AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1437730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1437730 MtrunA17_Chr8g0382221 100.000 157 0 0 1 157 51 207 3.89e-116 327
Msa1437730 MtrunA17_Chr3g0123971 92.357 157 11 1 1 157 51 206 1.13e-104 298
Msa1437730 MtrunA17_Chr5g0400131 80.892 157 30 0 1 157 51 207 2.14e-94 271
Msa1437730 MtrunA17_Chr4g0055081 81.529 157 28 1 1 157 51 206 5.91e-94 270
Msa1437730 MtrunA17_Chr5g0433561 43.802 121 68 0 1 121 52 172 2.79e-36 124
Msa1437730 MtrunA17_Chr8g0349711 49.153 118 60 0 3 120 50 167 1.75e-35 122
Msa1437730 MtrunA17_Chr1g0199331 47.826 115 60 0 7 121 80 194 4.94e-35 120
Msa1437730 MtrunA17_Chr3g0109721 42.975 121 69 0 1 121 52 172 5.19e-35 120
Msa1437730 MtrunA17_Chr8g0390561 46.218 119 64 0 3 121 53 171 3.88e-34 118
Msa1437730 MtrunA17_Chr2g0290891 42.373 118 68 0 3 120 50 167 9.31e-33 115
Msa1437730 MtrunA17_Chr8g0349681 47.458 118 62 0 3 120 219 336 1.55e-32 121
Msa1437730 MtrunA17_Chr8g0349681 46.610 118 63 0 3 120 428 545 2.94e-31 117
Msa1437730 MtrunA17_Chr8g0349681 45.378 119 65 0 3 121 57 175 3.38e-31 117
Msa1437730 MtrunA17_Chr6g0458081 42.500 120 69 0 1 120 48 167 2.89e-32 114
Msa1437730 MtrunA17_Chr4g0032201 35.758 165 93 3 1 156 54 214 2.63e-31 112
Msa1437730 MtrunA17_Chr7g0250531 38.312 154 85 2 6 156 55 201 2.47e-30 108
Msa1437730 MtrunA17_Chr4g0055651 37.086 151 91 1 6 156 55 201 1.27e-29 107
Msa1437730 MtrunA17_Chr2g0276931 37.162 148 90 1 3 147 53 200 4.12e-29 105
Msa1437730 MtrunA17_Chr8g0335471 37.419 155 86 3 6 156 55 202 6.54e-29 105
Msa1437730 MtrunA17_Chr1g0212521 36.538 156 95 3 3 155 59 213 8.73e-29 105
Msa1437730 MtrunA17_Chr8g0346421 43.243 111 63 0 6 116 73 183 1.23e-28 105
Msa1437730 MtrunA17_Chr2g0276941 40.351 114 68 0 1 114 57 170 1.41e-28 105
Msa1437730 MtrunA17_Chr4g0030911 35.669 157 95 3 3 155 59 213 1.79e-28 104
Msa1437730 MtrunA17_Chr8g0351861 37.288 118 74 0 3 120 60 177 1.98e-28 104
Msa1437730 MtrunA17_Chr3g0112681 33.742 163 96 2 3 156 56 215 2.27e-28 104
Msa1437730 MtrunA17_Chr1g0182321 43.103 116 65 1 3 117 59 174 2.50e-28 104
Msa1437730 MtrunA17_Chr6g0482721 39.655 116 70 0 5 120 58 173 2.76e-28 103
Msa1437730 MtrunA17_Chr3g0123821 33.121 157 89 2 1 157 58 198 3.42e-28 103
Msa1437730 MtrunA17_Chr1g0182301 43.103 116 65 1 3 117 59 174 3.88e-28 103
Msa1437730 MtrunA17_Chr7g0263071 43.103 116 65 1 3 117 59 174 4.01e-28 103
Msa1437730 MtrunA17_Chr8g0381841 37.931 116 72 0 1 116 59 174 7.03e-28 103
Msa1437730 MtrunA17_Chr7g0214241 35.625 160 98 2 3 157 59 218 7.77e-28 103
Msa1437730 MtrunA17_Chr7g0234201 35.849 159 97 2 3 157 57 214 2.37e-27 101
Msa1437730 MtrunA17_Chr1g0180291 39.355 155 85 4 6 155 60 210 2.92e-27 101
Msa1437730 MtrunA17_Chr7g0275991 39.640 111 67 0 6 116 67 177 4.83e-27 101
Msa1437730 MtrunA17_Chr7g0231131 32.237 152 89 1 1 152 57 194 7.37e-27 100
Msa1437730 MtrunA17_Chr4g0046261 37.069 116 73 0 1 116 55 170 1.04e-26 100
Msa1437730 MtrunA17_Chr1g0174281 30.723 166 104 1 1 155 57 222 1.08e-26 100
Msa1437730 MtrunA17_Chr1g0189361 38.793 116 71 0 1 116 56 171 1.73e-26 99.8
Msa1437730 MtrunA17_Chr1g0197331 39.610 154 88 4 5 156 62 212 2.95e-26 98.6
Msa1437730 MtrunA17_Chr2g0327911 41.026 117 67 2 3 117 59 175 5.68e-26 98.2
Msa1437730 MtrunA17_Chr4g0040781 37.069 116 73 0 1 116 55 170 6.49e-26 98.2
Msa1437730 MtrunA17_Chr2g0283131 37.719 114 71 0 3 116 57 170 7.14e-26 97.8
Msa1437730 MtrunA17_Chr2g0315551 35.965 114 73 0 3 116 57 170 1.36e-25 97.1
Msa1437730 MtrunA17_Chr7g0265001 36.306 157 89 4 6 156 63 214 9.75e-25 94.7
Msa1437730 MtrunA17_Chr2g0325091 35.659 129 83 0 5 133 57 185 9.97e-25 94.7
Msa1437730 MtrunA17_Chr2g0319951 33.333 120 80 0 1 120 55 174 5.85e-24 92.8
