AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0604940

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0604940 MtrunA17_Chr4g0033751 85.780 218 6 1 1 193 191 408 1.03e-122 363
Msa0604940 MtrunA17_Chr2g0329581 53.960 202 45 6 20 193 241 422 5.69e-51 174
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0604940 AT1G08760.1 43.662 213 84 9 11 193 223 429 1.75e-42 151

Find 52 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAACCTTGACAAAGTTTC+CGG 0.253676 4_2:+50721484 None:intergenic
TCTTCAGAAGCCGGACTTCC+CGG 0.331048 4_2:-50721503 Msa0604940:CDS
TTACTCTCGTAATCTTTAGA+AGG 0.353943 4_2:+50721656 None:intergenic
GTCATAACAGACAGGATCTT+TGG 0.354014 4_2:+50721178 None:intergenic
TCAATCAGAAATTTAGTTCA+AGG 0.364637 4_2:-50722291 Msa0604940:CDS
ACGAAGCAAAGGAACTCTTT+TGG 0.370193 4_2:-50721533 Msa0604940:CDS
AACACCAACCAAGTCCTCTA+AGG 0.411554 4_2:-50721627 Msa0604940:CDS
GACAAAGTTTCCGGGAAGTC+CGG 0.419326 4_2:+50721493 None:intergenic
AGTTAACGGTGATGGAACTA+TGG 0.422657 4_2:-50721573 Msa0604940:CDS
GAGAATTTCTCAAAAGAAGT+AGG 0.433004 4_2:+50722441 None:intergenic
CCTTCATCTATTGCGAAGCT+TGG 0.438351 4_2:-50721413 Msa0604940:CDS
CTAAGGCGTTGCGGAAAAGC+TGG 0.438960 4_2:-50722254 Msa0604940:CDS
GAAACCTTGACAAAGTTTCC+GGG 0.443615 4_2:+50721485 None:intergenic
TAGTTGTATCATTAGTTAGT+TGG 0.445623 4_2:-50721334 Msa0604940:three_prime_UTR
GGGGTGGTGGAGACGGGAAA+GGG 0.448470 4_2:-50722360 Msa0604940:CDS
AAATCTATAAAGAAAGTTAA+CGG 0.457905 4_2:-50721587 Msa0604940:CDS
CTTCATCTATTGCGAAGCTT+GGG 0.468822 4_2:-50721412 Msa0604940:CDS
GGAAAGAAATCTTCAGAAGC+CGG 0.471468 4_2:-50721512 Msa0604940:CDS
CATCTATTGCGAAGCTTGGG+AGG 0.483470 4_2:-50721409 Msa0604940:CDS
TAGCAACTAGTAGGTGAAGA+AGG 0.490380 4_2:+50722471 None:intergenic
CCAAGCTTCGCAATAGATGA+AGG 0.500292 4_2:+50721413 None:intergenic
GTTCGCGGAGTTAGTCCTCT+CGG 0.502784 4_2:-50722336 Msa0604940:CDS
TTCTGGTGTTCCAGACTCCT+AGG 0.504156 4_2:-50721703 Msa0604940:intron
TGACAGAAGCAAGTGTTCAA+TGG 0.504746 4_2:-50721445 Msa0604940:CDS
GGATAGGCTGATTGCTAAGG+TGG 0.505127 4_2:-50722382 Msa0604940:CDS
GGCAAAGAATAGCAACTAGT+AGG 0.507800 4_2:+50722462 None:intergenic
TTAGTTCAAGGAATTGAACT+TGG 0.515121 4_2:-50722279 Msa0604940:CDS
AAGCAACAAGGAAATATCAA+TGG 0.517246 4_2:-50722408 Msa0604940:CDS
TCTTCCTTAGAGGACTTGGT+TGG 0.518185 4_2:+50721623 None:intergenic
