AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0117520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0117520 MtrunA17_Chr1g0174281 99.552 223 1 0 1 223 1 223 2.15e-168 462
Msa0117520 MtrunA17_Chr7g0231131 82.883 222 35 2 1 222 1 219 5.38e-134 375
Msa0117520 MtrunA17_Chr3g0123821 72.936 218 57 2 5 222 6 221 4.58e-116 330
Msa0117520 MtrunA17_Chr8g0381841 70.642 218 63 1 5 222 7 223 7.13e-115 327
Msa0117520 MtrunA17_Chr4g0046261 61.905 210 75 2 13 222 11 215 2.46e-94 275
Msa0117520 MtrunA17_Chr2g0283131 62.381 210 74 2 13 222 11 215 2.87e-94 275
Msa0117520 MtrunA17_Chr8g0346421 66.832 202 60 2 7 207 18 213 6.26e-94 274
Msa0117520 MtrunA17_Chr4g0040781 61.905 210 75 2 13 222 11 215 2.83e-93 272
Msa0117520 MtrunA17_Chr2g0315551 61.429 210 76 3 13 222 11 215 4.78e-93 271
Msa0117520 MtrunA17_Chr4g0032201 62.617 214 74 3 9 222 6 213 1.52e-92 270
Msa0117520 MtrunA17_Chr2g0276941 61.033 213 79 2 10 222 10 218 4.32e-92 269
Msa0117520 MtrunA17_Chr2g0319951 59.070 215 84 2 9 223 7 217 5.19e-92 269
Msa0117520 MtrunA17_Chr3g0112681 60.664 211 78 2 13 223 10 215 7.07e-91 266
Msa0117520 MtrunA17_Chr7g0275991 60.465 215 79 2 10 221 15 226 2.57e-90 265
Msa0117520 MtrunA17_Chr2g0325091 58.065 217 85 2 6 222 2 212 1.28e-89 263
Msa0117520 MtrunA17_Chr8g0351861 54.867 226 98 3 1 222 1 226 5.13e-89 261
Msa0117520 MtrunA17_Chr7g0214241 55.399 213 86 3 14 223 14 220 1.77e-83 247
Msa0117520 MtrunA17_Chr1g0189361 55.399 213 90 3 13 222 12 222 4.07e-82 244
Msa0117520 MtrunA17_Chr7g0234201 53.589 209 91 1 14 222 12 214 1.09e-77 232
Msa0117520 MtrunA17_Chr8g0392931 47.907 215 108 2 8 222 7 217 7.62e-70 213
Msa0117520 MtrunA17_Chr6g0482721 47.907 215 107 2 8 222 5 214 1.22e-69 212
Msa0117520 MtrunA17_Chr2g0290891 50.000 178 89 0 14 191 5 182 8.58e-64 197
Msa0117520 MtrunA17_Chr6g0458081 48.315 178 92 0 13 190 4 181 1.01e-60 189
Msa0117520 MtrunA17_Chr8g0349711 49.718 177 89 0 14 190 5 181 2.06e-60 188
Msa0117520 MtrunA17_Chr7g0250531 48.333 180 92 1 9 187 2 181 1.06e-55 176
Msa0117520 MtrunA17_Chr8g0349681 51.497 167 81 0 14 180 383 549 1.65e-55 186
Msa0117520 MtrunA17_Chr8g0349681 47.802 182 95 0 9 190 169 350 1.69e-53 180
Msa0117520 MtrunA17_Chr8g0349681 51.923 156 75 0 14 169 12 167 1.05e-51 176
Msa0117520 MtrunA17_Chr8g0335471 42.791 215 107 4 9 222 2 201 6.96e-54 171
Msa0117520 MtrunA17_Chr2g0276931 42.180 211 108 2 13 223 7 203 8.11e-54 171
Msa0117520 MtrunA17_Chr4g0055651 48.810 168 86 0 9 176 2 169 1.13e-53 171
Msa0117520 MtrunA17_Chr1g0182321 40.654 214 113 4 13 223 13 215 3.21e-51 165
Msa0117520 MtrunA17_Chr4g0030911 41.204 216 108 4 13 223 13 214 3.76e-51 165
Msa0117520 MtrunA17_Chr1g0182301 40.187 214 114 4 13 223 13 215 1.09e-50 164
Msa0117520 MtrunA17_Chr1g0212521 39.815 216 112 3 13 223 13 215 4.25e-50 162
Msa0117520 MtrunA17_Chr7g0263071 47.273 165 86 1 13 176 13 177 1.41e-49 161
Msa0117520 MtrunA17_Chr2g0327911 42.614 176 97 3 13 184 13 188 4.85e-46 152
Msa0117520 MtrunA17_Chr1g0199331 44.172 163 90 1 17 178 33 195 6.96e-46 151
Msa0117520 MtrunA17_Chr3g0109721 43.827 162 91 0 17 178 12 173 4.52e-45 149
Msa0117520 MtrunA17_Chr5g0433561 40.782 179 98 1 17 195 12 182 3.07e-44 147
Msa0117520 MtrunA17_Chr8g0384311 37.963 216 118 6 9 222 7 208 4.26e-44 147
Msa0117520 MtrunA17_Chr5g0400131 40.127 157 94 0 16 172 10 166 2.33e-42 142
Msa0117520 MtrunA17_Chr3g0123971 33.649 211 120 2 16 222 10 204 1.18e-41 140
Msa0117520 MtrunA17_Chr8g0382221 33.333 207 127 1 16 222 10 205 1.49e-41 140
Msa0117520 MtrunA17_Chr4g0055081 38.217 157 97 0 16 172 10 166 1.58e-41 140
Msa0117520 MtrunA17_Chr1g0159481 37.209 215 118 7 11 222 13 213 9.94e-41 138
Msa0117520 MtrunA17_Chr8g0390561 38.764 178 101 1 17 194 11 180 1.78e-40 137
Msa0117520 MtrunA17_Chr3g0130271 37.037 216 119 8 10 222 7 208 3.78e-40 136
Msa0117520 MtrunA17_Chr7g0265001 37.736 212 118 3 13 222 14 213 5.14e-40 136
Msa0117520 MtrunA17_Chr1g0197331 37.557 221 113 7 9 222 9 211 4.29e-38 131
Msa0117520 MtrunA17_Chr1g0180291 37.736 212 117 5 13 222 12 210 5.71e-38 131
Msa0117520 MtrunA17_Chr8g0383261 41.818 165 89 3 15 172 12 176 1.99e-37 129
Msa0117520 MtrunA17_Chr6g0466301 42.424 165 88 3 15 172 8 172 3.07e-37 129
Msa0117520 MtrunA17_Chr6g0465491 42.424 165 88 3 15 172 8 172 3.07e-37 129
Msa0117520 MtrunA17_Chr1g0201121 42.424 165 88 3 15 172 8 172 3.19e-37 129
Msa0117520 MtrunA17_Chr4g0034871 38.503 187 108 3 15 194 8 194 2.42e-36 127
Msa0117520 MtrunA17_Chr2g0331691 37.037 189 112 3 15 196 10 198 3.07e-35 124
Msa0117520 MtrunA17_Chr4g0004661 36.508 189 113 3 15 196 64 252 1.96e-33 120
Msa0117520 MtrunA17_Chr6g0466161 39.865 148 82 3 32 172 7 154 4.28e-28 105
Msa0117520 MtrunA17_Chr6g0465471 39.865 148 82 3 32 172 7 154 4.28e-28 105
Msa0117520 MtrunA17_Chr3g0138101 27.523 218 144 5 10 216 7 221 1.40e-21 89.0
Msa0117520 MtrunA17_Chr3g0138121 31.098 164 109 3 10 172 7 167 2.11e-21 88.6
Msa0117520 MtrunA17_Chr3g0138111 31.098 164 109 3 10 172 7 167 2.27e-21 88.2