Msa1437730 MtrunA17_Chr8g0392931 34.228 149 92 2 3 150 58 201 1.68e-23 91.7
Msa1437730 MtrunA17_Chr8g0384311 39.655 116 68 2 3 116 56 171 3.61e-23 90.5
Msa1437730 MtrunA17_Chr4g0034871 36.810 163 87 6 3 157 52 206 1.54e-22 89.0
Msa1437730 MtrunA17_Chr4g0004661 37.037 162 89 4 3 157 108 263 3.08e-22 89.4
Msa1437730 MtrunA17_Chr3g0130271 40.171 117 67 3 3 116 55 171 5.63e-22 87.4
Msa1437730 MtrunA17_Chr2g0331691 35.802 162 91 4 3 157 54 209 5.57e-21 85.1
Msa1437730 MtrunA17_Chr1g0159481 37.069 116 71 2 3 116 60 175 1.07e-20 84.3
Msa1437730 MtrunA17_Chr6g0466301 33.929 168 89 6 1 157 50 206 4.86e-20 82.4
Msa1437730 MtrunA17_Chr6g0465491 33.929 168 89 6 1 157 50 206 4.86e-20 82.4
Msa1437730 MtrunA17_Chr1g0201121 32.317 164 98 4 1 157 50 207 1.24e-19 81.3
Msa1437730 MtrunA17_Chr8g0383261 30.488 164 99 5 1 157 54 209 1.79e-19 80.9
Msa1437730 MtrunA17_Chr3g0117931 37.398 123 64 4 3 113 51 172 4.17e-15 69.7
Msa1437730 MtrunA17_Chr6g0466161 27.439 164 104 4 1 157 32 187 1.95e-14 67.8
Msa1437730 MtrunA17_Chr3g0138121 33.333 120 77 3 8 127 62 178 5.81e-14 66.6
Msa1437730 MtrunA17_Chr3g0138101 33.333 120 77 3 8 127 62 178 5.87e-14 66.6
Msa1437730 MtrunA17_Chr2g0325101 34.959 123 67 4 3 113 49 170 5.92e-14 66.2
Msa1437730 MtrunA17_Chr3g0138111 33.333 120 77 3 8 127 62 178 6.37e-14 66.6
Msa1437730 MtrunA17_Chr6g0465471 26.829 164 105 4 1 157 32 187 1.05e-13 65.9
Msa1437730 MtrunA17_Chr6g0486041 36.441 118 62 4 8 113 54 170 6.09e-13 63.5
Msa1437730 MtrunA17_Chr4g0046211 37.500 88 53 2 3 89 49 135 5.01e-12 60.1
Msa1437730 MtrunA17_Chr4g0037021 31.707 123 71 4 3 113 49 170 6.48e-12 60.8
Msa1437730 MtrunA17_Chr8g0371501 31.933 119 68 4 7 113 53 170 2.03e-11 59.3
Msa1437730 MtrunA17_Chr5g0405791 32.203 118 67 4 8 113 54 170 2.44e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1437730 AT2G22290.1 84.713 157 24 0 1 157 51 207 3.61e-99 284
Msa1437730 AT5G10260.1 84.713 157 24 0 1 157 51 207 3.86e-99 284
Msa1437730 AT2G44610.1 82.278 158 27 1 1 157 51 208 3.52e-95 274
Msa1437730 AT5G64990.1 75.484 155 37 1 4 157 52 206 1.72e-81 239
Msa1437730 AT4G39890.1 73.780 164 36 3 1 157 51 214 6.60e-80 236
Msa1437730 AT5G64990.2 72.222 162 37 2 4 157 52 213 1.23e-78 232
Msa1437730 AT4G19640.1 42.975 121 69 0 1 121 52 172 2.15e-35 122
Msa1437730 AT5G45130.1 42.975 121 69 0 1 121 52 172 3.36e-35 121
Msa1437730 AT5G45130.2 42.975 121 69 0 1 121 60 180 1.17e-34 120
Msa1437730 AT3G54840.3 47.826 115 60 0 7 121 48 162 2.42e-34 118
Msa1437730 AT3G54840.1 47.826 115 60 0 7 121 82 196 4.35e-34 119
Msa1437730 AT4G17170.1 36.250 160 95 2 3 155 50 209 6.13e-34 119
Msa1437730 AT4G35860.2 36.875 160 94 3 3 155 4 163 6.63e-33 114
Msa1437730 AT4G35860.1 36.875 160 94 3 3 155 50 209 3.21e-32 114
Msa1437730 AT3G54840.2 48.598 107 55 0 7 113 82 188 5.23e-32 113
Msa1437730 AT4G17160.1 40.678 118 70 0 3 120 50 167 3.71e-31 111
Msa1437730 AT5G03520.2 37.821 156 95 2 3 156 49 204 5.20e-31 111
Msa1437730 AT5G03520.1 37.821 156 95 2 3 156 59 214 5.43e-31 111
Msa1437730 AT3G11730.1 38.462 156 86 3 4 155 53 202 1.59e-30 110
Msa1437730 AT1G09630.1 41.525 118 69 0 1 118 54 171 2.40e-30 109
Msa1437730 AT1G05810.1 37.267 161 97 2 1 157 54 214 7.52e-30 108
Msa1437730 AT2G31680.1 37.654 162 96 2 1 157 54 215 1.12e-29 108
Msa1437730 AT1G07410.1 40.000 120 72 0 1 120 54 173 2.04e-29 107
Msa1437730 AT1G05810.2 37.267 161 97 2 1 157 100 260 2.07e-29 108
Msa1437730 AT1G02130.1 42.609 115 66 0 6 120 55 169 2.59e-29 106
Msa1437730 AT3G46830.1 39.474 114 69 0 3 116 56 169 1.52e-28 105
Msa1437730 AT3G46830.2 39.474 114 69 0 3 116 56 169 1.52e-28 105
Msa1437730 AT3G53610.2 37.419 155 88 3 3 156 59 205 1.68e-28 105
Msa1437730 AT3G53610.3 37.419 155 88 3 3 156 59 205 1.68e-28 105
