AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0185960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0185960 MtrunA17_Chr1g0192821 95.223 649 24 1 1 649 1 642 0.0 1241
Msa0185960 MtrunA17_Chr4g0061581 35.669 314 178 10 22 321 19 322 3.35e-49 181
Msa0185960 MtrunA17_Chr4g0061581 31.801 261 157 8 72 322 19 268 5.05e-35 140
Msa0185960 MtrunA17_Chr4g0061581 32.000 225 143 5 15 232 65 286 4.39e-31 128
Msa0185960 MtrunA17_Chr7g0272641 33.333 303 179 10 15 310 174 460 2.24e-35 142
Msa0185960 MtrunA17_Chr7g0272641 30.622 209 133 6 11 212 276 479 9.51e-23 103
Msa0185960 MtrunA17_Chr1g0197401 29.787 329 201 11 15 325 15 331 5.67e-33 133
Msa0185960 MtrunA17_Chr1g0198221 29.412 306 185 8 22 306 14 309 6.22e-29 121
Msa0185960 MtrunA17_Chr1g0198221 31.707 246 156 6 71 308 13 254 6.92e-28 117
Msa0185960 MtrunA17_Chr1g0198221 30.403 273 162 7 14 267 57 320 1.90e-26 113
Msa0185960 MtrunA17_Chr1g0198221 30.986 213 132 4 122 328 14 217 4.60e-24 106
Msa0185960 MtrunA17_Chr2g0286241 32.489 237 154 5 14 247 133 366 9.52e-27 115
Msa0185960 MtrunA17_Chr2g0286241 30.909 275 166 9 15 277 76 338 1.07e-26 115
Msa0185960 MtrunA17_Chr2g0286241 27.273 264 176 6 57 312 68 323 2.92e-25 111
Msa0185960 MtrunA17_Chr2g0286241 33.125 160 91 4 174 324 81 233 4.94e-17 85.1
Msa0185960 MtrunA17_Chr2g0286241 26.906 223 138 8 112 322 73 282 9.72e-17 84.3
Msa0185960 MtrunA17_Chr2g0286241 32.692 156 94 4 11 158 232 384 7.01e-14 75.1
Msa0185960 MtrunA17_Chr4g0066851 27.237 257 169 5 65 312 84 331 8.57e-26 112
Msa0185960 MtrunA17_Chr4g0066851 30.712 267 175 6 8 271 135 394 1.00e-25 112
Msa0185960 MtrunA17_Chr4g0066851 29.137 278 167 10 15 277 84 346 3.24e-23 104
Msa0185960 MtrunA17_Chr4g0066851 33.333 162 88 5 174 324 89 241 1.40e-16 84.0
Msa0185960 MtrunA17_Chr4g0074871 29.758 289 179 11 29 306 279 554 5.18e-25 110
Msa0185960 MtrunA17_Chr4g0074871 30.942 223 140 6 89 307 293 505 1.17e-19 94.0
Msa0185960 MtrunA17_Chr4g0074871 31.658 199 131 4 25 222 333 527 2.20e-18 89.7
Msa0185960 MtrunA17_Chr4g0074871 28.492 179 118 5 23 199 384 554 2.45e-14 77.0
Msa0185960 MtrunA17_Chr2g0311721 24.540 326 219 9 15 323 8 323 6.80e-18 85.5
Msa0185960 MtrunA17_Chr2g0297971 31.081 296 163 19 60 328 278 559 5.79e-17 84.7
Msa0185960 MtrunA17_Chr2g0297971 26.239 343 216 16 8 323 276 608 1.32e-13 73.9
Msa0185960 MtrunA17_Chr8g0391901 28.313 332 200 15 22 326 311 631 6.05e-17 84.7
Msa0185960 MtrunA17_Chr8g0391901 28.826 281 164 16 24 280 364 632 1.32e-15 80.5
Msa0185960 MtrunA17_Chr8g0391901 28.231 294 174 16 60 328 298 579 3.69e-15 79.0
Msa0185960 MtrunA17_Chr4g0002171 28.148 270 152 12 25 259 172 434 8.43e-15 77.4
Msa0185960 MtrunA17_Chr4g0002171 28.125 352 190 17 25 322 118 460 6.79e-14 74.3
Msa0185960 MtrunA17_Chr1g0207821 25.692 253 163 8 52 282 100 349 1.01e-14 77.8
Msa0185960 MtrunA17_Chr1g0196221 28.800 250 157 7 15 254 86 324 3.22e-14 76.3
Msa0185960 MtrunA17_Chr1g0196221 26.573 286 168 13 70 324 22 296 9.85e-14 74.7
Msa0185960 MtrunA17_Chr2g0322121 28.319 226 119 9 125 314 81 299 7.33e-12 68.9
Msa0185960 MtrunA17_Chr2g0322121 26.855 283 169 11 53 306 142 415 8.75e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0185960 AT1G74150.3 36.223 323 184 9 23 332 17 330 3.97e-53 192
Msa0185960 AT1G74150.3 31.119 286 178 8 14 291 61 335 3.27e-31 129
Msa0185960 AT1G74150.2 36.278 317 180 9 23 326 17 324 7.52e-53 191
Msa0185960 AT1G74150.2 30.824 279 160 11 63 322 1 265 1.63e-34 139
Msa0185960 AT1G74150.2 32.271 251 155 7 14 256 61 304 6.05e-31 128
Msa0185960 AT1G74150.1 36.278 317 180 9 23 326 17 324 7.56e-53 191
Msa0185960 AT1G74150.1 30.824 279 160 11 63 322 1 265 1.57e-34 139
Msa0185960 AT1G74150.1 32.271 251 155 7 14 256 61 304 5.30e-31 128
Msa0185960 AT1G18610.1 36.129 310 176 9 25 321 22 322 1.82e-52 190
Msa0185960 AT1G18610.1 30.466 279 164 10 63 322 1 268 2.93e-31 129
Msa0185960 AT1G18610.1 31.746 252 159 6 12 256 62 307 2.93e-31 129
Msa0185960 AT1G18610.2 36.129 310 176 9 25 321 22 322 1.82e-52 190
Msa0185960 AT1G18610.2 30.466 279 164 10 63 322 1 268 2.93e-31 129
Msa0185960 AT1G18610.2 31.746 252 159 6 12 256 62 307 2.93e-31 129
Msa0185960 AT1G74150.4 37.453 267 148 7 65 321 46 303 2.37e-45 170
Msa0185960 AT1G74150.4 32.061 262 159 8 3 256 38 288 2.44e-31 129
Msa0185960 AT1G74150.4 31.193 218 132 7 112 322 43 249 1.55e-29 124
Msa0185960 AT3G05420.1 32.362 309 182 9 15 306 171 469 2.27e-36 145
Msa0185960 AT3G05420.1 26.937 271 177 8 64 323 170 430 1.18e-23 106
Msa0185960 AT3G05420.1 30.263 152 98 4 15 161 333 481 2.18e-13 73.9
Msa0185960 AT3G05420.2 32.362 309 182 9 15 306 172 470 2.33e-36 145
Msa0185960 AT3G05420.2 26.937 271 177 8 64 323 171 431 1.17e-23 106
Msa0185960 AT3G05420.2 30.263 152 98 4 15 161 334 482 2.15e-13 73.9
Msa0185960 AT5G27630.3 29.314 423 254 12 15 421 172 565 6.49e-35 141
Msa0185960 AT5G27630.1 29.323 399 242 13 15 396 172 547 9.60e-35 140
Msa0185960 AT5G27630.4 33.818 275 160 7 15 277 172 436 1.47e-34 140
Msa0185960 AT5G27630.2 30.295 373 231 11 15 371 172 531 2.28e-34 138
Msa0185960 AT5G04420.2 28.529 340 204 12 15 327 22 349 6.84e-28 118
Msa0185960 AT5G04420.2 27.653 311 178 11 64 340 21 318 2.89e-22 101
Msa0185960 AT5G04420.2 30.323 155 99 5 25 174 201 351 2.57e-11 67.0
Msa0185960 AT5G04420.1 28.529 340 204 12 15 327 22 349 6.84e-28 118
Msa0185960 AT5G04420.1 27.653 311 178 11 64 340 21 318 2.89e-22 101
Msa0185960 AT5G04420.1 30.323 155 99 5 25 174 201 351 2.57e-11 67.0
Msa0185960 AT5G04420.3 28.529 340 204 12 15 327 22 349 6.84e-28 118
