AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0197850

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0197850 MtrunA17_Chr2g0290651 96.918 584 15 1 1 581 1 584 0.0 1097
Msa0197850 MtrunA17_Chr4g0076431 59.370 571 207 7 11 578 2 550 0.0 556
Msa0197850 MtrunA17_Chr3g0102681 43.186 565 309 7 8 565 11 570 1.39e-106 334
Msa0197850 MtrunA17_Chr3g0078801 39.506 567 330 7 9 565 12 575 1.62e-89 290
Msa0197850 MtrunA17_Chr5g0446731 38.624 567 335 7 9 565 12 575 4.00e-89 288
Msa0197850 MtrunA17_Chr4g0075951 53.292 319 137 4 262 578 6 314 5.11e-79 252
Msa0197850 MtrunA17_Chr3g0133891 28.548 613 356 16 1 562 270 851 3.26e-29 123
Msa0197850 MtrunA17_Chr1g0153431 26.833 600 365 18 14 563 259 834 1.26e-23 105
Msa0197850 MtrunA17_Chr7g0244501 27.018 285 205 2 282 565 150 432 8.21e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0197850 AT1G12150.2 45.357 560 275 8 11 563 8 543 5.59e-127 384
Msa0197850 AT1G12150.1 45.357 560 275 8 11 563 8 543 5.59e-127 384
Msa0197850 AT5G55860.1 41.901 568 315 6 6 565 8 568 4.64e-86 281
Msa0197850 AT4G17210.1 34.220 564 312 7 10 563 12 526 4.25e-68 230
Msa0197850 AT2G26570.1 26.514 611 380 15 8 575 153 737 3.62e-27 117
Msa0197850 AT2G26570.2 26.514 611 380 15 8 575 153 737 3.62e-27 117
Msa0197850 AT4G33390.1 28.088 591 375 16 8 561 138 715 9.67e-24 106
Msa0197850 AT5G42880.1 25.251 598 362 18 6 565 115 665 2.80e-13 73.6
Msa0197850 AT1G45545.2 24.370 595 348 17 8 562 143 675 3.03e-13 73.2
Msa0197850 AT1G45545.1 24.370 595 348 17 8 562 142 674 3.39e-13 73.2

Find 95 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTCTCTTGATGCTTTCA+TGG 0.211121 2_1:-16186589 None:intergenic
AGCTAAAACTTGTCTGTTAA+AGG 0.228348 2_1:-16187931 None:intergenic
TCGAGACTTTGTGTTTCTTC+AGG 0.293848 2_1:-16186747 None:intergenic
TGAAACAAGTGCTGAAATTC+AGG 0.294476 2_1:+16186782 Msa0197850:CDS
CAATCTGTCAAGGCTGCTAT+TGG 0.319700 2_1:+16185440 Msa0197850:CDS
CAAGGAAACACCTTAAAATA+TGG 0.325427 2_1:-16185060 None:intergenic
TGCCGCTTTGGAGGCGAAAT+TGG 0.336907 2_1:+16186482 Msa0197850:CDS
TTCTGTCGAGTTAATAACTT+TGG 0.337764 2_1:+16186848 Msa0197850:CDS
GACAAACTTTCATATTGAAC+TGG 0.338022 2_1:-16187842 None:intergenic
TTGGAGAACTATCTCTGTTT+CGG 0.342248 2_1:-16185364 None:intergenic
CAATTCTTGAAGTGTTATAT+CGG 0.350713 2_1:-16186235 None:intergenic
TTGGTGAAGTTCGTGTCAAC+CGG 0.365739 2_1:+16185468 Msa0197850:CDS
AATAAAAGCAGCAACAGATT+TGG 0.368777 2_1:+16187535 Msa0197850:CDS
AAGGCTGCTATTGGTTTGTT+TGG 0.369565 2_1:+16185449 Msa0197850:CDS
CAAAGAAAAGGAAGATTCTA+TGG 0.384617 2_1:+16187052 Msa0197850:CDS
ACCGGAGAATCTGTCTCCTT+TGG 0.398317 2_1:-16185383 None:intergenic
CAAAGAAAATTCTCATCAAA+AGG 0.404835 2_1:+16185172 Msa0197850:five_prime_UTR
TATTTCTTCAGACAAAGCTT+TGG 0.407479 2_1:-16187216 None:intergenic
TGAGAGTCATTGTCAAGAAA+TGG 0.411564 2_1:+16187091 Msa0197850:CDS
GAAAGAACAAGAGGAACTTA+AGG 0.412694 2_1:+16186971 Msa0197850:CDS
