AlfalfaGEDB | Msa0369400

M. sativa cultivar zhongmu-4 / Msa0369400

KEGG: K11254
GO: GO:0000228 GO:0000785 GO:0000786 GO:0000788 GO:0000790 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006323 GO:0006325 GO:0006333 GO:0006334 GO:0006996 GO:0008150 GO:0009987 GO:0016043 GO:0022607 GO:0031497 GO:0031974 GO:0031981 GO:0032991 GO:0032993 GO:0034622 GO:0034728 GO:0042393 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044815 GO:0051276 GO:0065003 GO:0065004 GO:0070013 GO:0071103 GO:0071824 GO:0071840 GO:0097159 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0369400	RCV35100.1	100.000	103	0	0	1	103	130	232	3.26e-67	211

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0369400	sp\|P0CG89\|H4_SOYBN	100.000	103	0	0	1	103	1	103	3.06e-67	199

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0369400	A0A7C8Z1D6	100.000	103	0	0	1	103	1	103	1.17e-64	199

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0005890	Msa0369400	0.814352	1.609381e-51	-8.615850e-47
Msa0028420	Msa0369400	0.803130	4.036813e-49	-8.615850e-47
Msa0054690	Msa0369400	0.822234	2.642265e-53	-8.615850e-47
Msa0223080	Msa0369400	0.860092	2.628705e-63	-8.615850e-47
Msa0369400	Msa0403970	0.810677	1.023516e-50	-8.615850e-47
Msa0369400	Msa0405680	0.809046	2.296304e-50	-8.615850e-47
Msa0369400	Msa0580370	0.836197	1.077352e-56	-8.615850e-47
Msa0369400	Msa0697160	0.806316	8.727820e-50	-8.615850e-47
Msa0369400	Msa0795870	0.828099	1.085388e-54	-8.615850e-47
Msa0369400	Msa0824450	0.814240	1.704351e-51	-8.615850e-47
Msa0369400	Msa0833650	0.830993	2.146721e-55	-8.615850e-47
Msa0369400	Msa0835960	0.815500	8.954695e-52	-8.615850e-47
Msa0369400	Msa1014370	0.816218	6.194206e-52	-8.615850e-47
Msa0369400	Msa1078170	0.811183	7.954135e-51	-8.615850e-47
Msa0369400	Msa1085260	0.803285	3.750591e-49	-8.615850e-47
Msa0369400	Msa1103990	0.832809	7.648305e-56	-8.615850e-47
Msa0369400	Msa1132840	0.837372	5.403511e-57	-8.615850e-47
Msa0369400	Msa1200890	0.818662	1.744161e-52	-8.615850e-47
Msa0369400	Msa1221060	0.806044	9.959994e-50	-8.615850e-47
Msa0369400	Msa1251160	0.812617	3.874501e-51	-8.615850e-47
Msa0369400	Msa1359690	0.812321	4.496586e-51	-8.615850e-47
Msa0369400	Msa1361410	0.802793	4.738802e-49	-8.615850e-47
Msa0369400	Msa1373100	0.825647	4.181366e-54	-8.615850e-47
Msa0369400	Msa1422950	0.825365	4.877300e-54	-8.615850e-47
Msa0369400	Msa1440880	0.827209	1.775444e-54	-8.615850e-47
Msa0369400	Msa1446820	0.854573	1.124900e-61	-8.615850e-47
Msa0369400	Msa1451160	0.823896	1.081895e-53	-8.615850e-47
Msa0302160	Msa0369400	0.853173	2.845948e-61	-8.615850e-47
