AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0577730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0577730 MtrunA17_Chr8g0377531 93.798 129 8 0 1 129 82 210 1.81e-83 244
Msa0577730 MtrunA17_Chr3g0122261 54.815 135 47 4 1 129 1 127 7.77e-42 136
Msa0577730 MtrunA17_Chr5g0434711 54.054 74 27 2 17 90 5 71 1.58e-16 70.1
Msa0577730 MtrunA17_Chr7g0224931 42.308 130 60 3 1 117 21 148 4.40e-16 71.2
Msa0577730 MtrunA17_Chr7g0224971 42.308 130 60 3 1 117 21 148 4.40e-16 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0577730 AT1G27290.2 54.762 126 48 4 1 119 1 124 8.33e-37 124
Msa0577730 AT1G27290.1 54.762 126 48 4 1 119 1 124 8.33e-37 124

Find 106 sgRNAs with CRISPR-Local

Find 179 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACAAGAAATAACTATTTC+TGG 0.134731 4_1:-82051852 None:intergenic
GAAGCCATGCCCACAAATTA+TGG 0.284897 4_1:-82051402 None:intergenic
TGTGGAGTTTGACATTATTA+TGG 0.287210 4_1:+82050914 Msa0577730:three_prime_UTR
AGAGAGGGAAAAGGGTATAA+AGG 0.293809 4_1:-82049370 None:intergenic
GATGTTATTATTGTTATAAC+AGG 0.298413 4_1:+82051724 Msa0577730:three_prime_UTR
GGATCGGTTACTTTCGTGAT+TGG 0.305883 4_1:+82049843 Msa0577730:CDS
CGTCTTTGAGTGGGAGGAGC+TGG 0.317023 4_1:+82051810 Msa0577730:three_prime_UTR
ATGTTGATGCCGTGGGTTAT+TGG 0.333475 4_1:+82049870 Msa0577730:CDS
CATATTACATGAAATAATTT+CGG 0.334699 4_1:-82051700 None:intergenic
TTACGTGAGGAATGGATAAT+TGG 0.347004 4_1:-82049293 None:intergenic
GGATAATTGGTATTAGAAAT+GGG 0.358178 4_1:-82049280 None:intergenic
GATGAGAGATTGAGATAGAT+AGG 0.360209 4_1:-82049207 None:intergenic
GGCCTAGCAAGGCTAGAATT+TGG 0.367401 4_1:-82050993 None:intergenic
ATGAAGAGATGAGGGAAATA+TGG 0.371552 4_1:+82051264 Msa0577730:three_prime_UTR
GTGGGTTATTGGATTGGTTA+TGG 0.372418 4_1:+82049881 Msa0577730:CDS
CCATCTGATCCGAAATTTGT+TGG 0.374348 4_1:-82051658 None:intergenic
TGGATAATTGGTATTAGAAA+TGG 0.374452 4_1:-82049281 None:intergenic
CATCTCATGTCAATGCTTCA+AGG 0.387378 4_1:+82049226 Msa0577730:five_prime_UTR
GCATGTCTCTAATAAGATAT+TGG 0.394270 4_1:+82051109 Msa0577730:three_prime_UTR
ATATGTATATATGACATGTT+TGG 0.401400 4_1:-82051586 None:intergenic
CGCTTTGTTGAATATTGAAA+TGG 0.401439 4_1:-82049431 None:intergenic
GGCGTTGAGCAGTACGATAT+TGG 0.404711 4_1:+82051285 Msa0577730:three_prime_UTR
AACAAGAAATAACTATTTCT+GGG 0.409264 4_1:-82051851 None:intergenic
GGATTTGCGTCGTTTGTTCT+CGG 0.413462 4_1:+82049795 Msa0577730:CDS
GAGAGGGAAAAGGGTATAAA+GGG 0.413563 4_1:-82049369 None:intergenic
TGAATCAAGGACAAACTAAC+TGG 0.414033 4_1:-82051014 None:intergenic
GATTTGCGTCGTTTGTTCTC+GGG 0.415303 4_1:+82049796 Msa0577730:CDS
GTACTGGATCCAACAAATTT+CGG 0.418624 4_1:+82051649 Msa0577730:three_prime_UTR
AAAATTGCAACTGTTGTTTG+TGG 0.421297 4_1:+82050896 Msa0577730:three_prime_UTR
GTCGTTTGTTCTCGGGATTT+CGG 0.429794 4_1:+82049803 Msa0577730:CDS
GATCGGTTACTTTCGTGATT+GGG 0.431361 4_1:+82049844 Msa0577730:CDS
CTCGAAGAACATGGATGTAC+TGG 0.440300 4_1:+82051633 Msa0577730:three_prime_UTR
AAGATTGAAAGAGAGGGAAA+AGG 0.443660 4_1:-82049379 None:intergenic
TAGTTATTTCTTGTTTAGAA+AGG 0.447898 4_1:+82051859 Msa0577730:three_prime_UTR
