Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0001960 | Msa0945490 | 0.816568 | 5.172476e-52 | -8.615850e-47 |
| Msa0030990 | Msa0945490 | 0.823019 | 1.734881e-53 | -8.615850e-47 |
| Msa0032920 | Msa0945490 | -0.807562 | 4.756982e-50 | -8.615850e-47 |
| Msa0036890 | Msa0945490 | 0.810837 | 9.451300e-51 | -8.615850e-47 |
| Msa0051020 | Msa0945490 | 0.802954 | 4.391128e-49 | -8.615850e-47 |
| Msa0052870 | Msa0945490 | 0.801231 | 9.927568e-49 | -8.615850e-47 |
| Msa0060760 | Msa0945490 | 0.810118 | 1.351178e-50 | -8.615850e-47 |
| Msa0061340 | Msa0945490 | 0.814662 | 1.374312e-51 | -8.615850e-47 |
| Msa0074600 | Msa0945490 | 0.828581 | 8.304190e-55 | -8.615850e-47 |
| Msa0084970 | Msa0945490 | 0.819144 | 1.354767e-52 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0945490 | MtrunA17_Chr4g0033751 | 96.011 | 727 | 27 | 1 | 1 | 725 | 1 | 727 | 0.0 | 1424 |
| Msa0945490 | MtrunA17_Chr2g0329581 | 62.681 | 761 | 209 | 17 | 1 | 718 | 1 | 729 | 0.0 | 817 |
| Msa0945490 | MtrunA17_Chr6g0475401 | 63.025 | 119 | 44 | 0 | 1 | 119 | 1 | 119 | 8.89e-48 | 177 |
| Msa0945490 | MtrunA17_Chr6g0475401 | 35.833 | 120 | 60 | 3 | 608 | 712 | 412 | 529 | 3.08e-12 | 69.7 |
| Msa0945490 | MtrunA17_Chr5g0413621 | 57.025 | 121 | 51 | 1 | 1 | 120 | 1 | 121 | 1.52e-41 | 160 |
| Msa0945490 | MtrunA17_Chr4g0062781 | 56.667 | 120 | 52 | 0 | 1 | 120 | 1 | 120 | 3.61e-40 | 157 |
| Msa0945490 | MtrunA17_Chr2g0309091 | 31.680 | 363 | 196 | 13 | 369 | 711 | 254 | 584 | 7.57e-37 | 146 |
| Msa0945490 | MtrunA17_Chr2g0309091 | 46.825 | 126 | 61 | 2 | 1 | 124 | 1 | 122 | 1.33e-26 | 115 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0945490 | AT1G08760.1 | 51.747 | 744 | 298 | 17 | 1 | 706 | 1 | 721 | 0.0 | 677 |
| Msa0945490 | AT3G14170.1 | 61.345 | 119 | 46 | 0 | 1 | 119 | 1 | 119 | 2.81e-45 | 170 |
| Msa0945490 | AT3G14170.1 | 35.338 | 133 | 69 | 3 | 594 | 712 | 374 | 503 | 4.95e-13 | 72.8 |
| Msa0945490 | AT3G14170.1 | 34.831 | 89 | 58 | 0 | 384 | 472 | 250 | 338 | 1.67e-12 | 70.9 |
| Msa0945490 | AT3G14170.2 | 61.345 | 119 | 46 | 0 | 1 | 119 | 1 | 119 | 2.94e-45 | 169 |
| Msa0945490 | AT3G14170.2 | 35.338 | 133 | 69 | 3 | 594 | 712 | 375 | 504 | 5.32e-13 | 72.4 |