TTCTAGCTCTGCAGCGAGAT+TGG 0.424207 2_1:-16187671 None:intergenic
AGGAACCAAAGTTTACATCT+TGG 0.434259 2_1:+16185126 Msa0197850:five_prime_UTR
CATGCTCATTGTCAACATCT+TGG 0.434871 2_1:+16187906 Msa0197850:three_prime_UTR
TGGCGTCAAGAAGAACAAAA+AGG 0.451952 2_1:+16187620 Msa0197850:CDS
AAGAACCAAGATGTAAACTT+TGG 0.460675 2_1:-16185131 None:intergenic
AGAAATAGATAATAAATTGA+TGG 0.464169 2_1:+16186704 Msa0197850:CDS
TGATAGAATTATTTCTATCC+CGG 0.469818 2_1:-16185487 None:intergenic
AGCGCTTATCGAGCTTGATA+AGG 0.471291 2_1:+16186212 Msa0197850:CDS
TACCTTGTAGTTCGCCGGTT+AGG 0.471965 2_1:-16187028 None:intergenic
AGAGATGAGAATGAAAGCTC+AGG 0.473161 2_1:+16187169 Msa0197850:CDS
GTGTGATATGCAGAATGTAT+TGG 0.475004 2_1:+16186104 Msa0197850:intron
AAAGGAGACTCAGCTTCTCT+TGG 0.484746 2_1:+16186128 Msa0197850:CDS
TATGCAGAATGTATTGGAAA+AGG 0.485982 2_1:+16186110 Msa0197850:intron
GGCAGGAGAAGCTCAGCGTT+CGG 0.487792 2_1:+16186518 Msa0197850:CDS
GCTCTTGCTGCAAACCTAAC+CGG 0.490559 2_1:+16187014 Msa0197850:CDS
AAGCGATAGGGTATGAAGCT+TGG 0.500293 2_1:+16186361 Msa0197850:CDS
AGAACTAGAACATGCAAGAA+AGG 0.501099 2_1:+16186389 Msa0197850:CDS
AGATTCTCCGGTGGGAGAGA+GGG 0.502913 2_1:+16185394 Msa0197850:CDS
TCATATTGAACTGGGAGAGT+TGG 0.507578 2_1:-16187833 None:intergenic
TTGGCTGCATTTCAGATGGC+AGG 0.508839 2_1:+16186501 Msa0197850:CDS
AATCACTTTGGCTGTAGCGT+TGG 0.509542 2_1:+16187749 Msa0197850:three_prime_UTR
TCAGCAAAACTAGCAGTTGA+AGG 0.511221 2_1:+16187584 Msa0197850:CDS
ACTACAAGGTAGCAAAGAAA+AGG 0.511736 2_1:+16187040 Msa0197850:CDS
GAATGGATTGAGTGAAGTCT+CGG 0.518504 2_1:+16187715 Msa0197850:three_prime_UTR
AGAGTTTCGTAATTCTGAGA+TGG 0.518540 2_1:+16186824 Msa0197850:CDS
TCCAAAGGAGACAGATTCTC+CGG 0.522645 2_1:+16185382 Msa0197850:CDS
TTTGCTACCTTGTAGTTCGC+CGG 0.522771 2_1:-16187033 None:intergenic
AGAAACAACTGCAATGGCTC+AGG 0.523434 2_1:+16187442 Msa0197850:CDS
GATGAATTTGCTGCTCTAAG+TGG 0.529272 2_1:+16187383 Msa0197850:CDS
CAGATTCTCCGGTGGGAGAG+AGG 0.533950 2_1:+16185393 Msa0197850:CDS
AAAGATCAATGAACTTTCAA+AGG 0.534366 2_1:+16186557 Msa0197850:CDS
GGCTCTGAGGAATGCAGAGA+TGG 0.535386 2_1:+16187556 Msa0197850:CDS
AGAAGAACAGATTAAAGCGA+TGG 0.537448 2_1:+16186638 Msa0197850:CDS
TCAAGCCAAAGAACTCGAAA+AGG 0.537588 2_1:+16186326 Msa0197850:CDS
GCTTTGTCTGAAGAAATAGA+AGG 0.539516 2_1:+16187221 Msa0197850:CDS
GAAACACCTTAAAATATGGT+TGG 0.541581 2_1:-16185056 None:intergenic
GCAGCCTTGACAGATTGAAA+TGG 0.548681 2_1:-16185434 None:intergenic
AACAACTGCAATGGCTCAGG+TGG 0.549356 2_1:+16187445 Msa0197850:CDS
ACAAGTGAAGAAAGAACAAG+AGG 0.551605 2_1:+16186962 Msa0197850:CDS
GCGCTATCACAGAAAGCGAT+AGG 0.554886 2_1:+16186348 Msa0197850:CDS
CGCTATCACAGAAAGCGATA+GGG 0.555926 2_1:+16186349 Msa0197850:CDS
GGTCCATCACAAAATCACTT+TGG 0.556573 2_1:+16187737 Msa0197850:three_prime_UTR
GAAATTGGCTGCATTTCAGA+TGG 0.565691 2_1:+16186497 Msa0197850:CDS
TCAATCTCCCTCTCTCCCAC+CGG 0.566121 2_1:-16185401 None:intergenic