Msa0330380	Msa0369400	0.811724	6.066443e-51	-8.615850e-47
Msa0339490	Msa0369400	0.852267	5.162487e-61	-8.615850e-47
Msa0339500	Msa0369400	0.847354	1.219196e-59	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	q. start	q. end	s. start	s. end	e-value	bit score
Msa0369400	MtrunA17_Chr4g0072411	100.000	103	1	103	59	161	5.39e-69	203
Msa0369400	MtrunA17_Chr5g0425951	100.000	103	1	103	1	103	2.26e-68	199
Msa0369400	MtrunA17_Chr4g0030431	100.000	103	1	103	1	103	2.26e-68	199
Msa0369400	MtrunA17_Chr3g0100121	100.000	103	1	103	1	103	2.26e-68	199
Msa0369400	MtrunA17_Chr3g0113681	100.000	103	1	103	1	103	2.26e-68	199
Msa0369400	MtrunA17_Chr2g0327641	100.000	103	1	103	1	103	2.26e-68	199
Msa0369400	MtrunA17_Chr3g0103351	100.000	103	1	103	1	103	2.26e-68	199
Msa0369400	MtrunA17_Chr8g0352791	100.000	103	1	103	1	103	2.26e-68	199
Msa0369400	MtrunA17_Chr3g0103821	100.000	103	1	103	1	103	2.26e-68	199
Msa0369400	MtrunA17_Chr3g0104031	100.000	103	1	103	1	103	2.26e-68	199
Msa0369400	MtrunA17_Chr7g0225731	100.000	103	1	103	1	103	2.26e-68	199
Msa0369400	MtrunA17_Chr3g0103801	100.000	103	1	103	1	103	2.26e-68	199
Msa0369400	MtrunA17_Chr3g0103881	100.000	103	1	103	1	103	2.26e-68	199
Msa0369400	MtrunA17_Chr7g0262791	100.000	103	1	103	1	103	2.26e-68	199

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0369400	AT3G53730.1	100.000	103	0	1	103	1	103	3.11e-68	199
Msa0369400	AT1G07820.2	100.000	103	0	1	103	1	103	3.11e-68	199
Msa0369400	AT1G07820.1	100.000	103	0	1	103	1	103	3.11e-68	199
Msa0369400	AT5G59690.1	100.000	103	0	1	103	1	103	3.11e-68	199
Msa0369400	AT3G46320.1	100.000	103	0	1	103	1	103	3.11e-68	199
Msa0369400	AT2G28740.1	100.000	103	0	1	103	1	103	3.11e-68	199
Msa0369400	AT1G07660.1	100.000	103	0	1	103	1	103	3.11e-68	199
Msa0369400	AT3G45930.1	100.000	103	0	1	103	1	103	3.11e-68	199
Msa0369400	AT5G59970.1	100.000	103	0	1	103	1	103	3.11e-68	199
Msa0369400	AT5G59970.2	100.000	103	0	1	103	1	103	3.11e-68	199
Msa0369400	AT1G07660.2	83.495	103	1	1	103	1	86	4.52e-51	155

Find 57 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGAGAAATTTGAAGAAATT+AGG	0.175437	3_1:+66596932	None:intergenic
ATGTCATGTACTTAACAATT+TGG	0.231439	3_1:+66596273	None:intergenic
ATTATTGTTTAGCTAGTAAT+TGG	0.260150	3_1:-66596513	Msa0369400:three_prime_UTR
TCTAATGAAGTAATCGTTTC+CGG	0.305170	3_1:-66596414	Msa0369400:three_prime_UTR
TGAGAAATTTGAAGAAATTA+GGG	0.309015	3_1:+66596933	None:intergenic
AAGCACCTTCCTATGCCTTT+TGG	0.317118	3_1:+66596828	None:intergenic
TTTCATCTCTTGAAAATGTC+TGG	0.348014	3_1:-66596889	Msa0369400:exon