Msa0185960 AT5G04420.3 27.653 311 178 11 64 340 21 318 2.89e-22 101
Msa0185960 AT5G04420.3 30.323 155 99 5 25 174 201 351 2.57e-11 67.0
Msa0185960 AT2G36360.2 28.431 306 188 8 22 306 14 309 2.37e-27 116
Msa0185960 AT2G36360.2 31.707 246 156 6 71 308 13 254 1.11e-26 114
Msa0185960 AT2G36360.2 31.502 273 159 7 14 267 57 320 2.42e-26 113
Msa0185960 AT2G36360.1 28.431 306 188 8 22 306 14 309 2.37e-27 116
Msa0185960 AT2G36360.1 31.707 246 156 6 71 308 13 254 1.11e-26 114
Msa0185960 AT2G36360.1 31.502 273 159 7 14 267 57 320 2.42e-26 113
Msa0185960 AT2G36360.3 28.431 306 188 8 22 306 14 309 2.52e-27 116
Msa0185960 AT2G36360.3 31.707 246 156 6 71 308 13 254 1.11e-26 114
Msa0185960 AT2G36360.3 31.502 273 159 7 14 267 57 320 2.59e-26 113
Msa0185960 AT2G36360.4 28.431 306 188 8 22 306 14 309 3.94e-27 116
Msa0185960 AT2G36360.4 31.707 246 156 6 71 308 13 254 1.66e-26 114
Msa0185960 AT2G36360.4 31.136 273 160 7 14 267 57 320 3.14e-26 113
Msa0185960 AT5G18590.2 27.509 269 179 6 65 325 66 326 8.77e-26 113
Msa0185960 AT5G18590.2 32.212 208 136 4 14 219 123 327 8.45e-24 107
Msa0185960 AT5G18590.2 28.700 223 134 9 112 322 63 272 9.84e-18 87.8
Msa0185960 AT5G18590.1 27.509 269 179 6 65 325 66 326 8.77e-26 113
Msa0185960 AT5G18590.1 32.212 208 136 4 14 219 123 327 8.45e-24 107
Msa0185960 AT5G18590.1 28.700 223 134 9 112 322 63 272 9.84e-18 87.8
Msa0185960 AT2G36360.5 27.937 315 187 9 22 306 14 318 2.03e-25 110
Msa0185960 AT2G36360.5 30.980 255 155 7 71 308 13 263 1.16e-24 108
Msa0185960 AT2G36360.5 30.496 282 159 8 14 267 57 329 9.72e-24 105
Msa0185960 AT4G04670.1 27.541 305 192 7 125 427 322 599 2.49e-25 112
Msa0185960 AT4G04670.1 27.801 241 162 5 67 306 314 543 7.18e-24 107
Msa0185960 AT4G04670.1 29.876 241 155 5 19 256 314 543 2.61e-22 102
Msa0185960 AT4G04670.1 29.570 186 125 4 15 199 363 543 3.09e-11 67.4
Msa0185960 AT5G48180.1 25.617 324 206 13 15 318 8 316 8.05e-20 91.7
Msa0185960 AT5G48180.1 27.237 257 169 8 64 318 7 247 1.44e-15 79.0
Msa0185960 AT1G68050.1 29.275 345 204 15 8 326 285 615 4.86e-18 88.6
Msa0185960 AT3G07720.1 25.506 247 167 6 64 306 9 242 1.02e-16 82.4
Msa0185960 AT3G07720.1 23.724 333 226 11 5 320 1 322 5.43e-15 77.0
Msa0185960 AT3G07720.1 25.455 275 183 9 15 275 62 328 1.21e-13 73.2
Msa0185960 AT1G74150.6 33.113 151 88 4 176 320 9 152 1.75e-16 82.4
Msa0185960 AT1G74150.6 31.667 180 109 9 115 291 2 170 8.84e-14 74.3
Msa0185960 AT1G74150.5 33.113 151 88 4 176 320 9 152 1.75e-16 82.4
Msa0185960 AT1G74150.5 31.667 180 109 9 115 291 2 170 8.84e-14 74.3
Msa0185960 AT1G51550.1 30.714 280 151 17 17 259 166 439 2.15e-16 82.8
Msa0185960 AT1G51550.1 27.244 312 178 17 25 305 120 413 5.69e-14 75.1
Msa0185960 AT5G57360.1 28.696 345 207 18 8 324 273 606 1.09e-15 81.3
Msa0185960 AT2G18915.1 27.168 346 207 15 8 323 264 594 2.43e-15 80.1
Msa0185960 AT2G18915.2 27.168 346 207 15 8 323 274 604 2.68e-15 79.7
Msa0185960 AT2G33070.1 27.236 246 164 6 64 306 153 386 2.65e-14 76.3
Msa0185960 AT2G33070.1 25.229 218 145 5 37 242 229 440 1.84e-13 73.6
Msa0185960 AT2G33070.2 27.236 246 164 6 64 306 153 386 2.65e-14 76.3
Msa0185960 AT2G33070.2 25.229 218 145 5 37 242 229 440 1.84e-13 73.6
Msa0185960 AT5G50310.1 25.000 272 171 9 35 285 92 351 5.78e-14 75.9
Msa0185960 AT5G50310.1 26.866 201 124 7 31 211 128 325 6.27e-11 65.9
Msa0185960 AT5G57360.2 28.135 327 196 18 8 306 273 588 1.11e-13 74.7
Msa0185960 AT2G33070.3 25.229 218 145 5 37 242 231 442 2.21e-13 73.6
Msa0185960 AT2G33070.3 27.016 248 164 7 64 306 153 388 6.19e-13 72.0
Msa0185960 AT2G27210.1 26.429 280 185 10 53 320 152 422 5.12e-13 73.2
Msa0185960 AT3G16400.2 25.118 211 138 4 31 226 216 421 7.70e-13 71.6
Msa0185960 AT3G16400.2 25.826 333 216 12 5 318 143 463 1.76e-12 70.5
Msa0185960 AT3G16400.1 25.118 211 138 4 31 226 216 421 7.70e-13 71.6
Msa0185960 AT3G16400.1 25.826 333 216 12 5 318 143 463 1.76e-12 70.5
Msa0185960 AT1G03445.1 29.348 184 104 8 125 290 28 203 1.66e-12 71.2
Msa0185960 AT1G03445.1 26.936 297 179 13 45 321 71 349 2.98e-11 67.4
Msa0185960 AT1G03445.1 27.106 273 169 11 15 268 86 347 5.39e-11 66.2
Msa0185960 AT1G08420.1 26.071 280 186 10 53 320 163 433 1.70e-12 71.2
Msa0185960 AT3G16410.1 25.126 199 135 3 37 226 377 570 1.84e-12 70.9
Msa0185960 AT3G16410.1 24.898 245 171 5 64 306 301 534 5.28e-11 66.2
Msa0185960 AT3G16410.1 25.430 291 201 9 5 287 292 574 6.50e-11 65.9
Msa0185960 AT1G03445.2 29.348 184 104 8 125 290 28 203 1.87e-12 71.2
Msa0185960 AT1G03445.2 26.936 297 179 13 45 321 71 349 3.71e-11 67.0
Msa0185960 AT1G03445.2 27.106 273 169 11 15 268 86 347 6.37e-11 66.2
Msa0185960 AT4G03080.1 26.718 262 155 11 73 307 25 276 2.03e-12 70.9
Msa0185960 AT3G16390.2 25.110 227 149 5 31 242 216 436 8.54e-12 68.2
Msa0185960 AT3G16390.2 27.600 250 156 8 65 306 153 385 1.06e-11 68.2
Msa0185960 AT3G16390.2 25.672 335 219 13 5 322 143 464 6.36e-11 65.5
Msa0185960 AT3G16390.1 25.110 227 149 5 31 242 216 436 8.54e-12 68.2
Msa0185960 AT3G16390.1 27.600 250 156 8 65 306 153 385 1.06e-11 68.2
Msa0185960 AT3G16390.1 25.672 335 219 13 5 322 143 464 6.36e-11 65.5
Msa0185960 AT2G27210.2 25.993 277 185 10 53 320 152 417 1.54e-11 68.2
Msa0185960 AT1G08420.2 25.632 277 186 10 53 320 163 428 4.53e-11 66.6
Msa0185960 AT1G54040.2 26.562 256 171 9 64 306 8 259 9.25e-11 64.3

Find 165 sgRNAs with CRISPR-Local

Find 248 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGACATACACACTTGTTTA+TGG 0.163401 2_1:+588273 Msa0185960:CDS
TGTGATTGCGAATCTTAATT+TGG 0.221271 2_1:+590242 Msa0185960:three_prime_UTR
