AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0468050

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0468050 MtrunA17_Chr3g0122261 98.462 130 2 0 1 130 1 130 4.74e-90 256
Msa0468050 MtrunA17_Chr8g0377531 53.623 138 50 4 1 130 82 213 1.20e-41 137
Msa0468050 MtrunA17_Chr5g0434711 88.235 68 8 0 15 82 4 71 2.57e-37 121
Msa0468050 MtrunA17_Chr7g0224931 37.500 128 61 3 1 109 21 148 4.08e-17 72.8
Msa0468050 MtrunA17_Chr7g0224971 37.500 128 61 3 1 109 21 148 4.08e-17 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0468050 AT1G27290.2 58.741 143 45 2 1 130 1 142 5.53e-52 161
Msa0468050 AT1G27290.1 58.741 143 45 2 1 130 1 142 5.53e-52 161

Find 75 sgRNAs with CRISPR-Local

Find 274 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCTCTTTGCATTACAAAT+AGG 0.200673 3_3:+69563481 Msa0468050:three_prime_UTR
GGATCGGTCACGTTCTTTAT+TGG 0.225023 3_3:+69562504 Msa0468050:CDS
GAATGGATAAAGTTGAAAAT+TGG 0.229504 3_3:-69562149 None:intergenic
GTAACCTTGCATCTAATAAA+AGG 0.231552 3_3:-69563440 None:intergenic
ATATAACAATTTCCACATTA+TGG 0.259950 3_3:+69563406 Msa0468050:three_prime_UTR
CAATGTTTATGTTTATGTTT+AGG 0.295585 3_3:-69562244 None:intergenic
CTTTGATTTCTTTCTCCAAT+TGG 0.306057 3_3:-69563343 None:intergenic
GTGGGTTATTGGATTGATTA+TGG 0.317283 3_3:+69562542 Msa0468050:CDS
ATCTCTTTGCATTACAAATA+GGG 0.322428 3_3:+69563482 Msa0468050:three_prime_UTR
GTAAAACAACAACTTATAAC+AGG 0.335247 3_3:-69563533 None:intergenic
ATTATGGTTCTCTATGTTGC+TGG 0.354926 3_3:+69562558 Msa0468050:CDS
AAATTGGGATTGACTGAGTT+TGG 0.366889 3_3:-69562133 None:intergenic
AATGGATAAAGTTGAAAATT+GGG 0.368376 3_3:-69562148 None:intergenic
ATGAGATAAAATAAAGTGTT+GGG 0.371291 3_3:-69562090 None:intergenic
TTCTTCTCATCATCTTGTCT+TGG 0.387442 3_3:-69562284 None:intergenic
AAGAGAAGGAAAATATGATT+TGG 0.392654 3_3:+69563650 Msa0468050:three_prime_UTR
GAAAATATGATTTGGCTCAT+TGG 0.398671 3_3:+69563658 Msa0468050:three_prime_UTR
CGGTTGCTCCGGTGGATGAT+TGG 0.406574 3_3:-69562397 None:intergenic
GTTACCACTCTCTCTGATAT+AGG 0.444657 3_3:+69562585 Msa0468050:CDS
GATAAATTTAACGGCGTTGG+TGG 0.454436 3_3:-69562375 None:intergenic
CGCCGTAACGGTTGCTCCGG+TGG 0.458989 3_3:-69562405 None:intergenic
AGCAGAGAGGCAAAACCCGC+AGG 0.469362 3_3:-69562450 None:intergenic
GGTTGAGAAGGATTGCAGAT+AGG 0.472984 3_3:-69562341 None:intergenic
GGATGATTGGATAAATTTAA+CGG 0.474216 3_3:-69562384 None:intergenic
AACTTATAACAGGGGAAGGA+GGG 0.477542 3_3:-69563523 None:intergenic
TCTTTACGCGGAGGTGGCTC+CGG 0.482440 3_3:-69562639 None:intergenic
AAGCTAATGTGAGTACCAAT+TGG 0.492910 3_3:+69563328 Msa0468050:exon
CCTCCGCGTAAAGATATTCC+AGG 0.497683 3_3:+69562648 Msa0468050:CDS
TCTGCTTCTGGGAATTTCGA+TGG 0.499850 3_3:+69562467 Msa0468050:CDS
CTGTTATATCACCGCGGCGC+CGG 0.499951 3_3:+69562620 Msa0468050:CDS
TTGGATAAATTTAACGGCGT+TGG 0.502757 3_3:-69562378 None:intergenic
TTGGGATTGACTGAGTTTGG+TGG 0.511014 3_3:-69562130 None:intergenic
GAGTTTGGTGGAAAGAAGAA+TGG 0.513029 3_3:-69562118 None:intergenic
CGGAGGTGGCTCCGGCGCCG+CGG 0.516650 3_3:-69562631 None:intergenic
GGCTCTGATGCTCTGTGGAT+CGG 0.521250 3_3:+69562488 Msa0468050:CDS
GGAGAAAGAAATCAAAGATA+CGG 0.526823 3_3:+69563349 Msa0468050:three_prime_UTR
TTAACGGCGTTGGTGGTGAA+CGG 0.531777 3_3:-69562368 None:intergenic
AATGAGATAAAATAAAGTGT+TGG 0.533363 3_3:-69562091 None:intergenic
GGAATATCTTTACGCGGAGG+TGG 0.535656 3_3:-69562645 None:intergenic
CAACTTATAACAGGGGAAGG+AGG 0.539505 3_3:-69563524 None:intergenic
GTGAACGGAGGAGGTTGAGA+AGG 0.548099 3_3:-69562353 None:intergenic
GCGTTGGTGGTGAACGGAGG+AGG 0.549273 3_3:-69562362 None:intergenic
