AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0491220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0491220 MtrunA17_Chr3g0082831 90.000 60 5 1 1 59 1 60 7.46e-33 107
Msa0491220 MtrunA17_Chr3g0082821 60.345 58 19 2 1 54 1 58 3.42e-16 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 23 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTCTTATAAATAAATAAA+AGG 0.156298 3_4:+13517838 None:intergenic
GCTTCTTATATCAATTAAAT+TGG 0.203814 3_4:-13518898 Msa0491220:CDS
TAAGAAGCTTATATTGAATA+TGG 0.250103 3_4:-13518934 Msa0491220:CDS
TTTATTTATAAGAATAATAA+TGG 0.286075 3_4:-13517831 Msa0491220:three_prime_UTR
TGGATATATTGACTCAATAT+TGG 0.287423 3_4:-13517865 Msa0491220:three_prime_UTR
CTGCACTGCCTCAATCTATT+AGG 0.314957 3_4:+13517958 None:intergenic
CAACAGCAAGGGTACCCCTT+TGG 0.383801 3_4:-13518875 Msa0491220:CDS
ATTCTTATAAATAAATAAAA+GGG 0.397223 3_4:+13517839 None:intergenic
TCCAGCATATAATTGAGCTT+TGG 0.408952 3_4:+13518012 None:intergenic
TAATTGAGCTTTGGATCCTC+AGG 0.410210 3_4:+13518021 None:intergenic
TTGGATCATCATCACGTAGT+AGG 0.460501 3_4:+13518071 None:intergenic
TGAAAATAGTGGTGGTCTCT+TGG 0.465816 3_4:+13518052 None:intergenic
TCCAAAGCTCAATTATATGC+TGG 0.505060 3_4:-13518013 Msa0491220:three_prime_UTR
AGGGATTATGATGAAAATAG+TGG 0.526545 3_4:+13518041 None:intergenic
AATTAAATTGGCAACAGCAA+GGG 0.558129 3_4:-13518886 Msa0491220:CDS
AAAGAGATCCTAATAGATTG+AGG 0.564683 3_4:-13517966 Msa0491220:three_prime_UTR
TACTTACGTGATTTGAGATG+AGG 0.567266 3_4:+13518843 None:intergenic
ATGAGGCTCAGAAATCCAAA+GGG 0.574064 3_4:+13518860 None:intergenic
AATTGAGCTTTGGATCCTCA+GGG 0.585492 3_4:+13518022 None:intergenic
CAATTAAATTGGCAACAGCA+AGG 0.601125 3_4:-13518887 Msa0491220:CDS
GATGAGGCTCAGAAATCCAA+AGG 0.626210 3_4:+13518859 None:intergenic
GATTATGATGAAAATAGTGG+TGG 0.672789 3_4:+13518044 None:intergenic
TGAGGCTCAGAAATCCAAAG+GGG 0.757467 3_4:+13518861 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAAAAAATAAAA+GGG + chr3_4:13518207-13518226 None:intergenic 0.0%
!! AAAAAAAAAAAAAAATAAAA+GGG + chr3_4:13518207-13518226 None:intergenic 0.0%
!!! TTTTTGATAAAATTAGTTAT+TGG - chr3_4:13518263-13518282 Msa0491220:intron 10.0%
!!! TTACTTGTTTTTATTTTTTA+TGG - chr3_4:13518337-13518356 Msa0491220:intron 10.0%
!!! AACAAATTTTTTTTTTCTTT+GGG + chr3_4:13518627-13518646 None:intergenic 10.0%
!!! AAACAAATTTTTTTTTTCTT+TGG + chr3_4:13518628-13518647 None:intergenic 10.0%
!!! CCAATATTTTATAATTTTTT+TGG + chr3_4:13518904-13518923 None:intergenic 10.0%
!! CCAAAAAAATTATAAAATAT+TGG - chr3_4:13518901-13518920 Msa0491220:CDS 10.0%
!!! AAAAGATATATGTTTTTAAA+CGG + chr3_4:13517817-13517836 None:intergenic 10.0%
!!! TTTTTGATAAAATTAGTTAT+TGG - chr3_4:13518263-13518282 Msa0491220:intron 10.0%