GAGCAAGTAGTGGTTGCTTT+AGG 0.236125 2_1:-589828 None:intergenic
CTTGATGGAAACAAACAATT+TGG 0.239299 2_1:-589718 None:intergenic
GGTGGTATCCCTTCACATTT+AGG 0.241948 2_1:-587287 None:intergenic
ATGTGGAAGTCTTGGGAAAA+TGG 0.260192 2_1:+588852 Msa0185960:CDS
AACGTTATTGCAACGACATT+TGG 0.261258 2_1:+588246 Msa0185960:CDS
ATGTTTGGACCTTGACAAAA+TGG 0.261385 2_1:+587103 Msa0185960:CDS
TTTAGGCTTAATCCATTCTT+TGG 0.268713 2_1:-587270 None:intergenic
ATTGATGGAGCCTTTGATTC+AGG 0.269269 2_1:+589529 Msa0185960:CDS
AGCCACATTGCTTTCTTCTT+AGG 0.272073 2_1:-586497 None:intergenic
AAATATTGAGGAGGTTAATA+AGG 0.274112 2_1:+589983 Msa0185960:three_prime_UTR
GGTCACTCTGCTTGCTTCTC+TGG 0.304115 2_1:+586560 Msa0185960:CDS
CGACCGTCTCCAATCATAAA+AGG 0.307200 2_1:+589850 Msa0185960:CDS
CGACCGTGTCAATTTGATTC+AGG 0.313607 2_1:+588902 Msa0185960:CDS
AGTCATTGTACTCTTGCTAT+TGG 0.314706 2_1:+587485 Msa0185960:CDS
GCAAAGTCTTCGGAAGTTAT+TGG 0.321726 2_1:+588606 Msa0185960:CDS
GACACCGATATTGTTATATA+TGG 0.328819 2_1:+588359 Msa0185960:CDS
GGAAATAATGCTTGATCCTT+AGG 0.346867 2_1:+589958 Msa0185960:exon
CAAGCATTATTTCCACTTGC+AGG 0.349704 2_1:-589949 None:intergenic
CAGGAGTGACTTGCAAGGTT+TGG 0.352406 2_1:+589909 Msa0185960:CDS
GTCATTGTACTCTTGCTATT+GGG 0.354949 2_1:+587486 Msa0185960:CDS
GAAGTTCTTGGCAAAATTGA+TGG 0.359155 2_1:+589514 Msa0185960:CDS
AGCAAGTAGTGGTTGCTTTA+GGG 0.360149 2_1:-589827 None:intergenic
TAGATTGGTACCGAAGTTCT+TGG 0.360287 2_1:+589502 Msa0185960:intron
TGTGGAAGTCTTGGGAAAAT+GGG 0.379749 2_1:+588853 Msa0185960:CDS
ACCAGAAAATTTCGCGTATC+GGG 0.384937 2_1:+588825 Msa0185960:intron
ACGTTATTGCAACGACATTT+GGG 0.386709 2_1:+588247 Msa0185960:CDS
AAGGTTCTTCACCAGGAGTC+AGG 0.389468 2_1:+587787 Msa0185960:CDS
AAATCCATGGCAGAAGCATT+TGG 0.396548 2_1:-589133 None:intergenic
AAATGGACATAACAACTTAT+CGG 0.398694 2_1:+588580 Msa0185960:CDS
TTATTTCAGGTATACATCAT+TGG 0.403078 2_1:+588209 Msa0185960:intron
AATGGACATAACAACTTATC+GGG 0.405221 2_1:+588581 Msa0185960:CDS
AACTCCATCTCATAGTTCTA+AGG 0.405995 2_1:-587421 None:intergenic
AATACTCCACATATTATTCA+TGG 0.408013 2_1:+586454 Msa0185960:exon
GATAAGTTGTTATGTCCATT+TGG 0.409080 2_1:-588578 None:intergenic
TGAAGATATTGTTCTTGCTT+TGG 0.410809 2_1:-589292 None:intergenic
CGGGAATGATGTGGAAGTCT+TGG 0.417836 2_1:+588844 Msa0185960:CDS
AAAATATTCAAAATGTCCTT+AGG 0.427856 2_1:+590200 Msa0185960:three_prime_UTR
TTGATCTTTACACCAAAGAA+TGG 0.429246 2_1:+587258 Msa0185960:CDS
CAAGGTTTGGCTTTAACACT+AGG 0.430586 2_1:+589922 Msa0185960:CDS
GGGAATAAGCTTATTGTGTA+TGG 0.432274 2_1:+587506 Msa0185960:CDS
AGGATTAAAACCTAAAACCT+TGG 0.432402 2_1:-586525 None:intergenic
TATCCGCGTCCGGCAGGCTT+CGG 0.433353 2_1:-589358 None:intergenic
GCATGACCTGCCCTGACTCC+TGG 0.433921 2_1:-587798 None:intergenic
AAAGAAGAAGAGATGAAGAA+AGG 0.439452 2_1:-586284 None:intergenic
CTAATGACTGCAGTAGTGAA+TGG 0.440066 2_1:+589556 Msa0185960:CDS
TTCATGAGAGGCTGACTTGA+TGG 0.443717 2_1:-589733 None:intergenic
GTCTTGGGAAAATGGGGTTA+TGG 0.445343 2_1:+588860 Msa0185960:CDS
CAGGCTTCGGTTCTAAAGCT+TGG 0.446872 2_1:-589345 None:intergenic
CCCGCCATATATAACAATAT+CGG 0.451213 2_1:-588363 None:intergenic
CGATATCGCCGAGTCAAACT+AGG 0.452268 2_1:+589160 Msa0185960:CDS
TGAGTGTATCCGCGTCCGGC+AGG 0.454398 2_1:-589364 None:intergenic
TGGAGCAAGGTTACTACAAC+AGG 0.454699 2_1:+587128 Msa0185960:CDS
TTCAACATCCCACACCTTTG+TGG 0.462742 2_1:-589026 None:intergenic
GATCCTTAGGTGAAATATTG+AGG 0.465858 2_1:+589971 Msa0185960:exon
CATGTTAATGATGAAGAGCT+TGG 0.468164 2_1:+590168 Msa0185960:three_prime_UTR
TCAACAAGATCTTCATGTAA+TGG 0.469515 2_1:+589261 Msa0185960:CDS
GGGAATGATGTGGAAGTCTT+GGG 0.474138 2_1:+588845 Msa0185960:CDS
TTATCGGGGTGCAAAGTCTT+CGG 0.475381 2_1:+588596 Msa0185960:CDS
TACCCGGAGAACATAAAATC+AGG 0.479502 2_1:+589890 Msa0185960:CDS
TATCAGTAGACATGAAAAGA+TGG 0.480772 2_1:-586231 None:intergenic
CAATGACTATCTCTAGGAAC+AGG 0.483951 2_1:-587470 None:intergenic
ATGGAGATGAATGCTGAGAC+AGG 0.490805 2_1:-589075 None:intergenic
ATATCTAAGTTACCGTTGTA+TGG 0.491841 2_1:-590115 None:intergenic
TAATCAAATGTTCCATCCTA+AGG 0.495488 2_1:-590216 None:intergenic
GTGTTGAGTGTATCCGCGTC+CGG 0.503593 2_1:-589368 None:intergenic
GTCCTACAAGAACAGCACTA+TGG 0.503973 2_1:-587175 None:intergenic
CTTGCAGCAGAGCATCCAAA+TGG 0.505763 2_1:+588563 Msa0185960:CDS
CCTCCTCAATATTTCACCTA+AGG 0.507116 2_1:-589974 None:intergenic
TAATGACTGCAGTAGTGAAT+GGG 0.507894 2_1:+589557 Msa0185960:CDS
TGAATAATCTATCAGGGAGC+AGG 0.510071 2_1:+589685 Msa0185960:intron
AGCCATAGTGCTGTTCTTGT+AGG 0.510145 2_1:+587173 Msa0185960:CDS
TCAAGAAAAGAAGACCATCC+TGG 0.511840 2_1:+588631 Msa0185960:CDS
GGATTAAAACCTAAAACCTT+GGG 0.516565 2_1:-586524 None:intergenic
GACCAGAAAATTTCGCGTAT+CGG 0.516872 2_1:+588824 Msa0185960:intron
TTCAGGTATACATCATTGGA+GGG 0.528619 2_1:+588213 Msa0185960:intron
TGAAACAATGACTTGGACTA+GGG 0.529748 2_1:+587577 Msa0185960:CDS
TATTCAAAATGTCCTTAGGA+TGG 0.529866 2_1:+590204 Msa0185960:three_prime_UTR
TCTTGTTGGTGGTGAAAGAC+TGG 0.530082 2_1:+587337 Msa0185960:CDS
AACACTAACAATTCATTCAA+AGG 0.530492 2_1:-588536 None:intergenic
CAAATCATGTGGATAATTCG+AGG 0.531788 2_1:+589758 Msa0185960:CDS
TTGTCAAGTTGACAAGTGCT+TGG 0.532756 2_1:-589193 None:intergenic
GAACCGAAGCCTGCCGGACG+CGG 0.532959 2_1:+589355 Msa0185960:CDS
TTTCAGGTATACATCATTGG+AGG 0.533107 2_1:+588212 Msa0185960:intron
TGGTCTCAACTTGATATACA+TGG 0.535726 2_1:+588293 Msa0185960:CDS
TTGTATCTACTCCGCCATCC+AGG 0.538216 2_1:-588649 None:intergenic
CCGATATTGTTATATATGGC+GGG 0.538845 2_1:+588363 Msa0185960:CDS
AGTCACACTGCTACTCTTGT+TGG 0.540157 2_1:+587323 Msa0185960:CDS