ATAAAGAAAGTTAACGGTGA+TGG 0.518959 4_2:-50721581 Msa0604940:CDS
GAGACGGGAAAGGGTGTTCG+CGG 0.527317 4_2:-50722351 Msa0604940:CDS
AGGAATTGAACTTGGTGCTA+AGG 0.528575 4_2:-50722271 Msa0604940:CDS
AAGAAAAGACAACGAAGCAA+AGG 0.535441 4_2:-50721544 Msa0604940:CDS
TTCGCGGAGTTAGTCCTCTC+GGG 0.536295 4_2:-50722335 Msa0604940:CDS
GAAAAGCTGGGAAGGGAACA+TGG 0.551231 4_2:-50722241 Msa0604940:CDS
GAAATATCAATGGAGTGGAT+AGG 0.557954 4_2:-50722398 Msa0604940:CDS
ATCTATTGCGAAGCTTGGGA+GGG 0.567095 4_2:-50721408 Msa0604940:CDS
ATAGGCTGATTGCTAAGGTG+GGG 0.576897 4_2:-50722380 Msa0604940:CDS
TAGGCTGATTGCTAAGGTGG+GGG 0.578325 4_2:-50722379 Msa0604940:CDS
CTCGGGAAGAGAATTGCAGT+TGG 0.583302 4_2:-50722318 Msa0604940:CDS
AACTTGGTGCTAAGGCGTTG+CGG 0.593967 4_2:-50722263 Msa0604940:CDS
ACAAGGAAATATCAATGGAG+TGG 0.598845 4_2:-50722403 Msa0604940:CDS
GATGATGAGTCATAACAGAC+AGG 0.599947 4_2:+50721170 None:intergenic
GCGTTGCGGAAAAGCTGGGA+AGG 0.605950 4_2:-50722249 Msa0604940:CDS
ACTTCCCGGAAACTTTGTCA+AGG 0.621117 4_2:-50721489 Msa0604940:CDS
TAAGGCGTTGCGGAAAAGCT+GGG 0.622391 4_2:-50722253 Msa0604940:CDS
GATAGGCTGATTGCTAAGGT+GGG 0.622398 4_2:-50722381 Msa0604940:CDS
CAAGCTTCGCAATAGATGAA+GGG 0.632626 4_2:+50721414 None:intergenic
CGTTGCGGAAAAGCTGGGAA+GGG 0.647668 4_2:-50722248 Msa0604940:CDS
AGTGGATAGGCTGATTGCTA+AGG 0.655516 4_2:-50722385 Msa0604940:CDS
TCTAATGGAGTTAAGCAACA+AGG 0.656865 4_2:-50722420 Msa0604940:CDS
ACTGCAATTCTCTTCCCGAG+AGG 0.722826 4_2:+50722321 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAATAAGTAAATACAAAC+AGG + chr4_2:50721554-50721573 None:intergenic 15.0%
!! AAATCTATAAAGAAAGTTAA+CGG - chr4_2:50722083-50722102 Msa0604940:intron 15.0%
!! TAAAATAAGTAAATACAAAC+AGG + chr4_2:50721554-50721573 None:intergenic 15.0%
!! AAATCTATAAAGAAAGTTAA+CGG - chr4_2:50722083-50722102 Msa0604940:intron 15.0%
!! AGTAAGTTTATTCACAATTT+AGG - chr4_2:50721528-50721547 Msa0604940:CDS 20.0%
!! AGTAAGTTTATTCACAATTT+AGG - chr4_2:50721528-50721547 Msa0604940:CDS 20.0%
!!! TCTTTTGAGAAATTCTCTAA+TGG - chr4_2:50721235-50721254 Msa0604940:three_prime_UTR 25.0%
!! TCAATCAGAAATTTAGTTCA+AGG - chr4_2:50721379-50721398 Msa0604940:exon 25.0%
! TATAAGAAGTGATTATGCAT+AGG + chr4_2:50721723-50721742 None:intergenic 25.0%
!! TTTTAACAAGATTCACTTTC+TGG - chr4_2:50721950-50721969 Msa0604940:intron 25.0%
! TAGTTGTATCATTAGTTAGT+TGG - chr4_2:50722336-50722355 Msa0604940:CDS 25.0%
