AlfalfaGEDB | Msa0287310

M. sativa cultivar zhongmu-4 / Msa0287310

KEGG: K17768
GO: -
PFAM: PF07719.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0287310	XP_013463891.1	96.694	726	23	1	1	726	1	725	0.0	1443

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0287310	sp\|F4IRM4\|PHOX1_ARATH	52.710	738	311	12	10	726	20	740	0.0	731

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0287310	A0A072V8J2	96.694	726	23	1	1	726	1	725	0.0	1443

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000550	Msa0287310	-0.800036	1.739886e-48	-8.615850e-47
Msa0004880	Msa0287310	0.801013	1.100190e-48	-8.615850e-47
Msa0004920	Msa0287310	0.813890	2.035910e-51	-8.615850e-47
Msa0006110	Msa0287310	0.800494	1.403532e-48	-8.615850e-47
Msa0006390	Msa0287310	0.801039	1.086871e-48	-8.615850e-47
Msa0008340	Msa0287310	0.805257	1.456853e-49	-8.615850e-47
Msa0008410	Msa0287310	0.803445	3.473741e-49	-8.615850e-47
Msa0015120	Msa0287310	0.831206	1.903295e-55	-8.615850e-47
Msa0015300	Msa0287310	0.808847	2.533208e-50	-8.615850e-47
Msa0017510	Msa0287310	0.802287	6.025409e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0287310	MtrunA17_Chr2g0304261	96.694	726	23	1	1	726	1	725	0.0	1443
Msa0287310	MtrunA17_Chr1g0149561	50.746	737	319	13	1	724	1	706	0.0	704
Msa0287310	MtrunA17_Chr4g0075481	38.174	723	351	11	32	672	31	739	1.03e-145	444
Msa0287310	MtrunA17_Chr2g0289351	41.163	447	222	7	239	677	29	442	1.31e-101	320
Msa0287310	MtrunA17_Chr7g0237741	36.752	117	69	2	37	150	92	206	3.38e-15	76.6
Msa0287310	MtrunA17_Chr2g0323971	34.188	117	72	2	37	150	95	209	6.10e-14	72.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa0287310	AT2G25290.2	52.710	738	311	12	10	726	20	740	731
Msa0287310	AT2G25290.3	52.710	738	311	12	10	726	20	740	731
Msa0287310	AT2G25290.4	52.710	738	311	12	10	726	20	740	731
Msa0287310	AT2G25290.1	52.710	738	311	12	10	726	20	740	731
Msa0287310	AT4G32070.2	50.734	749	309	13	28	726	39	777	709
Msa0287310	AT4G32070.1	50.734	749	309	13	28	726	39	777	709
Msa0287310	AT5G20360.1	47.489	697	337	11	37	722	123	801	600
Msa0287310	AT5G20360.2	47.489	697	337	11	37	722	123	801	600
Msa0287310	AT5G20360.3	47.489	697	337	11	37	722	113	791	599
Msa0287310	AT5G20360.7	47.489	697	337	11	37	722	63	741	599

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Find 203 sgRNAs with CRISPR-Local