AGAATCAAAGCAATCTGCAA+TGG 0.566703 2_1:+16186281 Msa0197850:CDS
GAAGAACAGATTAAAGCGAT+GGG 0.567926 2_1:+16186639 Msa0197850:CDS
AGCTTCACTGAGAAACCTTG+TGG 0.568186 2_1:+16186917 Msa0197850:CDS
CTTACCACTGGAATCAGAAA+CGG 0.572360 2_1:-16187345 None:intergenic
AACCTAACCGGCGAACTACA+AGG 0.573065 2_1:+16187026 Msa0197850:CDS
TGGTGAAGTTCGTGTCAACC+GGG 0.573487 2_1:+16185469 Msa0197850:CDS
ACAAGAGGAACTTAAGGAGA+AGG 0.576875 2_1:+16186977 Msa0197850:CDS
CTGTCTTAAGCGAATCCACA+AGG 0.577030 2_1:-16186932 None:intergenic
TGTCAACACGATCTTACCAC+TGG 0.594761 2_1:-16187357 None:intergenic
ACAAACTTTCATATTGAACT+GGG 0.599851 2_1:-16187841 None:intergenic
AATGTGAAGATTTGATTGAG+AGG 0.600637 2_1:+16187417 Msa0197850:CDS
AATGGATTGAGTGAAGTCTC+GGG 0.603563 2_1:+16187716 Msa0197850:three_prime_UTR
TCTTTATCTTGAGAGTTGAG+AGG 0.603983 2_1:+16187780 Msa0197850:three_prime_UTR
ATCTGCAATGGAAGAAAGTG+AGG 0.605339 2_1:+16186293 Msa0197850:CDS
AAATAAAAGTTAGACTTGCA+AGG 0.613854 2_1:+16185106 Msa0197850:five_prime_UTR
CAGCAACAGATTTGGCTCTG+AGG 0.613910 2_1:+16187543 Msa0197850:CDS
AGCACCATTTCAATCTGTCA+AGG 0.619152 2_1:+16185430 Msa0197850:CDS
CAAGCAGTTATCATTCGAGA+CGG 0.628969 2_1:+16187124 Msa0197850:CDS
GCGTTCGGCGAAATTGAACT+CGG 0.630622 2_1:+16186533 Msa0197850:CDS
AAGGAGACAGATTCTCCGGT+GGG 0.630732 2_1:+16185386 Msa0197850:CDS
TATCAAGAGAAGATCTTCAG+AGG 0.632667 2_1:+16185505 Msa0197850:CDS
AAACAGAGATAGTTCTCCAA+AGG 0.633433 2_1:+16185367 Msa0197850:CDS
GAGGACAGAAACAACTGCAA+TGG 0.637220 2_1:+16187436 Msa0197850:CDS
GTCTCCGTTTCTGATTCCAG+TGG 0.639257 2_1:+16187341 Msa0197850:CDS
TTGAAGGTGAATTAAAGAAG+TGG 0.644395 2_1:+16187600 Msa0197850:CDS
TAAGGAGAAGGAACAAGCAG+CGG 0.645739 2_1:+16186989 Msa0197850:CDS
AGCCAATTTCGCCTCCAAAG+CGG 0.663081 2_1:-16186484 None:intergenic
AAAGGAGACAGATTCTCCGG+TGG 0.663875 2_1:+16185385 Msa0197850:CDS
CACATCTGCACAATGTTGTG+TGG 0.666126 2_1:-16187877 None:intergenic
TGAAGAAACACAAAGTCTCG+AGG 0.675914 2_1:+16186749 Msa0197850:CDS
AAGTTGTTGTCTGACGCACA+AGG 0.705883 2_1:+16187314 Msa0197850:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAGATTATATACTATTTTT+AGG - chr2_1:16185010-16185029 None:intergenic 10.0%
!! TTATAAAATATTATGTAACA+CGG + chr2_1:16185264-16185283 Msa0197850:intron 10.0%
!! GGTACAAAAAAAAAAAAAAA+AGG - chr2_1:16185580-16185599 None:intergenic 15.0%
!!! TTTTTTTTTTTTTTTGTACC+AGG + chr2_1:16185580-16185599 Msa0197850:intron 15.0%
!! AGAAATAGATAATAAATTGA+TGG + chr2_1:16186704-16186723 Msa0197850:CDS 15.0%
!!! ATTTAACCAACCATATTTTA+AGG + chr2_1:16185050-16185069 Msa0197850:five_prime_UTR 20.0%
!! AACGATTATTTGCAATATTA+AGG - chr2_1:16185550-16185569 None:intergenic 20.0%
!! TTAATATTGCAAATAATCGT+TGG + chr2_1:16185549-16185568 Msa0197850:intron 20.0%
!! ATTTGTAATATTAAGGATTG+CGG - chr2_1:16185619-16185638 None:intergenic 20.0%
!! CATGGTTATTTGTAATATTA+AGG - chr2_1:16185626-16185645 None:intergenic 20.0%