!!! TTACTTGTTTTTATTTTTTA+TGG - chr3_4:13518337-13518356 Msa0491220:intron 10.0%
!!! AACAAATTTTTTTTTTCTTT+GGG + chr3_4:13518627-13518646 None:intergenic 10.0%
!!! AAACAAATTTTTTTTTTCTT+TGG + chr3_4:13518628-13518647 None:intergenic 10.0%
!!! CCAATATTTTATAATTTTTT+TGG + chr3_4:13518904-13518923 None:intergenic 10.0%
!! CCAAAAAAATTATAAAATAT+TGG - chr3_4:13518901-13518920 Msa0491220:CDS 10.0%
!!! TCTATATTTTTTCTTTAAGA+TGG - chr3_4:13517843-13517862 Msa0491220:three_prime_UTR 15.0%
!! TATAAATAGTTCAATCTTAA+AGG + chr3_4:13518033-13518052 None:intergenic 15.0%
!!! GAATAAAATATCTTAGTTTT+TGG - chr3_4:13518229-13518248 Msa0491220:intron 15.0%
!! ATAAGTATATTACAATTTAC+AGG + chr3_4:13518568-13518587 None:intergenic 15.0%
!!! ACAAATTTTTTTTTTCTTTG+GGG + chr3_4:13518626-13518645 None:intergenic 15.0%
!!! AAAAATTTGTTTGCAAATTA+TGG - chr3_4:13518636-13518655 Msa0491220:intron 15.0%
!! TATATCCAGAAAAATTAAAA+AGG + chr3_4:13518930-13518949 None:intergenic 15.0%
!!! TCTATATTTTTTCTTTAAGA+TGG - chr3_4:13517843-13517862 Msa0491220:three_prime_UTR 15.0%
!! TATAAATAGTTCAATCTTAA+AGG + chr3_4:13518033-13518052 None:intergenic 15.0%
!!! GAATAAAATATCTTAGTTTT+TGG - chr3_4:13518229-13518248 Msa0491220:intron 15.0%
!! ATAAGTATATTACAATTTAC+AGG + chr3_4:13518568-13518587 None:intergenic 15.0%
!!! ACAAATTTTTTTTTTCTTTG+GGG + chr3_4:13518626-13518645 None:intergenic 15.0%
!!! AAAAATTTGTTTGCAAATTA+TGG - chr3_4:13518636-13518655 Msa0491220:intron 15.0%
!! TATATCCAGAAAAATTAAAA+AGG + chr3_4:13518930-13518949 None:intergenic 15.0%
!! TAAGAAGCTTATATTGAATA+TGG - chr3_4:13517873-13517892 Msa0491220:three_prime_UTR 20.0%
!! GCTTCTTATATCAATTAAAT+TGG - chr3_4:13517909-13517928 Msa0491220:three_prime_UTR 20.0%
!!! TTTTTACAACCCATATTTTA+GGG + chr3_4:13518072-13518091 None:intergenic 20.0%
!!! ATTTTTACAACCCATATTTT+AGG + chr3_4:13518073-13518092 None:intergenic 20.0%
!!! TGAATGTTTTAGAAATTCAT+TGG - chr3_4:13518135-13518154 Msa0491220:intron 20.0%
!!! GAATGTTTTAGAAATTCATT+GGG - chr3_4:13518136-13518155 Msa0491220:intron 20.0%
!!! AAATTTTTTTTTTCTTTGGG+GGG + chr3_4:13518624-13518643 None:intergenic 20.0%
!!! CAAATTTTTTTTTTCTTTGG+GGG + chr3_4:13518625-13518644 None:intergenic 20.0%
!!! TTGTGTTAATTTTTGCTAAT+TGG - chr3_4:13518664-13518683 Msa0491220:intron 20.0%
!! TAAGAAGCTTATATTGAATA+TGG - chr3_4:13517873-13517892 Msa0491220:three_prime_UTR 20.0%
!! GCTTCTTATATCAATTAAAT+TGG - chr3_4:13517909-13517928 Msa0491220:three_prime_UTR 20.0%
!!! TTTTTACAACCCATATTTTA+GGG + chr3_4:13518072-13518091 None:intergenic 20.0%
!!! ATTTTTACAACCCATATTTT+AGG + chr3_4:13518073-13518092 None:intergenic 20.0%
!!! TGAATGTTTTAGAAATTCAT+TGG - chr3_4:13518135-13518154 Msa0491220:intron 20.0%