Msa0117520 MtrunA17_Chr2g0325101 35.152 165 93 5 17 169 8 170 3.71e-21 87.0
Msa0117520 MtrunA17_Chr4g0046211 38.462 117 69 3 17 132 8 122 7.08e-21 85.1
Msa0117520 MtrunA17_Chr4g0037021 33.140 172 87 6 17 169 8 170 1.83e-20 85.5
Msa0117520 MtrunA17_Chr8g0371501 33.333 165 96 5 17 169 8 170 1.31e-19 83.2
Msa0117520 MtrunA17_Chr5g0405791 32.530 166 98 5 16 169 7 170 6.87e-19 81.3
Msa0117520 MtrunA17_Chr6g0486041 37.607 117 70 3 17 132 8 122 2.65e-18 80.1
Msa0117520 MtrunA17_Chr3g0117931 31.515 165 99 5 17 169 10 172 3.91e-18 79.3
Msa0117520 MtrunA17_Chr6g0466431 37.374 99 53 1 15 113 8 97 1.34e-17 75.1
Msa0117520 MtrunA17_Chr6g0465611 37.374 99 53 1 15 113 8 97 1.34e-17 75.1
Msa0117520 MtrunA17_Chr1g0178291 33.121 157 98 3 20 176 433 582 7.05e-15 73.2
Msa0117520 MtrunA17_Chr5g0443631 28.070 171 113 4 16 179 105 272 4.49e-13 67.0
Msa0117520 MtrunA17_Chr2g0331841 41.379 58 34 0 84 141 2 59 1.42e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0117520 AT3G12160.1 88.341 223 23 2 1 223 1 220 2.05e-147 409
Msa0117520 AT5G47960.1 78.125 224 45 2 1 223 1 221 3.73e-131 369
Msa0117520 AT5G65270.1 68.037 219 68 2 5 222 7 224 1.70e-109 314
Msa0117520 AT4G39990.1 66.197 213 67 2 11 222 13 221 1.20e-103 299
Msa0117520 AT5G60860.1 60.930 215 79 2 9 223 7 216 9.51e-94 273
Msa0117520 AT1G16920.1 63.333 210 71 3 13 222 11 214 2.84e-93 272
Msa0117520 AT2G33870.1 61.137 211 78 2 13 223 11 217 9.89e-93 271
Msa0117520 AT4G18430.1 59.524 210 80 2 13 222 11 215 2.98e-92 270
Msa0117520 AT1G28550.1 62.381 210 75 2 13 222 11 216 5.27e-92 269
Msa0117520 AT1G06400.1 58.411 214 83 1 9 222 7 214 1.28e-91 268
Msa0117520 AT1G07410.1 60.280 214 78 2 9 222 6 212 2.20e-91 267
Msa0117520 AT3G15060.1 60.190 211 79 1 13 223 11 216 1.63e-90 265
Msa0117520 AT4G18800.1 61.905 210 72 3 13 222 11 212 2.61e-90 265
Msa0117520 AT5G45750.1 60.000 210 78 3 13 222 11 214 3.55e-90 265
Msa0117520 AT1G09630.1 59.070 215 83 2 9 223 6 215 9.40e-90 263
Msa0117520 AT5G59150.1 61.009 218 71 3 10 222 7 215 1.06e-87 258
Msa0117520 AT3G46830.1 58.904 219 76 2 9 222 6 215 6.02e-87 256
Msa0117520 AT3G46830.2 58.904 219 76 2 9 222 6 215 6.02e-87 256
Msa0117520 AT1G01200.1 60.092 218 76 4 10 221 23 235 7.24e-87 257
Msa0117520 AT5G47520.1 53.394 221 97 3 4 222 3 219 7.13e-86 254
Msa0117520 AT2G43130.1 62.500 176 66 0 13 188 10 185 2.47e-82 244
Msa0117520 AT1G05810.1 53.774 212 90 2 13 222 10 215 1.89e-81 242
Msa0117520 AT1G05810.2 53.521 213 91 2 13 223 56 262 4.11e-81 243
Msa0117520 AT2G31680.1 60.795 176 69 0 13 188 10 185 1.54e-80 240
Msa0117520 AT3G07410.1 53.302 212 91 3 13 222 10 215 9.26e-77 230
Msa0117520 AT1G18200.1 49.261 203 99 1 8 206 6 208 9.19e-72 218
Msa0117520 AT1G73640.1 46.018 226 111 4 8 222 6 231 5.56e-69 211
Msa0117520 AT4G17160.1 48.341 211 98 3 14 223 5 205 1.42e-66 204
Msa0117520 AT4G17170.1 46.919 211 107 3 14 223 5 211 2.64e-65 201
Msa0117520 AT4G35860.1 44.393 214 110 3 13 223 4 211 8.69e-63 195
Msa0117520 AT5G59150.2 60.947 169 52 4 59 222 5 164 6.64e-62 191
Msa0117520 AT3G11730.1 44.843 223 104 4 1 223 1 204 2.11e-56 178
Msa0117520 AT1G02130.1 42.056 214 110 2 9 222 2 201 2.01e-55 176
Msa0117520 AT4G17530.1 43.925 214 105 4 9 222 2 200 3.24e-55 175
Msa0117520 AT5G47200.1 42.991 214 107 4 9 222 2 200 7.26e-53 169
Msa0117520 AT3G09900.1 42.925 212 110 4 13 222 13 215 8.63e-51 164
Msa0117520 AT3G46060.2 41.589 214 111 4 13 223 13 215 1.40e-50 164
Msa0117520 AT3G46060.3 41.589 214 111 4 13 223 13 215 1.40e-50 164
Msa0117520 AT3G46060.1 41.589 214 111 4 13 223 13 215 1.40e-50 164
Msa0117520 AT5G03520.1 42.791 215 104 5 13 222 13 213 2.14e-50 163
Msa0117520 AT5G59840.1 41.860 215 109 5 13 223 13 215 6.11e-50 162
Msa0117520 AT3G53610.2 38.991 218 111 3 13 223 13 215 6.49e-49 159
Msa0117520 AT3G53610.3 38.991 218 111 3 13 223 13 215 6.49e-49 159
Msa0117520 AT3G53610.1 38.991 218 111 3 13 223 13 215 6.49e-49 159
Msa0117520 AT3G54840.1 46.626 163 86 1 17 178 35 197 2.13e-48 158
Msa0117520 AT4G35860.2 44.118 170 86 3 57 223 2 165 4.22e-48 155
Msa0117520 AT3G54840.2 48.052 154 79 1 17 169 35 188 5.04e-48 156
Msa0117520 AT4G19640.1 45.399 163 89 0 17 179 12 174 5.76e-48 157
Msa0117520 AT3G54840.3 46.296 162 86 1 18 178 2 163 8.33e-48 155
Msa0117520 AT5G45130.1 44.172 163 91 0 17 179 12 174 4.05e-47 154
Msa0117520 AT5G45130.2 44.099 161 90 0 19 179 22 182 6.89e-46 151
Msa0117520 AT1G43890.3 41.451 193 105 5 11 201 9 195 5.25e-44 147
Msa0117520 AT1G43890.1 41.451 193 105 5 11 201 9 195 5.25e-44 147
Msa0117520 AT1G43890.2 41.451 193 105 5 11 201 9 195 5.25e-44 147
Msa0117520 AT2G44610.1 34.135 208 127 2 16 223 10 207 2.94e-42 142
Msa0117520 AT5G10260.1 33.333 207 128 2 16 222 10 206 2.91e-41 140
Msa0117520 AT2G22290.1 32.850 207 128 1 16 222 10 205 1.26e-40 138
Msa0117520 AT4G39890.1 37.975 158 97 1 16 172 10 167 4.14e-40 137
Msa0117520 AT5G03520.2 40.223 179 88 5 50 223 40 204 1.70e-38 132
Msa0117520 AT5G03530.2 42.105 171 96 3 13 181 11 180 4.48e-38 132
Msa0117520 AT5G03530.1 42.105 171 96 3 13 181 11 180 4.48e-38 132
