AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0577640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0577640 MtrunA17_Chr8g0377531 93.798 129 8 0 1 129 82 210 1.81e-83 244
Msa0577640 MtrunA17_Chr3g0122261 54.815 135 47 4 1 129 1 127 7.77e-42 136
Msa0577640 MtrunA17_Chr5g0434711 54.054 74 27 2 17 90 5 71 1.58e-16 70.1
Msa0577640 MtrunA17_Chr7g0224931 42.308 130 60 3 1 117 21 148 4.40e-16 71.2
Msa0577640 MtrunA17_Chr7g0224971 42.308 130 60 3 1 117 21 148 4.40e-16 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0577640 AT1G27290.2 54.762 126 48 4 1 119 1 124 8.33e-37 124
Msa0577640 AT1G27290.1 54.762 126 48 4 1 119 1 124 8.33e-37 124

Find 106 sgRNAs with CRISPR-Local

Find 179 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACAAGAAATAACTATTTC+TGG 0.134731 4_1:-81981042 None:intergenic
GAAGCCATGCCCACAAATTA+TGG 0.284897 4_1:-81980592 None:intergenic
TGTGGAGTTTGACATTATTA+TGG 0.287210 4_1:+81980104 Msa0577640:three_prime_UTR
AGAGAGGGAAAAGGGTATAA+AGG 0.293809 4_1:-81978560 None:intergenic
GATGTTATTATTGTTATAAC+AGG 0.298413 4_1:+81980914 Msa0577640:three_prime_UTR
GGATCGGTTACTTTCGTGAT+TGG 0.305883 4_1:+81979033 Msa0577640:CDS
CGTCTTTGAGTGGGAGGAGC+TGG 0.317023 4_1:+81981000 Msa0577640:three_prime_UTR
ATGTTGATGCCGTGGGTTAT+TGG 0.333475 4_1:+81979060 Msa0577640:CDS
CATATTACATGAAATAATTT+CGG 0.334699 4_1:-81980890 None:intergenic
TTACGTGAGGAATGGATAAT+TGG 0.347004 4_1:-81978483 None:intergenic
GGATAATTGGTATTAGAAAT+GGG 0.358178 4_1:-81978470 None:intergenic
GATGAGAGATTGAGATAGAT+AGG 0.360209 4_1:-81978397 None:intergenic
GGCCTAGCAAGGCTAGAATT+TGG 0.367401 4_1:-81980183 None:intergenic
ATGAAGAGATGAGGGAAATA+TGG 0.371552 4_1:+81980454 Msa0577640:three_prime_UTR
GTGGGTTATTGGATTGGTTA+TGG 0.372418 4_1:+81979071 Msa0577640:CDS
CCATCTGATCCGAAATTTGT+TGG 0.374348 4_1:-81980848 None:intergenic
TGGATAATTGGTATTAGAAA+TGG 0.374452 4_1:-81978471 None:intergenic
CATCTCATGTCAATGCTTCA+AGG 0.387378 4_1:+81978416 Msa0577640:five_prime_UTR
GCATGTCTCTAATAAGATAT+TGG 0.394270 4_1:+81980299 Msa0577640:three_prime_UTR
ATATGTATATATGACATGTT+TGG 0.401400 4_1:-81980776 None:intergenic
CGCTTTGTTGAATATTGAAA+TGG 0.401439 4_1:-81978621 None:intergenic
GGCGTTGAGCAGTACGATAT+TGG 0.404711 4_1:+81980475 Msa0577640:three_prime_UTR
AACAAGAAATAACTATTTCT+GGG 0.409264 4_1:-81981041 None:intergenic
GGATTTGCGTCGTTTGTTCT+CGG 0.413462 4_1:+81978985 Msa0577640:CDS
GAGAGGGAAAAGGGTATAAA+GGG 0.413563 4_1:-81978559 None:intergenic
TGAATCAAGGACAAACTAAC+TGG 0.414033 4_1:-81980204 None:intergenic
GATTTGCGTCGTTTGTTCTC+GGG 0.415303 4_1:+81978986 Msa0577640:CDS
GTACTGGATCCAACAAATTT+CGG 0.418624 4_1:+81980839 Msa0577640:three_prime_UTR
AAAATTGCAACTGTTGTTTG+TGG 0.421297 4_1:+81980086 Msa0577640:three_prime_UTR
GTCGTTTGTTCTCGGGATTT+CGG 0.429794 4_1:+81978993 Msa0577640:CDS
GATCGGTTACTTTCGTGATT+GGG 0.431361 4_1:+81979034 Msa0577640:CDS
CTCGAAGAACATGGATGTAC+TGG 0.440300 4_1:+81980823 Msa0577640:three_prime_UTR
AAGATTGAAAGAGAGGGAAA+AGG 0.443660 4_1:-81978569 None:intergenic
TAGTTATTTCTTGTTTAGAA+AGG 0.447898 4_1:+81981049 Msa0577640:three_prime_UTR