AGGCAGGGAAGAACCCTCTA+TGG	0.361242	3_1:-66596598	Msa0369400:CDS
TAAGCCTGCGATTCGTCGTT+TGG	0.371129	3_1:-66596782	Msa0369400:CDS
AGGTGTCTTGAAGATCTTCT+TGG	0.406404	3_1:-66596707	Msa0369400:CDS
GGAAGAACCCTCTATGGGTT+TGG	0.426577	3_1:-66596592	Msa0369400:CDS
GGAGGTAAGGGACTAGGAAA+GGG	0.443358	3_1:-66596856	Msa0369400:CDS
GTGAGAGCGGGATGAATAAG+AGG	0.443580	3_1:+66597021	None:intergenic
ATCAACCTCCAAACCCATAG+AGG	0.443645	3_1:+66596584	None:intergenic
GGGAGGTAAGGGACTAGGAA+AGG	0.449480	3_1:-66596857	Msa0369400:CDS
TGGAGTGGGATAAGAGAGAA+GGG	0.453257	3_1:+66596969	None:intergenic
TGGTCGTGGTAAGGGAGGTA+AGG	0.462120	3_1:-66596869	Msa0369400:CDS
AATTTGAAGAAATTAGGGTT+TGG	0.473689	3_1:+66596938	None:intergenic
GAAAATGTCTGGTCGTGGTA+AGG	0.478730	3_1:-66596878	Msa0369400:exon
TGTCTATGCTTTGAAGAGGC+AGG	0.485721	3_1:-66596614	Msa0369400:CDS
AGAACCCTCTATGGGTTTGG+AGG	0.501382	3_1:-66596589	Msa0369400:CDS
TTGGAGTGGGATAAGAGAGA+AGG	0.510728	3_1:+66596968	None:intergenic
GGTGCTTCGCGATAACATCC+AGG	0.510741	3_1:-66596812	Msa0369400:CDS
GAAGATCTTCAAGACACCTC+TGG	0.510902	3_1:+66596711	None:intergenic
CTCGCCAAACGACGAATCGC+AGG	0.516571	3_1:+66596778	None:intergenic
ATTCGTCGTTTGGCGAGAAG+AGG	0.517863	3_1:-66596772	Msa0369400:CDS
GGTAAGGGAGGTAAGGGACT+AGG	0.518213	3_1:-66596862	Msa0369400:CDS
TTCGTCGTTTGGCGAGAAGA+GGG	0.520355	3_1:-66596771	Msa0369400:CDS
AAAATGTCTGGTCGTGGTAA+GGG	0.526072	3_1:-66596877	Msa0369400:CDS
TGCAATTTAGTATAATCATA+CGG	0.528562	3_1:-66596351	Msa0369400:three_prime_UTR
GTCTATGCTTTGAAGAGGCA+GGG	0.532756	3_1:-66596613	Msa0369400:CDS
GGGATCTGAGTATTTATGAA+TGG	0.541111	3_1:+66596990	None:intergenic
CGTCGTTTGGCGAGAAGAGG+GGG	0.550836	3_1:-66596769	Msa0369400:CDS
CTCTTGAAAATGTCTGGTCG+TGG	0.562247	3_1:-66596883	Msa0369400:exon
GGTAAGGGACTAGGAAAGGG+AGG	0.563878	3_1:-66596853	Msa0369400:CDS
TAGGAAAGGGAGGTGCCAAA+AGG	0.574680	3_1:-66596843	Msa0369400:CDS
TAGCTAGTAATTGGAACTCG+TGG	0.581433	3_1:-66596504	Msa0369400:three_prime_UTR
AGGGAGGTGCCAAAAGGCAT+AGG	0.583251	3_1:-66596837	Msa0369400:CDS
TCGTCGTTTGGCGAGAAGAG+GGG	0.586238	3_1:-66596770	Msa0369400:CDS
TTACATACACTGAGCATGCA+AGG	0.586915	3_1:-66596663	Msa0369400:CDS
TCGCAGGCTTAGTGATACCC+TGG	0.594313	3_1:+66596794	None:intergenic
GGCAGGGAAGAACCCTCTAT+GGG	0.597151	3_1:-66596597	Msa0369400:CDS
GAGGAAGACTGTGACTGCCA+TGG	0.604963	3_1:-66596641	Msa0369400:CDS
GGTCGTGGTAAGGGAGGTAA+GGG	0.613033	3_1:-66596868	Msa0369400:CDS
AAACTAGGAGCATAGTTCAC+CGG	0.617092	3_1:+66596395	None:intergenic
AATGGTAGTGAGCGTGAGAG+CGG	0.620658	3_1:+66597008	None:intergenic