GGTCATGCAGCTGTGAATGT+TGG 0.541807 2_1:+587813 Msa0185960:CDS
ATTGTGTATGGTGGAGATAG+TGG 0.545972 2_1:+587518 Msa0185960:CDS
CTCTGCTGTGATTGTGAATG+TGG 0.547630 2_1:-586400 None:intergenic
CAAGAACAATATCTTCATGT+TGG 0.548229 2_1:+589298 Msa0185960:CDS
TGGATTAAGCCTAAATGTGA+AGG 0.551727 2_1:+587278 Msa0185960:CDS
TGTTCTTGATCAGATTGAGA+TGG 0.553752 2_1:-589094 None:intergenic
GCTTTAGAACCGAAGCCTGC+CGG 0.554277 2_1:+589349 Msa0185960:CDS
CAAGCTCTTCATCATTAACA+TGG 0.554718 2_1:-590167 None:intergenic
TCTGATCAAGAACAGACTCC+AGG 0.556028 2_1:+589103 Msa0185960:CDS
GTAGCAGTGTGACTTTCCCT+TGG 0.559880 2_1:-587314 None:intergenic
GTATACATCATTGGAGGGGT+CGG 0.560007 2_1:+588218 Msa0185960:CDS
CATTAGAAAACCTGAATCAA+AGG 0.560029 2_1:-589539 None:intergenic
ACACAATACTACATAACTCA+TGG 0.560215 2_1:-590052 None:intergenic
AAAACCAAATGCTTCTGCCA+TGG 0.560581 2_1:+589129 Msa0185960:CDS
TTGAAACAATGACTTGGACT+AGG 0.562915 2_1:+587576 Msa0185960:CDS
ACCGATATTGTTATATATGG+CGG 0.564182 2_1:+588362 Msa0185960:CDS
GGCACATCACCTTTAACCTC+AGG 0.564358 2_1:-587449 None:intergenic
CTAAGTTACCGTTGTATGGT+CGG 0.565702 2_1:-590111 None:intergenic
GAGACTCCATGAATAATATG+TGG 0.566165 2_1:-586460 None:intergenic
TGACTTTCCCTTGGTGAAGG+TGG 0.566581 2_1:-587305 None:intergenic
AGCTGTGAATGTTGGAACTA+AGG 0.566757 2_1:+587821 Msa0185960:CDS
AGAGTACAATGACTATCTCT+AGG 0.569296 2_1:-587476 None:intergenic
GTGTGACTTTCCCTTGGTGA+AGG 0.574494 2_1:-587308 None:intergenic
ACACTAGGAGGGCCTGCAAG+TGG 0.574524 2_1:+589937 Msa0185960:CDS
AATAAGCTTATTGTGTATGG+TGG 0.576597 2_1:+587509 Msa0185960:CDS
AGGTTCTTCACCAGGAGTCA+GGG 0.576841 2_1:+587788 Msa0185960:CDS
TTGATATTGAAACAATGACT+TGG 0.577584 2_1:+587570 Msa0185960:CDS
GAGTGACCCCATCTTTGAGA+AGG 0.578017 2_1:-586545 None:intergenic
GGTTTGGCTTTAACACTAGG+AGG 0.578223 2_1:+589925 Msa0185960:CDS
CACACTGCTACTCTTGTTGG+TGG 0.578346 2_1:+587326 Msa0185960:CDS
TAGACCTTAGAACTATGAGA+TGG 0.585997 2_1:+587417 Msa0185960:CDS
ATCAGTAGACATGAAAAGAT+GGG 0.587480 2_1:-586230 None:intergenic
TGGACCTTGACAAAATGGAA+TGG 0.587543 2_1:+587108 Msa0185960:CDS
GTGGAAGTCTTGGGAAAATG+GGG 0.591772 2_1:+588854 Msa0185960:CDS
TCTTCACCAGGAGTCAGGGC+AGG 0.593035 2_1:+587792 Msa0185960:CDS
GAATTGCTGCCACAAAGGTG+TGG 0.593293 2_1:+589017 Msa0185960:CDS
CTATGGCTATCTCTAGGACC+TGG 0.596063 2_1:-587158 None:intergenic
TTTAATCCTTCTCAAAGATG+GGG 0.598086 2_1:+586539 Msa0185960:CDS
GCGATATCGCTGAAAATCCA+TGG 0.598217 2_1:-589146 None:intergenic
GAAAAGAAGACCATCCTGGA+TGG 0.600096 2_1:+588635 Msa0185960:CDS
GTTTGGCTTTAACACTAGGA+GGG 0.600809 2_1:+589926 Msa0185960:CDS
ACAGCACTATGGCTATCTCT+AGG 0.602607 2_1:-587164 None:intergenic
ACGCGGATACACTCAACACT+TGG 0.606240 2_1:+589372 Msa0185960:CDS
ATCTACTCCGCCATCCAGGA+TGG 0.609038 2_1:-588645 None:intergenic
GAACTTCACAACAGAAGAGG+AGG 0.609239 2_1:+588993 Msa0185960:CDS
GATAGTGGTGATCAGTATCA+TGG 0.612803 2_1:+587533 Msa0185960:CDS
TTCGCGTATCGGGAATGATG+TGG 0.613683 2_1:+588835 Msa0185960:CDS
CAGGAACTTCACAACAGAAG+AGG 0.614241 2_1:+588990 Msa0185960:intron
ACATGATTTGAGTTCATGAG+AGG 0.614402 2_1:-589745 None:intergenic
ACTACAACAGGTGAAAAGCC+AGG 0.619726 2_1:+587140 Msa0185960:CDS
TCTGACTTGTAGCAGGTGTG+GGG 0.622977 2_1:+588502 Msa0185960:intron
TCTCATGAACTCAAATCATG+TGG 0.625132 2_1:+589747 Msa0185960:CDS
AGCAATGTGGCTGTATCCCA+AGG 0.627800 2_1:+586508 Msa0185960:CDS
GGATTAAGCCTAAATGTGAA+GGG 0.630444 2_1:+587279 Msa0185960:CDS
TCAGGTATACATCATTGGAG+GGG 0.630931 2_1:+588214 Msa0185960:intron
GAGGAGAATTGCTGCCACAA+AGG 0.632549 2_1:+589012 Msa0185960:CDS
TGACAAAATGGAATGGAGCA+AGG 0.633271 2_1:+587115 Msa0185960:CDS
AATTGCTGCCACAAAGGTGT+GGG 0.636773 2_1:+589018 Msa0185960:CDS
GGTTGCTTTAGGGCATAGCA+CGG 0.638751 2_1:-589817 None:intergenic
GGAGACGGTCGAGCAAGTAG+TGG 0.648193 2_1:-589838 None:intergenic
AAGAAGACCATCCTGGATGG+CGG 0.653485 2_1:+588638 Msa0185960:CDS
AGGGATACCACCTTCACCAA+GGG 0.655838 2_1:+587298 Msa0185960:CDS
TGGAGATGAATGCTGAGACA+GGG 0.656111 2_1:-589074 None:intergenic
AAAATCAGGAGTGACTTGCA+AGG 0.656845 2_1:+589904 Msa0185960:CDS
CGTATCTGCCGACCATACAA+CGG 0.661203 2_1:+590103 Msa0185960:three_prime_UTR
CTACCTGAATCAAATTGACA+CGG 0.661599 2_1:-588905 None:intergenic
CCTTAGGTGAAATATTGAGG+AGG 0.666374 2_1:+589974 Msa0185960:three_prime_UTR
ACTAATACAGTGTCTTTACC+CGG 0.667635 2_1:+589874 Msa0185960:CDS
GTATGAGAAAACCGTCCTTG+TGG 0.675141 2_1:-588323 None:intergenic
AAGGGATACCACCTTCACCA+AGG 0.676653 2_1:+587297 Msa0185960:CDS
ATGGTCAACAGCCACAAGGA+CGG 0.677236 2_1:+588312 Msa0185960:CDS
ATCGGTGTCTGCAACAACAG+CGG 0.678886 2_1:-588345 None:intergenic
ATACATGGTCAACAGCCACA+AGG 0.682432 2_1:+588308 Msa0185960:CDS
ATGGACATAACAACTTATCG+GGG 0.694887 2_1:+588582 Msa0185960:CDS
CTCCTAAGAAGAAAGCAATG+TGG 0.723011 2_1:+586495 Msa0185960:CDS
TGGCGGAGTAGATACAAACA+CGG 0.767759 2_1:+588655 Msa0185960:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTGAAAATAATGATTTT+TGG - chr2_1:587662-587681 None:intergenic 10.0%
!!! AATCTTATAATTTGTTCATA+TGG + chr2_1:587620-587639 Msa0185960:intron 15.0%
!! TATATAGCATAAGTAATTAA+CGG - chr2_1:588150-588169 None:intergenic 15.0%
!! TTAATTACTTATGCTATATA+TGG + chr2_1:588150-588169 Msa0185960:intron 15.0%
!!! GTTTATTTTTCTCTTTTTTT+CGG + chr2_1:590073-590092 Msa0185960:three_prime_UTR 15.0%
!!! GAAGACTTATGTATATTTTT+GGG + chr2_1:586582-586601 Msa0185960:CDS 20.0%
!!! AAGACTTATGTATATTTTTG+GGG + chr2_1:586583-586602 Msa0185960:CDS 20.0%
!! TAAGATAGCAAAAATATGAT+TGG - chr2_1:586943-586962 None:intergenic 20.0%
!!! ATAATTTGTTCATATGGAAA+TGG + chr2_1:587626-587645 Msa0185960:intron 20.0%