!! AGCATTAAATACTTTTGTTG+GGG + chr4_2:50722385-50722404 None:intergenic 25.0%
!! GAGCATTAAATACTTTTGTT+GGG + chr4_2:50722386-50722405 None:intergenic 25.0%
!!! TCTTTTGAGAAATTCTCTAA+TGG - chr4_2:50721235-50721254 Msa0604940:three_prime_UTR 25.0%
!! TCAATCAGAAATTTAGTTCA+AGG - chr4_2:50721379-50721398 Msa0604940:exon 25.0%
! TATAAGAAGTGATTATGCAT+AGG + chr4_2:50721723-50721742 None:intergenic 25.0%
!! TTTTAACAAGATTCACTTTC+TGG - chr4_2:50721950-50721969 Msa0604940:intron 25.0%
! TAGTTGTATCATTAGTTAGT+TGG - chr4_2:50722336-50722355 Msa0604940:CDS 25.0%
!! AGCATTAAATACTTTTGTTG+GGG + chr4_2:50722385-50722404 None:intergenic 25.0%
!! GAGCATTAAATACTTTTGTT+GGG + chr4_2:50722386-50722405 None:intergenic 25.0%
GAGAATTTCTCAAAAGAAGT+AGG + chr4_2:50721232-50721251 None:intergenic 30.0%
AAGCAACAAGGAAATATCAA+TGG - chr4_2:50721262-50721281 Msa0604940:three_prime_UTR 30.0%
TTAGTTCAAGGAATTGAACT+TGG - chr4_2:50721391-50721410 Msa0604940:CDS 30.0%
ATAAAGAGACATCCAAATCA+AGG - chr4_2:50721464-50721483 Msa0604940:CDS 30.0%
TAAAGAGACATCCAAATCAA+GGG - chr4_2:50721465-50721484 Msa0604940:CDS 30.0%
!! CTTATTTCTATAAGCACTCT+AGG - chr4_2:50721659-50721678 Msa0604940:CDS 30.0%
! ATTATGCATAGGCTATTTCA+TGG + chr4_2:50721712-50721731 None:intergenic 30.0%
CTTAATTGATACGTTTATCC+AGG - chr4_2:50721785-50721804 Msa0604940:intron 30.0%
TCAAAGTTGTTCAAAAAGAC+AGG + chr4_2:50721811-50721830 None:intergenic 30.0%
AAACAAAGAAAAGTCCAACA+AGG + chr4_2:50721849-50721868 None:intergenic 30.0%
TTACTCTCGTAATCTTTAGA+AGG + chr4_2:50722017-50722036 None:intergenic 30.0%
!!! AATCTTGTTTTCTTCCTTAG+AGG + chr4_2:50722060-50722079 None:intergenic 30.0%
ATAAAGAAAGTTAACGGTGA+TGG - chr4_2:50722089-50722108 Msa0604940:intron 30.0%
AAGCTGAAACTGCAAAAATA+AGG + chr4_2:50722307-50722326 None:intergenic 30.0%
! CGAGCATTAAATACTTTTGT+TGG + chr4_2:50722387-50722406 None:intergenic 30.0%
!!! CATTGCCATGTTTTTTAACT+CGG - chr4_2:50722450-50722469 Msa0604940:CDS 30.0%
CTTAACCGAGTTAAAAAACA+TGG + chr4_2:50722458-50722477 None:intergenic 30.0%
GAGAATTTCTCAAAAGAAGT+AGG + chr4_2:50721232-50721251 None:intergenic 30.0%
AAGCAACAAGGAAATATCAA+TGG - chr4_2:50721262-50721281 Msa0604940:three_prime_UTR 30.0%
TTAGTTCAAGGAATTGAACT+TGG - chr4_2:50721391-50721410 Msa0604940:CDS 30.0%
ATAAAGAGACATCCAAATCA+AGG - chr4_2:50721464-50721483 Msa0604940:CDS 30.0%