ATGGTAGTGAGCGTGAGAGC+GGG	0.621855	3_1:+66597009	None:intergenic
AGGTGCCAAAAGGCATAGGA+AGG	0.632227	3_1:-66596833	Msa0369400:CDS
TCAACCTCCAAACCCATAGA+GGG	0.633837	3_1:+66596585	None:intergenic
CAAAGCATAGACAACATCCA+TGG	0.634439	3_1:+66596624	None:intergenic
ATGTCTGGTCGTGGTAAGGG+AGG	0.640958	3_1:-66596874	Msa0369400:CDS
GGAGTGGGATAAGAGAGAAG+GGG	0.647754	3_1:+66596970	None:intergenic
TGAGAGCGGGATGAATAAGA+GGG	0.647912	3_1:+66597022	None:intergenic
GTGCTTCGCGATAACATCCA+GGG	0.673472	3_1:-66596811	Msa0369400:CDS
ATGTTGTCTATGCTTTGAAG+AGG	0.684273	3_1:-66596618	Msa0369400:CDS
TTGATCTATGAAGAAACCAG+AGG	0.704889	3_1:-66596727	Msa0369400:CDS
CATACACTGAGCATGCAAGG+AGG	0.758591	3_1:-66596660	Msa0369400:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAAAAAATAAGCATATTATA+AGG	+	chr3_1:66596789-66596808	None:intergenic	10.0%
!!!	ATTAATTTTGTAACATTAAA+AGG	-	chr3_1:66597010-66597029	Msa0369400:five_prime_UTR	10.0%
!!!	TTAATTTTGTAACATTAAAA+GGG	-	chr3_1:66597011-66597030	Msa0369400:five_prime_UTR	10.0%
!!	TAAAAAATAAGCATATTATA+AGG	+	chr3_1:66596789-66596808	None:intergenic	10.0%
!!!	ATTAATTTTGTAACATTAAA+AGG	-	chr3_1:66597010-66597029	Msa0369400:five_prime_UTR	10.0%
!!!	TTAATTTTGTAACATTAAAA+GGG	-	chr3_1:66597011-66597030	Msa0369400:five_prime_UTR	10.0%
!!!	TAATATGCTTATTTTTTACT+TGG	-	chr3_1:66596791-66596810	Msa0369400:CDS	15.0%
!!	TAAAAAAGAAAACATAAACT+AGG	+	chr3_1:66596885-66596904	None:intergenic	15.0%
!!!	TAATATGCTTATTTTTTACT+TGG	-	chr3_1:66596791-66596810	Msa0369400:CDS	15.0%
!!	TAAAAAAGAAAACATAAACT+AGG	+	chr3_1:66596885-66596904	None:intergenic	15.0%
!!	TGAGAAATTTGAAGAAATTA+GGG	+	chr3_1:66596332-66596351	None:intergenic	20.0%
!!	TTGAGAAATTTGAAGAAATT+AGG	+	chr3_1:66596333-66596352	None:intergenic	20.0%
!!	ATTATTGTTTAGCTAGTAAT+TGG	-	chr3_1:66596749-66596768	Msa0369400:CDS	20.0%
!!	TGCAATTTAGTATAATCATA+CGG	-	chr3_1:66596911-66596930	Msa0369400:five_prime_UTR	20.0%
!!	TGAGAAATTTGAAGAAATTA+GGG	+	chr3_1:66596332-66596351	None:intergenic	20.0%
!!	TTGAGAAATTTGAAGAAATT+AGG	+	chr3_1:66596333-66596352	None:intergenic	20.0%
!!	ATTATTGTTTAGCTAGTAAT+TGG	-	chr3_1:66596749-66596768	Msa0369400:CDS	20.0%
!!	TGCAATTTAGTATAATCATA+CGG	-	chr3_1:66596911-66596930	Msa0369400:five_prime_UTR	20.0%
!	AATTTGAAGAAATTAGGGTT+TGG	+	chr3_1:66596327-66596346	None:intergenic	25.0%
!	ATGTCATGTACTTAACAATT+TGG	+	chr3_1:66596992-66597011	None:intergenic	25.0%
!	AATTTGAAGAAATTAGGGTT+TGG	+	chr3_1:66596327-66596346	None:intergenic	25.0%
!	ATGTCATGTACTTAACAATT+TGG	+	chr3_1:66596992-66597011	None:intergenic	25.0%
!!!	ATTAGGGTTTGGTTTTGAAT+TGG	+	chr3_1:66596316-66596335	None:intergenic	30.0%
