AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0648860


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0648860 MtrunA17_Chr4g0040491 96.203 79 3 0 1 79 1 79 7.28e-49 149
Msa0648860 MtrunA17_Chr2g0324911 72.727 77 21 0 3 79 2 78 3.29e-29 99.4
Msa0648860 MtrunA17_Chr2g0283451 55.405 74 33 0 6 79 7 80 6.86e-26 90.9
Msa0648860 MtrunA17_Chr4g0040991 54.930 71 32 0 9 79 7 77 3.38e-24 86.7
Msa0648860 MtrunA17_Chr2g0325441 54.930 71 32 0 9 79 7 77 6.17e-24 85.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0648860 AT2G20562.1 61.972 71 27 0 9 79 3 73 2.75e-21 79.3
Msa0648860 AT2G31090.1 60.563 71 28 0 9 79 5 75 3.41e-20 76.6

Find 21 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTTATAGGTGCCATTATT+TGG 0.130092 4_3:+55396341 Msa0648860:CDS
CCACAAAACTAATTCCAAAT+TGG 0.179203 4_3:-55396684 None:intergenic
ATATAAAGAGCTTCTAATTA+AGG 0.252543 4_3:+55396763 Msa0648860:three_prime_UTR
TTAACAAAATTCCTTGTTAG+AGG 0.284855 4_3:+55396611 Msa0648860:CDS
CCATTGATATTATGTCAAAT+TGG 0.290690 4_3:-55396237 None:intergenic
AGACCTTTGGGTTTCTTCAT+TGG 0.312975 4_3:+55396286 Msa0648860:CDS
CACAAAACTAATTCCAAATT+GGG 0.364960 4_3:-55396683 None:intergenic
GCTTGATGTTGCTGTATGTT+TGG 0.367654 4_3:+55396555 Msa0648860:CDS
TTCCAGTTAGAATTCTTAGA+TGG 0.387506 4_3:+55396584 Msa0648860:CDS
AACCATCTAAGAATTCTAAC+TGG 0.391220 4_3:-55396586 None:intergenic
GGTGCCATTATTTGGATTCT+CGG 0.429725 4_3:+55396349 Msa0648860:CDS
CCAATTTGACATAATATCAA+TGG 0.432166 4_3:+55396237 Msa0648860:exon
ATTAATGATAATAGAGCAAA+GGG 0.435160 4_3:-55396313 None:intergenic
GTGCCATTATTTGGATTCTC+GGG 0.441384 4_3:+55396350 Msa0648860:CDS
ATACCCGAGAATCCAAATAA+TGG 0.476544 4_3:-55396353 None:intergenic
CCTTGTTGCATATGCTGCAC+AGG 0.484811 4_3:+55396529 Msa0648860:CDS
GTCTACATTCACAAAAGTCT+TGG 0.513894 4_3:-55396267 None:intergenic
AATTAATGATAATAGAGCAA+AGG 0.578873 4_3:-55396314 None:intergenic
CCTGTGCAGCATATGCAACA+AGG 0.629974 4_3:-55396529 None:intergenic
ATAGTACTTATCCTCTAACA+AGG 0.686710 4_3:-55396622 None:intergenic
AATCCAATGAAGAAACCCAA+AGG 0.693801 4_3:-55396289 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCATTAATTTTATCTCTTAT+AGG + chr4_3:55396328-55396347 Msa0648860:CDS 15.0%
!! ATTAATGATAATAGAGCAAA+GGG - chr4_3:55396316-55396335 None:intergenic 20.0%
!! AATTAATGATAATAGAGCAA+AGG - chr4_3:55396317-55396336 None:intergenic 20.0%
!! ACTACAAAAACATAGAAAAT+GGG - chr4_3:55396404-55396423 None:intergenic 20.0%
!! TACTACAAAAACATAGAAAA+TGG - chr4_3:55396405-55396424 None:intergenic 20.0%
!!! TGTTCATCTTCTTTTATTAT+AGG + chr4_3:55396485-55396504 Msa0648860:intron 20.0%
!!! TATATTTTTGTATCCCAATT+TGG + chr4_3:55396670-55396689 Msa0648860:three_prime_UTR 20.0%
!! ATATAAAGAGCTTCTAATTA+AGG + chr4_3:55396763-55396782 Msa0648860:three_prime_UTR 20.0%
! CCATTGATATTATGTCAAAT+TGG - chr4_3:55396240-55396259 None:intergenic 25.0%
! CCAATTTGACATAATATCAA+TGG + chr4_3:55396237-55396256 Msa0648860:exon 25.0%
