AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0315120

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0315120 MtrunA17_Chr2g0279401 93.659 205 2 1 1 194 337 541 6.69e-137 393
Msa0315120 MtrunA17_Chr4g0050151 80.000 205 29 2 1 193 330 534 2.71e-116 340
Msa0315120 MtrunA17_Chr2g0327771 72.973 185 39 1 2 175 323 507 2.44e-95 286
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0315120 AT5G64300.1 80.645 186 25 1 1 175 344 529 1.13e-109 324
Msa0315120 AT5G59750.1 70.053 187 45 1 2 177 319 505 7.95e-92 277
Msa0315120 AT5G59750.2 70.053 187 45 1 2 177 353 539 2.17e-91 277
Msa0315120 AT2G22450.1 61.935 155 52 3 1 150 322 474 2.76e-61 197
Msa0315120 AT5G59750.3 80.357 112 22 0 2 113 319 430 2.23e-60 194

Find 75 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCTATGGTTTGACTGTTTC+TGG 0.114584 2_4:+11302540 Msa0315120:CDS
GATACTTCTTGTTGCTTATT+TGG 0.133035 2_4:+11301487 Msa0315120:five_prime_UTR
CAGGTTTGCTTGTCTTTCTT+TGG 0.178056 2_4:+11302948 Msa0315120:three_prime_UTR
CATTGTATTACCTTGCTTAT+TGG 0.184117 2_4:+11302988 Msa0315120:three_prime_UTR
AGAGACCAAACGTGTGAAAA+TGG 0.231254 2_4:+11302611 Msa0315120:CDS
ACAAGGAGATTAAAACTTAT+TGG 0.231921 2_4:+11301515 Msa0315120:five_prime_UTR
TGTCTAACTGGAGACATATT+TGG 0.294019 2_4:+11301830 Msa0315120:CDS
GTGAAAATGGGCCACGTCTA+TGG 0.322087 2_4:+11302624 Msa0315120:CDS
GCTCCGTGCTTATAACTTAC+AGG 0.341829 2_4:+11301976 Msa0315120:CDS
TTCTTTGGTGTCTGTTACAG+AGG 0.383488 2_4:+11302963 Msa0315120:three_prime_UTR
GTATGATATATGTAGGTAAT+AGG 0.383546 2_4:-11302733 None:intergenic
GAAGAATCAAATGTTGTTGC+TGG 0.405283 2_4:+11302687 Msa0315120:CDS
CAGTCAAACCATAGCCTTTG+AGG 0.405833 2_4:-11302533 None:intergenic
ATTTGATTCTTCAACAGTAT+TGG 0.413785 2_4:-11302676 None:intergenic
TTTCAGATACTGAGGGATCT+AGG 0.416479 2_4:+11302462 Msa0315120:intron
CTCTAAGTATCTCTTGTTCT+CGG 0.416752 2_4:-11302592 None:intergenic
GTACACTCAGAGTGTCTAAC+TGG 0.418207 2_4:+11301818 Msa0315120:CDS
TGAGCATATTGCAATGGTTA+AGG 0.423972 2_4:+11301654 Msa0315120:CDS
GTGCCTTCTTGAAGAAAATC+AGG 0.434371 2_4:-11302853 None:intergenic
ACAAAATATGTTGGCCTCAA+AGG 0.435305 2_4:+11302519 Msa0315120:CDS
CTGATTTCCACAGTCACATC+TGG 0.442660 2_4:-11301858 None:intergenic
AGCAAACCTGAAAATTGTAT+TGG 0.457418 2_4:-11302935 None:intergenic
CCTGAAAATTGTATTGGTAA+TGG 0.458617 2_4:-11302929 None:intergenic
AAGAAAGAGAGATCAATTAG+TGG 0.461335 2_4:+11301552 Msa0315120:five_prime_UTR
CAACTCCTCATTGGCTTCTA+CGG 0.466646 2_4:-11302014 None:intergenic
AGGCAATCCCAACTCCTCAT+TGG 0.470707 2_4:-11302023 None:intergenic
ATGGCCTTATCTATAATGAT+TGG 0.471805 2_4:-11302910 None:intergenic
GAAGGAAGAGGTATTGGATT+GGG 0.477603 2_4:+11301947 Msa0315120:CDS
CGTAGAAGCCAATGAGGAGT+TGG 0.481823 2_4:+11302015 Msa0315120:CDS
GAGACCAAACGTGTGAAAAT+GGG 0.484788 2_4:+11302612 Msa0315120:CDS
ATGGTTTGACTGTTTCTGGT+AGG 0.495319 2_4:+11302544 Msa0315120:CDS
CAGATTGAGGCTGCCGGTAG+GGG 0.496302 2_4:+11301899 Msa0315120:CDS
TATGTTGGCCTCAAAGGCTA+TGG 0.502238 2_4:+11302525 Msa0315120:CDS
GGACATGAAGGAAGAGGTAT+TGG 0.502608 2_4:+11301941 Msa0315120:CDS
AGTGCCAATCATTATAGATA+AGG 0.507591 2_4:+11302906 Msa0315120:three_prime_UTR
ACAGATTGAGGCTGCCGGTA+GGG 0.509746 2_4:+11301898 Msa0315120:CDS
GTTATAAGTCTCTGTTAGAC+GGG 0.512110 2_4:+11301629 Msa0315120:CDS