Msa0117520 AT5G64990.1 36.646 161 102 0 16 176 8 168 1.81e-37 130
Msa0117520 AT2G21880.1 42.424 165 88 3 15 172 9 173 3.31e-37 129
Msa0117520 AT1G22740.1 41.818 165 89 3 15 172 8 172 4.17e-37 129
Msa0117520 AT4G19640.3 37.736 159 98 1 17 175 12 169 4.52e-36 126
Msa0117520 AT1G52280.1 38.095 189 110 3 15 196 8 196 5.48e-36 126
Msa0117520 AT4G09720.1 41.818 165 89 3 15 172 8 172 1.20e-35 125
Msa0117520 AT1G49300.2 37.968 187 109 3 15 194 8 194 4.60e-35 124
Msa0117520 AT1G49300.1 37.968 187 109 3 15 194 8 194 4.60e-35 124
Msa0117520 AT4G19640.2 44.000 125 70 0 17 141 12 136 5.19e-35 122
Msa0117520 AT5G64990.2 35.119 168 102 1 16 176 8 175 8.98e-35 123
Msa0117520 AT3G18820.1 37.968 187 109 3 15 194 8 194 9.97e-35 123
Msa0117520 AT4G09720.4 40.588 170 89 3 15 172 8 177 1.57e-34 122
Msa0117520 AT4G09720.3 39.205 176 89 3 15 172 8 183 1.63e-33 120
Msa0117520 AT3G16100.1 39.394 165 93 3 15 172 8 172 1.71e-32 117
Msa0117520 AT3G09910.3 39.766 171 100 3 13 181 11 180 4.98e-32 116
Msa0117520 AT3G09910.1 39.766 171 100 3 13 181 11 180 4.98e-32 116
Msa0117520 AT3G09910.2 39.766 171 100 3 13 181 11 180 4.98e-32 116
Msa0117520 AT3G09910.5 41.509 159 90 3 13 169 11 168 1.11e-31 115
Msa0117520 AT3G09910.4 41.509 159 90 3 13 169 11 168 1.11e-31 115
Msa0117520 AT5G39620.1 33.803 213 120 5 16 221 6 204 7.66e-31 113
Msa0117520 AT5G46025.1 50.000 102 50 1 77 177 6 107 8.50e-30 107
Msa0117520 AT2G21880.4 40.541 148 81 3 32 172 1 148 4.32e-29 108
Msa0117520 AT2G21880.3 40.541 148 81 3 32 172 1 148 4.32e-29 108
Msa0117520 AT2G21880.2 37.576 165 88 4 15 172 9 165 1.92e-27 104
Msa0117520 AT4G09720.6 42.963 135 70 3 45 172 4 138 1.67e-26 100
Msa0117520 AT4G09720.5 42.963 135 70 3 45 172 4 138 1.67e-26 100
Msa0117520 AT4G09720.2 42.963 135 70 3 45 172 4 138 1.67e-26 100
Msa0117520 AT5G55080.1 30.952 168 112 3 10 176 7 171 1.50e-24 97.1
Msa0117520 AT2G44690.1 37.952 166 87 6 17 169 20 182 1.24e-23 94.4
Msa0117520 AT5G20020.1 31.098 164 109 3 10 172 7 167 2.91e-21 88.6
Msa0117520 AT5G20010.1 31.098 164 109 3 10 172 7 167 3.13e-21 88.2
Msa0117520 AT5G55190.1 31.098 164 109 3 10 172 7 167 3.70e-21 88.2
Msa0117520 AT4G35020.1 33.333 165 96 5 17 169 8 170 1.54e-20 85.9
Msa0117520 AT4G35020.2 33.333 165 96 5 17 169 8 170 1.54e-20 85.9
Msa0117520 AT4G35020.3 33.333 165 96 5 17 169 8 170 1.54e-20 85.9
Msa0117520 AT1G75840.1 33.939 165 95 6 17 169 8 170 6.21e-20 84.3
Msa0117520 AT1G20090.1 33.939 165 95 6 17 169 7 169 7.03e-20 84.3
Msa0117520 AT5G45970.1 34.320 169 97 5 17 173 8 174 1.19e-19 83.6
Msa0117520 AT3G51300.1 33.333 165 96 5 17 169 8 170 1.20e-19 83.6
Msa0117520 AT5G62880.1 31.034 174 106 5 17 178 10 181 9.99e-19 81.6
Msa0117520 AT4G08190.1 59.375 64 23 1 106 169 67 127 1.57e-18 79.0
Msa0117520 AT3G48040.1 31.737 167 96 6 17 169 10 172 1.81e-18 80.9
Msa0117520 AT2G17800.2 32.727 165 97 5 17 169 8 170 1.94e-18 80.5
Msa0117520 AT2G17800.1 32.727 165 97 5 17 169 8 170 1.94e-18 80.5
Msa0117520 AT4G35950.1 36.752 117 71 3 17 132 8 122 2.27e-18 80.1
Msa0117520 AT4G35020.4 35.965 114 70 3 17 129 8 119 2.41e-18 79.0
Msa0117520 AT4G35020.5 35.965 114 70 3 17 129 8 119 2.41e-18 79.0
Msa0117520 AT4G28950.1 36.441 118 72 3 16 132 7 122 9.88e-18 79.0
Msa0117520 AT3G48040.2 36.752 117 71 3 17 132 10 124 1.97e-17 77.4
Msa0117520 AT5G62880.2 35.897 117 72 3 17 132 10 124 2.05e-17 77.4
Msa0117520 AT3G22950.1 33.898 118 72 4 16 132 18 130 2.28e-11 60.8
Msa0117520 AT3G22950.2 33.898 118 72 4 16 132 18 130 2.28e-11 60.8

Find 76 sgRNAs with CRISPR-Local

Find 248 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAGATCCTGGTGGTGGTTC+AGG 0.187374 1_3:+38513434 Msa0117520:CDS
AAACTTGTTTATACAAATTC+TGG 0.216872 1_3:+38513733 Msa0117520:three_prime_UTR
TGAGGTTGAGGTTTGGGTTT+TGG 0.243210 1_3:-38510535 None:intergenic
AAACTGTTAAGGCTCAAATA+TGG 0.249016 1_3:+38510854 Msa0117520:CDS
TTCTGGCCCTTTCTGGTTAT+AGG 0.255671 1_3:+38513706 Msa0117520:three_prime_UTR
CTTGCTTGTTGTGATCTTCT+TGG 0.260302 1_3:+38513543 Msa0117520:three_prime_UTR
CCAGAAAGGGCCAGAAAATA+AGG 0.273026 1_3:-38513699 None:intergenic
TTGTGTTGAGGTTGAGGTTT+GGG 0.282724 1_3:-38510541 None:intergenic
AATATTGTCATAATGCTAAT+TGG 0.297275 1_3:+38513221 Msa0117520:CDS
ATTATCAATAACAAGAGTTT+TGG 0.313881 1_3:-38510830 None:intergenic
TAATTTGTAGCAACTAGTTT+AGG 0.321101 1_3:+38513804 Msa0117520:three_prime_UTR
AAAGGTGCAGTTTCGTTGTT+GGG 0.324797 1_3:-38510473 None:intergenic
CATAGGATGTCGAATTTATA+CGG 0.347484 1_3:+38510664 Msa0117520:exon
ATTGGCAACAAGTGTGATTT+GGG 0.348821 1_3:+38513239 Msa0117520:CDS
ATGGAAACAGTGTAACTTAC+TGG 0.358344 1_3:+38513774 Msa0117520:three_prime_UTR
CGACGACGTTTCTTTCTGTA+TGG 0.361778 1_3:-38510499 None:intergenic
TTGCTTGTTGTGATCTTCTT+GGG 0.366484 1_3:+38513544 Msa0117520:three_prime_UTR
CAAAGGTGCAGTTTCGTTGT+TGG 0.371395 1_3:-38510474 None:intergenic
AATTGGCAACAAGTGTGATT+TGG 0.372094 1_3:+38513238 Msa0117520:CDS
CAGATCCTGGTGGTGGTTCA+GGG 0.385083 1_3:+38513435 Msa0117520:CDS