!!! GAATGTTTTAGAAATTCATT+GGG - chr3_4:13518136-13518155 Msa0491220:intron 20.0%
!!! AAATTTTTTTTTTCTTTGGG+GGG + chr3_4:13518624-13518643 None:intergenic 20.0%
!!! CAAATTTTTTTTTTCTTTGG+GGG + chr3_4:13518625-13518644 None:intergenic 20.0%
!!! TTGTGTTAATTTTTGCTAAT+TGG - chr3_4:13518664-13518683 Msa0491220:intron 20.0%
!!! GAAAATGTTTTAGAGATACT+AGG + chr3_4:13517991-13518010 None:intergenic 25.0%
!! TTTCATCAAGATTTTGTGAA+GGG - chr3_4:13518098-13518117 Msa0491220:intron 25.0%
!!! TTCTAAAACATTCATTGCAA+AGG + chr3_4:13518129-13518148 None:intergenic 25.0%
! AAAAATAAAAGGGAGTTGAA+TGG + chr3_4:13518197-13518216 None:intergenic 25.0%
!!! AATTTTTTTTTTCTTTGGGG+GGG + chr3_4:13518623-13518642 None:intergenic 25.0%
!!! GGTTTCCTTTTTAATTTTTC+TGG - chr3_4:13518922-13518941 Msa0491220:CDS 25.0%
!!! TGGATATATTGACTCAATAT+TGG - chr3_4:13518942-13518961 Msa0491220:CDS 25.0%
!!! GAAAATGTTTTAGAGATACT+AGG + chr3_4:13517991-13518010 None:intergenic 25.0%
!! TTTCATCAAGATTTTGTGAA+GGG - chr3_4:13518098-13518117 Msa0491220:intron 25.0%
!!! TTCTAAAACATTCATTGCAA+AGG + chr3_4:13518129-13518148 None:intergenic 25.0%
! AAAAATAAAAGGGAGTTGAA+TGG + chr3_4:13518197-13518216 None:intergenic 25.0%
!!! AATTTTTTTTTTCTTTGGGG+GGG + chr3_4:13518623-13518642 None:intergenic 25.0%
!!! GGTTTCCTTTTTAATTTTTC+TGG - chr3_4:13518922-13518941 Msa0491220:CDS 25.0%
!!! TGGATATATTGACTCAATAT+TGG - chr3_4:13518942-13518961 Msa0491220:CDS 25.0%
AATTAAATTGGCAACAGCAA+GGG - chr3_4:13517921-13517940 Msa0491220:three_prime_UTR 30.0%
CTTCACAAAATCTTGATGAA+AGG + chr3_4:13518099-13518118 None:intergenic 30.0%
! CTTTCATCAAGATTTTGTGA+AGG - chr3_4:13518097-13518116 Msa0491220:intron 30.0%
TTCATTTGATCCACACATAT+TGG + chr3_4:13518483-13518502 None:intergenic 30.0%
!! TGCTATATTATTGACTCACT+AGG - chr3_4:13518511-13518530 Msa0491220:intron 30.0%
!!! ATTTTTTTTTTCTTTGGGGG+GGG + chr3_4:13518622-13518641 None:intergenic 30.0%
!! TATTTTTGTGACCTGTAAGA+TGG - chr3_4:13518710-13518729 Msa0491220:intron 30.0%
GATTATGATGAAAATAGTGG+TGG + chr3_4:13518766-13518785 None:intergenic 30.0%
AGGGATTATGATGAAAATAG+TGG + chr3_4:13518769-13518788 None:intergenic 30.0%
! TATTTTCATCATAATCCCTG+AGG - chr3_4:13518770-13518789 Msa0491220:intron 30.0%
AAAGAGATCCTAATAGATTG+AGG - chr3_4:13518841-13518860 Msa0491220:intron 30.0%
AATTAAATTGGCAACAGCAA+GGG - chr3_4:13517921-13517940 Msa0491220:three_prime_UTR 30.0%
CTTCACAAAATCTTGATGAA+AGG + chr3_4:13518099-13518118 None:intergenic 30.0%
! CTTTCATCAAGATTTTGTGA+AGG - chr3_4:13518097-13518116 Msa0491220:intron 30.0%
TTCATTTGATCCACACATAT+TGG + chr3_4:13518483-13518502 None:intergenic 30.0%
!! TGCTATATTATTGACTCACT+AGG - chr3_4:13518511-13518530 Msa0491220:intron 30.0%