ATATGACATGTTTGGAAATG+AGG 0.454739 4_1:-81980768 None:intergenic
CTTGATTCATAACCCAGATA+AGG 0.457849 4_1:+81980218 Msa0577640:three_prime_UTR
TTTCTGGGAACAAAGTGGTT+TGG 0.463254 4_1:-81981026 None:intergenic
AGATTGAAAGAGAGGGAAAA+GGG 0.465137 4_1:-81978568 None:intergenic
GTTACCATAATTTGTGGGCA+TGG 0.468551 4_1:+81980588 Msa0577640:three_prime_UTR
TATAAGTTACCATAATTTGT+GGG 0.469366 4_1:+81980583 Msa0577640:three_prime_UTR
TTGAGACGTTCGTCGGCGGC+AGG 0.478548 4_1:+81978946 Msa0577640:CDS
CTAGAATTTGGCTACTCTCT+AGG 0.481600 4_1:-81980171 None:intergenic
ATATAAGTTACCATAATTTG+TGG 0.483320 4_1:+81980582 Msa0577640:three_prime_UTR
GTCTTTGAGTGGGAGGAGCT+GGG 0.483371 4_1:+81981001 Msa0577640:three_prime_UTR
TGCTTCAAATATGAGTTATG+TGG 0.499574 4_1:-81980536 None:intergenic
GGCTTTGATGCTGTGTGGAT+CGG 0.501281 4_1:+81979017 Msa0577640:CDS
ATAGCATCATTCTGTTATGT+TGG 0.502512 4_1:+81980377 Msa0577640:three_prime_UTR
CTTATCTGGGTTATGAATCA+AGG 0.503210 4_1:-81980217 None:intergenic
AAAGACGAAGATTGAAAGAG+AGG 0.505181 4_1:-81978576 None:intergenic
TTGAGAATTCCATGCTTGAC+AGG 0.510579 4_1:+81979884 Msa0577640:CDS
GATGCCGTGGGTTATTGGAT+TGG 0.512161 4_1:+81979065 Msa0577640:CDS
CTCAACACCGACGGATTCAC+CGG 0.514030 4_1:-81978928 None:intergenic
AAGACGAAGATTGAAAGAGA+GGG 0.514704 4_1:-81978575 None:intergenic
GACGGATTCACCGGCACGAT+CGG 0.516430 4_1:-81978919 None:intergenic
CAACCATGTCAAAGCTCGAC+AGG 0.517655 4_1:+81981083 Msa0577640:three_prime_UTR
CCAACAAATTTCGGATCAGA+TGG 0.520077 4_1:+81980848 Msa0577640:three_prime_UTR
CAAAATAAACAGCAGAAATG+AGG 0.523916 4_1:-81980328 None:intergenic
TTCTAAAGCACTAAGAATTG+AGG 0.524292 4_1:+81980642 Msa0577640:three_prime_UTR
ATTCCATGCTTGACAGGTCT+AGG 0.525166 4_1:+81979890 Msa0577640:CDS
GATCGTGCCGGTGAATCCGT+CGG 0.526152 4_1:+81978921 Msa0577640:CDS
TTGATTCATAACCCAGATAA+GGG 0.528184 4_1:+81980219 Msa0577640:three_prime_UTR
TTTGACAAGAGGGGTTTGCT+TGG 0.532173 4_1:-81978528 None:intergenic
TGGCGAAGCAAAGCAAAGAA+TGG 0.536106 4_1:-81979142 None:intergenic
GTTCTATAGAGTGCATGAGC+AGG 0.538940 4_1:-81980505 None:intergenic
CACATTGCCACGTCTTTGAG+TGG 0.543568 4_1:+81980990 Msa0577640:three_prime_UTR
AAACTGGGTTACGTGAGGAA+TGG 0.550237 4_1:-81978491 None:intergenic
GATAATTGGTATTAGAAATG+GGG 0.550540 4_1:-81978469 None:intergenic
GAAATTGTTATGAAGAGATG+AGG 0.551132 4_1:+81980445 Msa0577640:three_prime_UTR
GTCGGTGTTGAGACGTTCGT+CGG 0.552778 4_1:+81978939 Msa0577640:CDS
CGGCACGATCGGAGATCGAT+CGG 0.564859 4_1:-81978908 None:intergenic
ACGCCTGTCGAGCTTTGACA+TGG 0.570004 4_1:-81981086 None:intergenic
ACATTGCCACGTCTTTGAGT+GGG 0.581057 4_1:+81980991 Msa0577640:three_prime_UTR
ACATAACTCATATTTGAAGC+AGG 0.582255 4_1:+81980538 Msa0577640:three_prime_UTR
CGATCGATCTCCGATCGTGC+CGG 0.583698 4_1:+81978909 Msa0577640:CDS
TGTTATAACAGGTATTGATG+TGG 0.584670 4_1:+81980925 Msa0577640:three_prime_UTR
GTACCTAGACCTGTCAAGCA+TGG 0.588585 4_1:-81979893 None:intergenic
TGGGTTTATGTTGATGCCGT+GGG 0.591957 4_1:+81979053 Msa0577640:CDS
CATAACCCAGATAAGGGTCA+AGG 0.592729 4_1:+81980225 Msa0577640:three_prime_UTR
