AlfalfaGEDB | Msa1288550

M. sativa cultivar zhongmu-4 / Msa1288550

KEGG: K00975
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0006073 GO:0008150 GO:0008152 GO:0009501 GO:0009507 GO:0009536 GO:0009987 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0071704
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1288550	sp\|P52416\|GLGS1_VICFA	92.308	91	7	0	1	91	380	470	1.26e-50	171

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1288550	A0A2Z6NNT9	93.407	91	6	0	1	91	305	395	2.35e-49	172

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0011170	Msa1288550	0.800080	1.704329e-48	-8.615850e-47
Msa0019150	Msa1288550	0.805668	1.194754e-49	-8.615850e-47
Msa0100090	Msa1288550	0.805958	1.037970e-49	-8.615850e-47
Msa0211110	Msa1288550	0.821476	3.957828e-53	-8.615850e-47
Msa0229990	Msa1288550	0.825036	5.835973e-54	-8.615850e-47
Msa0267150	Msa1288550	0.857002	2.195529e-62	-8.615850e-47
Msa0273100	Msa1288550	0.805530	1.276709e-49	-8.615850e-47
Msa0281030	Msa1288550	0.912898	1.148804e-83	-8.615850e-47
Msa0388850	Msa1288550	0.909359	6.213825e-82	-8.615850e-47
Msa0389320	Msa1288550	0.830539	2.773652e-55	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1288550	MtrunA17_Chr5g0447341	94.505	91	5	0	1	91	387	477	6.76e-53	174
Msa1288550	MtrunA17_Chr3g0120471	82.418	91	13	1	1	91	386	473	3.06e-44	151
Msa1288550	MtrunA17_Chr3g0144731	48.235	85	44	0	6	90	38	122	2.49e-25	94.4
Msa1288550	MtrunA17_Chr7g0271011	47.674	86	45	0	5	90	412	497	7.77e-25	98.6
Msa1288550	MtrunA17_Chr3g0141761	45.238	84	46	0	6	89	407	490	3.96e-23	93.6
Msa1288550	MtrunA17_Chr4g0074531	50.000	88	44	0	3	90	395	482	1.94e-22	91.7
Msa1288550	MtrunA17_Chr4g0035171	45.882	85	46	0	6	90	405	489	3.93e-22	90.9
Msa1288550	MtrunA17_Chr6g0454161	74.545	55	14	0	33	87	121	175	2.97e-21	87.4
Msa1288550	MtrunA17_Chr4g0077071	92.500	40	3	0	1	40	42	81	1.20e-19	77.4
Msa1288550	MtrunA17_Chr4g0033371	91.176	34	3	0	92	125	421	454	2.70e-15	71.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1288550	AT5G48300.1	82.418	91	16	0	1	91	392	482	1.92e-47	160
Msa1288550	AT1G27680.1	46.739	92	48	1	6	96	395	486	2.78e-25	100
Msa1288550	AT5G19220.1	46.591	88	47	0	3	90	396	483	7.73e-23	93.2
Msa1288550	AT2G21590.2	43.529	85	48	0	6	90	400	484	1.35e-21	89.7
Msa1288550	AT2G21590.3	43.529	85	48	0	6	90	400	484	1.35e-21	89.7
Msa1288550	AT2G21590.5	43.529	85	48	0	6	90	400	484	1.35e-21	89.7
Msa1288550	AT2G21590.4	43.529	85	48	0	6	90	400	484	1.35e-21	89.7
Msa1288550	AT2G21590.7	43.529	85	48	0	6	90	400	484	1.35e-21	89.7
Msa1288550	AT2G21590.1	43.529	85	48	0	6	90	400	484	1.35e-21	89.7
Msa1288550	AT2G21590.6	43.529	85	48	0	6	90	400	484	1.35e-21	89.7