!!! ATTTTTTTTTTCTTTGGGGG+GGG + chr3_4:13518622-13518641 None:intergenic 30.0%
!! TATTTTTGTGACCTGTAAGA+TGG - chr3_4:13518710-13518729 Msa0491220:intron 30.0%
GATTATGATGAAAATAGTGG+TGG + chr3_4:13518766-13518785 None:intergenic 30.0%
AGGGATTATGATGAAAATAG+TGG + chr3_4:13518769-13518788 None:intergenic 30.0%
! TATTTTCATCATAATCCCTG+AGG - chr3_4:13518770-13518789 Msa0491220:intron 30.0%
AAAGAGATCCTAATAGATTG+AGG - chr3_4:13518841-13518860 Msa0491220:intron 30.0%
CAATTAAATTGGCAACAGCA+AGG - chr3_4:13517920-13517939 Msa0491220:three_prime_UTR 35.0%
! TACTTACGTGATTTGAGATG+AGG + chr3_4:13517967-13517986 None:intergenic 35.0%
TTCCAGAAAGCCCTAAAATA+TGG - chr3_4:13518059-13518078 Msa0491220:CDS 35.0%
TCCAGAAAGCCCTAAAATAT+GGG - chr3_4:13518060-13518079 Msa0491220:CDS 35.0%
AGATTATCTTGGGTCAAGTT+GGG - chr3_4:13518166-13518185 Msa0491220:intron 35.0%
ATTTGATCCACACATATTGG+CGG + chr3_4:13518480-13518499 None:intergenic 35.0%
TCAATCTTGTGAACTACACA+TGG - chr3_4:13518540-13518559 Msa0491220:intron 35.0%
CAATCTTGTGAACTACACAT+GGG - chr3_4:13518541-13518560 Msa0491220:intron 35.0%
!!! TTTTTTTTTTCTTTGGGGGG+GGG + chr3_4:13518621-13518640 None:intergenic 35.0%
TCCAAAGCTCAATTATATGC+TGG - chr3_4:13518794-13518813 Msa0491220:intron 35.0%
TCCAGCATATAATTGAGCTT+TGG + chr3_4:13518798-13518817 None:intergenic 35.0%
CAATTAAATTGGCAACAGCA+AGG - chr3_4:13517920-13517939 Msa0491220:three_prime_UTR 35.0%
! TACTTACGTGATTTGAGATG+AGG + chr3_4:13517967-13517986 None:intergenic 35.0%
TTCCAGAAAGCCCTAAAATA+TGG - chr3_4:13518059-13518078 Msa0491220:CDS 35.0%
TCCAGAAAGCCCTAAAATAT+GGG - chr3_4:13518060-13518079 Msa0491220:CDS 35.0%
AGATTATCTTGGGTCAAGTT+GGG - chr3_4:13518166-13518185 Msa0491220:intron 35.0%
ATTTGATCCACACATATTGG+CGG + chr3_4:13518480-13518499 None:intergenic 35.0%
TCAATCTTGTGAACTACACA+TGG - chr3_4:13518540-13518559 Msa0491220:intron 35.0%
CAATCTTGTGAACTACACAT+GGG - chr3_4:13518541-13518560 Msa0491220:intron 35.0%
!!! TTTTTTTTTTCTTTGGGGGG+GGG + chr3_4:13518621-13518640 None:intergenic 35.0%
TCCAAAGCTCAATTATATGC+TGG - chr3_4:13518794-13518813 Msa0491220:intron 35.0%
TCCAGCATATAATTGAGCTT+TGG + chr3_4:13518798-13518817 None:intergenic 35.0%
ATGAGGCTCAGAAATCCAAA+GGG + chr3_4:13517950-13517969 None:intergenic 40.0%
!!! ACCCATATTTTAGGGCTTTC+TGG + chr3_4:13518064-13518083 None:intergenic 40.0%
TGACCCAAGATAATCTGTCT+CGG + chr3_4:13518162-13518181 None:intergenic 40.0%
CAGATTATCTTGGGTCAAGT+TGG - chr3_4:13518165-13518184 Msa0491220:intron 40.0%
TTTGATCCACACATATTGGC+GGG + chr3_4:13518479-13518498 None:intergenic 40.0%
!!! TTTTTTTTTCTTTGGGGGGG+GGG + chr3_4:13518620-13518639 None:intergenic 40.0%
TTGGATCATCATCACGTAGT+AGG + chr3_4:13518739-13518758 None:intergenic 40.0%