ATATGACATGTTTGGAAATG+AGG 0.454739 4_1:-82051578 None:intergenic
CTTGATTCATAACCCAGATA+AGG 0.457849 4_1:+82051028 Msa0577730:three_prime_UTR
TTTCTGGGAACAAAGTGGTT+TGG 0.463254 4_1:-82051836 None:intergenic
AGATTGAAAGAGAGGGAAAA+GGG 0.465137 4_1:-82049378 None:intergenic
GTTACCATAATTTGTGGGCA+TGG 0.468551 4_1:+82051398 Msa0577730:three_prime_UTR
TATAAGTTACCATAATTTGT+GGG 0.469366 4_1:+82051393 Msa0577730:three_prime_UTR
TTGAGACGTTCGTCGGCGGC+AGG 0.478548 4_1:+82049756 Msa0577730:CDS
CTAGAATTTGGCTACTCTCT+AGG 0.481600 4_1:-82050981 None:intergenic
ATATAAGTTACCATAATTTG+TGG 0.483320 4_1:+82051392 Msa0577730:three_prime_UTR
GTCTTTGAGTGGGAGGAGCT+GGG 0.483371 4_1:+82051811 Msa0577730:three_prime_UTR
TGCTTCAAATATGAGTTATG+TGG 0.499574 4_1:-82051346 None:intergenic
GGCTTTGATGCTGTGTGGAT+CGG 0.501281 4_1:+82049827 Msa0577730:CDS
ATAGCATCATTCTGTTATGT+TGG 0.502512 4_1:+82051187 Msa0577730:three_prime_UTR
CTTATCTGGGTTATGAATCA+AGG 0.503210 4_1:-82051027 None:intergenic
AAAGACGAAGATTGAAAGAG+AGG 0.505181 4_1:-82049386 None:intergenic
TTGAGAATTCCATGCTTGAC+AGG 0.510579 4_1:+82050694 Msa0577730:CDS
GATGCCGTGGGTTATTGGAT+TGG 0.512161 4_1:+82049875 Msa0577730:CDS
CTCAACACCGACGGATTCAC+CGG 0.514030 4_1:-82049738 None:intergenic
AAGACGAAGATTGAAAGAGA+GGG 0.514704 4_1:-82049385 None:intergenic
GACGGATTCACCGGCACGAT+CGG 0.516430 4_1:-82049729 None:intergenic
CAACCATGTCAAAGCTCGAC+AGG 0.517655 4_1:+82051893 Msa0577730:three_prime_UTR
CCAACAAATTTCGGATCAGA+TGG 0.520077 4_1:+82051658 Msa0577730:three_prime_UTR
CAAAATAAACAGCAGAAATG+AGG 0.523916 4_1:-82051138 None:intergenic
TTCTAAAGCACTAAGAATTG+AGG 0.524292 4_1:+82051452 Msa0577730:three_prime_UTR
ATTCCATGCTTGACAGGTCT+AGG 0.525166 4_1:+82050700 Msa0577730:CDS
GATCGTGCCGGTGAATCCGT+CGG 0.526152 4_1:+82049731 Msa0577730:CDS
TTGATTCATAACCCAGATAA+GGG 0.528184 4_1:+82051029 Msa0577730:three_prime_UTR
TTTGACAAGAGGGGTTTGCT+TGG 0.532173 4_1:-82049338 None:intergenic
TGGCGAAGCAAAGCAAAGAA+TGG 0.536106 4_1:-82049952 None:intergenic
GTTCTATAGAGTGCATGAGC+AGG 0.538940 4_1:-82051315 None:intergenic
CACATTGCCACGTCTTTGAG+TGG 0.543568 4_1:+82051800 Msa0577730:three_prime_UTR
AAACTGGGTTACGTGAGGAA+TGG 0.550237 4_1:-82049301 None:intergenic
GATAATTGGTATTAGAAATG+GGG 0.550540 4_1:-82049279 None:intergenic
GAAATTGTTATGAAGAGATG+AGG 0.551132 4_1:+82051255 Msa0577730:three_prime_UTR
GTCGGTGTTGAGACGTTCGT+CGG 0.552778 4_1:+82049749 Msa0577730:CDS
CGGCACGATCGGAGATCGAT+CGG 0.564859 4_1:-82049718 None:intergenic
ACGCCTGTCGAGCTTTGACA+TGG 0.570004 4_1:-82051896 None:intergenic
ACATTGCCACGTCTTTGAGT+GGG 0.581057 4_1:+82051801 Msa0577730:three_prime_UTR
ACATAACTCATATTTGAAGC+AGG 0.582255 4_1:+82051348 Msa0577730:three_prime_UTR
CGATCGATCTCCGATCGTGC+CGG 0.583698 4_1:+82049719 Msa0577730:CDS
TGTTATAACAGGTATTGATG+TGG 0.584670 4_1:+82051735 Msa0577730:three_prime_UTR
GTACCTAGACCTGTCAAGCA+TGG 0.588585 4_1:-82050703 None:intergenic
TGGGTTTATGTTGATGCCGT+GGG 0.591957 4_1:+82049863 Msa0577730:CDS
CATAACCCAGATAAGGGTCA+AGG 0.592729 4_1:+82051035 Msa0577730:three_prime_UTR