Find 454 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTGCACTTGAAGATAG+AGG	0.485144	2_3:-29660249	Msa0287310:CDS
AAAGATGGTTCAACATAATC+CGG	0.460718	2_3:+29660192	None:intergenic
AAAGCGCTCAAATTGCTTCC+GGG	0.441561	2_3:-29660528	Msa0287310:CDS
AAAGTGCACTTGAAGATAGA+GGG	0.624772	2_3:-29660248	Msa0287310:CDS
AAATCCTGATTTGGAGAGAT+GGG	0.510228	2_3:-29661070	Msa0287310:five_prime_UTR
AAATTAAAAGTTCGTATGAA+TGG	0.399777	2_3:-29659237	Msa0287310:CDS
AACAAAGCTTTACCATATTT+TGG	0.130985	2_3:+29660390	None:intergenic
AACATTACAGCTACATCTGT+TGG	0.438941	2_3:+29658746	None:intergenic
AACTGTGAGTGTTGTGAATT+AGG	0.312403	2_3:-29660807	Msa0287310:five_prime_UTR
AAGATGAAGAACCTCTGTGG+AGG	0.649172	2_3:+29659051	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTCAACATTCAAATCTGAGT+TGG	-	chr2_3:29655244-29655263	Msa0287310:three_prime_UTR	30.0%
TCAACATTCAAATCTGAGTT+GGG	-	chr2_3:29655245-29655264	Msa0287310:three_prime_UTR	30.0%
AGAAGAAACAAATCCTGATT+TGG	-	chr2_3:29655304-29655323	Msa0287310:three_prime_UTR	30.0%
TTCTTGAAAACCTTTGTGTT+TGG	-	chr2_3:29655463-29655482	Msa0287310:intron	30.0%
TAGGAGGGTTTGTAATATAT+TGG	-	chr2_3:29655595-29655614	Msa0287310:intron	30.0%
GGGTTTGTAATATATTGGAT+TGG	-	chr2_3:29655600-29655619	Msa0287310:intron	30.0%
TGTGTTTACTATTTGATTGG+TGG	-	chr2_3:29655633-29655652	Msa0287310:intron	30.0%
GTGTTTACTATTTGATTGGT+GGG	-	chr2_3:29655634-29655653	Msa0287310:intron	30.0%
CTTGAAGTTACTCCAAAATA+TGG	-	chr2_3:29655981-29656000	Msa0287310:intron	30.0%
AAAGATGGTTCAACATAATC+CGG	+	chr2_3:29656194-29656213	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_3	gene	29654983	29661422	29654983	ID=Msa0287310;Name=Msa0287310
chr2_3	mRNA	29654983	29661422	29654983	ID=Msa0287310-mRNA-1;Parent=Msa0287310;Name=Msa0287310-mRNA-1;_AED=0.05;_eAED=0.05;_QI=680\|1\|1\|1\|0.5\|0.33\|3\|535\|726
chr2_3	exon	29654983	29655426	29654983	ID=Msa0287310-mRNA-1:exon:10364;Parent=Msa0287310-mRNA-1
chr2_3	exon	29658394	29658633	29658394	ID=Msa0287310-mRNA-1:exon:10363;Parent=Msa0287310-mRNA-1
chr2_3	exon	29658711	29661422	29658711	ID=Msa0287310-mRNA-1:exon:10362;Parent=Msa0287310-mRNA-1
chr2_3	five_prime_UTR	29660743	29661422	29660743	ID=Msa0287310-mRNA-1:five_prime_utr;Parent=Msa0287310-mRNA-1
chr2_3	CDS	29658711	29660742	29658711	ID=Msa0287310-mRNA-1:cds;Parent=Msa0287310-mRNA-1
chr2_3	CDS	29658485	29658633	29658485	ID=Msa0287310-mRNA-1:cds;Parent=Msa0287310-mRNA-1
chr2_3	three_prime_UTR	29658394	29658484	29658394	ID=Msa0287310-mRNA-1:three_prime_utr;Parent=Msa0287310-mRNA-1
chr2_3	three_prime_UTR	29654983	29655426	29654983	ID=Msa0287310-mRNA-1:three_prime_utr;Parent=Msa0287310-mRNA-1