!! CTTAATATTACAAATAACCA+TGG + chr2_1:16185624-16185643 Msa0197850:intron 20.0%
!!! AAACTTTTACAAAACTTCAA+AGG + chr2_1:16186680-16186699 Msa0197850:CDS 20.0%
!!! ACTATTTTTAGGTTGAGAAA+TGG - chr2_1:16184999-16185018 None:intergenic 25.0%
! AAATAAAAGTTAGACTTGCA+AGG + chr2_1:16185106-16185125 Msa0197850:five_prime_UTR 25.0%
!! TGATGAGAATTTTCTTTGAA+AGG - chr2_1:16185170-16185189 None:intergenic 25.0%
! CAAAGAAAATTCTCATCAAA+AGG + chr2_1:16185172-16185191 Msa0197850:five_prime_UTR 25.0%
!! TTTTGTAGCATCAAACATTA+AGG + chr2_1:16185199-16185218 Msa0197850:five_prime_UTR 25.0%
! ATTGCATAACATGTTACATA+TGG - chr2_1:16185245-16185264 None:intergenic 25.0%
!!! TGATAGAATTATTTCTATCC+CGG - chr2_1:16185490-16185509 None:intergenic 25.0%
! ATTTGCAATATTAAGGATAG+CGG - chr2_1:16185543-16185562 None:intergenic 25.0%
! TTATTCGCAAAATTTAACCA+TGG - chr2_1:16185644-16185663 None:intergenic 25.0%
!! AATAGCAGTTTGTTCAAATT+CGG + chr2_1:16185864-16185883 Msa0197850:intron 25.0%
!!! AATGCCAATTTTAATACAAG+AGG + chr2_1:16185965-16185984 Msa0197850:intron 25.0%
!! CAATTCTTGAAGTGTTATAT+CGG - chr2_1:16186238-16186257 None:intergenic 25.0%
!! TATTTTGTTAAGTTCTTGCT+TGG - chr2_1:16186448-16186467 None:intergenic 25.0%
! AAAGATCAATGAACTTTCAA+AGG + chr2_1:16186557-16186576 Msa0197850:CDS 25.0%
! ACAAACTTTCATATTGAACT+GGG - chr2_1:16187844-16187863 None:intergenic 25.0%
! TTTTTAACAACACTTGAGGT+TGG + chr2_1:16184941-16184960 Msa0197850:five_prime_UTR 30.0%
GAAACACCTTAAAATATGGT+TGG - chr2_1:16185059-16185078 None:intergenic 30.0%
CAAGGAAACACCTTAAAATA+TGG - chr2_1:16185063-16185082 None:intergenic 30.0%
AAGAACCAAGATGTAAACTT+TGG - chr2_1:16185134-16185153 None:intergenic 30.0%
!! GAATTTTCTTTGAAAGGTGT+TGG - chr2_1:16185164-16185183 None:intergenic 30.0%
TGTGCCTCTTGTATTAAAAT+TGG - chr2_1:16185972-16185991 None:intergenic 30.0%
!!! TTTTCAAGTTTGCGTGATTT+TGG + chr2_1:16186007-16186026 Msa0197850:intron 30.0%
TATTTATGAGTTATGCGTCA+AGG + chr2_1:16186070-16186089 Msa0197850:intron 30.0%
TATGCAGAATGTATTGGAAA+AGG + chr2_1:16186110-16186129 Msa0197850:intron 30.0%
!!! ATCTAGTTCTTTTACAGTTG+TGG - chr2_1:16186421-16186440 None:intergenic 30.0%
TTCTGTCGAGTTAATAACTT+TGG + chr2_1:16186848-16186867 Msa0197850:CDS 30.0%
CAAAGAAAAGGAAGATTCTA+TGG + chr2_1:16187052-16187071 Msa0197850:CDS 30.0%
TATTTCTTCAGACAAAGCTT+TGG - chr2_1:16187219-16187238 None:intergenic 30.0%
AATGTGAAGATTTGATTGAG+AGG + chr2_1:16187417-16187436 Msa0197850:CDS 30.0%
AATAAAAGCAGCAACAGATT+TGG + chr2_1:16187535-16187554 Msa0197850:CDS 30.0%
TTGAAGGTGAATTAAAGAAG+TGG + chr2_1:16187600-16187619 Msa0197850:CDS 30.0%
TCAAGAAGAACAAAAAGGTT+TGG + chr2_1:16187625-16187644 Msa0197850:CDS 30.0%
ACAAAAAGGTTTGGATTTGT+CGG + chr2_1:16187634-16187653 Msa0197850:CDS 30.0%
! TAAGTCTTTTAAGCTCAGAA+TGG + chr2_1:16187698-16187717 Msa0197850:three_prime_UTR 30.0%
GACAAACTTTCATATTGAAC+TGG - chr2_1:16187845-16187864 None:intergenic 30.0%