TGGTGAACTGATTGATGCAT+GGG 0.593183 4_1:+81980124 Msa0577640:three_prime_UTR
AGACATGCTTCAATGAGTTG+AGG 0.594995 4_1:-81980284 None:intergenic
GATCGATCGGAATAAGATAA+CGG 0.597785 4_1:-81978895 None:intergenic
AACTATTTCTGGGAACAAAG+TGG 0.598692 4_1:-81981031 None:intergenic
TTGGGTTTATGTTGATGCCG+TGG 0.602091 4_1:+81979052 Msa0577640:CDS
ATGGTGAACTGATTGATGCA+TGG 0.603148 4_1:+81980123 Msa0577640:three_prime_UTR
ACAAACTAACTGGCCTAGCA+AGG 0.603397 4_1:-81980194 None:intergenic
AGCCAAATTCTAGCCTTGCT+AGG 0.604360 4_1:+81980181 Msa0577640:three_prime_UTR
CATATTTGAAGCAGGAATCG+TGG 0.613855 4_1:+81980546 Msa0577640:three_prime_UTR
TTGCCACGTCTTTGAGTGGG+AGG 0.614025 4_1:+81980994 Msa0577640:three_prime_UTR
ATAATTGGTATTAGAAATGG+GGG 0.617519 4_1:-81978468 None:intergenic
GTTTGTTCTCGGGATTTCGG+TGG 0.621102 4_1:+81978996 Msa0577640:CDS
GCAGGTCAGATAACTCCGGC+GGG 0.623444 4_1:+81978964 Msa0577640:CDS
AAATTGTTATGAAGAGATGA+GGG 0.626191 4_1:+81980446 Msa0577640:three_prime_UTR
GACGAACGTCTCAACACCGA+CGG 0.630029 4_1:-81978937 None:intergenic
GGTGTTGAGACGTTCGTCGG+CGG 0.630502 4_1:+81978942 Msa0577640:CDS
TCGCCACGTAAAGATTCTCC+CGG 0.632756 4_1:+81979159 Msa0577640:CDS
TCGGTGGCTTTGATGCTGTG+TGG 0.645493 4_1:+81979012 Msa0577640:CDS
GGCAGGTCAGATAACTCCGG+CGG 0.647696 4_1:+81978963 Msa0577640:CDS
GGCGGCAGGTCAGATAACTC+CGG 0.648034 4_1:+81978960 Msa0577640:CDS
TCGGAATAAGATAACGGCGT+CGG 0.648078 4_1:-81978889 None:intergenic
CACTCAAAGACGTGGCAATG+TGG 0.656986 4_1:-81980989 None:intergenic
TCATATATACATATATAGAG+AGG 0.657329 4_1:+81980785 Msa0577640:three_prime_UTR
ACAAACGACGCAAATCCCGC+CGG 0.670719 4_1:-81978979 None:intergenic
ATAACCAATCCAATAACCCA+CGG 0.672738 4_1:-81979069 None:intergenic
ATGATTTGTCTCGAAGAACA+TGG 0.674713 4_1:+81980814 Msa0577640:three_prime_UTR
TTACCGGGAGAATCTTTACG+TGG 0.685734 4_1:-81979162 None:intergenic
GCTCCTCCCACTCAAAGACG+TGG 0.694399 4_1:-81980997 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGAGATAAATTTATATGTA+TGG + chr4_1:81979337-81979356 Msa0577640:intron 15.0%
!!! GTTTATCTTCTTTAAAATTA+AGG + chr4_1:81979608-81979627 Msa0577640:intron 15.0%
!! CATATTACATGAAATAATTT+CGG - chr4_1:81980893-81980912 None:intergenic 15.0%
!!! TGCTTTTTTAGAATTTTATA+TGG + chr4_1:81980958-81980977 Msa0577640:three_prime_UTR 15.0%
!!! TTTTTAGAATTTTATATGGT+AGG + chr4_1:81980962-81980981 Msa0577640:three_prime_UTR 15.0%
!! GTTAATGAAAAAGAAAAACT+GGG - chr4_1:81978509-81978528 None:intergenic 20.0%
!! TTATGTTTCTTAGTTAGTTA+TGG + chr4_1:81979274-81979293 Msa0577640:intron 20.0%
!! TATGTTTCTTAGTTAGTTAT+GGG + chr4_1:81979275-81979294 Msa0577640:intron 20.0%
!! AAGAAGATAAACATGTATAT+CGG - chr4_1:81979600-81979619 None:intergenic 20.0%
!!! ATCTTCTTTAAAATTAAGGT+TGG + chr4_1:81979612-81979631 Msa0577640:intron 20.0%
!!! ATTGTAGCTTTCATTTTAAA+AGG + chr4_1:81979848-81979867 Msa0577640:CDS 20.0%
!!! TTGTAGCTTTCATTTTAAAA+GGG + chr4_1:81979849-81979868 Msa0577640:CDS 20.0%
!!! TTTTAAAAGGGATATTCATA+TGG + chr4_1:81979861-81979880 Msa0577640:CDS 20.0%
!!! CATACAACAAATTAGTTTTT+TGG - chr4_1:81979995-81980014 None:intergenic 20.0%