CCACCTCAAATCTCACCTGC+GGG 0.549426 3_3:+69562435 Msa0468050:CDS
ACTTATAACAGGGGAAGGAG+GGG 0.552523 3_3:-69563522 None:intergenic
AAACGCCGTAACGGTTGCTC+CGG 0.554974 3_3:-69562408 None:intergenic
AAACCCGCAGGTGAGATTTG+AGG 0.566480 3_3:-69562438 None:intergenic
ATCCACCGGAGCAACCGTTA+CGG 0.568881 3_3:+69562403 Msa0468050:CDS
CTTATAACAGGGGAAGGAGG+GGG 0.570781 3_3:-69563521 None:intergenic
AAATTTATCCAATCATCCAC+CGG 0.571788 3_3:+69562389 Msa0468050:CDS
GAGAAGGAAGCTCCATAATG+TGG 0.580065 3_3:-69563418 None:intergenic
ATTGGGAGAAACGAGGCGAA+TGG 0.581574 3_3:-69562166 None:intergenic
CAACAACTTATAACAGGGGA+AGG 0.584055 3_3:-69563527 None:intergenic
TTGCATCTAATAAAAGGAGA+AGG 0.585689 3_3:-69563434 None:intergenic
GGTGGAGGTAAACGCCGTAA+CGG 0.594940 3_3:-69562417 None:intergenic
TATCATAAACTATAAAGAGA+AGG 0.595855 3_3:+69563636 Msa0468050:three_prime_UTR
TGAGATAAAATAAAGTGTTG+GGG 0.602981 3_3:-69562089 None:intergenic
GCAGGTGAGATTTGAGGTGG+AGG 0.603112 3_3:-69562432 None:intergenic
GATAAAATAAAGTGTTGGGG+GGG 0.607293 3_3:-69562086 None:intergenic
CCCGCAGGTGAGATTTGAGG+TGG 0.619368 3_3:-69562435 None:intergenic
TAAAACAACAACTTATAACA+GGG 0.620755 3_3:-69563532 None:intergenic
GAGATAAAATAAAGTGTTGG+GGG 0.625884 3_3:-69562088 None:intergenic
AGATAAAATAAAGTGTTGGG+GGG 0.628391 3_3:-69562087 None:intergenic
TCCACCTCAAATCTCACCTG+CGG 0.630396 3_3:+69562434 Msa0468050:CDS
ACGACCTATATCAGAGAGAG+TGG 0.640005 3_3:-69562589 None:intergenic
TCGATGGCTCTGATGCTCTG+TGG 0.642765 3_3:+69562483 Msa0468050:CDS
ACGGCGTTGGTGGTGAACGG+AGG 0.653070 3_3:-69562365 None:intergenic
AGGGTTTATTGGGAGAAACG+AGG 0.660421 3_3:-69562173 None:intergenic
ATAATCAATCCAATAACCCA+CGG 0.673735 3_3:-69562540 None:intergenic
CGAAATTCCCAGAAGCAGAG+AGG 0.678634 3_3:-69562463 None:intergenic
CCTGGAATATCTTTACGCGG+AGG 0.682081 3_3:-69562648 None:intergenic
TTACCTGGAATATCTTTACG+CGG 0.688091 3_3:-69562651 None:intergenic
AAAACAACAACTTATAACAG+GGG 0.691991 3_3:-69563531 None:intergenic
TGATAAGCAGAGCAGCGAGG+TGG 0.699742 3_3:-69562047 None:intergenic
TGTTCTCTGTTATATCACCG+CGG 0.748178 3_3:+69562614 Msa0468050:CDS
GAATGATAAGCAGAGCAGCG+AGG 0.754887 3_3:-69562050 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAGAAAAAAAAGATTAA+GGG - chr3_3:69562195-69562214 None:intergenic 15.0%
!! TCTATATACATAATGTATTA+AGG - chr3_3:69563101-69563120 None:intergenic 15.0%
!! AAGAAGAAAAAAAAGATTAA+GGG - chr3_3:69562195-69562214 None:intergenic 15.0%
!! TCTATATACATAATGTATTA+AGG - chr3_3:69563101-69563120 None:intergenic 15.0%
!!! ATGAGATAAAATAAAGTGTT+GGG - chr3_3:69562093-69562112 None:intergenic 20.0%
!! AATGAGATAAAATAAAGTGT+TGG - chr3_3:69562094-69562113 None:intergenic 20.0%
!!! AACACTTTATTTTATCTCAT+TGG + chr3_3:69562093-69562112 Msa0468050:five_prime_UTR 20.0%
!! AATGGATAAAGTTGAAAATT+GGG - chr3_3:69562151-69562170 None:intergenic 20.0%
!! AAAAAGATTAAGGGTTTATT+GGG - chr3_3:69562186-69562205 None:intergenic 20.0%
!! AAAAAAGATTAAGGGTTTAT+TGG - chr3_3:69562187-69562206 None:intergenic 20.0%
!! GAAGAAGAAAAAAAAGATTA+AGG - chr3_3:69562196-69562215 None:intergenic 20.0%
!! CAATGTTTATGTTTATGTTT+AGG - chr3_3:69562247-69562266 None:intergenic 20.0%
!!! TTAAGATTTTGTTTCGTATT+TGG + chr3_3:69562700-69562719 Msa0468050:intron 20.0%
!!! AAAGGTTTTTTTTTTGTGTT+GGG + chr3_3:69562773-69562792 Msa0468050:intron 20.0%
!!! TTTCAAATTTTCGAAAGAAA+TGG + chr3_3:69562825-69562844 Msa0468050:intron 20.0%
!!! TTCAAATTTTCGAAAGAAAT+GGG + chr3_3:69562826-69562845 Msa0468050:intron 20.0%
!! TAGTAATGAACTAAATGTTT+AGG + chr3_3:69563035-69563054 Msa0468050:intron 20.0%
