AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0697160


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0697160 MtrunA17_Chr4g0049791 98.824 85 1 0 1 85 1 85 3.18e-58 172
Msa0697160 MtrunA17_Chr2g0279651 85.882 85 12 0 1 85 1 85 7.55e-51 154
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0697160 AT4G33640.1 80.769 78 15 0 1 78 1 78 1.13e-41 132
Msa0697160 AT4G33640.2 80.769 78 15 0 1 78 67 144 3.49e-41 132

Find 31 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTTGATTCACAAACTAAA+TGG 0.258730 4_4:-67148549 None:intergenic
TGCATTTACTAAAACAGATT+TGG 0.294396 4_4:+67148720 Msa0697160:three_prime_UTR
TGCATTGTGAAGGTCACATT+TGG 0.398089 4_4:+67148213 Msa0697160:intron
AGTTGATGCTTAAATTTCAA+TGG 0.407398 4_4:-67148676 None:intergenic
CCTGCTGCTGCTAACTGATT+CGG 0.442731 4_4:+67148387 Msa0697160:exon
GAGTGATTTCCACATTATCA+TGG 0.446001 4_4:-67148353 None:intergenic
GTGTGTTTAAAGTTTCTGTT+CGG 0.456320 4_4:-67147413 None:intergenic
GAACACTCTTTCACGATGAT+CGG 0.491735 4_4:+67148235 Msa0697160:CDS
AAGAGGAGGTTGAAAAGCTG+AGG 0.496528 4_4:+67147498 Msa0697160:CDS
CCGAATCAGTTAGCAGCAGC+AGG 0.502169 4_4:-67148387 None:intergenic
CAGTTAGCAGCAGCAGGAGC+AGG 0.504867 4_4:-67148381 None:intergenic
GTTAGTGATTCAAGCAGTAA+AGG 0.512252 4_4:-67148431 None:intergenic
CTTGGAAATGTTCAAGCAGA+TGG 0.516658 4_4:+67147536 Msa0697160:CDS
CACTTCGTAAATAATAGTAT+AGG 0.525059 4_4:-67148644 None:intergenic
AGGGAAGAAATCAAGAGGCT+TGG 0.527446 4_4:+67147518 Msa0697160:CDS
CTTAAATTTCAATGGTTCTG+TGG 0.531142 4_4:-67148668 None:intergenic
GCTAACTGATTCGGTTGTGA+AGG 0.539593 4_4:+67148396 Msa0697160:exon
TGTGAAGATGAACGTAGAAG+AGG 0.563453 4_4:+67147481 Msa0697160:exon
GAAGATGAACGTAGAAGAGG+AGG 0.565552 4_4:+67147484 Msa0697160:exon
AGAGGAGGTTGAAAAGCTGA+GGG 0.572997 4_4:+67147499 Msa0697160:CDS
ATAGTATAGGATAAACATGA+AGG 0.579841 4_4:-67148631 None:intergenic
CTATACTATTATTTACGAAG+TGG 0.588267 4_4:+67148645 Msa0697160:three_prime_UTR
AATATATTTGAAGCACTCGT+CGG 0.601303 4_4:+67148264 Msa0697160:CDS
TCAAGCAGATGGTTCTTGCA+AGG 0.604584 4_4:+67147547 Msa0697160:CDS
CTAACTGATTCGGTTGTGAA+GGG 0.617075 4_4:+67148397 Msa0697160:exon
AGCTGAGGGAAGAAATCAAG+AGG 0.626324 4_4:+67147513 Msa0697160:CDS
GACGGTGAACTACTGTTGCA+AGG 0.632747 4_4:+67148327 Msa0697160:CDS
GATGTTGTAATATATGCATG+TGG 0.675462 4_4:+67148489 Msa0697160:three_prime_UTR
GCAAGGAGTCCATGATAATG+TGG 0.676630 4_4:+67148344 Msa0697160:CDS
TGTAATATATGCATGTGGAG+AGG 0.735312 4_4:+67148494 Msa0697160:three_prime_UTR
CGTAAAGTAGTTGCATACGA+CGG 0.749247 4_4:+67148309 Msa0697160:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAAATATAAAAAGCTTTT+AGG + chr4_4:67147390-67147409 Msa0697160:five_prime_UTR 10.0%
!! TAATAAACAAATTAATGTAT+AGG - chr4_4:67147859-67147878 None:intergenic 10.0%
