AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0545120

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 77 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTCTGATGTTGTGGTTAT+AGG 0.164970 4_1:+34881508 Msa0545120:intron
TCTTAAGCGTGAAGTGTTTA+AGG 0.168191 4_1:+34881139 Msa0545120:five_prime_UTR
AGGTGTTCGGCAGCTTGTTC+TGG 0.174611 4_1:-34883454 None:intergenic
AAGAACTTCTTCTCAAAGTA+TGG 0.233679 4_1:+34883427 Msa0545120:CDS
TGTTATTGATCAAGTTATTC+AGG 0.259959 4_1:+34882978 Msa0545120:CDS
TAAGGCGAAACCGGCGTTAT+TGG 0.276097 4_1:+34881157 Msa0545120:five_prime_UTR
AAGAAAAGGGTGAGAGAAAC+GGG 0.276838 4_1:+34880519 None:intergenic
AATAGAGTCACTCTGTTTCT+AGG 0.283537 4_1:+34881246 Msa0545120:five_prime_UTR
ATATGCCGTCCTCGAGGATT+CGG 0.289741 4_1:+34882931 Msa0545120:five_prime_UTR
ATTCGGGTTTATTACTTATA+CGG 0.293630 4_1:+34882948 Msa0545120:five_prime_UTR
TTGTTCTCATTTCTAATTAC+AGG 0.294038 4_1:-34883478 None:intergenic
TTTCTGATGTTGTGGTTATA+GGG 0.316404 4_1:+34881509 Msa0545120:intron
GTGAGAGAAACGGGAGAAGA+AGG 0.328058 4_1:+34880528 Msa0545120:five_prime_UTR
TGCAAAATGTCCGCTCCTTT+TGG 0.330249 4_1:-34881353 None:intergenic
TATTTCTCTCATCGTCATTA+TGG 0.332643 4_1:-34882691 None:intergenic
CTTCACGCTTAAGAAGCTTC+TGG 0.361296 4_1:-34881130 None:intergenic
TGCTGAAGCAAAGAGTAATT+AGG 0.363146 4_1:+34883648 Msa0545120:three_prime_UTR
AAGACTCCATGGTTTCTAAC+AGG 0.367139 4_1:+34881548 Msa0545120:five_prime_UTR
CTCCGGTTGCTCTATTTCTC+AGG 0.376825 4_1:+34880599 Msa0545120:five_prime_UTR
TGAGAGAAACGGGAGAAGAA+GGG 0.384248 4_1:+34880529 Msa0545120:five_prime_UTR
GGTGTTCGGCAGCTTGTTCT+GGG 0.389840 4_1:-34883453 None:intergenic
TATGCCGTCCTCGAGGATTC+GGG 0.392313 4_1:+34882932 Msa0545120:five_prime_UTR
AAAGAAAAGGGTGAGAGAAA+CGG 0.402108 4_1:+34880518 None:intergenic
TCAGTATTGGTGTTATATCA+AGG 0.416664 4_1:+34881273 Msa0545120:five_prime_UTR
TGGTGTTATATCAAGGGTTA+AGG 0.419785 4_1:+34881280 Msa0545120:five_prime_UTR
AACGCGAACGGGAACGGAAA+GGG 0.425242 4_1:+34880554 Msa0545120:five_prime_UTR
ACCCTTTGTAATAGTCCTCT+TGG 0.426530 4_1:-34883149 None:intergenic
CTCGTCAAATAATTGAAACT+TGG 0.440566 4_1:+34881211 Msa0545120:five_prime_UTR
TCATTATGGAGTGAACTTGC+TGG 0.457097 4_1:-34882677 None:intergenic
GTTTCTAGGACTGTCAGTAT+TGG 0.459566 4_1:+34881260 Msa0545120:five_prime_UTR
AACCTGAGAAATAGAGCAAC+CGG 0.465083 4_1:-34880601 None:intergenic
AAACCTTCAGATACAGTTGC+TGG 0.469643 4_1:-34883223 None:intergenic
GAGAGAAACGGGAGAAGAAG+GGG 0.475313 4_1:+34880530 Msa0545120:five_prime_UTR
TGTTGATGGTCGAGATAGAA+AGG 0.475552 4_1:+34882725 Msa0545120:five_prime_UTR
TGAAGTGTTTAAGGCGAAAC+CGG 0.479324 4_1:+34881148 Msa0545120:five_prime_UTR
TCTAATTACAGGAAGGTGTT+CGG 0.480701 4_1:-34883467 None:intergenic
AACCAAGAGGACTATTACAA+AGG 0.488658 4_1:+34883147 Msa0545120:CDS
TTTAACACTTTCCTGCCAAA+AGG 0.494159 4_1:+34881338 Msa0545120:five_prime_UTR
TTCTGATGTTGTGGTTATAG+GGG 0.497751 4_1:+34881510 Msa0545120:intron