!! AATGCATACATATATCTTAA+TGG - chr3_3:69563062-69563081 None:intergenic 20.0%
!!! TACATAATGTATTAAGGTTT+TGG - chr3_3:69563095-69563114 None:intergenic 20.0%
!! ACTACTCAAAATTTATTGTT+TGG + chr3_3:69563200-69563219 Msa0468050:intron 20.0%
!!! TAGGACAACATTTTTTTTAA+GGG - chr3_3:69563236-69563255 None:intergenic 20.0%
!! ATATAACAATTTCCACATTA+TGG + chr3_3:69563406-69563425 Msa0468050:three_prime_UTR 20.0%
!! TAAAACAACAACTTATAACA+GGG - chr3_3:69563535-69563554 None:intergenic 20.0%
!!! TATGTTTTAAACTGAACAAA+AGG - chr3_3:69563606-69563625 None:intergenic 20.0%
!! TATCATAAACTATAAAGAGA+AGG + chr3_3:69563636-69563655 Msa0468050:three_prime_UTR 20.0%
!!! ATGAGATAAAATAAAGTGTT+GGG - chr3_3:69562093-69562112 None:intergenic 20.0%
!! AATGAGATAAAATAAAGTGT+TGG - chr3_3:69562094-69562113 None:intergenic 20.0%
!!! AACACTTTATTTTATCTCAT+TGG + chr3_3:69562093-69562112 Msa0468050:five_prime_UTR 20.0%
!! AATGGATAAAGTTGAAAATT+GGG - chr3_3:69562151-69562170 None:intergenic 20.0%
!! AAAAAGATTAAGGGTTTATT+GGG - chr3_3:69562186-69562205 None:intergenic 20.0%
!! AAAAAAGATTAAGGGTTTAT+TGG - chr3_3:69562187-69562206 None:intergenic 20.0%
!! GAAGAAGAAAAAAAAGATTA+AGG - chr3_3:69562196-69562215 None:intergenic 20.0%
!! CAATGTTTATGTTTATGTTT+AGG - chr3_3:69562247-69562266 None:intergenic 20.0%
!!! TTAAGATTTTGTTTCGTATT+TGG + chr3_3:69562700-69562719 Msa0468050:intron 20.0%
!!! AAAGGTTTTTTTTTTGTGTT+GGG + chr3_3:69562773-69562792 Msa0468050:intron 20.0%
!!! TTTCAAATTTTCGAAAGAAA+TGG + chr3_3:69562825-69562844 Msa0468050:intron 20.0%
!!! TTCAAATTTTCGAAAGAAAT+GGG + chr3_3:69562826-69562845 Msa0468050:intron 20.0%
!! TAGTAATGAACTAAATGTTT+AGG + chr3_3:69563035-69563054 Msa0468050:intron 20.0%
!! AATGCATACATATATCTTAA+TGG - chr3_3:69563062-69563081 None:intergenic 20.0%
!!! TACATAATGTATTAAGGTTT+TGG - chr3_3:69563095-69563114 None:intergenic 20.0%
!! ACTACTCAAAATTTATTGTT+TGG + chr3_3:69563200-69563219 Msa0468050:intron 20.0%
!!! TAGGACAACATTTTTTTTAA+GGG - chr3_3:69563236-69563255 None:intergenic 20.0%
!! ATATAACAATTTCCACATTA+TGG + chr3_3:69563406-69563425 Msa0468050:three_prime_UTR 20.0%
!! TAAAACAACAACTTATAACA+GGG - chr3_3:69563535-69563554 None:intergenic 20.0%
!!! TATGTTTTAAACTGAACAAA+AGG - chr3_3:69563606-69563625 None:intergenic 20.0%
!! TATCATAAACTATAAAGAGA+AGG + chr3_3:69563636-69563655 Msa0468050:three_prime_UTR 20.0%
!!! TGAGATAAAATAAAGTGTTG+GGG - chr3_3:69562092-69562111 None:intergenic 25.0%
! GAATGGATAAAGTTGAAAAT+TGG - chr3_3:69562152-69562171 None:intergenic 25.0%
! GGATGATTGGATAAATTTAA+CGG - chr3_3:69562387-69562406 None:intergenic 25.0%
!! GATAACAACAAAACTTTTGT+CGG + chr3_3:69562674-69562693 Msa0468050:intron 25.0%
! GTTGTTCAATGATTTAACAA+AGG + chr3_3:69562755-69562774 Msa0468050:intron 25.0%
!!! CAAAGGTTTTTTTTTTGTGT+TGG + chr3_3:69562772-69562791 Msa0468050:intron 25.0%
!!! TTTTTTTTTGTGTTGGGTTT+TGG + chr3_3:69562779-69562798 Msa0468050:intron 25.0%
!!! TTTTTTTTGTGTTGGGTTTT+GGG + chr3_3:69562780-69562799 Msa0468050:intron 25.0%
!!! CTAGGACAACATTTTTTTTA+AGG - chr3_3:69563237-69563256 None:intergenic 25.0%
! AATCTCTTTGCATTACAAAT+AGG + chr3_3:69563481-69563500 Msa0468050:three_prime_UTR 25.0%
! ATCTCTTTGCATTACAAATA+GGG + chr3_3:69563482-69563501 Msa0468050:three_prime_UTR 25.0%
! AAAACAACAACTTATAACAG+GGG - chr3_3:69563534-69563553 None:intergenic 25.0%
! GTAAAACAACAACTTATAAC+AGG - chr3_3:69563536-69563555 None:intergenic 25.0%
! AAGAGAAGGAAAATATGATT+TGG + chr3_3:69563650-69563669 Msa0468050:three_prime_UTR 25.0%
!!! TGAGATAAAATAAAGTGTTG+GGG - chr3_3:69562092-69562111 None:intergenic 25.0%