Msa1437730 AT3G53610.1 37.419 155 88 3 3 156 59 205 1.68e-28 105
Msa1437730 AT5G59150.2 39.474 114 69 0 3 116 5 118 1.70e-28 103
Msa1437730 AT4G17530.1 36.129 155 91 2 4 156 53 201 2.27e-28 104
Msa1437730 AT5G59840.1 38.312 154 85 4 3 154 59 204 2.70e-28 104
Msa1437730 AT3G46060.2 38.312 154 85 4 3 154 59 204 3.61e-28 104
Msa1437730 AT3G46060.3 38.312 154 85 4 3 154 59 204 3.61e-28 104
Msa1437730 AT3G46060.1 38.312 154 85 4 3 154 59 204 3.61e-28 104
Msa1437730 AT5G47200.1 41.026 117 69 0 4 120 53 169 3.76e-28 103
Msa1437730 AT3G09900.1 37.888 161 90 3 3 156 59 216 3.86e-28 103
Msa1437730 AT5G47520.1 35.152 165 98 4 1 156 56 220 3.92e-28 104
Msa1437730 AT5G65270.1 36.207 116 74 0 1 116 59 174 5.81e-28 103
Msa1437730 AT5G59150.1 38.793 116 71 0 1 116 54 169 6.11e-28 103
Msa1437730 AT1G18200.1 43.119 109 62 0 5 113 59 167 9.44e-28 103
Msa1437730 AT5G47960.1 33.540 161 99 2 3 155 59 219 3.61e-27 101
Msa1437730 AT2G43130.1 35.220 159 98 2 1 155 54 211 4.01e-27 101
Msa1437730 AT1G73640.1 39.823 113 68 0 1 113 55 167 5.34e-27 101
Msa1437730 AT5G60860.1 32.895 152 84 1 1 134 55 206 8.52e-27 100
Msa1437730 AT3G12160.1 35.802 162 97 3 1 155 57 218 9.43e-27 100
Msa1437730 AT4G39990.1 34.167 120 79 0 1 120 59 178 1.60e-26 100
Msa1437730 AT3G15060.1 36.667 120 76 0 1 120 55 174 2.18e-26 99.8
Msa1437730 AT4G18430.1 32.258 155 87 1 1 137 55 209 4.33e-26 99.0
Msa1437730 AT1G28550.1 38.938 113 69 0 4 116 58 170 4.98e-26 98.6
Msa1437730 AT3G07410.1 42.342 111 64 0 3 113 56 166 2.08e-25 97.1
Msa1437730 AT3G09910.3 38.608 158 88 5 1 156 54 204 5.92e-25 95.5
Msa1437730 AT3G09910.1 38.608 158 88 5 1 156 54 204 5.92e-25 95.5
Msa1437730 AT3G09910.2 38.608 158 88 5 1 156 54 204 5.92e-25 95.5
Msa1437730 AT1G16920.1 33.333 120 80 0 1 120 55 174 1.11e-24 95.1
Msa1437730 AT2G33870.1 36.441 118 75 0 3 120 57 174 1.39e-24 95.1
Msa1437730 AT4G19640.2 43.023 86 49 0 1 86 52 137 2.14e-24 92.4
Msa1437730 AT5G45750.1 31.325 166 95 5 3 156 57 215 2.22e-24 94.4
Msa1437730 AT5G03530.2 38.217 157 86 6 6 156 59 210 2.86e-24 94.0
Msa1437730 AT5G03530.1 38.217 157 86 6 6 156 59 210 2.86e-24 94.0
Msa1437730 AT3G09910.5 41.176 119 68 2 1 117 54 172 4.01e-24 93.2
Msa1437730 AT3G09910.4 41.176 119 68 2 1 117 54 172 4.01e-24 93.2
Msa1437730 AT1G01200.1 35.329 167 95 5 3 157 72 237 7.01e-24 93.6
Msa1437730 AT4G19640.3 41.237 97 53 1 1 97 52 144 8.09e-24 92.4
Msa1437730 AT1G43890.3 40.157 127 72 3 5 129 58 182 3.51e-23 91.3
Msa1437730 AT1G43890.1 40.157 127 72 3 5 129 58 182 3.51e-23 91.3
Msa1437730 AT1G43890.2 40.157 127 72 3 5 129 58 182 3.51e-23 91.3
Msa1437730 AT1G06400.1 35.088 114 74 0 3 116 57 170 4.25e-23 90.9
Msa1437730 AT3G18820.1 37.423 163 86 6 3 157 52 206 5.31e-23 90.5
Msa1437730 AT4G18800.1 34.167 120 75 2 3 120 57 174 1.62e-22 89.4
Msa1437730 AT1G52280.1 36.420 162 89 4 3 157 52 206 5.53e-22 87.8
Msa1437730 AT3G16100.1 36.420 162 89 4 3 157 52 206 4.43e-20 82.8
Msa1437730 AT4G09720.6 34.146 164 94 6 1 157 16 172 5.19e-20 82.0
Msa1437730 AT4G09720.5 34.146 164 94 6 1 157 16 172 5.19e-20 82.0
Msa1437730 AT4G09720.2 34.146 164 94 6 1 157 16 172 5.19e-20 82.0
Msa1437730 AT4G09720.1 34.146 164 94 6 1 157 50 206 1.42e-19 81.6
Msa1437730 AT1G22740.1 32.927 164 93 6 1 157 50 203 1.46e-19 81.6
Msa1437730 AT5G39620.1 34.356 163 92 5 3 157 49 204 1.76e-19 81.3
Msa1437730 AT2G21880.4 33.918 171 90 6 1 157 26 187 2.82e-19 80.5
Msa1437730 AT2G21880.3 33.918 171 90 6 1 157 26 187 2.82e-19 80.5
Msa1437730 AT1G49300.2 34.969 163 90 6 3 157 52 206 3.24e-19 80.5
Msa1437730 AT1G49300.1 34.969 163 90 6 3 157 52 206 3.24e-19 80.5
Msa1437730 AT2G21880.1 33.918 171 90 6 1 157 51 212 4.78e-19 80.5