TTAAGAAGCTTCTGGAACTT+CGG 0.499408 4_1:-34881122 None:intergenic
ATAGCGCGTGGAGTTGTTGA+TGG 0.504153 4_1:+34882711 Msa0545120:five_prime_UTR
AAATATTCAAAGTGAAGGGA+GGG 0.506185 4_1:+34883571 Msa0545120:three_prime_UTR
ACCAAGAGGACTATTACAAA+GGG 0.508916 4_1:+34883148 Msa0545120:CDS
GAACGCGAACGGGAACGGAA+AGG 0.515756 4_1:+34880553 Msa0545120:five_prime_UTR
AGAACTTCTTCTCAAAGTAT+GGG 0.527076 4_1:+34883428 Msa0545120:CDS
AGAGACGATGGTGTTGATGC+AGG 0.529064 4_1:-34880759 None:intergenic
CTGGACATGAGCTATGGCTA+TGG 0.534932 4_1:-34880783 None:intergenic
TGCTCCACTTGAAGACTCCA+TGG 0.538936 4_1:+34881537 Msa0545120:five_prime_UTR
AGAAATAGAGCAACCGGAGT+CGG 0.547780 4_1:-34880595 None:intergenic
GAGACGATGGTGTTGATGCA+GGG 0.553931 4_1:-34880758 None:intergenic
CACTTTCCTGCCAAAAGGAG+CGG 0.563203 4_1:+34881343 Msa0545120:five_prime_UTR
AAAATATTCAAAGTGAAGGG+AGG 0.563975 4_1:+34883570 Msa0545120:three_prime_UTR
AAATACCTGGACATGAGCTA+TGG 0.566461 4_1:-34880789 None:intergenic
CATAGCCATAGCTCATGTCC+AGG 0.566882 4_1:+34880784 Msa0545120:five_prime_UTR
AAGCTACCTGTTAGAAACCA+TGG 0.567245 4_1:-34881554 None:intergenic
TCACATCATCAATGACAAGA+AGG 0.569815 4_1:+34883005 Msa0545120:CDS
CAGTATTGGTGTTATATCAA+GGG 0.571683 4_1:+34881274 Msa0545120:five_prime_UTR
AATATTCAAAGTGAAGGGAG+GGG 0.573065 4_1:+34883572 Msa0545120:three_prime_UTR
CTTATCAACGCCAATAACGC+CGG 0.573951 4_1:-34881167 None:intergenic
ATTCCAGCAACTGTATCTGA+AGG 0.581606 4_1:+34883220 Msa0545120:CDS
TGGACATGAGCTATGGCTAT+GGG 0.583402 4_1:-34880782 None:intergenic
ATAAGACGTGTAGTTGAAGC+TGG 0.589863 4_1:+34881185 Msa0545120:five_prime_UTR
TCTCATTTCTAATTACAGGA+AGG 0.596659 4_1:-34883474 None:intergenic
AGGGTTAAGGAACTATTCAA+AGG 0.610387 4_1:+34881293 Msa0545120:five_prime_UTR
GCGAACGGGAACGGAAAGGG+AGG 0.615429 4_1:+34880557 Msa0545120:five_prime_UTR
AATGAGAACAAGTCGACATG+AGG 0.619913 4_1:+34883490 Msa0545120:three_prime_UTR
AGAGAAACGGGAGAAGAAGG+GGG 0.620332 4_1:+34880531 Msa0545120:five_prime_UTR
TATGGCTATGGGAGAGACGA+TGG 0.629432 4_1:-34880771 None:intergenic
GAACTTCTTCTCAAAGTATG+GGG 0.639196 4_1:+34883429 Msa0545120:CDS
TAAACCCGAATCCTCGAGGA+CGG 0.641355 4_1:-34882936 None:intergenic
GAAACCATGGAGTCTTCAAG+TGG 0.642401 4_1:-34881541 None:intergenic
GTAATAAACCCGAATCCTCG+AGG 0.647085 4_1:-34882940 None:intergenic
CATATCAGGTTGAAACCAAG+AGG 0.664183 4_1:+34883134 Msa0545120:intron
AGACGTGTAGTTGAAGCTGG+TGG 0.676996 4_1:+34881188 Msa0545120:five_prime_UTR
ACGATGAGAGAAATAGCGCG+TGG 0.694923 4_1:+34882699 Msa0545120:five_prime_UTR
GATAGTCTGCTACATTACCA+TGG 0.696938 4_1:+34882889 Msa0545120:five_prime_UTR
TGATTGATATGCCGTCCTCG+AGG 0.702496 4_1:+34882925 Msa0545120:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTTACAGAATTTTTT+TGG + chr4_1:34880810-34880829 Msa0545120:intron 10.0%
!!! TATTTTTACAGAATTTTTTT+GGG + chr4_1:34880811-34880830 Msa0545120:intron 10.0%
