AlfalfaGEDB | Msa0842650

M. sativa cultivar zhongmu-4 / Msa0842650

KEGG: -
GO: GO:0000302 GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009506 GO:0009987 GO:0010035 GO:0010193 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0030054 GO:0036211 GO:0042221 GO:0042493 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0046677 GO:0046777 GO:0050896 GO:0055044 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:1901700
PFAM: PF01657.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0842650	XP_024639191.1	94.599	648	32	1	1	645	1	648	0.0	1236

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0842650	sp\|Q9CAL3\|CRK2_ARATH	64.427	655	216	7	2	647	3	649	0.0	825

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0842650	G7K057	92.747	648	31	2	1	645	1	635	0.0	1201

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0002230	Msa0842650	0.832701	8.134158e-56	-8.615850e-47
Msa0002240	Msa0842650	0.836216	1.065435e-56	-8.615850e-47
Msa0003890	Msa0842650	0.814294	1.657836e-51	-8.615850e-47
Msa0006070	Msa0842650	0.817329	3.489979e-52	-8.615850e-47
Msa0006110	Msa0842650	0.830689	2.549754e-55	-8.615850e-47
Msa0006390	Msa0842650	0.806571	7.712078e-50	-8.615850e-47
Msa0007080	Msa0842650	0.822634	2.132545e-53	-8.615850e-47
Msa0007280	Msa0842650	0.828495	8.708897e-55	-8.615850e-47
Msa0010890	Msa0842650	0.807319	5.356980e-50	-8.615850e-47
Msa0011170	Msa0842650	0.821804	3.322279e-53	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0842650	MtrunA17_Chr5g0413171	94.660	412	19	1	237	645	1	412	0.0	768
Msa0842650	MtrunA17_Chr4g0058891	38.322	608	311	13	21	613	14	572	1.38e-131	400
Msa0842650	MtrunA17_Chr3g0109431	39.353	587	322	15	47	613	45	617	1.55e-125	385
Msa0842650	MtrunA17_Chr7g0226891	52.722	349	149	5	274	618	4	340	1.22e-124	374
Msa0842650	MtrunA17_Chr3g0109411	38.870	602	345	14	29	615	23	616	1.41e-124	383
Msa0842650	MtrunA17_Chr3g0109461	40.072	554	296	15	84	613	89	630	2.84e-122	377
Msa0842650	MtrunA17_Chr1g0206361	36.516	597	340	13	47	612	43	631	7.08e-119	369
Msa0842650	MtrunA17_Chr5g0413161	94.048	168	10	0	1	168	1	168	3.85e-113	336
Msa0842650	MtrunA17_Chr1g0205771	35.943	562	325	12	71	609	79	628	1.30e-109	345
Msa0842650	MtrunA17_Chr8g0354271	36.760	574	325	14	63	611	63	623	1.81e-107	338

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0842650	AT1G70520.1	64.427	655	216	7	2	647	3	649	0.0	825
Msa0842650	AT1G70530.1	41.639	610	319	11	28	617	19	611	5.81e-161	477
Msa0842650	AT5G40380.1	43.328	577	297	11	51	617	53	609	9.71e-161	476
Msa0842650	AT4G28670.1	41.872	609	317	9	32	615	28	624	7.02e-155	461
Msa0842650	AT1G70530.2	44.272	515	258	8	28	522	19	524	3.18e-147	438
Msa0842650	AT5G40380.2	46.680	482	229	10	51	522	53	516	1.09e-145	434
Msa0842650	AT5G40380.3	42.949	468	238	11	30	486	31	480	7.79e-125	378
Msa0842650	AT1G70530.3	42.171	479	248	8	28	486	19	488	1.98e-124	377
Msa0842650	AT4G23230.1	37.457	582	314	15	68	615	62	627	7.03e-108	339
Msa0842650	AT4G05200.1	33.588	655	377	18	9	621	5	643	6.04e-104	330