TGGTGAACTGATTGATGCAT+GGG 0.593183 4_1:+82050934 Msa0577730:three_prime_UTR
AGACATGCTTCAATGAGTTG+AGG 0.594995 4_1:-82051094 None:intergenic
GATCGATCGGAATAAGATAA+CGG 0.597785 4_1:-82049705 None:intergenic
AACTATTTCTGGGAACAAAG+TGG 0.598692 4_1:-82051841 None:intergenic
TTGGGTTTATGTTGATGCCG+TGG 0.602091 4_1:+82049862 Msa0577730:CDS
ATGGTGAACTGATTGATGCA+TGG 0.603148 4_1:+82050933 Msa0577730:three_prime_UTR
ACAAACTAACTGGCCTAGCA+AGG 0.603397 4_1:-82051004 None:intergenic
AGCCAAATTCTAGCCTTGCT+AGG 0.604360 4_1:+82050991 Msa0577730:three_prime_UTR
CATATTTGAAGCAGGAATCG+TGG 0.613855 4_1:+82051356 Msa0577730:three_prime_UTR
TTGCCACGTCTTTGAGTGGG+AGG 0.614025 4_1:+82051804 Msa0577730:three_prime_UTR
ATAATTGGTATTAGAAATGG+GGG 0.617519 4_1:-82049278 None:intergenic
GTTTGTTCTCGGGATTTCGG+TGG 0.621102 4_1:+82049806 Msa0577730:CDS
GCAGGTCAGATAACTCCGGC+GGG 0.623444 4_1:+82049774 Msa0577730:CDS
AAATTGTTATGAAGAGATGA+GGG 0.626191 4_1:+82051256 Msa0577730:three_prime_UTR
GACGAACGTCTCAACACCGA+CGG 0.630029 4_1:-82049747 None:intergenic
GGTGTTGAGACGTTCGTCGG+CGG 0.630502 4_1:+82049752 Msa0577730:CDS
TCGCCACGTAAAGATTCTCC+CGG 0.632756 4_1:+82049969 Msa0577730:CDS
TCGGTGGCTTTGATGCTGTG+TGG 0.645493 4_1:+82049822 Msa0577730:CDS
GGCAGGTCAGATAACTCCGG+CGG 0.647696 4_1:+82049773 Msa0577730:CDS
GGCGGCAGGTCAGATAACTC+CGG 0.648034 4_1:+82049770 Msa0577730:CDS
TCGGAATAAGATAACGGCGT+CGG 0.648078 4_1:-82049699 None:intergenic
CACTCAAAGACGTGGCAATG+TGG 0.656986 4_1:-82051799 None:intergenic
TCATATATACATATATAGAG+AGG 0.657329 4_1:+82051595 Msa0577730:three_prime_UTR
ACAAACGACGCAAATCCCGC+CGG 0.670719 4_1:-82049789 None:intergenic
ATAACCAATCCAATAACCCA+CGG 0.672738 4_1:-82049879 None:intergenic
ATGATTTGTCTCGAAGAACA+TGG 0.674713 4_1:+82051624 Msa0577730:three_prime_UTR
TTACCGGGAGAATCTTTACG+TGG 0.685734 4_1:-82049972 None:intergenic
GCTCCTCCCACTCAAAGACG+TGG 0.694399 4_1:-82051807 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGAGATAAATTTATATGTA+TGG + chr4_1:82050147-82050166 Msa0577730:intron 15.0%
!!! GTTTATCTTCTTTAAAATTA+AGG + chr4_1:82050418-82050437 Msa0577730:intron 15.0%
!! CATATTACATGAAATAATTT+CGG - chr4_1:82051703-82051722 None:intergenic 15.0%
!!! TGCTTTTTTAGAATTTTATA+TGG + chr4_1:82051768-82051787 Msa0577730:three_prime_UTR 15.0%
!!! TTTTTAGAATTTTATATGGT+AGG + chr4_1:82051772-82051791 Msa0577730:three_prime_UTR 15.0%
!! GTTAATGAAAAAGAAAAACT+GGG - chr4_1:82049319-82049338 None:intergenic 20.0%
!! TTATGTTTCTTAGTTAGTTA+TGG + chr4_1:82050084-82050103 Msa0577730:intron 20.0%
!! TATGTTTCTTAGTTAGTTAT+GGG + chr4_1:82050085-82050104 Msa0577730:intron 20.0%
!! AAGAAGATAAACATGTATAT+CGG - chr4_1:82050410-82050429 None:intergenic 20.0%
!!! ATCTTCTTTAAAATTAAGGT+TGG + chr4_1:82050422-82050441 Msa0577730:intron 20.0%
!!! ATTGTAGCTTTCATTTTAAA+AGG + chr4_1:82050658-82050677 Msa0577730:CDS 20.0%
!!! TTGTAGCTTTCATTTTAAAA+GGG + chr4_1:82050659-82050678 Msa0577730:CDS 20.0%
!!! TTTTAAAAGGGATATTCATA+TGG + chr4_1:82050671-82050690 Msa0577730:CDS 20.0%
!!! CATACAACAAATTAGTTTTT+TGG - chr4_1:82050805-82050824 None:intergenic 20.0%