!! TATAATGAATTAATAGTTTA+AGG + chr4_1:34881900-34881919 Msa0545120:intron 10.0%
!! ATAATGAATTAATAGTTTAA+GGG + chr4_1:34881901-34881920 Msa0545120:intron 10.0%
!!! ATATTTTTTATCTAAATTTC+TGG + chr4_1:34881962-34881981 Msa0545120:intron 10.0%
!!! TTATATGAAAATTTTGAAAT+TGG + chr4_1:34882309-34882328 Msa0545120:intron 10.0%
!!! AGTTTCTTTTATTTTAATTG+AGG + chr4_1:34880948-34880967 Msa0545120:intron 15.0%
!!! GTTTCTTTTATTTTAATTGA+GGG + chr4_1:34880949-34880968 Msa0545120:intron 15.0%
!! AACAAATCATAAATAACAAA+TGG - chr4_1:34881393-34881412 None:intergenic 15.0%
!!! TAGATTTTTGTTTAAGATAT+TGG + chr4_1:34881596-34881615 Msa0545120:intron 15.0%
!! TAATGAATTAATAGTTTAAG+GGG + chr4_1:34881902-34881921 Msa0545120:intron 15.0%
!!! ATTTGCATATTTTTTAAATG+AGG + chr4_1:34881930-34881949 Msa0545120:intron 15.0%
!! CAGAAAATTTATGATATAAA+TGG + chr4_1:34882198-34882217 Msa0545120:intron 15.0%
!! AAAAGGTAGATTTATATATA+TGG - chr4_1:34882534-34882553 None:intergenic 15.0%
!! TCAATACATCAAATTAAAAA+AGG - chr4_1:34882551-34882570 None:intergenic 15.0%
!!! ATTCAGTTTAATTTTTGATT+TGG + chr4_1:34883065-34883084 Msa0545120:intron 15.0%
!!! TTCAGTTTAATTTTTGATTT+GGG + chr4_1:34883066-34883085 Msa0545120:intron 15.0%
!! TTAAAAAATATTCAAAGTGA+AGG + chr4_1:34883566-34883585 Msa0545120:three_prime_UTR 15.0%
!! TAAAAAATATTCAAAGTGAA+GGG + chr4_1:34883567-34883586 Msa0545120:three_prime_UTR 15.0%
!! ATTACAAAAAACTAACTAAT+AGG - chr4_1:34883601-34883620 None:intergenic 15.0%
!!! TATTAGTTAGTTTTTTGTAA+TGG + chr4_1:34883600-34883619 Msa0545120:three_prime_UTR 15.0%
!!! TTTTGTTTATTTTGTTGAGT+TGG + chr4_1:34880711-34880730 Msa0545120:intron 20.0%
!! ATTCTGTAAAAATAAATACC+TGG - chr4_1:34880805-34880824 None:intergenic 20.0%
!!! TTTATGATAGACAAAGTTTT+TGG + chr4_1:34880875-34880894 Msa0545120:intron 20.0%
!!! TTGGATCATATCAAATTTTT+TGG + chr4_1:34880894-34880913 Msa0545120:intron 20.0%
!!! TAATTGAGGGTTTAATTTTT+TGG + chr4_1:34880962-34880981 Msa0545120:intron 20.0%
!!! GAACTTTTATATTTCATTTC+TGG - chr4_1:34881000-34881019 None:intergenic 20.0%
!! AATGGAAAAATTTCAGAAAA+TGG - chr4_1:34881022-34881041 None:intergenic 20.0%
!! TGTTAAATCCAAAAATCAAA+TGG - chr4_1:34881325-34881344 None:intergenic 20.0%
!!! AACAAAAATCTAAAATACCA+AGG - chr4_1:34881588-34881607 None:intergenic 20.0%
!! CAATAATTTATAGAGAAACA+TGG - chr4_1:34881693-34881712 None:intergenic 20.0%
!! CATGTTTCTCTATAAATTAT+TGG + chr4_1:34881691-34881710 Msa0545120:intron 20.0%
!! ATATAATGAGATCATTAGAT+AGG - chr4_1:34881867-34881886 None:intergenic 20.0%
!! AATGAATTAATAGTTTAAGG+GGG + chr4_1:34881903-34881922 Msa0545120:intron 20.0%
!!! CATAAATTTTCTGTATCATT+GGG - chr4_1:34882191-34882210 None:intergenic 20.0%
!!! TCATAAATTTTCTGTATCAT+TGG - chr4_1:34882192-34882211 None:intergenic 20.0%
!!! GAAAATTTTGAAATTGGTTT+TGG + chr4_1:34882315-34882334 Msa0545120:intron 20.0%
!! AAATAACACTCAATTAATGA+CGG - chr4_1:34882819-34882838 None:intergenic 20.0%