AGCTAAAACTTGTCTGTTAA+AGG - chr2_1:16187934-16187953 None:intergenic 30.0%
! GCTTTTACATCTCAATATGT+TGG - chr2_1:16187983-16188002 None:intergenic 30.0%
!! AAATGGATAGAGTTTTGTGG+TGG - chr2_1:16184982-16185001 None:intergenic 35.0%
! GAGAAATGGATAGAGTTTTG+TGG - chr2_1:16184985-16185004 None:intergenic 35.0%
!!! GGTGTTTCCTTGTTTTTCAA+CGG + chr2_1:16185071-16185090 Msa0197850:five_prime_UTR 35.0%
! TTTGAGACCGTTGAAAAACA+AGG - chr2_1:16185081-16185100 None:intergenic 35.0%
AGGAACCAAAGTTTACATCT+TGG + chr2_1:16185126-16185145 Msa0197850:five_prime_UTR 35.0%
TTGGAGAACTATCTCTGTTT+CGG - chr2_1:16185367-16185386 None:intergenic 35.0%
AAACAGAGATAGTTCTCCAA+AGG + chr2_1:16185367-16185386 Msa0197850:CDS 35.0%
TATCAAGAGAAGATCTTCAG+AGG + chr2_1:16185505-16185524 Msa0197850:CDS 35.0%
ATCTTCAGAGGTAAACATCA+CGG + chr2_1:16185517-16185536 Msa0197850:intron 35.0%
! TTTTGACGTCTATGCGATTA+AGG - chr2_1:16185683-16185702 None:intergenic 35.0%
TGCAATATAGCACTATAGCA+TGG - chr2_1:16185890-16185909 None:intergenic 35.0%
GTGTGATATGCAGAATGTAT+TGG + chr2_1:16186104-16186123 Msa0197850:intron 35.0%
AGAATCAAAGCAATCTGCAA+TGG + chr2_1:16186281-16186300 Msa0197850:CDS 35.0%
AGAACTAGAACATGCAAGAA+AGG + chr2_1:16186389-16186408 Msa0197850:CDS 35.0%
TTCTTCTCTTGATGCTTTCA+TGG - chr2_1:16186592-16186611 None:intergenic 35.0%
AGAAGAACAGATTAAAGCGA+TGG + chr2_1:16186638-16186657 Msa0197850:CDS 35.0%
GAAGAACAGATTAAAGCGAT+GGG + chr2_1:16186639-16186658 Msa0197850:CDS 35.0%
!! TGAAACAAGTGCTGAAATTC+AGG + chr2_1:16186782-16186801 Msa0197850:CDS 35.0%
! AGAGTTTCGTAATTCTGAGA+TGG + chr2_1:16186824-16186843 Msa0197850:CDS 35.0%
ACAAGTGAAGAAAGAACAAG+AGG + chr2_1:16186962-16186981 Msa0197850:CDS 35.0%
GAAAGAACAAGAGGAACTTA+AGG + chr2_1:16186971-16186990 Msa0197850:CDS 35.0%
ACTACAAGGTAGCAAAGAAA+AGG + chr2_1:16187040-16187059 Msa0197850:CDS 35.0%
TGAGAGTCATTGTCAAGAAA+TGG + chr2_1:16187091-16187110 Msa0197850:CDS 35.0%
GCTTTGTCTGAAGAAATAGA+AGG + chr2_1:16187221-16187240 Msa0197850:CDS 35.0%
AGATGAAAAAAGAGCTGTTG+AGG + chr2_1:16187286-16187305 Msa0197850:CDS 35.0%
TCTTTATCTTGAGAGTTGAG+AGG + chr2_1:16187780-16187799 Msa0197850:three_prime_UTR 35.0%
AGCACCATTTCAATCTGTCA+AGG + chr2_1:16185430-16185449 Msa0197850:CDS 40.0%
!! AAGGCTGCTATTGGTTTGTT+TGG + chr2_1:16185449-16185468 Msa0197850:CDS 40.0%
GAAGCAGGGTTATGACAAAA+TGG - chr2_1:16185747-16185766 None:intergenic 40.0%
AGCATTCAATCTTCGAAGCA+GGG - chr2_1:16185761-16185780 None:intergenic 40.0%
AAGCATTCAATCTTCGAAGC+AGG - chr2_1:16185762-16185781 None:intergenic 40.0%
TGCTTCGAAGATTGAATGCT+TGG + chr2_1:16185761-16185780 Msa0197850:intron 40.0%
CATTGCACAACAATAGCGAT+TGG + chr2_1:16185908-16185927 Msa0197850:intron 40.0%
ATCTGCAATGGAAGAAAGTG+AGG + chr2_1:16186293-16186312 Msa0197850:CDS 40.0%
TCAAGCCAAAGAACTCGAAA+AGG + chr2_1:16186326-16186345 Msa0197850:CDS 40.0%