!!! AATAAATATAAAAAGCTTTT+AGG + chr4_4:67147390-67147409 Msa0697160:five_prime_UTR 10.0%
!! TAATAAACAAATTAATGTAT+AGG - chr4_4:67147859-67147878 None:intergenic 10.0%
!!! AATTTGTTTATTATAAACCA+TGG + chr4_4:67147866-67147885 Msa0697160:intron 15.0%
!! ATAATTGTAATTAAATGTGT+TGG + chr4_4:67148028-67148047 Msa0697160:intron 15.0%
!!! AATTTGTTTATTATAAACCA+TGG + chr4_4:67147866-67147885 Msa0697160:intron 15.0%
!! ATAATTGTAATTAAATGTGT+TGG + chr4_4:67148028-67148047 Msa0697160:intron 15.0%
!! GAATCAAGTTCTTAATTAAA+AGG - chr4_4:67147653-67147672 None:intergenic 20.0%
!!! AAGTATTGATATGATGTTTA+GGG - chr4_4:67147993-67148012 None:intergenic 20.0%
!!! TAAGTATTGATATGATGTTT+AGG - chr4_4:67147994-67148013 None:intergenic 20.0%
!! GAATCAAGTTCTTAATTAAA+AGG - chr4_4:67147653-67147672 None:intergenic 20.0%
!!! AAGTATTGATATGATGTTTA+GGG - chr4_4:67147993-67148012 None:intergenic 20.0%
!!! TAAGTATTGATATGATGTTT+AGG - chr4_4:67147994-67148013 None:intergenic 20.0%
!!! AAGAAAGACTTTCTTGTTTT+TGG + chr4_4:67147439-67147458 Msa0697160:five_prime_UTR 25.0%
!! GAAAATGTATATGGTTTGTA+AGG - chr4_4:67147606-67147625 None:intergenic 25.0%
! ATGCATCATGAAAATGTATA+TGG - chr4_4:67147615-67147634 None:intergenic 25.0%
! TTCAGATCTAGATATATTGA+TGG + chr4_4:67147670-67147689 Msa0697160:intron 25.0%
! TCAGATCTAGATATATTGAT+GGG + chr4_4:67147671-67147690 Msa0697160:intron 25.0%
! TAATGTATAGGTAGATACAT+TGG - chr4_4:67147847-67147866 None:intergenic 25.0%
! TTGTTTATTATAAACCATGG+AGG + chr4_4:67147869-67147888 Msa0697160:intron 25.0%
!!! CATATCAGTTTTATATGGAA+TGG - chr4_4:67147914-67147933 None:intergenic 25.0%
! CATTCCATATAAAACTGATA+TGG + chr4_4:67147912-67147931 Msa0697160:intron 25.0%
!!! AATTTGCTGAAATAGTACTA+TGG + chr4_4:67148088-67148107 Msa0697160:intron 25.0%
! AAATATGTAATGCATTGTGA+AGG + chr4_4:67148203-67148222 Msa0697160:intron 25.0%
! CATTTGATTCACAAACTAAA+TGG - chr4_4:67148552-67148571 None:intergenic 25.0%
! ATAGTATAGGATAAACATGA+AGG - chr4_4:67148634-67148653 None:intergenic 25.0%
! CACTTCGTAAATAATAGTAT+AGG - chr4_4:67148647-67148666 None:intergenic 25.0%
! CTATACTATTATTTACGAAG+TGG + chr4_4:67148645-67148664 Msa0697160:three_prime_UTR 25.0%
!! AGTTGATGCTTAAATTTCAA+TGG - chr4_4:67148679-67148698 None:intergenic 25.0%
! TGCATTTACTAAAACAGATT+TGG + chr4_4:67148720-67148739 Msa0697160:three_prime_UTR 25.0%
!!! AAGAAAGACTTTCTTGTTTT+TGG + chr4_4:67147439-67147458 Msa0697160:five_prime_UTR 25.0%
!! GAAAATGTATATGGTTTGTA+AGG - chr4_4:67147606-67147625 None:intergenic 25.0%
! ATGCATCATGAAAATGTATA+TGG - chr4_4:67147615-67147634 None:intergenic 25.0%
! TTCAGATCTAGATATATTGA+TGG + chr4_4:67147670-67147689 Msa0697160:intron 25.0%
! TCAGATCTAGATATATTGAT+GGG + chr4_4:67147671-67147690 Msa0697160:intron 25.0%