!!! GAGTGTTATTTACATTTTAT+AGG + chr4_1:34882828-34882847 Msa0545120:intron 20.0%
!!! AAAACTAAGAAGATTTTTGT+TGG + chr4_1:34883193-34883212 Msa0545120:CDS 20.0%
!!! TACAGAATTTTTTTGGGTTT+AGG + chr4_1:34880817-34880836 Msa0545120:intron 25.0%
!!! AGGGTTTAATTTTTTGGTTT+TGG + chr4_1:34880968-34880987 Msa0545120:intron 25.0%
!!! GGGTTTAATTTTTTGGTTTT+GGG + chr4_1:34880969-34880988 Msa0545120:intron 25.0%
! GAAGGAGGTAATAATAAAAA+TGG - chr4_1:34881040-34881059 None:intergenic 25.0%
! CTACTATGCATAATCTTATT+TGG + chr4_1:34882052-34882071 Msa0545120:intron 25.0%
!!! TTTTGAAATTGGTTTTGGAT+TGG + chr4_1:34882320-34882339 Msa0545120:intron 25.0%
!!! TTTTTGGTCATTAGTGTTTT+TGG + chr4_1:34882430-34882449 Msa0545120:intron 25.0%
! ATGTACTGCATTTATTTGAA+AGG + chr4_1:34882481-34882500 Msa0545120:intron 25.0%
! TGTACTGCATTTATTTGAAA+GGG + chr4_1:34882482-34882501 Msa0545120:intron 25.0%
!!! TTTGCCGTTTTTTTTAGTTA+TGG + chr4_1:34882604-34882623 Msa0545120:intron 25.0%
! TGCTCCATAACTAAAAAAAA+CGG - chr4_1:34882611-34882630 None:intergenic 25.0%
!! ATTCGGGTTTATTACTTATA+CGG + chr4_1:34882948-34882967 Msa0545120:five_prime_UTR 25.0%
! TGTTATTGATCAAGTTATTC+AGG + chr4_1:34882978-34882997 Msa0545120:CDS 25.0%
! AAGAAGGTAAAACTGTAATT+AGG + chr4_1:34883021-34883040 Msa0545120:intron 25.0%
! AGAAGGTAAAACTGTAATTA+GGG + chr4_1:34883022-34883041 Msa0545120:intron 25.0%
!!! TTGATTTGTTTGTTCATATC+AGG + chr4_1:34883120-34883139 Msa0545120:intron 25.0%
! GTTTGTATTGTATGATGATA+CGG + chr4_1:34883345-34883364 Msa0545120:intron 25.0%
! TTGTTCTCATTTCTAATTAC+AGG - chr4_1:34883481-34883500 None:intergenic 25.0%
!!! TAGTTAGTTTTTTGTAATGG+AGG + chr4_1:34883603-34883622 Msa0545120:three_prime_UTR 25.0%
TGTTCTTTGTGAGATGTTAA+TGG + chr4_1:34880683-34880702 Msa0545120:intron 30.0%
GTTCTTTGTGAGATGTTAAT+GGG + chr4_1:34880684-34880703 Msa0545120:intron 30.0%
TCCTTCAAAATTCACAATTG+CGG + chr4_1:34881054-34881073 Msa0545120:intron 30.0%
CTCGTCAAATAATTGAAACT+TGG + chr4_1:34881211-34881230 Msa0545120:five_prime_UTR 30.0%
!! TCAGTATTGGTGTTATATCA+AGG + chr4_1:34881273-34881292 Msa0545120:five_prime_UTR 30.0%
!! CAGTATTGGTGTTATATCAA+GGG + chr4_1:34881274-34881293 Msa0545120:five_prime_UTR 30.0%
TTTCTGATGTTGTGGTTATA+GGG + chr4_1:34881509-34881528 Msa0545120:intron 30.0%
TGGCATTAATTCAACTAGAA+TGG + chr4_1:34881616-34881635 Msa0545120:intron 30.0%
! TCCTTTTCGCATATTTACTT+TGG + chr4_1:34881757-34881776 Msa0545120:intron 30.0%
TCCAAAGTAAATATGCGAAA+AGG - chr4_1:34881761-34881780 None:intergenic 30.0%
AGATTACTGAACTGATAACA+AGG + chr4_1:34881999-34882018 Msa0545120:intron 30.0%
! CATACAACTCTTTCCAATTT+TGG - chr4_1:34882091-34882110 None:intergenic 30.0%
TGAAAAAGAAAGCAAGTTTC+TGG - chr4_1:34882225-34882244 None:intergenic 30.0%
TGTGTCCAAAATTCATGATA+TGG + chr4_1:34882250-34882269 Msa0545120:intron 30.0%
! CCTTTCCATATCATGAATTT+TGG - chr4_1:34882258-34882277 None:intergenic 30.0%