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Find 121 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGATATGAGATTATTAT+TGG	0.199830	5_4:+19800062	Msa0842650:CDS
AAAAGCCAGCAACATCCTAT+TGG	0.407864	5_4:+19800148	Msa0842650:CDS
AAACTAGGGCATGGAGGATT+CGG	0.417682	5_4:+19799613	Msa0842650:CDS
AAAGAAGATAGAGCTGTGTG+TGG	0.636944	5_4:+19798737	Msa0842650:CDS
AAAGATATGAGATTATTATT+GGG	0.406221	5_4:+19800063	Msa0842650:CDS
AAATGAATCTCTTTATGTTC+TGG	0.449285	5_4:+19798883	Msa0842650:CDS
AAATGCGACCGGAGTTATAA+GGG	0.481982	5_4:-19798655	None:intergenic
AACAAGATGCTCTTCATTCT+TGG	0.256604	5_4:-19801168	Msa0842660:three_prime_UTR
AACACAACTAAGAAGAGCTT+GGG	0.475200	5_4:+19798761	Msa0842650:CDS
AACATAAAGAGATTCATTTG+CGG	0.497153	5_4:-19798879	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGTTTATCAAGTCAAGTCA+AGG	+	chr5_4:19798177-19798196	Msa0842650:five_prime_UTR	30.0%
AAGTCAAGTCAAGGATATAT+AGG	+	chr5_4:19798186-19798205	Msa0842650:five_prime_UTR	30.0%
CTCATTTCATCATAGTTTGA+AGG	-	chr5_4:19798238-19798257	None:intergenic	30.0%
ACTTGATTAGAAGTAGTTTG+AGG	-	chr5_4:19798355-19798374	None:intergenic	30.0%
TCCATAGTTGCAACAAAATT+CGG	-	chr5_4:19798476-19798495	None:intergenic	30.0%
TTAACAAGGAAGTGGAAAAT+GGG	+	chr5_4:19799050-19799069	Msa0842650:CDS	30.0%
CATAAATGGTGTGATATTCT+AGG	-	chr5_4:19799354-19799373	None:intergenic	30.0%
CTTATCTTGCAAACAAATTG+TGG	+	chr5_4:19799416-19799435	Msa0842650:intron	30.0%
TGCTTGATATGTAAAACTTG+TGG	-	chr5_4:19799683-19799702	None:intergenic	30.0%
TATCAAGCAGATTAACAGTT+TGG	+	chr5_4:19799694-19799713	Msa0842650:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_4	gene	19798143	19801335	19798143	ID=Msa0842650;Name=Msa0842650
chr5_4	mRNA	19798143	19801335	19798143	ID=Msa0842650-mRNA-1;Parent=Msa0842650;Name=Msa0842650-mRNA-1;_AED=0.03;_eAED=0.03;_QI=174\|0.66\|0.57\|1\|1\|1\|7\|13\|647
chr5_4	exon	19798143	19799082	19798143	ID=Msa0842650-mRNA-1:exon:8234;Parent=Msa0842650-mRNA-1
chr5_4	exon	19799184	19799297	19799184	ID=Msa0842650-mRNA-1:exon:8235;Parent=Msa0842650-mRNA-1
chr5_4	exon	19799509	19799639	19799509	ID=Msa0842650-mRNA-1:exon:8236;Parent=Msa0842650-mRNA-1
chr5_4	exon	19799726	19799936	19799726	ID=Msa0842650-mRNA-1:exon:8237;Parent=Msa0842650-mRNA-1
chr5_4	exon	19800030	19800264	19800030	ID=Msa0842650-mRNA-1:exon:8238;Parent=Msa0842650-mRNA-1
chr5_4	exon	19800739	19800889	19800739	ID=Msa0842650-mRNA-1:exon:8239;Parent=Msa0842650-mRNA-1
chr5_4	exon	19800987	19801335	19800987	ID=Msa0842650-mRNA-1:exon:8240;Parent=Msa0842650-mRNA-1
chr5_4	five_prime_UTR	19798143	19798316	19798143	ID=Msa0842650-mRNA-1:five_prime_utr;Parent=Msa0842650-mRNA-1
chr5_4	CDS	19798317	19799082	19798317	ID=Msa0842650-mRNA-1:cds;Parent=Msa0842650-mRNA-1
chr5_4	CDS	19799184	19799297	19799184	ID=Msa0842650-mRNA-1:cds;Parent=Msa0842650-mRNA-1
chr5_4	CDS	19799509	19799639	19799509	ID=Msa0842650-mRNA-1:cds;Parent=Msa0842650-mRNA-1
chr5_4	CDS	19799726	19799936	19799726	ID=Msa0842650-mRNA-1:cds;Parent=Msa0842650-mRNA-1
chr5_4	CDS	19800030	19800264	19800030	ID=Msa0842650-mRNA-1:cds;Parent=Msa0842650-mRNA-1
chr5_4	CDS	19800739	19800889	19800739	ID=Msa0842650-mRNA-1:cds;Parent=Msa0842650-mRNA-1
chr5_4	CDS	19800987	19801322	19800987	ID=Msa0842650-mRNA-1:cds;Parent=Msa0842650-mRNA-1
chr5_4	three_prime_UTR	19801323	19801335	19801323	ID=Msa0842650-mRNA-1:three_prime_utr;Parent=Msa0842650-mRNA-1