! TAATGTATAGGTAGATACAT+TGG - chr4_4:67147847-67147866 None:intergenic 25.0%
! TTGTTTATTATAAACCATGG+AGG + chr4_4:67147869-67147888 Msa0697160:intron 25.0%
!!! CATATCAGTTTTATATGGAA+TGG - chr4_4:67147914-67147933 None:intergenic 25.0%
! CATTCCATATAAAACTGATA+TGG + chr4_4:67147912-67147931 Msa0697160:intron 25.0%
!!! AATTTGCTGAAATAGTACTA+TGG + chr4_4:67148088-67148107 Msa0697160:intron 25.0%
! AAATATGTAATGCATTGTGA+AGG + chr4_4:67148203-67148222 Msa0697160:intron 25.0%
! CATTTGATTCACAAACTAAA+TGG - chr4_4:67148552-67148571 None:intergenic 25.0%
! ATAGTATAGGATAAACATGA+AGG - chr4_4:67148634-67148653 None:intergenic 25.0%
! CACTTCGTAAATAATAGTAT+AGG - chr4_4:67148647-67148666 None:intergenic 25.0%
! CTATACTATTATTTACGAAG+TGG + chr4_4:67148645-67148664 Msa0697160:three_prime_UTR 25.0%
!! AGTTGATGCTTAAATTTCAA+TGG - chr4_4:67148679-67148698 None:intergenic 25.0%
! TGCATTTACTAAAACAGATT+TGG + chr4_4:67148720-67148739 Msa0697160:three_prime_UTR 25.0%
! GTGTGTTTAAAGTTTCTGTT+CGG - chr4_4:67147416-67147435 None:intergenic 30.0%
!!! AAAGACTTTCTTGTTTTTGG+TGG + chr4_4:67147442-67147461 Msa0697160:five_prime_UTR 30.0%
TTCTTGCAAGGTATAACAAA+AGG + chr4_4:67147559-67147578 Msa0697160:intron 30.0%
! TGATGGGTATTTATTGAATG+TGG + chr4_4:67147687-67147706 Msa0697160:intron 30.0%
! TGGTCACTTTCTTATTCTTA+TGG + chr4_4:67147707-67147726 Msa0697160:intron 30.0%
!! CTAGCCATATCAGTTTTATA+TGG - chr4_4:67147919-67147938 None:intergenic 30.0%
! CTATATCTTCAGTTGATTCA+TGG - chr4_4:67148123-67148142 None:intergenic 30.0%
! ATAGTGAGTGATTGGTTAAT+TGG + chr4_4:67148139-67148158 Msa0697160:intron 30.0%
! AATATATTTGAAGCACTCGT+CGG + chr4_4:67148264-67148283 Msa0697160:CDS 30.0%
!!! GAATCACTAACCTGTTTTTA+TGG + chr4_4:67148443-67148462 Msa0697160:three_prime_UTR 30.0%
GATGTTGTAATATATGCATG+TGG + chr4_4:67148489-67148508 Msa0697160:three_prime_UTR 30.0%
! CTTAAATTTCAATGGTTCTG+TGG - chr4_4:67148671-67148690 None:intergenic 30.0%
! GTGTGTTTAAAGTTTCTGTT+CGG - chr4_4:67147416-67147435 None:intergenic 30.0%
!!! AAAGACTTTCTTGTTTTTGG+TGG + chr4_4:67147442-67147461 Msa0697160:five_prime_UTR 30.0%
TTCTTGCAAGGTATAACAAA+AGG + chr4_4:67147559-67147578 Msa0697160:intron 30.0%
! TGATGGGTATTTATTGAATG+TGG + chr4_4:67147687-67147706 Msa0697160:intron 30.0%
! TGGTCACTTTCTTATTCTTA+TGG + chr4_4:67147707-67147726 Msa0697160:intron 30.0%
!! CTAGCCATATCAGTTTTATA+TGG - chr4_4:67147919-67147938 None:intergenic 30.0%
! CTATATCTTCAGTTGATTCA+TGG - chr4_4:67148123-67148142 None:intergenic 30.0%
! ATAGTGAGTGATTGGTTAAT+TGG + chr4_4:67148139-67148158 Msa0697160:intron 30.0%
! AATATATTTGAAGCACTCGT+CGG + chr4_4:67148264-67148283 Msa0697160:CDS 30.0%
!!! GAATCACTAACCTGTTTTTA+TGG + chr4_4:67148443-67148462 Msa0697160:three_prime_UTR 30.0%