TATTGATAATAAAACTGTTA+AGG 0.398879 1_3:+38510843 Msa0117520:CDS
AACTGTTAAGGCTCAAATAT+GGG 0.437288 1_3:+38510855 Msa0117520:CDS
TTCTGGTTCAGTTCAGATAG+AGG 0.438214 1_3:+38513750 Msa0117520:three_prime_UTR
AAAGCACTCGCATCTTGGTT+AGG 0.445772 1_3:-38513485 None:intergenic
GTTGATTCTAAAGCTACAAT+TGG 0.446862 1_3:+38510793 Msa0117520:CDS
TTTGTGTTGAGGTTGAGGTT+TGG 0.448590 1_3:-38510542 None:intergenic
TCACATGGCAAGATGGTTAG+AGG 0.456753 1_3:+38513175 Msa0117520:CDS
TTTCAAAAGCACTCGCATCT+TGG 0.457557 1_3:-38513490 None:intergenic
ACCAGGATCTGCCTCATCAT+TGG 0.458276 1_3:-38513423 None:intergenic
AGGGCCAGAAAATAAGGAAA+TGG 0.475848 1_3:-38513693 None:intergenic
TGGTGGTTCAGGGCTTCTGA+AGG 0.476345 1_3:+38513445 Msa0117520:CDS
TTGATTGGTGATTCTGCCGT+TGG 0.485705 1_3:+38510727 Msa0117520:CDS
TTTAAATCCTATAACCAGAA+AGG 0.486552 1_3:-38513713 None:intergenic
TTTCTTTCTGTATGGGTCAA+AGG 0.487672 1_3:-38510491 None:intergenic
TGCAGTTTCGTTGTTGGGGA+TGG 0.491981 1_3:-38510468 None:intergenic
CTGATGTCTCCATAAAGAAA+AGG 0.498679 1_3:-38513316 None:intergenic
AAAGGCGGTTTCGACATTAG+TGG 0.498739 1_3:-38513348 None:intergenic
TTAAATCCTATAACCAGAAA+GGG 0.499141 1_3:-38513712 None:intergenic
GGCTCTGCCTACATGTGGGT+TGG 0.499271 1_3:+38513585 Msa0117520:three_prime_UTR
GTGTGATTTGGGAACTCTAA+AGG 0.501914 1_3:+38513250 Msa0117520:CDS
TAATCATGAAGAGAATGAAA+AGG 0.502459 1_3:-38510572 None:intergenic
CAGGGCTTCTGAAGGGAACC+AGG 0.508518 1_3:+38513453 Msa0117520:CDS
GGGAAGCTTATTGCTCACTC+TGG 0.508994 1_3:+38513564 Msa0117520:three_prime_UTR
AGTACCTACTGAAGATGCTC+AGG 0.512755 1_3:+38513274 Msa0117520:CDS
CATTTGATCACATGGCAAGA+TGG 0.515877 1_3:+38513168 Msa0117520:CDS
TTGGTTAGGAACAATTATCC+TGG 0.519414 1_3:-38513471 None:intergenic
CAATCTCATCAGCAAAATGT+AGG 0.527732 1_3:+38513628 Msa0117520:three_prime_UTR
TTCTGTTAGAATAGTCAGAA+AGG 0.528911 1_3:-38513366 None:intergenic
ACTCTGGCTCTGCCTACATG+TGG 0.536353 1_3:+38513580 Msa0117520:three_prime_UTR
CTCTGGCTCTGCCTACATGT+GGG 0.541629 1_3:+38513581 Msa0117520:three_prime_UTR
GGTGGTTCAGGGCTTCTGAA+GGG 0.541689 1_3:+38513446 Msa0117520:CDS
GCGTATTACCGTGGAGCTGT+TGG 0.546431 1_3:+38513110 Msa0117520:CDS
GCCAATGATGAGGCAGATCC+TGG 0.550258 1_3:+38513422 Msa0117520:CDS
GGGACACTGCTGGTCAAGAA+AGG 0.550300 1_3:+38510875 Msa0117520:CDS
GACGACGTTTCTTTCTGTAT+GGG 0.552948 1_3:-38510498 None:intergenic
AAGAGAAGCAAATTTACCAT+AGG 0.553711 1_3:+38510365 Msa0117520:five_prime_UTR
AGAAGCCCTGAACCACCACC+AGG 0.558428 1_3:-38513440 None:intergenic
TGAGAAAGAATCTAAAGTGC+AGG 0.559163 1_3:+38513847 Msa0117520:three_prime_UTR
GCTCAAATATGGGACACTGC+TGG 0.573380 1_3:+38510865 Msa0117520:CDS
TAATTGCTATGATAATGATA+AGG 0.573458 1_3:-38510604 None:intergenic
AGATCACAACAAGCAAGAGT+AGG 0.583275 1_3:-38513537 None:intergenic
GAAAAGAAGGGTGGTTGCTG+CGG 0.586510 1_3:+38513509 Msa0117520:CDS
GCGTCAATCATTTGATCACA+TGG 0.593625 1_3:+38513160 Msa0117520:CDS
AAGGTGCAGTTTCGTTGTTG+GGG 0.600330 1_3:-38510472 None:intergenic
AAGATGGTTAGAGGAACTGA+GGG 0.608152 1_3:+38513184 Msa0117520:CDS
ATGTTATGTTATGATGATGA+CGG 0.609812 1_3:-38510342 None:intergenic
AACACTTACTGCCAATGATG+AGG 0.617734 1_3:+38513412 Msa0117520:CDS
GTTCAGTTCAGATAGAGGAA+TGG 0.624925 1_3:+38513755 Msa0117520:three_prime_UTR
CAAGATGGTTAGAGGAACTG+AGG 0.635134 1_3:+38513183 Msa0117520:CDS
GATGAGGCAGATCCTGGTGG+TGG 0.641867 1_3:+38513428 Msa0117520:CDS
ACATCGCTCCAACAGCTCCA+CGG 0.650042 1_3:-38513118 None:intergenic
AACTCCTGAGCATCTTCAGT+AGG 0.655334 1_3:-38513278 None:intergenic
GTAACTAGTGCGTATTACCG+TGG 0.661026 1_3:+38513101 Msa0117520:CDS
TGTTAGAATAGTCAGAAAGG+CGG 0.662740 1_3:-38513363 None:intergenic
AATGATGAGGCAGATCCTGG+TGG 0.756314 1_3:+38513425 Msa0117520:CDS
AGATGGTTAGAGGAACTGAG+GGG 0.801297 1_3:+38513185 Msa0117520:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTCTTTTTGTATTTTTTTT+TGG - chr1_3:38510427-38510446 None:intergenic 10.0%
!!! TTTGTTCAATTTTTAATCAT+AGG + chr1_3:38510647-38510666 Msa0117520:five_prime_UTR 15.0%
!!! TATTGATAATAAAACTGTTA+AGG + chr1_3:38510843-38510862 Msa0117520:CDS 15.0%
!!! TTAAATGATAATGATAATGA+TGG + chr1_3:38510988-38511007 Msa0117520:intron 15.0%
!! TGAAATGTAATTTACAAATT+GGG + chr1_3:38511733-38511752 Msa0117520:intron 15.0%
!!! TTCAGTGATATTATTAATTT+TGG - chr1_3:38512039-38512058 None:intergenic 15.0%
!!! TATTTAGAATACAACATATT+AGG + chr1_3:38512177-38512196 Msa0117520:intron 15.0%
!! TTATTAGAAAACAATATAAG+CGG - chr1_3:38512456-38512475 None:intergenic 15.0%
!!! TTATATTGTTTTCTAATAAG+AGG + chr1_3:38512457-38512476 Msa0117520:intron 15.0%
!! TAAATGAAAGAAATATTGTT+TGG + chr1_3:38512594-38512613 Msa0117520:intron 15.0%
!!! GAAAAAATCATTTTTAGATA+TGG + chr1_3:38512630-38512649 Msa0117520:intron 15.0%
!!! TAATTGCTATGATAATGATA+AGG - chr1_3:38510607-38510626 None:intergenic 20.0%
!!! ATTATCAATAACAAGAGTTT+TGG - chr1_3:38510833-38510852 None:intergenic 20.0%
!! TGAATAATTTATGATGTGTT+CGG - chr1_3:38511023-38511042 None:intergenic 20.0%
!! GGTAATAAAAAATGTATTAC+TGG + chr1_3:38511570-38511589 Msa0117520:intron 20.0%
!! CTGAAATGTAATTTACAAAT+TGG + chr1_3:38511732-38511751 Msa0117520:intron 20.0%