TAAAGAGACATCCAAATCAA+GGG - chr4_2:50721465-50721484 Msa0604940:CDS 30.0%
!! CTTATTTCTATAAGCACTCT+AGG - chr4_2:50721659-50721678 Msa0604940:CDS 30.0%
! ATTATGCATAGGCTATTTCA+TGG + chr4_2:50721712-50721731 None:intergenic 30.0%
CTTAATTGATACGTTTATCC+AGG - chr4_2:50721785-50721804 Msa0604940:intron 30.0%
TCAAAGTTGTTCAAAAAGAC+AGG + chr4_2:50721811-50721830 None:intergenic 30.0%
AAACAAAGAAAAGTCCAACA+AGG + chr4_2:50721849-50721868 None:intergenic 30.0%
TTACTCTCGTAATCTTTAGA+AGG + chr4_2:50722017-50722036 None:intergenic 30.0%
!!! AATCTTGTTTTCTTCCTTAG+AGG + chr4_2:50722060-50722079 None:intergenic 30.0%
ATAAAGAAAGTTAACGGTGA+TGG - chr4_2:50722089-50722108 Msa0604940:intron 30.0%
AAGCTGAAACTGCAAAAATA+AGG + chr4_2:50722307-50722326 None:intergenic 30.0%
! CGAGCATTAAATACTTTTGT+TGG + chr4_2:50722387-50722406 None:intergenic 30.0%
!!! CATTGCCATGTTTTTTAACT+CGG - chr4_2:50722450-50722469 Msa0604940:CDS 30.0%
CTTAACCGAGTTAAAAAACA+TGG + chr4_2:50722458-50722477 None:intergenic 30.0%
TCTAATGGAGTTAAGCAACA+AGG - chr4_2:50721250-50721269 Msa0604940:three_prime_UTR 35.0%
ACAAGGAAATATCAATGGAG+TGG - chr4_2:50721267-50721286 Msa0604940:three_prime_UTR 35.0%
GAAATATCAATGGAGTGGAT+AGG - chr4_2:50721272-50721291 Msa0604940:three_prime_UTR 35.0%
AAGAAAAGACAACGAAGCAA+AGG - chr4_2:50722126-50722145 Msa0604940:intron 35.0%
TGAAACCTTGACAAAGTTTC+CGG + chr4_2:50722189-50722208 None:intergenic 35.0%
TCTAATGGAGTTAAGCAACA+AGG - chr4_2:50721250-50721269 Msa0604940:three_prime_UTR 35.0%
ACAAGGAAATATCAATGGAG+TGG - chr4_2:50721267-50721286 Msa0604940:three_prime_UTR 35.0%
GAAATATCAATGGAGTGGAT+AGG - chr4_2:50721272-50721291 Msa0604940:three_prime_UTR 35.0%
AAGAAAAGACAACGAAGCAA+AGG - chr4_2:50722126-50722145 Msa0604940:intron 35.0%
TGAAACCTTGACAAAGTTTC+CGG + chr4_2:50722189-50722208 None:intergenic 35.0%
TAGCAACTAGTAGGTGAAGA+AGG + chr4_2:50721202-50721221 None:intergenic 40.0%
GGCAAAGAATAGCAACTAGT+AGG + chr4_2:50721211-50721230 None:intergenic 40.0%
!! AGGAATTGAACTTGGTGCTA+AGG - chr4_2:50721399-50721418 Msa0604940:CDS 40.0%
CTTAGAAGATGCCCTTGATT+TGG + chr4_2:50721479-50721498 None:intergenic 40.0%
CTTACTGTGCTACGAAATTC+AGG + chr4_2:50721514-50721533 None:intergenic 40.0%
TAAGCACTTGTGAGACAGTT+TGG - chr4_2:50721589-50721608 Msa0604940:CDS 40.0%
AGACAGTTTGGTAGAGCTTA+TGG - chr4_2:50721601-50721620 Msa0604940:CDS 40.0%