TGAAAATAGTGGTGGTCTCT+TGG + chr3_4:13518758-13518777 None:intergenic 40.0%
!! AATTGAGCTTTGGATCCTCA+GGG + chr3_4:13518788-13518807 None:intergenic 40.0%
!! TAATTGAGCTTTGGATCCTC+AGG + chr3_4:13518789-13518808 None:intergenic 40.0%
ATGAGGCTCAGAAATCCAAA+GGG + chr3_4:13517950-13517969 None:intergenic 40.0%
!!! ACCCATATTTTAGGGCTTTC+TGG + chr3_4:13518064-13518083 None:intergenic 40.0%
TGACCCAAGATAATCTGTCT+CGG + chr3_4:13518162-13518181 None:intergenic 40.0%
CAGATTATCTTGGGTCAAGT+TGG - chr3_4:13518165-13518184 Msa0491220:intron 40.0%
TTTGATCCACACATATTGGC+GGG + chr3_4:13518479-13518498 None:intergenic 40.0%
!!! TTTTTTTTTCTTTGGGGGGG+GGG + chr3_4:13518620-13518639 None:intergenic 40.0%
TTGGATCATCATCACGTAGT+AGG + chr3_4:13518739-13518758 None:intergenic 40.0%
TGAAAATAGTGGTGGTCTCT+TGG + chr3_4:13518758-13518777 None:intergenic 40.0%
!! AATTGAGCTTTGGATCCTCA+GGG + chr3_4:13518788-13518807 None:intergenic 40.0%
!! TAATTGAGCTTTGGATCCTC+AGG + chr3_4:13518789-13518808 None:intergenic 40.0%
TGAGGCTCAGAAATCCAAAG+GGG + chr3_4:13517949-13517968 None:intergenic 45.0%
GATGAGGCTCAGAAATCCAA+AGG + chr3_4:13517951-13517970 None:intergenic 45.0%
ATCAAACCCGCCAATATGTG+TGG - chr3_4:13518470-13518489 Msa0491220:intron 45.0%
!!! TTTTTTTTCTTTGGGGGGGG+GGG + chr3_4:13518619-13518638 None:intergenic 45.0%
CTGCACTGCCTCAATCTATT+AGG + chr3_4:13518852-13518871 None:intergenic 45.0%
TGAGGCTCAGAAATCCAAAG+GGG + chr3_4:13517949-13517968 None:intergenic 45.0%
GATGAGGCTCAGAAATCCAA+AGG + chr3_4:13517951-13517970 None:intergenic 45.0%
ATCAAACCCGCCAATATGTG+TGG - chr3_4:13518470-13518489 Msa0491220:intron 45.0%
!!! TTTTTTTTCTTTGGGGGGGG+GGG + chr3_4:13518619-13518638 None:intergenic 45.0%
CTGCACTGCCTCAATCTATT+AGG + chr3_4:13518852-13518871 None:intergenic 45.0%
!! CAAAAAAAAAAAAAAATAAA+AGG + chr3_4:13518208-13518227 None:intergenic 5.0%
!!! TAATTTTTTATTTTTCTTTT+TGG - chr3_4:13518433-13518452 Msa0491220:intron 5.0%
!! ATTCTTATAAATAAATAAAA+GGG + chr3_4:13518971-13518990 None:intergenic 5.0%
!! TATTCTTATAAATAAATAAA+AGG + chr3_4:13518972-13518991 None:intergenic 5.0%
!! TTTATTTATAAGAATAATAA+TGG - chr3_4:13518976-13518995 Msa0491220:five_prime_UTR 5.0%
!! CAAAAAAAAAAAAAAATAAA+AGG + chr3_4:13518208-13518227 None:intergenic 5.0%
!!! TAATTTTTTATTTTTCTTTT+TGG - chr3_4:13518433-13518452 Msa0491220:intron 5.0%
!! ATTCTTATAAATAAATAAAA+GGG + chr3_4:13518971-13518990 None:intergenic 5.0%
!! TATTCTTATAAATAAATAAA+AGG + chr3_4:13518972-13518991 None:intergenic 5.0%
!! TTTATTTATAAGAATAATAA+TGG - chr3_4:13518976-13518995 Msa0491220:five_prime_UTR 5.0%
TGGGCCGAGACAGATTATCT+TGG - chr3_4:13518155-13518174 Msa0491220:intron 50.0%
GGGCCGAGACAGATTATCTT+GGG - chr3_4:13518156-13518175 Msa0491220:intron 50.0%
