AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0563840

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0563840 MtrunA17_Chr8g0377671 97.213 287 8 0 1 287 1 287 0.0 548
Msa0563840 MtrunA17_Chr8g0377191 71.642 201 57 0 13 213 5 205 6.36e-97 283
Msa0563840 MtrunA17_Chr8g0377191 56.329 158 69 0 113 270 13 170 1.07e-52 171
Msa0563840 MtrunA17_Chr8g0377191 50.000 114 57 0 156 269 10 123 8.96e-29 109
Msa0563840 MtrunA17_Chr8g0385241 50.000 292 112 2 12 269 2 293 1.40e-82 254
Msa0563840 MtrunA17_Chr8g0385241 51.399 286 105 2 20 271 56 341 1.45e-82 254
Msa0563840 MtrunA17_Chr8g0385241 48.971 243 90 4 19 250 124 343 2.19e-62 202
Msa0563840 MtrunA17_Chr4g0059911 52.124 259 122 2 15 271 4 262 6.13e-81 249
Msa0563840 MtrunA17_Chr8g0382971 47.744 266 91 2 15 232 20 285 6.66e-69 217
Msa0563840 MtrunA17_Chr2g0316631 43.561 264 149 0 15 278 31 294 7.21e-69 216
Msa0563840 MtrunA17_Chr2g0316631 39.000 200 122 0 20 219 105 304 2.97e-44 152
Msa0563840 MtrunA17_Chr8g0377211 68.831 154 47 1 134 287 19 171 9.49e-68 208
Msa0563840 MtrunA17_Chr8g0377211 58.757 177 49 2 1 177 1 153 8.28e-61 190
Msa0563840 MtrunA17_Chr2g0316611 42.412 257 148 0 15 271 31 287 2.49e-67 214
Msa0563840 MtrunA17_Chr2g0316611 41.270 252 148 0 20 271 105 356 7.70e-60 194
Msa0563840 MtrunA17_Chr2g0316611 39.801 201 121 0 19 219 173 373 1.90e-42 149
Msa0563840 MtrunA17_Chr8g0380791 53.125 192 90 0 8 199 1 192 3.38e-63 212
Msa0563840 MtrunA17_Chr8g0380791 40.252 159 95 0 111 269 12 170 1.20e-31 124
Msa0563840 MtrunA17_Chr8g0380791 38.938 113 69 0 156 268 11 123 1.70e-15 76.6
Msa0563840 MtrunA17_Chr6g0467061 44.130 247 138 0 20 266 88 334 1.30e-62 201
Msa0563840 MtrunA17_Chr6g0467061 39.057 297 131 3 14 283 13 286 1.75e-58 190
Msa0563840 MtrunA17_Chr6g0467061 44.134 179 100 0 20 198 157 335 1.97e-39 140
Msa0563840 MtrunA17_Chr8g0383331 62.195 164 61 1 109 271 1 164 5.37e-62 193
Msa0563840 MtrunA17_Chr8g0383331 58.333 156 64 1 63 217 1 156 3.14e-56 178
Msa0563840 MtrunA17_Chr8g0383331 53.061 147 69 0 20 166 4 150 7.75e-46 152
Msa0563840 MtrunA17_Chr8g0383551 41.549 284 105 7 13 264 20 274 8.60e-54 179
Msa0563840 MtrunA17_Chr8g0383551 34.956 226 90 5 85 269 24 233 5.59e-27 108
Msa0563840 MtrunA17_Chr8g0377221 35.371 229 136 3 18 244 3 221 2.01e-40 141
Msa0563840 MtrunA17_Chr8g0377221 38.261 115 71 0 157 271 4 118 4.93e-19 84.7
Msa0563840 MtrunA17_Chr8g0377221 29.630 189 118 2 89 262 5 193 5.89e-18 81.6
Msa0563840 MtrunA17_Chr5g0424111 43.094 181 103 0 14 194 191 371 1.36e-39 143
Msa0563840 MtrunA17_Chr5g0424111 39.779 181 102 1 96 269 174 354 1.77e-34 129
Msa0563840 MtrunA17_Chr5g0424111 37.853 177 110 0 65 241 196 372 2.36e-30 118
Msa0563840 MtrunA17_Chr8g0353121 39.024 205 123 1 8 210 39 243 2.03e-39 146
Msa0563840 MtrunA17_Chr8g0353121 37.500 200 123 1 62 261 51 248 1.05e-30 121
Msa0563840 MtrunA17_Chr8g0353121 40.385 156 93 0 113 268 54 209 2.67e-28 114
Msa0563840 MtrunA17_Chr8g0353121 32.973 185 118 3 92 271 6 189 2.62e-18 85.1
Msa0563840 MtrunA17_Chr1g0180261 40.323 186 111 0 14 199 45 230 2.86e-37 140
Msa0563840 MtrunA17_Chr1g0180261 38.798 183 112 0 63 245 48 230 5.26e-30 119
Msa0563840 MtrunA17_Chr1g0180261 36.250 160 102 0 109 268 48 207 4.19e-25 105
Msa0563840 MtrunA17_Chr1g0180261 30.657 137 95 0 134 270 50 186 1.80e-13 70.5
Msa0563840 MtrunA17_Chr3g0082501 37.255 204 118 1 16 209 60 263 6.01e-37 139
Msa0563840 MtrunA17_Chr3g0082501 34.597 211 137 1 67 277 65 274 2.03e-31 123
Msa0563840 MtrunA17_Chr5g0424301 40.113 177 106 0 7 183 56 232 9.40e-37 138
Msa0563840 MtrunA17_Chr5g0424301 35.220 159 103 0 112 270 69 227 1.90e-25 105
Msa0563840 MtrunA17_Chr5g0424301 32.984 191 122 2 39 229 48 232 1.10e-24 103
Msa0563840 MtrunA17_Chr5g0424301 37.681 138 86 0 134 271 68 205 3.35e-22 96.3
Msa0563840 MtrunA17_Chr1g0171491 40.217 184 110 0 16 199 57 240 9.43e-36 135
Msa0563840 MtrunA17_Chr1g0171491 36.364 187 119 0 67 253 62 248 2.39e-30 120
Msa0563840 MtrunA17_Chr1g0171491 36.970 165 102 1 104 268 55 217 2.08e-26 108
Msa0563840 MtrunA17_Chr1g0171491 34.975 203 130 1 27 229 47 247 8.44e-26 107
Msa0563840 MtrunA17_Chr7g0233351 40.000 180 108 0 17 196 189 368 2.25e-34 129
Msa0563840 MtrunA17_Chr7g0233351 39.884 173 104 0 44 216 193 365 1.08e-31 122
Msa0563840 MtrunA17_Chr7g0233351 34.921 189 123 0 62 250 188 376 2.62e-31 121
Msa0563840 MtrunA17_Chr7g0233351 39.888 178 104 2 95 269 172 349 1.71e-30 119
Msa0563840 MtrunA17_Chr7g0233351 36.571 175 111 0 90 264 193 367 9.48e-28 111
Msa0563840 MtrunA17_Chr8g0336161 39.891 183 110 0 14 196 191 373 9.18e-34 127
Msa0563840 MtrunA17_Chr8g0336161 36.957 184 116 0 65 248 196 379 2.23e-30 118
Msa0563840 MtrunA17_Chr8g0336161 39.506 162 98 0 108 269 193 354 8.29e-28 111
Msa0563840 MtrunA17_Chr3g0114791 39.560 182 108 1 14 193 177 358 2.62e-33 126
Msa0563840 MtrunA17_Chr3g0114791 35.326 184 119 0 53 236 172 355 4.42e-30 117
Msa0563840 MtrunA17_Chr3g0114791 37.423 163 102 0 106 268 179 341 3.46e-29 115
Msa0563840 MtrunA17_Chr3g0114791 34.254 181 119 0 84 264 180 360 4.39e-25 103