TGTCAAACATCAGACCTTGT+TGG - chr4_2:50721832-50721851 Msa0604940:intron 40.0%
! ATGTACTTTTCCTAGGAGTC+TGG + chr4_2:50721980-50721999 None:intergenic 40.0%
! TCACTCGATGTACTTTTCCT+AGG + chr4_2:50721987-50722006 None:intergenic 40.0%
! GTTTTCTTCCTTAGAGGACT+TGG + chr4_2:50722054-50722073 None:intergenic 40.0%
!! AGTTAACGGTGATGGAACTA+TGG - chr4_2:50722097-50722116 Msa0604940:intron 40.0%
! ACGAAGCAAAGGAACTCTTT+TGG - chr4_2:50722137-50722156 Msa0604940:intron 40.0%
GGAAAGAAATCTTCAGAAGC+CGG - chr4_2:50722158-50722177 Msa0604940:intron 40.0%
GAAACCTTGACAAAGTTTCC+GGG + chr4_2:50722188-50722207 None:intergenic 40.0%
TGACAGAAGCAAGTGTTCAA+TGG - chr4_2:50722225-50722244 Msa0604940:intron 40.0%
CAAGCTTCGCAATAGATGAA+GGG + chr4_2:50722259-50722278 None:intergenic 40.0%
CTTCATCTATTGCGAAGCTT+GGG - chr4_2:50722258-50722277 Msa0604940:CDS 40.0%
GTCATAACAGACAGGATCTT+TGG + chr4_2:50722495-50722514 None:intergenic 40.0%
TAGCAACTAGTAGGTGAAGA+AGG + chr4_2:50721202-50721221 None:intergenic 40.0%
GGCAAAGAATAGCAACTAGT+AGG + chr4_2:50721211-50721230 None:intergenic 40.0%
!! AGGAATTGAACTTGGTGCTA+AGG - chr4_2:50721399-50721418 Msa0604940:CDS 40.0%
CTTAGAAGATGCCCTTGATT+TGG + chr4_2:50721479-50721498 None:intergenic 40.0%
CTTACTGTGCTACGAAATTC+AGG + chr4_2:50721514-50721533 None:intergenic 40.0%
TAAGCACTTGTGAGACAGTT+TGG - chr4_2:50721589-50721608 Msa0604940:CDS 40.0%
AGACAGTTTGGTAGAGCTTA+TGG - chr4_2:50721601-50721620 Msa0604940:CDS 40.0%
TGTCAAACATCAGACCTTGT+TGG - chr4_2:50721832-50721851 Msa0604940:intron 40.0%
! ATGTACTTTTCCTAGGAGTC+TGG + chr4_2:50721980-50721999 None:intergenic 40.0%
! TCACTCGATGTACTTTTCCT+AGG + chr4_2:50721987-50722006 None:intergenic 40.0%
! GTTTTCTTCCTTAGAGGACT+TGG + chr4_2:50722054-50722073 None:intergenic 40.0%
!! AGTTAACGGTGATGGAACTA+TGG - chr4_2:50722097-50722116 Msa0604940:intron 40.0%
! ACGAAGCAAAGGAACTCTTT+TGG - chr4_2:50722137-50722156 Msa0604940:intron 40.0%
GGAAAGAAATCTTCAGAAGC+CGG - chr4_2:50722158-50722177 Msa0604940:intron 40.0%
GAAACCTTGACAAAGTTTCC+GGG + chr4_2:50722188-50722207 None:intergenic 40.0%
TGACAGAAGCAAGTGTTCAA+TGG - chr4_2:50722225-50722244 Msa0604940:intron 40.0%
CAAGCTTCGCAATAGATGAA+GGG + chr4_2:50722259-50722278 None:intergenic 40.0%
CTTCATCTATTGCGAAGCTT+GGG - chr4_2:50722258-50722277 Msa0604940:CDS 40.0%