Gene Sequence

>Msa0287310

ATGGGGAAGAAAAAGAAGCAAGTAGGAGAAACAAGTGAGGAAAATGGGAATCATAGTAACGACGGTGATAAAAGTCCGACTGCGTATGATAATGATACTTTGTTGTTTATTGCCACGGCTCAAGAGTTGAAGGAAGAAGGTAATAAGATGTTTCAAAAGAGGGATCTTCGAGGAGCTATGGTAAGGTATGAGAAAGCGCTCAAATTGCTTCCGGGAAATCATATAGATGTGTCGTATGTGCGGAGTAATATGGCTGCGTGCTATATGCAGATGGGACTTGGTGAGTACCCTAGGGCGATTAATGAATGCAATTTGGCTCTTGAAGTTACTCCAAAATATGGTAAAGCTTTGTTGAAGAGAGCGAGGTGTTATGAGGCTTTGAATAGATTGGATTTAGCATTGAGAGATGTTAGTGCTGTTTTGAAGATGGAGCCGAATAATATCATGGCGTTGGAGATCTCGGATAAGGTGAAAAGTGCACTTGAAGATAGAGGGTTAAGAGTAAATGATGAGAAAATTGAGTTGCCTCCGGATTATGTTGAACCATCTTTTGACGTGCCTTCCGAAAAGGCAGTGAAAGAAAAGAAACACAAGAAAAAGAGCAAAAAAGAGAAGGTGAAGGCTTCCGATGAAATTGTAGAGAAGCAAGCCGAGGAGAAACTAGAAGAGAAGAAAGAGGAGGACAACATTGCACCAAATAAGGCTGAGGACGATCATCTCAAAGAGAAAAAGCGTAATAAATCGAAAAAGAAGGCAGCCAAGGAAAAAATTGATAAGCAGAAAGAGGATGTTAAGGAGGTCAATGAAGAAAAAAGTAATGGTAGAAGCGTAGATGTACCTAAGAAAACAGCAAAACTTGTTTTTGGTGATGATATAAGATGGGCTCAATTGCCGGTTAATTGCAGCCTTTTTCAACTAAGAGAAATTATTTGTGACAGGTTCCCAAGCCTTGGAGCAGTTCTTGTCAAATATAGAGATCAAGAAGGTGACTTGATCACAATCACTTCGGATGAAGAATTGAAGTGGGCTGAAACAGGATCACAGGGTTCTATCCGACTGTATATAGTAGAAGCTAATCCTAATCACGATCCATTCTATGAGAAACTTCGTCTGAATGATGTGGAAAAGGTTGAAATGGATAATGCCCCTAAGAACGGTTGTGTGGTGAAATCAAAGGAAATTGTTAGCTCATCTTGTGTTGAGGATTGGATAATTCAGTTCGCAGAACTGTTCAAAAATCACGTTGGGTTTGAATCCGACAGATACTTAGACTTTCATGAACTTGGGATGAAACTCTATTCAGAGGCTGTGGAAGAGACAGTTACAAGTGAAGAAGCACAAGGCCTATTTGACATGGCAGGAGGTAAGTTCCAAGAGATGACAGCTCTAGCATTGTTCAATTGGGGAAATGTGCATATGTCTAGGGCAAGGAAGAAAGTGTATTTAACTGAAGAAGATTCTTCTAGAGAGCATATGTATGAACAAATTAAAAGTTCGTATGAATGGGCGCAGAAAGAATATGCAAAAGCTGGAGAAAAATTTGAAGCAGCCATTAAAATTAAGTCAGATTTTTACGAAGGCTTTCTTGCTCTTGGTCAGCAACGATTCGAACAAGCGAAACTTTCTTGGTATTATGCACTCAGTAGTAATATAGATTTACCGACATGGCCCTCCACAGAGGTTCTTCATCTTTACAACAATGCTGAGGAGAATATGGAAAAAGGAATGCTGATATGGGAAGAATCGCAAGAGCAGCAGCTCAGTGAAGCCTCCACTACCGGGCTGGATGGCTTATTTAAAAATATGTCATCAGATGAAACTGCAGCGCTGGCGGCAAACATGAGGTCACAGATAAATCTCTTGTGGGGTACTATGCTGTATGAACGCTCACTTGTGGAATTCAAATTAGGGCTACCCGTATGGCATGAATCATTGGAAGCTGCGATTGAGAAGTTTGAACATGCAGGAGCTTCTCCAACAGATGTAGCTGTAATGTTGAAAAACCACTGTTCAAATAACACTGCTGTAGATGGCCTAGGTTTCAAAATTGATGAAATAGTACAGGCATGGAATGAGATGTATGAAGCTAAAAAGTTGCAGAAAGGAGTTTCATCCTTTCGTTTGGAACCCTTGTTCAGAAGAAGAGTATCGAAGATTTACCATGCCTTTGAGCTTGTATGA

Protein sequence

>Msa0287310

MGKKKKQVGETSEENGNHSNDGDKSPTAYDNDTLLFIATAQELKEEGNKMFQKRDLRGAMVRYEKALKLLPGNHIDVSYVRSNMAACYMQMGLGEYPRAINECNLALEVTPKYGKALLKRARCYEALNRLDLALRDVSAVLKMEPNNIMALEISDKVKSALEDRGLRVNDEKIELPPDYVEPSFDVPSEKAVKEKKHKKKSKKEKVKASDEIVEKQAEEKLEEKKEEDNIAPNKAEDDHLKEKKRNKSKKKAAKEKIDKQKEDVKEVNEEKSNGRSVDVPKKTAKLVFGDDIRWAQLPVNCSLFQLREIICDRFPSLGAVLVKYRDQEGDLITITSDEELKWAETGSQGSIRLYIVEANPNHDPFYEKLRLNDVEKVEMDNAPKNGCVVKSKEIVSSSCVEDWIIQFAELFKNHVGFESDRYLDFHELGMKLYSEAVEETVTSEEAQGLFDMAGGKFQEMTALALFNWGNVHMSRARKKVYLTEEDSSREHMYEQIKSSYEWAQKEYAKAGEKFEAAIKIKSDFYEGFLALGQQRFEQAKLSWYYALSSNIDLPTWPSTEVLHLYNNAEENMEKGMLIWEESQEQQLSEASTTGLDGLFKNMSSDETAALAANMRSQINLLWGTMLYERSLVEFKLGLPVWHESLEAAIEKFEHAGASPTDVAVMLKNHCSNNTAVDGLGFKIDEIVQAWNEMYEAKKLQKGVSSFRLEPLFRRRVSKIYHAFELV*