!! ATATAAGTTACCATAATTTG+TGG + chr4_1:81980582-81980601 Msa0577640:three_prime_UTR 20.0%
!!! TATAAGTTACCATAATTTGT+GGG + chr4_1:81980583-81980602 Msa0577640:three_prime_UTR 20.0%
!! AACAGATATCAATAAAACAA+AGG - chr4_1:81980701-81980720 None:intergenic 20.0%
!! ATCTGTAAATTGCTAATTAT+AGG + chr4_1:81980726-81980745 Msa0577640:intron 20.0%
!! ATATGTATATATGACATGTT+TGG - chr4_1:81980779-81980798 None:intergenic 20.0%
!! TCATATATACATATATAGAG+AGG + chr4_1:81980785-81980804 Msa0577640:three_prime_UTR 20.0%
!! GATGTTATTATTGTTATAAC+AGG + chr4_1:81980914-81980933 Msa0577640:three_prime_UTR 20.0%
!!! AACAAGAAATAACTATTTCT+GGG - chr4_1:81981044-81981063 None:intergenic 20.0%
!! AAACAAGAAATAACTATTTC+TGG - chr4_1:81981045-81981064 None:intergenic 20.0%
!!! TAGTTATTTCTTGTTTAGAA+AGG + chr4_1:81981049-81981068 Msa0577640:three_prime_UTR 20.0%
! ATAATTGGTATTAGAAATGG+GGG - chr4_1:81978471-81978490 None:intergenic 25.0%
! GATAATTGGTATTAGAAATG+GGG - chr4_1:81978472-81978491 None:intergenic 25.0%
! GGATAATTGGTATTAGAAAT+GGG - chr4_1:81978473-81978492 None:intergenic 25.0%
! TGGATAATTGGTATTAGAAA+TGG - chr4_1:81978474-81978493 None:intergenic 25.0%
! GGTTAATGAAAAAGAAAAAC+TGG - chr4_1:81978510-81978529 None:intergenic 25.0%
! AAGAAGAAAGAAGAAGAAAT+TGG - chr4_1:81978670-81978689 None:intergenic 25.0%
!! CTCATCATTTTCTTGATTTT+TGG - chr4_1:81978787-81978806 None:intergenic 25.0%
!! TGTTTCTTTTCTTTCTGTTT+TGG + chr4_1:81979113-81979132 Msa0577640:CDS 25.0%
!! GTTTCTTTTCTTTCTGTTTT+GGG + chr4_1:81979114-81979133 Msa0577640:CDS 25.0%
! ATAGATTATGATTTCTTACC+GGG - chr4_1:81979180-81979199 None:intergenic 25.0%
! CATAGATTATGATTTCTTAC+CGG - chr4_1:81979181-81979200 None:intergenic 25.0%
! ATGTTTCTTAGTTAGTTATG+GGG + chr4_1:81979276-81979295 Msa0577640:intron 25.0%
!! ATTTAAAACGATAACACACA+GGG - chr4_1:81979430-81979449 None:intergenic 25.0%
!! AATTTAAAACGATAACACAC+AGG - chr4_1:81979431-81979450 None:intergenic 25.0%
! CAGATAATTGTAGTTAATTG+CGG + chr4_1:81979478-81979497 Msa0577640:intron 25.0%
!! GAATTTTTCTGTAATCAGAA+AGG + chr4_1:81979755-81979774 Msa0577640:intron 25.0%
!! TTTTATCCGTTCATGATTTT+AGG + chr4_1:81979823-81979842 Msa0577640:intron 25.0%
! CAATAACCTAAAATCATGAA+CGG - chr4_1:81979832-81979851 None:intergenic 25.0%
! GTCTAGGTACATATTATATT+GGG + chr4_1:81979906-81979925 Msa0577640:intron 25.0%
! ATATTATATTGGGAGTAAGT+GGG + chr4_1:81979916-81979935 Msa0577640:intron 25.0%
!!! TAGTTTTTTGGTATTCATCA+AGG - chr4_1:81979983-81980002 None:intergenic 25.0%
!!! TTTATTTTGACTTGAATGTG+TGG + chr4_1:81980342-81980361 Msa0577640:three_prime_UTR 25.0%
! AAATTGTTATGAAGAGATGA+GGG + chr4_1:81980446-81980465 Msa0577640:three_prime_UTR 25.0%
! CGCTTTGTTGAATATTGAAA+TGG - chr4_1:81978624-81978643 None:intergenic 30.0%
AAGAAGAAGAAATTGGATAG+AGG - chr4_1:81978663-81978682 None:intergenic 30.0%
!! CATAATCTATGTTGCTTTCT+TGG + chr4_1:81979190-81979209 Msa0577640:intron 30.0%
TTTCTTGGTTCAAATATTGC+TGG + chr4_1:81979205-81979224 Msa0577640:intron 30.0%
! CAAATATTGCTGGTTAGAAT+TGG + chr4_1:81979215-81979234 Msa0577640:intron 30.0%
ACATTTAAGAGTAGTAGTAG+TGG + chr4_1:81979377-81979396 Msa0577640:intron 30.0%