ACGTGATAAGAGTTAACAAC+GGG 0.514164 2_4:-11302569 None:intergenic
GCTTATAACTTACAGGATGA+TGG 0.518705 2_4:+11301983 Msa0315120:CDS
TATATCATACACAACAAAAG+AGG 0.522768 2_4:+11302746 Msa0315120:three_prime_UTR
GTAATTTGACAGGGTGACAT+TGG 0.522822 2_4:+11301770 Msa0315120:intron
AACAATCCAACAAAATATGT+TGG 0.531217 2_4:+11302510 Msa0315120:CDS
ACTTGCAATGGAACAGATTG+AGG 0.536805 2_4:+11301886 Msa0315120:CDS
TGAAGGAAGAGGTATTGGAT+TGG 0.539165 2_4:+11301946 Msa0315120:CDS
GTAGAAGCCAATGAGGAGTT+GGG 0.539865 2_4:+11302016 Msa0315120:CDS
AAATCAGCTTGCACTTGCAA+TGG 0.545459 2_4:+11301874 Msa0315120:CDS
TTGTTGTGTATGATATATGT+AGG 0.545690 2_4:-11302740 None:intergenic
AGTTATAAGCACGGAGCTTG+TGG 0.552273 2_4:-11301970 None:intergenic
TGACAGGGTGACATTGGAGA+TGG 0.555270 2_4:+11301776 Msa0315120:intron
TGTTATAAGTCTCTGTTAGA+CGG 0.563415 2_4:+11301628 Msa0315120:CDS
GCAAGAATACCTACAATGTG+GGG 0.568021 2_4:+11301589 Msa0315120:exon
TTATAACAGTATGATGTGAA+TGG 0.568571 2_4:-11301613 None:intergenic
TGCAAGAATACCTACAATGT+GGG 0.572772 2_4:+11301588 Msa0315120:exon
ATATACAAGTACTCCCCTAC+CGG 0.574412 2_4:-11301912 None:intergenic
TGGAGAAGATGTTCTTGTGA+GGG 0.578569 2_4:+11301796 Msa0315120:CDS
CGGGATTGAGCATATTGCAA+TGG 0.579779 2_4:+11301648 Msa0315120:CDS
GTATATCTACGCGGACATGA+AGG 0.601774 2_4:+11301929 Msa0315120:CDS
ATGGAACAGATTGAGGCTGC+CGG 0.604204 2_4:+11301893 Msa0315120:CDS
TTGCTTATTTGGTCATAACA+AGG 0.604841 2_4:+11301498 Msa0315120:five_prime_UTR
AATGGGCCACGTCTATGGCA+TGG 0.606179 2_4:+11302629 Msa0315120:CDS
CTACGCGGACATGAAGGAAG+AGG 0.608496 2_4:+11301935 Msa0315120:CDS
ATGGAACTTAACAACGGCAA+TGG 0.608599 2_4:+11302648 Msa0315120:CDS
CTGCAAGAATACCTACAATG+TGG 0.611887 2_4:+11301587 Msa0315120:exon
ATGGAGAAGATGTTCTTGTG+AGG 0.618966 2_4:+11301795 Msa0315120:CDS
AACAGATTGAGGCTGCCGGT+AGG 0.636736 2_4:+11301897 Msa0315120:CDS
GCTACAATAACTCATGTCAG+TGG 0.640830 2_4:+11303043 Msa0315120:three_prime_UTR
AGATTATATTCCAATAAGCA+AGG 0.641533 2_4:-11302998 None:intergenic
CATCCTGTAAGTTATAAGCA+CGG 0.654892 2_4:-11301979 None:intergenic
CGATACCGTAGAAGCCAATG+AGG 0.672536 2_4:+11302009 Msa0315120:CDS
CAAGAATACCTACAATGTGG+GGG 0.672554 2_4:+11301590 Msa0315120:exon
TAAGTTCCATGCCATAGACG+TGG 0.678127 2_4:-11302635 None:intergenic
GACGTGATAAGAGTTAACAA+CGG 0.711055 2_4:-11302570 None:intergenic
TATGGCATGGAACTTAACAA+CGG 0.722153 2_4:+11302642 Msa0315120:CDS
GGATCTGCCAGATGTGACTG+TGG 0.728800 2_4:+11301851 Msa0315120:CDS
GGAGTACTTGTATATCTACG+CGG 0.793030 2_4:+11301920 Msa0315120:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTGTAAAAAATCCTTTA+TGG - chr2_4:11302109-11302128 None:intergenic 15.0%
!!! TGTTTATTAAAGAGATATTT+TGG + chr2_4:11302219-11302238 Msa0315120:intron 15.0%
!!! ATTTTAACTGTTTAAATACA+TGG + chr2_4:11302377-11302396 Msa0315120:intron 15.0%
!!! ATTAATTTATTGTCACTTTT+TGG - chr2_4:11302799-11302818 None:intergenic 15.0%
!! TTATTGTAAAAAATCCTTTA+TGG - chr2_4:11302109-11302128 None:intergenic 15.0%
!!! TGTTTATTAAAGAGATATTT+TGG + chr2_4:11302219-11302238 Msa0315120:intron 15.0%
!!! ATTTTAACTGTTTAAATACA+TGG + chr2_4:11302377-11302396 Msa0315120:intron 15.0%
!!! ATTAATTTATTGTCACTTTT+TGG - chr2_4:11302799-11302818 None:intergenic 15.0%
!! AATATATTAGAATTGCTCAA+TGG + chr2_4:11302081-11302100 Msa0315120:intron 20.0%