! GAATGGATAAAGTTGAAAAT+TGG - chr3_3:69562152-69562171 None:intergenic 25.0%
! GGATGATTGGATAAATTTAA+CGG - chr3_3:69562387-69562406 None:intergenic 25.0%
!! GATAACAACAAAACTTTTGT+CGG + chr3_3:69562674-69562693 Msa0468050:intron 25.0%
! GTTGTTCAATGATTTAACAA+AGG + chr3_3:69562755-69562774 Msa0468050:intron 25.0%
!!! CAAAGGTTTTTTTTTTGTGT+TGG + chr3_3:69562772-69562791 Msa0468050:intron 25.0%
!!! TTTTTTTTTGTGTTGGGTTT+TGG + chr3_3:69562779-69562798 Msa0468050:intron 25.0%
!!! TTTTTTTTGTGTTGGGTTTT+GGG + chr3_3:69562780-69562799 Msa0468050:intron 25.0%
!!! CTAGGACAACATTTTTTTTA+AGG - chr3_3:69563237-69563256 None:intergenic 25.0%
! AATCTCTTTGCATTACAAAT+AGG + chr3_3:69563481-69563500 Msa0468050:three_prime_UTR 25.0%
! ATCTCTTTGCATTACAAATA+GGG + chr3_3:69563482-69563501 Msa0468050:three_prime_UTR 25.0%
! AAAACAACAACTTATAACAG+GGG - chr3_3:69563534-69563553 None:intergenic 25.0%
! GTAAAACAACAACTTATAAC+AGG - chr3_3:69563536-69563555 None:intergenic 25.0%
! AAGAGAAGGAAAATATGATT+TGG + chr3_3:69563650-69563669 Msa0468050:three_prime_UTR 25.0%
!! AGATAAAATAAAGTGTTGGG+GGG - chr3_3:69562090-69562109 None:intergenic 30.0%
!! GAGATAAAATAAAGTGTTGG+GGG - chr3_3:69562091-69562110 None:intergenic 30.0%
AAATTTATCCAATCATCCAC+CGG + chr3_3:69562389-69562408 Msa0468050:CDS 30.0%
ATAATCAATCCAATAACCCA+CGG - chr3_3:69562543-69562562 None:intergenic 30.0%
!!! AGTTTTGTTGTTATCTTACC+TGG - chr3_3:69562669-69562688 None:intergenic 30.0%
AAACAAGTACGAGACAAAAA+AGG - chr3_3:69562735-69562754 None:intergenic 30.0%
! GGATGCAAATTCATATGTTT+TGG + chr3_3:69562847-69562866 Msa0468050:intron 30.0%
AGCGAAAATCGAACATATTT+GGG - chr3_3:69562880-69562899 None:intergenic 30.0%
GTGCAATAAGCAAAATTTGT+TGG + chr3_3:69562962-69562981 Msa0468050:intron 30.0%
TGGTTTGATCATAAAAGACA+AGG + chr3_3:69562982-69563001 Msa0468050:intron 30.0%
TTAAGCCACACATGATAAAA+GGG - chr3_3:69563280-69563299 None:intergenic 30.0%
ATTAAGCCACACATGATAAA+AGG - chr3_3:69563281-69563300 None:intergenic 30.0%
! CTTTGATTTCTTTCTCCAAT+TGG - chr3_3:69563346-69563365 None:intergenic 30.0%
GGAGAAAGAAATCAAAGATA+CGG + chr3_3:69563349-69563368 Msa0468050:three_prime_UTR 30.0%
TTGCATCTAATAAAAGGAGA+AGG - chr3_3:69563437-69563456 None:intergenic 30.0%
! TTCTCCTTTTATTAGATGCA+AGG + chr3_3:69563436-69563455 Msa0468050:three_prime_UTR 30.0%
GTAACCTTGCATCTAATAAA+AGG - chr3_3:69563443-69563462 None:intergenic 30.0%
! TGCAAGGTTACTAAATTTTG+TGG + chr3_3:69563452-69563471 Msa0468050:three_prime_UTR 30.0%
! GAAAATATGATTTGGCTCAT+TGG + chr3_3:69563658-69563677 Msa0468050:three_prime_UTR 30.0%
!! AGATAAAATAAAGTGTTGGG+GGG - chr3_3:69562090-69562109 None:intergenic 30.0%
!! GAGATAAAATAAAGTGTTGG+GGG - chr3_3:69562091-69562110 None:intergenic 30.0%
AAATTTATCCAATCATCCAC+CGG + chr3_3:69562389-69562408 Msa0468050:CDS 30.0%
ATAATCAATCCAATAACCCA+CGG - chr3_3:69562543-69562562 None:intergenic 30.0%
!!! AGTTTTGTTGTTATCTTACC+TGG - chr3_3:69562669-69562688 None:intergenic 30.0%
AAACAAGTACGAGACAAAAA+AGG - chr3_3:69562735-69562754 None:intergenic 30.0%
! GGATGCAAATTCATATGTTT+TGG + chr3_3:69562847-69562866 Msa0468050:intron 30.0%
AGCGAAAATCGAACATATTT+GGG - chr3_3:69562880-69562899 None:intergenic 30.0%
GTGCAATAAGCAAAATTTGT+TGG + chr3_3:69562962-69562981 Msa0468050:intron 30.0%
TGGTTTGATCATAAAAGACA+AGG + chr3_3:69562982-69563001 Msa0468050:intron 30.0%
TTAAGCCACACATGATAAAA+GGG - chr3_3:69563280-69563299 None:intergenic 30.0%
ATTAAGCCACACATGATAAA+AGG - chr3_3:69563281-69563300 None:intergenic 30.0%
! CTTTGATTTCTTTCTCCAAT+TGG - chr3_3:69563346-69563365 None:intergenic 30.0%
GGAGAAAGAAATCAAAGATA+CGG + chr3_3:69563349-69563368 Msa0468050:three_prime_UTR 30.0%