| Msa0945490 | AT3G14170.2 | 34.831 | 89 | 58 | 0 | 384 | 472 | 251 | 339 | 1.82e-12 | 70.9 |
| Msa0945490 | AT4G13370.1 | 58.400 | 125 | 52 | 0 | 1 | 125 | 1 | 125 | 3.07e-42 | 164 |
| Msa0945490 | AT4G13370.1 | 28.135 | 327 | 160 | 6 | 382 | 707 | 413 | 665 | 2.24e-23 | 105 |
| Msa0945490 | AT1G23790.1 | 57.377 | 122 | 51 | 1 | 1 | 121 | 1 | 122 | 3.53e-41 | 158 |
Find 161 sgRNAs with CRISPR-Local
Find 261 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATGGCAAATCTAGTTCC+TGG | 0.348238 | 6_3:+24277612 | Msa0945490:exon |
| AAATCATCCGCGCACTATTC+AGG | 0.311812 | 6_3:-24280534 | None:intergenic |
| AAATCTATAAAGAAAGTTAA+CGG | 0.457905 | 6_3:+24279090 | Msa0945490:CDS |
| AAATCTCCACCTGCTAGTGC+TGG | 0.505213 | 6_3:-24277726 | None:intergenic |
| AAATGGCAAATCTAGTTCCT+GGG | 0.508006 | 6_3:+24277613 | Msa0945490:exon |
| AACAATAAATGTGAAATGTG+AGG | 0.588377 | 6_3:+24281527 | Msa0945490:three_prime_UTR |
| AACACAGACATAAAAGTTGG+TGG | 0.601218 | 6_3:+24277663 | Msa0945490:CDS |
| AACACCAACCAAGTCCTCTA+AGG | 0.411554 | 6_3:+24279050 | Msa0945490:CDS |
| AACAGAAAAGGATGAGAGAT+TGG | 0.439259 | 6_3:-24280689 | None:intergenic |
| AACGATTGGTTGGATGAGAT+AGG | 0.546451 | 6_3:+24281135 | Msa0945490:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGGTAGACTATGATAATCA+TGG | - | chr6_3:24277135-24277154 | None:intergenic | 30.0% | |
| AACTAGAAACTAGAAACTGA+TGG | - | chr6_3:24277451-24277470 | None:intergenic | 30.0% | |
| ACTCTTCTAATACTCAAATG+GGG | - | chr6_3:24277563-24277582 | None:intergenic | 30.0% | |
| ATGAACACAGACATAAAAGT+TGG | + | chr6_3:24277660-24277679 | Msa0945490:CDS | 30.0% | |
| TTAGATCATCATGTTCATCA+GGG | - | chr6_3:24277815-24277834 | None:intergenic | 30.0% | |
| ATTAGATCATCATGTTCATC+AGG | - | chr6_3:24277816-24277835 | None:intergenic | 30.0% | |
| TTAGTTGCAACAATATCTTC+AGG | - | chr6_3:24277960-24277979 | None:intergenic | 30.0% | |
| GAGAATTTCTCAAAAGAAGT+AGG | - | chr6_3:24278239-24278258 | None:intergenic | 30.0% | |
| AAGCAACAAGGAAATATCAA+TGG | + | chr6_3:24278269-24278288 | Msa0945490:CDS | 30.0% | |
| TTAGTTCAAGGAATTGAACT+TGG | + | chr6_3:24278398-24278417 | Msa0945490:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_3 | gene | 24277122 | 24281609 | 24277122 | ID=Msa0945490;Name=Msa0945490 |