!! ATATAAGTTACCATAATTTG+TGG + chr4_1:82051392-82051411 Msa0577730:three_prime_UTR 20.0%
!!! TATAAGTTACCATAATTTGT+GGG + chr4_1:82051393-82051412 Msa0577730:three_prime_UTR 20.0%
!! AACAGATATCAATAAAACAA+AGG - chr4_1:82051511-82051530 None:intergenic 20.0%
!! ATCTGTAAATTGCTAATTAT+AGG + chr4_1:82051536-82051555 Msa0577730:intron 20.0%
!! ATATGTATATATGACATGTT+TGG - chr4_1:82051589-82051608 None:intergenic 20.0%
!! TCATATATACATATATAGAG+AGG + chr4_1:82051595-82051614 Msa0577730:three_prime_UTR 20.0%
!! GATGTTATTATTGTTATAAC+AGG + chr4_1:82051724-82051743 Msa0577730:three_prime_UTR 20.0%
!!! AACAAGAAATAACTATTTCT+GGG - chr4_1:82051854-82051873 None:intergenic 20.0%
!! AAACAAGAAATAACTATTTC+TGG - chr4_1:82051855-82051874 None:intergenic 20.0%
!!! TAGTTATTTCTTGTTTAGAA+AGG + chr4_1:82051859-82051878 Msa0577730:three_prime_UTR 20.0%
! ATAATTGGTATTAGAAATGG+GGG - chr4_1:82049281-82049300 None:intergenic 25.0%
! GATAATTGGTATTAGAAATG+GGG - chr4_1:82049282-82049301 None:intergenic 25.0%
! GGATAATTGGTATTAGAAAT+GGG - chr4_1:82049283-82049302 None:intergenic 25.0%
! TGGATAATTGGTATTAGAAA+TGG - chr4_1:82049284-82049303 None:intergenic 25.0%
! GGTTAATGAAAAAGAAAAAC+TGG - chr4_1:82049320-82049339 None:intergenic 25.0%
! AAGAAGAAAGAAGAAGAAAT+TGG - chr4_1:82049480-82049499 None:intergenic 25.0%
!! CTCATCATTTTCTTGATTTT+TGG - chr4_1:82049597-82049616 None:intergenic 25.0%
!! TGTTTCTTTTCTTTCTGTTT+TGG + chr4_1:82049923-82049942 Msa0577730:CDS 25.0%
!! GTTTCTTTTCTTTCTGTTTT+GGG + chr4_1:82049924-82049943 Msa0577730:CDS 25.0%
! ATAGATTATGATTTCTTACC+GGG - chr4_1:82049990-82050009 None:intergenic 25.0%
! CATAGATTATGATTTCTTAC+CGG - chr4_1:82049991-82050010 None:intergenic 25.0%
! ATGTTTCTTAGTTAGTTATG+GGG + chr4_1:82050086-82050105 Msa0577730:intron 25.0%
!! ATTTAAAACGATAACACACA+GGG - chr4_1:82050240-82050259 None:intergenic 25.0%
!! AATTTAAAACGATAACACAC+AGG - chr4_1:82050241-82050260 None:intergenic 25.0%
! CAGATAATTGTAGTTAATTG+CGG + chr4_1:82050288-82050307 Msa0577730:intron 25.0%
!! GAATTTTTCTGTAATCAGAA+AGG + chr4_1:82050565-82050584 Msa0577730:intron 25.0%
!! TTTTATCCGTTCATGATTTT+AGG + chr4_1:82050633-82050652 Msa0577730:intron 25.0%
! CAATAACCTAAAATCATGAA+CGG - chr4_1:82050642-82050661 None:intergenic 25.0%
! GTCTAGGTACATATTATATT+GGG + chr4_1:82050716-82050735 Msa0577730:intron 25.0%
! ATATTATATTGGGAGTAAGT+GGG + chr4_1:82050726-82050745 Msa0577730:intron 25.0%
!!! TAGTTTTTTGGTATTCATCA+AGG - chr4_1:82050793-82050812 None:intergenic 25.0%
!!! TTTATTTTGACTTGAATGTG+TGG + chr4_1:82051152-82051171 Msa0577730:three_prime_UTR 25.0%
! AAATTGTTATGAAGAGATGA+GGG + chr4_1:82051256-82051275 Msa0577730:three_prime_UTR 25.0%
! CGCTTTGTTGAATATTGAAA+TGG - chr4_1:82049434-82049453 None:intergenic 30.0%
AAGAAGAAGAAATTGGATAG+AGG - chr4_1:82049473-82049492 None:intergenic 30.0%
!! CATAATCTATGTTGCTTTCT+TGG + chr4_1:82050000-82050019 Msa0577730:intron 30.0%
TTTCTTGGTTCAAATATTGC+TGG + chr4_1:82050015-82050034 Msa0577730:intron 30.0%
! CAAATATTGCTGGTTAGAAT+TGG + chr4_1:82050025-82050044 Msa0577730:intron 30.0%
ACATTTAAGAGTAGTAGTAG+TGG + chr4_1:82050187-82050206 Msa0577730:intron 30.0%