TTGCATCTAATAAAAGGAGA+AGG - chr3_3:69563437-69563456 None:intergenic 30.0%
! TTCTCCTTTTATTAGATGCA+AGG + chr3_3:69563436-69563455 Msa0468050:three_prime_UTR 30.0%
GTAACCTTGCATCTAATAAA+AGG - chr3_3:69563443-69563462 None:intergenic 30.0%
! TGCAAGGTTACTAAATTTTG+TGG + chr3_3:69563452-69563471 Msa0468050:three_prime_UTR 30.0%
! GAAAATATGATTTGGCTCAT+TGG + chr3_3:69563658-69563677 Msa0468050:three_prime_UTR 30.0%
!! GATAAAATAAAGTGTTGGGG+GGG - chr3_3:69562089-69562108 None:intergenic 35.0%
AAATTGGGATTGACTGAGTT+TGG - chr3_3:69562136-69562155 None:intergenic 35.0%
TTCTTCTCATCATCTTGTCT+TGG - chr3_3:69562287-69562306 None:intergenic 35.0%
TTGGATAAATTTAACGGCGT+TGG - chr3_3:69562381-69562400 None:intergenic 35.0%
! GTGGGTTATTGGATTGATTA+TGG + chr3_3:69562542-69562561 Msa0468050:CDS 35.0%
ATTATGGTTCTCTATGTTGC+TGG + chr3_3:69562558-69562577 Msa0468050:CDS 35.0%
TTACCTGGAATATCTTTACG+CGG - chr3_3:69562654-69562673 None:intergenic 35.0%
GCGAAAATCGAACATATTTG+GGG - chr3_3:69562879-69562898 None:intergenic 35.0%
CAGCGAAAATCGAACATATT+TGG - chr3_3:69562881-69562900 None:intergenic 35.0%
! TATGTTCGATTTTCGCTGAT+TGG + chr3_3:69562883-69562902 Msa0468050:intron 35.0%
!! ATGTTCGATTTTCGCTGATT+GGG + chr3_3:69562884-69562903 Msa0468050:intron 35.0%
!! GGTCTTGTTTCTTCATCAAA+TGG + chr3_3:69562905-69562924 Msa0468050:intron 35.0%
TCTTCATCAAATGGAACCTA+CGG + chr3_3:69562914-69562933 Msa0468050:intron 35.0%
CTAACTTTGAGCTGAATTAG+AGG + chr3_3:69562940-69562959 Msa0468050:intron 35.0%
AAGGTGTATGTGAATTTCGT+GGG + chr3_3:69563001-69563020 Msa0468050:intron 35.0%
!!! AGCTGCTTTGCTTTTTAAGA+AGG - chr3_3:69563177-69563196 None:intergenic 35.0%
AAAAAATGTTGTCCTAGCTG+CGG + chr3_3:69563240-69563259 Msa0468050:intron 35.0%
TAAGCCACACATGATAAAAG+GGG - chr3_3:69563279-69563298 None:intergenic 35.0%
AAGCTAATGTGAGTACCAAT+TGG + chr3_3:69563328-69563347 Msa0468050:exon 35.0%
!! GATAAAATAAAGTGTTGGGG+GGG - chr3_3:69562089-69562108 None:intergenic 35.0%
AAATTGGGATTGACTGAGTT+TGG - chr3_3:69562136-69562155 None:intergenic 35.0%
TTCTTCTCATCATCTTGTCT+TGG - chr3_3:69562287-69562306 None:intergenic 35.0%
TTGGATAAATTTAACGGCGT+TGG - chr3_3:69562381-69562400 None:intergenic 35.0%
! GTGGGTTATTGGATTGATTA+TGG + chr3_3:69562542-69562561 Msa0468050:CDS 35.0%
ATTATGGTTCTCTATGTTGC+TGG + chr3_3:69562558-69562577 Msa0468050:CDS 35.0%
TTACCTGGAATATCTTTACG+CGG - chr3_3:69562654-69562673 None:intergenic 35.0%
GCGAAAATCGAACATATTTG+GGG - chr3_3:69562879-69562898 None:intergenic 35.0%
CAGCGAAAATCGAACATATT+TGG - chr3_3:69562881-69562900 None:intergenic 35.0%
! TATGTTCGATTTTCGCTGAT+TGG + chr3_3:69562883-69562902 Msa0468050:intron 35.0%
!! ATGTTCGATTTTCGCTGATT+GGG + chr3_3:69562884-69562903 Msa0468050:intron 35.0%
!! GGTCTTGTTTCTTCATCAAA+TGG + chr3_3:69562905-69562924 Msa0468050:intron 35.0%
TCTTCATCAAATGGAACCTA+CGG + chr3_3:69562914-69562933 Msa0468050:intron 35.0%
CTAACTTTGAGCTGAATTAG+AGG + chr3_3:69562940-69562959 Msa0468050:intron 35.0%
AAGGTGTATGTGAATTTCGT+GGG + chr3_3:69563001-69563020 Msa0468050:intron 35.0%
!!! AGCTGCTTTGCTTTTTAAGA+AGG - chr3_3:69563177-69563196 None:intergenic 35.0%
AAAAAATGTTGTCCTAGCTG+CGG + chr3_3:69563240-69563259 Msa0468050:intron 35.0%
TAAGCCACACATGATAAAAG+GGG - chr3_3:69563279-69563298 None:intergenic 35.0%
AAGCTAATGTGAGTACCAAT+TGG + chr3_3:69563328-69563347 Msa0468050:exon 35.0%
! GAGTTTGGTGGAAAGAAGAA+TGG - chr3_3:69562121-69562140 None:intergenic 40.0%
! GATAAATTTAACGGCGTTGG+TGG - chr3_3:69562378-69562397 None:intergenic 40.0%
!!! TTGGATTTGTTTTGATGCCG+TGG + chr3_3:69562523-69562542 Msa0468050:CDS 40.0%