!!! TTTTACAATAACATTTTGAG+AGG + chr2_4:11302118-11302137 Msa0315120:intron 20.0%
!! AATATATTAGAATTGCTCAA+TGG + chr2_4:11302081-11302100 Msa0315120:intron 20.0%
!!! TTTTACAATAACATTTTGAG+AGG + chr2_4:11302118-11302137 Msa0315120:intron 20.0%
!!! TTTATAAGTCTCGTTTTTCA+AGG + chr2_4:11301450-11301469 Msa0315120:five_prime_UTR 25.0%
! ACAAGGAGATTAAAACTTAT+TGG + chr2_4:11301515-11301534 Msa0315120:five_prime_UTR 25.0%
! TTATAACAGTATGATGTGAA+TGG - chr2_4:11301616-11301635 None:intergenic 25.0%
! TTGACTATTTGTAATTTGAC+AGG + chr2_4:11301760-11301779 Msa0315120:intron 25.0%
! TGACTATTTGTAATTTGACA+GGG + chr2_4:11301761-11301780 Msa0315120:intron 25.0%
! ACAATGTCAATTTAGTCATT+CGG - chr2_4:11302245-11302264 None:intergenic 25.0%
! ATTGACATTGTTCGATTAAA+TGG + chr2_4:11302254-11302273 Msa0315120:intron 25.0%
! AACAATCCAACAAAATATGT+TGG + chr2_4:11302510-11302529 Msa0315120:CDS 25.0%
! ATTTGATTCTTCAACAGTAT+TGG - chr2_4:11302679-11302698 None:intergenic 25.0%
! GTATGATATATGTAGGTAAT+AGG - chr2_4:11302736-11302755 None:intergenic 25.0%
! TTGTTGTGTATGATATATGT+AGG - chr2_4:11302743-11302762 None:intergenic 25.0%
! TATATCATACACAACAAAAG+AGG + chr2_4:11302746-11302765 Msa0315120:three_prime_UTR 25.0%
! AGATTATATTCCAATAAGCA+AGG - chr2_4:11303001-11303020 None:intergenic 25.0%
! GCAAAAATAATGAAGAAACT+TGG - chr2_4:11303025-11303044 None:intergenic 25.0%
!!! TTTATAAGTCTCGTTTTTCA+AGG + chr2_4:11301450-11301469 Msa0315120:five_prime_UTR 25.0%
! ACAAGGAGATTAAAACTTAT+TGG + chr2_4:11301515-11301534 Msa0315120:five_prime_UTR 25.0%
! TTATAACAGTATGATGTGAA+TGG - chr2_4:11301616-11301635 None:intergenic 25.0%
! TTGACTATTTGTAATTTGAC+AGG + chr2_4:11301760-11301779 Msa0315120:intron 25.0%
! TGACTATTTGTAATTTGACA+GGG + chr2_4:11301761-11301780 Msa0315120:intron 25.0%
! ACAATGTCAATTTAGTCATT+CGG - chr2_4:11302245-11302264 None:intergenic 25.0%
! ATTGACATTGTTCGATTAAA+TGG + chr2_4:11302254-11302273 Msa0315120:intron 25.0%
! AACAATCCAACAAAATATGT+TGG + chr2_4:11302510-11302529 Msa0315120:CDS 25.0%
! ATTTGATTCTTCAACAGTAT+TGG - chr2_4:11302679-11302698 None:intergenic 25.0%
! GTATGATATATGTAGGTAAT+AGG - chr2_4:11302736-11302755 None:intergenic 25.0%
! TTGTTGTGTATGATATATGT+AGG - chr2_4:11302743-11302762 None:intergenic 25.0%
! TATATCATACACAACAAAAG+AGG + chr2_4:11302746-11302765 Msa0315120:three_prime_UTR 25.0%
! AGATTATATTCCAATAAGCA+AGG - chr2_4:11303001-11303020 None:intergenic 25.0%
! GCAAAAATAATGAAGAAACT+TGG - chr2_4:11303025-11303044 None:intergenic 25.0%
! GATACTTCTTGTTGCTTATT+TGG + chr2_4:11301487-11301506 Msa0315120:five_prime_UTR 30.0%
TTGCTTATTTGGTCATAACA+AGG + chr2_4:11301498-11301517 Msa0315120:five_prime_UTR 30.0%
AAGAAAGAGAGATCAATTAG+TGG + chr2_4:11301552-11301571 Msa0315120:five_prime_UTR 30.0%
TGTTATAAGTCTCTGTTAGA+CGG + chr2_4:11301628-11301647 Msa0315120:CDS 30.0%
TGTATATAATACAGTTTCCG+TGG + chr2_4:11301690-11301709 Msa0315120:intron 30.0%
! CACTAAGTGTTGCAAAATAT+AGG - chr2_4:11301721-11301740 None:intergenic 30.0%
! TATTTTGCAACACTTAGTGA+TGG + chr2_4:11301722-11301741 Msa0315120:intron 30.0%
! ATTTTGCAACACTTAGTGAT+GGG + chr2_4:11301723-11301742 Msa0315120:intron 30.0%
TTCAGTCATTGAAACTGTAA+GGG - chr2_4:11302320-11302339 None:intergenic 30.0%
TTTCAGTCATTGAAACTGTA+AGG - chr2_4:11302321-11302340 None:intergenic 30.0%