!!! TAATGTATCATATGTTTTGA+GGG + chr1_3:38511762-38511781 Msa0117520:intron 20.0%
!! TCAAATTTGAATACAATTTG+TGG + chr1_3:38511785-38511804 Msa0117520:intron 20.0%
!! TTAGGAAATAAAACTAACTA+AGG + chr1_3:38512195-38512214 Msa0117520:intron 20.0%
!! ATTCTTGAATTTCTTGAAAA+AGG - chr1_3:38512259-38512278 None:intergenic 20.0%
!! GATAGTTATTGAATGATTAT+GGG - chr1_3:38512326-38512345 None:intergenic 20.0%
!! TTTGTTAATAAATATGCAAC+TGG + chr1_3:38512700-38512719 Msa0117520:intron 20.0%
!! AATATTGTCATAATGCTAAT+TGG + chr1_3:38513221-38513240 Msa0117520:CDS 20.0%
!!! AAACTTGTTTATACAAATTC+TGG + chr1_3:38513733-38513752 Msa0117520:three_prime_UTR 20.0%
!! ATGTTATGTTATGATGATGA+CGG - chr1_3:38510345-38510364 None:intergenic 25.0%
! TAATCATGAAGAGAATGAAA+AGG - chr1_3:38510575-38510594 None:intergenic 25.0%
!!! TATTTGATTTCATGTCGTTT+TGG + chr1_3:38510947-38510966 Msa0117520:intron 25.0%
!! AATGATAATGATAATGATGG+TGG + chr1_3:38510991-38511010 Msa0117520:intron 25.0%
!! ATGATAATGATAATGATGGT+GGG + chr1_3:38510992-38511011 Msa0117520:intron 25.0%
!!! TGAGAAATTTTGGTTGTAAA+GGG + chr1_3:38511384-38511403 Msa0117520:intron 25.0%
! GAAACTTCATACTTATAGTA+TGG + chr1_3:38511549-38511568 Msa0117520:intron 25.0%
! AGTAATAAATTACACAGACA+CGG - chr1_3:38511624-38511643 None:intergenic 25.0%
!! GTAATGTATCATATGTTTTG+AGG + chr1_3:38511761-38511780 Msa0117520:intron 25.0%
!! GACACTCTGTTTTATATTTA+CGG - chr1_3:38512104-38512123 None:intergenic 25.0%
! TTCATTTCTTTCCTAAAAAG+AGG - chr1_3:38512126-38512145 None:intergenic 25.0%
! AGGAAAGAAATGAAAATGAA+TGG + chr1_3:38512132-38512151 Msa0117520:intron 25.0%
! ATGAAAATGAATGGAATCTA+CGG + chr1_3:38512141-38512160 Msa0117520:intron 25.0%
! TCTACTTGTTTGAACTTTAT+AGG + chr1_3:38512228-38512247 Msa0117520:intron 25.0%
! AAACCAACTAATTCTTAGAA+GGG - chr1_3:38512292-38512311 None:intergenic 25.0%
! GGATAGTTATTGAATGATTA+TGG - chr1_3:38512327-38512346 None:intergenic 25.0%
!!! TTTTAGGAATAAACAGAGAA+AGG - chr1_3:38512368-38512387 None:intergenic 25.0%
!! TGTCAGTAATGCTTAATTTT+AGG - chr1_3:38512384-38512403 None:intergenic 25.0%
! TATTTGGAGGAATGATATTT+AGG + chr1_3:38512512-38512531 Msa0117520:intron 25.0%
!! GATATGGAAATACTTTTAGA+AGG + chr1_3:38512646-38512665 Msa0117520:intron 25.0%
! GAAAATGTGAAGAATTGATT+TGG - chr1_3:38512755-38512774 None:intergenic 25.0%
!! ATGTTCTAAACAATTTCTAG+TGG + chr1_3:38512794-38512813 Msa0117520:intron 25.0%
!!! GAATGTTTGAGACTTTTATT+TGG + chr1_3:38513027-38513046 Msa0117520:intron 25.0%
! TTAAATCCTATAACCAGAAA+GGG - chr1_3:38513715-38513734 None:intergenic 25.0%
! TTTAAATCCTATAACCAGAA+AGG - chr1_3:38513716-38513735 None:intergenic 25.0%
!! TAATTTGTAGCAACTAGTTT+AGG + chr1_3:38513804-38513823 Msa0117520:three_prime_UTR 25.0%
!!! GATGATGACGGTTTTTTTTA+GGG - chr1_3:38510333-38510352 None:intergenic 30.0%
!!! TGATGATGACGGTTTTTTTT+AGG - chr1_3:38510334-38510353 None:intergenic 30.0%
AAGAGAAGCAAATTTACCAT+AGG + chr1_3:38510365-38510384 Msa0117520:five_prime_UTR 30.0%
! TCGATTTTCGATATAACCTA+TGG - chr1_3:38510384-38510403 None:intergenic 30.0%
CATAGGATGTCGAATTTATA+CGG + chr1_3:38510664-38510683 Msa0117520:exon 30.0%
! AAGAAGTTGTGTTTTTCCAA+CGG - chr1_3:38510746-38510765 None:intergenic 30.0%
!! GTTGATTCTAAAGCTACAAT+TGG + chr1_3:38510793-38510812 Msa0117520:CDS 30.0%
AAACTGTTAAGGCTCAAATA+TGG + chr1_3:38510854-38510873 Msa0117520:CDS 30.0%
AACTGTTAAGGCTCAAATAT+GGG + chr1_3:38510855-38510874 Msa0117520:CDS 30.0%
TAATTTATGATGTGTTCGGA+GGG - chr1_3:38511019-38511038 None:intergenic 30.0%
ATAATTTATGATGTGTTCGG+AGG - chr1_3:38511020-38511039 None:intergenic 30.0%
! AATTTCTATACGCTCTCTTT+TGG - chr1_3:38511117-38511136 None:intergenic 30.0%
! GAAGTATTACTTGTGTCAAA+TGG + chr1_3:38511214-38511233 Msa0117520:intron 30.0%
!! GGATTTGGGTCTTTTTATTT+TGG + chr1_3:38511241-38511260 Msa0117520:intron 30.0%
!!! CTGAGAAATTTTGGTTGTAA+AGG + chr1_3:38511383-38511402 Msa0117520:intron 30.0%
AAGTTTCCAACAATGTTTAG+TGG - chr1_3:38511536-38511555 None:intergenic 30.0%
GTGTCTGTGTAATTTATTAC+TGG + chr1_3:38511623-38511642 Msa0117520:intron 30.0%
CCTCATTGTATTACTACTTA+TGG - chr1_3:38511693-38511712 None:intergenic 30.0%
CCATAAGTAGTAATACAATG+AGG + chr1_3:38511690-38511709 Msa0117520:intron 30.0%
TAAGTAGTAATACAATGAGG+TGG + chr1_3:38511693-38511712 Msa0117520:intron 30.0%
CAACATGCAACAATTGATAA+AGG - chr1_3:38511819-38511838 None:intergenic 30.0%
CATGTTGTTGATATAGTTGT+GGG + chr1_3:38511832-38511851 Msa0117520:intron 30.0%
! TCTTTTATCGATGAGTTACA+AGG + chr1_3:38512066-38512085 Msa0117520:intron 30.0%
CAAACCAACTAATTCTTAGA+AGG - chr1_3:38512293-38512312 None:intergenic 30.0%
! CTTCTAAGAATTAGTTGGTT+TGG + chr1_3:38512291-38512310 Msa0117520:intron 30.0%
GTTATTGAATGATTATGGGA+TGG - chr1_3:38512322-38512341 None:intergenic 30.0%
GAATAAACAGAGAAAGGAAA+GGG - chr1_3:38512362-38512381 None:intergenic 30.0%
AACATGCAAACCAATATGTA+TGG - chr1_3:38512779-38512798 None:intergenic 30.0%
! GCCTTCTTTTCATTTATCTT+TGG + chr1_3:38512925-38512944 Msa0117520:intron 30.0%