!!! TGGATTTGTTTTGATGCCGT+GGG + chr3_3:69562524-69562543 Msa0468050:CDS 40.0%
GTTACCACTCTCTCTGATAT+AGG + chr3_3:69562585-69562604 Msa0468050:CDS 40.0%
TGTTCTCTGTTATATCACCG+CGG + chr3_3:69562614-69562633 Msa0468050:CDS 40.0%
AGCTCAAAGTTAGAAACCGT+AGG - chr3_3:69562933-69562952 None:intergenic 40.0%
CAAGGTGTATGTGAATTTCG+TGG + chr3_3:69563000-69563019 Msa0468050:intron 40.0%
! GCTTTTTGTTGCGTTGCATT+AGG + chr3_3:69563138-69563157 Msa0468050:intron 40.0%
! AAATTTGTGTTGTTGCAGCG+TGG + chr3_3:69563305-69563324 Msa0468050:intron 40.0%
!! AGATACGGCGTTTGATTTTG+TGG + chr3_3:69563364-69563383 Msa0468050:three_prime_UTR 40.0%
AACTTATAACAGGGGAAGGA+GGG - chr3_3:69563526-69563545 None:intergenic 40.0%
CAACAACTTATAACAGGGGA+AGG - chr3_3:69563530-69563549 None:intergenic 40.0%
! GAGTTTGGTGGAAAGAAGAA+TGG - chr3_3:69562121-69562140 None:intergenic 40.0%
! GATAAATTTAACGGCGTTGG+TGG - chr3_3:69562378-69562397 None:intergenic 40.0%
!!! TTGGATTTGTTTTGATGCCG+TGG + chr3_3:69562523-69562542 Msa0468050:CDS 40.0%
!!! TGGATTTGTTTTGATGCCGT+GGG + chr3_3:69562524-69562543 Msa0468050:CDS 40.0%
GTTACCACTCTCTCTGATAT+AGG + chr3_3:69562585-69562604 Msa0468050:CDS 40.0%
TGTTCTCTGTTATATCACCG+CGG + chr3_3:69562614-69562633 Msa0468050:CDS 40.0%
AGCTCAAAGTTAGAAACCGT+AGG - chr3_3:69562933-69562952 None:intergenic 40.0%
CAAGGTGTATGTGAATTTCG+TGG + chr3_3:69563000-69563019 Msa0468050:intron 40.0%
! GCTTTTTGTTGCGTTGCATT+AGG + chr3_3:69563138-69563157 Msa0468050:intron 40.0%
! AAATTTGTGTTGTTGCAGCG+TGG + chr3_3:69563305-69563324 Msa0468050:intron 40.0%
!! AGATACGGCGTTTGATTTTG+TGG + chr3_3:69563364-69563383 Msa0468050:three_prime_UTR 40.0%
AACTTATAACAGGGGAAGGA+GGG - chr3_3:69563526-69563545 None:intergenic 40.0%
CAACAACTTATAACAGGGGA+AGG - chr3_3:69563530-69563549 None:intergenic 40.0%
TTGGGATTGACTGAGTTTGG+TGG - chr3_3:69562133-69562152 None:intergenic 45.0%
AGGGTTTATTGGGAGAAACG+AGG - chr3_3:69562176-69562195 None:intergenic 45.0%
GGTTGAGAAGGATTGCAGAT+AGG - chr3_3:69562344-69562363 None:intergenic 45.0%
! TCTGCTTCTGGGAATTTCGA+TGG + chr3_3:69562467-69562486 Msa0468050:CDS 45.0%
GGATCGGTCACGTTCTTTAT+TGG + chr3_3:69562504-69562523 Msa0468050:CDS 45.0%
!! GTTTTGATGCCGTGGGTTAT+TGG + chr3_3:69562531-69562550 Msa0468050:CDS 45.0%
ACGACCTATATCAGAGAGAG+TGG - chr3_3:69562592-69562611 None:intergenic 45.0%
! TTTTGGGCGTGTGAGAACAT+AGG + chr3_3:69562796-69562815 Msa0468050:intron 45.0%
TTTGGGCGTGTGAGAACATA+GGG + chr3_3:69562797-69562816 Msa0468050:intron 45.0%
CCACACATGATAAAAGGGGT+GGG - chr3_3:69563275-69563294 None:intergenic 45.0%
GAGAAGGAAGCTCCATAATG+TGG - chr3_3:69563421-69563440 None:intergenic 45.0%
ACTTATAACAGGGGAAGGAG+GGG - chr3_3:69563525-69563544 None:intergenic 45.0%
CAACTTATAACAGGGGAAGG+AGG - chr3_3:69563527-69563546 None:intergenic 45.0%
TTGGGATTGACTGAGTTTGG+TGG - chr3_3:69562133-69562152 None:intergenic 45.0%
AGGGTTTATTGGGAGAAACG+AGG - chr3_3:69562176-69562195 None:intergenic 45.0%
GGTTGAGAAGGATTGCAGAT+AGG - chr3_3:69562344-69562363 None:intergenic 45.0%
! TCTGCTTCTGGGAATTTCGA+TGG + chr3_3:69562467-69562486 Msa0468050:CDS 45.0%
GGATCGGTCACGTTCTTTAT+TGG + chr3_3:69562504-69562523 Msa0468050:CDS 45.0%
!! GTTTTGATGCCGTGGGTTAT+TGG + chr3_3:69562531-69562550 Msa0468050:CDS 45.0%
ACGACCTATATCAGAGAGAG+TGG - chr3_3:69562592-69562611 None:intergenic 45.0%
! TTTTGGGCGTGTGAGAACAT+AGG + chr3_3:69562796-69562815 Msa0468050:intron 45.0%
TTTGGGCGTGTGAGAACATA+GGG + chr3_3:69562797-69562816 Msa0468050:intron 45.0%
CCACACATGATAAAAGGGGT+GGG - chr3_3:69563275-69563294 None:intergenic 45.0%