Msa0563840 MtrunA17_Chr3g0114791 36.885 122 77 0 148 269 175 296 8.66e-16 77.0
Msa0563840 MtrunA17_Chr3g0115111 37.158 183 114 1 8 189 45 227 5.51e-32 124
Msa0563840 MtrunA17_Chr3g0115111 32.143 196 130 2 76 270 23 216 2.69e-26 108
Msa0563840 MtrunA17_Chr3g0115111 33.032 221 138 4 39 258 35 246 2.72e-26 108
Msa0563840 MtrunA17_Chr3g0115111 36.471 170 102 2 21 189 82 246 3.08e-22 96.7
Msa0563840 MtrunA17_Chr3g0115111 35.971 139 88 1 134 271 56 194 6.76e-20 89.7
Msa0563840 MtrunA17_Chr4g0055371 39.205 176 107 0 41 216 170 345 5.56e-32 122
Msa0563840 MtrunA17_Chr4g0055371 38.889 162 99 0 108 269 168 329 9.31e-28 110
Msa0563840 MtrunA17_Chr4g0055371 33.514 185 123 0 58 242 164 348 6.89e-27 108
Msa0563840 MtrunA17_Chr4g0055371 35.227 176 114 0 89 264 172 347 1.19e-25 105
Msa0563840 MtrunA17_Chr7g0256301 40.113 177 106 0 14 190 65 241 1.19e-31 124
Msa0563840 MtrunA17_Chr7g0256301 38.217 157 97 0 112 268 71 227 4.33e-24 102
Msa0563840 MtrunA17_Chr7g0256301 35.673 171 110 0 66 236 71 241 1.07e-23 100
Msa0563840 MtrunA17_Chr3g0131001 41.714 175 102 0 16 190 51 225 4.71e-31 122
Msa0563840 MtrunA17_Chr3g0131001 36.723 177 112 0 64 240 53 229 9.43e-21 92.0
Msa0563840 MtrunA17_Chr3g0131001 37.107 159 100 0 112 270 55 213 6.82e-19 86.7
Msa0563840 MtrunA17_Chr3g0131001 34.503 171 112 0 89 259 55 225 7.28e-19 86.7
Msa0563840 MtrunA17_Chr3g0131001 38.406 138 85 0 134 271 54 191 2.83e-18 84.7
Msa0563840 MtrunA17_Chr8g0377201 63.218 87 32 0 201 287 1 87 8.92e-31 110
Msa0563840 MtrunA17_Chr8g0377201 54.545 66 30 0 63 128 1 66 2.70e-17 75.1
Msa0563840 MtrunA17_Chr8g0377201 54.545 66 30 0 155 220 1 66 7.05e-17 73.9
Msa0563840 MtrunA17_Chr8g0377201 57.576 66 28 0 109 174 1 66 3.17e-16 72.4
Msa0563840 MtrunA17_Chr8g0377201 68.889 45 14 0 19 63 26 70 3.36e-13 64.3
Msa0563840 MtrunA17_Chr8g0385201 37.158 183 113 2 14 195 24 205 9.67e-29 115
Msa0563840 MtrunA17_Chr8g0385201 38.365 159 97 1 112 269 30 188 4.33e-27 110
Msa0563840 MtrunA17_Chr8g0385201 36.041 197 120 4 67 258 31 226 5.80e-25 104
Msa0563840 MtrunA17_Chr5g0398011 47.788 113 59 0 18 130 33 145 1.55e-27 104
Msa0563840 MtrunA17_Chr5g0398011 37.607 117 73 0 155 271 32 148 1.76e-20 85.5
Msa0563840 MtrunA17_Chr5g0398011 32.456 114 77 0 109 222 32 145 2.93e-13 66.2
Msa0563840 MtrunA17_Chr5g0398011 29.655 145 96 1 32 176 7 145 5.51e-13 65.5
Msa0563840 MtrunA17_Chr5g0398021 45.192 104 57 0 20 123 13 116 3.13e-24 95.9
Msa0563840 MtrunA17_Chr5g0398021 35.338 133 86 0 155 287 10 142 7.68e-20 84.0
Msa0563840 MtrunA17_Chr5g0398021 33.621 116 77 0 102 217 3 118 6.10e-16 73.6
Msa0563840 MtrunA17_Chr5g0398021 37.383 107 67 0 63 169 10 116 5.35e-15 70.9
Msa0563840 MtrunA17_Chr5g0398021 29.730 111 78 0 81 191 5 115 1.55e-14 69.7
Msa0563840 MtrunA17_Chr3g0085921 63.333 60 22 0 196 255 2 61 2.20e-19 81.6
Msa0563840 MtrunA17_Chr3g0085921 60.000 60 24 0 150 209 2 61 5.68e-18 77.8
Msa0563840 MtrunA17_Chr3g0085921 57.627 59 25 0 105 163 3 61 2.56e-17 75.9
Msa0563840 MtrunA17_Chr3g0085921 57.627 59 25 0 59 117 3 61 1.87e-15 70.9
Msa0563840 MtrunA17_Chr1g0210831 37.069 116 73 0 78 193 625 740 7.81e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0563840 AT1G60890.1 49.730 185 93 0 16 200 7 191 1.93e-54 188
Msa0563840 AT1G60890.1 41.209 182 107 0 65 246 10 191 7.34e-40 147
Msa0563840 AT1G60890.1 40.881 159 94 0 111 269 10 168 5.44e-33 128
Msa0563840 AT1G60890.2 49.730 185 93 0 16 200 19 203 2.05e-54 188
Msa0563840 AT1G60890.2 41.209 182 107 0 65 246 22 203 6.37e-40 148
Msa0563840 AT1G60890.2 40.881 159 94 0 111 269 22 180 4.50e-33 128
Msa0563840 AT1G10900.2 46.032 189 102 0 16 204 7 195 2.96e-49 174
Msa0563840 AT1G10900.2 38.798 183 112 0 64 246 9 191 1.04e-36 139
Msa0563840 AT1G10900.2 38.750 160 98 0 110 269 9 168 1.41e-29 118
Msa0563840 AT1G10900.1 46.032 189 102 0 16 204 7 195 2.96e-49 174
Msa0563840 AT1G10900.1 38.798 183 112 0 64 246 9 191 1.04e-36 139
Msa0563840 AT1G10900.1 38.750 160 98 0 110 269 9 168 1.41e-29 118
Msa0563840 AT1G10900.3 46.032 189 102 0 16 204 7 195 2.96e-49 174
Msa0563840 AT1G10900.3 38.798 183 112 0 64 246 9 191 1.04e-36 139
Msa0563840 AT1G10900.3 38.750 160 98 0 110 269 9 168 1.41e-29 118
Msa0563840 AT3G07960.2 40.541 185 110 0 16 200 23 207 5.35e-39 144
Msa0563840 AT3G07960.2 34.634 205 121 2 67 265 28 225 5.08e-32 125
Msa0563840 AT3G07960.2 40.764 157 93 0 112 268 27 183 2.05e-31 123
Msa0563840 AT3G07960.2 38.938 113 69 0 20 132 96 208 2.88e-14 73.2
Msa0563840 AT3G07960.4 40.541 185 110 0 16 200 23 207 5.35e-39 144
Msa0563840 AT3G07960.4 34.634 205 121 2 67 265 28 225 5.08e-32 125
Msa0563840 AT3G07960.4 40.764 157 93 0 112 268 27 183 2.05e-31 123
Msa0563840 AT3G07960.4 38.938 113 69 0 20 132 96 208 2.88e-14 73.2
Msa0563840 AT3G07960.3 40.541 185 110 0 16 200 23 207 7.66e-39 144
Msa0563840 AT3G07960.3 34.634 205 121 2 67 265 28 225 7.45e-32 124
Msa0563840 AT3G07960.3 40.764 157 93 0 112 268 27 183 2.91e-31 122
Msa0563840 AT3G07960.3 38.938 113 69 0 20 132 96 208 3.07e-14 73.2
