | 0.479878 | 1_4:+53562497 | Msa0170000:intron |
| CAAATAGTCATACATATTGA+AGG | 0.500193 | 1_4:+53562337 | Msa0170000:five_prime_UTR |
| GATGCCATCATTGTGCTAGA+AGG | 0.504821 | 1_4:+53562586 | Msa0170000:CDS |
| CCAGAGTTGGTTGGAGTGGA+AGG | 0.515293 | 1_4:+53562520 | Msa0170000:CDS |
| GTTGGTTGGAGTGGAAGGAA+AGG | 0.527079 | 1_4:+53562525 | Msa0170000:CDS |
| AGTTCCTTCTAGCACAATGA+TGG | 0.538007 | 1_4:-53562590 | None:intergenic |
| AATTCAGAGGGAGAATCCAT+TGG | 0.542280 | 1_4:+53562561 | Msa0170000:CDS |
| ATGGCCAGAGTTGGTTGGAG+TGG | 0.545068 | 1_4:+53562516 | Msa0170000:CDS |
| CCTGTCACAGCGGAAGTCAA+AGG | 0.545493 | 1_4:-53562620 | None:intergenic |
| GTGATATATAATGCATTGCA+AGG | 0.551843 | 1_4:-53562278 | None:intergenic |
| TAAGAGTTCATGGCCAGAGT+TGG | 0.563662 | 1_4:+53562507 | Msa0170000:CDS |
| AGTTCATGGCCAGAGTTGGT+TGG | 0.565712 | 1_4:+53562511 | Msa0170000:CDS |
| TAAAGATGGAATTGTCACTC+AGG | 0.589757 | 1_4:+53562660 | Msa0170000:CDS |
| AGCAGAAGCTACAATTCAGA+GGG | 0.593359 | 1_4:+53562549 | Msa0170000:CDS |
| TAGCAGAAGCTACAATTCAG+AGG | 0.596029 | 1_4:+53562548 | Msa0170000:CDS |
| AAATTGGATAGAAAGTGTCA+TGG | 0.640164 | 1_4:+53562689 | Msa0170000:exon |
| ACAATGATGGCATCAACCAA+TGG | 0.650325 | 1_4:-53562577 | None:intergenic |
| AAAATGTCTGATGAATGCAA+AGG | 0.675933 | 1_4:+53562376 | Msa0170000:exon |
| TGTCACAGCGGAAGTCAAAG+GGG | 0.698463 | 1_4:-53562618 | None:intergenic |
| AAACCCAAACCCTGTCACAG+CGG | 0.730650 | 1_4:-53562630 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAATATAATGTTTCAATA+AGG | + | chr1_4:53562740-53562759 | Msa0170000:three_prime_UTR | 10.0% |
| !! | GAAACATTATATTTATAACT+TGG | - | chr1_4:53562736-53562755 | None:intergenic | 15.0% |
| !! | AATTGCAAAAAGAAAGAAAA+AGG | + | chr1_4:53562310-53562329 | Msa0170000:five_prime_UTR | 20.0% |
| !! | ATAGTAGTATACTATAGAAT+TGG | - | chr1_4:53562781-53562800 | None:intergenic | 20.0% |
| ! | CAAATAGTCATACATATTGA+AGG | + | chr1_4:53562337-53562356 | Msa0170000:five_prime_UTR | 25.0% |
| ! | TACATATTGAAGGATCAAAA+AGG | + | chr1_4:53562347-53562366 | Msa0170000:five_prime_UTR | 25.0% |
| !! | AACTTGTAATTCGTTTTCTT+TGG | + | chr1_4:53562848-53562867 | Msa0170000:three_prime_UTR | 25.0% |
| AAAATGTCTGATGAATGCAA+AGG | + | chr1_4:53562376-53562395 | Msa0170000:exon | 30.0% | |
| ! | GACACTTTCTATCCAATTTT+TGG | - | chr1_4:53562688-53562707 | None:intergenic | 30.0% |
| !! | AAATTGGATAGAAAGTGTCA+TGG | + | chr1_4:53562689-53562708 | Msa0170000:exon | 30.0% |
| TCAATATATTGTGTGTGCTC+AGG | + | chr1_4:53562484-53562503 | Msa0170000:intron | 35.0% | |
| ! | TGGGTTTGGGTTAATAAAGA+TGG | + | chr1_4:53562646-53562665 | Msa0170000:CDS | 35.0% |
| TAAAGATGGAATTGTCACTC+AGG | + | chr1_4:53562660-53562679 | Msa0170000:CDS | 35.0% | |
| GTCTGATGAATGCAAAGGTA+AGG | + | chr1_4:53562381-53562400 | Msa0170000:intron | 40.0% | |
| TAGCAGAAGCTACAATTCAG+AGG | + | chr1_4:53562548-53562567 | Msa0170000:CDS | 40.0% | |
| AGCAGAAGCTACAATTCAGA+GGG | + | chr1_4:53562549-53562568 | Msa0170000:CDS | 40.0% | |