GAGAAGGAAGCTCCATAATG+TGG - chr3_3:69563421-69563440 None:intergenic 45.0%
ACTTATAACAGGGGAAGGAG+GGG - chr3_3:69563525-69563544 None:intergenic 45.0%
CAACTTATAACAGGGGAAGG+AGG - chr3_3:69563527-69563546 None:intergenic 45.0%
GAATGATAAGCAGAGCAGCG+AGG - chr3_3:69562053-69562072 None:intergenic 50.0%
ATTGGGAGAAACGAGGCGAA+TGG - chr3_3:69562169-69562188 None:intergenic 50.0%
!! TTAACGGCGTTGGTGGTGAA+CGG - chr3_3:69562371-69562390 None:intergenic 50.0%
TCCACCTCAAATCTCACCTG+CGG + chr3_3:69562434-69562453 Msa0468050:CDS 50.0%
AAACCCGCAGGTGAGATTTG+AGG - chr3_3:69562441-69562460 None:intergenic 50.0%
! GGTTTTGCCTCTCTGCTTCT+GGG + chr3_3:69562456-69562475 Msa0468050:CDS 50.0%
CGAAATTCCCAGAAGCAGAG+AGG - chr3_3:69562466-69562485 None:intergenic 50.0%
GGAATATCTTTACGCGGAGG+TGG - chr3_3:69562648-69562667 None:intergenic 50.0%
CCTGGAATATCTTTACGCGG+AGG - chr3_3:69562651-69562670 None:intergenic 50.0%
CCTCCGCGTAAAGATATTCC+AGG + chr3_3:69562648-69562667 Msa0468050:CDS 50.0%
GCGTGTGAGAACATAGGGAA+AGG + chr3_3:69562802-69562821 Msa0468050:intron 50.0%
GATAAAAGGGGTGGGCAAGA+GGG - chr3_3:69563267-69563286 None:intergenic 50.0%
TGATAAAAGGGGTGGGCAAG+AGG - chr3_3:69563268-69563287 None:intergenic 50.0%
! CCCACCCCTTTTATCATGTG+TGG + chr3_3:69563272-69563291 Msa0468050:intron 50.0%
GCCACACATGATAAAAGGGG+TGG - chr3_3:69563276-69563295 None:intergenic 50.0%
CTTATAACAGGGGAAGGAGG+GGG - chr3_3:69563524-69563543 None:intergenic 50.0%
GAATGATAAGCAGAGCAGCG+AGG - chr3_3:69562053-69562072 None:intergenic 50.0%
ATTGGGAGAAACGAGGCGAA+TGG - chr3_3:69562169-69562188 None:intergenic 50.0%
!! TTAACGGCGTTGGTGGTGAA+CGG - chr3_3:69562371-69562390 None:intergenic 50.0%
TCCACCTCAAATCTCACCTG+CGG + chr3_3:69562434-69562453 Msa0468050:CDS 50.0%
AAACCCGCAGGTGAGATTTG+AGG - chr3_3:69562441-69562460 None:intergenic 50.0%
! GGTTTTGCCTCTCTGCTTCT+GGG + chr3_3:69562456-69562475 Msa0468050:CDS 50.0%
CGAAATTCCCAGAAGCAGAG+AGG - chr3_3:69562466-69562485 None:intergenic 50.0%
GGAATATCTTTACGCGGAGG+TGG - chr3_3:69562648-69562667 None:intergenic 50.0%
CCTGGAATATCTTTACGCGG+AGG - chr3_3:69562651-69562670 None:intergenic 50.0%
CCTCCGCGTAAAGATATTCC+AGG + chr3_3:69562648-69562667 Msa0468050:CDS 50.0%
GCGTGTGAGAACATAGGGAA+AGG + chr3_3:69562802-69562821 Msa0468050:intron 50.0%
GATAAAAGGGGTGGGCAAGA+GGG - chr3_3:69563267-69563286 None:intergenic 50.0%
TGATAAAAGGGGTGGGCAAG+AGG - chr3_3:69563268-69563287 None:intergenic 50.0%
! CCCACCCCTTTTATCATGTG+TGG + chr3_3:69563272-69563291 Msa0468050:intron 50.0%
GCCACACATGATAAAAGGGG+TGG - chr3_3:69563276-69563295 None:intergenic 50.0%
CTTATAACAGGGGAAGGAGG+GGG - chr3_3:69563524-69563543 None:intergenic 50.0%
GTGAACGGAGGAGGTTGAGA+AGG - chr3_3:69562356-69562375 None:intergenic 55.0%
ATCCACCGGAGCAACCGTTA+CGG + chr3_3:69562403-69562422 Msa0468050:CDS 55.0%
AAACGCCGTAACGGTTGCTC+CGG - chr3_3:69562411-69562430 None:intergenic 55.0%
GGTGGAGGTAAACGCCGTAA+CGG - chr3_3:69562420-69562439 None:intergenic 55.0%
! GCAGGTGAGATTTGAGGTGG+AGG - chr3_3:69562435-69562454 None:intergenic 55.0%
CCACCTCAAATCTCACCTGC+GGG + chr3_3:69562435-69562454 Msa0468050:CDS 55.0%
! GGGTTTTGCCTCTCTGCTTC+TGG + chr3_3:69562455-69562474 Msa0468050:CDS 55.0%
!! TCGATGGCTCTGATGCTCTG+TGG + chr3_3:69562483-69562502 Msa0468050:CDS 55.0%
! GGCTCTGATGCTCTGTGGAT+CGG + chr3_3:69562488-69562507 Msa0468050:CDS 55.0%
! GTGAATTTCGTGGGAGCGTG+AGG + chr3_3:69563010-69563029 Msa0468050:intron 55.0%
GTGAACGGAGGAGGTTGAGA+AGG - chr3_3:69562356-69562375 None:intergenic 55.0%
ATCCACCGGAGCAACCGTTA+CGG + chr3_3:69562403-69562422 Msa0468050:CDS 55.0%