Msa1437730 AT4G09720.4 32.544 169 95 6 1 157 50 211 1.07e-17 76.6
Msa1437730 AT4G09720.3 31.429 175 95 6 1 157 50 217 4.37e-17 75.1
Msa1437730 AT2G44690.1 37.398 123 64 4 3 113 61 182 3.81e-16 72.8
Msa1437730 AT5G55080.1 32.500 120 78 3 8 127 62 178 1.05e-15 71.6
Msa1437730 AT5G20010.1 31.250 144 92 4 8 151 62 198 6.56e-15 69.7
Msa1437730 AT5G46025.1 36.782 87 54 1 35 120 20 106 7.85e-15 67.0
Msa1437730 AT5G20020.1 31.250 144 92 4 8 151 62 198 1.02e-14 68.9
Msa1437730 AT3G48040.1 37.398 123 64 4 3 113 51 172 1.85e-14 68.2
Msa1437730 AT5G55190.1 33.333 120 77 3 8 127 62 178 1.20e-13 66.2
Msa1437730 AT4G35020.1 34.146 123 68 4 3 113 49 170 1.59e-13 65.5
Msa1437730 AT4G35020.2 34.146 123 68 4 3 113 49 170 1.59e-13 65.5
Msa1437730 AT4G35020.3 34.146 123 68 4 3 113 49 170 1.59e-13 65.5
Msa1437730 AT5G62880.1 35.772 123 66 4 3 113 51 172 3.61e-13 64.7
Msa1437730 AT3G48040.2 37.719 114 58 4 3 104 51 163 8.42e-13 63.2
Msa1437730 AT1G20090.1 33.333 123 69 4 3 113 48 169 9.33e-13 63.5
Msa1437730 AT3G51300.1 34.454 119 65 4 7 113 53 170 1.14e-12 63.2
Msa1437730 AT1G75840.1 33.333 123 69 4 3 113 49 170 1.18e-12 63.2
Msa1437730 AT3G51290.2 34.454 119 65 4 7 113 654 771 2.47e-12 63.9
Msa1437730 AT4G35950.1 34.454 119 65 4 7 113 53 170 4.09e-12 61.6
Msa1437730 AT2G17800.2 34.454 119 65 4 7 113 53 170 5.35e-12 61.2
Msa1437730 AT2G17800.1 34.454 119 65 4 7 113 53 170 5.35e-12 61.2
Msa1437730 AT5G45970.1 30.894 123 72 4 3 113 49 170 1.46e-11 60.1
Msa1437730 AT5G62880.2 35.965 114 60 4 3 104 51 163 1.78e-11 59.7
Msa1437730 AT4G28950.1 32.231 121 71 4 3 113 49 168 2.39e-11 59.7

Find 63 sgRNAs with CRISPR-Local

Find 137 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAATGATCTTCAGTTAAT+TGG 0.179000 tig0028361:-1496 Msa1437730:three_prime_UTR
TAAGAAAACGAGCTTGTATT+TGG 0.223259 tig0028361:+3775 None:intergenic
AACCCTTTCTTCTACAAATA+AGG 0.224029 tig0028361:-1702 Msa1437730:CDS
TGAGACTTCTAAATCTTTCT+TGG 0.265565 tig0028361:+2794 None:intergenic
CGATCCATTTCTCTCATAAA+TGG 0.286173 tig0028361:-3816 Msa1437730:five_prime_UTR
AGTCGAAAATGGCGTGTATT+TGG 0.316103 tig0028361:-3966 None:intergenic
GAACTGTTCGTTTGCAGCTT+TGG 0.333518 tig0028361:-2977 Msa1437730:intron
TCCTCCCTGCTGCTCTGTAT+TGG 0.354070 tig0028361:+1637 None:intergenic
CTTCCTTATTTGTAGAAGAA+AGG 0.383495 tig0028361:+1699 None:intergenic
GAGGAGGTTGCTCATGTTAG+AGG 0.391358 tig0028361:-1619 Msa1437730:exon
AAGATTGCTTCCGCCTTGCC+AGG 0.393556 tig0028361:-1731 Msa1437730:CDS
GATGCCAAGTCAAAAGAGTT+TGG 0.394493 tig0028361:-2074 Msa1437730:CDS
AACGAACAGTTCTATCTTCA+AGG 0.399142 tig0028361:+2988 None:intergenic
CATGAAGCGGGTGATGATAC+TGG 0.410880 tig0028361:+3730 None:intergenic
GAAGAAAGGGTTTCCATCCC+TGG 0.417515 tig0028361:+1713 None:intergenic
ATTATTGTATTCTTACATCC+AGG 0.418547 tig0028361:+1390 None:intergenic
CAAATTCGATACCACCTATC+AGG 0.420018 tig0028361:-3702 Msa1437730:intron
TTCCTTATTTGTAGAAGAAA+GGG 0.422408 tig0028361:+1700 None:intergenic
GAAGAATCTCTTATGTAGCT+TGG 0.431932 tig0028361:+2769 None:intergenic
TGCTTCCGCCTTGCCAGGGA+TGG 0.448090 tig0028361:-1726 Msa1437730:CDS
GTGGGGACAAATTGCGGTAA+TGG 0.449056 tig0028361:-1470 Msa1437730:three_prime_UTR
TTCATCCAATACAGAGCAGC+AGG 0.451660 tig0028361:-1642 Msa1437730:CDS
GTCGCCATTTATGAGAGAAA+TGG 0.453134 tig0028361:+3812 None:intergenic
TCTTAAATGTAAAGCAAAGC+TGG 0.459400 tig0028361:-1536 Msa1437730:three_prime_UTR
AAACTGATCTTGTCGAGAAA+AGG 0.473751 tig0028361:-2440 Msa1437730:intron
TTCTTAGGCGATCAATCCGT+TGG 0.478079 tig0028361:-3758 Msa1437730:five_prime_UTR
AGGCAAGTTTCTATAGAAGA+AGG 0.486341 tig0028361:-2098 Msa1437730:CDS
GATGTTATAATTGTCTTGGT+TGG 0.487954 tig0028361:-2468 Msa1437730:CDS
TAGTGATGTTATAATTGTCT+TGG 0.496039 tig0028361:-2472 Msa1437730:CDS