GTCATAACAGACAGGATCTT+TGG + chr4_2:50722495-50722514 None:intergenic 40.0%
AGTGGATAGGCTGATTGCTA+AGG - chr4_2:50721285-50721304 Msa0604940:three_prime_UTR 45.0%
GATAGGCTGATTGCTAAGGT+GGG - chr4_2:50721289-50721308 Msa0604940:three_prime_UTR 45.0%
ATAGGCTGATTGCTAAGGTG+GGG - chr4_2:50721290-50721309 Msa0604940:three_prime_UTR 45.0%
GTTGTTCAAAAAGACAGGCC+TGG + chr4_2:50721806-50721825 None:intergenic 45.0%
AACACCAACCAAGTCCTCTA+AGG - chr4_2:50722043-50722062 Msa0604940:intron 45.0%
! TCTTCCTTAGAGGACTTGGT+TGG + chr4_2:50722050-50722069 None:intergenic 45.0%
ACTTCCCGGAAACTTTGTCA+AGG - chr4_2:50722181-50722200 Msa0604940:intron 45.0%
CCAAGCTTCGCAATAGATGA+AGG + chr4_2:50722260-50722279 None:intergenic 45.0%
CCTTCATCTATTGCGAAGCT+TGG - chr4_2:50722257-50722276 Msa0604940:CDS 45.0%
ATCTATTGCGAAGCTTGGGA+GGG - chr4_2:50722262-50722281 Msa0604940:CDS 45.0%
AGTGGATAGGCTGATTGCTA+AGG - chr4_2:50721285-50721304 Msa0604940:three_prime_UTR 45.0%
GATAGGCTGATTGCTAAGGT+GGG - chr4_2:50721289-50721308 Msa0604940:three_prime_UTR 45.0%
ATAGGCTGATTGCTAAGGTG+GGG - chr4_2:50721290-50721309 Msa0604940:three_prime_UTR 45.0%
GTTGTTCAAAAAGACAGGCC+TGG + chr4_2:50721806-50721825 None:intergenic 45.0%
AACACCAACCAAGTCCTCTA+AGG - chr4_2:50722043-50722062 Msa0604940:intron 45.0%
! TCTTCCTTAGAGGACTTGGT+TGG + chr4_2:50722050-50722069 None:intergenic 45.0%
ACTTCCCGGAAACTTTGTCA+AGG - chr4_2:50722181-50722200 Msa0604940:intron 45.0%
CCAAGCTTCGCAATAGATGA+AGG + chr4_2:50722260-50722279 None:intergenic 45.0%
CCTTCATCTATTGCGAAGCT+TGG - chr4_2:50722257-50722276 Msa0604940:CDS 45.0%
ATCTATTGCGAAGCTTGGGA+GGG - chr4_2:50722262-50722281 Msa0604940:CDS 45.0%
GGATAGGCTGATTGCTAAGG+TGG - chr4_2:50721288-50721307 Msa0604940:three_prime_UTR 50.0%
TAGGCTGATTGCTAAGGTGG+GGG - chr4_2:50721291-50721310 Msa0604940:three_prime_UTR 50.0%
ACTGCAATTCTCTTCCCGAG+AGG + chr4_2:50721352-50721371 None:intergenic 50.0%
CTCGGGAAGAGAATTGCAGT+TGG - chr4_2:50721352-50721371 Msa0604940:three_prime_UTR 50.0%
!! AACTTGGTGCTAAGGCGTTG+CGG - chr4_2:50721407-50721426 Msa0604940:CDS 50.0%
!! TAAGGCGTTGCGGAAAAGCT+GGG - chr4_2:50721417-50721436 Msa0604940:CDS 50.0%
GAAAAGCTGGGAAGGGAACA+TGG - chr4_2:50721429-50721448 Msa0604940:CDS 50.0%
TTCTGGTGTTCCAGACTCCT+AGG - chr4_2:50721967-50721986 Msa0604940:intron 50.0%
GACAAAGTTTCCGGGAAGTC+CGG + chr4_2:50722180-50722199 None:intergenic 50.0%