!!! TATGAATTTAGGAGTATTTT+AGG - chr2_1:587919-587938 None:intergenic 20.0%
!!! TATTTATAGGAATTTGTTCT+AGG - chr2_1:587974-587993 None:intergenic 20.0%
!!! TTCTCAAAAGTGTTATTTAT+AGG - chr2_1:587987-588006 None:intergenic 20.0%
!!! AAAGAACTAATAATGTGTTT+TGG + chr2_1:588802-588821 Msa0185960:intron 20.0%
!! TTAAATTTGTAATGTTATGC+AGG + chr2_1:588971-588990 Msa0185960:intron 20.0%
!!! AATTTGAATTTTGAAAGCAA+AGG - chr2_1:589403-589422 None:intergenic 20.0%
!! TAACAAATGAATAATCTATC+AGG + chr2_1:589678-589697 Msa0185960:intron 20.0%
!! AACAAATGAATAATCTATCA+GGG + chr2_1:589679-589698 Msa0185960:intron 20.0%
!! AAAATATTCAAAATGTCCTT+AGG + chr2_1:590200-590219 Msa0185960:three_prime_UTR 20.0%
! AATACTCCACATATTATTCA+TGG + chr2_1:586454-586473 Msa0185960:exon 25.0%
!! TTTTAATCCTTCTCAAAGAT+GGG + chr2_1:586538-586557 Msa0185960:CDS 25.0%
!! GGAAGACTTATGTATATTTT+TGG + chr2_1:586581-586600 Msa0185960:CDS 25.0%
! TATATACACACTGTGTTTAA+TGG + chr2_1:586647-586666 Msa0185960:intron 25.0%
! TCGTAAGTTTATTACTTCTT+TGG + chr2_1:586685-586704 Msa0185960:intron 25.0%
!!! ATTGAGAAAAGAAAGTGTTT+TGG - chr2_1:586817-586836 None:intergenic 25.0%
!!! ATTTAATTTTAGGGATGTTG+TGG + chr2_1:587056-587075 Msa0185960:intron 25.0%
!! TTTTAGTGATGTACTATGTT+TGG + chr2_1:587088-587107 Msa0185960:CDS 25.0%
! TTGATATTGAAACAATGACT+TGG + chr2_1:587570-587589 Msa0185960:CDS 25.0%
!!! AATAATGATTTTTGGTCACT+AGG - chr2_1:587654-587673 None:intergenic 25.0%
! TTATGACAGTTGAAAAATCA+AGG + chr2_1:587768-587787 Msa0185960:intron 25.0%
!! TTATTTCAGGTATACATCAT+TGG + chr2_1:588209-588228 Msa0185960:intron 25.0%
! ATAGGATAAAGCTAATTTGA+CGG - chr2_1:588398-588417 None:intergenic 25.0%
! AACACTAACAATTCATTCAA+AGG - chr2_1:588539-588558 None:intergenic 25.0%
! AAATGGACATAACAACTTAT+CGG + chr2_1:588580-588599 Msa0185960:CDS 25.0%
!!! TTTGATTTTGAATCCTAGAT+TGG + chr2_1:589487-589506 Msa0185960:intron 25.0%
!! GTATTAGTTCCTTTTATGAT+TGG - chr2_1:589862-589881 None:intergenic 25.0%
! AAATATTGAGGAGGTTAATA+AGG + chr2_1:589983-590002 Msa0185960:three_prime_UTR 25.0%
!! GTAATTGCTCTGTATTTTAT+TGG + chr2_1:590011-590030 Msa0185960:three_prime_UTR 25.0%
!! ATTTTATTGGATGTGTTTGT+AGG + chr2_1:590024-590043 Msa0185960:three_prime_UTR 25.0%
ATCAGTAGACATGAAAAGAT+GGG - chr2_1:586233-586252 None:intergenic 30.0%
TATCAGTAGACATGAAAAGA+TGG - chr2_1:586234-586253 None:intergenic 30.0%
AAAGAAGAAGAGATGAAGAA+AGG - chr2_1:586287-586306 None:intergenic 30.0%
GGATTAAAACCTAAAACCTT+GGG - chr2_1:586527-586546 None:intergenic 30.0%
AGGATTAAAACCTAAAACCT+TGG - chr2_1:586528-586547 None:intergenic 30.0%
!! GTTTTAATCCTTCTCAAAGA+TGG + chr2_1:586537-586556 Msa0185960:CDS 30.0%
TTTAATCCTTCTCAAAGATG+GGG + chr2_1:586539-586558 Msa0185960:CDS 30.0%
TTGTCTTCAACTTCTATCTT+TGG + chr2_1:586784-586803 Msa0185960:intron 30.0%
! GGACACAAAATGATAGTTTT+TGG + chr2_1:587194-587213 Msa0185960:CDS 30.0%
!! CACAAAATGATAGTTTTTGG+TGG + chr2_1:587197-587216 Msa0185960:CDS 30.0%
!!! ATAGTTTTTGGTGGTACAAA+TGG + chr2_1:587206-587225 Msa0185960:CDS 30.0%
!!! TAGTTTTTGGTGGTACAAAT+GGG + chr2_1:587207-587226 Msa0185960:CDS 30.0%
TTGATCTTTACACCAAAGAA+TGG + chr2_1:587258-587277 Msa0185960:CDS 30.0%
TTTAGGCTTAATCCATTCTT+TGG - chr2_1:587273-587292 None:intergenic 30.0%
AATAAGCTTATTGTGTATGG+TGG + chr2_1:587509-587528 Msa0185960:CDS 30.0%
CTAGGAGAATAAAACAACAA+TGG - chr2_1:587956-587975 None:intergenic 30.0%
TAAATCATCACAAGAGTAAG+TGG - chr2_1:588192-588211 None:intergenic 30.0%
CTCTTGTGATGATTTATTTC+AGG + chr2_1:588196-588215 Msa0185960:intron 30.0%
TTGACATACACACTTGTTTA+TGG + chr2_1:588273-588292 Msa0185960:CDS 30.0%
GACACCGATATTGTTATATA+TGG + chr2_1:588359-588378 Msa0185960:CDS 30.0%
ACCGATATTGTTATATATGG+CGG + chr2_1:588362-588381 Msa0185960:CDS 30.0%
TAGGATAAAGCTAATTTGAC+GGG - chr2_1:588397-588416 None:intergenic 30.0%
GATAAGTTGTTATGTCCATT+TGG - chr2_1:588581-588600 None:intergenic 30.0%
AATGGACATAACAACTTATC+GGG + chr2_1:588581-588600 Msa0185960:CDS 30.0%
! TTTTATTGCCTAGTTTGACT+CGG - chr2_1:589171-589190 None:intergenic 30.0%
TCAACAAGATCTTCATGTAA+TGG + chr2_1:589261-589280 Msa0185960:CDS 30.0%
TGAAGATATTGTTCTTGCTT+TGG - chr2_1:589295-589314 None:intergenic 30.0%
CAAGAACAATATCTTCATGT+TGG + chr2_1:589298-589317 Msa0185960:CDS 30.0%
! CATGTTGGTGAAGATAAAAA+AGG + chr2_1:589313-589332 Msa0185960:CDS 30.0%
! ATCAATTTTGCCAAGAACTT+CGG - chr2_1:589515-589534 None:intergenic 30.0%
CATTAGAAAACCTGAATCAA+AGG - chr2_1:589542-589561 None:intergenic 30.0%
AGCAAATTTCAAAAGCTTAC+AGG - chr2_1:589610-589629 None:intergenic 30.0%
GTTACATAAATCGTAGTACT+AGG - chr2_1:589662-589681 None:intergenic 30.0%
CTTGATGGAAACAAACAATT+TGG - chr2_1:589721-589740 None:intergenic 30.0%
ACACAATACTACATAACTCA+TGG - chr2_1:590055-590074 None:intergenic 30.0%
ATATCTAAGTTACCGTTGTA+TGG - chr2_1:590118-590137 None:intergenic 30.0%
TATTCAAAATGTCCTTAGGA+TGG + chr2_1:590204-590223 Msa0185960:three_prime_UTR 30.0%
TAATCAAATGTTCCATCCTA+AGG - chr2_1:590219-590238 None:intergenic 30.0%
GAGACTCCATGAATAATATG+TGG - chr2_1:586463-586482 None:intergenic 35.0%
TATGTGTGCTGAGAGAAATT+AGG - chr2_1:586851-586870 None:intergenic 35.0%
!! TAATTTTAGGGATGTTGTGG+AGG + chr2_1:587059-587078 Msa0185960:intron 35.0%
ATGTTTGGACCTTGACAAAA+TGG + chr2_1:587103-587122 Msa0185960:CDS 35.0%
TGGATTAAGCCTAAATGTGA+AGG + chr2_1:587278-587297 Msa0185960:CDS 35.0%
GGATTAAGCCTAAATGTGAA+GGG + chr2_1:587279-587298 Msa0185960:CDS 35.0%
! GGTGAAAGACTGGTAATTTT+TGG + chr2_1:587347-587366 Msa0185960:CDS 35.0%
!! GAAAGACTGGTAATTTTTGG+TGG + chr2_1:587350-587369 Msa0185960:CDS 35.0%