Msa0563840 AT1G21980.1 41.714 175 102 0 16 190 72 246 8.68e-39 144
Msa0563840 AT1G21980.1 36.216 185 114 1 56 236 62 246 4.23e-30 119
Msa0563840 AT1G21980.1 37.500 160 100 0 110 269 74 233 2.77e-29 117
Msa0563840 AT1G21980.1 33.898 177 117 0 89 265 76 252 3.66e-25 105
Msa0563840 AT3G07960.1 40.541 185 110 0 16 200 23 207 1.44e-38 144
Msa0563840 AT3G07960.1 34.634 205 121 2 67 265 28 225 1.05e-31 124
Msa0563840 AT3G07960.1 40.764 157 93 0 112 268 27 183 4.01e-31 122
Msa0563840 AT3G07960.1 38.938 113 69 0 20 132 96 208 3.96e-14 72.8
Msa0563840 AT3G09920.3 41.935 186 108 0 14 199 47 232 2.65e-38 143
Msa0563840 AT3G09920.3 40.437 183 109 0 63 245 50 232 6.92e-33 128
Msa0563840 AT3G09920.3 39.752 161 97 0 109 269 50 210 2.06e-26 109
Msa0563840 AT3G09920.4 41.935 186 108 0 14 199 47 232 2.65e-38 143
Msa0563840 AT3G09920.4 40.437 183 109 0 63 245 50 232 6.92e-33 128
Msa0563840 AT3G09920.4 39.752 161 97 0 109 269 50 210 2.06e-26 109
Msa0563840 AT3G09920.2 41.935 186 108 0 14 199 47 232 2.65e-38 143
Msa0563840 AT3G09920.2 40.437 183 109 0 63 245 50 232 6.92e-33 128
Msa0563840 AT3G09920.2 39.752 161 97 0 109 269 50 210 2.06e-26 109
Msa0563840 AT3G09920.1 41.935 186 108 0 14 199 47 232 2.65e-38 143
Msa0563840 AT3G09920.1 40.437 183 109 0 63 245 50 232 6.92e-33 128
Msa0563840 AT3G09920.1 39.752 161 97 0 109 269 50 210 2.06e-26 109
Msa0563840 AT2G41210.1 36.765 204 120 1 16 210 66 269 1.43e-35 135
Msa0563840 AT2G41210.1 36.313 179 114 0 67 245 71 249 1.02e-28 115
Msa0563840 AT2G41210.1 36.364 165 103 1 104 268 64 226 3.63e-26 108
Msa0563840 AT3G56960.2 36.453 203 120 1 16 209 68 270 1.59e-35 135
Msa0563840 AT3G56960.2 35.754 179 115 0 67 245 73 251 1.15e-29 118
Msa0563840 AT3G56960.2 36.264 182 105 2 87 268 58 228 5.53e-28 113
Msa0563840 AT3G56960.1 36.453 203 120 1 16 209 68 270 1.59e-35 135
Msa0563840 AT3G56960.1 35.754 179 115 0 67 245 73 251 1.15e-29 118
Msa0563840 AT3G56960.1 36.264 182 105 2 87 268 58 228 5.53e-28 113
Msa0563840 AT1G77740.2 39.429 175 106 0 16 190 73 247 1.41e-34 132
Msa0563840 AT1G77740.2 37.647 170 106 0 67 236 78 247 5.30e-28 114
Msa0563840 AT1G77740.2 36.250 160 102 0 110 269 75 234 3.45e-27 111
Msa0563840 AT1G77740.2 33.333 171 114 0 89 259 77 247 2.89e-23 100
Msa0563840 AT1G77740.2 36.496 137 87 0 134 270 76 212 1.19e-21 95.1
Msa0563840 AT1G77740.1 39.429 175 106 0 16 190 73 247 1.41e-34 132
Msa0563840 AT1G77740.1 37.647 170 106 0 67 236 78 247 5.30e-28 114
Msa0563840 AT1G77740.1 36.250 160 102 0 110 269 75 234 3.45e-27 111
Msa0563840 AT1G77740.1 33.333 171 114 0 89 259 77 247 2.89e-23 100
Msa0563840 AT1G77740.1 36.496 137 87 0 134 270 76 212 1.19e-21 95.1
Msa0563840 AT1G21920.2 40.659 182 106 1 14 193 166 347 1.46e-34 129
Msa0563840 AT1G21920.2 36.413 184 117 0 53 236 161 344 7.59e-31 119
Msa0563840 AT1G21920.2 37.569 181 107 1 94 268 150 330 6.97e-29 113
Msa0563840 AT1G21920.1 40.659 182 106 1 14 193 166 347 2.32e-34 129
Msa0563840 AT1G21920.1 36.413 184 117 0 53 236 161 344 1.85e-30 118
Msa0563840 AT1G21920.1 37.569 181 107 1 94 268 150 330 1.79e-28 113
Msa0563840 AT1G77660.1 40.206 194 114 1 2 193 158 351 5.94e-34 128
Msa0563840 AT1G77660.1 37.222 180 113 0 57 236 169 348 6.44e-29 114
Msa0563840 AT1G77660.1 38.650 163 100 0 106 268 172 334 3.48e-26 107
Msa0563840 AT2G26420.1 41.463 164 96 0 14 177 47 210 9.00e-32 124
Msa0563840 AT2G26420.1 37.342 158 99 0 113 270 54 211 8.89e-26 107
Msa0563840 AT2G26420.1 36.957 184 114 1 67 248 54 237 2.72e-24 102
Msa0563840 AT2G26420.1 36.875 160 101 0 90 249 54 213 2.02e-22 97.4
Msa0563840 AT2G26420.2 41.463 164 96 0 14 177 47 210 1.02e-31 124
Msa0563840 AT2G26420.2 37.342 158 99 0 113 270 54 211 1.13e-25 107
Msa0563840 AT2G26420.2 36.957 184 114 1 67 248 54 237 3.38e-24 102
Msa0563840 AT2G26420.2 36.875 160 101 0 90 249 54 213 2.58e-22 97.1
Msa0563840 AT2G26420.3 41.463 164 96 0 14 177 47 210 1.02e-31 124
Msa0563840 AT2G26420.3 37.342 158 99 0 113 270 54 211 1.13e-25 107
Msa0563840 AT2G26420.3 36.957 184 114 1 67 248 54 237 3.38e-24 102
Msa0563840 AT2G26420.3 36.875 160 101 0 90 249 54 213 2.58e-22 97.1
Msa0563840 AT4G17080.1 40.000 175 105 0 19 193 256 430 1.47e-31 122
Msa0563840 AT4G17080.1 37.714 175 109 0 42 216 256 430 7.34e-29 115
Msa0563840 AT4G17080.1 38.012 171 106 0 99 269 244 414 1.82e-28 114
Msa0563840 AT4G17080.1 32.692 208 132 2 53 258 242 443 8.42e-28 112
Msa0563840 AT2G35170.1 39.766 171 103 0 23 193 229 399 7.97e-30 117
Msa0563840 AT2G35170.1 32.692 208 137 1 58 262 218 425 4.86e-27 110
Msa0563840 AT2G35170.1 38.824 170 104 0 47 216 230 399 7.80e-27 109
Msa0563840 AT2G35170.1 35.789 190 109 2 108 284 222 411 1.73e-26 108
Msa0563840 AT1G75010.1 34.921 126 82 0 68 193 604 729 5.26e-11 63.5

Find 109 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAACGTCTACATTGGAAAT+TGG 0.187316 4_1:-64302211 Msa0563840:CDS
GAAACGTCTACACTGGAAAT+TGG 0.199973 4_1:-64301935 Msa0563840:CDS
TTTAGAGAAGAGTGGTTAAA+AGG 0.215927 4_1:-64303070 Msa0563840:five_prime_UTR