TAATAGGTAAAAAGAAGGCA+TGG - chr2_4:11302720-11302739 None:intergenic 30.0%
GTAGGTAATAGGTAAAAAGA+AGG - chr2_4:11302725-11302744 None:intergenic 30.0%
AGTGCCAATCATTATAGATA+AGG + chr2_4:11302906-11302925 Msa0315120:three_prime_UTR 30.0%
!! ATGGCCTTATCTATAATGAT+TGG - chr2_4:11302913-11302932 None:intergenic 30.0%
! CCTGAAAATTGTATTGGTAA+TGG - chr2_4:11302932-11302951 None:intergenic 30.0%
! CCATTACCAATACAATTTTC+AGG + chr2_4:11302929-11302948 Msa0315120:three_prime_UTR 30.0%
AGCAAACCTGAAAATTGTAT+TGG - chr2_4:11302938-11302957 None:intergenic 30.0%
CATTGTATTACCTTGCTTAT+TGG + chr2_4:11302988-11303007 Msa0315120:three_prime_UTR 30.0%
! GATACTTCTTGTTGCTTATT+TGG + chr2_4:11301487-11301506 Msa0315120:five_prime_UTR 30.0%
TTGCTTATTTGGTCATAACA+AGG + chr2_4:11301498-11301517 Msa0315120:five_prime_UTR 30.0%
AAGAAAGAGAGATCAATTAG+TGG + chr2_4:11301552-11301571 Msa0315120:five_prime_UTR 30.0%
TGTTATAAGTCTCTGTTAGA+CGG + chr2_4:11301628-11301647 Msa0315120:CDS 30.0%
TGTATATAATACAGTTTCCG+TGG + chr2_4:11301690-11301709 Msa0315120:intron 30.0%
! CACTAAGTGTTGCAAAATAT+AGG - chr2_4:11301721-11301740 None:intergenic 30.0%
! TATTTTGCAACACTTAGTGA+TGG + chr2_4:11301722-11301741 Msa0315120:intron 30.0%
! ATTTTGCAACACTTAGTGAT+GGG + chr2_4:11301723-11301742 Msa0315120:intron 30.0%
TTCAGTCATTGAAACTGTAA+GGG - chr2_4:11302320-11302339 None:intergenic 30.0%
TTTCAGTCATTGAAACTGTA+AGG - chr2_4:11302321-11302340 None:intergenic 30.0%
TAATAGGTAAAAAGAAGGCA+TGG - chr2_4:11302720-11302739 None:intergenic 30.0%
GTAGGTAATAGGTAAAAAGA+AGG - chr2_4:11302725-11302744 None:intergenic 30.0%
AGTGCCAATCATTATAGATA+AGG + chr2_4:11302906-11302925 Msa0315120:three_prime_UTR 30.0%
!! ATGGCCTTATCTATAATGAT+TGG - chr2_4:11302913-11302932 None:intergenic 30.0%
! CCTGAAAATTGTATTGGTAA+TGG - chr2_4:11302932-11302951 None:intergenic 30.0%
! CCATTACCAATACAATTTTC+AGG + chr2_4:11302929-11302948 Msa0315120:three_prime_UTR 30.0%
AGCAAACCTGAAAATTGTAT+TGG - chr2_4:11302938-11302957 None:intergenic 30.0%
CATTGTATTACCTTGCTTAT+TGG + chr2_4:11302988-11303007 Msa0315120:three_prime_UTR 30.0%
!! TTTTCAAGGTTTTTGCTGTC+TGG + chr2_4:11301464-11301483 Msa0315120:five_prime_UTR 35.0%
!! TTTCAAGGTTTTTGCTGTCT+GGG + chr2_4:11301465-11301484 Msa0315120:five_prime_UTR 35.0%
TGCAAGAATACCTACAATGT+GGG + chr2_4:11301588-11301607 Msa0315120:exon 35.0%
GTTATAAGTCTCTGTTAGAC+GGG + chr2_4:11301629-11301648 Msa0315120:CDS 35.0%
TGAGCATATTGCAATGGTTA+AGG + chr2_4:11301654-11301673 Msa0315120:CDS 35.0%
GCAAAATATAGGTAGTTCCA+CGG - chr2_4:11301710-11301729 None:intergenic 35.0%
!!! TTAGTGATGGGTTTTTGACA+AGG + chr2_4:11301735-11301754 Msa0315120:intron 35.0%
TGTCTAACTGGAGACATATT+TGG + chr2_4:11301830-11301849 Msa0315120:CDS 35.0%
CATCCTGTAAGTTATAAGCA+CGG - chr2_4:11301982-11302001 None:intergenic 35.0%
GCTTATAACTTACAGGATGA+TGG + chr2_4:11301983-11302002 Msa0315120:CDS 35.0%
ATTGCTCAATGGTTCCATAA+AGG + chr2_4:11302092-11302111 Msa0315120:intron 35.0%
ATGGAAAGTTATCACAGACA+TGG + chr2_4:11302414-11302433 Msa0315120:intron 35.0%
GAAAAAGTAGCAACATTCCA+AGG - chr2_4:11302446-11302465 None:intergenic 35.0%
!!! TGCTACTTTTTCAGATACTG+AGG + chr2_4:11302454-11302473 Msa0315120:intron 35.0%
!!! GCTACTTTTTCAGATACTGA+GGG + chr2_4:11302455-11302474 Msa0315120:intron 35.0%
! TTGAGGCCAACATATTTTGT+TGG - chr2_4:11302519-11302538 None:intergenic 35.0%