TGAAGAGACATCAAATACAT+AGG + chr4_1:81979522-81979541 Msa0577640:intron 30.0%
AGACATCAAATACATAGGAT+GGG + chr4_1:81979527-81979546 Msa0577640:intron 30.0%
AAATTATACACTTTCTGTGG+TGG - chr4_1:81979682-81979701 None:intergenic 30.0%
TGCAAATTATACACTTTCTG+TGG - chr4_1:81979685-81979704 None:intergenic 30.0%
GGTCTAGGTACATATTATAT+TGG + chr4_1:81979905-81979924 Msa0577640:intron 30.0%
CATATTATATTGGGAGTAAG+TGG + chr4_1:81979915-81979934 Msa0577640:intron 30.0%
TATTATATTGGGAGTAAGTG+GGG + chr4_1:81979917-81979936 Msa0577640:intron 30.0%
!!! TGCTTTTTTCAGTCAAAGAT+TGG + chr4_1:81980013-81980032 Msa0577640:intron 30.0%
!!! GCTTTTTTCAGTCAAAGATT+GGG + chr4_1:81980014-81980033 Msa0577640:intron 30.0%
! AAAATTGCAACTGTTGTTTG+TGG + chr4_1:81980086-81980105 Msa0577640:three_prime_UTR 30.0%
TGTGGAGTTTGACATTATTA+TGG + chr4_1:81980104-81980123 Msa0577640:three_prime_UTR 30.0%
TTGATTCATAACCCAGATAA+GGG + chr4_1:81980219-81980238 Msa0577640:three_prime_UTR 30.0%
GCATGTCTCTAATAAGATAT+TGG + chr4_1:81980299-81980318 Msa0577640:three_prime_UTR 30.0%
CAAAATAAACAGCAGAAATG+AGG - chr4_1:81980331-81980350 None:intergenic 30.0%
ATAGCATCATTCTGTTATGT+TGG + chr4_1:81980377-81980396 Msa0577640:three_prime_UTR 30.0%
AAAAATCATGTAATGGTGTC+AGG - chr4_1:81980411-81980430 None:intergenic 30.0%
CAACAGCAAAAATCATGTAA+TGG - chr4_1:81980418-81980437 None:intergenic 30.0%
GAAATTGTTATGAAGAGATG+AGG + chr4_1:81980445-81980464 Msa0577640:three_prime_UTR 30.0%
TGCTTCAAATATGAGTTATG+TGG - chr4_1:81980539-81980558 None:intergenic 30.0%
! ACATAACTCATATTTGAAGC+AGG + chr4_1:81980538-81980557 Msa0577640:three_prime_UTR 30.0%
!! TTCTAAAGCACTAAGAATTG+AGG + chr4_1:81980642-81980661 Msa0577640:three_prime_UTR 30.0%
TGTTTGGAAATGAGGAAAAA+AGG - chr4_1:81980763-81980782 None:intergenic 30.0%
ATATGACATGTTTGGAAATG+AGG - chr4_1:81980771-81980790 None:intergenic 30.0%
!! TGTTATAACAGGTATTGATG+TGG + chr4_1:81980925-81980944 Msa0577640:three_prime_UTR 30.0%
TTACGTGAGGAATGGATAAT+TGG - chr4_1:81978486-81978505 None:intergenic 35.0%
!! GAAAAAGTGTTTGACAAGAG+GGG - chr4_1:81978540-81978559 None:intergenic 35.0%
!! GGAAAAAGTGTTTGACAAGA+GGG - chr4_1:81978541-81978560 None:intergenic 35.0%
AGATTGAAAGAGAGGGAAAA+GGG - chr4_1:81978571-81978590 None:intergenic 35.0%
AAGATTGAAAGAGAGGGAAA+AGG - chr4_1:81978572-81978591 None:intergenic 35.0%
AAGACGAAGATTGAAAGAGA+GGG - chr4_1:81978578-81978597 None:intergenic 35.0%
AAAGACGAAGATTGAAAGAG+AGG - chr4_1:81978579-81978598 None:intergenic 35.0%
GATCGATCGGAATAAGATAA+CGG - chr4_1:81978898-81978917 None:intergenic 35.0%
ATAACCAATCCAATAACCCA+CGG - chr4_1:81979072-81979091 None:intergenic 35.0%
! GTTATGGTGTTTTATGTTGC+TGG + chr4_1:81979087-81979106 Msa0577640:CDS 35.0%
CTGCATTAACAATGATCACT+TGG - chr4_1:81979461-81979480 None:intergenic 35.0%
ATGTCTCTTCAACCACATTA+CGG - chr4_1:81979513-81979532 None:intergenic 35.0%
GAGACATCAAATACATAGGA+TGG + chr4_1:81979526-81979545 Msa0577640:intron 35.0%
! TAATTTGCAACACCCATATC+AGG + chr4_1:81979696-81979715 Msa0577640:intron 35.0%
AATTTGCAACACCCATATCA+GGG + chr4_1:81979697-81979716 Msa0577640:intron 35.0%