TACACTGGAAATTGGGAAAA+AGG 0.217123 4_1:-64301927 Msa0563840:CDS
GGGATGTCTACGCTGGAAAT+TGG 0.240613 4_1:-64302073 Msa0563840:CDS
TACATTGGAAATTGGGAAAA+AGG 0.252400 4_1:-64302203 Msa0563840:CDS
ATTGATGATGATGATACTTT+AGG 0.276478 4_1:+64303103 None:intergenic
GAGATGTCTACACTGGAAAT+TGG 0.297839 4_1:-64301797 Msa0563840:CDS
TGAGATTCCTTCTAGCTATC+TGG 0.302934 4_1:-64300396 Msa0563840:three_prime_UTR
TGACCAACAACCATCGAAAA+TGG 0.303459 4_1:+64302277 None:intergenic
TACGCTGGAAATTGGGAAAA+AGG 0.309774 4_1:-64302065 Msa0563840:CDS
ACTGATGAACATGATGATTA+GGG 0.312807 4_1:-64300737 Msa0563840:three_prime_UTR
AATTAAGCTTCAGATGATAA+TGG 0.335808 4_1:+64301632 None:intergenic
TACTGATGAACATGATGATT+AGG 0.344048 4_1:-64300738 Msa0563840:three_prime_UTR
ATGGAAAAGGGATTATGAAT+TGG 0.347632 4_1:-64302034 Msa0563840:CDS
AATGGACTTTCGCATGGATA+TGG 0.353378 4_1:-64301978 Msa0563840:CDS
TACGAGGGCGATTGGGTTGA+TGG 0.368273 4_1:-64301720 Msa0563840:CDS
AATGGACATAAACATGGATA+TGG 0.369212 4_1:-64302116 Msa0563840:CDS
AGGGAAAGGGATTATGAATT+GGG 0.375715 4_1:-64302309 Msa0563840:CDS
TAGAAGACTTGTCAAAATAT+AGG 0.381462 4_1:+64300448 None:intergenic
GAGTTTATAGATTTGCAAAT+GGG 0.384860 4_1:-64302094 Msa0563840:CDS
CAGGGAAAGGGATTATGAAT+TGG 0.387625 4_1:-64302310 Msa0563840:CDS
TTCAAGGGTGAACTTCTTCA+TGG 0.393069 4_1:-64302398 Msa0563840:CDS
TTCGATGGTTGTTGGTCAAA+TGG 0.393393 4_1:-64302272 Msa0563840:CDS
AATGGACTTTCACATGGATA+TGG 0.393948 4_1:-64302254 Msa0563840:CDS
TGGAAAAGGGATTATGAATT+GGG 0.398832 4_1:-64302033 Msa0563840:CDS
ATTAAGCTTCAGATGATAAT+GGG 0.406796 4_1:+64301633 None:intergenic
TTCGATGGTTATTGGTCAAA+TGG 0.407614 4_1:-64301858 Msa0563840:CDS
TTCGATGGTTATTGGTCAAA+TGG 0.407614 4_1:-64302134 Msa0563840:CDS
TACGAGGGTGACTGGGTTGA+TGG 0.408545 4_1:-64302341 Msa0563840:CDS
AATAATAAAATGGATGGAAG+AGG 0.408648 4_1:-64301771 Msa0563840:CDS
GAACAATATACGAGGGTGAC+TGG 0.412789 4_1:-64302349 Msa0563840:CDS
ATGGAAAAGGGATTATGAAA+TGG 0.419433 4_1:-64302172 Msa0563840:CDS
TTCGATGGTTGCTGGTCAAA+TGG 0.420223 4_1:-64301996 Msa0563840:CDS
GGGATTATGAATTGGGCTAA+TGG 0.426829 4_1:-64302026 Msa0563840:CDS
AAGGGGAAAGGGATGGAAAA+GGG 0.430911 4_1:-64301908 Msa0563840:CDS
TGGGTTGATGGACAAATGAT+AGG 0.434531 4_1:-64301708 Msa0563840:CDS
GGAGTTTATAGATTTGCAAA+TGG 0.437498 4_1:-64302095 Msa0563840:CDS
AAAGGGGAAAGGGATGGAAA+AGG 0.442937 4_1:-64301909 Msa0563840:CDS
ATGGAAAAGGGATTATGAAA+TGG 0.443399 4_1:-64301896 Msa0563840:CDS
AAAGGAGAAAAGGATGGAAA+AGG 0.446263 4_1:-64302047 Msa0563840:CDS
AAACGTCTACACTGGAAATT+GGG 0.451480 4_1:-64301934 Msa0563840:CDS
AAACGTCTACATTGGAAATT+GGG 0.451574 4_1:-64302210 Msa0563840:CDS
AAGGAGAAAAGGATGGAAAA+GGG 0.451607 4_1:-64302046 Msa0563840:CDS
AGGGGAGGAGCAGTTGCTAT+AGG 0.454350 4_1:-64300718 Msa0563840:three_prime_UTR
GGGATTATGAATTGGTCAAA+TGG 0.455729 4_1:-64301750 Msa0563840:CDS
GAACAATGTACGAGGGCGAT+TGG 0.457462 4_1:-64301728 Msa0563840:CDS
AAGGTGAAAGGCATGGAAAA+GGG 0.462403 4_1:-64302184 Msa0563840:CDS
GGATGTCTACGCTGGAAATT+GGG 0.465315 4_1:-64302072 Msa0563840:CDS
CATCATCATCAATATATAGA+AGG 0.468056 4_1:-64303093 Msa0563840:five_prime_UTR
ATGGAAGAGGGATTATGAAT+TGG 0.469752 4_1:-64301758 Msa0563840:CDS
GGGATTATGAATTGGGGTAA+TGG 0.470324 4_1:-64302302 Msa0563840:CDS
AACAATGTACGAGGGCGATT+GGG 0.470740 4_1:-64301727 Msa0563840:CDS
TGTCTACATTGGTAAATTCA+AGG 0.474127 4_1:-64302414 Msa0563840:CDS
AAAGGTGAAAGGCATGGAAA+AGG 0.474168 4_1:-64302185 Msa0563840:CDS
CAAACAACCAGATAGCTAGA+AGG 0.478740 4_1:+64300389 None:intergenic
GACAGTGCATAATATATGTA+AGG 0.479384 4_1:+64303186 None:intergenic
GGAGTTTATAGATTTGCAAA+TGG 0.480472 4_1:-64301819 Msa0563840:CDS
CTATTTGTAGTTAAGTTCGC+GGG 0.481649 4_1:-64303164 Msa0563840:five_prime_UTR
GTGATTCATCATTGAGTGGT+TGG 0.482091 4_1:-64300343 Msa0563840:three_prime_UTR
TGGAAAAGGGATTATGAAAT+GGG 0.487859 4_1:-64301895 Msa0563840:CDS
TGGAAAAGGGATTATGAAAT+GGG 0.493424 4_1:-64302171 Msa0563840:CDS
GGAAAGTACACATGGACAAA+TGG 0.494048 4_1:-64302371 Msa0563840:CDS
GATGGAAAAGTGACAGGGAA+AGG 0.496612 4_1:-64302323 Msa0563840:CDS
GGAGTTTATAGATCTGCAAA+TGG 0.513797 4_1:-64301957 Msa0563840:CDS
GGAGTTTATAGATCTGCAAA+TGG 0.513797 4_1:-64302233 Msa0563840:CDS
TCAAATGGGGATGTCTACAT+TGG 0.518877 4_1:-64302425 Msa0563840:CDS
ATGCAAGTCATGGAGGATGA+TGG 0.528096 4_1:-64302601 Msa0563840:CDS
ATGGAAAAGTGACAGGGAAA+GGG 0.534561 4_1:-64302322 Msa0563840:CDS
GAGATCATGACTACAACAAA+GGG 0.536136 4_1:-64300793 Msa0563840:three_prime_UTR
TGGGTTGATGGAAAAGTGAC+AGG 0.540161 4_1:-64302329 Msa0563840:CDS
GCAAATGGAAACGTCTACAT+TGG 0.540510 4_1:-64302218 Msa0563840:CDS
AGCAGTTGCTATAGGAGTCT+AGG 0.545164 4_1:-64300710 Msa0563840:three_prime_UTR
ATAATAAAATGGATGGAAGA+GGG 0.546218 4_1:-64301770 Msa0563840:CDS
GATGGACAAATGATAGGGAA+TGG 0.550634 4_1:-64301702 Msa0563840:CDS
TCTATTTGTAGTTAAGTTCG+CGG 0.550789 4_1:-64303165 Msa0563840:five_prime_UTR
ATAGAAGGTTTAGAGAAGAG+TGG 0.560311 4_1:-64303078 Msa0563840:five_prime_UTR
AACAATATACGAGGGTGACT+GGG 0.562591 4_1:-64302348 Msa0563840:CDS
GGGTTGATGGACAAATGATA+GGG 0.565463 4_1:-64301707 Msa0563840:CDS