! ACAAAATATGTTGGCCTCAA+AGG + chr2_4:11302519-11302538 Msa0315120:CDS 35.0%
ACGTGATAAGAGTTAACAAC+GGG - chr2_4:11302572-11302591 None:intergenic 35.0%
GACGTGATAAGAGTTAACAA+CGG - chr2_4:11302573-11302592 None:intergenic 35.0%
CTCTAAGTATCTCTTGTTCT+CGG - chr2_4:11302595-11302614 None:intergenic 35.0%
TATGGCATGGAACTTAACAA+CGG + chr2_4:11302642-11302661 Msa0315120:CDS 35.0%
GAAGAATCAAATGTTGTTGC+TGG + chr2_4:11302687-11302706 Msa0315120:CDS 35.0%
! TTGCCTGATTTTCTTCAAGA+AGG + chr2_4:11302850-11302869 Msa0315120:three_prime_UTR 35.0%
!! TTTTCAAGGTTTTTGCTGTC+TGG + chr2_4:11301464-11301483 Msa0315120:five_prime_UTR 35.0%
!! TTTCAAGGTTTTTGCTGTCT+GGG + chr2_4:11301465-11301484 Msa0315120:five_prime_UTR 35.0%
TGCAAGAATACCTACAATGT+GGG + chr2_4:11301588-11301607 Msa0315120:exon 35.0%
GTTATAAGTCTCTGTTAGAC+GGG + chr2_4:11301629-11301648 Msa0315120:CDS 35.0%
TGAGCATATTGCAATGGTTA+AGG + chr2_4:11301654-11301673 Msa0315120:CDS 35.0%
GCAAAATATAGGTAGTTCCA+CGG - chr2_4:11301710-11301729 None:intergenic 35.0%
!!! TTAGTGATGGGTTTTTGACA+AGG + chr2_4:11301735-11301754 Msa0315120:intron 35.0%
TGTCTAACTGGAGACATATT+TGG + chr2_4:11301830-11301849 Msa0315120:CDS 35.0%
CATCCTGTAAGTTATAAGCA+CGG - chr2_4:11301982-11302001 None:intergenic 35.0%
GCTTATAACTTACAGGATGA+TGG + chr2_4:11301983-11302002 Msa0315120:CDS 35.0%
ATTGCTCAATGGTTCCATAA+AGG + chr2_4:11302092-11302111 Msa0315120:intron 35.0%
ATGGAAAGTTATCACAGACA+TGG + chr2_4:11302414-11302433 Msa0315120:intron 35.0%
GAAAAAGTAGCAACATTCCA+AGG - chr2_4:11302446-11302465 None:intergenic 35.0%
!!! TGCTACTTTTTCAGATACTG+AGG + chr2_4:11302454-11302473 Msa0315120:intron 35.0%
!!! GCTACTTTTTCAGATACTGA+GGG + chr2_4:11302455-11302474 Msa0315120:intron 35.0%
! TTGAGGCCAACATATTTTGT+TGG - chr2_4:11302519-11302538 None:intergenic 35.0%
! ACAAAATATGTTGGCCTCAA+AGG + chr2_4:11302519-11302538 Msa0315120:CDS 35.0%
ACGTGATAAGAGTTAACAAC+GGG - chr2_4:11302572-11302591 None:intergenic 35.0%
GACGTGATAAGAGTTAACAA+CGG - chr2_4:11302573-11302592 None:intergenic 35.0%
CTCTAAGTATCTCTTGTTCT+CGG - chr2_4:11302595-11302614 None:intergenic 35.0%
TATGGCATGGAACTTAACAA+CGG + chr2_4:11302642-11302661 Msa0315120:CDS 35.0%
GAAGAATCAAATGTTGTTGC+TGG + chr2_4:11302687-11302706 Msa0315120:CDS 35.0%
! TTGCCTGATTTTCTTCAAGA+AGG + chr2_4:11302850-11302869 Msa0315120:three_prime_UTR 35.0%
CTGCAAGAATACCTACAATG+TGG + chr2_4:11301587-11301606 Msa0315120:exon 40.0%
GCAAGAATACCTACAATGTG+GGG + chr2_4:11301589-11301608 Msa0315120:exon 40.0%
CAAGAATACCTACAATGTGG+GGG + chr2_4:11301590-11301609 Msa0315120:exon 40.0%
GTAATTTGACAGGGTGACAT+TGG + chr2_4:11301770-11301789 Msa0315120:intron 40.0%
ATGGAGAAGATGTTCTTGTG+AGG + chr2_4:11301795-11301814 Msa0315120:CDS 40.0%
TGGAGAAGATGTTCTTGTGA+GGG + chr2_4:11301796-11301815 Msa0315120:CDS 40.0%
AAATCAGCTTGCACTTGCAA+TGG + chr2_4:11301874-11301893 Msa0315120:CDS 40.0%
ACTTGCAATGGAACAGATTG+AGG + chr2_4:11301886-11301905 Msa0315120:CDS 40.0%
ATATACAAGTACTCCCCTAC+CGG - chr2_4:11301915-11301934 None:intergenic 40.0%
! GGAGTACTTGTATATCTACG+CGG + chr2_4:11301920-11301939 Msa0315120:CDS 40.0%
!! TGAAGGAAGAGGTATTGGAT+TGG + chr2_4:11301946-11301965 Msa0315120:CDS 40.0%
!! GAAGGAAGAGGTATTGGATT+GGG + chr2_4:11301947-11301966 Msa0315120:CDS 40.0%