!! ACAAGATTTTGATGCCGCTT+TGG + chr2_1:16186470-16186489 Msa0197850:CDS 40.0%
GAAATTGGCTGCATTTCAGA+TGG + chr2_1:16186497-16186516 Msa0197850:CDS 40.0%
TCGAGACTTTGTGTTTCTTC+AGG - chr2_1:16186750-16186769 None:intergenic 40.0%
TGAAGAAACACAAAGTCTCG+AGG + chr2_1:16186749-16186768 Msa0197850:CDS 40.0%
ACAAGAGGAACTTAAGGAGA+AGG + chr2_1:16186977-16186996 Msa0197850:CDS 40.0%
CAAGCAGTTATCATTCGAGA+CGG + chr2_1:16187124-16187143 Msa0197850:CDS 40.0%
AGAGATGAGAATGAAAGCTC+AGG + chr2_1:16187169-16187188 Msa0197850:CDS 40.0%
CTTACCACTGGAATCAGAAA+CGG - chr2_1:16187348-16187367 None:intergenic 40.0%
!! GATGAATTTGCTGCTCTAAG+TGG + chr2_1:16187383-16187402 Msa0197850:CDS 40.0%
TCAGCAAAACTAGCAGTTGA+AGG + chr2_1:16187584-16187603 Msa0197850:CDS 40.0%
TGGCGTCAAGAAGAACAAAA+AGG + chr2_1:16187620-16187639 Msa0197850:CDS 40.0%
!! GAATGGATTGAGTGAAGTCT+CGG + chr2_1:16187715-16187734 Msa0197850:three_prime_UTR 40.0%
!! AATGGATTGAGTGAAGTCTC+GGG + chr2_1:16187716-16187735 Msa0197850:three_prime_UTR 40.0%
GGTCCATCACAAAATCACTT+TGG + chr2_1:16187737-16187756 Msa0197850:three_prime_UTR 40.0%
! CAGCCAAAGTGATTTTGTGA+TGG - chr2_1:16187743-16187762 None:intergenic 40.0%
TCATATTGAACTGGGAGAGT+TGG - chr2_1:16187836-16187855 None:intergenic 40.0%
CATGCTCATTGTCAACATCT+TGG + chr2_1:16187906-16187925 Msa0197850:three_prime_UTR 40.0%
TCCAAAGGAGACAGATTCTC+CGG + chr2_1:16185382-16185401 Msa0197850:CDS 45.0%
GCAGCCTTGACAGATTGAAA+TGG - chr2_1:16185437-16185456 None:intergenic 45.0%
CAATCTGTCAAGGCTGCTAT+TGG + chr2_1:16185440-16185459 Msa0197850:CDS 45.0%
TTGGTGAAGTTCGTGTCAAC+CGG + chr2_1:16185468-16185487 Msa0197850:CDS 45.0%
TTGTACCAGGAAGCAGATCA+TGG + chr2_1:16185593-16185612 Msa0197850:intron 45.0%
CGTCAAAAACCTTGATGTCG+CGG + chr2_1:16185695-16185714 Msa0197850:intron 45.0%
!! CAGTTTTAACCGCGACATCA+AGG - chr2_1:16185707-16185726 None:intergenic 45.0%
GATTGAATGCTTGGTCCCAA+AGG + chr2_1:16185770-16185789 Msa0197850:intron 45.0%
CGACTAACTAGCTACCATCT+GGG - chr2_1:16185801-16185820 None:intergenic 45.0%
AAAGGAGACTCAGCTTCTCT+TGG + chr2_1:16186128-16186147 Msa0197850:CDS 45.0%
AGCGCTTATCGAGCTTGATA+AGG + chr2_1:16186212-16186231 Msa0197850:CDS 45.0%
!!! TAGCGCCTTTTCGAGTTCTT+TGG - chr2_1:16186334-16186353 None:intergenic 45.0%
CGCTATCACAGAAAGCGATA+GGG + chr2_1:16186349-16186368 Msa0197850:CDS 45.0%
AAGCGATAGGGTATGAAGCT+TGG + chr2_1:16186361-16186380 Msa0197850:CDS 45.0%
!!! AGATTTTGATGCCGCTTTGG+AGG + chr2_1:16186473-16186492 Msa0197850:CDS 45.0%
AGCTTCACTGAGAAACCTTG+TGG + chr2_1:16186917-16186936 Msa0197850:CDS 45.0%
CTGTCTTAAGCGAATCCACA+AGG - chr2_1:16186935-16186954 None:intergenic 45.0%
TAAGGAGAAGGAACAAGCAG+CGG + chr2_1:16186989-16187008 Msa0197850:CDS 45.0%
TTTGCTACCTTGTAGTTCGC+CGG - chr2_1:16187036-16187055 None:intergenic 45.0%
AAGTTGTTGTCTGACGCACA+AGG + chr2_1:16187314-16187333 Msa0197850:CDS 45.0%
TGTCAACACGATCTTACCAC+TGG - chr2_1:16187360-16187379 None:intergenic 45.0%