ATCTAAGCTATCCCTGATAT+GGG - chr4_1:81979711-81979730 None:intergenic 35.0%
TATCTAAGCTATCCCTGATA+TGG - chr4_1:81979712-81979731 None:intergenic 35.0%
AGTAAGTGGGGTTAAGTTTA+TGG + chr4_1:81979929-81979948 Msa0577640:intron 35.0%
GTAAGTGGGGTTAAGTTTAT+GGG + chr4_1:81979930-81979949 Msa0577640:intron 35.0%
TGAATCAAGGACAAACTAAC+TGG - chr4_1:81980207-81980226 None:intergenic 35.0%
CTTATCTGGGTTATGAATCA+AGG - chr4_1:81980220-81980239 None:intergenic 35.0%
CTTGATTCATAACCCAGATA+AGG + chr4_1:81980218-81980237 Msa0577640:three_prime_UTR 35.0%
! TTTTACCTTGACCCTTATCT+GGG - chr4_1:81980233-81980252 None:intergenic 35.0%
ATGAAGAGATGAGGGAAATA+TGG + chr4_1:81980454-81980473 Msa0577640:three_prime_UTR 35.0%
GATAACAAAAGAAAGAAGCC+AGG - chr4_1:81980679-81980698 None:intergenic 35.0%
! ATGATTTGTCTCGAAGAACA+TGG + chr4_1:81980814-81980833 Msa0577640:three_prime_UTR 35.0%
GTACTGGATCCAACAAATTT+CGG + chr4_1:81980839-81980858 Msa0577640:three_prime_UTR 35.0%
!! AACTATTTCTGGGAACAAAG+TGG - chr4_1:81981034-81981053 None:intergenic 35.0%
CATCTCATGTCAATGCTTCA+AGG + chr4_1:81978416-81978435 Msa0577640:five_prime_UTR 40.0%
AAGAAAAACTGGGTTACGTG+AGG - chr4_1:81978499-81978518 None:intergenic 40.0%
!! GGGAAAAAGTGTTTGACAAG+AGG - chr4_1:81978542-81978561 None:intergenic 40.0%
! GAGAGGGAAAAGGGTATAAA+GGG - chr4_1:81978562-81978581 None:intergenic 40.0%
! AGAGAGGGAAAAGGGTATAA+AGG - chr4_1:81978563-81978582 None:intergenic 40.0%
! GATCGGTTACTTTCGTGATT+GGG + chr4_1:81979034-81979053 Msa0577640:CDS 40.0%
!! GTGGGTTATTGGATTGGTTA+TGG + chr4_1:81979071-81979090 Msa0577640:CDS 40.0%
TTGTAGTTAATTGCGGCTGA+TGG + chr4_1:81979485-81979504 Msa0577640:intron 40.0%
TGTAGTTAATTGCGGCTGAT+GGG + chr4_1:81979486-81979505 Msa0577640:intron 40.0%
TTGAGAATTCCATGCTTGAC+AGG + chr4_1:81979884-81979903 Msa0577640:CDS 40.0%
ATGGTGAACTGATTGATGCA+TGG + chr4_1:81980123-81980142 Msa0577640:three_prime_UTR 40.0%
TGGTGAACTGATTGATGCAT+GGG + chr4_1:81980124-81980143 Msa0577640:three_prime_UTR 40.0%
CTAGAATTTGGCTACTCTCT+AGG - chr4_1:81980174-81980193 None:intergenic 40.0%
! CTTTTACCTTGACCCTTATC+TGG - chr4_1:81980234-81980253 None:intergenic 40.0%
AGACATGCTTCAATGAGTTG+AGG - chr4_1:81980287-81980306 None:intergenic 40.0%
!!! TGATTTTTGCTGTTGCAGAG+TGG + chr4_1:81980423-81980442 Msa0577640:three_prime_UTR 40.0%
! CATATTTGAAGCAGGAATCG+TGG + chr4_1:81980546-81980565 Msa0577640:three_prime_UTR 40.0%
! GTTACCATAATTTGTGGGCA+TGG + chr4_1:81980588-81980607 Msa0577640:three_prime_UTR 40.0%
AACAAAAGAAAGAAGCCAGG+CGG - chr4_1:81980676-81980695 None:intergenic 40.0%
CCATCTGATCCGAAATTTGT+TGG - chr4_1:81980851-81980870 None:intergenic 40.0%
CCAACAAATTTCGGATCAGA+TGG + chr4_1:81980848-81980867 Msa0577640:three_prime_UTR 40.0%
! TTTCTGGGAACAAAGTGGTT+TGG - chr4_1:81981029-81981048 None:intergenic 40.0%
AAACTGGGTTACGTGAGGAA+TGG - chr4_1:81978494-81978513 None:intergenic 45.0%
! TTTGACAAGAGGGGTTTGCT+TGG - chr4_1:81978531-81978550 None:intergenic 45.0%
! TCGGAATAAGATAACGGCGT+CGG - chr4_1:81978892-81978911 None:intergenic 45.0%