	TTTCATCTCTTGAAAATGTC+TGG	-	chr3_1:66596373-66596392	Msa0369400:three_prime_UTR	30.0%
	TCTAATGAAGTAATCGTTTC+CGG	-	chr3_1:66596848-66596867	Msa0369400:CDS	30.0%
!!!	ATTAGGGTTTGGTTTTGAAT+TGG	+	chr3_1:66596316-66596335	None:intergenic	30.0%
	TTTCATCTCTTGAAAATGTC+TGG	-	chr3_1:66596373-66596392	Msa0369400:three_prime_UTR	30.0%
	TCTAATGAAGTAATCGTTTC+CGG	-	chr3_1:66596848-66596867	Msa0369400:CDS	30.0%
	GGGATCTGAGTATTTATGAA+TGG	+	chr3_1:66596275-66596294	None:intergenic	35.0%
!!!	GTTTGGTTTTGAATTGGAGT+GGG	+	chr3_1:66596310-66596329	None:intergenic	35.0%
	TTGATCTATGAAGAAACCAG+AGG	-	chr3_1:66596535-66596554	Msa0369400:three_prime_UTR	35.0%
!!	ATGTTGTCTATGCTTTGAAG+AGG	-	chr3_1:66596644-66596663	Msa0369400:CDS	35.0%
	GGGATCTGAGTATTTATGAA+TGG	+	chr3_1:66596275-66596294	None:intergenic	35.0%
!!!	GTTTGGTTTTGAATTGGAGT+GGG	+	chr3_1:66596310-66596329	None:intergenic	35.0%
	TTGATCTATGAAGAAACCAG+AGG	-	chr3_1:66596535-66596554	Msa0369400:three_prime_UTR	35.0%
!!	ATGTTGTCTATGCTTTGAAG+AGG	-	chr3_1:66596644-66596663	Msa0369400:CDS	35.0%
!!!	GGTTTGGTTTTGAATTGGAG+TGG	+	chr3_1:66596311-66596330	None:intergenic	40.0%
	AAAATGTCTGGTCGTGGTAA+GGG	-	chr3_1:66596385-66596404	Msa0369400:three_prime_UTR	40.0%
	AGGTGTCTTGAAGATCTTCT+TGG	-	chr3_1:66596555-66596574	Msa0369400:intron	40.0%
	TTACATACACTGAGCATGCA+AGG	-	chr3_1:66596599-66596618	Msa0369400:CDS	40.0%
	CAAAGCATAGACAACATCCA+TGG	+	chr3_1:66596641-66596660	None:intergenic	40.0%
	TAGCTAGTAATTGGAACTCG+TGG	-	chr3_1:66596758-66596777	Msa0369400:CDS	40.0%
!	AAACTAGGAGCATAGTTCAC+CGG	+	chr3_1:66596870-66596889	None:intergenic	40.0%
!!!	GGTTTGGTTTTGAATTGGAG+TGG	+	chr3_1:66596311-66596330	None:intergenic	40.0%
	AAAATGTCTGGTCGTGGTAA+GGG	-	chr3_1:66596385-66596404	Msa0369400:three_prime_UTR	40.0%
	AGGTGTCTTGAAGATCTTCT+TGG	-	chr3_1:66596555-66596574	Msa0369400:intron	40.0%
	TTACATACACTGAGCATGCA+AGG	-	chr3_1:66596599-66596618	Msa0369400:CDS	40.0%
	CAAAGCATAGACAACATCCA+TGG	+	chr3_1:66596641-66596660	None:intergenic	40.0%
	TAGCTAGTAATTGGAACTCG+TGG	-	chr3_1:66596758-66596777	Msa0369400:CDS	40.0%
!	AAACTAGGAGCATAGTTCAC+CGG	+	chr3_1:66596870-66596889	None:intergenic	40.0%
	TGAGAGCGGGATGAATAAGA+GGG	+	chr3_1:66596243-66596262	None:intergenic	45.0%
	TGGAGTGGGATAAGAGAGAA+GGG	+	chr3_1:66596296-66596315	None:intergenic	45.0%
	TTGGAGTGGGATAAGAGAGA+AGG	+	chr3_1:66596297-66596316	None:intergenic	45.0%
	CTCTTGAAAATGTCTGGTCG+TGG	-	chr3_1:66596379-66596398	Msa0369400:three_prime_UTR	45.0%
	GAAAATGTCTGGTCGTGGTA+AGG	-	chr3_1:66596384-66596403	Msa0369400:three_prime_UTR	45.0%