GCCAAAGATAAATGAAAAGA+AGG - chr1_3:38512929-38512948 None:intergenic 30.0%
! AAGAGAGAACCTTTTCTTTA+TGG + chr1_3:38513307-38513326 Msa0117520:CDS 30.0%
TTCTGTTAGAATAGTCAGAA+AGG - chr1_3:38513369-38513388 None:intergenic 30.0%
!!! GTGTTTTTTTGCTGATAAGT+CGG - chr1_3:38513397-38513416 None:intergenic 30.0%
!!! CATACCATTTCCTTATTTTC+TGG + chr1_3:38513689-38513708 Msa0117520:three_prime_UTR 30.0%
TTTCTTTCTGTATGGGTCAA+AGG - chr1_3:38510494-38510513 None:intergenic 35.0%
! AAGGTTTGTGTTTTGTGTTG+AGG - chr1_3:38510556-38510575 None:intergenic 35.0%
! GATCGATTACGTTTTCAAAG+TGG + chr1_3:38510702-38510721 Msa0117520:CDS 35.0%
!!! GTTTTCAAAGTGGTGTTGAT+TGG + chr1_3:38510712-38510731 Msa0117520:CDS 35.0%
! TGAATTGAAGGTGTGTTTGA+TGG + chr1_3:38511163-38511182 Msa0117520:intron 35.0%
ATTACTTGTGTCAAATGGCA+AGG + chr1_3:38511219-38511238 Msa0117520:intron 35.0%
TTACTTGTGTCAAATGGCAA+GGG + chr1_3:38511220-38511239 Msa0117520:intron 35.0%
!!! GGGTCTTTTTATTTTGGTGA+TGG + chr1_3:38511247-38511266 Msa0117520:intron 35.0%
!!! CTTTTTATTTTGGTGATGGC+CGG + chr1_3:38511251-38511270 Msa0117520:intron 35.0%
!!! TTTTTATTTTGGTGATGGCC+GGG + chr1_3:38511252-38511271 Msa0117520:intron 35.0%
TGCATAAAACATGCAATGTC+TGG - chr1_3:38511295-38511314 None:intergenic 35.0%
!!! TATGGGGTACTGAGAAATTT+TGG + chr1_3:38511374-38511393 Msa0117520:intron 35.0%
TCTCTACCGAGAAATCTATT+GGG - chr1_3:38511427-38511446 None:intergenic 35.0%
ATCTCTACCGAGAAATCTAT+TGG - chr1_3:38511428-38511447 None:intergenic 35.0%
ATAAAACTTGTGTGCCAAAG+AGG + chr1_3:38511489-38511508 Msa0117520:intron 35.0%
TCTCCTTCAAAAAACCTCTT+TGG - chr1_3:38511506-38511525 None:intergenic 35.0%
TAGTCACCACTAAACATTGT+TGG + chr1_3:38511527-38511546 Msa0117520:intron 35.0%
GCATGTTGTTGATATAGTTG+TGG + chr1_3:38511831-38511850 Msa0117520:intron 35.0%
!! AAACAGAGTGTCCTCTTTTT+AGG + chr1_3:38512112-38512131 Msa0117520:intron 35.0%
! TCTCCCTTCTAAGAATTAGT+TGG + chr1_3:38512286-38512305 Msa0117520:intron 35.0%
AGGAAAGGGAAAGTGATAAT+GGG - chr1_3:38512348-38512367 None:intergenic 35.0%
AAGGAAAGGGAAAGTGATAA+TGG - chr1_3:38512349-38512368 None:intergenic 35.0%
GGAATAAACAGAGAAAGGAA+AGG - chr1_3:38512363-38512382 None:intergenic 35.0%
GAAAACAATATAAGCGGCAA+TGG - chr1_3:38512450-38512469 None:intergenic 35.0%
!!! GTTCTTGAGGTTTTATTTGG+AGG + chr1_3:38512499-38512518 Msa0117520:intron 35.0%
! CACATTTTCGCCATACATAT+TGG + chr1_3:38512766-38512785 Msa0117520:intron 35.0%
!! ACAATTTCTAGTGGTGCAAT+TGG + chr1_3:38512803-38512822 Msa0117520:intron 35.0%
TCTAAGTAACCTACATCGTT+CGG + chr1_3:38512864-38512883 Msa0117520:intron 35.0%
!!! TTTTTTTGACCGAACGATGT+AGG - chr1_3:38512876-38512895 None:intergenic 35.0%
!! GAGACTTTTATTTGGTACTC+TGG + chr1_3:38513035-38513054 Msa0117520:intron 35.0%
AATTGGCAACAAGTGTGATT+TGG + chr1_3:38513238-38513257 Msa0117520:CDS 35.0%
ATTGGCAACAAGTGTGATTT+GGG + chr1_3:38513239-38513258 Msa0117520:CDS 35.0%
CTGATGTCTCCATAAAGAAA+AGG - chr1_3:38513319-38513338 None:intergenic 35.0%
TGTTAGAATAGTCAGAAAGG+CGG - chr1_3:38513366-38513385 None:intergenic 35.0%
TTGGTTAGGAACAATTATCC+TGG - chr1_3:38513474-38513493 None:intergenic 35.0%
!! TTGCTTGTTGTGATCTTCTT+GGG + chr1_3:38513544-38513563 Msa0117520:three_prime_UTR 35.0%
CAATCTCATCAGCAAAATGT+AGG + chr1_3:38513628-38513647 Msa0117520:three_prime_UTR 35.0%
ATGGAAACAGTGTAACTTAC+TGG + chr1_3:38513774-38513793 Msa0117520:three_prime_UTR 35.0%
! AAAGGTGCAGTTTCGTTGTT+GGG - chr1_3:38510476-38510495 None:intergenic 40.0%
GACGACGTTTCTTTCTGTAT+GGG - chr1_3:38510501-38510520 None:intergenic 40.0%
!!! TGAGGTTTGGGTTTTGGTTT+GGG - chr1_3:38510532-38510551 None:intergenic 40.0%
!!! TTGAGGTTTGGGTTTTGGTT+TGG - chr1_3:38510533-38510552 None:intergenic 40.0%
TTGTGTTGAGGTTGAGGTTT+GGG - chr1_3:38510544-38510563 None:intergenic 40.0%
TTTGTGTTGAGGTTGAGGTT+TGG - chr1_3:38510545-38510564 None:intergenic 40.0%
! TGTGTTTTGTGTTGAGGTTG+AGG - chr1_3:38510550-38510569 None:intergenic 40.0%
!! AACTTCTTGCTCGTTTTTCG+AGG + chr1_3:38510758-38510777 Msa0117520:CDS 40.0%
GCGTCGAAACTTTGAATTGA+AGG + chr1_3:38511151-38511170 Msa0117520:intron 40.0%
! GGTGTGTTTGATGGTTTATG+TGG + chr1_3:38511172-38511191 Msa0117520:intron 40.0%
TGTCAAATGGCAAGGGATTT+GGG + chr1_3:38511227-38511246 Msa0117520:intron 40.0%
!!! TTTTATTTTGGTGATGGCCG+GGG + chr1_3:38511253-38511272 Msa0117520:intron 40.0%
TGGGTCTTTAAGCAAGTGTA+TGG + chr1_3:38511356-38511375 Msa0117520:intron 40.0%
GGGTCTTTAAGCAAGTGTAT+GGG + chr1_3:38511357-38511376 Msa0117520:intron 40.0%
GGTCTTTAAGCAAGTGTATG+GGG + chr1_3:38511358-38511377 Msa0117520:intron 40.0%
ATCTATTGGGCACACAAGAT+GGG - chr1_3:38511414-38511433 None:intergenic 40.0%
AATCTATTGGGCACACAAGA+TGG - chr1_3:38511415-38511434 None:intergenic 40.0%
TGTGTGCCCAATAGATTTCT+CGG + chr1_3:38511418-38511437 Msa0117520:intron 40.0%
! TTCTCGGTAGAGATTAGTCA+CGG + chr1_3:38511434-38511453 Msa0117520:intron 40.0%
!!! GTGCCAAAGAGGTTTTTTGA+AGG + chr1_3:38511500-38511519 Msa0117520:intron 40.0%
ATTACTGGTGTCTATGCGTA+AGG + chr1_3:38511585-38511604 Msa0117520:intron 40.0%