GTCATTGAAACTGTAAGGGT+CGG - chr2_4:11302316-11302335 None:intergenic 40.0%
TTTCAGATACTGAGGGATCT+AGG + chr2_4:11302462-11302481 Msa0315120:intron 40.0%
!! ATGGTTTGACTGTTTCTGGT+AGG + chr2_4:11302544-11302563 Msa0315120:CDS 40.0%
AGAGACCAAACGTGTGAAAA+TGG + chr2_4:11302611-11302630 Msa0315120:CDS 40.0%
GAGACCAAACGTGTGAAAAT+GGG + chr2_4:11302612-11302631 Msa0315120:CDS 40.0%
ATGGAACTTAACAACGGCAA+TGG + chr2_4:11302648-11302667 Msa0315120:CDS 40.0%
GTGCCTTCTTGAAGAAAATC+AGG - chr2_4:11302856-11302875 None:intergenic 40.0%
! CAGGTTTGCTTGTCTTTCTT+TGG + chr2_4:11302948-11302967 Msa0315120:three_prime_UTR 40.0%
!! TTCTTTGGTGTCTGTTACAG+AGG + chr2_4:11302963-11302982 Msa0315120:three_prime_UTR 40.0%
GCTACAATAACTCATGTCAG+TGG + chr2_4:11303043-11303062 Msa0315120:three_prime_UTR 40.0%
CTGCAAGAATACCTACAATG+TGG + chr2_4:11301587-11301606 Msa0315120:exon 40.0%
GCAAGAATACCTACAATGTG+GGG + chr2_4:11301589-11301608 Msa0315120:exon 40.0%
CAAGAATACCTACAATGTGG+GGG + chr2_4:11301590-11301609 Msa0315120:exon 40.0%
GTAATTTGACAGGGTGACAT+TGG + chr2_4:11301770-11301789 Msa0315120:intron 40.0%
ATGGAGAAGATGTTCTTGTG+AGG + chr2_4:11301795-11301814 Msa0315120:CDS 40.0%
TGGAGAAGATGTTCTTGTGA+GGG + chr2_4:11301796-11301815 Msa0315120:CDS 40.0%
AAATCAGCTTGCACTTGCAA+TGG + chr2_4:11301874-11301893 Msa0315120:CDS 40.0%
ACTTGCAATGGAACAGATTG+AGG + chr2_4:11301886-11301905 Msa0315120:CDS 40.0%
ATATACAAGTACTCCCCTAC+CGG - chr2_4:11301915-11301934 None:intergenic 40.0%
! GGAGTACTTGTATATCTACG+CGG + chr2_4:11301920-11301939 Msa0315120:CDS 40.0%
!! TGAAGGAAGAGGTATTGGAT+TGG + chr2_4:11301946-11301965 Msa0315120:CDS 40.0%
!! GAAGGAAGAGGTATTGGATT+GGG + chr2_4:11301947-11301966 Msa0315120:CDS 40.0%
GTCATTGAAACTGTAAGGGT+CGG - chr2_4:11302316-11302335 None:intergenic 40.0%
TTTCAGATACTGAGGGATCT+AGG + chr2_4:11302462-11302481 Msa0315120:intron 40.0%
!! ATGGTTTGACTGTTTCTGGT+AGG + chr2_4:11302544-11302563 Msa0315120:CDS 40.0%
AGAGACCAAACGTGTGAAAA+TGG + chr2_4:11302611-11302630 Msa0315120:CDS 40.0%
GAGACCAAACGTGTGAAAAT+GGG + chr2_4:11302612-11302631 Msa0315120:CDS 40.0%
ATGGAACTTAACAACGGCAA+TGG + chr2_4:11302648-11302667 Msa0315120:CDS 40.0%
GTGCCTTCTTGAAGAAAATC+AGG - chr2_4:11302856-11302875 None:intergenic 40.0%
! CAGGTTTGCTTGTCTTTCTT+TGG + chr2_4:11302948-11302967 Msa0315120:three_prime_UTR 40.0%
!! TTCTTTGGTGTCTGTTACAG+AGG + chr2_4:11302963-11302982 Msa0315120:three_prime_UTR 40.0%
GCTACAATAACTCATGTCAG+TGG + chr2_4:11303043-11303062 Msa0315120:three_prime_UTR 40.0%
CGGGATTGAGCATATTGCAA+TGG + chr2_4:11301648-11301667 Msa0315120:CDS 45.0%
! GTACACTCAGAGTGTCTAAC+TGG + chr2_4:11301818-11301837 Msa0315120:CDS 45.0%
CTGATTTCCACAGTCACATC+TGG - chr2_4:11301861-11301880 None:intergenic 45.0%
GTATATCTACGCGGACATGA+AGG + chr2_4:11301929-11301948 Msa0315120:CDS 45.0%
! GGACATGAAGGAAGAGGTAT+TGG + chr2_4:11301941-11301960 Msa0315120:CDS 45.0%
AGTTATAAGCACGGAGCTTG+TGG - chr2_4:11301973-11301992 None:intergenic 45.0%
GCTCCGTGCTTATAACTTAC+AGG + chr2_4:11301976-11301995 Msa0315120:CDS 45.0%
!! CAACTCCTCATTGGCTTCTA+CGG - chr2_4:11302017-11302036 None:intergenic 45.0%
GTAGAAGCCAATGAGGAGTT+GGG + chr2_4:11302016-11302035 Msa0315120:CDS 45.0%
CCATATTCCCTAGAGTCAAC+AGG - chr2_4:11302046-11302065 None:intergenic 45.0%
!! CCTGTTGACTCTAGGGAATA+TGG + chr2_4:11302043-11302062 Msa0315120:intron 45.0%