CATCTATTGCGAAGCTTGGG+AGG - chr4_2:50722261-50722280 Msa0604940:CDS 50.0%
GGATAGGCTGATTGCTAAGG+TGG - chr4_2:50721288-50721307 Msa0604940:three_prime_UTR 50.0%
TAGGCTGATTGCTAAGGTGG+GGG - chr4_2:50721291-50721310 Msa0604940:three_prime_UTR 50.0%
ACTGCAATTCTCTTCCCGAG+AGG + chr4_2:50721352-50721371 None:intergenic 50.0%
CTCGGGAAGAGAATTGCAGT+TGG - chr4_2:50721352-50721371 Msa0604940:three_prime_UTR 50.0%
!! AACTTGGTGCTAAGGCGTTG+CGG - chr4_2:50721407-50721426 Msa0604940:CDS 50.0%
!! TAAGGCGTTGCGGAAAAGCT+GGG - chr4_2:50721417-50721436 Msa0604940:CDS 50.0%
GAAAAGCTGGGAAGGGAACA+TGG - chr4_2:50721429-50721448 Msa0604940:CDS 50.0%
TTCTGGTGTTCCAGACTCCT+AGG - chr4_2:50721967-50721986 Msa0604940:intron 50.0%
GACAAAGTTTCCGGGAAGTC+CGG + chr4_2:50722180-50722199 None:intergenic 50.0%
CATCTATTGCGAAGCTTGGG+AGG - chr4_2:50722261-50722280 Msa0604940:CDS 50.0%
GTTCGCGGAGTTAGTCCTCT+CGG - chr4_2:50721334-50721353 Msa0604940:three_prime_UTR 55.0%
TTCGCGGAGTTAGTCCTCTC+GGG - chr4_2:50721335-50721354 Msa0604940:three_prime_UTR 55.0%
!! CTAAGGCGTTGCGGAAAAGC+TGG - chr4_2:50721416-50721435 Msa0604940:CDS 55.0%
CGTTGCGGAAAAGCTGGGAA+GGG - chr4_2:50721422-50721441 Msa0604940:CDS 55.0%
TCTTCAGAAGCCGGACTTCC+CGG - chr4_2:50722167-50722186 Msa0604940:intron 55.0%
GTTCGCGGAGTTAGTCCTCT+CGG - chr4_2:50721334-50721353 Msa0604940:three_prime_UTR 55.0%
TTCGCGGAGTTAGTCCTCTC+GGG - chr4_2:50721335-50721354 Msa0604940:three_prime_UTR 55.0%
!! CTAAGGCGTTGCGGAAAAGC+TGG - chr4_2:50721416-50721435 Msa0604940:CDS 55.0%
CGTTGCGGAAAAGCTGGGAA+GGG - chr4_2:50721422-50721441 Msa0604940:CDS 55.0%
TCTTCAGAAGCCGGACTTCC+CGG - chr4_2:50722167-50722186 Msa0604940:intron 55.0%
GCTGATTGCTAAGGTGGGGG+TGG - chr4_2:50721294-50721313 Msa0604940:three_prime_UTR 60.0%
GATTGCTAAGGTGGGGGTGG+TGG - chr4_2:50721297-50721316 Msa0604940:three_prime_UTR 60.0%
!! TAAGGTGGGGGTGGTGGAGA+CGG - chr4_2:50721303-50721322 Msa0604940:three_prime_UTR 60.0%
!! GAGACGGGAAAGGGTGTTCG+CGG - chr4_2:50721319-50721338 Msa0604940:three_prime_UTR 60.0%
GCGTTGCGGAAAAGCTGGGA+AGG - chr4_2:50721421-50721440 Msa0604940:CDS 60.0%
GCTGATTGCTAAGGTGGGGG+TGG - chr4_2:50721294-50721313 Msa0604940:three_prime_UTR 60.0%
GATTGCTAAGGTGGGGGTGG+TGG - chr4_2:50721297-50721316 Msa0604940:three_prime_UTR 60.0%
!! TAAGGTGGGGGTGGTGGAGA+CGG - chr4_2:50721303-50721322 Msa0604940:three_prime_UTR 60.0%