TAGACCTTAGAACTATGAGA+TGG + chr2_1:587417-587436 Msa0185960:CDS 35.0%
! AACTCCATCTCATAGTTCTA+AGG - chr2_1:587424-587443 None:intergenic 35.0%
! AGTTCTTTTCCTGAGGTTAA+AGG + chr2_1:587440-587459 Msa0185960:CDS 35.0%
AGAGTACAATGACTATCTCT+AGG - chr2_1:587479-587498 None:intergenic 35.0%
! AGTCATTGTACTCTTGCTAT+TGG + chr2_1:587485-587504 Msa0185960:CDS 35.0%
!! GTCATTGTACTCTTGCTATT+GGG + chr2_1:587486-587505 Msa0185960:CDS 35.0%
GGGAATAAGCTTATTGTGTA+TGG + chr2_1:587506-587525 Msa0185960:CDS 35.0%
! TTGAAACAATGACTTGGACT+AGG + chr2_1:587576-587595 Msa0185960:CDS 35.0%
! TGAAACAATGACTTGGACTA+GGG + chr2_1:587577-587596 Msa0185960:CDS 35.0%
AAAAATCAAGGTTCTTCACC+AGG + chr2_1:587780-587799 Msa0185960:CDS 35.0%
!!! AGCCTTTTTTCACGAACAAT+TGG + chr2_1:588124-588143 Msa0185960:intron 35.0%
! TTTCAGGTATACATCATTGG+AGG + chr2_1:588212-588231 Msa0185960:intron 35.0%
TTCAGGTATACATCATTGGA+GGG + chr2_1:588213-588232 Msa0185960:intron 35.0%
AACGTTATTGCAACGACATT+TGG + chr2_1:588246-588265 Msa0185960:CDS 35.0%
ACGTTATTGCAACGACATTT+GGG + chr2_1:588247-588266 Msa0185960:CDS 35.0%
TGGTCTCAACTTGATATACA+TGG + chr2_1:588293-588312 Msa0185960:CDS 35.0%
CCCGCCATATATAACAATAT+CGG - chr2_1:588366-588385 None:intergenic 35.0%
CCGATATTGTTATATATGGC+GGG + chr2_1:588363-588382 Msa0185960:CDS 35.0%
!! TATTGTTATATATGGCGGGT+AGG + chr2_1:588367-588386 Msa0185960:intron 35.0%
AGGATAAAGCTAATTTGACG+GGG - chr2_1:588396-588415 None:intergenic 35.0%
AAAAAGAGCTGCTAAGGAAT+AGG - chr2_1:588416-588435 None:intergenic 35.0%
! TAGTGGTAATGTTAGTTTCC+AGG + chr2_1:588458-588477 Msa0185960:intron 35.0%
AGGCTATCATAATGAAATCC+TGG - chr2_1:588479-588498 None:intergenic 35.0%
ATGGACATAACAACTTATCG+GGG + chr2_1:588582-588601 Msa0185960:CDS 35.0%
CTACCTGAATCAAATTGACA+CGG - chr2_1:588908-588927 None:intergenic 35.0%
TGTTCTTGATCAGATTGAGA+TGG - chr2_1:589097-589116 None:intergenic 35.0%
GAAGTTCTTGGCAAAATTGA+TGG + chr2_1:589514-589533 Msa0185960:CDS 35.0%
TAATGACTGCAGTAGTGAAT+GGG + chr2_1:589557-589576 Msa0185960:CDS 35.0%
! ACATGATTTGAGTTCATGAG+AGG - chr2_1:589748-589767 None:intergenic 35.0%
TCTCATGAACTCAAATCATG+TGG + chr2_1:589747-589766 Msa0185960:CDS 35.0%
CAAATCATGTGGATAATTCG+AGG + chr2_1:589758-589777 Msa0185960:CDS 35.0%
! GTTCCTTTTATGATTGGAGA+CGG - chr2_1:589856-589875 None:intergenic 35.0%
ACTAATACAGTGTCTTTACC+CGG + chr2_1:589874-589893 Msa0185960:CDS 35.0%
! ACTCCTGATTTTATGTTCTC+CGG - chr2_1:589896-589915 None:intergenic 35.0%
GGAAATAATGCTTGATCCTT+AGG + chr2_1:589958-589977 Msa0185960:exon 35.0%
GATCCTTAGGTGAAATATTG+AGG + chr2_1:589971-589990 Msa0185960:exon 35.0%
CAAGCTCTTCATCATTAACA+TGG - chr2_1:590170-590189 None:intergenic 35.0%
CATGTTAATGATGAAGAGCT+TGG + chr2_1:590168-590187 Msa0185960:three_prime_UTR 35.0%
CTCCTAAGAAGAAAGCAATG+TGG + chr2_1:586495-586514 Msa0185960:CDS 40.0%
AGCCACATTGCTTTCTTCTT+AGG - chr2_1:586500-586519 None:intergenic 40.0%
TGGACCTTGACAAAATGGAA+TGG + chr2_1:587108-587127 Msa0185960:CDS 40.0%
! TGCTCCATTCCATTTTGTCA+AGG - chr2_1:587115-587134 None:intergenic 40.0%
TGACAAAATGGAATGGAGCA+AGG + chr2_1:587115-587134 Msa0185960:CDS 40.0%
!!! CTGGTAATTTTTGGTGGTAG+TGG + chr2_1:587356-587375 Msa0185960:CDS 40.0%
!!! ATTTTTGGTGGTAGTGGAGA+AGG + chr2_1:587362-587381 Msa0185960:CDS 40.0%
! CAATGACTATCTCTAGGAAC+AGG - chr2_1:587473-587492 None:intergenic 40.0%
! ATTGTGTATGGTGGAGATAG+TGG + chr2_1:587518-587537 Msa0185960:CDS 40.0%
! GATAGTGGTGATCAGTATCA+TGG + chr2_1:587533-587552 Msa0185960:CDS 40.0%
AGCTGTGAATGTTGGAACTA+AGG + chr2_1:587821-587840 Msa0185960:CDS 40.0%
GTTCGTGAAAAAAGGCTAGA+CGG - chr2_1:588121-588140 None:intergenic 40.0%
GGCCAATTGTTCGTGAAAAA+AGG - chr2_1:588129-588148 None:intergenic 40.0%
TCAGGTATACATCATTGGAG+GGG + chr2_1:588214-588233 Msa0185960:intron 40.0%
CGATACAAAAAGAGCTGCTA+AGG - chr2_1:588422-588441 None:intergenic 40.0%
! AGCAGCTCTTTTTGTATCGA+CGG + chr2_1:588423-588442 Msa0185960:intron 40.0%
GACGGATGTTAGTAAGTTAG+TGG + chr2_1:588441-588460 Msa0185960:intron 40.0%
GCAAAGTCTTCGGAAGTTAT+TGG + chr2_1:588606-588625 Msa0185960:CDS 40.0%
TCAAGAAAAGAAGACCATCC+TGG + chr2_1:588631-588650 Msa0185960:CDS 40.0%
GACCAGAAAATTTCGCGTAT+CGG + chr2_1:588824-588843 Msa0185960:intron 40.0%
ACCAGAAAATTTCGCGTATC+GGG + chr2_1:588825-588844 Msa0185960:intron 40.0%
ATGTGGAAGTCTTGGGAAAA+TGG + chr2_1:588852-588871 Msa0185960:CDS 40.0%
TGTGGAAGTCTTGGGAAAAT+GGG + chr2_1:588853-588872 Msa0185960:CDS 40.0%
CGTGTCAATTTGATTCAGGT+AGG + chr2_1:588906-588925 Msa0185960:intron 40.0%
!!! GAAGCATTTGGTTTTTGACC+TGG - chr2_1:589124-589143 None:intergenic 40.0%
AAAACCAAATGCTTCTGCCA+TGG + chr2_1:589129-589148 Msa0185960:CDS 40.0%
!! AAATCCATGGCAGAAGCATT+TGG - chr2_1:589136-589155 None:intergenic 40.0%
!! TTGTCAAGTTGACAAGTGCT+TGG - chr2_1:589196-589215 None:intergenic 40.0%
AAGAACTTCGGTACCAATCT+AGG - chr2_1:589503-589522 None:intergenic 40.0%
! TAGATTGGTACCGAAGTTCT+TGG + chr2_1:589502-589521 Msa0185960:intron 40.0%
ATTGATGGAGCCTTTGATTC+AGG + chr2_1:589529-589548 Msa0185960:CDS 40.0%
CTAATGACTGCAGTAGTGAA+TGG + chr2_1:589556-589575 Msa0185960:CDS 40.0%
TGAATAATCTATCAGGGAGC+AGG + chr2_1:589685-589704 Msa0185960:intron 40.0%
! AGCAAGTAGTGGTTGCTTTA+GGG - chr2_1:589830-589849 None:intergenic 40.0%
TACCCGGAGAACATAAAATC+AGG + chr2_1:589890-589909 Msa0185960:CDS 40.0%
! CTCCTGATTTTATGTTCTCC+GGG - chr2_1:589895-589914 None:intergenic 40.0%
! AAAATCAGGAGTGACTTGCA+AGG + chr2_1:589904-589923 Msa0185960:CDS 40.0%
!! CAAGGTTTGGCTTTAACACT+AGG + chr2_1:589922-589941 Msa0185960:CDS 40.0%