CCAAAATTCATGATATGGAA+AGG + chr4_1:34882255-34882274 Msa0545120:intron 30.0%
ATCAAAGAGCTTTCTATATC+AGG - chr4_1:34882290-34882309 None:intergenic 30.0%
!!! ATTGGTTTTGGATTGGATTA+TGG + chr4_1:34882327-34882346 Msa0545120:intron 30.0%
!!! AGCGATGAGTGTTTTATTTT+TGG + chr4_1:34882414-34882433 Msa0545120:intron 30.0%
AAGACATTGAATTGAATTGC+TGG - chr4_1:34882648-34882667 None:intergenic 30.0%
TATTTCTCTCATCGTCATTA+TGG - chr4_1:34882694-34882713 None:intergenic 30.0%
! TACACAATTTTCGTGCTTAT+TGG + chr4_1:34882788-34882807 Msa0545120:intron 30.0%
! ACTAAGAAGATTTTTGTTGG+TGG + chr4_1:34883196-34883215 Msa0545120:CDS 30.0%
AAGAACTTCTTCTCAAAGTA+TGG + chr4_1:34883427-34883446 Msa0545120:CDS 30.0%
AGAACTTCTTCTCAAAGTAT+GGG + chr4_1:34883428-34883447 Msa0545120:CDS 30.0%
TCTCATTTCTAATTACAGGA+AGG - chr4_1:34883477-34883496 None:intergenic 30.0%
AAAATATTCAAAGTGAAGGG+AGG + chr4_1:34883570-34883589 Msa0545120:three_prime_UTR 30.0%
AAATATTCAAAGTGAAGGGA+GGG + chr4_1:34883571-34883590 Msa0545120:three_prime_UTR 30.0%
! ATGATGATGATGAACAGAAC+AGG - chr4_1:34880632-34880651 None:intergenic 35.0%
! AATTTTGCTTGCATGCATTC+AGG + chr4_1:34880844-34880863 Msa0545120:intron 35.0%
!! GCAATTGTGAATTTTGAAGG+AGG - chr4_1:34881055-34881074 None:intergenic 35.0%
! ACCGCAATTGTGAATTTTGA+AGG - chr4_1:34881058-34881077 None:intergenic 35.0%
AAGAATTTCGCTAATCTAGC+AGG - chr4_1:34881101-34881120 None:intergenic 35.0%
!! TTAAGAAGCTTCTGGAACTT+CGG - chr4_1:34881125-34881144 None:intergenic 35.0%
! TCTTAAGCGTGAAGTGTTTA+AGG + chr4_1:34881139-34881158 Msa0545120:five_prime_UTR 35.0%
! AATAGAGTCACTCTGTTTCT+AGG + chr4_1:34881246-34881265 Msa0545120:five_prime_UTR 35.0%
!! TGGTGTTATATCAAGGGTTA+AGG + chr4_1:34881280-34881299 Msa0545120:five_prime_UTR 35.0%
AGGGTTAAGGAACTATTCAA+AGG + chr4_1:34881293-34881312 Msa0545120:five_prime_UTR 35.0%
!! GGTCACAACCATTTGATTTT+TGG + chr4_1:34881314-34881333 Msa0545120:five_prime_UTR 35.0%
TTTAACACTTTCCTGCCAAA+AGG + chr4_1:34881338-34881357 Msa0545120:five_prime_UTR 35.0%
! GACATTTTGCATCAAACAGA+AGG + chr4_1:34881365-34881384 Msa0545120:five_prime_UTR 35.0%
ACATGTACTTTCTGATGTTG+TGG + chr4_1:34881501-34881520 Msa0545120:intron 35.0%
CTTTCTGATGTTGTGGTTAT+AGG + chr4_1:34881508-34881527 Msa0545120:intron 35.0%
TTCTGATGTTGTGGTTATAG+GGG + chr4_1:34881510-34881529 Msa0545120:intron 35.0%
GAAACATGGAAGAAGAAAGA+AGG - chr4_1:34881679-34881698 None:intergenic 35.0%
CGCATATTTACTTTGGATTG+AGG + chr4_1:34881764-34881783 Msa0545120:intron 35.0%
! ACTTTGGATTGAGGTTGATT+CGG + chr4_1:34881773-34881792 Msa0545120:intron 35.0%
!! ATTGTTGCGCTTTGAAACTA+TGG + chr4_1:34881834-34881853 Msa0545120:intron 35.0%
!! TCTTATTTGGTGCCACATTT+AGG + chr4_1:34882065-34882084 Msa0545120:intron 35.0%
!! TTCCAATTTTGGCCTAAATG+TGG - chr4_1:34882080-34882099 None:intergenic 35.0%
TATTGATGTATCCCAAAGCT+AGG - chr4_1:34882353-34882372 None:intergenic 35.0%
! TTCTTTGTTGTGATTCAAGC+AGG + chr4_1:34882867-34882886 Msa0545120:intron 35.0%