TGAAGAGACATCAAATACAT+AGG + chr4_1:82050332-82050351 Msa0577730:intron 30.0%
AGACATCAAATACATAGGAT+GGG + chr4_1:82050337-82050356 Msa0577730:intron 30.0%
AAATTATACACTTTCTGTGG+TGG - chr4_1:82050492-82050511 None:intergenic 30.0%
TGCAAATTATACACTTTCTG+TGG - chr4_1:82050495-82050514 None:intergenic 30.0%
GGTCTAGGTACATATTATAT+TGG + chr4_1:82050715-82050734 Msa0577730:intron 30.0%
CATATTATATTGGGAGTAAG+TGG + chr4_1:82050725-82050744 Msa0577730:intron 30.0%
TATTATATTGGGAGTAAGTG+GGG + chr4_1:82050727-82050746 Msa0577730:intron 30.0%
!!! TGCTTTTTTCAGTCAAAGAT+TGG + chr4_1:82050823-82050842 Msa0577730:intron 30.0%
!!! GCTTTTTTCAGTCAAAGATT+GGG + chr4_1:82050824-82050843 Msa0577730:intron 30.0%
! AAAATTGCAACTGTTGTTTG+TGG + chr4_1:82050896-82050915 Msa0577730:three_prime_UTR 30.0%
TGTGGAGTTTGACATTATTA+TGG + chr4_1:82050914-82050933 Msa0577730:three_prime_UTR 30.0%
TTGATTCATAACCCAGATAA+GGG + chr4_1:82051029-82051048 Msa0577730:three_prime_UTR 30.0%
GCATGTCTCTAATAAGATAT+TGG + chr4_1:82051109-82051128 Msa0577730:three_prime_UTR 30.0%
CAAAATAAACAGCAGAAATG+AGG - chr4_1:82051141-82051160 None:intergenic 30.0%
ATAGCATCATTCTGTTATGT+TGG + chr4_1:82051187-82051206 Msa0577730:three_prime_UTR 30.0%
AAAAATCATGTAATGGTGTC+AGG - chr4_1:82051221-82051240 None:intergenic 30.0%
CAACAGCAAAAATCATGTAA+TGG - chr4_1:82051228-82051247 None:intergenic 30.0%
GAAATTGTTATGAAGAGATG+AGG + chr4_1:82051255-82051274 Msa0577730:three_prime_UTR 30.0%
TGCTTCAAATATGAGTTATG+TGG - chr4_1:82051349-82051368 None:intergenic 30.0%
! ACATAACTCATATTTGAAGC+AGG + chr4_1:82051348-82051367 Msa0577730:three_prime_UTR 30.0%
!! TTCTAAAGCACTAAGAATTG+AGG + chr4_1:82051452-82051471 Msa0577730:three_prime_UTR 30.0%
TGTTTGGAAATGAGGAAAAA+AGG - chr4_1:82051573-82051592 None:intergenic 30.0%
ATATGACATGTTTGGAAATG+AGG - chr4_1:82051581-82051600 None:intergenic 30.0%
!! TGTTATAACAGGTATTGATG+TGG + chr4_1:82051735-82051754 Msa0577730:three_prime_UTR 30.0%
TTACGTGAGGAATGGATAAT+TGG - chr4_1:82049296-82049315 None:intergenic 35.0%
!! GAAAAAGTGTTTGACAAGAG+GGG - chr4_1:82049350-82049369 None:intergenic 35.0%
!! GGAAAAAGTGTTTGACAAGA+GGG - chr4_1:82049351-82049370 None:intergenic 35.0%
AGATTGAAAGAGAGGGAAAA+GGG - chr4_1:82049381-82049400 None:intergenic 35.0%
AAGATTGAAAGAGAGGGAAA+AGG - chr4_1:82049382-82049401 None:intergenic 35.0%
AAGACGAAGATTGAAAGAGA+GGG - chr4_1:82049388-82049407 None:intergenic 35.0%
AAAGACGAAGATTGAAAGAG+AGG - chr4_1:82049389-82049408 None:intergenic 35.0%
GATCGATCGGAATAAGATAA+CGG - chr4_1:82049708-82049727 None:intergenic 35.0%
ATAACCAATCCAATAACCCA+CGG - chr4_1:82049882-82049901 None:intergenic 35.0%
! GTTATGGTGTTTTATGTTGC+TGG + chr4_1:82049897-82049916 Msa0577730:CDS 35.0%
CTGCATTAACAATGATCACT+TGG - chr4_1:82050271-82050290 None:intergenic 35.0%
ATGTCTCTTCAACCACATTA+CGG - chr4_1:82050323-82050342 None:intergenic 35.0%
GAGACATCAAATACATAGGA+TGG + chr4_1:82050336-82050355 Msa0577730:intron 35.0%
! TAATTTGCAACACCCATATC+AGG + chr4_1:82050506-82050525 Msa0577730:intron 35.0%
AATTTGCAACACCCATATCA+GGG + chr4_1:82050507-82050526 Msa0577730:intron 35.0%