!	AAGCACCTTCCTATGCCTTT+TGG	+	chr3_1:66596437-66596456	None:intergenic	45.0%
	GAAGATCTTCAAGACACCTC+TGG	+	chr3_1:66596554-66596573	None:intergenic	45.0%
!!	TGTCTATGCTTTGAAGAGGC+AGG	-	chr3_1:66596648-66596667	Msa0369400:CDS	45.0%
!!	GTCTATGCTTTGAAGAGGCA+GGG	-	chr3_1:66596649-66596668	Msa0369400:CDS	45.0%
	TCAACCTCCAAACCCATAGA+GGG	+	chr3_1:66596680-66596699	None:intergenic	45.0%
	ATCAACCTCCAAACCCATAG+AGG	+	chr3_1:66596681-66596700	None:intergenic	45.0%
	TGAGAGCGGGATGAATAAGA+GGG	+	chr3_1:66596243-66596262	None:intergenic	45.0%
	TGGAGTGGGATAAGAGAGAA+GGG	+	chr3_1:66596296-66596315	None:intergenic	45.0%
	TTGGAGTGGGATAAGAGAGA+AGG	+	chr3_1:66596297-66596316	None:intergenic	45.0%
	CTCTTGAAAATGTCTGGTCG+TGG	-	chr3_1:66596379-66596398	Msa0369400:three_prime_UTR	45.0%
	GAAAATGTCTGGTCGTGGTA+AGG	-	chr3_1:66596384-66596403	Msa0369400:three_prime_UTR	45.0%
!	AAGCACCTTCCTATGCCTTT+TGG	+	chr3_1:66596437-66596456	None:intergenic	45.0%
	GAAGATCTTCAAGACACCTC+TGG	+	chr3_1:66596554-66596573	None:intergenic	45.0%
!!	TGTCTATGCTTTGAAGAGGC+AGG	-	chr3_1:66596648-66596667	Msa0369400:CDS	45.0%
!!	GTCTATGCTTTGAAGAGGCA+GGG	-	chr3_1:66596649-66596668	Msa0369400:CDS	45.0%
	TCAACCTCCAAACCCATAGA+GGG	+	chr3_1:66596680-66596699	None:intergenic	45.0%
	ATCAACCTCCAAACCCATAG+AGG	+	chr3_1:66596681-66596700	None:intergenic	45.0%
	GTGAGAGCGGGATGAATAAG+AGG	+	chr3_1:66596244-66596263	None:intergenic	50.0%
!	AATGGTAGTGAGCGTGAGAG+CGG	+	chr3_1:66596257-66596276	None:intergenic	50.0%
	GGAGTGGGATAAGAGAGAAG+GGG	+	chr3_1:66596295-66596314	None:intergenic	50.0%
	GGAGGTAAGGGACTAGGAAA+GGG	-	chr3_1:66596406-66596425	Msa0369400:three_prime_UTR	50.0%
!!	TAGGAAAGGGAGGTGCCAAA+AGG	-	chr3_1:66596419-66596438	Msa0369400:three_prime_UTR	50.0%
!	AGGTGCCAAAAGGCATAGGA+AGG	-	chr3_1:66596429-66596448	Msa0369400:three_prime_UTR	50.0%
	GTGCTTCGCGATAACATCCA+GGG	-	chr3_1:66596451-66596470	Msa0369400:three_prime_UTR	50.0%
	TAAGCCTGCGATTCGTCGTT+TGG	-	chr3_1:66596480-66596499	Msa0369400:three_prime_UTR	50.0%
	ATTCGTCGTTTGGCGAGAAG+AGG	-	chr3_1:66596490-66596509	Msa0369400:three_prime_UTR	50.0%
	TTCGTCGTTTGGCGAGAAGA+GGG	-	chr3_1:66596491-66596510	Msa0369400:three_prime_UTR	50.0%
	CATACACTGAGCATGCAAGG+AGG	-	chr3_1:66596602-66596621	Msa0369400:CDS	50.0%
	GGAAGAACCCTCTATGGGTT+TGG	-	chr3_1:66596670-66596689	Msa0369400:CDS	50.0%
	AGAACCCTCTATGGGTTTGG+AGG	-	chr3_1:66596673-66596692	Msa0369400:CDS	50.0%
	GTGAGAGCGGGATGAATAAG+AGG	+	chr3_1:66596244-66596263	None:intergenic	50.0%
!	AATGGTAGTGAGCGTGAGAG+CGG	+	chr3_1:66596257-66596276	None:intergenic	50.0%