Gene Sequence

>Msa0842650

ATGAAGAAACCAGTCCCTCTCTTTTTTTCTCAATGCCTCAAACTACTTCTAATCAAGTTTGCACTACTATCAGAAACTATAATCGCAGAACCAAGAGCAAAGACAGTTATGATAATATGTGGCCATCAAGTAGAGCATAACACAAGCATCTTTGTTCCGAATTTTGTTGCAACTATGGAGAAGATTAGCGAGCAGATGCGCAGTACCGGCTTTGGCACAGCAGTTACAGGGACAGGACCTGATGATAACTATGGCCTAGCACAATGCTATGGAGATCTTTCATTACTTGACTGTGTGTTGTGTTATGCTGAGGCGCGCACGGTTCTTCCTCAGTGCTTCCCTTATAACTCCGGTCGCATTTACCTTGATGGGTGCTTCATGAGGTCTGCGAATTATAGTTTTTTTAATGAGTATACAGGAAAAGAAGATAGAGCTGTGTGTGGGAACACAACTAAGAAGAGCTTGGGTTTTCAAGTAGCAGCGAAGGAGGCAGTGTTGAAAGCGGTTCGAGATGCACCGAAAAATAAAGGCTATGCCAGAGAGAAAGTAGTTGTATCAGGAACCGCAAATGAATCTCTTTATGTTCTGGCTGATTGTTGGAGAAGTTTGGATTCTAAGTCTTGCAAAGCCTGTCTCGAGCATGCGTCGTCTTCTATTTTGAGATGTCTTCCCTCATCAGAAGGACGGGCGCTCAACACCGGATGCTTCATGAGGTACTCAGACACTGACTTTCTTAACAAGGAAGTGGAAAATGGGAGATCAGGAGGTAATGTAGTAGTGATAGTCATTGCAGTAGTTAGTTCCATGATCGTTGCGGTGGTTGCTGTAACTTTTGGAGCTTATATCTGGAAACAGAGATACATTCAAAAGAAAAGAAGAGGATCAAATGATGCAGAAAAGTTAGCAAAGAGTTTAGAGCAAAAAAGCTTGAACTTCAAGTACTCTACATTGGAAAAAGCTACTGGATCTTTTAATGACAATAGCAAACTAGGGCATGGAGGATTCGGAACGGGAGTTCTACCTGATGGAAGAGAGATTGCTATAAAGAGGTTATATTTCAACAACAGACATAGAGCAGCAGATTTCTACAACGAAGTCGACATAATTAGCGGTGTCGAACACAAGAATCTAGTTAGACTATTAGGGTGCAGTTGTTCAGGACCTGAAAGCCTTCTTGTATATGAATTTATGCCTAACAGAAGTCTTGATCGCTTCATTTTTGATAAAAACAAGGGCAGAGAACTAAACTGGGAAAAAAGATATGAGATTATTATTGGGACAGCTGAAGGATTAGTTTACCTGCATGAGAACTCCAAAATCAGAATAATTCATAGAGATATAAAAGCCAGCAACATCCTATTGGATTCAAAGCTTCGAGCTAAAATTGCAGATTTTGGTTTGGCCAGGTCCTTTCAAGAAGACAAGAGCCACATAAGCACAGCTATTGCAGGAACTTTGGGGTATATGGCTCCAGAGTATCTAGCTCATGGTCAGTTAACAGAAAAGGCAGACGTATATAGTTTCGGAGTATTGCTGCTAGAGATAGTTACTGGAAGACAGAATAACAGGAGCAAAGCATCAGAATATTCAGACAGTTTAGTTATAGTGACATGGAAGCATTTTCAGTCGGGGACTGCCGAACAGTTGTTCGATCCAAATATAGAGATGCACAACAACCGCAATAGCGATGTTAAGAATGAGATTCTAAGAGTGATTCACATAGGACTTCTATGCATCCAAGAGGTCGCTTCATTGCGACCAACTATGTCAAAGGCGTTACAGATGCTAACCAAGAATGAAGAGCATCTTGTTGCTCCCTCTAATCCACCTTTCTTAGATGAGAGTACCATGGAGCTCCATGACACAAGTGGCGACCCATTTTACCTCCTCAAAACAGATGATTCAGTTGCTACTATGTCAAATAGCTCTTTCTATCCCAGGTAA

Protein sequence

>Msa0842650

MKKPVPLFFSQCLKLLLIKFALLSETIIAEPRAKTVMIICGHQVEHNTSIFVPNFVATMEKISEQMRSTGFGTAVTGTGPDDNYGLAQCYGDLSLLDCVLCYAEARTVLPQCFPYNSGRIYLDGCFMRSANYSFFNEYTGKEDRAVCGNTTKKSLGFQVAAKEAVLKAVRDAPKNKGYAREKVVVSGTANESLYVLADCWRSLDSKSCKACLEHASSSILRCLPSSEGRALNTGCFMRYSDTDFLNKEVENGRSGGNVVVIVIAVVSSMIVAVVAVTFGAYIWKQRYIQKKRRGSNDAEKLAKSLEQKSLNFKYSTLEKATGSFNDNSKLGHGGFGTGVLPDGREIAIKRLYFNNRHRAADFYNEVDIISGVEHKNLVRLLGCSCSGPESLLVYEFMPNRSLDRFIFDKNKGRELNWEKRYEIIIGTAEGLVYLHENSKIRIIHRDIKASNILLDSKLRAKIADFGLARSFQEDKSHISTAIAGTLGYMAPEYLAHGQLTEKADVYSFGVLLLEIVTGRQNNRSKASEYSDSLVIVTWKHFQSGTAEQLFDPNIEMHNNRNSDVKNEILRVIHIGLLCIQEVASLRPTMSKALQMLTKNEEHLVAPSNPPFLDESTMELHDTSGDPFYLLKTDDSVATMSNSSFYPR*