! GACTCTAGGGAATATGGCAT+AGG + chr2_4:11302049-11302068 Msa0315120:intron 45.0%
CATGGCGATCACTGTTGTTA+TGG + chr2_4:11302395-11302414 Msa0315120:intron 45.0%
CACAGACATGGCTTAAACCT+TGG + chr2_4:11302426-11302445 Msa0315120:intron 45.0%
! TATGTTGGCCTCAAAGGCTA+TGG + chr2_4:11302525-11302544 Msa0315120:CDS 45.0%
CAGTCAAACCATAGCCTTTG+AGG - chr2_4:11302536-11302555 None:intergenic 45.0%
GGCTATGGTTTGACTGTTTC+TGG + chr2_4:11302540-11302559 Msa0315120:CDS 45.0%
TAAGTTCCATGCCATAGACG+TGG - chr2_4:11302638-11302657 None:intergenic 45.0%
CGGGATTGAGCATATTGCAA+TGG + chr2_4:11301648-11301667 Msa0315120:CDS 45.0%
! GTACACTCAGAGTGTCTAAC+TGG + chr2_4:11301818-11301837 Msa0315120:CDS 45.0%
CTGATTTCCACAGTCACATC+TGG - chr2_4:11301861-11301880 None:intergenic 45.0%
GTATATCTACGCGGACATGA+AGG + chr2_4:11301929-11301948 Msa0315120:CDS 45.0%
! GGACATGAAGGAAGAGGTAT+TGG + chr2_4:11301941-11301960 Msa0315120:CDS 45.0%
AGTTATAAGCACGGAGCTTG+TGG - chr2_4:11301973-11301992 None:intergenic 45.0%
GCTCCGTGCTTATAACTTAC+AGG + chr2_4:11301976-11301995 Msa0315120:CDS 45.0%
!! CAACTCCTCATTGGCTTCTA+CGG - chr2_4:11302017-11302036 None:intergenic 45.0%
GTAGAAGCCAATGAGGAGTT+GGG + chr2_4:11302016-11302035 Msa0315120:CDS 45.0%
CCATATTCCCTAGAGTCAAC+AGG - chr2_4:11302046-11302065 None:intergenic 45.0%
!! CCTGTTGACTCTAGGGAATA+TGG + chr2_4:11302043-11302062 Msa0315120:intron 45.0%
! GACTCTAGGGAATATGGCAT+AGG + chr2_4:11302049-11302068 Msa0315120:intron 45.0%
CATGGCGATCACTGTTGTTA+TGG + chr2_4:11302395-11302414 Msa0315120:intron 45.0%
CACAGACATGGCTTAAACCT+TGG + chr2_4:11302426-11302445 Msa0315120:intron 45.0%
! TATGTTGGCCTCAAAGGCTA+TGG + chr2_4:11302525-11302544 Msa0315120:CDS 45.0%
CAGTCAAACCATAGCCTTTG+AGG - chr2_4:11302536-11302555 None:intergenic 45.0%
GGCTATGGTTTGACTGTTTC+TGG + chr2_4:11302540-11302559 Msa0315120:CDS 45.0%
TAAGTTCCATGCCATAGACG+TGG - chr2_4:11302638-11302657 None:intergenic 45.0%
TGACAGGGTGACATTGGAGA+TGG + chr2_4:11301776-11301795 Msa0315120:intron 50.0%
ATGGAACAGATTGAGGCTGC+CGG + chr2_4:11301893-11301912 Msa0315120:CDS 50.0%
CGATACCGTAGAAGCCAATG+AGG + chr2_4:11302009-11302028 Msa0315120:CDS 50.0%
CGTAGAAGCCAATGAGGAGT+TGG + chr2_4:11302015-11302034 Msa0315120:CDS 50.0%
AGGCAATCCCAACTCCTCAT+TGG - chr2_4:11302026-11302045 None:intergenic 50.0%
!! TGGGATTGCCTGTTGACTCT+AGG + chr2_4:11302035-11302054 Msa0315120:intron 50.0%
!! GGGATTGCCTGTTGACTCTA+GGG + chr2_4:11302036-11302055 Msa0315120:intron 50.0%
AAACTGTAAGGGTCGGTCTG+AGG - chr2_4:11302309-11302328 None:intergenic 50.0%
! GTGGCCCATTTTCACACGTT+TGG - chr2_4:11302619-11302638 None:intergenic 50.0%
GTGAAAATGGGCCACGTCTA+TGG + chr2_4:11302624-11302643 Msa0315120:CDS 50.0%
TGACAGGGTGACATTGGAGA+TGG + chr2_4:11301776-11301795 Msa0315120:intron 50.0%
ATGGAACAGATTGAGGCTGC+CGG + chr2_4:11301893-11301912 Msa0315120:CDS 50.0%
CGATACCGTAGAAGCCAATG+AGG + chr2_4:11302009-11302028 Msa0315120:CDS 50.0%
CGTAGAAGCCAATGAGGAGT+TGG + chr2_4:11302015-11302034 Msa0315120:CDS 50.0%
AGGCAATCCCAACTCCTCAT+TGG - chr2_4:11302026-11302045 None:intergenic 50.0%
!! TGGGATTGCCTGTTGACTCT+AGG + chr2_4:11302035-11302054 Msa0315120:intron 50.0%
!! GGGATTGCCTGTTGACTCTA+GGG + chr2_4:11302036-11302055 Msa0315120:intron 50.0%
AAACTGTAAGGGTCGGTCTG+AGG - chr2_4:11302309-11302328 None:intergenic 50.0%