!! ATCAGTTTGCATTTTGACCA+TGG - chr4_1:34882909-34882928 None:intergenic 35.0%
TCACATCATCAATGACAAGA+AGG + chr4_1:34883005-34883024 Msa0545120:CDS 35.0%
AACCAAGAGGACTATTACAA+AGG + chr4_1:34883147-34883166 Msa0545120:CDS 35.0%
ACCAAGAGGACTATTACAAA+GGG + chr4_1:34883148-34883167 Msa0545120:CDS 35.0%
GAACTTCTTCTCAAAGTATG+GGG + chr4_1:34883429-34883448 Msa0545120:CDS 35.0%
!! TCTAATTACAGGAAGGTGTT+CGG - chr4_1:34883470-34883489 None:intergenic 35.0%
AATATTCAAAGTGAAGGGAG+GGG + chr4_1:34883572-34883591 Msa0545120:three_prime_UTR 35.0%
TGCTGAAGCAAAGAGTAATT+AGG + chr4_1:34883648-34883667 Msa0545120:three_prime_UTR 35.0%
AACCTGAGAAATAGAGCAAC+CGG - chr4_1:34880604-34880623 None:intergenic 40.0%
! GTTGCTCTATTTCTCAGGTT+CGG + chr4_1:34880604-34880623 Msa0545120:intron 40.0%
AAATACCTGGACATGAGCTA+TGG - chr4_1:34880792-34880811 None:intergenic 40.0%
! TGAAGTGTTTAAGGCGAAAC+CGG + chr4_1:34881148-34881167 Msa0545120:five_prime_UTR 40.0%
ATAAGACGTGTAGTTGAAGC+TGG + chr4_1:34881185-34881204 Msa0545120:five_prime_UTR 40.0%
!! GTTTCTAGGACTGTCAGTAT+TGG + chr4_1:34881260-34881279 Msa0545120:five_prime_UTR 40.0%
AAGACTCCATGGTTTCTAAC+AGG + chr4_1:34881548-34881567 Msa0545120:five_prime_UTR 40.0%
AAGCTACCTGTTAGAAACCA+TGG - chr4_1:34881557-34881576 None:intergenic 40.0%
! AGGTAGCTTATGCTTTTCCT+TGG + chr4_1:34881568-34881587 Msa0545120:intron 40.0%
ATGGAAGAAGAAAGAAGGAG+AGG - chr4_1:34881674-34881693 None:intergenic 40.0%
!! TGCGCTTTGAAACTATGGTT+TGG + chr4_1:34881839-34881858 Msa0545120:intron 40.0%
!! GCGCTTTGAAACTATGGTTT+GGG + chr4_1:34881840-34881859 Msa0545120:intron 40.0%
GATAACAAGGCTGATCATGA+TGG + chr4_1:34882012-34882031 Msa0545120:intron 40.0%
TGCCACATTTAGGCCAAAAT+TGG + chr4_1:34882075-34882094 Msa0545120:intron 40.0%
ATTGGATTATGGCCTAGCTT+TGG + chr4_1:34882338-34882357 Msa0545120:intron 40.0%
! TTGGATTATGGCCTAGCTTT+GGG + chr4_1:34882339-34882358 Msa0545120:intron 40.0%
TCATTATGGAGTGAACTTGC+TGG - chr4_1:34882680-34882699 None:intergenic 40.0%
!! TGTTGATGGTCGAGATAGAA+AGG + chr4_1:34882725-34882744 Msa0545120:five_prime_UTR 40.0%
GATAGTCTGCTACATTACCA+TGG + chr4_1:34882889-34882908 Msa0545120:five_prime_UTR 40.0%
!! CATATCAGGTTGAAACCAAG+AGG + chr4_1:34883134-34883153 Msa0545120:intron 40.0%
ACCCTTTGTAATAGTCCTCT+TGG - chr4_1:34883152-34883171 None:intergenic 40.0%
ATTCCAGCAACTGTATCTGA+AGG + chr4_1:34883220-34883239 Msa0545120:CDS 40.0%
AAACCTTCAGATACAGTTGC+TGG - chr4_1:34883226-34883245 None:intergenic 40.0%
AGCTTATTGTATGCTTGCCT+TGG + chr4_1:34883383-34883402 Msa0545120:intron 40.0%
GCTTATTGTATGCTTGCCTT+GGG + chr4_1:34883384-34883403 Msa0545120:intron 40.0%
! TCATCTGAAACACAAACCCA+AGG - chr4_1:34883403-34883422 None:intergenic 40.0%
AATGAGAACAAGTCGACATG+AGG + chr4_1:34883490-34883509 Msa0545120:three_prime_UTR 40.0%
TGAGAGAAACGGGAGAAGAA+GGG + chr4_1:34880529-34880548 Msa0545120:five_prime_UTR 45.0%