GGATTTGCGTCGTTTGTTCT+CGG + chr4_1:81978985-81979004 Msa0577640:CDS 45.0%
GATTTGCGTCGTTTGTTCTC+GGG + chr4_1:81978986-81979005 Msa0577640:CDS 45.0%
GTCGTTTGTTCTCGGGATTT+CGG + chr4_1:81978993-81979012 Msa0577640:CDS 45.0%
! GGATCGGTTACTTTCGTGAT+TGG + chr4_1:81979033-81979052 Msa0577640:CDS 45.0%
TTGGGTTTATGTTGATGCCG+TGG + chr4_1:81979052-81979071 Msa0577640:CDS 45.0%
TGGGTTTATGTTGATGCCGT+GGG + chr4_1:81979053-81979072 Msa0577640:CDS 45.0%
! ATGTTGATGCCGTGGGTTAT+TGG + chr4_1:81979060-81979079 Msa0577640:CDS 45.0%
TGGCGAAGCAAAGCAAAGAA+TGG - chr4_1:81979145-81979164 None:intergenic 45.0%
TTACCGGGAGAATCTTTACG+TGG - chr4_1:81979165-81979184 None:intergenic 45.0%
GTAGTTAATTGCGGCTGATG+GGG + chr4_1:81979487-81979506 Msa0577640:intron 45.0%
ATTCCATGCTTGACAGGTCT+AGG + chr4_1:81979890-81979909 Msa0577640:CDS 45.0%
!! AGCCAAATTCTAGCCTTGCT+AGG + chr4_1:81980181-81980200 Msa0577640:three_prime_UTR 45.0%
! ACAAACTAACTGGCCTAGCA+AGG - chr4_1:81980197-81980216 None:intergenic 45.0%
CATAACCCAGATAAGGGTCA+AGG + chr4_1:81980225-81980244 Msa0577640:three_prime_UTR 45.0%
GTTCTATAGAGTGCATGAGC+AGG - chr4_1:81980508-81980527 None:intergenic 45.0%
GAAGCCATGCCCACAAATTA+TGG - chr4_1:81980595-81980614 None:intergenic 45.0%
ACAAAAGAAAGAAGCCAGGC+GGG - chr4_1:81980675-81980694 None:intergenic 45.0%
CTCGAAGAACATGGATGTAC+TGG + chr4_1:81980823-81980842 Msa0577640:three_prime_UTR 45.0%
ACATTGCCACGTCTTTGAGT+GGG + chr4_1:81980991-81981010 Msa0577640:three_prime_UTR 45.0%
! TGGTTGACGACGACGTTTTG+AGG - chr4_1:81978604-81978623 None:intergenic 50.0%
GTTTGTTCTCGGGATTTCGG+TGG + chr4_1:81978996-81979015 Msa0577640:CDS 50.0%
! GGCTTTGATGCTGTGTGGAT+CGG + chr4_1:81979017-81979036 Msa0577640:CDS 50.0%
! GATGCCGTGGGTTATTGGAT+TGG + chr4_1:81979065-81979084 Msa0577640:CDS 50.0%
TCGCCACGTAAAGATTCTCC+CGG + chr4_1:81979159-81979178 Msa0577640:CDS 50.0%
GTACCTAGACCTGTCAAGCA+TGG - chr4_1:81979896-81979915 None:intergenic 50.0%
GGCCTAGCAAGGCTAGAATT+TGG - chr4_1:81980186-81980205 None:intergenic 50.0%
! GGCGTTGAGCAGTACGATAT+TGG + chr4_1:81980475-81980494 Msa0577640:three_prime_UTR 50.0%
CACTCAAAGACGTGGCAATG+TGG - chr4_1:81980992-81981011 None:intergenic 50.0%
CACATTGCCACGTCTTTGAG+TGG + chr4_1:81980990-81981009 Msa0577640:three_prime_UTR 50.0%
! CTCAACACCGACGGATTCAC+CGG - chr4_1:81978931-81978950 None:intergenic 55.0%
GACGAACGTCTCAACACCGA+CGG - chr4_1:81978940-81978959 None:intergenic 55.0%
!! GTCGGTGTTGAGACGTTCGT+CGG + chr4_1:81978939-81978958 Msa0577640:CDS 55.0%
ACAAACGACGCAAATCCCGC+CGG - chr4_1:81978982-81979001 None:intergenic 55.0%
!! TCGGTGGCTTTGATGCTGTG+TGG + chr4_1:81979012-81979031 Msa0577640:CDS 55.0%
TTGCCACGTCTTTGAGTGGG+AGG + chr4_1:81980994-81981013 Msa0577640:three_prime_UTR 55.0%
GTCTTTGAGTGGGAGGAGCT+GGG + chr4_1:81981001-81981020 Msa0577640:three_prime_UTR 55.0%
CGGCACGATCGGAGATCGAT+CGG - chr4_1:81978911-81978930 None:intergenic 60.0%
CGATCGATCTCCGATCGTGC+CGG + chr4_1:81978909-81978928 Msa0577640:CDS 60.0%
! GACGGATTCACCGGCACGAT+CGG - chr4_1:81978922-81978941 None:intergenic 60.0%
! GATCGTGCCGGTGAATCCGT+CGG + chr4_1:81978921-81978940 Msa0577640:CDS 60.0%