GATGTTGTAATATATGCATG+TGG + chr4_4:67148489-67148508 Msa0697160:three_prime_UTR 30.0%
! CTTAAATTTCAATGGTTCTG+TGG - chr4_4:67148671-67148690 None:intergenic 30.0%
TCTTATGGATCTAGATCACA+AGG + chr4_4:67147722-67147741 Msa0697160:intron 35.0%
CTGAAGATATAGTGAGTGAT+TGG + chr4_4:67148131-67148150 Msa0697160:intron 35.0%
GAGTGATTTCCACATTATCA+TGG - chr4_4:67148356-67148375 None:intergenic 35.0%
GTTAGTGATTCAAGCAGTAA+AGG - chr4_4:67148434-67148453 None:intergenic 35.0%
TGTAATATATGCATGTGGAG+AGG + chr4_4:67148494-67148513 Msa0697160:three_prime_UTR 35.0%
TCTTATGGATCTAGATCACA+AGG + chr4_4:67147722-67147741 Msa0697160:intron 35.0%
CTGAAGATATAGTGAGTGAT+TGG + chr4_4:67148131-67148150 Msa0697160:intron 35.0%
GAGTGATTTCCACATTATCA+TGG - chr4_4:67148356-67148375 None:intergenic 35.0%
GTTAGTGATTCAAGCAGTAA+AGG - chr4_4:67148434-67148453 None:intergenic 35.0%
TGTAATATATGCATGTGGAG+AGG + chr4_4:67148494-67148513 Msa0697160:three_prime_UTR 35.0%
TGTGAAGATGAACGTAGAAG+AGG + chr4_4:67147481-67147500 Msa0697160:exon 40.0%
CTTGGAAATGTTCAAGCAGA+TGG + chr4_4:67147536-67147555 Msa0697160:CDS 40.0%
CACAAGGTCATTCACAACAT+TGG + chr4_4:67147738-67147757 Msa0697160:intron 40.0%
TGCATTGTGAAGGTCACATT+TGG + chr4_4:67148213-67148232 Msa0697160:intron 40.0%
GAACACTCTTTCACGATGAT+CGG + chr4_4:67148235-67148254 Msa0697160:CDS 40.0%
CGTAAAGTAGTTGCATACGA+CGG + chr4_4:67148309-67148328 Msa0697160:CDS 40.0%
!! CTAACTGATTCGGTTGTGAA+GGG + chr4_4:67148397-67148416 Msa0697160:exon 40.0%
!!! CACTAACCTGTTTTTATGGC+TGG + chr4_4:67148447-67148466 Msa0697160:three_prime_UTR 40.0%
ATCACACCAGCCATAAAAAC+AGG - chr4_4:67148456-67148475 None:intergenic 40.0%
TGTGAAGATGAACGTAGAAG+AGG + chr4_4:67147481-67147500 Msa0697160:exon 40.0%
CTTGGAAATGTTCAAGCAGA+TGG + chr4_4:67147536-67147555 Msa0697160:CDS 40.0%
CACAAGGTCATTCACAACAT+TGG + chr4_4:67147738-67147757 Msa0697160:intron 40.0%
TGCATTGTGAAGGTCACATT+TGG + chr4_4:67148213-67148232 Msa0697160:intron 40.0%
GAACACTCTTTCACGATGAT+CGG + chr4_4:67148235-67148254 Msa0697160:CDS 40.0%
CGTAAAGTAGTTGCATACGA+CGG + chr4_4:67148309-67148328 Msa0697160:CDS 40.0%
!! CTAACTGATTCGGTTGTGAA+GGG + chr4_4:67148397-67148416 Msa0697160:exon 40.0%
!!! CACTAACCTGTTTTTATGGC+TGG + chr4_4:67148447-67148466 Msa0697160:three_prime_UTR 40.0%
ATCACACCAGCCATAAAAAC+AGG - chr4_4:67148456-67148475 None:intergenic 40.0%
GAAGATGAACGTAGAAGAGG+AGG + chr4_4:67147484-67147503 Msa0697160:exon 45.0%
AAGAGGAGGTTGAAAAGCTG+AGG + chr4_4:67147498-67147517 Msa0697160:CDS 45.0%
AGAGGAGGTTGAAAAGCTGA+GGG + chr4_4:67147499-67147518 Msa0697160:CDS 45.0%
AGCTGAGGGAAGAAATCAAG+AGG + chr4_4:67147513-67147532 Msa0697160:CDS 45.0%
AGGGAAGAAATCAAGAGGCT+TGG + chr4_4:67147518-67147537 Msa0697160:CDS 45.0%
TCAAGCAGATGGTTCTTGCA+AGG + chr4_4:67147547-67147566 Msa0697160:CDS 45.0%