ATCGAAACCAGTGCAAAAGC+AGG 0.513342 tig0028361:-2041 Msa1437730:CDS
TTTGTTATACAATTAGTCCC+TGG 0.518774 tig0028361:-1408 Msa1437730:three_prime_UTR
AATGAAGAATTGAACCTGAT+AGG 0.522083 tig0028361:+3688 None:intergenic
AATACAGAGCAGCAGGGAGG+AGG 0.528419 tig0028361:-1635 Msa1437730:CDS
GTATTGGATGAATGCACTGT+GGG 0.529684 tig0028361:+1653 None:intergenic
TTAACTTTCAGGGATACTGC+AGG 0.536162 tig0028361:-2817 Msa1437730:intron
ATATAATTTCAGGCTACTAT+CGG 0.539656 tig0028361:-3037 Msa1437730:intron
TTATTGTATTCTTACATCCA+GGG 0.549799 tig0028361:+1391 None:intergenic
AAAAGCAGGCTTCAATATCA+AGG 0.552081 tig0028361:-2027 Msa1437730:intron
CACTAACAAGTGGGTTGAGG+AGG 0.553351 tig0028361:-2511 Msa1437730:CDS
GAACACTAACAAGTGGGTTG+AGG 0.553502 tig0028361:-2514 Msa1437730:CDS
GAATTTGTCGTACATGAAGC+GGG 0.561469 tig0028361:+3718 None:intergenic
TTCTACAAATAAGGAAGACA+TGG 0.563578 tig0028361:-1693 Msa1437730:CDS
AGATTGCTTCCGCCTTGCCA+GGG 0.573903 tig0028361:-1730 Msa1437730:CDS
CATTTATGAGAGAAATGGAT+CGG 0.579731 tig0028361:+3817 None:intergenic
TCTTCAGTTAATTGGTGTGT+GGG 0.579969 tig0028361:-1488 Msa1437730:three_prime_UTR
CGAATTTGTCGTACATGAAG+CGG 0.587508 tig0028361:+3717 None:intergenic
AAGGGTTTCCATCCCTGGCA+AGG 0.588850 tig0028361:+1718 None:intergenic
CATTTCTGAACACTAACAAG+TGG 0.594464 tig0028361:-2521 Msa1437730:CDS
ATCTTCAGTTAATTGGTGTG+TGG 0.604711 tig0028361:-1489 Msa1437730:three_prime_UTR
ATTTCTCTCATAAATGGCGA+CGG 0.612804 tig0028361:-3810 Msa1437730:five_prime_UTR
ATTTCTGAACACTAACAAGT+GGG 0.616106 tig0028361:-2520 Msa1437730:CDS
GAAGCAATCTTACGAAACAA+AGG 0.623923 tig0028361:+1743 None:intergenic
ACGAGCTTGTATTTGGCGAG+AGG 0.624355 tig0028361:+3782 None:intergenic
TTTCACTTGAGTATAATACT+GGG 0.651284 tig0028361:-1566 Msa1437730:three_prime_UTR
CTTCAGTTAATTGGTGTGTG+GGG 0.656528 tig0028361:-1487 Msa1437730:three_prime_UTR
TGGTGTGTGGGGACAAATTG+CGG 0.656876 tig0028361:-1476 Msa1437730:three_prime_UTR
GAGGAGGTGCGTCAAGAACG+TGG 0.657614 tig0028361:-2495 Msa1437730:CDS
TGTATTGGATGAATGCACTG+TGG 0.660739 tig0028361:+1652 None:intergenic
GAAGAATTGAACCTGATAGG+TGG 0.666711 tig0028361:+3691 None:intergenic
TAAATGTAAAGCAAAGCTGG+AGG 0.681704 tig0028361:-1533 Msa1437730:three_prime_UTR
TCCAATACAGAGCAGCAGGG+AGG 0.703010 tig0028361:-1638 Msa1437730:CDS
GGTTTCCATCCCTGGCAAGG+CGG 0.703532 tig0028361:+1721 None:intergenic
TCATCCAATACAGAGCAGCA+GGG 0.714266 tig0028361:-1641 Msa1437730:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAAATGTGATTTATTAA+GGG + tig0028361:1891-1910 None:intergenic 10.0%
!!! TTAATAAATCACATTTTTAA+GGG - tig0028361:1891-1910 Msa1437730:intron 10.0%
!! TATAACAAATGAAAAAATAA+CGG + tig0028361:3911-3930 None:intergenic 10.0%
!! CTTAAAAATGTGATTTATTA+AGG + tig0028361:1892-1911 None:intergenic 15.0%
!!! CTTAATAAATCACATTTTTA+AGG - tig0028361:1890-1909 Msa1437730:intron 15.0%
!!! ACAATGAATTTTAAAAAAAC+TGG + tig0028361:1945-1964 None:intergenic 15.0%
!! AAATTTGATCATATAATTTC+AGG - tig0028361:2283-2302 Msa1437730:intron 15.0%
!! TATCGTTATTTAACTAAAAA+AGG + tig0028361:2729-2748 None:intergenic 15.0%
!!! AGTTTTGTTTAATATCATAT+TGG - tig0028361:2981-3000 Msa1437730:CDS 15.0%
!! GTTAATGAAATTAATTGAAT+TGG + tig0028361:3142-3161 None:intergenic 15.0%
!! TCAATTAATTTCATTAACAA+AGG - tig0028361:3144-3163 Msa1437730:intron 15.0%
!!! TGGTCTAATTATTTTTTATA+TGG - tig0028361:3196-3215 Msa1437730:intron 15.0%
!! AGATTGATCATTCAAAAATT+AGG - tig0028361:1966-1985 Msa1437730:intron 20.0%
!!! AATTCTGATGATTTTTTAAG+AGG - tig0028361:2086-2105 Msa1437730:CDS 20.0%
!!! GCTTTTATGTTAGAATATTA+TGG - tig0028361:2108-2127 Msa1437730:intron 20.0%
!! TTCCAACATCATATAATAAA+TGG - tig0028361:2189-2208 Msa1437730:intron 20.0%
!! CAAAAAAATATAAATTGACG+AGG + tig0028361:2390-2409 None:intergenic 20.0%