TCTATGCGTAAGGTTGTGTT+TGG + chr1_3:38511595-38511614 Msa0117520:intron 40.0%
TATTACTGGTGTCTATGCGT+AGG + chr1_3:38511584-38511603 Msa0117520:intron 40.0%
GTAGTAATACAATGAGGTGG+TGG + chr1_3:38511696-38511715 Msa0117520:intron 40.0%
TGATATAGTTGTGGGTGCTT+TGG + chr1_3:38511840-38511859 Msa0117520:intron 40.0%
GATATAGTTGTGGGTGCTTT+GGG + chr1_3:38511841-38511860 Msa0117520:intron 40.0%
TGTTCAATGGTTTCACCTTG+TGG + chr1_3:38511879-38511898 Msa0117520:intron 40.0%
GTTCAATGGTTTCACCTTGT+GGG + chr1_3:38511880-38511899 Msa0117520:intron 40.0%
TGCATGTCCTAAATCTCACA+AGG + chr1_3:38511966-38511985 Msa0117520:intron 40.0%
GTTGCTTCCTTGTGAGATTT+AGG - chr1_3:38511976-38511995 None:intergenic 40.0%
CAATCCTAACAGATGAGTCT+TGG - chr1_3:38512011-38512030 None:intergenic 40.0%
CAAGACTCATCTGTTAGGAT+TGG + chr1_3:38512009-38512028 Msa0117520:intron 40.0%
GAGGTCTATCATCATCTTAG+CGG + chr1_3:38512476-38512495 Msa0117520:intron 40.0%
AGGTCTATCATCATCTTAGC+GGG + chr1_3:38512477-38512496 Msa0117520:intron 40.0%
!!! CGGGTTCTTGAGGTTTTATT+TGG + chr1_3:38512496-38512515 Msa0117520:intron 40.0%
AAGAAGGCAACATACGATGT+AGG - chr1_3:38512913-38512932 None:intergenic 40.0%
ACCTAAGCTAATGCAGAAGA+AGG - chr1_3:38512955-38512974 None:intergenic 40.0%
ATCTCTTCTCTCATTCTCTC+AGG + chr1_3:38513069-38513088 Msa0117520:intron 40.0%
GCGTCAATCATTTGATCACA+TGG + chr1_3:38513160-38513179 Msa0117520:CDS 40.0%
CATTTGATCACATGGCAAGA+TGG + chr1_3:38513168-38513187 Msa0117520:CDS 40.0%
AAGATGGTTAGAGGAACTGA+GGG + chr1_3:38513184-38513203 Msa0117520:CDS 40.0%
! GTGTGATTTGGGAACTCTAA+AGG + chr1_3:38513250-38513269 Msa0117520:CDS 40.0%
AACACTTACTGCCAATGATG+AGG + chr1_3:38513412-38513431 Msa0117520:CDS 40.0%
!! TTTCAAAAGCACTCGCATCT+TGG - chr1_3:38513493-38513512 None:intergenic 40.0%
!! TGCGAGTGCTTTTGAAAAGA+AGG + chr1_3:38513496-38513515 Msa0117520:CDS 40.0%
!! GCGAGTGCTTTTGAAAAGAA+GGG + chr1_3:38513497-38513516 Msa0117520:CDS 40.0%
!! AGTGCTTTTGAAAAGAAGGG+TGG + chr1_3:38513500-38513519 Msa0117520:CDS 40.0%
AGATCACAACAAGCAAGAGT+AGG - chr1_3:38513540-38513559 None:intergenic 40.0%
!! CTTGCTTGTTGTGATCTTCT+TGG + chr1_3:38513543-38513562 Msa0117520:three_prime_UTR 40.0%
AAAAACACCAACCCACATGT+AGG - chr1_3:38513595-38513614 None:intergenic 40.0%
!!! TGGGTTGGTGTTTTTAGAGA+GGG + chr1_3:38513600-38513619 Msa0117520:three_prime_UTR 40.0%
AGGGCCAGAAAATAAGGAAA+TGG - chr1_3:38513696-38513715 None:intergenic 40.0%
!! TTCTGGTTCAGTTCAGATAG+AGG + chr1_3:38513750-38513769 Msa0117520:three_prime_UTR 40.0%
GTTCAGTTCAGATAGAGGAA+TGG + chr1_3:38513755-38513774 Msa0117520:three_prime_UTR 40.0%
GGGATGGTGAAAAACTCGAT+CGG - chr1_3:38510455-38510474 None:intergenic 45.0%
AAGGTGCAGTTTCGTTGTTG+GGG - chr1_3:38510475-38510494 None:intergenic 45.0%
! CAAAGGTGCAGTTTCGTTGT+TGG - chr1_3:38510477-38510496 None:intergenic 45.0%
CGACGACGTTTCTTTCTGTA+TGG - chr1_3:38510502-38510521 None:intergenic 45.0%
!! TGAGGTTGAGGTTTGGGTTT+TGG - chr1_3:38510538-38510557 None:intergenic 45.0%
! TTGATTGGTGATTCTGCCGT+TGG + chr1_3:38510727-38510746 Msa0117520:CDS 45.0%
GTGTCAAATGGCAAGGGATT+TGG + chr1_3:38511226-38511245 Msa0117520:intron 45.0%
! ACATGCAATGTCTGGAGTTC+CGG - chr1_3:38511287-38511306 None:intergenic 45.0%
TCAACTGAGTTAAGCTCACG+AGG + chr1_3:38511324-38511343 Msa0117520:intron 45.0%
! TTGTGGGTGCTTTGGGTAAA+GGG + chr1_3:38511848-38511867 Msa0117520:intron 45.0%
AAGGGTGGGTGAATGTTCAA+TGG + chr1_3:38511866-38511885 Msa0117520:intron 45.0%
TGAGTCTTGGCTCAACGATA+AGG - chr1_3:38511998-38512017 None:intergenic 45.0%
TGAGCCAAGACTCATCTGTT+AGG + chr1_3:38512004-38512023 Msa0117520:intron 45.0%
!! ATCATCTTAGCGGGTTCTTG+AGG + chr1_3:38512486-38512505 Msa0117520:intron 45.0%
! GCCTTCTTCTGCATTAGCTT+AGG + chr1_3:38512951-38512970 Msa0117520:intron 45.0%
GTAACTAGTGCGTATTACCG+TGG + chr1_3:38513101-38513120 Msa0117520:CDS 45.0%
TCACATGGCAAGATGGTTAG+AGG + chr1_3:38513175-38513194 Msa0117520:CDS 45.0%
CAAGATGGTTAGAGGAACTG+AGG + chr1_3:38513183-38513202 Msa0117520:CDS 45.0%
AGATGGTTAGAGGAACTGAG+GGG + chr1_3:38513185-38513204 Msa0117520:CDS 45.0%
AGTACCTACTGAAGATGCTC+AGG + chr1_3:38513274-38513293 Msa0117520:CDS 45.0%
AACTCCTGAGCATCTTCAGT+AGG - chr1_3:38513281-38513300 None:intergenic 45.0%
AAAGGCGGTTTCGACATTAG+TGG - chr1_3:38513351-38513370 None:intergenic 45.0%
! AAAGCACTCGCATCTTGGTT+AGG - chr1_3:38513488-38513507 None:intergenic 45.0%
!!! GTGGGTTGGTGTTTTTAGAG+AGG + chr1_3:38513599-38513618 Msa0117520:three_prime_UTR 45.0%
CCAGAAAGGGCCAGAAAATA+AGG - chr1_3:38513702-38513721 None:intergenic 45.0%
!!! CCTTATTTTCTGGCCCTTTC+TGG + chr1_3:38513699-38513718 Msa0117520:three_prime_UTR 45.0%
!! TTCTGGCCCTTTCTGGTTAT+AGG + chr1_3:38513706-38513725 Msa0117520:three_prime_UTR 45.0%
TGCAGTTTCGTTGTTGGGGA+TGG - chr1_3:38510471-38510490 None:intergenic 50.0%
GCTCAAATATGGGACACTGC+TGG + chr1_3:38510865-38510884 Msa0117520:CDS 50.0%
! ATGCAATGTCTGGAGTTCCG+GGG - chr1_3:38511285-38511304 None:intergenic 50.0%
! CATGCAATGTCTGGAGTTCC+GGG - chr1_3:38511286-38511305 None:intergenic 50.0%