! TTAACAAAATTCCTTGTTAG+AGG + chr4_3:55396611-55396630 Msa0648860:CDS 25.0%
! GGGATACAAAAATATAAAGA+AGG - chr4_3:55396666-55396685 None:intergenic 25.0%
! CACAAAACTAATTCCAAATT+GGG - chr4_3:55396686-55396705 None:intergenic 25.0%
!!! CAATTTGGAATTAGTTTTGT+GGG + chr4_3:55396685-55396704 Msa0648860:three_prime_UTR 25.0%
! TTTTGTGAATGTAGACCTTT+GGG + chr4_3:55396274-55396293 Msa0648860:CDS 30.0%
TTCCAGTTAGAATTCTTAGA+TGG + chr4_3:55396584-55396603 Msa0648860:CDS 30.0%
AACCATCTAAGAATTCTAAC+TGG - chr4_3:55396589-55396608 None:intergenic 30.0%
ATAGTACTTATCCTCTAACA+AGG - chr4_3:55396625-55396644 None:intergenic 30.0%
CCACAAAACTAATTCCAAAT+TGG - chr4_3:55396687-55396706 None:intergenic 30.0%
!!! CCAATTTGGAATTAGTTTTG+TGG + chr4_3:55396684-55396703 Msa0648860:three_prime_UTR 30.0%
GTCTACATTCACAAAAGTCT+TGG - chr4_3:55396270-55396289 None:intergenic 35.0%
! CTTTTGTGAATGTAGACCTT+TGG + chr4_3:55396273-55396292 Msa0648860:CDS 35.0%
AATCCAATGAAGAAACCCAA+AGG - chr4_3:55396292-55396311 None:intergenic 35.0%
!! CTCTTATAGGTGCCATTATT+TGG + chr4_3:55396341-55396360 Msa0648860:CDS 35.0%
ATACCCGAGAATCCAAATAA+TGG - chr4_3:55396356-55396375 None:intergenic 35.0%
! AGACCTTTGGGTTTCTTCAT+TGG + chr4_3:55396286-55396305 Msa0648860:CDS 40.0%
!! GGTGCCATTATTTGGATTCT+CGG + chr4_3:55396349-55396368 Msa0648860:CDS 40.0%
! GTGCCATTATTTGGATTCTC+GGG + chr4_3:55396350-55396369 Msa0648860:CDS 40.0%
GCTTGATGTTGCTGTATGTT+TGG + chr4_3:55396555-55396574 Msa0648860:CDS 40.0%
CCTGTGCAGCATATGCAACA+AGG - chr4_3:55396532-55396551 None:intergenic 50.0%
CCTTGTTGCATATGCTGCAC+AGG + chr4_3:55396529-55396548 Msa0648860:CDS 50.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 55396235 55396890 55396235 ID=Msa0648860;Name=Msa0648860
chr4_3 mRNA 55396235 55396890 55396235 ID=Msa0648860-mRNA-1;Parent=Msa0648860;Name=Msa0648860-mRNA-1;_AED=0.10;_eAED=0.10;_QI=21|1|1|1|1|1|2|260|79
chr4_3 exon 55396235 55396371 55396235 ID=Msa0648860-mRNA-1:exon:14180;Parent=Msa0648860-mRNA-1
chr4_3 exon 55396507 55396890 55396507 ID=Msa0648860-mRNA-1:exon:14181;Parent=Msa0648860-mRNA-1
chr4_3 five_prime_UTR 55396235 55396255 55396235 ID=Msa0648860-mRNA-1:five_prime_utr;Parent=Msa0648860-mRNA-1
chr4_3 CDS 55396256 55396371 55396256 ID=Msa0648860-mRNA-1:cds;Parent=Msa0648860-mRNA-1
chr4_3 CDS 55396507 55396630 55396507 ID=Msa0648860-mRNA-1:cds;Parent=Msa0648860-mRNA-1
chr4_3 three_prime_UTR 55396631 55396890 55396631 ID=Msa0648860-mRNA-1:three_prime_utr;Parent=Msa0648860-mRNA-1
Gene Sequence

>Msa0648860

ATGGCAAGTGACCAAGACTTTTGTGAATGTAGACCTTTGGGTTTCTTCATTGGATTACCCTTTGCTCTATTATCATTAATTTTATCTCTTATAGGTGCCATTATTTGGATTCTCGGGTCAATATTGAGTTGCTGCTGTCCTTGTTGCATATGCTGCACAGGATTGCTTGATGTTGCTGTATGTTTGGTAAAGCTTCCAGTTAGAATTCTTAGATGGTTTGTTAACAAAATTCCTTGTTAG

Protein sequence

>Msa0648860

MASDQDFCECRPLGFFIGLPFALLSLILSLIGAIIWILGSILSCCCPCCICCTGLLDVAVCLVKLPVRILRWFVNKIPC*