!! TCAGAAATAGTAAAAAGAAA+AGG + tig0028361:2481-2500 None:intergenic 20.0%
!!! TTAGTGTATTTTAACTTTCA+GGG - tig0028361:2503-2522 Msa1437730:CDS 20.0%
!! AGGTTAATAACTATAAATTG+AGG + tig0028361:2759-2778 None:intergenic 20.0%
!! ATGAATATATAGATCAAACT+CGG + tig0028361:2930-2949 None:intergenic 20.0%
!! AATTAATTGAATTGGCTATA+AGG + tig0028361:3134-3153 None:intergenic 20.0%
!! TATTCGATGTAAAACAAAAT+AGG + tig0028361:3474-3493 None:intergenic 20.0%
!! TTCTCAATTTCAATTTTGCA+TGG - tig0028361:1671-1690 Msa1437730:CDS 25.0%
! CCAATCACATAAAAATCATA+GGG + tig0028361:1783-1802 None:intergenic 25.0%
! TCCAATCACATAAAAATCAT+AGG + tig0028361:1784-1803 None:intergenic 25.0%
! CTCCATTTATTATATGATGT+TGG + tig0028361:2194-2213 None:intergenic 25.0%
! CAACATCATATAATAAATGG+AGG - tig0028361:2192-2211 Msa1437730:intron 25.0%
! ATATAATTTCAGGCTACTAT+CGG - tig0028361:2293-2312 Msa1437730:intron 25.0%
! AGATGATGCATTATGATTTA+TGG - tig0028361:2432-2451 Msa1437730:intron 25.0%
!!! CTTAGTGTATTTTAACTTTC+AGG - tig0028361:2502-2521 Msa1437730:CDS 25.0%
! TAGTGATGTTATAATTGTCT+TGG - tig0028361:2858-2877 Msa1437730:intron 25.0%
! TCTATATATTCATTCACAAC+CGG - tig0028361:2937-2956 Msa1437730:intron 25.0%
! TAGCAAAATTACAAAGAACA+TGG + tig0028361:3082-3101 None:intergenic 25.0%
!!! CTTTGTAATTTTGCTATGAT+AGG - tig0028361:3086-3105 Msa1437730:intron 25.0%
! TTTCAAATATTGCGATGTTA+TGG - tig0028361:3176-3195 Msa1437730:intron 25.0%
!! AATGAATTTTCATAGTTGCT+TGG - tig0028361:3334-3353 Msa1437730:intron 25.0%
!! ATGAATTTTCATAGTTGCTT+GGG - tig0028361:3335-3354 Msa1437730:intron 25.0%
! TTAGATGTGAAAGTTAGTAA+AGG + tig0028361:3442-3461 None:intergenic 25.0%
!! TTCCTTATTTGTAGAAGAAA+GGG + tig0028361:3633-3652 None:intergenic 25.0%
!! TTTTCACTTGAGTATAATAC+TGG - tig0028361:3763-3782 Msa1437730:five_prime_UTR 25.0%
! TTTCACTTGAGTATAATACT+GGG - tig0028361:3764-3783 Msa1437730:five_prime_UTR 25.0%
! ATCAATGATCTTCAGTTAAT+TGG - tig0028361:3834-3853 Msa1437730:five_prime_UTR 25.0%
! TTATTGTATTCTTACATCCA+GGG + tig0028361:3942-3961 None:intergenic 25.0%
! ATTATTGTATTCTTACATCC+AGG + tig0028361:3943-3962 None:intergenic 25.0%
CATTTATGAGAGAAATGGAT+CGG + tig0028361:1516-1535 None:intergenic 30.0%
TAAGAAAACGAGCTTGTATT+TGG + tig0028361:1558-1577 None:intergenic 30.0%
! AAATACAAGCTCGTTTTCTT+AGG - tig0028361:1557-1576 Msa1437730:three_prime_UTR 30.0%
AATGAAGAATTGAACCTGAT+AGG + tig0028361:1645-1664 None:intergenic 30.0%
! CCCTATGATTTTTATGTGAT+TGG - tig0028361:1780-1799 Msa1437730:intron 30.0%
!! TGATCACAAGTTGATGAATT+AGG - tig0028361:1827-1846 Msa1437730:intron 30.0%
!! AAGTTGATGAATTAGGACTT+TGG - tig0028361:1834-1853 Msa1437730:intron 30.0%
! TCATTCATAAGTCACTTTCA+AGG - tig0028361:2050-2069 Msa1437730:CDS 30.0%
TGAAACAAAAAACACCTACA+CGG + tig0028361:2458-2477 None:intergenic 30.0%
TGAGACTTCTAAATCTTTCT+TGG + tig0028361:2539-2558 None:intergenic 30.0%
TATGTGTTTGCATTTGAATC+TGG - tig0028361:2679-2698 Msa1437730:intron 30.0%
TACATTAGAACAGCTTACTT+AGG + tig0028361:2779-2798 None:intergenic 30.0%
ATTTCTGAACACTAACAAGT+GGG - tig0028361:2810-2829 Msa1437730:intron 30.0%
GATGTTATAATTGTCTTGGT+TGG - tig0028361:2862-2881 Msa1437730:intron 30.0%
!!! TTATTTTTTATATGGCCGCT+TGG - tig0028361:3204-3223 Msa1437730:intron 30.0%
AACCCTTTCTTCTACAAATA+AGG - tig0028361:3628-3647 Msa1437730:intron 30.0%
! CTTCCTTATTTGTAGAAGAA+AGG + tig0028361:3634-3653 None:intergenic 30.0%
TTCTACAAATAAGGAAGACA+TGG - tig0028361:3637-3656 Msa1437730:intron 30.0%
TCTTAAATGTAAAGCAAAGC+TGG - tig0028361:3794-3813 Msa1437730:five_prime_UTR 30.0%
! TTTGTTATACAATTAGTCCC+TGG - tig0028361:3922-3941 Msa1437730:five_prime_UTR 30.0%
CGATCCATTTCTCTCATAAA+TGG - tig0028361:1514-1533 Msa1437730:three_prime_UTR 35.0%