GAGGACAGAAACAACTGCAA+TGG + chr2_1:16187436-16187455 Msa0197850:CDS 45.0%
AGAAACAACTGCAATGGCTC+AGG + chr2_1:16187442-16187461 Msa0197850:CDS 45.0%
AATCACTTTGGCTGTAGCGT+TGG + chr2_1:16187749-16187768 Msa0197850:three_prime_UTR 45.0%
CACATCTGCACAATGTTGTG+TGG - chr2_1:16187880-16187899 None:intergenic 45.0%
ACCGGAGAATCTGTCTCCTT+TGG - chr2_1:16185386-16185405 None:intergenic 50.0%
AAAGGAGACAGATTCTCCGG+TGG + chr2_1:16185385-16185404 Msa0197850:CDS 50.0%
AAGGAGACAGATTCTCCGGT+GGG + chr2_1:16185386-16185405 Msa0197850:CDS 50.0%
TGGTGAAGTTCGTGTCAACC+GGG + chr2_1:16185469-16185488 Msa0197850:CDS 50.0%
ACCATCTGGGTCATGCCTTT+GGG - chr2_1:16185788-16185807 None:intergenic 50.0%
TACCATCTGGGTCATGCCTT+TGG - chr2_1:16185789-16185808 None:intergenic 50.0%
GCGACTAACTAGCTACCATC+TGG - chr2_1:16185802-16185821 None:intergenic 50.0%
GCGCTATCACAGAAAGCGAT+AGG + chr2_1:16186348-16186367 Msa0197850:CDS 50.0%
AGCCAATTTCGCCTCCAAAG+CGG - chr2_1:16186487-16186506 None:intergenic 50.0%
TTGGCTGCATTTCAGATGGC+AGG + chr2_1:16186501-16186520 Msa0197850:CDS 50.0%
GCGTTCGGCGAAATTGAACT+CGG + chr2_1:16186533-16186552 Msa0197850:CDS 50.0%
GCTCTTGCTGCAAACCTAAC+CGG + chr2_1:16187014-16187033 Msa0197850:CDS 50.0%
AACCTAACCGGCGAACTACA+AGG + chr2_1:16187026-16187045 Msa0197850:CDS 50.0%
! TACCTTGTAGTTCGCCGGTT+AGG - chr2_1:16187031-16187050 None:intergenic 50.0%
GTCTCCGTTTCTGATTCCAG+TGG + chr2_1:16187341-16187360 Msa0197850:CDS 50.0%
AACAACTGCAATGGCTCAGG+TGG + chr2_1:16187445-16187464 Msa0197850:CDS 50.0%
!! CAGCAACAGATTTGGCTCTG+AGG + chr2_1:16187543-16187562 Msa0197850:CDS 50.0%
!! TTCTAGCTCTGCAGCGAGAT+TGG - chr2_1:16187674-16187693 None:intergenic 50.0%
AGATTCTCCGGTGGGAGAGA+GGG + chr2_1:16185394-16185413 Msa0197850:CDS 55.0%
TCAATCTCCCTCTCTCCCAC+CGG - chr2_1:16185404-16185423 None:intergenic 55.0%
! TCCCAAAGGCATGACCCAGA+TGG + chr2_1:16185784-16185803 Msa0197850:intron 55.0%
!! TGCCGCTTTGGAGGCGAAAT+TGG + chr2_1:16186482-16186501 Msa0197850:CDS 55.0%
!! GGCTCTGAGGAATGCAGAGA+TGG + chr2_1:16187556-16187575 Msa0197850:CDS 55.0%
CAGATTCTCCGGTGGGAGAG+AGG + chr2_1:16185393-16185412 Msa0197850:CDS 60.0%
TGCGGCCATGATCTGCTTCC+TGG - chr2_1:16185601-16185620 None:intergenic 60.0%
GGCAGGAGAAGCTCAGCGTT+CGG + chr2_1:16186518-16186537 Msa0197850:CDS 60.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 16184939 16188044 16184939 ID=Msa0197850;Name=Msa0197850
chr2_1 mRNA 16184939 16188044 16184939 ID=Msa0197850-mRNA-1;Parent=Msa0197850;Name=Msa0197850-mRNA-1;_AED=0.01;_eAED=0.01;_QI=314|1|1|1|0.5|0.66|3|353|581
chr2_1 exon 16184939 16185220 16184939 ID=Msa0197850-mRNA-1:exon:6673;Parent=Msa0197850-mRNA-1
chr2_1 exon 16185324 16185526 16185324 ID=Msa0197850-mRNA-1:exon:6674;Parent=Msa0197850-mRNA-1
chr2_1 exon 16186117 16188044 16186117 ID=Msa0197850-mRNA-1:exon:6675;Parent=Msa0197850-mRNA-1
chr2_1 five_prime_UTR 16184939 16185220 16184939 ID=Msa0197850-mRNA-1:five_prime_utr;Parent=Msa0197850-mRNA-1