! GTGGCCCATTTTCACACGTT+TGG - chr2_4:11302619-11302638 None:intergenic 50.0%
GTGAAAATGGGCCACGTCTA+TGG + chr2_4:11302624-11302643 Msa0315120:CDS 50.0%
GTGAATGGCCCCCACATTGT+AGG - chr2_4:11301601-11301620 None:intergenic 55.0%
GGATCTGCCAGATGTGACTG+TGG + chr2_4:11301851-11301870 Msa0315120:CDS 55.0%
AACAGATTGAGGCTGCCGGT+AGG + chr2_4:11301897-11301916 Msa0315120:CDS 55.0%
ACAGATTGAGGCTGCCGGTA+GGG + chr2_4:11301898-11301917 Msa0315120:CDS 55.0%
CTACGCGGACATGAAGGAAG+AGG + chr2_4:11301935-11301954 Msa0315120:CDS 55.0%
GGAATATGGCATAGGCGCAC+AGG + chr2_4:11302057-11302076 Msa0315120:intron 55.0%
AATGGGCCACGTCTATGGCA+TGG + chr2_4:11302629-11302648 Msa0315120:CDS 55.0%
GTGAATGGCCCCCACATTGT+AGG - chr2_4:11301601-11301620 None:intergenic 55.0%
GGATCTGCCAGATGTGACTG+TGG + chr2_4:11301851-11301870 Msa0315120:CDS 55.0%
AACAGATTGAGGCTGCCGGT+AGG + chr2_4:11301897-11301916 Msa0315120:CDS 55.0%
ACAGATTGAGGCTGCCGGTA+GGG + chr2_4:11301898-11301917 Msa0315120:CDS 55.0%
CTACGCGGACATGAAGGAAG+AGG + chr2_4:11301935-11301954 Msa0315120:CDS 55.0%
GGAATATGGCATAGGCGCAC+AGG + chr2_4:11302057-11302076 Msa0315120:intron 55.0%
AATGGGCCACGTCTATGGCA+TGG + chr2_4:11302629-11302648 Msa0315120:CDS 55.0%
CAGATTGAGGCTGCCGGTAG+GGG + chr2_4:11301899-11301918 Msa0315120:CDS 60.0%
CAGATTGAGGCTGCCGGTAG+GGG + chr2_4:11301899-11301918 Msa0315120:CDS 60.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 11301446 11303078 11301446 ID=Msa0315120;Name=Msa0315120
chr2_4 mRNA 11301446 11303078 11301446 ID=Msa0315120-mRNA-1;Parent=Msa0315120;Name=Msa0315120-mRNA-1;_AED=0.06;_eAED=0.06;_QI=158|0.5|0.66|1|1|1|3|362|194
chr2_4 exon 11301446 11301675 11301446 ID=Msa0315120-mRNA-1:exon:4350;Parent=Msa0315120-mRNA-1
chr2_4 exon 11301782 11302045 11301782 ID=Msa0315120-mRNA-1:exon:4351;Parent=Msa0315120-mRNA-1
chr2_4 exon 11302468 11303078 11302468 ID=Msa0315120-mRNA-1:exon:4352;Parent=Msa0315120-mRNA-1
chr2_4 five_prime_UTR 11301446 11301603 11301446 ID=Msa0315120-mRNA-1:five_prime_utr;Parent=Msa0315120-mRNA-1
chr2_4 CDS 11301604 11301675 11301604 ID=Msa0315120-mRNA-1:cds;Parent=Msa0315120-mRNA-1
chr2_4 CDS 11301782 11302045 11301782 ID=Msa0315120-mRNA-1:cds;Parent=Msa0315120-mRNA-1
chr2_4 CDS 11302468 11302716 11302468 ID=Msa0315120-mRNA-1:cds;Parent=Msa0315120-mRNA-1
chr2_4 three_prime_UTR 11302717 11303078 11302717 ID=Msa0315120-mRNA-1:three_prime_utr;Parent=Msa0315120-mRNA-1
Gene Sequence

>Msa0315120

ATGTGGGGGCCATTCACATCATACTGTTATAAGTCTCTGTTAGACGGGATTGAGCATATTGCAATGGTTAAGGGTGACATTGGAGATGGAGAAGATGTTCTTGTGAGGGTACACTCAGAGTGTCTAACTGGAGACATATTTGGATCTGCCAGATGTGACTGTGGAAATCAGCTTGCACTTGCAATGGAACAGATTGAGGCTGCCGGTAGGGGAGTACTTGTATATCTACGCGGACATGAAGGAAGAGGTATTGGATTGGGCCACAAGCTCCGTGCTTATAACTTACAGGATGATGGACGCGATACCGTAGAAGCCAATGAGGAGTTGGGATTGCCTATACTGAGGGATCTAGGCGTTAAATCTATGAAGTTGATGACAAACAATCCAACAAAATATGTTGGCCTCAAAGGCTATGGTTTGACTGTTTCTGGTAGGATCCCGTTGTTAACTCTTATCACGTCCGAGAACAAGAGATACTTAGAGACCAAACGTGTGAAAATGGGCCACGTCTATGGCATGGAACTTAACAACGGCAATGGTAAAACCAATACTGTTGAAGAATCAAATGTTGTTGCTGGCTTTTGA
Protein sequence

>Msa0315120

MWGPFTSYCYKSLLDGIEHIAMVKGDIGDGEDVLVRVHSECLTGDIFGSARCDCGNQLALAMEQIEAAGRGVLVYLRGHEGRGIGLGHKLRAYNLQDDGRDTVEANEELGLPILRDLGVKSMKLMTNNPTKYVGLKGYGLTVSGRIPLLTLITSENKRYLETKRVKMGHVYGMELNNGNGKTNTVEESNVVAGF*