ATTTCTCTCATAAATGGCGA+CGG - tig0028361:1520-1539 Msa1437730:three_prime_UTR 35.0%
AATTAGGACTTTGGACGATT+AGG - tig0028361:1843-1862 Msa1437730:intron 35.0%
GGTTTCAACGACTCAAATTA+TGG + tig0028361:1994-2013 None:intergenic 35.0%
TAGCAATCTAGCAACATAGA+GGG + tig0028361:2258-2277 None:intergenic 35.0%
TTAGCAATCTAGCAACATAG+AGG + tig0028361:2259-2278 None:intergenic 35.0%
AACGAACAGTTCTATCTTCA+AGG + tig0028361:2345-2364 None:intergenic 35.0%
ATTATGATTTATGGCCGTGT+AGG - tig0028361:2441-2460 Msa1437730:CDS 35.0%
GAAGAATCTCTTATGTAGCT+TGG + tig0028361:2564-2583 None:intergenic 35.0%
TAAGCTGTTCTAATGTAGAC+AGG - tig0028361:2782-2801 Msa1437730:CDS 35.0%
CATTTCTGAACACTAACAAG+TGG - tig0028361:2809-2828 Msa1437730:CDS 35.0%
AAACTGATCTTGTCGAGAAA+AGG - tig0028361:2890-2909 Msa1437730:intron 35.0%
AGGCAAGTTTCTATAGAAGA+AGG - tig0028361:3232-3251 Msa1437730:intron 35.0%
!! GATTCCAAACTCTTTTGACT+TGG + tig0028361:3263-3282 None:intergenic 35.0%
AAAAGCAGGCTTCAATATCA+AGG - tig0028361:3303-3322 Msa1437730:intron 35.0%
GTTTATCTTCTTTCCCATGT+TGG - tig0028361:3523-3542 Msa1437730:intron 35.0%
! TGCATTGACAGATAACAATG+AGG + tig0028361:3558-3577 None:intergenic 35.0%
GAAGCAATCTTACGAAACAA+AGG + tig0028361:3590-3609 None:intergenic 35.0%
TAAATGTAAAGCAAAGCTGG+AGG - tig0028361:3797-3816 Msa1437730:five_prime_UTR 35.0%
ATCTTCAGTTAATTGGTGTG+TGG - tig0028361:3841-3860 Msa1437730:five_prime_UTR 35.0%
TCTTCAGTTAATTGGTGTGT+GGG - tig0028361:3842-3861 Msa1437730:five_prime_UTR 35.0%
GTCGCCATTTATGAGAGAAA+TGG + tig0028361:1521-1540 None:intergenic 40.0%
!!! GATGATACTGGTTTTGCCAA+CGG + tig0028361:1591-1610 None:intergenic 40.0%
GAATTTGTCGTACATGAAGC+GGG + tig0028361:1615-1634 None:intergenic 40.0%
CGAATTTGTCGTACATGAAG+CGG + tig0028361:1616-1635 None:intergenic 40.0%
CAAATTCGATACCACCTATC+AGG - tig0028361:1628-1647 Msa1437730:CDS 40.0%
GAAGAATTGAACCTGATAGG+TGG + tig0028361:1642-1661 None:intergenic 40.0%
AGTCGTTGAAACCCTTGAAT+GGG - tig0028361:2001-2020 Msa1437730:intron 40.0%
GAACTCTTCTTCCCATTCAA+GGG + tig0028361:2015-2034 None:intergenic 40.0%
ATGGTGCAATTTCTGCTTAG+TGG - tig0028361:2127-2146 Msa1437730:intron 40.0%
! TCTGCTTAGTGGATAAAGCT+TGG - tig0028361:2138-2157 Msa1437730:intron 40.0%
AGCAATCTAGCAACATAGAG+GGG + tig0028361:2257-2276 None:intergenic 40.0%
TTAACTTTCAGGGATACTGC+AGG - tig0028361:2513-2532 Msa1437730:CDS 40.0%
AAACTTGCCTGTAAACCAAG+CGG + tig0028361:3222-3241 None:intergenic 40.0%
GATGCCAAGTCAAAAGAGTT+TGG - tig0028361:3256-3275 Msa1437730:intron 40.0%
GTATTGGATGAATGCACTGT+GGG + tig0028361:3680-3699 None:intergenic 40.0%
TGTATTGGATGAATGCACTG+TGG + tig0028361:3681-3700 None:intergenic 40.0%
CTTCAGTTAATTGGTGTGTG+GGG - tig0028361:3843-3862 Msa1437730:five_prime_UTR 40.0%
TTCTTAGGCGATCAATCCGT+TGG - tig0028361:1572-1591 Msa1437730:three_prime_UTR 45.0%
GAGTCGTTGAAACCCTTGAA+TGG - tig0028361:2000-2019 Msa1437730:intron 45.0%
CGAACTCTTCTTCCCATTCA+AGG + tig0028361:2016-2035 None:intergenic 45.0%
GAACTGTTCGTTTGCAGCTT+TGG - tig0028361:2353-2372 Msa1437730:intron 45.0%
!! GAACACTAACAAGTGGGTTG+AGG - tig0028361:2816-2835 Msa1437730:intron 45.0%
!! TTCACAACCGGTTTTCTGAC+TGG - tig0028361:2949-2968 Msa1437730:intron 45.0%
!! TCACAACCGGTTTTCTGACT+GGG - tig0028361:2950-2969 Msa1437730:intron 45.0%
ACCATACCCAGTCAGAAAAC+CGG + tig0028361:2959-2978 None:intergenic 45.0%
ATAGGTCAAGTGTCTTGCAG+TGG - tig0028361:3104-3123 Msa1437730:intron 45.0%
TATATGGCCGCTTGGTTTAC+AGG - tig0028361:3212-3231 Msa1437730:intron 45.0%
ATCGAAACCAGTGCAAAAGC+AGG - tig0028361:3289-3308 Msa1437730:intron 45.0%
!! ATTGAAGCCTGCTTTTGCAC+TGG + tig0028361:3299-3318 None:intergenic 45.0%
CAGGCTTCAATATCAAGGTC+AGG - tig0028361:3308-3327 Msa1437730:intron 45.0%
GAGGACACAAGAACCAACAT+GGG + tig0028361:3539-3558 None:intergenic 45.0%