AGTTTATAGATTTGCAAATG+GGG 0.568661 4_1:-64302093 Msa0563840:CDS
GTCTACATTGGTAAATTCAA+GGG 0.570653 4_1:-64302413 Msa0563840:CDS
AATGGACAGAAACATGGCTA+TGG 0.574469 4_1:-64301840 Msa0563840:CDS
GGTGAACTTCTTCATGGCAT+CGG 0.576407 4_1:-64302392 Msa0563840:CDS
TGGTCAAATGGACTTTCACA+TGG 0.576790 4_1:-64302260 Msa0563840:CDS
GGGAATGGATTGATGATACC+AGG 0.577591 4_1:-64301687 Msa0563840:CDS
GAGTGAAAGAATGCAAGTCA+TGG 0.582412 4_1:-64302611 Msa0563840:CDS
GCAAATGGAAACGTCTACAC+TGG 0.588126 4_1:-64301942 Msa0563840:CDS
GCAAATGGGGATGTCTACGC+TGG 0.596613 4_1:-64302080 Msa0563840:CDS
TGGTCAAATGGACAGAAACA+TGG 0.596729 4_1:-64301846 Msa0563840:CDS
TGTTGTGATTCATCATTGAG+TGG 0.601446 4_1:-64300347 Msa0563840:three_prime_UTR
TGGTCAAATGGACTTTCGCA+TGG 0.604154 4_1:-64301984 Msa0563840:CDS
TGGTCAAATGGACATAAACA+TGG 0.607218 4_1:-64302122 Msa0563840:CDS
AGAGATCATGACTACAACAA+AGG 0.607283 4_1:-64300794 Msa0563840:three_prime_UTR
GCAAATGGAGATGTCTACAC+TGG 0.608648 4_1:-64301804 Msa0563840:CDS
GGGATTATGAAATGGGCTAA+CGG 0.622522 4_1:-64301888 Msa0563840:CDS
GGGATTATGAAATGGGCTAA+CGG 0.622522 4_1:-64302164 Msa0563840:CDS
GGGAAAGGGATTATGAATTG+GGG 0.649116 4_1:-64302308 Msa0563840:CDS
CTGATGAACATGATGATTAG+GGG 0.661023 4_1:-64300736 Msa0563840:three_prime_UTR
AAGTCATGGAGGATGATGGA+AGG 0.670085 4_1:-64302597 Msa0563840:intron
ATGAACATGATGATTAGGGG+AGG 0.677924 4_1:-64300733 Msa0563840:three_prime_UTR
GTCAAATGGAACAATGTACG+AGG 0.679412 4_1:-64301736 Msa0563840:CDS
ATGGCATCGGAAAGTACACA+TGG 0.681327 4_1:-64302379 Msa0563840:CDS
GTCTCAACTAATACTCAACA+AGG 0.683248 4_1:+64300487 None:intergenic
TATTTGTAGTTAAGTTCGCG+GGG 0.700724 4_1:-64303163 Msa0563840:five_prime_UTR
GACAAATGGAACAATATACG+AGG 0.707016 4_1:-64302357 Msa0563840:CDS
TCAAATGGAACAATGTACGA+GGG 0.718322 4_1:-64301735 Msa0563840:CDS
ACAAATGGAACAATATACGA+GGG 0.718645 4_1:-64302356 Msa0563840:CDS
GGGTTGATGGAAAAGTGACA+GGG 0.720292 4_1:-64302328 Msa0563840:CDS
TGAAAGAATGCAAGTCATGG+AGG 0.727592 4_1:-64302608 Msa0563840:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTGGAAAAATAATAAAA+TGG - chr4_1:64301739-64301758 Msa0563840:CDS 10.0%
!! GAAATCATAATCATAATAAA+TGG + chr4_1:64301035-64301054 None:intergenic 15.0%
!! TTTGATCTTTAATAGTATTA+TGG - chr4_1:64301935-64301954 Msa0563840:CDS 15.0%
!!! ACTTAATTATTTGATAATCA+TGG - chr4_1:64302019-64302038 Msa0563840:CDS 15.0%
!! ATTAAGCTTATAATGATAAT+TGG + chr4_1:64302360-64302379 None:intergenic 15.0%
!!! ATGATTATTAGTTTAAACTT+TGG - chr4_1:64302448-64302467 Msa0563840:intron 15.0%
!!! TATTATCTAGTTTTAGTTTT+TGG - chr4_1:64302904-64302923 Msa0563840:intron 15.0%
!!! ATTATTTTGTGTTTTCACAT+CGG - chr4_1:64300534-64300553 Msa0563840:three_prime_UTR 20.0%
!! TAATGTATTATCTTGATTCT+TGG - chr4_1:64300602-64300621 Msa0563840:three_prime_UTR 20.0%
!! AATGTATTATCTTGATTCTT+GGG - chr4_1:64300603-64300622 Msa0563840:three_prime_UTR 20.0%
!! AATTAAACAAACTCAAAACT+TGG + chr4_1:64300844-64300863 None:intergenic 20.0%
!! TGGAAAAATAATAAAATGGA+TGG - chr4_1:64301743-64301762 Msa0563840:CDS 20.0%
!! TTAATAGTATTATGGAAAAC+TGG - chr4_1:64301943-64301962 Msa0563840:CDS 20.0%
!! ATTATAAGCTTAATCCAAAT+AGG - chr4_1:64302366-64302385 Msa0563840:CDS 20.0%
!!! ATTATTAGTTTAAACTTTGG+TGG - chr4_1:64302451-64302470 Msa0563840:intron 20.0%
!!! ATTGATGATGATGATACTTT+AGG + chr4_1:64300420-64300439 None:intergenic 25.0%
! CATCATCATCAATATATAGA+AGG - chr4_1:64300427-64300446 Msa0563840:three_prime_UTR 25.0%
! AGTAGAAAAAAAGAGATCAT+CGG - chr4_1:64300474-64300493 Msa0563840:three_prime_UTR 25.0%
! TGAGAAATTATAGAGTAAAG+TGG + chr4_1:64300511-64300530 None:intergenic 25.0%
! AAAGCTTAATCCAAGAAAAA+AGG - chr4_1:64300562-64300581 Msa0563840:three_prime_UTR 25.0%
! AAGCTTAATCCAAGAAAAAA+GGG - chr4_1:64300563-64300582 Msa0563840:three_prime_UTR 25.0%
! ACAAAAAATTGAGATGAGAA+TGG + chr4_1:64300768-64300787 None:intergenic 25.0%
!! AAAGAGAAGTTCTTTTCAAA+TGG - chr4_1:64301080-64301099 Msa0563840:intron 25.0%
!! AAGAGAAGTTCTTTTCAAAT+GGG - chr4_1:64301081-64301100 Msa0563840:intron 25.0%
! GAGTTTATAGATTTGCAAAT+GGG - chr4_1:64301426-64301445 Msa0563840:intron 25.0%
! AGTTTATAGATTTGCAAATG+GGG - chr4_1:64301427-64301446 Msa0563840:intron 25.0%
! AATAATAAAATGGATGGAAG+AGG - chr4_1:64301749-64301768 Msa0563840:CDS 25.0%
! ATAATAAAATGGATGGAAGA+GGG - chr4_1:64301750-64301769 Msa0563840:CDS 25.0%
! ATTAAGCTTCAGATGATAAT+GGG + chr4_1:64301890-64301909 None:intergenic 25.0%
! AATTAAGCTTCAGATGATAA+TGG + chr4_1:64301891-64301910 None:intergenic 25.0%
! GAAAACTGGTATAATATTCA+TGG - chr4_1:64301957-64301976 Msa0563840:CDS 25.0%
!! GTCAAATAGTTTTCTATACA+GGG + chr4_1:64302045-64302064 None:intergenic 25.0%
!! AGTCAAATAGTTTTCTATAC+AGG + chr4_1:64302046-64302065 None:intergenic 25.0%
! TATTTGACTAATAGATCAGA+TGG - chr4_1:64302057-64302076 Msa0563840:CDS 25.0%
! ACTAATAAACTAAAACCTCA+TGG + chr4_1:64302126-64302145 None:intergenic 25.0%
! TTCTTATGTTAAGTAGACTT+GGG + chr4_1:64302176-64302195 None:intergenic 25.0%
! TTTCTTATGTTAAGTAGACT+TGG + chr4_1:64302177-64302196 None:intergenic 25.0%