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Find 41 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCTTCCATTCAGTGGT+TGG	0.473513	8_3:+41542508	Msa1288550:CDS
AAATCTTCCATTCAGTGGTT+GGG	0.441064	8_3:+41542509	Msa1288550:CDS
AAGGCAATGATGAAGACAAG+AGG	0.640920	8_3:+41543704	Msa1288550:three_prime_UTR
AATGCAGCCCAACCACTGAA+TGG	0.520984	8_3:-41542516	None:intergenic
AATGTTTAAAGACAAATCTA+CGG	0.528586	8_3:+41543787	Msa1288550:three_prime_UTR
AATTAATTTGTCTTCAAATT+TGG	0.102206	8_3:+41544128	Msa1288550:three_prime_UTR
ACTGAATTTCAAATTGCATG+TGG	0.539019	8_3:+41543836	Msa1288550:three_prime_UTR
ACTGATAGTGTTATCGGGGA+AGG	0.575346	8_3:+41542348	Msa1288550:CDS
AGTGTTCACATGAGAAGGCT+TGG	0.497606	8_3:+41543499	Msa1288550:CDS
ATATCACTGATAGTGTTATC+GGG	0.469841	8_3:+41542343	Msa1288550:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TATTGAAGACACTTTGTTGA+TGG	+	chr8_3:41542561-41542580	Msa1288550:CDS	30.0%
ATTGAAGACACTTTGTTGAT+GGG	+	chr8_3:41542562-41542581	Msa1288550:CDS	30.0%
AGAAAATCAATGTTGCAGTT+AGG	-	chr8_3:41542745-41542764	None:intergenic	30.0%
TAGAATTGGAGAAAATGTGA+AGG	+	chr8_3:41542872-41542891	Msa1288550:CDS	30.0%
TCAAGCTTAATATGAGAAGA+TGG	-	chr8_3:41542950-41542969	None:intergenic	30.0%
TCGCAGTTTATTATTAGTGT+TGG	+	chr8_3:41543207-41543226	Msa1288550:intron	30.0%
GAAGGAAATCAGGTTATATT+CGG	-	chr8_3:41543271-41543290	None:intergenic	30.0%
TATAACCTGATTTCCTTCAT+AGG	+	chr8_3:41543273-41543292	Msa1288550:intron	30.0%
ACTGAATTTCAAATTGCATG+TGG	+	chr8_3:41543836-41543855	Msa1288550:three_prime_UTR	30.0%
CTGAATTTCAAATTGCATGT+GGG	+	chr8_3:41543837-41543856	Msa1288550:three_prime_UTR	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	41542330	41544174	41542330	ID=Msa1288550;Name=Msa1288550
chr8_3	mRNA	41542330	41544174	41542330	ID=Msa1288550-mRNA-1;Parent=Msa1288550;Name=Msa1288550-mRNA-1;_AED=0.22;_eAED=0.22;_QI=0\|0.75\|0.6\|0.8\|0.75\|0.8\|5\|485\|136
chr8_3	exon	41542330	41542383	41542330	ID=Msa1288550-mRNA-1:exon:11374;Parent=Msa1288550-mRNA-1
chr8_3	exon	41542502	41542600	41542502	ID=Msa1288550-mRNA-1:exon:11375;Parent=Msa1288550-mRNA-1
chr8_3	exon	41542774	41542893	41542774	ID=Msa1288550-mRNA-1:exon:11376;Parent=Msa1288550-mRNA-1
chr8_3	exon	41543448	41543572	41543448	ID=Msa1288550-mRNA-1:exon:11377;Parent=Msa1288550-mRNA-1
chr8_3	exon	41543677	41544174	41543677	ID=Msa1288550-mRNA-1:exon:11378;Parent=Msa1288550-mRNA-1
chr8_3	CDS	41542330	41542383	41542330	ID=Msa1288550-mRNA-1:cds;Parent=Msa1288550-mRNA-1
chr8_3	CDS	41542502	41542600	41542502	ID=Msa1288550-mRNA-1:cds;Parent=Msa1288550-mRNA-1
chr8_3	CDS	41542774	41542893	41542774	ID=Msa1288550-mRNA-1:cds;Parent=Msa1288550-mRNA-1
chr8_3	CDS	41543448	41543572	41543448	ID=Msa1288550-mRNA-1:cds;Parent=Msa1288550-mRNA-1
chr8_3	CDS	41543677	41543689	41543677	ID=Msa1288550-mRNA-1:cds;Parent=Msa1288550-mRNA-1
chr8_3	three_prime_UTR	41543690	41544174	41543690	ID=Msa1288550-mRNA-1:three_prime_utr;Parent=Msa1288550-mRNA-1

Gene Sequence

>Msa1288550

ATGCTTGATGCTGATATCACTGATAGTGTTATCGGGGAAGGATGTGTGATTAAGAACTGCAAAATCTTCCATTCAGTGGTTGGGCTGCATTCTTGCATATCAGGAGGTGCGATTATTGAAGACACTTTGTTGATGGGCGCATATTATTACGAGACAGAAGCTGATAAAAGTTTTTTGGCTGCTAAAGGCAGTGTTCCACTTGGTATTGGCAGAAACTCCCGTATCAAAAGAGCAATTGTTGACAAGAATGCTAGAATTGGAGAAAATGTGAAGGCAGATTTTGATGGAAATGGCACCTTCAAATGTGAAGAGTTCATCACAATGAGTGTTCACATGAGAAGGCTTGGAAATGATGAGCATCTCACAAAAGCATTCCCAACGATGTCGACTTGGATAAGGATGGTCGAGTAA

Protein sequence

>Msa1288550

MLDADITDSVIGEGCVIKNCKIFHSVVGLHSCISGGAIIEDTLLMGAYYYETEADKSFLAAKGSVPLGIGRNSRIKRAIVDKNARIGENVKADFDGNGTFKCEEFITMSVHMRRLGNDEHLTKAFPTMSTWIRMVE*