	GGAGTGGGATAAGAGAGAAG+GGG	+	chr3_1:66596295-66596314	None:intergenic	50.0%
	GGAGGTAAGGGACTAGGAAA+GGG	-	chr3_1:66596406-66596425	Msa0369400:three_prime_UTR	50.0%
!!	TAGGAAAGGGAGGTGCCAAA+AGG	-	chr3_1:66596419-66596438	Msa0369400:three_prime_UTR	50.0%
!	AGGTGCCAAAAGGCATAGGA+AGG	-	chr3_1:66596429-66596448	Msa0369400:three_prime_UTR	50.0%
	GTGCTTCGCGATAACATCCA+GGG	-	chr3_1:66596451-66596470	Msa0369400:three_prime_UTR	50.0%
	TAAGCCTGCGATTCGTCGTT+TGG	-	chr3_1:66596480-66596499	Msa0369400:three_prime_UTR	50.0%
	ATTCGTCGTTTGGCGAGAAG+AGG	-	chr3_1:66596490-66596509	Msa0369400:three_prime_UTR	50.0%
	TTCGTCGTTTGGCGAGAAGA+GGG	-	chr3_1:66596491-66596510	Msa0369400:three_prime_UTR	50.0%
	CATACACTGAGCATGCAAGG+AGG	-	chr3_1:66596602-66596621	Msa0369400:CDS	50.0%
	GGAAGAACCCTCTATGGGTT+TGG	-	chr3_1:66596670-66596689	Msa0369400:CDS	50.0%
	AGAACCCTCTATGGGTTTGG+AGG	-	chr3_1:66596673-66596692	Msa0369400:CDS	50.0%
!	ATGGTAGTGAGCGTGAGAGC+GGG	+	chr3_1:66596256-66596275	None:intergenic	55.0%
	ATGTCTGGTCGTGGTAAGGG+AGG	-	chr3_1:66596388-66596407	Msa0369400:three_prime_UTR	55.0%
	TGGTCGTGGTAAGGGAGGTA+AGG	-	chr3_1:66596393-66596412	Msa0369400:three_prime_UTR	55.0%
	GGTCGTGGTAAGGGAGGTAA+GGG	-	chr3_1:66596394-66596413	Msa0369400:three_prime_UTR	55.0%
	GGTAAGGGAGGTAAGGGACT+AGG	-	chr3_1:66596400-66596419	Msa0369400:three_prime_UTR	55.0%
	GGGAGGTAAGGGACTAGGAA+AGG	-	chr3_1:66596405-66596424	Msa0369400:three_prime_UTR	55.0%
	GGTAAGGGACTAGGAAAGGG+AGG	-	chr3_1:66596409-66596428	Msa0369400:three_prime_UTR	55.0%
!!	AGGGAGGTGCCAAAAGGCAT+AGG	-	chr3_1:66596425-66596444	Msa0369400:three_prime_UTR	55.0%
	GGTGCTTCGCGATAACATCC+AGG	-	chr3_1:66596450-66596469	Msa0369400:three_prime_UTR	55.0%
	TCGCAGGCTTAGTGATACCC+TGG	+	chr3_1:66596471-66596490	None:intergenic	55.0%
	TCGTCGTTTGGCGAGAAGAG+GGG	-	chr3_1:66596492-66596511	Msa0369400:three_prime_UTR	55.0%
	GAGGAAGACTGTGACTGCCA+TGG	-	chr3_1:66596621-66596640	Msa0369400:CDS	55.0%
	AGGCAGGGAAGAACCCTCTA+TGG	-	chr3_1:66596664-66596683	Msa0369400:CDS	55.0%
	GGCAGGGAAGAACCCTCTAT+GGG	-	chr3_1:66596665-66596684	Msa0369400:CDS	55.0%
!	ATGGTAGTGAGCGTGAGAGC+GGG	+	chr3_1:66596256-66596275	None:intergenic	55.0%
	ATGTCTGGTCGTGGTAAGGG+AGG	-	chr3_1:66596388-66596407	Msa0369400:three_prime_UTR	55.0%
	TGGTCGTGGTAAGGGAGGTA+AGG	-	chr3_1:66596393-66596412	Msa0369400:three_prime_UTR	55.0%
	GGTCGTGGTAAGGGAGGTAA+GGG	-	chr3_1:66596394-66596413	Msa0369400:three_prime_UTR	55.0%
	GGTAAGGGAGGTAAGGGACT+AGG	-	chr3_1:66596400-66596419	Msa0369400:three_prime_UTR	55.0%
	GGGAGGTAAGGGACTAGGAA+AGG	-	chr3_1:66596405-66596424	Msa0369400:three_prime_UTR	55.0%