| AATTCAGAGGGAGAATCCAT+TGG | + | chr1_4:53562561-53562580 | Msa0170000:CDS | 40.0% | |
| ACAATGATGGCATCAACCAA+TGG | - | chr1_4:53562580-53562599 | None:intergenic | 40.0% | |
| AGTTCCTTCTAGCACAATGA+TGG | - | chr1_4:53562593-53562612 | None:intergenic | 40.0% | |
| GTCACTCAGGTTCCAAAAAT+TGG | + | chr1_4:53562673-53562692 | Msa0170000:CDS | 40.0% | |
| TGTGCTCAGGTAAGAGTTCA+TGG | + | chr1_4:53562497-53562516 | Msa0170000:intron | 45.0% | |
| TAAGAGTTCATGGCCAGAGT+TGG | + | chr1_4:53562507-53562526 | Msa0170000:CDS | 45.0% | |
| GATGCCATCATTGTGCTAGA+AGG | + | chr1_4:53562586-53562605 | Msa0170000:CDS | 45.0% | |
| !!! | AAATTTTAATTATAATTTGA+TGG | + | chr1_4:53562452-53562471 | Msa0170000:intron | 5.0% |
| ! | AGTTCATGGCCAGAGTTGGT+TGG | + | chr1_4:53562511-53562530 | Msa0170000:CDS | 50.0% |
| GTTGGTTGGAGTGGAAGGAA+AGG | + | chr1_4:53562525-53562544 | Msa0170000:CDS | 50.0% | |
| TGTCACAGCGGAAGTCAAAG+GGG | - | chr1_4:53562621-53562640 | None:intergenic | 50.0% | |
| CTGTCACAGCGGAAGTCAAA+GGG | - | chr1_4:53562622-53562641 | None:intergenic | 50.0% | |
| ! | CTTTGACTTCCGCTGTGACA+GGG | + | chr1_4:53562621-53562640 | Msa0170000:CDS | 50.0% |
| AAACCCAAACCCTGTCACAG+CGG | - | chr1_4:53562633-53562652 | None:intergenic | 50.0% | |
| ! | CTGTGACAGGGTTTGGGTTT+GGG | + | chr1_4:53562633-53562652 | Msa0170000:CDS | 50.0% |
| ! | ATGGCCAGAGTTGGTTGGAG+TGG | + | chr1_4:53562516-53562535 | Msa0170000:CDS | 55.0% |
| CCTTCCACTCCAACCAACTC+TGG | - | chr1_4:53562523-53562542 | None:intergenic | 55.0% | |
| ! | CCAGAGTTGGTTGGAGTGGA+AGG | + | chr1_4:53562520-53562539 | Msa0170000:CDS | 55.0% |
| CCTGTCACAGCGGAAGTCAA+AGG | - | chr1_4:53562623-53562642 | None:intergenic | 55.0% | |
| ! | CCTTTGACTTCCGCTGTGAC+AGG | + | chr1_4:53562620-53562639 | Msa0170000:CDS | 55.0% |
| ACTTCCGCTGTGACAGGGTT+TGG | + | chr1_4:53562626-53562645 | Msa0170000:CDS | 55.0% | |
| CTTCCGCTGTGACAGGGTTT+GGG | + | chr1_4:53562627-53562646 | Msa0170000:CDS | 55.0% | |
| ! | GCTGTGACAGGGTTTGGGTT+TGG | + | chr1_4:53562632-53562651 | Msa0170000:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_4 | gene | 53562281 | 53562901 | 53562281 | ID=Msa0170000;Name=Msa0170000 |
| chr1_4 | mRNA | 53562281 | 53562901 | 53562281 | ID=Msa0170000-mRNA-1;Parent=Msa0170000;Name=Msa0170000-mRNA-1;_AED=0.01;_eAED=0.01;_QI=98|1|1|1|1|1|2|202|70 |
| chr1_4 | exon | 53562281 | 53562397 | 53562281 | ID=Msa0170000-mRNA-1:exon:15901;Parent=Msa0170000-mRNA-1 |
| chr1_4 | exon | 53562506 | 53562901 | 53562506 | ID=Msa0170000-mRNA-1:exon:15902;Parent=Msa0170000-mRNA-1 |
| chr1_4 | five_prime_UTR | 53562281 | 53562378 | 53562281 | ID=Msa0170000-mRNA-1:five_prime_utr;Parent=Msa0170000-mRNA-1 |
| chr1_4 | CDS | 53562379 | 53562397 | 53562379 | ID=Msa0170000-mRNA-1:cds;Parent=Msa0170000-mRNA-1 |
| chr1_4 | CDS | 53562506 | 53562699 | 53562506 | ID=Msa0170000-mRNA-1:cds;Parent=Msa0170000-mRNA-1 |
| chr1_4 | three_prime_UTR | 53562700 | 53562901 | 53562700 | ID=Msa0170000-mRNA-1:three_prime_utr;Parent=Msa0170000-mRNA-1 |
| Gene Sequence |
| Protein sequence |