AGCTCACGAGGACAGAGATA+TGG + chr1_3:38511336-38511355 Msa0117520:intron 50.0%
GCTCACGAGGACAGAGATAT+GGG + chr1_3:38511337-38511356 Msa0117520:intron 50.0%
GTTGTGTTTGATGCCCGTGT+CGG + chr1_3:38511659-38511678 Msa0117520:intron 50.0%
! GTTGTGGGTGCTTTGGGTAA+AGG + chr1_3:38511847-38511866 Msa0117520:intron 50.0%
TGGTTTCACCTTGTGGGTTC+TGG + chr1_3:38511886-38511905 Msa0117520:intron 50.0%
GGTTTCACCTTGTGGGTTCT+GGG + chr1_3:38511887-38511906 Msa0117520:intron 50.0%
GTTTCACCTTGTGGGTTCTG+GGG + chr1_3:38511888-38511907 Msa0117520:intron 50.0%
ACATGCATGACACCGTCTCT+CGG - chr1_3:38511953-38511972 None:intergenic 50.0%
ACCAGGATCTGCCTCATCAT+TGG - chr1_3:38513426-38513445 None:intergenic 50.0%
AATGATGAGGCAGATCCTGG+TGG + chr1_3:38513425-38513444 Msa0117520:CDS 50.0%
GAAAAGAAGGGTGGTTGCTG+CGG + chr1_3:38513509-38513528 Msa0117520:CDS 50.0%
GGGAAGCTTATTGCTCACTC+TGG + chr1_3:38513564-38513583 Msa0117520:three_prime_UTR 50.0%
GGGACACTGCTGGTCAAGAA+AGG + chr1_3:38510875-38510894 Msa0117520:CDS 55.0%
! TTTGATGCCCGTGTCGGTGT+CGG + chr1_3:38511665-38511684 Msa0117520:intron 55.0%
TTATGGAGCCGACACCGACA+CGG - chr1_3:38511676-38511695 None:intergenic 55.0%
! TGGGTGCTTTGGGTAAAGGG+TGG + chr1_3:38511851-38511870 Msa0117520:intron 55.0%
! GGGTGCTTTGGGTAAAGGGT+GGG + chr1_3:38511852-38511871 Msa0117520:intron 55.0%
GCGTATTACCGTGGAGCTGT+TGG + chr1_3:38513110-38513129 Msa0117520:CDS 55.0%
ACATCGCTCCAACAGCTCCA+CGG - chr1_3:38513121-38513140 None:intergenic 55.0%
GCCAATGATGAGGCAGATCC+TGG + chr1_3:38513422-38513441 Msa0117520:CDS 55.0%
!! CAGATCCTGGTGGTGGTTCA+GGG + chr1_3:38513435-38513454 Msa0117520:CDS 55.0%
TGGTGGTTCAGGGCTTCTGA+AGG + chr1_3:38513445-38513464 Msa0117520:CDS 55.0%
GGTGGTTCAGGGCTTCTGAA+GGG + chr1_3:38513446-38513465 Msa0117520:CDS 55.0%
!! ACTCTGGCTCTGCCTACATG+TGG + chr1_3:38513580-38513599 Msa0117520:three_prime_UTR 55.0%
!! CTCTGGCTCTGCCTACATGT+GGG + chr1_3:38513581-38513600 Msa0117520:three_prime_UTR 55.0%
TATGGAGCCGACACCGACAC+GGG - chr1_3:38511675-38511694 None:intergenic 60.0%
!! TCACCTTGTGGGTTCTGGGG+AGG + chr1_3:38511891-38511910 Msa0117520:intron 60.0%
!! CACCTTGTGGGTTCTGGGGA+GGG + chr1_3:38511892-38511911 Msa0117520:intron 60.0%
TACCCTCCCCAGAACCCACA+AGG - chr1_3:38511897-38511916 None:intergenic 60.0%
TACCCCCACAAGCCGAGAGA+CGG + chr1_3:38511938-38511957 Msa0117520:intron 60.0%
! ACACCGTCTCTCGGCTTGTG+GGG - chr1_3:38511944-38511963 None:intergenic 60.0%
! GACACCGTCTCTCGGCTTGT+GGG - chr1_3:38511945-38511964 None:intergenic 60.0%
! TGACACCGTCTCTCGGCTTG+TGG - chr1_3:38511946-38511965 None:intergenic 60.0%
GATGAGGCAGATCCTGGTGG+TGG + chr1_3:38513428-38513447 Msa0117520:CDS 60.0%
!! GCAGATCCTGGTGGTGGTTC+AGG + chr1_3:38513434-38513453 Msa0117520:CDS 60.0%
AGAAGCCCTGAACCACCACC+AGG - chr1_3:38513443-38513462 None:intergenic 60.0%
! CAGGGCTTCTGAAGGGAACC+AGG + chr1_3:38513453-38513472 Msa0117520:CDS 60.0%
GGCTCTGCCTACATGTGGGT+TGG + chr1_3:38513585-38513604 Msa0117520:three_prime_UTR 60.0%
GAGTTCCGGGGTTCGAACCC+CGG - chr1_3:38511273-38511292 None:intergenic 65.0%
! CACCGTCTCTCGGCTTGTGG+GGG - chr1_3:38511943-38511962 None:intergenic 65.0%
GATGGCCGGGGTTCGAACCC+CGG + chr1_3:38511265-38511284 Msa0117520:intron 70.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 38510318 38513868 38510318 ID=Msa0117520;Name=Msa0117520
chr1_3 mRNA 38510318 38513868 38510318 ID=Msa0117520-mRNA-1;Parent=Msa0117520;Name=Msa0117520-mRNA-1;_AED=0.04;_eAED=0.04;_QI=352|1|1|1|1|1|2|333|223
chr1_3 exon 38510318 38510896 38510318 ID=Msa0117520-mRNA-1:exon:12049;Parent=Msa0117520-mRNA-1
chr1_3 exon 38513091 38513868 38513091 ID=Msa0117520-mRNA-1:exon:12050;Parent=Msa0117520-mRNA-1
chr1_3 five_prime_UTR 38510318 38510669 38510318 ID=Msa0117520-mRNA-1:five_prime_utr;Parent=Msa0117520-mRNA-1
chr1_3 CDS 38510670 38510896 38510670 ID=Msa0117520-mRNA-1:cds;Parent=Msa0117520-mRNA-1
chr1_3 CDS 38513091 38513535 38513091 ID=Msa0117520-mRNA-1:cds;Parent=Msa0117520-mRNA-1
chr1_3 three_prime_UTR 38513536 38513868 38513536 ID=Msa0117520-mRNA-1:three_prime_utr;Parent=Msa0117520-mRNA-1
Gene Sequence

>Msa0117520

ATGTCGAATTTATACGGTGATTATAATCAGAAGATCGATTACGTTTTCAAAGTGGTGTTGATTGGTGATTCTGCCGTTGGAAAAACACAACTTCTTGCTCGTTTTTCGAGGAATCAATTTAACGTTGATTCTAAAGCTACAATTGGTGTTGAGTTTCAAACCAAAACTCTTGTTATTGATAATAAAACTGTTAAGGCTCAAATATGGGACACTGCTGGTCAAGAAAGGTACAGAGCAGTAACTAGTGCGTATTACCGTGGAGCTGTTGGAGCGATGTTAGTTTACGACATGACTAAGCGTCAATCATTTGATCACATGGCAAGATGGTTAGAGGAACTGAGGGGTCATGCAGACACAAATATTGTCATAATGCTAATTGGCAACAAGTGTGATTTGGGAACTCTAAAGGCAGTACCTACTGAAGATGCTCAGGAGTTTGCACAAAGAGAGAACCTTTTCTTTATGGAGACATCAGCACTAGAGTCCACTAATGTCGAAACCGCCTTTCTGACTATTCTAACAGAAATTTACCGACTTATCAGCAAAAAAACACTTACTGCCAATGATGAGGCAGATCCTGGTGGTGGTTCAGGGCTTCTGAAGGGAACCAGGATAATTGTTCCTAACCAAGATGCGAGTGCTTTTGAAAAGAAGGGTGGTTGCTGCGGATAA
Protein sequence

>Msa0117520

MSNLYGDYNQKIDYVFKVVLIGDSAVGKTQLLARFSRNQFNVDSKATIGVEFQTKTLVIDNKTVKAQIWDTAGQERYRAVTSAYYRGAVGAMLVYDMTKRQSFDHMARWLEELRGHADTNIVIMLIGNKCDLGTLKAVPTEDAQEFAQRENLFFMETSALESTNVETAFLTILTEIYRLISKKTLTANDEADPGGGSGLLKGTRIIVPNQDASAFEKKGGCCG*