ACGGAAAGGGAGGAGAAAAA+CGG + chr4_1:34880567-34880586 Msa0545120:five_prime_UTR 45.0%
AGAAATAGAGCAACCGGAGT+CGG - chr4_1:34880598-34880617 None:intergenic 45.0%
TGGACATGAGCTATGGCTAT+GGG - chr4_1:34880785-34880804 None:intergenic 45.0%
GCGGTGTTCGATTGAATTTC+CGG + chr4_1:34881073-34881092 Msa0545120:intron 45.0%
TTCGCTAATCTAGCAGGTTC+CGG - chr4_1:34881095-34881114 None:intergenic 45.0%
CTTCACGCTTAAGAAGCTTC+TGG - chr4_1:34881133-34881152 None:intergenic 45.0%
CTTATCAACGCCAATAACGC+CGG - chr4_1:34881170-34881189 None:intergenic 45.0%
! TGCAAAATGTCCGCTCCTTT+TGG - chr4_1:34881356-34881375 None:intergenic 45.0%
GAAACCATGGAGTCTTCAAG+TGG - chr4_1:34881544-34881563 None:intergenic 45.0%
AGGAGAGGAAATGACAGATG+TGG - chr4_1:34881659-34881678 None:intergenic 45.0%
GTAATAAACCCGAATCCTCG+AGG - chr4_1:34882943-34882962 None:intergenic 45.0%
GTGAGAGAAACGGGAGAAGA+AGG + chr4_1:34880528-34880547 Msa0545120:five_prime_UTR 50.0%
GAGAGAAACGGGAGAAGAAG+GGG + chr4_1:34880530-34880549 Msa0545120:five_prime_UTR 50.0%
AGAGAAACGGGAGAAGAAGG+GGG + chr4_1:34880531-34880550 Msa0545120:five_prime_UTR 50.0%
GAAAGGGAGGAGAAAAACGG+CGG + chr4_1:34880570-34880589 Msa0545120:five_prime_UTR 50.0%
! CTCCGGTTGCTCTATTTCTC+AGG + chr4_1:34880599-34880618 Msa0545120:five_prime_UTR 50.0%
!! GAGACGATGGTGTTGATGCA+GGG - chr4_1:34880761-34880780 None:intergenic 50.0%
!! AGAGACGATGGTGTTGATGC+AGG - chr4_1:34880762-34880781 None:intergenic 50.0%
TATGGCTATGGGAGAGACGA+TGG - chr4_1:34880774-34880793 None:intergenic 50.0%
CTGGACATGAGCTATGGCTA+TGG - chr4_1:34880786-34880805 None:intergenic 50.0%
CATAGCCATAGCTCATGTCC+AGG + chr4_1:34880784-34880803 Msa0545120:five_prime_UTR 50.0%
TAAGGCGAAACCGGCGTTAT+TGG + chr4_1:34881157-34881176 Msa0545120:five_prime_UTR 50.0%
AGACGTGTAGTTGAAGCTGG+TGG + chr4_1:34881188-34881207 Msa0545120:five_prime_UTR 50.0%
CACTTTCCTGCCAAAAGGAG+CGG + chr4_1:34881343-34881362 Msa0545120:five_prime_UTR 50.0%
! AAATGTCCGCTCCTTTTGGC+AGG - chr4_1:34881352-34881371 None:intergenic 50.0%
TGCTCCACTTGAAGACTCCA+TGG + chr4_1:34881537-34881556 Msa0545120:five_prime_UTR 50.0%
ACGATGAGAGAAATAGCGCG+TGG + chr4_1:34882699-34882718 Msa0545120:five_prime_UTR 50.0%
ATAGCGCGTGGAGTTGTTGA+TGG + chr4_1:34882711-34882730 Msa0545120:five_prime_UTR 50.0%
TGATTGATATGCCGTCCTCG+AGG + chr4_1:34882925-34882944 Msa0545120:five_prime_UTR 50.0%
ATATGCCGTCCTCGAGGATT+CGG + chr4_1:34882931-34882950 Msa0545120:five_prime_UTR 50.0%
TAAACCCGAATCCTCGAGGA+CGG - chr4_1:34882939-34882958 None:intergenic 50.0%
AGAAGAAGGGGGAACGCGAA+CGG + chr4_1:34880542-34880561 Msa0545120:five_prime_UTR 55.0%
AACGCGAACGGGAACGGAAA+GGG + chr4_1:34880554-34880573 Msa0545120:five_prime_UTR 55.0%
TATGCCGTCCTCGAGGATTC+GGG + chr4_1:34882932-34882951 Msa0545120:five_prime_UTR 55.0%
! GGTGTTCGGCAGCTTGTTCT+GGG - chr4_1:34883456-34883475 None:intergenic 55.0%
! AGGTGTTCGGCAGCTTGTTC+TGG - chr4_1:34883457-34883476 None:intergenic 55.0%