!! GTTTGGCTTTAACACTAGGA+GGG + chr2_1:589926-589945 Msa0185960:CDS 40.0%
CAAGCATTATTTCCACTTGC+AGG - chr2_1:589952-589971 None:intergenic 40.0%
CCTCCTCAATATTTCACCTA+AGG - chr2_1:589977-589996 None:intergenic 40.0%
CCTTAGGTGAAATATTGAGG+AGG + chr2_1:589974-589993 Msa0185960:three_prime_UTR 40.0%
CTAAGTTACCGTTGTATGGT+CGG - chr2_1:590114-590133 None:intergenic 40.0%
CTCTGCTGTGATTGTGAATG+TGG - chr2_1:586403-586422 None:intergenic 45.0%
! TGGCTGTATCCCAAGGTTTT+AGG + chr2_1:586515-586534 Msa0185960:CDS 45.0%
TGGAGCAAGGTTACTACAAC+AGG + chr2_1:587128-587147 Msa0185960:CDS 45.0%
ACTACAACAGGTGAAAAGCC+AGG + chr2_1:587140-587159 Msa0185960:CDS 45.0%
ACAGCACTATGGCTATCTCT+AGG - chr2_1:587167-587186 None:intergenic 45.0%
! AGCCATAGTGCTGTTCTTGT+AGG + chr2_1:587173-587192 Msa0185960:CDS 45.0%
GTCCTACAAGAACAGCACTA+TGG - chr2_1:587178-587197 None:intergenic 45.0%
GGTGGTATCCCTTCACATTT+AGG - chr2_1:587290-587309 None:intergenic 45.0%
AGTCACACTGCTACTCTTGT+TGG + chr2_1:587323-587342 Msa0185960:CDS 45.0%
!! TCTTGTTGGTGGTGAAAGAC+TGG + chr2_1:587337-587356 Msa0185960:CDS 45.0%
! GAGATGGAGTTCTTTTCCTG+AGG + chr2_1:587433-587452 Msa0185960:CDS 45.0%
! CAATGACTTGGACTAGGGTA+TGG + chr2_1:587582-587601 Msa0185960:intron 45.0%
GTATACATCATTGGAGGGGT+CGG + chr2_1:588218-588237 Msa0185960:CDS 45.0%
ATACATGGTCAACAGCCACA+AGG + chr2_1:588308-588327 Msa0185960:CDS 45.0%
GTATGAGAAAACCGTCCTTG+TGG - chr2_1:588326-588345 None:intergenic 45.0%
TTATCGGGGTGCAAAGTCTT+CGG + chr2_1:588596-588615 Msa0185960:CDS 45.0%
GAAAAGAAGACCATCCTGGA+TGG + chr2_1:588635-588654 Msa0185960:CDS 45.0%
TGGCGGAGTAGATACAAACA+CGG + chr2_1:588655-588674 Msa0185960:CDS 45.0%
! TCCCGATACGCGAAATTTTC+TGG - chr2_1:588829-588848 None:intergenic 45.0%
GGGAATGATGTGGAAGTCTT+GGG + chr2_1:588845-588864 Msa0185960:CDS 45.0%
GTGGAAGTCTTGGGAAAATG+GGG + chr2_1:588854-588873 Msa0185960:CDS 45.0%
GTCTTGGGAAAATGGGGTTA+TGG + chr2_1:588860-588879 Msa0185960:CDS 45.0%
CGACCGTGTCAATTTGATTC+AGG + chr2_1:588902-588921 Msa0185960:CDS 45.0%
CAGGAACTTCACAACAGAAG+AGG + chr2_1:588990-589009 Msa0185960:intron 45.0%
GAACTTCACAACAGAAGAGG+AGG + chr2_1:588993-589012 Msa0185960:CDS 45.0%
! AATTGCTGCCACAAAGGTGT+GGG + chr2_1:589018-589037 Msa0185960:CDS 45.0%
TTCAACATCCCACACCTTTG+TGG - chr2_1:589029-589048 None:intergenic 45.0%
TGGAGATGAATGCTGAGACA+GGG - chr2_1:589077-589096 None:intergenic 45.0%
ATGGAGATGAATGCTGAGAC+AGG - chr2_1:589078-589097 None:intergenic 45.0%
TCTGATCAAGAACAGACTCC+AGG + chr2_1:589103-589122 Msa0185960:CDS 45.0%
GCGATATCGCTGAAAATCCA+TGG - chr2_1:589149-589168 None:intergenic 45.0%
! GTGAATGGGAGACTTTTCAG+AGG + chr2_1:589571-589590 Msa0185960:CDS 45.0%
!! TTCATGAGAGGCTGACTTGA+TGG - chr2_1:589736-589755 None:intergenic 45.0%
! GAGCAAGTAGTGGTTGCTTT+AGG - chr2_1:589831-589850 None:intergenic 45.0%
CGACCGTCTCCAATCATAAA+AGG + chr2_1:589850-589869 Msa0185960:CDS 45.0%
!! GGTTTGGCTTTAACACTAGG+AGG + chr2_1:589925-589944 Msa0185960:CDS 45.0%
!!! ATTTGATTTAATTTAATTTT+AGG + chr2_1:587046-587065 Msa0185960:intron 5.0%
!!! TTTGATTTAATTTAATTTTA+GGG + chr2_1:587047-587066 Msa0185960:intron 5.0%
!! ATTAAAATATATATGAATTT+AGG - chr2_1:587930-587949 None:intergenic 5.0%
AGCAATGTGGCTGTATCCCA+AGG + chr2_1:586508-586527 Msa0185960:CDS 50.0%
! GAGTGACCCCATCTTTGAGA+AGG - chr2_1:586548-586567 None:intergenic 50.0%
!! CTATGGCTATCTCTAGGACC+TGG - chr2_1:587161-587180 None:intergenic 50.0%
AAGGGATACCACCTTCACCA+AGG + chr2_1:587297-587316 Msa0185960:CDS 50.0%
AGGGATACCACCTTCACCAA+GGG + chr2_1:587298-587317 Msa0185960:CDS 50.0%
TGACTTTCCCTTGGTGAAGG+TGG - chr2_1:587308-587327 None:intergenic 50.0%
GTGTGACTTTCCCTTGGTGA+AGG - chr2_1:587311-587330 None:intergenic 50.0%
GTAGCAGTGTGACTTTCCCT+TGG - chr2_1:587317-587336 None:intergenic 50.0%
CACACTGCTACTCTTGTTGG+TGG + chr2_1:587326-587345 Msa0185960:CDS 50.0%
GGCACATCACCTTTAACCTC+AGG - chr2_1:587452-587471 None:intergenic 50.0%
AAGGTTCTTCACCAGGAGTC+AGG + chr2_1:587787-587806 Msa0185960:CDS 50.0%
AGGTTCTTCACCAGGAGTCA+GGG + chr2_1:587788-587807 Msa0185960:CDS 50.0%
GGTCATGCAGCTGTGAATGT+TGG + chr2_1:587813-587832 Msa0185960:CDS 50.0%
ATGGTCAACAGCCACAAGGA+CGG + chr2_1:588312-588331 Msa0185960:CDS 50.0%
! ATCGGTGTCTGCAACAACAG+CGG - chr2_1:588348-588367 None:intergenic 50.0%
ACCTGCTACAAGTCAGAGAC+AGG - chr2_1:588499-588518 None:intergenic 50.0%
! TCTCTGACTTGTAGCAGGTG+TGG + chr2_1:588500-588519 Msa0185960:intron 50.0%
! CTCTGACTTGTAGCAGGTGT+GGG + chr2_1:588501-588520 Msa0185960:intron 50.0%
! TCTGACTTGTAGCAGGTGTG+GGG + chr2_1:588502-588521 Msa0185960:intron 50.0%
! CTTGCAGCAGAGCATCCAAA+TGG + chr2_1:588563-588582 Msa0185960:CDS 50.0%
AAGAAGACCATCCTGGATGG+CGG + chr2_1:588638-588657 Msa0185960:CDS 50.0%
TTGTATCTACTCCGCCATCC+AGG - chr2_1:588652-588671 None:intergenic 50.0%
TTCGCGTATCGGGAATGATG+TGG + chr2_1:588835-588854 Msa0185960:CDS 50.0%
CGGGAATGATGTGGAAGTCT+TGG + chr2_1:588844-588863 Msa0185960:CDS 50.0%
GAGGAGAATTGCTGCCACAA+AGG + chr2_1:589012-589031 Msa0185960:CDS 50.0%
GAATTGCTGCCACAAAGGTG+TGG + chr2_1:589017-589036 Msa0185960:CDS 50.0%
CGATATCGCCGAGTCAAACT+AGG + chr2_1:589160-589179 Msa0185960:CDS 50.0%
!! CAGGCTTCGGTTCTAAAGCT+TGG - chr2_1:589348-589367 None:intergenic 50.0%
ACGCGGATACACTCAACACT+TGG + chr2_1:589372-589391 Msa0185960:CDS 50.0%
!! GGTTGCTTTAGGGCATAGCA+CGG - chr2_1:589820-589839 None:intergenic 50.0%
! CAGGAGTGACTTGCAAGGTT+TGG + chr2_1:589909-589928 Msa0185960:CDS 50.0%
CGTATCTGCCGACCATACAA+CGG + chr2_1:590103-590122 Msa0185960:three_prime_UTR 50.0%
GGTCACTCTGCTTGCTTCTC+TGG + chr2_1:586560-586579 Msa0185960:CDS 55.0%