AAACGCCGTAACGGTTGCTC+CGG - chr3_3:69562411-69562430 None:intergenic 55.0%
GGTGGAGGTAAACGCCGTAA+CGG - chr3_3:69562420-69562439 None:intergenic 55.0%
! GCAGGTGAGATTTGAGGTGG+AGG - chr3_3:69562435-69562454 None:intergenic 55.0%
CCACCTCAAATCTCACCTGC+GGG + chr3_3:69562435-69562454 Msa0468050:CDS 55.0%
! GGGTTTTGCCTCTCTGCTTC+TGG + chr3_3:69562455-69562474 Msa0468050:CDS 55.0%
!! TCGATGGCTCTGATGCTCTG+TGG + chr3_3:69562483-69562502 Msa0468050:CDS 55.0%
! GGCTCTGATGCTCTGTGGAT+CGG + chr3_3:69562488-69562507 Msa0468050:CDS 55.0%
! GTGAATTTCGTGGGAGCGTG+AGG + chr3_3:69563010-69563029 Msa0468050:intron 55.0%
CGGTTGCTCCGGTGGATGAT+TGG - chr3_3:69562400-69562419 None:intergenic 60.0%
CCCGCAGGTGAGATTTGAGG+TGG - chr3_3:69562438-69562457 None:intergenic 60.0%
AGCAGAGAGGCAAAACCCGC+AGG - chr3_3:69562453-69562472 None:intergenic 60.0%
CTGTTATATCACCGCGGCGC+CGG + chr3_3:69562620-69562639 Msa0468050:CDS 60.0%
TCTTTACGCGGAGGTGGCTC+CGG - chr3_3:69562642-69562661 None:intergenic 60.0%
CGGTTGCTCCGGTGGATGAT+TGG - chr3_3:69562400-69562419 None:intergenic 60.0%
CCCGCAGGTGAGATTTGAGG+TGG - chr3_3:69562438-69562457 None:intergenic 60.0%
AGCAGAGAGGCAAAACCCGC+AGG - chr3_3:69562453-69562472 None:intergenic 60.0%
CTGTTATATCACCGCGGCGC+CGG + chr3_3:69562620-69562639 Msa0468050:CDS 60.0%
TCTTTACGCGGAGGTGGCTC+CGG - chr3_3:69562642-69562661 None:intergenic 60.0%
!! GCGTTGGTGGTGAACGGAGG+AGG - chr3_3:69562365-69562384 None:intergenic 65.0%
!! ACGGCGTTGGTGGTGAACGG+AGG - chr3_3:69562368-69562387 None:intergenic 65.0%
!! GCGTTGGTGGTGAACGGAGG+AGG - chr3_3:69562365-69562384 None:intergenic 65.0%
!! ACGGCGTTGGTGGTGAACGG+AGG - chr3_3:69562368-69562387 None:intergenic 65.0%
CGCCGTAACGGTTGCTCCGG+TGG - chr3_3:69562408-69562427 None:intergenic 70.0%
GGGCAAGAGGGACCGCAGCT+AGG - chr3_3:69563255-69563274 None:intergenic 70.0%
CGCCGTAACGGTTGCTCCGG+TGG - chr3_3:69562408-69562427 None:intergenic 70.0%
GGGCAAGAGGGACCGCAGCT+AGG - chr3_3:69563255-69563274 None:intergenic 70.0%
! CGGAGGTGGCTCCGGCGCCG+CGG - chr3_3:69562634-69562653 None:intergenic 85.0%
! CGGAGGTGGCTCCGGCGCCG+CGG - chr3_3:69562634-69562653 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 69562050 69563685 69562050 ID=Msa0468050;Name=Msa0468050
chr3_3 mRNA 69562050 69563685 69562050 ID=Msa0468050-mRNA-1;Parent=Msa0468050;Name=Msa0468050-mRNA-1;_AED=0.00;_eAED=0.00;_QI=244|1|1|1|1|1|2|346|130
chr3_3 exon 69562050 69562669 69562050 ID=Msa0468050-mRNA-1:exon:16454;Parent=Msa0468050-mRNA-1
chr3_3 exon 69563323 69563685 69563323 ID=Msa0468050-mRNA-1:exon:16455;Parent=Msa0468050-mRNA-1
chr3_3 five_prime_UTR 69562050 69562293 69562050 ID=Msa0468050-mRNA-1:five_prime_utr;Parent=Msa0468050-mRNA-1
chr3_3 CDS 69562294 69562669 69562294 ID=Msa0468050-mRNA-1:cds;Parent=Msa0468050-mRNA-1
chr3_3 CDS 69563323 69563339 69563323 ID=Msa0468050-mRNA-1:cds;Parent=Msa0468050-mRNA-1
chr3_3 three_prime_UTR 69563340 69563685 69563340 ID=Msa0468050-mRNA-1:three_prime_utr;Parent=Msa0468050-mRNA-1
Gene Sequence

>Msa0468050

ATGATGAGAAGAACACGTGAAGATGAACAATCACGAGCTTTCTACGACCTATCTGCAATCCTTCTCAACCTCCTCCGTTCACCACCAACGCCGTTAAATTTATCCAATCATCCACCGGAGCAACCGTTACGGCGTTTACCTCCACCTCAAATCTCACCTGCGGGTTTTGCCTCTCTGCTTCTGGGAATTTCGATGGCTCTGATGCTCTGTGGATCGGTCACGTTCTTTATTGGATTTGTTTTGATGCCGTGGGTTATTGGATTGATTATGGTTCTCTATGTTGCTGGAATTGTTACCACTCTCTCTGATATAGGTCGTTCTGTTCTCTGTTATATCACCGCGGCGCCGGAGCCACCTCCGCGTAAAGATATTCCAGCGTGGAAGCTAATGTGA
Protein sequence

>Msa0468050

MMRRTREDEQSRAFYDLSAILLNLLRSPPTPLNLSNHPPEQPLRRLPPPQISPAGFASLLLGISMALMLCGSVTFFIGFVLMPWVIGLIMVLYVAGIVTTLSDIGRSVLCYITAAPEPPPRKDIPAWKLM*