!!! TTTTTTTCTTTGGGGGGGGG+GGG + chr3_4:13518618-13518637 None:intergenic 50.0%
GTAGTAGGCGTCCATCTTAC+AGG + chr3_4:13518724-13518743 None:intergenic 50.0%
TGGGCCGAGACAGATTATCT+TGG - chr3_4:13518155-13518174 Msa0491220:intron 50.0%
GGGCCGAGACAGATTATCTT+GGG - chr3_4:13518156-13518175 Msa0491220:intron 50.0%
!!! TTTTTTTCTTTGGGGGGGGG+GGG + chr3_4:13518618-13518637 None:intergenic 50.0%
GTAGTAGGCGTCCATCTTAC+AGG + chr3_4:13518724-13518743 None:intergenic 50.0%
!! CAACAGCAAGGGTACCCCTT+TGG - chr3_4:13517932-13517951 Msa0491220:three_prime_UTR 55.0%
!!! TTTTTTCTTTGGGGGGGGGG+GGG + chr3_4:13518617-13518636 None:intergenic 55.0%
!! CAACAGCAAGGGTACCCCTT+TGG - chr3_4:13517932-13517951 Msa0491220:three_prime_UTR 55.0%
!!! TTTTTTCTTTGGGGGGGGGG+GGG + chr3_4:13518617-13518636 None:intergenic 55.0%
!! TTTTTCTTTGGGGGGGGGGG+GGG + chr3_4:13518616-13518635 None:intergenic 60.0%
!! TTTTTCTTTGGGGGGGGGGG+GGG + chr3_4:13518616-13518635 None:intergenic 60.0%
!! TTTTCTTTGGGGGGGGGGGG+GGG + chr3_4:13518615-13518634 None:intergenic 65.0%
!! TTTTCTTTGGGGGGGGGGGG+GGG + chr3_4:13518615-13518634 None:intergenic 65.0%
! TTTCTTTGGGGGGGGGGGGG+GGG + chr3_4:13518614-13518633 None:intergenic 70.0%
! TTTCTTTGGGGGGGGGGGGG+GGG + chr3_4:13518614-13518633 None:intergenic 70.0%
! TTCTTTGGGGGGGGGGGGGG+GGG + chr3_4:13518613-13518632 None:intergenic 75.0%
! TTCTTTGGGGGGGGGGGGGG+GGG + chr3_4:13518613-13518632 None:intergenic 75.0%
!! TCTTTGGGGGGGGGGGGGGG+GGG + chr3_4:13518612-13518631 None:intergenic 80.0%
!! TCTTTGGGGGGGGGGGGGGG+GGG + chr3_4:13518612-13518631 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 13517816 13519013 13517816 ID=Msa0491220;Name=Msa0491220
chr3_4 mRNA 13517816 13519013 13517816 ID=Msa0491220-mRNA-1;Parent=Msa0491220;Name=Msa0491220-mRNA-1;_AED=0.00;_eAED=0.00;_QI=46|1|1|1|0|0.5|2|223|59
chr3_4 exon 13517816 13518100 13517816 ID=Msa0491220-mRNA-1:exon:4096;Parent=Msa0491220-mRNA-1
chr3_4 exon 13518850 13519013 13518850 ID=Msa0491220-mRNA-1:exon:4095;Parent=Msa0491220-mRNA-1
chr3_4 five_prime_UTR 13518968 13519013 13518968 ID=Msa0491220-mRNA-1:five_prime_utr;Parent=Msa0491220-mRNA-1
chr3_4 CDS 13518850 13518967 13518850 ID=Msa0491220-mRNA-1:cds;Parent=Msa0491220-mRNA-1
chr3_4 CDS 13518039 13518100 13518039 ID=Msa0491220-mRNA-1:cds;Parent=Msa0491220-mRNA-1
chr3_4 three_prime_UTR 13517816 13518038 13517816 ID=Msa0491220-mRNA-1:three_prime_utr;Parent=Msa0491220-mRNA-1
Gene Sequence

>Msa0491220

ATGGCTTACTTTAAGAAGCTTATATTGAATATGGCTTTACTGTTCGTGCTTCTTATATCAATTAAATTGGCAACAGCAAGGGTACCCCTTTGGATTTCTGAGCCTCATCTCAAATCACATGGACGCCTACTACGTGATGATGATCCAAGAGACCACCACTATTTTCATCATAATCCCTGA
Protein sequence

>Msa0491220

MAYFKKLILNMALLFVLLISIKLATARVPLWISEPHLKSHGRLLRDDDPRDHHYFHHNP*