| chr6_3 | mRNA | 24277122 | 24281609 | 24277122 | ID=Msa0945490-mRNA-1;Parent=Msa0945490;Name=Msa0945490-mRNA-1;_AED=0.10;_eAED=0.10;_QI=493|1|1|1|1|1|4|295|725 |
| chr6_3 | exon | 24277122 | 24278535 | 24277122 | ID=Msa0945490-mRNA-1:exon:6937;Parent=Msa0945490-mRNA-1 |
| chr6_3 | exon | 24278988 | 24279290 | 24278988 | ID=Msa0945490-mRNA-1:exon:6938;Parent=Msa0945490-mRNA-1 |
| chr6_3 | exon | 24279778 | 24279869 | 24279778 | ID=Msa0945490-mRNA-1:exon:6939;Parent=Msa0945490-mRNA-1 |
| chr6_3 | exon | 24280453 | 24281609 | 24280453 | ID=Msa0945490-mRNA-1:exon:6940;Parent=Msa0945490-mRNA-1 |
| chr6_3 | five_prime_UTR | 24277122 | 24277614 | 24277122 | ID=Msa0945490-mRNA-1:five_prime_utr;Parent=Msa0945490-mRNA-1 |
| chr6_3 | CDS | 24277615 | 24278535 | 24277615 | ID=Msa0945490-mRNA-1:cds;Parent=Msa0945490-mRNA-1 |
| chr6_3 | CDS | 24278988 | 24279290 | 24278988 | ID=Msa0945490-mRNA-1:cds;Parent=Msa0945490-mRNA-1 |
| chr6_3 | CDS | 24279778 | 24279869 | 24279778 | ID=Msa0945490-mRNA-1:cds;Parent=Msa0945490-mRNA-1 |
| chr6_3 | CDS | 24280453 | 24281314 | 24280453 | ID=Msa0945490-mRNA-1:cds;Parent=Msa0945490-mRNA-1 |
| chr6_3 | three_prime_UTR | 24281315 | 24281609 | 24281315 | ID=Msa0945490-mRNA-1:three_prime_utr;Parent=Msa0945490-mRNA-1 |
| Gene Sequence |
>Msa0945490 ATGGCAAATCTAGTTCCTGGGGTGCTACTAAAGTTAATGCAGCACATGAACACAGACATAAAAGTTGGTGGTGAACATAGATCATCATTGTTACAAGTTGTGAGCATTGTTCCAGCACTAGCAGGTGGAGATTTGTTTACAAATCAAGGCTTTTATGTTAAGGTTTCTGATTCATCACATGCTACTTATGTCTCTCTCCCTGATGAACATGATGATCTAATCCTTAGTGACAAGATTCAGTTAGGCCAGTTTGTTTTTGTTGATCGGTTTGAAGCTGCTTCGCCTGTTCCGATTCTTCGTGGTGTTAGGCCTGTTCCTGGGAGACATGCTTGTGTTGGAACACCTGAAGATATTGTTGCAACTAACTCACTTGGTTTTCTTAGCAATGCTTCTGAGGTTAAGGTTAAGAGGAATAGTGTTTGTTCCAAGTCCTCTCCAATGAAAGTGTTGGAGAATCATCAAGAACAAGTAGATAAGAAATCAATGGTTTTAGGTAGATGTAAATCTCAGACAGCAAAAGATTCTGTTGTTGATTTTGTGAAGAAGGAAAAGTTAACAAGATTAAAGTCATTGAATTCAAGAGCTATTGTTCCTTCTTCACCTACTAGTTGCTATTCTTTGCCTACTTCTTTTGAGAAATTCTCTAATGGAGTTAAGCAACAAGGAAATATCAATGGAGTGGATAGGCTGATTGCTAAGGTGGGGGTGGTGGAGACGGGAAAGGGTGTTCGCGGAGTTAGTCCTCTCGGGAAGAGAATTGCAGTTGGAAATTCAATCAGAAATTTAGTTCAAGGAATTGAACTTGGTGCTAAGGCGTTGCGGAAAAGCTGGGAAGGGAACATGGAAGTTAAGAATAATAAAGAGACATCCAAATCAAGGGCATCTTCTAAGTGTGATTCTAAGCCTGAATTTCGTAGCACAACTCCTAGGAAAAGTACATCGAGTGAGAAGTTTCCTTCTAAAGATTACGAGAGTAAGACACAAACACCAACCAAGTCCTCTAAGGAAGAAAACAAGATTCAAAAATCTATAAAGAAAGTTAACGGTGATGGAACTATGGAAGAACAAGAAAAGACAACGAAGCAAAGGAACTCTTTTGGAAAGAAATCTTCAGAAGCCGGACTTCCCGGAAACTTTGTCAAGGTTTCAGTAAATAGTAAAAAAGTGACAGAAGCAAGTGTTCAATGGACTTCACTCCCTTCATCTATTGCGAAGCTTGGGAGGGAAGTAATGAAGCACAGAGATGCAGCACAGATGGCAGCAACTGAGGCTATGCAAGAGGCTGCTACTGCTGATAGTTTGCTACAATGTCTTAGTGTGTATGCAGAGCTAAGTAATTCTGCCAAGGAACATAACCCTCAGAATACAATTGATCAGTTTTTAACGCTTCACACTAGCCTGAATAGTGCGCGGATGATTTCAGATTCCCTTTCTAAAACCATTCCAGATGGTTCATCTCCAGATAATGAAAGAATCACAACAGAAGAAGCACTAAAACTCAAATCAGATAGACAAAAACTTGCAGCTTCTTGGGTCCAAGCTGCCTTATCCACCAATCTCTCATCCTTTTCTGTTTATAACCGAGAGCCTCAATTGTCCAAGCTTCCTGTTTCAACTACAAGTAATTCTCAAAATCAAAAGATTGTTCTTGGAAGTAAACCAATTTTAGTCCTCGAAAATTCAAGAGAAGACGCTTCATTGAAAGCTCATGGAAAAACTCGTCCAGCAGTAGCTAATTCCAAACAAGCCTTACAAGGAACTCCGCGCAAACAAGGTGATGCAATATCAAATCCAAAAAAGCAACTAGTCCAACAACCACTCCAAGAGTGGATTAAAGGAAATGACCTTGATGCGGCGGTTAATTTAGCCGACATGCTGCAATTGCGATCGCGAGACTGGTTTTTATTGTTTGTTGAGAAATTTTTGGACAGTGATGGAGACATTGGTTTGTCAAACAATGGTCAAATAGCAGGCATGCTCACTCAACTTAAAAGCGTCAACGATTGGTTGGATGAGATAGGATCGAGTAAAAATGAGGGGGAATCATGTCAGATACCGGTAGAGACAATTGATAGACTCAGGAAGAAGATATACGAATATCTTCTTACACATGTTGAATCTGCTGCTGCTGCCCTTACTTCTGACTCACAATCATCAGAGATCAAAGGCAAAAAGTGA |
| Protein sequence |
>Msa0945490 MANLVPGVLLKLMQHMNTDIKVGGEHRSSLLQVVSIVPALAGGDLFTNQGFYVKVSDSSHATYVSLPDEHDDLILSDKIQLGQFVFVDRFEAASPVPILRGVRPVPGRHACVGTPEDIVATNSLGFLSNASEVKVKRNSVCSKSSPMKVLENHQEQVDKKSMVLGRCKSQTAKDSVVDFVKKEKLTRLKSLNSRAIVPSSPTSCYSLPTSFEKFSNGVKQQGNINGVDRLIAKVGVVETGKGVRGVSPLGKRIAVGNSIRNLVQGIELGAKALRKSWEGNMEVKNNKETSKSRASSKCDSKPEFRSTTPRKSTSSEKFPSKDYESKTQTPTKSSKEENKIQKSIKKVNGDGTMEEQEKTTKQRNSFGKKSSEAGLPGNFVKVSVNSKKVTEASVQWTSLPSSIAKLGREVMKHRDAAQMAATEAMQEAATADSLLQCLSVYAELSNSAKEHNPQNTIDQFLTLHTSLNSARMISDSLSKTIPDGSSPDNERITTEEALKLKSDRQKLAASWVQAALSTNLSSFSVYNREPQLSKLPVSTTSNSQNQKIVLGSKPILVLENSREDASLKAHGKTRPAVANSKQALQGTPRKQGDAISNPKKQLVQQPLQEWIKGNDLDAAVNLADMLQLRSRDWFLLFVEKFLDSDGDIGLSNNGQIAGMLTQLKSVNDWLDEIGSSKNEGESCQIPVETIDRLRKKIYEYLLTHVESAAAALTSDSQSSEIKGKK* |