!! GAGACGGGAAAGGGTGTTCG+CGG - chr4_2:50721319-50721338 Msa0604940:three_prime_UTR 60.0%
GCGTTGCGGAAAAGCTGGGA+AGG - chr4_2:50721421-50721440 Msa0604940:CDS 60.0%
!! AAGGTGGGGGTGGTGGAGAC+GGG - chr4_2:50721304-50721323 Msa0604940:three_prime_UTR 65.0%
!! GGGGTGGTGGAGACGGGAAA+GGG - chr4_2:50721310-50721329 Msa0604940:three_prime_UTR 65.0%
!! AAGGTGGGGGTGGTGGAGAC+GGG - chr4_2:50721304-50721323 Msa0604940:three_prime_UTR 65.0%
!! GGGGTGGTGGAGACGGGAAA+GGG - chr4_2:50721310-50721329 Msa0604940:three_prime_UTR 65.0%
!! GGGGGTGGTGGAGACGGGAA+AGG - chr4_2:50721309-50721328 Msa0604940:three_prime_UTR 70.0%
!! GGGGGTGGTGGAGACGGGAA+AGG - chr4_2:50721309-50721328 Msa0604940:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 50721178 50722514 50721178 ID=Msa0604940;Name=Msa0604940
chr4_2 mRNA 50721178 50722514 50721178 ID=Msa0604940-mRNA-1;Parent=Msa0604940;Name=Msa0604940-mRNA-1;_AED=0.22;_eAED=0.22;_QI=0|0|0.5|1|1|1|2|213|198
chr4_2 exon 50721178 50721711 50721178 ID=Msa0604940-mRNA-1:exon:12036;Parent=Msa0604940-mRNA-1
chr4_2 exon 50722239 50722514 50722239 ID=Msa0604940-mRNA-1:exon:12035;Parent=Msa0604940-mRNA-1
chr4_2 CDS 50722239 50722514 50722239 ID=Msa0604940-mRNA-1:cds;Parent=Msa0604940-mRNA-1
chr4_2 CDS 50721391 50721711 50721391 ID=Msa0604940-mRNA-1:cds;Parent=Msa0604940-mRNA-1
chr4_2 three_prime_UTR 50721178 50721390 50721178 ID=Msa0604940-mRNA-1:three_prime_utr;Parent=Msa0604940-mRNA-1
Gene Sequence

>Msa0604940

TTGAATTCAAGAGCTATTGTTCCTTCTTCACCTACTAGTTGCTATTCTTTGCCTACTTCTTTTGAGAAATTCTCTAATGGAGTTAAGCAACAAGGAAATATCAATGGAGTGGATAGGCTGATTGCTAAGGTGGGGGTGGTGGAGACGGGAAAGGGTGTTCGCGGAGTTAGTCCTCTCGGGAAGAGAATTGCAGTTGGAAATTCAATCAGAAATTTAGTTCAAGGAATTGAACTTGGTGCTAAGGCGTTGCGGAAAAGCTGGGAAGGGAACATGGAAACTCCTAGGAAAAGTACATCGAGTGAGAAGTTTCCTTCTAAAGATTACGAGAGTAAGACACAAACACCAACCAAGTCCTCTAAGGAAGAAAACAAGATTCAAAAATCTATAAAGAAAGTTAACGGTGATGGAACTATGGAAGAACAAGAAAAGACAACGAAGCAAAGGAACTCTTTTGGAAAGAAATCTTCAGAAGCCGGACTTCCCGGAAACTTTGTCAAGGTTTCAGTAAATAGTAAAAAAGTGACAGAAGCAAGTGTTCAATGGACTTCACTCCCTTCATCTATTGCGAAGCTTGGGAGGGTATATATCATCTTATAA
Protein sequence

>Msa0604940

LNSRAIVPSSPTSCYSLPTSFEKFSNGVKQQGNINGVDRLIAKVGVVETGKGVRGVSPLGKRIAVGNSIRNLVQGIELGAKALRKSWEGNMETPRKSTSSEKFPSKDYESKTQTPTKSSKEENKIQKSIKKVNGDGTMEEQEKTTKQRNSFGKKSSEAGLPGNFVKVSVNSKKVTEASVQWTSLPSSIAKLGRVYIIL*