ATCTAAGCTATCCCTGATAT+GGG - chr4_1:82050521-82050540 None:intergenic 35.0%
TATCTAAGCTATCCCTGATA+TGG - chr4_1:82050522-82050541 None:intergenic 35.0%
AGTAAGTGGGGTTAAGTTTA+TGG + chr4_1:82050739-82050758 Msa0577730:intron 35.0%
GTAAGTGGGGTTAAGTTTAT+GGG + chr4_1:82050740-82050759 Msa0577730:intron 35.0%
TGAATCAAGGACAAACTAAC+TGG - chr4_1:82051017-82051036 None:intergenic 35.0%
CTTATCTGGGTTATGAATCA+AGG - chr4_1:82051030-82051049 None:intergenic 35.0%
CTTGATTCATAACCCAGATA+AGG + chr4_1:82051028-82051047 Msa0577730:three_prime_UTR 35.0%
! TTTTACCTTGACCCTTATCT+GGG - chr4_1:82051043-82051062 None:intergenic 35.0%
ATGAAGAGATGAGGGAAATA+TGG + chr4_1:82051264-82051283 Msa0577730:three_prime_UTR 35.0%
GATAACAAAAGAAAGAAGCC+AGG - chr4_1:82051489-82051508 None:intergenic 35.0%
! ATGATTTGTCTCGAAGAACA+TGG + chr4_1:82051624-82051643 Msa0577730:three_prime_UTR 35.0%
GTACTGGATCCAACAAATTT+CGG + chr4_1:82051649-82051668 Msa0577730:three_prime_UTR 35.0%
!! AACTATTTCTGGGAACAAAG+TGG - chr4_1:82051844-82051863 None:intergenic 35.0%
CATCTCATGTCAATGCTTCA+AGG + chr4_1:82049226-82049245 Msa0577730:five_prime_UTR 40.0%
AAGAAAAACTGGGTTACGTG+AGG - chr4_1:82049309-82049328 None:intergenic 40.0%
!! GGGAAAAAGTGTTTGACAAG+AGG - chr4_1:82049352-82049371 None:intergenic 40.0%
! GAGAGGGAAAAGGGTATAAA+GGG - chr4_1:82049372-82049391 None:intergenic 40.0%
! AGAGAGGGAAAAGGGTATAA+AGG - chr4_1:82049373-82049392 None:intergenic 40.0%
! GATCGGTTACTTTCGTGATT+GGG + chr4_1:82049844-82049863 Msa0577730:CDS 40.0%
!! GTGGGTTATTGGATTGGTTA+TGG + chr4_1:82049881-82049900 Msa0577730:CDS 40.0%
TTGTAGTTAATTGCGGCTGA+TGG + chr4_1:82050295-82050314 Msa0577730:intron 40.0%
TGTAGTTAATTGCGGCTGAT+GGG + chr4_1:82050296-82050315 Msa0577730:intron 40.0%
TTGAGAATTCCATGCTTGAC+AGG + chr4_1:82050694-82050713 Msa0577730:CDS 40.0%
ATGGTGAACTGATTGATGCA+TGG + chr4_1:82050933-82050952 Msa0577730:three_prime_UTR 40.0%
TGGTGAACTGATTGATGCAT+GGG + chr4_1:82050934-82050953 Msa0577730:three_prime_UTR 40.0%
CTAGAATTTGGCTACTCTCT+AGG - chr4_1:82050984-82051003 None:intergenic 40.0%
! CTTTTACCTTGACCCTTATC+TGG - chr4_1:82051044-82051063 None:intergenic 40.0%
AGACATGCTTCAATGAGTTG+AGG - chr4_1:82051097-82051116 None:intergenic 40.0%
!!! TGATTTTTGCTGTTGCAGAG+TGG + chr4_1:82051233-82051252 Msa0577730:three_prime_UTR 40.0%
! CATATTTGAAGCAGGAATCG+TGG + chr4_1:82051356-82051375 Msa0577730:three_prime_UTR 40.0%
! GTTACCATAATTTGTGGGCA+TGG + chr4_1:82051398-82051417 Msa0577730:three_prime_UTR 40.0%
AACAAAAGAAAGAAGCCAGG+CGG - chr4_1:82051486-82051505 None:intergenic 40.0%
CCATCTGATCCGAAATTTGT+TGG - chr4_1:82051661-82051680 None:intergenic 40.0%
CCAACAAATTTCGGATCAGA+TGG + chr4_1:82051658-82051677 Msa0577730:three_prime_UTR 40.0%
! TTTCTGGGAACAAAGTGGTT+TGG - chr4_1:82051839-82051858 None:intergenic 40.0%
AAACTGGGTTACGTGAGGAA+TGG - chr4_1:82049304-82049323 None:intergenic 45.0%
! TTTGACAAGAGGGGTTTGCT+TGG - chr4_1:82049341-82049360 None:intergenic 45.0%
! TCGGAATAAGATAACGGCGT+CGG - chr4_1:82049702-82049721 None:intergenic 45.0%