GAAGAAGGGGGAACGCGAAC+GGG + chr4_1:34880543-34880562 Msa0545120:five_prime_UTR 60.0%
GAACGCGAACGGGAACGGAA+AGG + chr4_1:34880553-34880572 Msa0545120:five_prime_UTR 60.0%
AGGGGGAACGCGAACGGGAA+CGG + chr4_1:34880548-34880567 Msa0545120:five_prime_UTR 65.0%
GCGAACGGGAACGGAAAGGG+AGG + chr4_1:34880557-34880576 Msa0545120:five_prime_UTR 65.0%
!! AAAAACGGCGGCGCCGACTC+CGG + chr4_1:34880582-34880601 Msa0545120:five_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 34880523 34883682 34880523 ID=Msa0545120;Name=Msa0545120
chr4_1 mRNA 34880523 34883682 34880523 ID=Msa0545120-mRNA-1;Parent=Msa0545120;Name=Msa0545120-mRNA-1;_AED=0.07;_eAED=0.07;_QI=655|1|1|1|0.28|0.25|8|198|73
chr4_1 exon 34880523 34880620 34880523 ID=Msa0545120-mRNA-1:exon:8789;Parent=Msa0545120-mRNA-1
chr4_1 exon 34880730 34880805 34880730 ID=Msa0545120-mRNA-1:exon:8790;Parent=Msa0545120-mRNA-1
chr4_1 exon 34881111 34881386 34881111 ID=Msa0545120-mRNA-1:exon:8791;Parent=Msa0545120-mRNA-1
chr4_1 exon 34881521 34881569 34881521 ID=Msa0545120-mRNA-1:exon:8792;Parent=Msa0545120-mRNA-1
chr4_1 exon 34882673 34882746 34882673 ID=Msa0545120-mRNA-1:exon:8793;Parent=Msa0545120-mRNA-1
chr4_1 exon 34882889 34883026 34882889 ID=Msa0545120-mRNA-1:exon:8794;Parent=Msa0545120-mRNA-1
chr4_1 exon 34883142 34883241 34883142 ID=Msa0545120-mRNA-1:exon:8795;Parent=Msa0545120-mRNA-1
chr4_1 exon 34883419 34883682 34883419 ID=Msa0545120-mRNA-1:exon:8796;Parent=Msa0545120-mRNA-1
chr4_1 five_prime_UTR 34880523 34880620 34880523 ID=Msa0545120-mRNA-1:five_prime_utr;Parent=Msa0545120-mRNA-1
chr4_1 five_prime_UTR 34880730 34880805 34880730 ID=Msa0545120-mRNA-1:five_prime_utr;Parent=Msa0545120-mRNA-1
chr4_1 five_prime_UTR 34881111 34881386 34881111 ID=Msa0545120-mRNA-1:five_prime_utr;Parent=Msa0545120-mRNA-1
chr4_1 five_prime_UTR 34881521 34881569 34881521 ID=Msa0545120-mRNA-1:five_prime_utr;Parent=Msa0545120-mRNA-1
chr4_1 five_prime_UTR 34882673 34882746 34882673 ID=Msa0545120-mRNA-1:five_prime_utr;Parent=Msa0545120-mRNA-1
chr4_1 five_prime_UTR 34882889 34882970 34882889 ID=Msa0545120-mRNA-1:five_prime_utr;Parent=Msa0545120-mRNA-1
chr4_1 CDS 34882971 34883026 34882971 ID=Msa0545120-mRNA-1:cds;Parent=Msa0545120-mRNA-1
chr4_1 CDS 34883142 34883241 34883142 ID=Msa0545120-mRNA-1:cds;Parent=Msa0545120-mRNA-1
chr4_1 CDS 34883419 34883484 34883419 ID=Msa0545120-mRNA-1:cds;Parent=Msa0545120-mRNA-1
chr4_1 three_prime_UTR 34883485 34883682 34883485 ID=Msa0545120-mRNA-1:three_prime_utr;Parent=Msa0545120-mRNA-1
Gene Sequence

>Msa0545120

ATGCTTCTGTTATTGATCAAGTTATTCAGGAGAATCACATCATCAATGACAAGAAGGTTGAAACCAAGAGGACTATTACAAAGGGTGCATCTCAAACTAATGACTTTAAAACTAAGAAGATTTTTGTTGGTGGCATTCCAGCAACTGTATCTGAAGATGAGCTCAAGAACTTCTTCTCAAAGTATGGGGACCCAGAACAAGCTGCCGAACACCTTCCTGTAA
Protein sequence

>Msa0545120

MLLLLIKLFRRITSSMTRRLKPRGLLQRVHLKLMTLKLRRFLLVAFQQLYLKMSSRTSSQSMGTQNKLPNTFL*