! GCCTGTCTCTGACTTGTAGC+AGG + chr2_1:588495-588514 Msa0185960:intron 55.0%
ATCTACTCCGCCATCCAGGA+TGG - chr2_1:588648-588667 None:intergenic 55.0%
!! GCTTTAGAACCGAAGCCTGC+CGG + chr2_1:589349-589368 Msa0185960:CDS 55.0%
GTGTTGAGTGTATCCGCGTC+CGG - chr2_1:589371-589390 None:intergenic 55.0%
TCTTCACCAGGAGTCAGGGC+AGG + chr2_1:587792-587811 Msa0185960:CDS 60.0%
GGAGACGGTCGAGCAAGTAG+TGG - chr2_1:589841-589860 None:intergenic 60.0%
ACACTAGGAGGGCCTGCAAG+TGG + chr2_1:589937-589956 Msa0185960:CDS 60.0%
GCATGACCTGCCCTGACTCC+TGG - chr2_1:587801-587820 None:intergenic 65.0%
TATCCGCGTCCGGCAGGCTT+CGG - chr2_1:589361-589380 None:intergenic 65.0%
TGAGTGTATCCGCGTCCGGC+AGG - chr2_1:589367-589386 None:intergenic 65.0%
GAACCGAAGCCTGCCGGACG+CGG + chr2_1:589355-589374 Msa0185960:CDS 70.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 586213 590264 586213 ID=Msa0185960;Name=Msa0185960
chr2_1 mRNA 586213 590264 586213 ID=Msa0185960-mRNA-1;Parent=Msa0185960;Name=Msa0185960-mRNA-1;_AED=0.10;_eAED=0.10;_QI=260|0.87|0.88|1|1|1|9|292|649
chr2_1 exon 586213 586604 586213 ID=Msa0185960-mRNA-1:exon:104;Parent=Msa0185960-mRNA-1
chr2_1 exon 587068 587598 587068 ID=Msa0185960-mRNA-1:exon:105;Parent=Msa0185960-mRNA-1
chr2_1 exon 587777 587863 587777 ID=Msa0185960-mRNA-1:exon:106;Parent=Msa0185960-mRNA-1
chr2_1 exon 588218 588384 588218 ID=Msa0185960-mRNA-1:exon:107;Parent=Msa0185960-mRNA-1
chr2_1 exon 588517 588676 588517 ID=Msa0185960-mRNA-1:exon:108;Parent=Msa0185960-mRNA-1
chr2_1 exon 588830 588923 588830 ID=Msa0185960-mRNA-1:exon:109;Parent=Msa0185960-mRNA-1
chr2_1 exon 588993 589393 588993 ID=Msa0185960-mRNA-1:exon:110;Parent=Msa0185960-mRNA-1
chr2_1 exon 589505 589609 589505 ID=Msa0185960-mRNA-1:exon:111;Parent=Msa0185960-mRNA-1
chr2_1 exon 589700 590264 589700 ID=Msa0185960-mRNA-1:exon:112;Parent=Msa0185960-mRNA-1
chr2_1 five_prime_UTR 586213 586472 586213 ID=Msa0185960-mRNA-1:five_prime_utr;Parent=Msa0185960-mRNA-1
chr2_1 CDS 586473 586604 586473 ID=Msa0185960-mRNA-1:cds;Parent=Msa0185960-mRNA-1
chr2_1 CDS 587068 587598 587068 ID=Msa0185960-mRNA-1:cds;Parent=Msa0185960-mRNA-1
chr2_1 CDS 587777 587863 587777 ID=Msa0185960-mRNA-1:cds;Parent=Msa0185960-mRNA-1
chr2_1 CDS 588218 588384 588218 ID=Msa0185960-mRNA-1:cds;Parent=Msa0185960-mRNA-1
chr2_1 CDS 588517 588676 588517 ID=Msa0185960-mRNA-1:cds;Parent=Msa0185960-mRNA-1
chr2_1 CDS 588830 588923 588830 ID=Msa0185960-mRNA-1:cds;Parent=Msa0185960-mRNA-1
chr2_1 CDS 588993 589393 588993 ID=Msa0185960-mRNA-1:cds;Parent=Msa0185960-mRNA-1
chr2_1 CDS 589505 589609 589505 ID=Msa0185960-mRNA-1:cds;Parent=Msa0185960-mRNA-1
chr2_1 CDS 589700 589972 589700 ID=Msa0185960-mRNA-1:cds;Parent=Msa0185960-mRNA-1
chr2_1 three_prime_UTR 589973 590264 589973 ID=Msa0185960-mRNA-1:three_prime_utr;Parent=Msa0185960-mRNA-1
Gene Sequence

>Msa0185960

ATGGAGTCTCTTCTTGCTGAAACTCCTAAGAAGAAAGCAATGTGGCTGTATCCCAAGGTTTTAGGTTTTAATCCTTCTCAAAGATGGGGTCACTCTGCTTGCTTCTCTGGAAGACTTATGTATATTTTTGGGGGATGTTGTGGAGGATTACATTTTAGTGATGTACTATGTTTGGACCTTGACAAAATGGAATGGAGCAAGGTTACTACAACAGGTGAAAAGCCAGGTCCTAGAGATAGCCATAGTGCTGTTCTTGTAGGACACAAAATGATAGTTTTTGGTGGTACAAATGGGTTCAAGAAAGTGAATGAAACTCATATACTTGATCTTTACACCAAAGAATGGATTAAGCCTAAATGTGAAGGGATACCACCTTCACCAAGGGAAAGTCACACTGCTACTCTTGTTGGTGGTGAAAGACTGGTAATTTTTGGTGGTAGTGGAGAAGGTGATGCAAATTATTTAAATGATTTGCATATTTTAGACCTTAGAACTATGAGATGGAGTTCTTTTCCTGAGGTTAAAGGTGATGTGCCTGTTCCTAGAGATAGTCATTGTACTCTTGCTATTGGGAATAAGCTTATTGTGTATGGTGGAGATAGTGGTGATCAGTATCATGGTGATGTAAGTTTGCTTGATATTGAAACAATGACTTGGACTAGGTTGAAAAATCAAGGTTCTTCACCAGGAGTCAGGGCAGGTCATGCAGCTGTGAATGTTGGAACTAAGGCAAGTGATTTTACTCATTCAGTATACATCATTGGAGGGGTCGGAGATAAACGTTATTGCAACGACATTTGGGTGTTTGACATACACACTTGTTTATGGTCTCAACTTGATATACATGGTCAACAGCCACAAGGACGGTTTTCTCATACCGCTGTTGTTGCAGACACCGATATTGTTATATATGGCGGGTGTGGGGAAGATGAACGTCCTTTGAATGAATTGTTAGTGTTGCAGCTTGCAGCAGAGCATCCAAATGGACATAACAACTTATCGGGGTGCAAAGTCTTCGGAAGTTATTGGAATCAAGAAAAGAAGACCATCCTGGATGGCGGAGTAGATACAAACACGAAAATTTCGCGTATCGGGAATGATGTGGAAGTCTTGGGAAAATGGGGTTATGGAGTTGCATCAGAGAAAGCACGACCGTGTCAATTTGATTCAGGAACTTCACAACAGAAGAGGAGGAGAATTGCTGCCACAAAGGTGTGGGATGTTGAATCAGAACAAGAAGAACATTCTCTTTCCCTGTCTCAGCATTCATCTCCATCTCAATCTGATCAAGAACAGACTCCAGGTCAAAAACCAAATGCTTCTGCCATGGATTTTCAGCGATATCGCCGAGTCAAACTAGGCAATAAAACTCCAAGCACTTGTCAACTTGACAATTTCTCAAGTAACAAGAGATTTCTGAAGAATTTTACACAAATAAGTCAACAAGATCTTCATGTAATGGATCATCAACCAAAGCAAGAACAATATCTTCATGTTGGTGAAGATAAAAAAGGAGCTCGACTCCAAGCTTTAGAACCGAAGCCTGCCGGACGCGGATACACTCAACACTTGATTGGTACCGAAGTTCTTGGCAAAATTGATGGAGCCTTTGATTCAGGTTTTCTAATGACTGCAGTAGTGAATGGGAGACTTTTCAGAGGAGTTTTATTTGCACCTGGAGCAGGAGTAGCTGAGCCAAATTGTTTGTTTCCATCAAGTCAGCCTCTCATGAACTCAAATCATGTGGATAATTCGAGGAATTCAGAGAAAGTAACTAACTGTTCGCGTTTAGAGCCGTGCTATGCCCTAAAGCAACCACTACTTGCTCGACCGTCTCCAATCATAAAAGGAACTAATACAGTGTCTTTACCCGGAGAACATAAAATCAGGAGTGACTTGCAAGGTTTGGCTTTAACACTAGGAGGGCCTGCAAGTGGAAATAATGCTTGA
Protein sequence

>Msa0185960

MESLLAETPKKKAMWLYPKVLGFNPSQRWGHSACFSGRLMYIFGGCCGGLHFSDVLCLDLDKMEWSKVTTTGEKPGPRDSHSAVLVGHKMIVFGGTNGFKKVNETHILDLYTKEWIKPKCEGIPPSPRESHTATLVGGERLVIFGGSGEGDANYLNDLHILDLRTMRWSSFPEVKGDVPVPRDSHCTLAIGNKLIVYGGDSGDQYHGDVSLLDIETMTWTRLKNQGSSPGVRAGHAAVNVGTKASDFTHSVYIIGGVGDKRYCNDIWVFDIHTCLWSQLDIHGQQPQGRFSHTAVVADTDIVIYGGCGEDERPLNELLVLQLAAEHPNGHNNLSGCKVFGSYWNQEKKTILDGGVDTNTKISRIGNDVEVLGKWGYGVASEKARPCQFDSGTSQQKRRRIAATKVWDVESEQEEHSLSLSQHSSPSQSDQEQTPGQKPNASAMDFQRYRRVKLGNKTPSTCQLDNFSSNKRFLKNFTQISQQDLHVMDHQPKQEQYLHVGEDKKGARLQALEPKPAGRGYTQHLIGTEVLGKIDGAFDSGFLMTAVVNGRLFRGVLFAPGAGVAEPNCLFPSSQPLMNSNHVDNSRNSEKVTNCSRLEPCYALKQPLLARPSPIIKGTNTVSLPGEHKIRSDLQGLALTLGGPASGNNA*