! GGTGTTGAGACGTTCGTCGG+CGG + chr4_1:81978942-81978961 Msa0577640:CDS 60.0%
GGCGGCAGGTCAGATAACTC+CGG + chr4_1:81978960-81978979 Msa0577640:CDS 60.0%
GGCAGGTCAGATAACTCCGG+CGG + chr4_1:81978963-81978982 Msa0577640:CDS 60.0%
GCAGGTCAGATAACTCCGGC+GGG + chr4_1:81978964-81978983 Msa0577640:CDS 60.0%
ATTGAGGAGTCGTTCCCGCC+TGG + chr4_1:81980658-81980677 Msa0577640:intron 60.0%
GCTCCTCCCACTCAAAGACG+TGG - chr4_1:81981000-81981019 None:intergenic 60.0%
CGTCTTTGAGTGGGAGGAGC+TGG + chr4_1:81981000-81981019 Msa0577640:three_prime_UTR 60.0%
TTGAGACGTTCGTCGGCGGC+AGG + chr4_1:81978946-81978965 Msa0577640:CDS 65.0%
CGGCTGATGGGGCCGTAATG+TGG + chr4_1:81979498-81979517 Msa0577640:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 81978400 81981102 81978400 ID=Msa0577640;Name=Msa0577640
chr4_1 mRNA 81978400 81981102 81978400 ID=Msa0577640-mRNA-1;Parent=Msa0577640;Name=Msa0577640-mRNA-1;_AED=0.12;_eAED=0.12;_QI=283|0.6|0.83|0.83|0.4|0.16|6|971|157
chr4_1 exon 81978400 81978437 81978400 ID=Msa0577640-mRNA-1:exon:29571;Parent=Msa0577640-mRNA-1
chr4_1 exon 81978461 81978650 81978461 ID=Msa0577640-mRNA-1:exon:29572;Parent=Msa0577640-mRNA-1
chr4_1 exon 81978744 81979180 81978744 ID=Msa0577640-mRNA-1:exon:29573;Parent=Msa0577640-mRNA-1
chr4_1 exon 81979845 81979911 81979845 ID=Msa0577640-mRNA-1:exon:29574;Parent=Msa0577640-mRNA-1
chr4_1 exon 81980025 81980665 81980025 ID=Msa0577640-mRNA-1:exon:29575;Parent=Msa0577640-mRNA-1
chr4_1 exon 81980748 81981102 81980748 ID=Msa0577640-mRNA-1:exon:29576;Parent=Msa0577640-mRNA-1
chr4_1 five_prime_UTR 81978400 81978437 81978400 ID=Msa0577640-mRNA-1:five_prime_utr;Parent=Msa0577640-mRNA-1
chr4_1 five_prime_UTR 81978461 81978650 81978461 ID=Msa0577640-mRNA-1:five_prime_utr;Parent=Msa0577640-mRNA-1
chr4_1 five_prime_UTR 81978744 81978798 81978744 ID=Msa0577640-mRNA-1:five_prime_utr;Parent=Msa0577640-mRNA-1
chr4_1 CDS 81978799 81979180 81978799 ID=Msa0577640-mRNA-1:cds;Parent=Msa0577640-mRNA-1
chr4_1 CDS 81979845 81979911 81979845 ID=Msa0577640-mRNA-1:cds;Parent=Msa0577640-mRNA-1
chr4_1 CDS 81980025 81980049 81980025 ID=Msa0577640-mRNA-1:cds;Parent=Msa0577640-mRNA-1
chr4_1 three_prime_UTR 81980050 81980665 81980050 ID=Msa0577640-mRNA-1:three_prime_utr;Parent=Msa0577640-mRNA-1
chr4_1 three_prime_UTR 81980748 81981102 81980748 ID=Msa0577640-mRNA-1:three_prime_utr;Parent=Msa0577640-mRNA-1
Gene Sequence

>Msa0577640

ATGATGAGAAGAAATCAAGATCAAGAACAACAATCAAAGATGATTTGCGAATTATCATCTCTACTTTTCAATCTTCTTCTACAATCTTCACCGACGCCGTTATCTTATTCCGATCGATCTCCGATCGTGCCGGTGAATCCGTCGGTGTTGAGACGTTCGTCGGCGGCAGGTCAGATAACTCCGGCGGGATTTGCGTCGTTTGTTCTCGGGATTTCGGTGGCTTTGATGCTGTGTGGATCGGTTACTTTCGTGATTGGGTTTATGTTGATGCCGTGGGTTATTGGATTGGTTATGGTGTTTTATGTTGCTGGTGTTGTTTCTTTTCTTTCTGTTTTGGGTCGCTCCATTCTTTGCTTTGCTTCGCCACGTAAAGATTCTCCCGGTTATTGTAGCTTTCATTTTAAAAGGGATATTCATATGGTTGAGAATTCCATGCTTGACAGGTCTAGTCAAAGATTGGGTCTGCAATATTAG
Protein sequence

>Msa0577640

MMRRNQDQEQQSKMICELSSLLFNLLLQSSPTPLSYSDRSPIVPVNPSVLRRSSAAGQITPAGFASFVLGISVALMLCGSVTFVIGFMLMPWVIGLVMVFYVAGVVSFLSVLGRSILCFASPRKDSPGYCSFHFKRDIHMVENSMLDRSSQRLGLQY*