TGAGGACACAAGAACCAACA+TGG + tig0028361:3540-3559 None:intergenic 45.0%
TTCATCCAATACAGAGCAGC+AGG - tig0028361:3688-3707 Msa1437730:intron 45.0%
TCATCCAATACAGAGCAGCA+GGG - tig0028361:3689-3708 Msa1437730:intron 45.0%
ACGAGCTTGTATTTGGCGAG+AGG + tig0028361:1551-1570 None:intergenic 50.0%
CATGAAGCGGGTGATGATAC+TGG + tig0028361:1603-1622 None:intergenic 50.0%
!! CACTAACAAGTGGGTTGAGG+AGG - tig0028361:2819-2838 Msa1437730:intron 50.0%
!! ACCGGTTTTCTGACTGGGTA+TGG - tig0028361:2955-2974 Msa1437730:intron 50.0%
GAAGAAAGGGTTTCCATCCC+TGG + tig0028361:3620-3639 None:intergenic 50.0%
GAGGAGGTTGCTCATGTTAG+AGG - tig0028361:3711-3730 Msa1437730:five_prime_UTR 50.0%
TGGTGTGTGGGGACAAATTG+CGG - tig0028361:3854-3873 Msa1437730:five_prime_UTR 50.0%
GTGGGGACAAATTGCGGTAA+TGG - tig0028361:3860-3879 Msa1437730:five_prime_UTR 50.0%
AAGATTGCTTCCGCCTTGCC+AGG - tig0028361:3599-3618 Msa1437730:intron 55.0%
AGATTGCTTCCGCCTTGCCA+GGG - tig0028361:3600-3619 Msa1437730:intron 55.0%
AAGGGTTTCCATCCCTGGCA+AGG + tig0028361:3615-3634 None:intergenic 55.0%
TCCAATACAGAGCAGCAGGG+AGG - tig0028361:3692-3711 Msa1437730:intron 55.0%
TCCTCCCTGCTGCTCTGTAT+TGG + tig0028361:3696-3715 None:intergenic 55.0%
AATACAGAGCAGCAGGGAGG+AGG - tig0028361:3695-3714 Msa1437730:intron 55.0%
! GAGGAGGTGCGTCAAGAACG+TGG - tig0028361:2835-2854 Msa1437730:intron 60.0%
GGTTTCCATCCCTGGCAAGG+CGG + tig0028361:3612-3631 None:intergenic 60.0%
TGCTTCCGCCTTGCCAGGGA+TGG - tig0028361:3604-3623 Msa1437730:intron 65.0%
Chromosome Type Strat End Strand Name
tig0028361 gene 1379 3973 1379 ID=Msa1437730;Name=Msa1437730
tig0028361 mRNA 1379 3973 1379 ID=Msa1437730-mRNA-1;Parent=Msa1437730;Name=Msa1437730-mRNA-1;_AED=0.07;_eAED=0.07;_QI=303|1|1|1|1|0.83|6|243|157
tig0028361 exon 1379 1765 1379 ID=Msa1437730-mRNA-1:exon:10887;Parent=Msa1437730-mRNA-1
tig0028361 exon 2028 2118 2028 ID=Msa1437730-mRNA-1:exon:10886;Parent=Msa1437730-mRNA-1
tig0028361 exon 2441 2552 2441 ID=Msa1437730-mRNA-1:exon:10885;Parent=Msa1437730-mRNA-1
tig0028361 exon 2740 2828 2740 ID=Msa1437730-mRNA-1:exon:10884;Parent=Msa1437730-mRNA-1
tig0028361 exon 2978 3047 2978 ID=Msa1437730-mRNA-1:exon:10883;Parent=Msa1437730-mRNA-1
tig0028361 exon 3703 3973 3703 ID=Msa1437730-mRNA-1:exon:10882;Parent=Msa1437730-mRNA-1
tig0028361 five_prime_UTR 3703 3973 3703 ID=Msa1437730-mRNA-1:five_prime_utr;Parent=Msa1437730-mRNA-1
tig0028361 five_prime_UTR 3016 3047 3016 ID=Msa1437730-mRNA-1:five_prime_utr;Parent=Msa1437730-mRNA-1
tig0028361 CDS 2978 3015 2978 ID=Msa1437730-mRNA-1:cds;Parent=Msa1437730-mRNA-1
tig0028361 CDS 2740 2828 2740 ID=Msa1437730-mRNA-1:cds;Parent=Msa1437730-mRNA-1
tig0028361 CDS 2441 2552 2441 ID=Msa1437730-mRNA-1:cds;Parent=Msa1437730-mRNA-1
tig0028361 CDS 2028 2118 2028 ID=Msa1437730-mRNA-1:cds;Parent=Msa1437730-mRNA-1
tig0028361 CDS 1622 1765 1622 ID=Msa1437730-mRNA-1:cds;Parent=Msa1437730-mRNA-1
tig0028361 three_prime_UTR 1379 1621 1379 ID=Msa1437730-mRNA-1:three_prime_utr;Parent=Msa1437730-mRNA-1
Gene Sequence

>Msa1437730

ATGTACCTTGAAGATAGAACTGTTCGTTTGCAGCTTTGGGATACTGCAGGCCAAGAAAGATTTAGAAGTCTCATTCCAAGCTACATAAGAGATTCTTCTGTTGCAGTTATTGTATATGATGTAGCTAACAGGCAATCATTTCTGAACACTAACAAGTGGGTTGAGGAGGTGCGTCAAGAACGTGGTAGTGATGTTATAATTGTCTTGGTTGGAAACAAAACTGATCTTGTCGAGAAAAGGCAAGTTTCTATAGAAGAAGGAGATGCCAAGTCAAAAGAGTTTGGAATCATGTTTATCGAAACCAGTGCAAAAGCAGGCTTCAATATCAAGCCTTTGTTTCGTAAGATTGCTTCCGCCTTGCCAGGGATGGAAACCCTTTCTTCTACAAATAAGGAAGACATGGTTGACGTAAATTTAAAACCCACAGTGCATTCATCCAATACAGAGCAGCAGGGAGGAGGTTGCTCATGTTAG
Protein sequence

>Msa1437730

MYLEDRTVRLQLWDTAGQERFRSLIPSYIRDSSVAVIVYDVANRQSFLNTNKWVEEVRQERGSDVIIVLVGNKTDLVEKRQVSIEEGDAKSKEFGIMFIETSAKAGFNIKPLFRKIASALPGMETLSSTNKEDMVDVNLKPTVHSSNTEQQGGGCSC*