! GCAAGGAGTCCATGATAATG+TGG + chr4_4:67148344-67148363 Msa0697160:CDS 45.0%
!! GCTAACTGATTCGGTTGTGA+AGG + chr4_4:67148396-67148415 Msa0697160:exon 45.0%
GAAGATGAACGTAGAAGAGG+AGG + chr4_4:67147484-67147503 Msa0697160:exon 45.0%
AAGAGGAGGTTGAAAAGCTG+AGG + chr4_4:67147498-67147517 Msa0697160:CDS 45.0%
AGAGGAGGTTGAAAAGCTGA+GGG + chr4_4:67147499-67147518 Msa0697160:CDS 45.0%
AGCTGAGGGAAGAAATCAAG+AGG + chr4_4:67147513-67147532 Msa0697160:CDS 45.0%
AGGGAAGAAATCAAGAGGCT+TGG + chr4_4:67147518-67147537 Msa0697160:CDS 45.0%
TCAAGCAGATGGTTCTTGCA+AGG + chr4_4:67147547-67147566 Msa0697160:CDS 45.0%
! GCAAGGAGTCCATGATAATG+TGG + chr4_4:67148344-67148363 Msa0697160:CDS 45.0%
!! GCTAACTGATTCGGTTGTGA+AGG + chr4_4:67148396-67148415 Msa0697160:exon 45.0%
CGCATGATATCTCTCCTCCA+TGG - chr4_4:67147886-67147905 None:intergenic 50.0%
GACGGTGAACTACTGTTGCA+AGG + chr4_4:67148327-67148346 Msa0697160:CDS 50.0%
CCTGCTGCTGCTAACTGATT+CGG + chr4_4:67148387-67148406 Msa0697160:exon 50.0%
CGCATGATATCTCTCCTCCA+TGG - chr4_4:67147886-67147905 None:intergenic 50.0%
GACGGTGAACTACTGTTGCA+AGG + chr4_4:67148327-67148346 Msa0697160:CDS 50.0%
CCTGCTGCTGCTAACTGATT+CGG + chr4_4:67148387-67148406 Msa0697160:exon 50.0%
CCGAATCAGTTAGCAGCAGC+AGG - chr4_4:67148390-67148409 None:intergenic 55.0%
CCGAATCAGTTAGCAGCAGC+AGG - chr4_4:67148390-67148409 None:intergenic 55.0%
CAGTTAGCAGCAGCAGGAGC+AGG - chr4_4:67148384-67148403 None:intergenic 60.0%
CAGTTAGCAGCAGCAGGAGC+AGG - chr4_4:67148384-67148403 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 67147365 67148759 67147365 ID=Msa0697160;Name=Msa0697160
chr4_4 mRNA 67147365 67148759 67147365 ID=Msa0697160-mRNA-1;Parent=Msa0697160;Name=Msa0697160-mRNA-1;_AED=0.00;_eAED=0.00;_QI=123|1|1|1|1|1|2|355|86
chr4_4 exon 67147365 67147568 67147365 ID=Msa0697160-mRNA-1:exon:19602;Parent=Msa0697160-mRNA-1
chr4_4 exon 67148225 67148759 67148225 ID=Msa0697160-mRNA-1:exon:19603;Parent=Msa0697160-mRNA-1
chr4_4 five_prime_UTR 67147365 67147487 67147365 ID=Msa0697160-mRNA-1:five_prime_utr;Parent=Msa0697160-mRNA-1
chr4_4 CDS 67147488 67147568 67147488 ID=Msa0697160-mRNA-1:cds;Parent=Msa0697160-mRNA-1
chr4_4 CDS 67148225 67148404 67148225 ID=Msa0697160-mRNA-1:cds;Parent=Msa0697160-mRNA-1
chr4_4 three_prime_UTR 67148405 67148759 67148405 ID=Msa0697160-mRNA-1:three_prime_utr;Parent=Msa0697160-mRNA-1
Gene Sequence

>Msa0697160

ATGAACGTAGAAGAGGAGGTTGAAAAGCTGAGGGAAGAAATCAAGAGGCTTGGAAATGTTCAAGCAGATGGTTCTTGCAAGGTCACATTTGGAACACTCTTTCACGATGATCGGTGCGCAAATATATTTGAAGCACTCGTCGGTACGCTAAGAGCTGCTAAAAAGCGTAAAGTAGTTGCATACGACGGTGAACTACTGTTGCAAGGAGTCCATGATAATGTGGAAATCACTCTTAATCCTGCTCCTGCTGCTGCTAACTGA

Protein sequence

>Msa0697160

MNVEEEVEKLREEIKRLGNVQADGSCKVTFGTLFHDDRCANIFEALVGTLRAAKKRKVVAYDGELLLQGVHDNVEITLNPAPAAAN*