! TATGTGTTTAGTGTTGTAAA+TGG - chr4_1:64302214-64302233 Msa0563840:CDS 25.0%
! TAGTGTTGTAAATGGAAAAT+TGG - chr4_1:64302222-64302241 Msa0563840:CDS 25.0%
!!! CTGACAGAAATTTTTTAGTA+TGG - chr4_1:64302250-64302269 Msa0563840:CDS 25.0%
! TATGGATAAAGAATGTAAAC+AGG - chr4_1:64302268-64302287 Msa0563840:CDS 25.0%
! TTATAGTAACTGTGTGTTTA+TGG - chr4_1:64302662-64302681 Msa0563840:intron 25.0%
! TAGAAGACTTGTCAAAATAT+AGG + chr4_1:64303075-64303094 None:intergenic 25.0%
TCTATTTGTAGTTAAGTTCG+CGG - chr4_1:64300355-64300374 Msa0563840:three_prime_UTR 30.0%
TTTAGAGAAGAGTGGTTAAA+AGG - chr4_1:64300450-64300469 Msa0563840:three_prime_UTR 30.0%
AAAAAGAGATCATCGGAAAA+AGG - chr4_1:64300481-64300500 Msa0563840:three_prime_UTR 30.0%
!!! TACACTAAACCCTTTTTTCT+TGG + chr4_1:64300575-64300594 None:intergenic 30.0%
GAACGTTAACAGAAATACTA+GGG + chr4_1:64300655-64300674 None:intergenic 30.0%
AGAACGTTAACAGAAATACT+AGG + chr4_1:64300656-64300675 None:intergenic 30.0%
CTAACAATGATTCACATTCT+GGG + chr4_1:64300686-64300705 None:intergenic 30.0%
ATCAACAAAACCCTGAATTA+GGG - chr4_1:64300720-64300739 Msa0563840:three_prime_UTR 30.0%
CTTAAATCAACCCTAATTCA+GGG + chr4_1:64300733-64300752 None:intergenic 30.0%
! AGAGAAGTTCTTTTCAAATG+GGG - chr4_1:64301082-64301101 Msa0563840:intron 30.0%
TGTCTACATTGGTAAATTCA+AGG - chr4_1:64301106-64301125 Msa0563840:intron 30.0%
GTCTACATTGGTAAATTCAA+GGG - chr4_1:64301107-64301126 Msa0563840:intron 30.0%
ACAAATGGAACAATATACGA+GGG - chr4_1:64301164-64301183 Msa0563840:intron 30.0%
AAACGTCTACATTGGAAATT+GGG - chr4_1:64301310-64301329 Msa0563840:intron 30.0%
TACATTGGAAATTGGGAAAA+AGG - chr4_1:64301317-64301336 Msa0563840:intron 30.0%
ATGGAAAAGGGATTATGAAA+TGG - chr4_1:64301348-64301367 Msa0563840:intron 30.0%
TGGAAAAGGGATTATGAAAT+GGG - chr4_1:64301349-64301368 Msa0563840:intron 30.0%
AATGGACATAAACATGGATA+TGG - chr4_1:64301404-64301423 Msa0563840:intron 30.0%
GGAGTTTATAGATTTGCAAA+TGG - chr4_1:64301425-64301444 Msa0563840:intron 30.0%
AAATTGGGAAAAAGGAGAAA+AGG - chr4_1:64301463-64301482 Msa0563840:intron 30.0%
ATGGAAAAGGGATTATGAAT+TGG - chr4_1:64301486-64301505 Msa0563840:intron 30.0%
TGGAAAAGGGATTATGAATT+GGG - chr4_1:64301487-64301506 Msa0563840:intron 30.0%
ATGGAAAAGGGATTATGAAA+TGG - chr4_1:64301348-64301367 Msa0563840:intron 30.0%
TGGAAAAGGGATTATGAAAT+GGG - chr4_1:64301349-64301368 Msa0563840:intron 30.0%
GGAGTTTATAGATTTGCAAA+TGG - chr4_1:64301425-64301444 Msa0563840:intron 30.0%
AAAAATGTACATAGAAAGCC+TGG + chr4_1:64301854-64301873 None:intergenic 30.0%
GCTTCAAGTCTTTATGAAAA+TGG - chr4_1:64302079-64302098 Msa0563840:CDS 30.0%
CACAGAAAATATAGATGACA+GGG - chr4_1:64302308-64302327 Msa0563840:CDS 30.0%
AAAACTGAAACCCTTTAGAT+AGG + chr4_1:64302336-64302355 None:intergenic 30.0%
ATAAGCTTAATCCAAATAGG+AGG - chr4_1:64302369-64302388 Msa0563840:CDS 30.0%
TGATAAGTATCTAAGCACTT+GGG + chr4_1:64302533-64302552 None:intergenic 30.0%
TACTGATGAACATGATGATT+AGG - chr4_1:64302782-64302801 Msa0563840:intron 30.0%
ACTGATGAACATGATGATTA+GGG - chr4_1:64302783-64302802 Msa0563840:intron 30.0%
!!! GGTAAGATGTTTTGAGAATT+AGG + chr4_1:64303015-64303034 None:intergenic 30.0%
CTATTTGTAGTTAAGTTCGC+GGG - chr4_1:64300356-64300375 Msa0563840:three_prime_UTR 35.0%
TATTTGTAGTTAAGTTCGCG+GGG - chr4_1:64300357-64300376 Msa0563840:three_prime_UTR 35.0%
ATAGAAGGTTTAGAGAAGAG+TGG - chr4_1:64300442-64300461 Msa0563840:three_prime_UTR 35.0%
! ATTTTGTGTTTTCACATCGG+AGG - chr4_1:64300537-64300556 Msa0563840:three_prime_UTR 35.0%
GCTAACAATGATTCACATTC+TGG + chr4_1:64300687-64300706 None:intergenic 35.0%
CATCAACAAAACCCTGAATT+AGG - chr4_1:64300719-64300738 Msa0563840:three_prime_UTR 35.0%
CCTTAAATCAACCCTAATTC+AGG + chr4_1:64300734-64300753 None:intergenic 35.0%
! CCTGAATTAGGGTTGATTTA+AGG - chr4_1:64300731-64300750 Msa0563840:three_prime_UTR 35.0%
ACAAACTCAAAACTTGGAAG+AGG + chr4_1:64300838-64300857 None:intergenic 35.0%
TCAAATTCAGTTCAGAAAGC+TGG + chr4_1:64300872-64300891 None:intergenic 35.0%
TGAATTAGATAGAGTAGCTG+TGG + chr4_1:64300964-64300983 None:intergenic 35.0%
GACAAATGGAACAATATACG+AGG - chr4_1:64301163-64301182 Msa0563840:intron 35.0%
AGGGAAAGGGATTATGAATT+GGG - chr4_1:64301211-64301230 Msa0563840:intron 35.0%
! AATGGACTTTCACATGGATA+TGG - chr4_1:64301266-64301285 Msa0563840:intron 35.0%
GGAGTTTATAGATCTGCAAA+TGG - chr4_1:64301287-64301306 Msa0563840:intron 35.0%
GAAACGTCTACATTGGAAAT+TGG - chr4_1:64301309-64301328 Msa0563840:intron 35.0%
GAAATTGGGAAAAAGGTGAA+AGG - chr4_1:64301324-64301343 Msa0563840:intron 35.0%
! GTGATGTTTTCGATGGTTAT+TGG - chr4_1:64301378-64301397 Msa0563840:intron 35.0%
TTCGATGGTTATTGGTCAAA+TGG - chr4_1:64301386-64301405 Msa0563840:intron 35.0%
TGGTCAAATGGACATAAACA+TGG - chr4_1:64301398-64301417 Msa0563840:intron 35.0%
AAAGGAGAAAAGGATGGAAA+AGG - chr4_1:64301473-64301492 Msa0563840:intron 35.0%
AAGGAGAAAAGGATGGAAAA+GGG - chr4_1:64301474-64301493 Msa0563840:intron 35.0%
GGAGTTTATAGATCTGCAAA+TGG - chr4_1:64301287-64301306 Msa0563840:intron 35.0%
AAACGTCTACACTGGAAATT+GGG - chr4_1:64301586-64301605 Msa0563840:intron 35.0%
TACACTGGAAATTGGGAAAA+AGG - chr4_1:64301593-64301612 Msa0563840:intron 35.0%
ACACTGGAAATTGGGAAAAA+GGG - chr4_1:64301594-64301613 Msa0563840:intron 35.0%
AAATTGGGAAAAAGGGGAAA+GGG - chr4_1:64301601-64301620 Msa0563840:intron 35.0%
! GTGATGTTTTCGATGGTTAT+TGG - chr4_1:64301378-64301397 Msa0563840:intron 35.0%
TTCGATGGTTATTGGTCAAA+TGG - chr4_1:64301386-64301405 Msa0563840:intron 35.0%
ATGGAAGAGGGATTATGAAT+TGG - chr4_1:64301762-64301781 Msa0563840:CDS 35.0%
GGGATTATGAATTGGTCAAA+TGG - chr4_1:64301770-64301789 Msa0563840:CDS 35.0%
TCAAATGGAACAATGTACGA+GGG - chr4_1:64301785-64301804 Msa0563840:CDS 35.0%
! CCTGTCATCTATATTTTCTG+TGG + chr4_1:64302310-64302329 None:intergenic 35.0%
CCACAGAAAATATAGATGAC+AGG - chr4_1:64302307-64302326 Msa0563840:CDS 35.0%
AAACTAAACAGCCTCCTATT+TGG + chr4_1:64302383-64302402 None:intergenic 35.0%
!!! ACTTTGGTGGACTATTTTAG+TGG - chr4_1:64302464-64302483 Msa0563840:intron 35.0%
GCTATCAAAGGTGAATAATG+AGG + chr4_1:64302490-64302509 None:intergenic 35.0%
!! CTTTGATAGCTAGCTTTCAA+GGG - chr4_1:64302500-64302519 Msa0563840:intron 35.0%
GATAAGTATCTAAGCACTTG+GGG + chr4_1:64302532-64302551 None:intergenic 35.0%
CTGATAAGTATCTAAGCACT+TGG + chr4_1:64302534-64302553 None:intergenic 35.0%
AGATACTTATCAGAACGATG+TGG - chr4_1:64302541-64302560 Msa0563840:intron 35.0%
AGAGATCATGACTACAACAA+AGG - chr4_1:64302726-64302745 Msa0563840:intron 35.0%
GAGATCATGACTACAACAAA+GGG - chr4_1:64302727-64302746 Msa0563840:intron 35.0%
CTGATGAACATGATGATTAG+GGG - chr4_1:64302784-64302803 Msa0563840:intron 35.0%
GTCTCAACTAATACTCAACA+AGG + chr4_1:64303036-64303055 None:intergenic 35.0%
TGTTGTGATTCATCATTGAG+TGG - chr4_1:64303173-64303192 Msa0563840:five_prime_UTR 35.0%
GAGTGAAAGAATGCAAGTCA+TGG - chr4_1:64300909-64300928 Msa0563840:intron 40.0%
TGAAAGAATGCAAGTCATGG+AGG - chr4_1:64300912-64300931 Msa0563840:intron 40.0%
TCAAATGGGGATGTCTACAT+TGG - chr4_1:64301095-64301114 Msa0563840:intron 40.0%
TTCAAGGGTGAACTTCTTCA+TGG - chr4_1:64301122-64301141 Msa0563840:intron 40.0%
! GGAAAGTACACATGGACAAA+TGG - chr4_1:64301149-64301168 Msa0563840:intron 40.0%
AACAATATACGAGGGTGACT+GGG - chr4_1:64301172-64301191 Msa0563840:intron 40.0%
ATGGAAAAGTGACAGGGAAA+GGG - chr4_1:64301198-64301217 Msa0563840:intron 40.0%
CAGGGAAAGGGATTATGAAT+TGG - chr4_1:64301210-64301229 Msa0563840:intron 40.0%
GGGAAAGGGATTATGAATTG+GGG - chr4_1:64301212-64301231 Msa0563840:intron 40.0%
GGGATTATGAATTGGGGTAA+TGG - chr4_1:64301218-64301237 Msa0563840:intron 40.0%
TGACCAACAACCATCGAAAA+TGG + chr4_1:64301246-64301265 None:intergenic 40.0%
TTCGATGGTTGTTGGTCAAA+TGG - chr4_1:64301248-64301267 Msa0563840:intron 40.0%
! TGGTCAAATGGACTTTCACA+TGG - chr4_1:64301260-64301279 Msa0563840:intron 40.0%
GCAAATGGAAACGTCTACAT+TGG - chr4_1:64301302-64301321 Msa0563840:intron 40.0%
! AAAGGTGAAAGGCATGGAAA+AGG - chr4_1:64301335-64301354 Msa0563840:intron 40.0%
! AAGGTGAAAGGCATGGAAAA+GGG - chr4_1:64301336-64301355 Msa0563840:intron 40.0%
!! GGGATTATGAAATGGGCTAA+CGG - chr4_1:64301356-64301375 Msa0563840:intron 40.0%
TACGCTGGAAATTGGGAAAA+AGG - chr4_1:64301455-64301474 Msa0563840:intron 40.0%
TGGGAAAAAGGAGAAAAGGA+TGG - chr4_1:64301467-64301486 Msa0563840:intron 40.0%
GGGATTATGAATTGGGCTAA+TGG - chr4_1:64301494-64301513 Msa0563840:intron 40.0%
!!! GCTAATGGTGCTATTTTCGA+TGG - chr4_1:64301509-64301528 Msa0563840:intron 40.0%
! AATGGACTTTCGCATGGATA+TGG - chr4_1:64301542-64301561 Msa0563840:intron 40.0%
GAAACGTCTACACTGGAAAT+TGG - chr4_1:64301585-64301604 Msa0563840:intron 40.0%
CACTGGAAATTGGGAAAAAG+GGG - chr4_1:64301595-64301614 Msa0563840:intron 40.0%
GAAATTGGGAAAAAGGGGAA+AGG - chr4_1:64301600-64301619 Msa0563840:intron 40.0%
!! GGGATTATGAAATGGGCTAA+CGG - chr4_1:64301356-64301375 Msa0563840:intron 40.0%
TGGTCAAATGGACAGAAACA+TGG - chr4_1:64301674-64301693 Msa0563840:CDS 40.0%
AATGGACAGAAACATGGCTA+TGG - chr4_1:64301680-64301699 Msa0563840:CDS 40.0%
GAGATGTCTACACTGGAAAT+TGG - chr4_1:64301723-64301742 Msa0563840:CDS 40.0%
GTCAAATGGAACAATGTACG+AGG - chr4_1:64301784-64301803 Msa0563840:CDS 40.0%
!! TGGGTTGATGGACAAATGAT+AGG - chr4_1:64301812-64301831 Msa0563840:CDS 40.0%
!! GGGTTGATGGACAAATGATA+GGG - chr4_1:64301813-64301832 Msa0563840:CDS 40.0%
GATGGACAAATGATAGGGAA+TGG - chr4_1:64301818-64301837 Msa0563840:CDS 40.0%
ACTGTTCGATAAGTTCCATG+AGG - chr4_1:64302108-64302127 Msa0563840:CDS 40.0%
ATGACAGGGTTCCTATCTAA+AGG - chr4_1:64302322-64302341 Msa0563840:CDS 40.0%
TGACAGGGTTCCTATCTAAA+GGG - chr4_1:64302323-64302342 Msa0563840:CDS 40.0%
CCTTGAAAGCTAGCTATCAA+AGG + chr4_1:64302502-64302521 None:intergenic 40.0%
!! CCTTTGATAGCTAGCTTTCA+AGG - chr4_1:64302499-64302518 Msa0563840:intron 40.0%
GAACGATGTGGTGTGATATA+CGG - chr4_1:64302553-64302572 Msa0563840:intron 40.0%
ATGAACATGATGATTAGGGG+AGG - chr4_1:64302787-64302806 Msa0563840:intron 40.0%
! TGAGATTCCTTCTAGCTATC+TGG - chr4_1:64303124-64303143 Msa0563840:five_prime_UTR 40.0%
CAAACAACCAGATAGCTAGA+AGG + chr4_1:64303134-64303153 None:intergenic 40.0%
GTGATTCATCATTGAGTGGT+TGG - chr4_1:64303177-64303196 Msa0563840:five_prime_UTR 40.0%
ATGCAAGTCATGGAGGATGA+TGG - chr4_1:64300919-64300938 Msa0563840:intron 45.0%
!! AAGTCATGGAGGATGATGGA+AGG - chr4_1:64300923-64300942 Msa0563840:intron 45.0%
GGTGAACTTCTTCATGGCAT+CGG - chr4_1:64301128-64301147 Msa0563840:intron 45.0%
! ATGGCATCGGAAAGTACACA+TGG - chr4_1:64301141-64301160 Msa0563840:intron 45.0%
GAACAATATACGAGGGTGAC+TGG - chr4_1:64301171-64301190 Msa0563840:intron 45.0%
!! TGGGTTGATGGAAAAGTGAC+AGG - chr4_1:64301191-64301210 Msa0563840:intron 45.0%
!! GGGTTGATGGAAAAGTGACA+GGG - chr4_1:64301192-64301211 Msa0563840:intron 45.0%
GATGGAAAAGTGACAGGGAA+AGG - chr4_1:64301197-64301216 Msa0563840:intron 45.0%
!!! GGTAATGGTGCCATTTTCGA+TGG - chr4_1:64301233-64301252 Msa0563840:intron 45.0%
!!! GTGCCATTTTCGATGGTTGT+TGG - chr4_1:64301240-64301259 Msa0563840:intron 45.0%
TGGGAAAAAGGTGAAAGGCA+TGG - chr4_1:64301329-64301348 Msa0563840:intron 45.0%
! GCTAACGGTGATGTTTTCGA+TGG - chr4_1:64301371-64301390 Msa0563840:intron 45.0%
GGATGTCTACGCTGGAAATT+GGG - chr4_1:64301448-64301467 Msa0563840:intron 45.0%
!!! GTGCTATTTTCGATGGTTGC+TGG - chr4_1:64301516-64301535 Msa0563840:intron 45.0%
TTCGATGGTTGCTGGTCAAA+TGG - chr4_1:64301524-64301543 Msa0563840:intron 45.0%
! TGGTCAAATGGACTTTCGCA+TGG - chr4_1:64301536-64301555 Msa0563840:intron 45.0%
GCAAATGGAAACGTCTACAC+TGG - chr4_1:64301578-64301597 Msa0563840:intron 45.0%
AAAGGGGAAAGGGATGGAAA+AGG - chr4_1:64301611-64301630 Msa0563840:intron 45.0%
AAGGGGAAAGGGATGGAAAA+GGG - chr4_1:64301612-64301631 Msa0563840:intron 45.0%
! GCTAACGGTGATGTTTTCGA+TGG - chr4_1:64301371-64301390 Msa0563840:intron 45.0%
GCAAATGGAGATGTCTACAC+TGG - chr4_1:64301716-64301735 Msa0563840:CDS 45.0%
AACAATGTACGAGGGCGATT+GGG - chr4_1:64301793-64301812 Msa0563840:CDS 45.0%
!! GGGAATGGATTGATGATACC+AGG - chr4_1:64301833-64301852 Msa0563840:CDS 45.0%
!! TGATAGCTAGCTTTCAAGGG+AGG - chr4_1:64302503-64302522 Msa0563840:intron 45.0%
AGCAGTTGCTATAGGAGTCT+AGG - chr4_1:64302810-64302829 Msa0563840:intron 45.0%
GGGATGTCTACGCTGGAAAT+TGG - chr4_1:64301447-64301466 Msa0563840:intron 50.0%
TGGGAAAAAGGGGAAAGGGA+TGG - chr4_1:64301605-64301624 Msa0563840:intron 50.0%
GAACAATGTACGAGGGCGAT+TGG - chr4_1:64301792-64301811 Msa0563840:CDS 50.0%
! TACGAGGGTGACTGGGTTGA+TGG - chr4_1:64301179-64301198 Msa0563840:intron 55.0%
GCAAATGGGGATGTCTACGC+TGG - chr4_1:64301440-64301459 Msa0563840:intron 55.0%
!! TACGAGGGCGATTGGGTTGA+TGG - chr4_1:64301800-64301819 Msa0563840:CDS 55.0%
AGGGGAGGAGCAGTTGCTAT+AGG - chr4_1:64302802-64302821 Msa0563840:intron 55.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 64300335 64303207 64300335 ID=Msa0563840;Name=Msa0563840
chr4_1 mRNA 64300335 64303207 64300335 ID=Msa0563840-mRNA-1;Parent=Msa0563840;Name=Msa0563840-mRNA-1;_AED=0.01;_eAED=0.01;_QI=202|1|1|1|0.33|0|4|505|287
chr4_1 exon 64300335 64300814 64300335 ID=Msa0563840-mRNA-1:exon:20531;Parent=Msa0563840-mRNA-1
chr4_1 exon 64301616 64302466 64301616 ID=Msa0563840-mRNA-1:exon:20530;Parent=Msa0563840-mRNA-1
chr4_1 exon 64302598 64302669 64302598 ID=Msa0563840-mRNA-1:exon:20529;Parent=Msa0563840-mRNA-1
chr4_1 exon 64303040 64303207 64303040 ID=Msa0563840-mRNA-1:exon:20528;Parent=Msa0563840-mRNA-1
chr4_1 five_prime_UTR 64303040 64303207 64303040 ID=Msa0563840-mRNA-1:five_prime_utr;Parent=Msa0563840-mRNA-1
chr4_1 five_prime_UTR 64302636 64302669 64302636 ID=Msa0563840-mRNA-1:five_prime_utr;Parent=Msa0563840-mRNA-1
chr4_1 CDS 64302598 64302635 64302598 ID=Msa0563840-mRNA-1:cds;Parent=Msa0563840-mRNA-1
chr4_1 CDS 64301641 64302466 64301641 ID=Msa0563840-mRNA-1:cds;Parent=Msa0563840-mRNA-1
chr4_1 three_prime_UTR 64301616 64301640 64301616 ID=Msa0563840-mRNA-1:three_prime_utr;Parent=Msa0563840-mRNA-1
chr4_1 three_prime_UTR 64300335 64300814 64300335 ID=Msa0563840-mRNA-1:three_prime_utr;Parent=Msa0563840-mRNA-1
Gene Sequence

>Msa0563840

ATGAGTGAAAGAATGCAAGTCATGGAGGATGATGGAAGTGTTAAAGAGAAGTTCTTTTCAAATGGGGATGTCTACATTGGTAAATTCAAGGGTGAACTTCTTCATGGCATCGGAAAGTACACATGGACAAATGGAACAATATACGAGGGTGACTGGGTTGATGGAAAAGTGACAGGGAAAGGGATTATGAATTGGGGTAATGGTGCCATTTTCGATGGTTGTTGGTCAAATGGACTTTCACATGGATATGGAGTTTATAGATCTGCAAATGGAAACGTCTACATTGGAAATTGGGAAAAAGGTGAAAGGCATGGAAAAGGGATTATGAAATGGGCTAACGGTGATGTTTTCGATGGTTATTGGTCAAATGGACATAAACATGGATATGGAGTTTATAGATTTGCAAATGGGGATGTCTACGCTGGAAATTGGGAAAAAGGAGAAAAGGATGGAAAAGGGATTATGAATTGGGCTAATGGTGCTATTTTCGATGGTTGCTGGTCAAATGGACTTTCGCATGGATATGGAGTTTATAGATCTGCAAATGGAAACGTCTACACTGGAAATTGGGAAAAAGGGGAAAGGGATGGAAAAGGGATTATGAAATGGGCTAACGGTGATGTTTTCGATGGTTATTGGTCAAATGGACAGAAACATGGCTATGGAGTTTATAGATTTGCAAATGGAGATGTCTACACTGGAAATTGGAAAAATAATAAAATGGATGGAAGAGGGATTATGAATTGGTCAAATGGAACAATGTACGAGGGCGATTGGGTTGATGGACAAATGATAGGGAATGGATTGATGATACCAGGCTTTCTATGTACATTTTTCAAGACTATCAGACCCATTATCATCTGA
Protein sequence

>Msa0563840

MSERMQVMEDDGSVKEKFFSNGDVYIGKFKGELLHGIGKYTWTNGTIYEGDWVDGKVTGKGIMNWGNGAIFDGCWSNGLSHGYGVYRSANGNVYIGNWEKGERHGKGIMKWANGDVFDGYWSNGHKHGYGVYRFANGDVYAGNWEKGEKDGKGIMNWANGAIFDGCWSNGLSHGYGVYRSANGNVYTGNWEKGERDGKGIMKWANGDVFDGYWSNGQKHGYGVYRFANGDVYTGNWKNNKMDGRGIMNWSNGTMYEGDWVDGQMIGNGLMIPGFLCTFFKTIRPIII*