GGATTTGCGTCGTTTGTTCT+CGG + chr4_1:82049795-82049814 Msa0577730:CDS 45.0%
GATTTGCGTCGTTTGTTCTC+GGG + chr4_1:82049796-82049815 Msa0577730:CDS 45.0%
GTCGTTTGTTCTCGGGATTT+CGG + chr4_1:82049803-82049822 Msa0577730:CDS 45.0%
! GGATCGGTTACTTTCGTGAT+TGG + chr4_1:82049843-82049862 Msa0577730:CDS 45.0%
TTGGGTTTATGTTGATGCCG+TGG + chr4_1:82049862-82049881 Msa0577730:CDS 45.0%
TGGGTTTATGTTGATGCCGT+GGG + chr4_1:82049863-82049882 Msa0577730:CDS 45.0%
! ATGTTGATGCCGTGGGTTAT+TGG + chr4_1:82049870-82049889 Msa0577730:CDS 45.0%
TGGCGAAGCAAAGCAAAGAA+TGG - chr4_1:82049955-82049974 None:intergenic 45.0%
TTACCGGGAGAATCTTTACG+TGG - chr4_1:82049975-82049994 None:intergenic 45.0%
GTAGTTAATTGCGGCTGATG+GGG + chr4_1:82050297-82050316 Msa0577730:intron 45.0%
ATTCCATGCTTGACAGGTCT+AGG + chr4_1:82050700-82050719 Msa0577730:CDS 45.0%
!! AGCCAAATTCTAGCCTTGCT+AGG + chr4_1:82050991-82051010 Msa0577730:three_prime_UTR 45.0%
! ACAAACTAACTGGCCTAGCA+AGG - chr4_1:82051007-82051026 None:intergenic 45.0%
CATAACCCAGATAAGGGTCA+AGG + chr4_1:82051035-82051054 Msa0577730:three_prime_UTR 45.0%
GTTCTATAGAGTGCATGAGC+AGG - chr4_1:82051318-82051337 None:intergenic 45.0%
GAAGCCATGCCCACAAATTA+TGG - chr4_1:82051405-82051424 None:intergenic 45.0%
ACAAAAGAAAGAAGCCAGGC+GGG - chr4_1:82051485-82051504 None:intergenic 45.0%
CTCGAAGAACATGGATGTAC+TGG + chr4_1:82051633-82051652 Msa0577730:three_prime_UTR 45.0%
ACATTGCCACGTCTTTGAGT+GGG + chr4_1:82051801-82051820 Msa0577730:three_prime_UTR 45.0%
! TGGTTGACGACGACGTTTTG+AGG - chr4_1:82049414-82049433 None:intergenic 50.0%
GTTTGTTCTCGGGATTTCGG+TGG + chr4_1:82049806-82049825 Msa0577730:CDS 50.0%
! GGCTTTGATGCTGTGTGGAT+CGG + chr4_1:82049827-82049846 Msa0577730:CDS 50.0%
! GATGCCGTGGGTTATTGGAT+TGG + chr4_1:82049875-82049894 Msa0577730:CDS 50.0%
TCGCCACGTAAAGATTCTCC+CGG + chr4_1:82049969-82049988 Msa0577730:CDS 50.0%
GTACCTAGACCTGTCAAGCA+TGG - chr4_1:82050706-82050725 None:intergenic 50.0%
GGCCTAGCAAGGCTAGAATT+TGG - chr4_1:82050996-82051015 None:intergenic 50.0%
! GGCGTTGAGCAGTACGATAT+TGG + chr4_1:82051285-82051304 Msa0577730:three_prime_UTR 50.0%
CACTCAAAGACGTGGCAATG+TGG - chr4_1:82051802-82051821 None:intergenic 50.0%
CACATTGCCACGTCTTTGAG+TGG + chr4_1:82051800-82051819 Msa0577730:three_prime_UTR 50.0%
! CTCAACACCGACGGATTCAC+CGG - chr4_1:82049741-82049760 None:intergenic 55.0%
GACGAACGTCTCAACACCGA+CGG - chr4_1:82049750-82049769 None:intergenic 55.0%
!! GTCGGTGTTGAGACGTTCGT+CGG + chr4_1:82049749-82049768 Msa0577730:CDS 55.0%
ACAAACGACGCAAATCCCGC+CGG - chr4_1:82049792-82049811 None:intergenic 55.0%
!! TCGGTGGCTTTGATGCTGTG+TGG + chr4_1:82049822-82049841 Msa0577730:CDS 55.0%
TTGCCACGTCTTTGAGTGGG+AGG + chr4_1:82051804-82051823 Msa0577730:three_prime_UTR 55.0%
GTCTTTGAGTGGGAGGAGCT+GGG + chr4_1:82051811-82051830 Msa0577730:three_prime_UTR 55.0%
CGGCACGATCGGAGATCGAT+CGG - chr4_1:82049721-82049740 None:intergenic 60.0%
CGATCGATCTCCGATCGTGC+CGG + chr4_1:82049719-82049738 Msa0577730:CDS 60.0%
! GACGGATTCACCGGCACGAT+CGG - chr4_1:82049732-82049751 None:intergenic 60.0%
! GATCGTGCCGGTGAATCCGT+CGG + chr4_1:82049731-82049750 Msa0577730:CDS 60.0%
! GGTGTTGAGACGTTCGTCGG+CGG + chr4_1:82049752-82049771 Msa0577730:CDS 60.0%
GGCGGCAGGTCAGATAACTC+CGG + chr4_1:82049770-82049789 Msa0577730:CDS 60.0%
GGCAGGTCAGATAACTCCGG+CGG + chr4_1:82049773-82049792 Msa0577730:CDS 60.0%
GCAGGTCAGATAACTCCGGC+GGG + chr4_1:82049774-82049793 Msa0577730:CDS 60.0%
ATTGAGGAGTCGTTCCCGCC+TGG + chr4_1:82051468-82051487 Msa0577730:intron 60.0%
GCTCCTCCCACTCAAAGACG+TGG - chr4_1:82051810-82051829 None:intergenic 60.0%
CGTCTTTGAGTGGGAGGAGC+TGG + chr4_1:82051810-82051829 Msa0577730:three_prime_UTR 60.0%
TTGAGACGTTCGTCGGCGGC+AGG + chr4_1:82049756-82049775 Msa0577730:CDS 65.0%
CGGCTGATGGGGCCGTAATG+TGG + chr4_1:82050308-82050327 Msa0577730:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 82049210 82051912 82049210 ID=Msa0577730;Name=Msa0577730
chr4_1 mRNA 82049210 82051912 82049210 ID=Msa0577730-mRNA-1;Parent=Msa0577730;Name=Msa0577730-mRNA-1;_AED=0.12;_eAED=0.12;_QI=283|0.6|0.83|0.83|0.4|0.16|6|971|157
chr4_1 exon 82049210 82049247 82049210 ID=Msa0577730-mRNA-1:exon:29629;Parent=Msa0577730-mRNA-1
chr4_1 exon 82049271 82049460 82049271 ID=Msa0577730-mRNA-1:exon:29630;Parent=Msa0577730-mRNA-1
chr4_1 exon 82049554 82049990 82049554 ID=Msa0577730-mRNA-1:exon:29631;Parent=Msa0577730-mRNA-1
chr4_1 exon 82050655 82050721 82050655 ID=Msa0577730-mRNA-1:exon:29632;Parent=Msa0577730-mRNA-1
chr4_1 exon 82050835 82051475 82050835 ID=Msa0577730-mRNA-1:exon:29633;Parent=Msa0577730-mRNA-1
chr4_1 exon 82051558 82051912 82051558 ID=Msa0577730-mRNA-1:exon:29634;Parent=Msa0577730-mRNA-1
chr4_1 five_prime_UTR 82049210 82049247 82049210 ID=Msa0577730-mRNA-1:five_prime_utr;Parent=Msa0577730-mRNA-1
chr4_1 five_prime_UTR 82049271 82049460 82049271 ID=Msa0577730-mRNA-1:five_prime_utr;Parent=Msa0577730-mRNA-1
chr4_1 five_prime_UTR 82049554 82049608 82049554 ID=Msa0577730-mRNA-1:five_prime_utr;Parent=Msa0577730-mRNA-1
chr4_1 CDS 82049609 82049990 82049609 ID=Msa0577730-mRNA-1:cds;Parent=Msa0577730-mRNA-1
chr4_1 CDS 82050655 82050721 82050655 ID=Msa0577730-mRNA-1:cds;Parent=Msa0577730-mRNA-1
chr4_1 CDS 82050835 82050859 82050835 ID=Msa0577730-mRNA-1:cds;Parent=Msa0577730-mRNA-1
chr4_1 three_prime_UTR 82050860 82051475 82050860 ID=Msa0577730-mRNA-1:three_prime_utr;Parent=Msa0577730-mRNA-1
chr4_1 three_prime_UTR 82051558 82051912 82051558 ID=Msa0577730-mRNA-1:three_prime_utr;Parent=Msa0577730-mRNA-1
Gene Sequence

>Msa0577730

ATGATGAGAAGAAATCAAGATCAAGAACAACAATCAAAGATGATTTGCGAATTATCATCTCTACTTTTCAATCTTCTTCTACAATCTTCACCGACGCCGTTATCTTATTCCGATCGATCTCCGATCGTGCCGGTGAATCCGTCGGTGTTGAGACGTTCGTCGGCGGCAGGTCAGATAACTCCGGCGGGATTTGCGTCGTTTGTTCTCGGGATTTCGGTGGCTTTGATGCTGTGTGGATCGGTTACTTTCGTGATTGGGTTTATGTTGATGCCGTGGGTTATTGGATTGGTTATGGTGTTTTATGTTGCTGGTGTTGTTTCTTTTCTTTCTGTTTTGGGTCGCTCCATTCTTTGCTTTGCTTCGCCACGTAAAGATTCTCCCGGTTATTGTAGCTTTCATTTTAAAAGGGATATTCATATGGTTGAGAATTCCATGCTTGACAGGTCTAGTCAAAGATTGGGTCTGCAATATTAG
Protein sequence

>Msa0577730

MMRRNQDQEQQSKMICELSSLLFNLLLQSSPTPLSYSDRSPIVPVNPSVLRRSSAAGQITPAGFASFVLGISVALMLCGSVTFVIGFMLMPWVIGLVMVFYVAGVVSFLSVLGRSILCFASPRKDSPGYCSFHFKRDIHMVENSMLDRSSQRLGLQY*