chr2_1 five_prime_UTR 16185324 16185355 16185324 ID=Msa0197850-mRNA-1:five_prime_utr;Parent=Msa0197850-mRNA-1
chr2_1 CDS 16185356 16185526 16185356 ID=Msa0197850-mRNA-1:cds;Parent=Msa0197850-mRNA-1
chr2_1 CDS 16186117 16187691 16186117 ID=Msa0197850-mRNA-1:cds;Parent=Msa0197850-mRNA-1
chr2_1 three_prime_UTR 16187692 16188044 16187692 ID=Msa0197850-mRNA-1:three_prime_utr;Parent=Msa0197850-mRNA-1
Gene Sequence

>Msa0197850

ATGAGTTTCCGAAACAGAGATAGTTCTCCAAAGGAGACAGATTCTCCGGTGGGAGAGAGGGAGATTGACACAAGAGCACCATTTCAATCTGTCAAGGCTGCTATTGGTTTGTTTGGTGAAGTTCGTGTCAACCGGGATAGAAATAATTCTATCAAGAGAAGATCTTCAGAGAATGTATTGGAAAAGGAGACTCAGCTTCTCTTGGCTCAAAGAGAACTGAACAAGATAAAGAAAAAGCTTGAAAGTGCTGAGAGTACAAAAGCTAAAGCGCTTATCGAGCTTGATAAGGCCGATATAACACTTCAAGAATTGACAAAGAAACTCAACACTGTGAGAGAATCAAAGCAATCTGCAATGGAAGAAAGTGAGGTTGTGAAGAATCAAGCCAAAGAACTCGAAAAGGCGCTATCACAGAAAGCGATAGGGTATGAAGCTTGGAAACAAGAACTAGAACATGCAAGAAAGGAGTACACCACAACTGTAAAAGAACTAGATGCTTCCAAGCAAGAACTTAACAAAATAAGACAAGATTTTGATGCCGCTTTGGAGGCGAAATTGGCTGCATTTCAGATGGCAGGAGAAGCTCAGCGTTCGGCGAAATTGAACTCGGAAAAGATCAATGAACTTTCAAAGGAAATTGCAACCATGAAAGCATCAAGAGAAGAACTCAAGCTTGCAACTGCACAAGCACAAGAAGAACAGATTAAAGCGATGGGAGAAAGAGAAGAAAAGTTAAACTTTTACAAAACTTCAAAGGAAGAAATAGATAATAAATTGATGGAAGTGAAGAATGAGTATGATCCTGAAGAAACACAAAGTCTCGAGGCAAAACTTGCTGAAACAAGTGCTGAAATTCAGGTTCTTCAAGAGAAGATAAAAGAGTTTCGTAATTCTGAGATGGATTCTGTCGAGTTAATAACTTTGGAAATCAAAGAAGCAACAAAGACACTGCAAGAAATTTCTGAAGAAGAAGCTTCACTGAGAAACCTTGTGGATTCGCTTAAGACAGAATTAGAACAAGTGAAGAAAGAACAAGAGGAACTTAAGGAGAAGGAACAAGCAGCGGAAGCTCTTGCTGCAAACCTAACCGGCGAACTACAAGGTAGCAAAGAAAAGGAAGATTCTATGGAAAACAAAGAATCTAATGAGAGTCATTGTCAAGAAATGGAGTTGAAAATCAAGCAGTTATCATTCGAGACGGAAAATGCAAGAAAAGAAGAAGAAGAGATGAGAATGAAAGCTCAGGAACTGAAACAAGAAGCTGAGAATTCCAAAGCTTTGTCTGAAGAAATAGAAGGTAAACTAGAGATTTTATTTAAACAAGCTGAAGAAGCAAAAGCAGATGAAAAAAGAGCTGTTGAGGAGATGAAGTTGTTGTCTGACGCACAAGGTAGTGTCTCCGTTTCTGATTCCAGTGGTAAGATCGTGTTGACAGTTGATGAATTTGCTGCTCTAAGTGGAAAAATCAATGAATGTGAAGATTTGATTGAGAGGACAGAAACAACTGCAATGGCTCAGGTGGAAGCAATCAACACAAGAAGAAATGAAGTGAACAAAAAAGTCGAAGCTAATCTTCAAGCAATCGAAGAAATAAAAGCAGCAACAGATTTGGCTCTGAGGAATGCAGAGATGGCAGACTCAGCAAAACTAGCAGTTGAAGGTGAATTAAAGAAGTGGCGTCAAGAAGAACAAAAAGGTTTGGATTTGTCGGATAACTCTTCAACACCAATCTCGCTGCAGAGCTAG
Protein sequence

>Msa0197850

MSFRNRDSSPKETDSPVGEREIDTRAPFQSVKAAIGLFGEVRVNRDRNNSIKRRSSENVLEKETQLLLAQRELNKIKKKLESAESTKAKALIELDKADITLQELTKKLNTVRESKQSAMEESEVVKNQAKELEKALSQKAIGYEAWKQELEHARKEYTTTVKELDASKQELNKIRQDFDAALEAKLAAFQMAGEAQRSAKLNSEKINELSKEIATMKASREELKLATAQAQEEQIKAMGEREEKLNFYKTSKEEIDNKLMEVKNEYDPEETQSLEAKLAETSAEIQVLQEKIKEFRNSEMDSVELITLEIKEATKTLQEISEEEASLRNLVDSLKTELEQVKKEQEELKEKEQAAEALAANLTGELQGSKEKEDSMENKESNESHCQEMELKIKQLSFETENARKEEEEMRMKAQELKQEAENSKALSEEIEGKLEILFKQAEEAKADEKRAVEEMKLLSDAQGSVSVSDSSGKIVLTVDEFAALSGKINECEDLIERTETTAMAQVEAINTRRNEVNKKVEANLQAIEEIKAATDLALRNAEMADSAKLAVEGELKKWRQEEQKGLDLSDNSSTPISLQS*