	GGTAAGGGACTAGGAAAGGG+AGG	-	chr3_1:66596409-66596428	Msa0369400:three_prime_UTR	55.0%
!!	AGGGAGGTGCCAAAAGGCAT+AGG	-	chr3_1:66596425-66596444	Msa0369400:three_prime_UTR	55.0%
	GGTGCTTCGCGATAACATCC+AGG	-	chr3_1:66596450-66596469	Msa0369400:three_prime_UTR	55.0%
	TCGCAGGCTTAGTGATACCC+TGG	+	chr3_1:66596471-66596490	None:intergenic	55.0%
	TCGTCGTTTGGCGAGAAGAG+GGG	-	chr3_1:66596492-66596511	Msa0369400:three_prime_UTR	55.0%
	GAGGAAGACTGTGACTGCCA+TGG	-	chr3_1:66596621-66596640	Msa0369400:CDS	55.0%
	AGGCAGGGAAGAACCCTCTA+TGG	-	chr3_1:66596664-66596683	Msa0369400:CDS	55.0%
	GGCAGGGAAGAACCCTCTAT+GGG	-	chr3_1:66596665-66596684	Msa0369400:CDS	55.0%
	CTCGCCAAACGACGAATCGC+AGG	+	chr3_1:66596487-66596506	None:intergenic	60.0%
	CGTCGTTTGGCGAGAAGAGG+GGG	-	chr3_1:66596493-66596512	Msa0369400:three_prime_UTR	60.0%
!	CGAGAAGAGGGGGTGTGAAG+AGG	-	chr3_1:66596503-66596522	Msa0369400:three_prime_UTR	60.0%
!	GGGGGTGTGAAGAGGATCAG+TGG	-	chr3_1:66596511-66596530	Msa0369400:three_prime_UTR	60.0%
	CTCGCCAAACGACGAATCGC+AGG	+	chr3_1:66596487-66596506	None:intergenic	60.0%
	CGTCGTTTGGCGAGAAGAGG+GGG	-	chr3_1:66596493-66596512	Msa0369400:three_prime_UTR	60.0%
!	CGAGAAGAGGGGGTGTGAAG+AGG	-	chr3_1:66596503-66596522	Msa0369400:three_prime_UTR	60.0%
!	GGGGGTGTGAAGAGGATCAG+TGG	-	chr3_1:66596511-66596530	Msa0369400:three_prime_UTR	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3_1	gene	66596234	66597050	66596234	ID=Msa0369400;Name=Msa0369400
chr3_1	mRNA	66596234	66597050	66596234	ID=Msa0369400-mRNA-1;Parent=Msa0369400;Name=Msa0369400-mRNA-1;_AED=0.00;_eAED=0.00;_QI=154\|0\|0.5\|1\|0\|0.5\|2\|332\|103
chr3_1	exon	66596234	66596565	66596234	ID=Msa0369400-mRNA-1:exon:13595;Parent=Msa0369400-mRNA-1
chr3_1	exon	66596585	66597050	66596585	ID=Msa0369400-mRNA-1:exon:13594;Parent=Msa0369400-mRNA-1
chr3_1	five_prime_UTR	66596897	66597050	66596897	ID=Msa0369400-mRNA-1:five_prime_utr;Parent=Msa0369400-mRNA-1
chr3_1	CDS	66596585	66596896	66596585	ID=Msa0369400-mRNA-1:cds;Parent=Msa0369400-mRNA-1
chr3_1	three_prime_UTR	66596234	66596565	66596234	ID=Msa0369400-mRNA-1:three_prime_utr;Parent=Msa0369400-mRNA-1

Gene Sequence	>Msa0369400 ATGTCTGGTCGTGGTAAGGGAGGTAAGGGACTAGGAAAGGGAGGTGCCAAAAGGCATAGGAAGGTGCTTCGCGATAACATCCAGGGTATCACTAAGCCTGCGATTCGTCGTTTGGCGAGAAGAGGGGGTGTGAAGAGGATCAGTGGTTTGATCTATGAAGAAACCAGAGGTGTCTTGAAGATCTTCTTGGAGAATGTCATTCGTGACGCAGTTACATACACTGAGCATGCAAGGAGGAAGACTGTGACTGCCATGGATGTTGTCTATGCTTTGAAGAGGCAGGGAAGAACCCTCTATGGGTTTGGAGGTTGA
Protein sequence	>Msa0369400 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG*