AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0225680

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0225680 MtrunA17_Chr2g0331991 98.875 711 7 1 1 710 1 711 0.0 1434
Msa0225680 MtrunA17_Chr4g0004211 59.181 708 205 13 2 707 32 657 0.0 790
Msa0225680 MtrunA17_Chr4g0034861 60.088 684 211 12 31 710 9 634 0.0 739
Msa0225680 MtrunA17_Chr3g0141921 31.026 419 201 12 298 708 123 461 1.10e-37 146
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0225680 AT3G15940.2 61.919 688 248 7 32 708 12 696 0.0 808
Msa0225680 AT3G15940.1 61.919 688 248 7 32 708 12 696 0.0 808
Msa0225680 AT1G52420.1 60.347 691 242 12 24 708 5 669 0.0 800
Msa0225680 AT1G19710.1 31.296 409 190 15 305 703 134 461 3.81e-36 142
Msa0225680 AT1G75420.2 42.384 151 83 3 553 703 219 365 9.07e-23 101
Msa0225680 AT1G75420.3 42.384 151 83 3 553 703 278 424 1.10e-22 102
Msa0225680 AT1G75420.1 42.384 151 83 3 553 703 304 450 1.40e-22 102

Find 178 sgRNAs with CRISPR-Local

Find 321 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGGTTCTCGGAACGGATTC+TGG 0.131653 2_1:+75833661 Msa0225680:intron
GATCTCCTGCTCTTCTTCTT+TGG 0.146647 2_1:-75830097 None:intergenic
AAGCAATAGCTGGCCCTTTC+CGG 0.189848 2_1:-75831855 None:intergenic
CTATTGGTTTGAGGAATTTC+TGG 0.194960 2_1:-75830193 None:intergenic
ACGACCTACCTGAGAGTTTA+TGG 0.200248 2_1:-75832365 None:intergenic
ATAATCCACCTTGTTACTTT+TGG 0.226187 2_1:-75832230 None:intergenic
AAGGAATGTGTATTTCTAAA+AGG 0.229381 2_1:+75829525 Msa0225680:five_prime_UTR
ATCTCCTGCTCTTCTTCTTT+GGG 0.236327 2_1:-75830096 None:intergenic
CTCAACAGAAGATAGGATTT+TGG 0.248236 2_1:+75830246 Msa0225680:CDS
CAGCAATAGAGGTGAATTTC+AGG 0.300352 2_1:+75829610 Msa0225680:CDS
GTTCAAGATTGAAGCATTGT+AGG 0.311791 2_1:+75829478 Msa0225680:five_prime_UTR
GGGACTTTGCACGCCTTGTT+TGG 0.322335 2_1:+75830311 Msa0225680:CDS
AGCAATAGCTGGCCCTTTCC+GGG 0.327800 2_1:-75831854 None:intergenic
CCATTTGCTTCCTTGCCAAC+TGG 0.333918 2_1:-75833792 None:intergenic
AATGAGACGGTTAGAAATAA+AGG 0.341557 2_1:+75829995 Msa0225680:CDS
GCATCTACGCCGGTATTTCT+TGG 0.344521 2_1:-75829917 None:intergenic
GGCTGCTTCTAATTACCCTC+TGG 0.345486 2_1:+75829816 Msa0225680:CDS
AAAGTTCTCATTGGTTCTGT+TGG 0.360825 2_1:+75832205 Msa0225680:CDS
TCCACTCTCAATGTTGGAAT+TGG 0.362265 2_1:+75830381 Msa0225680:CDS
AGCAATGGCATTTGGTCTTC+CGG 0.371847 2_1:+75833015 Msa0225680:CDS
ACAGACAAAGGAACAGTTGC+AGG 0.371888 2_1:-75831665 None:intergenic
AGGAAAGAAGGAAGAACTTT+TGG 0.372630 2_1:+75829755 Msa0225680:CDS
GGGGATAGAAGGCAGGAAAA+AGG 0.376967 2_1:+75833813 Msa0225680:CDS
GACGGAGTGTTAAGTGTAGA+AGG 0.388857 2_1:-75831719 None:intergenic
GGATTACTCGTCGGTCCATT+TGG 0.392207 2_1:+75830223 Msa0225680:CDS
TTTGTCTGTTAATGATGAAC+TGG 0.392335 2_1:+75831679 Msa0225680:CDS
AGAATCGGTCCGAAAAGAAA+TGG 0.395205 2_1:+75831784 Msa0225680:CDS
TGGAGTCCTCAGAAACGTTC+CGG 0.397254 2_1:+75830271 Msa0225680:CDS
TTCCCTGCTGCACGTCCAAT+TGG 0.397411 2_1:-75833730 None:intergenic
ATTACCCTCTGGGCTTATCT+TGG 0.397833 2_1:+75829827 Msa0225680:CDS
TTGTTCTTAATTTCCACAAT+TGG 0.400041 2_1:-75830151 None:intergenic
CAGGGACTTGGAGAGACATT+TGG 0.403494 2_1:+75832977 Msa0225680:intron
GAGGCAACCTGTGTTGTGGC+TGG 0.406876 2_1:-75832316 None:intergenic
TGTTGAGGAAAGGAATATAA+TGG 0.407641 2_1:+75829580 Msa0225680:CDS
ATCTTATGGGAGCAGGTTCT+CGG 0.410753 2_1:+75833649 Msa0225680:intron
ACTATAGAAGCAATGGCATT+TGG 0.411383 2_1:+75833007 Msa0225680:CDS
CATCCAATTGGACGTGCAGC+AGG 0.414855 2_1:+75833727 Msa0225680:CDS
CCAGTTGGCAAGGAAGCAAA+TGG 0.419877 2_1:+75833792 Msa0225680:CDS
ATAGTTGAGAACAATGTTAC+AGG 0.420330 2_1:+75833697 Msa0225680:CDS
ACAGGTCTTCTTCATCCAAT+TGG 0.421060 2_1:+75833715 Msa0225680:CDS
GAGTAATCCATACGTACTAT+TGG 0.421436 2_1:-75830209 None:intergenic
TTGTTACCATTGTTGAAAGA+TGG 0.422929 2_1:+75831998 Msa0225680:CDS
AGAGCTAGCTAGTCACAAAT+TGG 0.429410 2_1:-75834001 None:intergenic
AAGGCTGATCTTGTAATTGC+TGG 0.429776 2_1:+75830541 Msa0225680:CDS
AATTTGTTGAGGTTATTGTT+AGG 0.429920 2_1:+75833872 Msa0225680:CDS
CATTTGGCTCAACAGAAGAT+AGG 0.430286 2_1:+75830239 Msa0225680:CDS
CTATCTTCTGTTGAGCCAAA+TGG 0.432141 2_1:-75830238 None:intergenic
AGGTTCTCGGAACGGATTCT+GGG 0.432623 2_1:+75833662 Msa0225680:intron
CCTGCTTAAAGAATTGTTTG+AGG 0.438712 2_1:-75832041 None:intergenic
CAATCTCTCAAAGTTCTCAT+TGG 0.442203 2_1:+75832196 Msa0225680:CDS
CCATGACCCACCAAGCAACT+TGG 0.444595 2_1:-75831508 None:intergenic
AAAGTTCTTCCTTCTTTCCT+CGG 0.444755 2_1:-75829752 None:intergenic
AGGAAGCAAATGGGGATAGA+AGG 0.445303 2_1:+75833802 Msa0225680:CDS
AAATGTAGCCTTGGCAAAGA+AGG 0.452522 2_1:+75830036 Msa0225680:CDS
GGAGTCCTCAGAAACGTTCC+GGG 0.453244 2_1:+75830272 Msa0225680:CDS
CCTCAAACAATTCTTTAAGC+AGG 0.454282 2_1:+75832041 Msa0225680:CDS
TTCCACGAGCTTTCCATGAC+CGG 0.456509 2_1:+75830355 Msa0225680:CDS
TTTCAGCTGTTGCACTTAGC+AGG 0.456577 2_1:+75830434 Msa0225680:CDS
GCTGCTTCTAATTACCCTCT+GGG 0.460105 2_1:+75829817 Msa0225680:CDS
AGTCGAACGCCGAGGAAAGA+AGG 0.460307 2_1:+75829743 Msa0225680:CDS
ATCAAATCACGGCGAAGACT+CGG 0.460731 2_1:-75829944 None:intergenic
AAGCTTCAAAACTGCAATGA+AGG 0.464293 2_1:+75830522 Msa0225680:CDS
AGAGGTTGTGGTTAGTGACT+TGG 0.467535 2_1:-75829074 None:intergenic
GGCAACCTGTGTTGTGGCTG+GGG 0.469028 2_1:-75832314 None:intergenic
CAAGCCCAAAGAAGAAGAGC+AGG 0.470250 2_1:+75830092 Msa0225680:CDS
CAAAATGGTGAACAGGGACT+TGG 0.473978 2_1:+75832965 Msa0225680:intron
AGGAATGTGTATTTCTAAAA+GGG 0.476882 2_1:+75829526 Msa0225680:five_prime_UTR
GTTAGAAATAAAGGAGGGAT+TGG 0.478873 2_1:+75830004 Msa0225680:CDS
AACGTTCCGGGACATGTAAT+AGG 0.479019 2_1:+75830284 Msa0225680:CDS
AAGGCAGTTGTTGCGAGAAT+CGG 0.482088 2_1:+75831769 Msa0225680:CDS
TTCAGCACTGTGAGAGGTTG+TGG 0.484899 2_1:-75829086 None:intergenic
AGGCAACCTGTGTTGTGGCT+GGG 0.485271 2_1:-75832315 None:intergenic
CGGGTGCGAGCCAAGTTGCT+TGG 0.485763 2_1:+75831498 Msa0225680:CDS
CCAAGTTGCTTGGTGGGTCA+TGG 0.487031 2_1:+75831508 Msa0225680:CDS
CCCCTTCCTATTACATGTCC+CGG 0.487747 2_1:-75830290 None:intergenic
CTGATAAAATGATTGAGAAA+AGG 0.490392 2_1:+75831750 Msa0225680:CDS
GAACAATGATATAGAAGAGC+AGG 0.492309 2_1:+75830168 Msa0225680:CDS
TGCGAGCCAAGTTGCTTGGT+GGG 0.493421 2_1:+75831502 Msa0225680:CDS
AGTAGCATCAACCCCGGAAA+GGG 0.494447 2_1:+75831842 Msa0225680:CDS
AATCTAAACAATGGCAAAAG+TGG 0.495011 2_1:+75831609 Msa0225680:CDS
GTGCGAGCCAAGTTGCTTGG+TGG 0.495804 2_1:+75831501 Msa0225680:CDS
ATCACTTTCCTTCTTTGCCA+AGG 0.496573 2_1:-75830044 None:intergenic
TACGCCGGTATTTCTTGGCC+CGG 0.496951 2_1:-75829912 None:intergenic
GAATCGGTCCGAAAAGAAAT+GGG 0.498856 2_1:+75831785 Msa0225680:CDS
TGTTTATGCCATAAACTCTC+AGG 0.500207 2_1:+75832357 Msa0225680:CDS
TCACAGAAGGAAGCAATTGT+TGG 0.502273 2_1:+75832150 Msa0225680:intron
TAGAACAATAAATGTAGCCT+TGG 0.503789 2_1:+75830027 Msa0225680:CDS
GAGTACTTCGACCGTTCAAA+GGG 0.504167 2_1:+75831542 Msa0225680:CDS
CCATACGTACTATTGGTTTG+AGG 0.507465 2_1:-75830202 None:intergenic
ATGGAGGAAAGCAGCAATAG+AGG 0.508277 2_1:+75829599 Msa0225680:CDS
CGGAGCTCCACTCTCAATGT+TGG 0.508341 2_1:+75830375 Msa0225680:CDS
CAGTTGGCAAGGAAGCAAAT+GGG 0.510086 2_1:+75833793 Msa0225680:CDS
AAACTATCTTCATTAAGATC+AGG 0.515217 2_1:+75829644 Msa0225680:CDS
CTTATCAATATGTGCCCACC+TGG 0.518074 2_1:-75829864 None:intergenic
TGAGAGTGGAGCTCCGGTCA+TGG 0.520701 2_1:-75830368 None:intergenic
GGAACCGGGCCAAGAAATAC+CGG 0.521618 2_1:+75829908 Msa0225680:CDS
GTGGGCACATATTGATAAGA+AGG 0.524950 2_1:+75829868 Msa0225680:CDS
AGAGTACTTCGACCGTTCAA+AGG 0.525221 2_1:+75831541 Msa0225680:CDS
GCTTTCTAGAAGACGAATCA+AGG 0.531246 2_1:+75830477 Msa0225680:CDS
GATGAACTGGCATTTGTAGC+CGG 0.532193 2_1:+75831692 Msa0225680:CDS
CCTCAAACCAATAGTACGTA+TGG 0.533615 2_1:+75830202 Msa0225680:CDS
AGATGGCAAAGTAGCTCTGA+AGG 0.536743 2_1:+75832015 Msa0225680:CDS
CTGATTCAAGAAGCAATAGC+TGG 0.538268 2_1:-75831865 None:intergenic
AACGAAGGAGAATTATTTCG+CGG 0.538604 2_1:-75829704 None:intergenic
AACGGTCGAAGTACTCTCTA+CGG 0.540457 2_1:-75831535 None:intergenic
AGCTGTTGCACTTAGCAGGA+AGG 0.547033 2_1:+75830438 Msa0225680:CDS
CAACATTGAGAGTGGAGCTC+CGG 0.548238 2_1:-75830374 None:intergenic
AGCAAATGGGGATAGAAGGC+AGG 0.552878 2_1:+75833806 Msa0225680:CDS
GTTCAGCTGTATGTGCATCA+TGG 0.555139 2_1:+75830563 Msa0225680:CDS
GCCAATTCCAACATTGAGAG+TGG 0.556981 2_1:-75830382 None:intergenic
GGAGCAATCGTGTGTTGTTG+TGG 0.563046 2_1:+75829795 Msa0225680:CDS
GCTGTTGCACTTAGCAGGAA+GGG 0.563411 2_1:+75830439 Msa0225680:CDS
TCCGGGACATGTAATAGGAA+GGG 0.564524 2_1:+75830289 Msa0225680:CDS
CTTGCACAGAGTAAAGATGT+TGG 0.568045 2_1:+75831568 Msa0225680:CDS
GAATCATGATTGTTGAGGAA+AGG 0.568364 2_1:+75829570 Msa0225680:exon
CCGGGACATGTAATAGGAAG+GGG 0.570061 2_1:+75830290 Msa0225680:CDS
TTCCGGGACATGTAATAGGA+AGG 0.571893 2_1:+75830288 Msa0225680:CDS
TAAATCTTCTCGACCAAACA+AGG 0.573246 2_1:-75830324 None:intergenic
GCAACTTGGCTCGCACCCGC+CGG 0.574124 2_1:-75831494 None:intergenic
AAGTAGCATCAACCCCGGAA+AGG 0.577074 2_1:+75831841 Msa0225680:CDS
GGTTCTCGGAACGGATTCTG+GGG 0.577256 2_1:+75833663 Msa0225680:intron
AATACAGAAGATTCCGTGCA+AGG 0.580479 2_1:-75833759 None:intergenic
ACTTGGAGAGACATTTGGAC+GGG 0.580802 2_1:+75832982 Msa0225680:CDS
GTTAAGTGTAGAAGGAATGC+CGG 0.582910 2_1:-75831711 None:intergenic
GGGCTTGCTTTACGACGAGA+AGG 0.583830 2_1:-75830076 None:intergenic
CTATCTTCATTAAGATCAGG+TGG 0.585023 2_1:+75829647 Msa0225680:CDS
CATGTCCCGGAACGTTTCTG+AGG 0.586620 2_1:-75830277 None:intergenic
CTCCGGTCATGGAAAGCTCG+TGG 0.587117 2_1:-75830357 None:intergenic
ATGGGAGCAGGTTCTCGGAA+CGG 0.587903 2_1:+75833654 Msa0225680:intron
TAAAGGAAGGCTTGTCTACA+CGG 0.590742 2_1:+75831951 Msa0225680:CDS
CTTGTCTGTTAATAATGAGA+CGG 0.594500 2_1:+75829982 Msa0225680:CDS
GAGACGGTTAGAAATAAAGG+AGG 0.595028 2_1:+75829998 Msa0225680:CDS
GCTGTATGTGCATCATGGAT+TGG 0.595255 2_1:+75830568 Msa0225680:CDS
ACTTTGCCATCTTTCAACAA+TGG 0.596363 2_1:-75832004 None:intergenic
TGGTAAGAAGAAACCAATTG+TGG 0.596946 2_1:+75830138 Msa0225680:CDS
AAGAGCAGGAGATCTTCGAA+AGG 0.599991 2_1:+75830106 Msa0225680:CDS
CCTCTGATAAAAGAAAATAG+AGG 0.600162 2_1:-75834024 None:intergenic
ATCTGCTGCAGAGTATAATG+AGG 0.601212 2_1:-75832335 None:intergenic
AAGTGCAACAGCTGAAACAG+TGG 0.603037 2_1:-75830428 None:intergenic
CTGAATCACAATCTAAACAA+TGG 0.603498 2_1:+75831600 Msa0225680:CDS
AAGACGAATCAAGGTGCTTG+AGG 0.605518 2_1:+75830486 Msa0225680:CDS
AAATTATCAAATATAAAGGA+AGG 0.606570 2_1:+75831938 Msa0225680:CDS
GCAATAGCTGGCCCTTTCCG+GGG 0.611467 2_1:-75831853 None:intergenic
CATGTATAAGAAATTTGTTG+AGG 0.611722 2_1:+75833861 Msa0225680:CDS
GTTGCACTTAGCAGGAAGGG+TGG 0.613077 2_1:+75830442 Msa0225680:CDS
GAGTTTAGTCTTCGAAACGA+AGG 0.614670 2_1:-75829719 None:intergenic
GACTTGGAGAGACATTTGGA+CGG 0.618285 2_1:+75832981 Msa0225680:CDS
AGTTCATCATTAACAGACAA+AGG 0.620196 2_1:-75831677 None:intergenic
ACGGGTGACTATAGAAGCAA+TGG 0.624266 2_1:+75833000 Msa0225680:CDS
AATACCAAGATAAGCCCAGA+GGG 0.624408 2_1:-75829831 None:intergenic
TGTGCAAGACACCCTTTGAA+CGG 0.624594 2_1:-75831553 None:intergenic
ATACATAATGTAGTGACTGC+TGG 0.625005 2_1:+75833967 Msa0225680:three_prime_UTR
AGTTGGCAAGGAAGCAAATG+GGG 0.626977 2_1:+75833794 Msa0225680:CDS
CGTCTCATTATTAACAGACA+AGG 0.627188 2_1:-75829981 None:intergenic
AAAGAAGGAAAGTGATGATG+AGG 0.629203 2_1:+75830051 Msa0225680:CDS
CAGGGAACGATGTCCTTGCA+CGG 0.630071 2_1:+75833746 Msa0225680:CDS
ATCCAATTGGACGTGCAGCA+GGG 0.637876 2_1:+75833728 Msa0225680:CDS
TGAGGAAAGGAATATAATGG+AGG 0.639776 2_1:+75829583 Msa0225680:CDS
GTGGACCCCAGCCACAACAC+AGG 0.642970 2_1:+75832309 Msa0225680:CDS
AGACGGTTAGAAATAAAGGA+GGG 0.643625 2_1:+75829999 Msa0225680:CDS
CATCACTAGCAATCAAATCA+CGG 0.645910 2_1:-75829955 None:intergenic
ACAAAGGAACAGTTGCAGGT+TGG 0.651753 2_1:-75831661 None:intergenic
TGTTGGATCCAAAAGTAACA+AGG 0.656667 2_1:+75832222 Msa0225680:CDS
GCTGTTCTAAGAAACTGAGA+AGG 0.659200 2_1:-75832261 None:intergenic
TAATGAGGCAACCTGTGTTG+TGG 0.663004 2_1:-75832320 None:intergenic
GGCACATATTGATAAGAAGG+AGG 0.663174 2_1:+75829871 Msa0225680:CDS
TCTTCGAAAGGTAAAGCTCG+TGG 0.669801 2_1:+75830118 Msa0225680:CDS
TCTCTAAGTAGCATCAACCC+CGG 0.669974 2_1:+75831836 Msa0225680:CDS
GTGAGAGTGAGAGAGAAACA+TGG 0.671462 2_1:-75829006 None:intergenic
GTTCTCGGAACGGATTCTGG+GGG 0.681613 2_1:+75833664 Msa0225680:CDS
AGTACGTATGGATTACTCGT+CGG 0.683292 2_1:+75830214 Msa0225680:CDS
ACTCGGATCAGCATCTACGC+CGG 0.684071 2_1:-75829927 None:intergenic
TGGATCCAAAAGTAACAAGG+TGG 0.684946 2_1:+75832225 Msa0225680:CDS
TAAACTCTAGTCGAACGCCG+AGG 0.690673 2_1:+75829735 Msa0225680:CDS
GTTGAGTTCAGCACTGTGAG+AGG 0.699896 2_1:-75829092 None:intergenic
CGGGACATGTAATAGGAAGG+GGG 0.712818 2_1:+75830291 Msa0225680:CDS
TATATGAATCATGATTGTTG+AGG 0.718092 2_1:+75829565 Msa0225680:exon
AAATACCAAGATAAGCCCAG+AGG 0.740468 2_1:-75829832 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAATAATTTAATATGCTT+AGG + chr2_1:75830855-75830874 Msa0225680:intron 10.0%
!! GAAAAAATTATCAAATATAA+AGG + chr2_1:75831934-75831953 Msa0225680:CDS 10.0%
!!! TTATTTTACTTTTTCTAATT+TGG + chr2_1:75832635-75832654 Msa0225680:intron 10.0%
!!! TTGTTTTTTTAATTACTTGA+AGG + chr2_1:75829413-75829432 Msa0225680:intron 15.0%
!!! TGTTTTTTTAATTACTTGAA+GGG + chr2_1:75829414-75829433 Msa0225680:intron 15.0%
!!! TATTTTAATATTTCCTTTTG+TGG - chr2_1:75830842-75830861 None:intergenic 15.0%
!! ATTTACAAGAGAAATATAAT+CGG - chr2_1:75831328-75831347 None:intergenic 15.0%
!! AAATTATCAAATATAAAGGA+AGG + chr2_1:75831938-75831957 Msa0225680:CDS 15.0%
!! GAAGTAAATTATATAACTTA+TGG + chr2_1:75832077-75832096 Msa0225680:intron 15.0%
!! TATTACATATATCAATATGA+AGG - chr2_1:75832433-75832452 None:intergenic 15.0%
!!! ATGTAATAATGCTTTTATAT+AGG + chr2_1:75832445-75832464 Msa0225680:intron 15.0%
!! AACAAAATCTATATATTTAG+AGG - chr2_1:75833454-75833473 None:intergenic 15.0%
!! AATATATTGCTTGATCAAAA+AGG - chr2_1:75829279-75829298 None:intergenic 20.0%
!!! ACTATTTTTTGGTGAATTTA+AGG + chr2_1:75829501-75829520 Msa0225680:five_prime_UTR 20.0%
!!! AGTTTTAAATCAACATTATC+AGG + chr2_1:75829671-75829690 Msa0225680:CDS 20.0%
!! TTGTTACATTTCATTATACA+TGG + chr2_1:75830602-75830621 Msa0225680:intron 20.0%
!! TTCAATGTTGCATATTAATT+TGG - chr2_1:75830754-75830773 None:intergenic 20.0%
!! TAATGGTTTGAATGATTAAA+AGG - chr2_1:75831079-75831098 None:intergenic 20.0%
!! TAATTCTTTCCTTTATTGTT+TGG + chr2_1:75831245-75831264 Msa0225680:intron 20.0%
!!! AATCATTTTATCAGTACTAA+AGG - chr2_1:75831744-75831763 None:intergenic 20.0%
!!! TCATTTAAAATATCTTTGCT+TGG - chr2_1:75832544-75832563 None:intergenic 20.0%
!! TGGGTAATTTATGAATTTAT+TGG + chr2_1:75832768-75832787 Msa0225680:intron 20.0%
!!! TTTATTGGAATAGTATTGAA+TGG + chr2_1:75832783-75832802 Msa0225680:intron 20.0%
!! AAAAAAGAATGATTATTGCA+GGG - chr2_1:75833917-75833936 None:intergenic 20.0%
!! AAAAAAAGAATGATTATTGC+AGG - chr2_1:75833918-75833937 None:intergenic 20.0%
! TATGCTAAAATCTCAAACTT+AGG - chr2_1:75829180-75829199 None:intergenic 25.0%
! TTTAGCAAATTAATCCATGT+GGG - chr2_1:75829391-75829410 None:intergenic 25.0%
!!! TTTTTGGTGAATTTAAGGAA+AGG + chr2_1:75829506-75829525 Msa0225680:five_prime_UTR 25.0%
!! AAGGAATGTGTATTTCTAAA+AGG + chr2_1:75829525-75829544 Msa0225680:five_prime_UTR 25.0%
!! AGGAATGTGTATTTCTAAAA+GGG + chr2_1:75829526-75829545 Msa0225680:five_prime_UTR 25.0%
! TATATGAATCATGATTGTTG+AGG + chr2_1:75829565-75829584 Msa0225680:exon 25.0%
! AAACTATCTTCATTAAGATC+AGG + chr2_1:75829644-75829663 Msa0225680:CDS 25.0%
! TTGTTCTTAATTTCCACAAT+TGG - chr2_1:75830154-75830173 None:intergenic 25.0%
! GTTTGAATGATTAAAAGGTT+AGG - chr2_1:75831074-75831093 None:intergenic 25.0%
! TTAAACTCATTCGTTTCTAA+TGG - chr2_1:75831096-75831115 None:intergenic 25.0%
! TAGAACTAGAAAATGGTATA+AGG - chr2_1:75831157-75831176 None:intergenic 25.0%
! TAAAGGTTAGAACTAGAAAA+TGG - chr2_1:75831164-75831183 None:intergenic 25.0%
! TCTAACCTTTATATATATCG+TGG + chr2_1:75831173-75831192 Msa0225680:intron 25.0%
! ATCTACCACGATATATATAA+AGG - chr2_1:75831181-75831200 None:intergenic 25.0%
! TTTATTAAGACATGTAGAAG+AGG - chr2_1:75831427-75831446 None:intergenic 25.0%
! AAACGTTCAATATATTGTTC+TGG - chr2_1:75831473-75831492 None:intergenic 25.0%
!! ACAATATATTGAACGTTTTC+CGG + chr2_1:75831475-75831494 Msa0225680:CDS 25.0%
!! ATTTTATCAGTACTAAAGGA+CGG - chr2_1:75831740-75831759 None:intergenic 25.0%
! CTGATAAAATGATTGAGAAA+AGG + chr2_1:75831750-75831769 Msa0225680:CDS 25.0%
!!! AAAACTGATTTTGAAGTGTT+TGG - chr2_1:75832289-75832308 None:intergenic 25.0%
!! GTATTCTAAAAAAAGAACCA+AGG - chr2_1:75832698-75832717 None:intergenic 25.0%
! TCAGTATTCTTTATCAGTAA+AGG + chr2_1:75832734-75832753 Msa0225680:intron 25.0%
! AGTAAAGGATAAGATTTACT+GGG + chr2_1:75832749-75832768 Msa0225680:intron 25.0%
!!! ATAGTATTGAATGGAAGTTT+TGG + chr2_1:75832792-75832811 Msa0225680:intron 25.0%
!!! ATAGTAATAGCTCTAAATGT+AGG - chr2_1:75832901-75832920 None:intergenic 25.0%
! ACAATAATCAAAAGGATCAA+AGG - chr2_1:75833354-75833373 None:intergenic 25.0%
! CTTCAGTAACAATAATCAAA+AGG - chr2_1:75833362-75833381 None:intergenic 25.0%
!!! TTGTTCTAAAACTAGGAAAA+AGG + chr2_1:75833494-75833513 Msa0225680:intron 25.0%
! TTGTGTTCCAATTAATCTTA+TGG + chr2_1:75833635-75833654 Msa0225680:intron 25.0%
! TGTGTTCCAATTAATCTTAT+GGG + chr2_1:75833636-75833655 Msa0225680:intron 25.0%
!!! CATGTATAAGAAATTTGTTG+AGG + chr2_1:75833861-75833880 Msa0225680:CDS 25.0%
!!! AATTTGTTGAGGTTATTGTT+AGG + chr2_1:75833872-75833891 Msa0225680:CDS 25.0%
! TTGCATGATCTTTTACTACT+TGG + chr2_1:75829233-75829252 Msa0225680:intron 30.0%
CTTTAGCAAATTAATCCATG+TGG - chr2_1:75829392-75829411 None:intergenic 30.0%
!!! AGCATTGTAGGACTATTTTT+TGG + chr2_1:75829490-75829509 Msa0225680:five_prime_UTR 30.0%
TGTTGAGGAAAGGAATATAA+TGG + chr2_1:75829580-75829599 Msa0225680:CDS 30.0%
!!! TTGGTATTTTTTTTCAGTCC+AGG + chr2_1:75829846-75829865 Msa0225680:CDS 30.0%
!!! TATTTTTTTTCAGTCCAGGT+GGG + chr2_1:75829850-75829869 Msa0225680:CDS 30.0%
CTTGTCTGTTAATAATGAGA+CGG + chr2_1:75829982-75830001 Msa0225680:CDS 30.0%
AATGAGACGGTTAGAAATAA+AGG + chr2_1:75829995-75830014 Msa0225680:CDS 30.0%
TAGAACAATAAATGTAGCCT+TGG + chr2_1:75830027-75830046 Msa0225680:CDS 30.0%
TAAGATACAAAAGTAGAGGA+AGG - chr2_1:75830699-75830718 None:intergenic 30.0%
! AGTCTAAGATACAAAAGTAG+AGG - chr2_1:75830703-75830722 None:intergenic 30.0%
!!! TAAATGCTTTTTCAACCTCA+CGG + chr2_1:75830726-75830745 Msa0225680:intron 30.0%
AACTAACAAGCATATCTTTC+TGG - chr2_1:75830796-75830815 None:intergenic 30.0%
! ATAGAGCTAGTATGATAATG+TGG - chr2_1:75830930-75830949 None:intergenic 30.0%
!! CATACTAGCTCTATAACTAT+TGG + chr2_1:75830936-75830955 Msa0225680:intron 30.0%
!! AGCTCTATAACTATTGGTTT+AGG + chr2_1:75830942-75830961 Msa0225680:intron 30.0%
GACACAATACCAAACAATAA+AGG - chr2_1:75831257-75831276 None:intergenic 30.0%
! TGATTTTGCTTCTAATACAG+AGG + chr2_1:75831298-75831317 Msa0225680:intron 30.0%
CTGAATCACAATCTAAACAA+TGG + chr2_1:75831600-75831619 Msa0225680:CDS 30.0%
AATCTAAACAATGGCAAAAG+TGG + chr2_1:75831609-75831628 Msa0225680:CDS 30.0%
AGTTCATCATTAACAGACAA+AGG - chr2_1:75831680-75831699 None:intergenic 30.0%
TTTGTCTGTTAATGATGAAC+TGG + chr2_1:75831679-75831698 Msa0225680:CDS 30.0%
! CAGTTAATCCCATTTCTTTT+CGG - chr2_1:75831796-75831815 None:intergenic 30.0%
TTGTTACCATTGTTGAAAGA+TGG + chr2_1:75831998-75832017 Msa0225680:CDS 30.0%
TTAAGCAGGTTACATTAAAC+TGG + chr2_1:75832055-75832074 Msa0225680:intron 30.0%
!!! ATAATCCACCTTGTTACTTT+TGG - chr2_1:75832233-75832252 None:intergenic 30.0%
! ACACTTCAAAATCAGTTTTG+TGG + chr2_1:75832290-75832309 Msa0225680:CDS 30.0%
GGATGCATATACTCATAATT+AGG - chr2_1:75832484-75832503 None:intergenic 30.0%
! AAGACTTGAAGTTTTTACCT+TGG + chr2_1:75832678-75832697 Msa0225680:intron 30.0%
TACTGAATATACAGACAAGA+AGG - chr2_1:75832720-75832739 None:intergenic 30.0%
CAGTAAAGGATAAGATTTAC+TGG + chr2_1:75832748-75832767 Msa0225680:intron 30.0%
TACGAAAATATCCTCCAAAA+TGG + chr2_1:75832924-75832943 Msa0225680:intron 30.0%
ACGAAAATATCCTCCAAAAT+GGG + chr2_1:75832925-75832944 Msa0225680:intron 30.0%
ACTGTTGCTAACTAACAAAA+TGG + chr2_1:75832950-75832969 Msa0225680:intron 30.0%
TAACTAACAAAATGGTGAAC+AGG + chr2_1:75832958-75832977 Msa0225680:intron 30.0%
AACTAACAAAATGGTGAACA+GGG + chr2_1:75832959-75832978 Msa0225680:intron 30.0%
ATTTGCATGAGATAAACAAG+AGG - chr2_1:75833061-75833080 None:intergenic 30.0%
!! TTTTATTTGAGTGTCACACT+TGG + chr2_1:75833086-75833105 Msa0225680:intron 30.0%
TTTATTTGAGTGTCACACTT+GGG + chr2_1:75833087-75833106 Msa0225680:intron 30.0%
! ACTGTTTTCGTAAGCTATAT+TGG + chr2_1:75833205-75833224 Msa0225680:intron 30.0%
! TTGTGGTAGTAAGTTGAAAA+CGG + chr2_1:75833233-75833252 Msa0225680:intron 30.0%
!! TTGTCAGTTGTTCTAAAACT+AGG + chr2_1:75833487-75833506 Msa0225680:intron 30.0%
TAATTTAGTGCCATAAACCT+AGG - chr2_1:75833522-75833541 None:intergenic 30.0%
TCACTCATTCATTTACATTC+AGG - chr2_1:75833609-75833628 None:intergenic 30.0%
ATAGTTGAGAACAATGTTAC+AGG + chr2_1:75833697-75833716 Msa0225680:CDS 30.0%
GTATTTGCTTAAAAACCAGT+TGG + chr2_1:75833777-75833796 Msa0225680:CDS 30.0%
CCTCTGATAAAAGAAAATAG+AGG - chr2_1:75834027-75834046 None:intergenic 30.0%
! CCTCTATTTTCTTTTATCAG+AGG + chr2_1:75834024-75834043 Msa0225680:three_prime_UTR 30.0%
GGGAAAAACTATTGATACTT+TGG - chr2_1:75834131-75834150 None:intergenic 30.0%
TGTTATAAAGATAGCCCACA+TGG + chr2_1:75829374-75829393 Msa0225680:intron 35.0%
!! GTTCAAGATTGAAGCATTGT+AGG + chr2_1:75829478-75829497 Msa0225680:five_prime_UTR 35.0%
GAATCATGATTGTTGAGGAA+AGG + chr2_1:75829570-75829589 Msa0225680:exon 35.0%
TGAGGAAAGGAATATAATGG+AGG + chr2_1:75829583-75829602 Msa0225680:CDS 35.0%
CTATCTTCATTAAGATCAGG+TGG + chr2_1:75829647-75829666 Msa0225680:CDS 35.0%
AACGAAGGAGAATTATTTCG+CGG - chr2_1:75829707-75829726 None:intergenic 35.0%
AAAGTTCTTCCTTCTTTCCT+CGG - chr2_1:75829755-75829774 None:intergenic 35.0%
! AGGAAAGAAGGAAGAACTTT+TGG + chr2_1:75829755-75829774 Msa0225680:CDS 35.0%
! AAAGAAGGAAGAACTTTTGG+TGG + chr2_1:75829758-75829777 Msa0225680:CDS 35.0%
!! TTGGTGGTTTTCTGTTGTTT+CGG + chr2_1:75829774-75829793 Msa0225680:CDS 35.0%
!!! GTATTTTTTTTCAGTCCAGG+TGG + chr2_1:75829849-75829868 Msa0225680:CDS 35.0%
! GATAAGAAGGAGGAATTTTC+TGG + chr2_1:75829881-75829900 Msa0225680:CDS 35.0%
CATCACTAGCAATCAAATCA+CGG - chr2_1:75829958-75829977 None:intergenic 35.0%
CGTCTCATTATTAACAGACA+AGG - chr2_1:75829984-75830003 None:intergenic 35.0%
AGACGGTTAGAAATAAAGGA+GGG + chr2_1:75829999-75830018 Msa0225680:CDS 35.0%
GTTAGAAATAAAGGAGGGAT+TGG + chr2_1:75830004-75830023 Msa0225680:CDS 35.0%
! AAAGAAGGAAAGTGATGATG+AGG + chr2_1:75830051-75830070 Msa0225680:CDS 35.0%
TGGTAAGAAGAAACCAATTG+TGG + chr2_1:75830138-75830157 Msa0225680:CDS 35.0%
GAACAATGATATAGAAGAGC+AGG + chr2_1:75830168-75830187 Msa0225680:CDS 35.0%
!! CTATTGGTTTGAGGAATTTC+TGG - chr2_1:75830196-75830215 None:intergenic 35.0%
GAGTAATCCATACGTACTAT+TGG - chr2_1:75830212-75830231 None:intergenic 35.0%
! CTCAACAGAAGATAGGATTT+TGG + chr2_1:75830246-75830265 Msa0225680:CDS 35.0%
!! CAGAAGATAGGATTTTGGAA+TGG + chr2_1:75830251-75830270 Msa0225680:CDS 35.0%
TAAATCTTCTCGACCAAACA+AGG - chr2_1:75830327-75830346 None:intergenic 35.0%
AAGCTTCAAAACTGCAATGA+AGG + chr2_1:75830522-75830541 Msa0225680:CDS 35.0%
AAAGTAGAGGAAGGAAAAGA+TGG - chr2_1:75830690-75830709 None:intergenic 35.0%
GATATTGTAGCTACCACAAA+AGG + chr2_1:75830826-75830845 Msa0225680:intron 35.0%
! TTTTGCAATGTTGCAATGTC+CGG - chr2_1:75830898-75830917 None:intergenic 35.0%
!!! GACACTTGAGTTTTGAAGTA+TGG - chr2_1:75830988-75831007 None:intergenic 35.0%
AACTCAAGTGTCTCAGTAAA+TGG + chr2_1:75830996-75831015 Msa0225680:intron 35.0%
ATTGTTTGGTATTGTGTCCA+TGG + chr2_1:75831259-75831278 Msa0225680:intron 35.0%
AGTAACAGCTAAACAATCCA+TGG - chr2_1:75831279-75831298 None:intergenic 35.0%
CTGGAGACATAAGAAAACAA+AGG - chr2_1:75831454-75831473 None:intergenic 35.0%
! ATATATTGAACGTTTTCCGG+CGG + chr2_1:75831478-75831497 Msa0225680:CDS 35.0%
ACTTTGCCATCTTTCAACAA+TGG - chr2_1:75832007-75832026 None:intergenic 35.0%
CCTGCTTAAAGAATTGTTTG+AGG - chr2_1:75832044-75832063 None:intergenic 35.0%
CCTCAAACAATTCTTTAAGC+AGG + chr2_1:75832041-75832060 Msa0225680:CDS 35.0%
TGAACTTTGACAATCACAGA+AGG + chr2_1:75832137-75832156 Msa0225680:intron 35.0%
CAATCTCTCAAAGTTCTCAT+TGG + chr2_1:75832196-75832215 Msa0225680:CDS 35.0%
!! AAAGTTCTCATTGGTTCTGT+TGG + chr2_1:75832205-75832224 Msa0225680:CDS 35.0%
TGTTGGATCCAAAAGTAACA+AGG + chr2_1:75832222-75832241 Msa0225680:CDS 35.0%
ACGAAACTGTTCAATCATGT+TGG + chr2_1:75832563-75832582 Msa0225680:intron 35.0%
! TTAGCAACAGTTTCCCATTT+TGG - chr2_1:75832941-75832960 None:intergenic 35.0%
ACTATAGAAGCAATGGCATT+TGG + chr2_1:75833007-75833026 Msa0225680:CDS 35.0%
AAGCTATATTGGAAAGCTTG+TGG + chr2_1:75833216-75833235 Msa0225680:intron 35.0%
CTACTAACATTCGAACTTGA+GGG - chr2_1:75833302-75833321 None:intergenic 35.0%
TCTACTAACATTCGAACTTG+AGG - chr2_1:75833303-75833322 None:intergenic 35.0%
TCAAATGATCCAATCCAAAC+AGG + chr2_1:75833328-75833347 Msa0225680:intron 35.0%
! TGTGCCTTTCTTTCAGTTTT+AGG - chr2_1:75833411-75833430 None:intergenic 35.0%
TATTTAGAGGCAACCACTAT+CGG - chr2_1:75833441-75833460 None:intergenic 35.0%
ATACATAATGTAGTGACTGC+TGG + chr2_1:75833967-75833986 Msa0225680:three_prime_UTR 35.0%
CAGCAATAGAGGTGAATTTC+AGG + chr2_1:75829610-75829629 Msa0225680:CDS 40.0%
! GAGTTTAGTCTTCGAAACGA+AGG - chr2_1:75829722-75829741 None:intergenic 40.0%
AATACCAAGATAAGCCCAGA+GGG - chr2_1:75829834-75829853 None:intergenic 40.0%
AAATACCAAGATAAGCCCAG+AGG - chr2_1:75829835-75829854 None:intergenic 40.0%
GTGGGCACATATTGATAAGA+AGG + chr2_1:75829868-75829887 Msa0225680:CDS 40.0%
GGCACATATTGATAAGAAGG+AGG + chr2_1:75829871-75829890 Msa0225680:CDS 40.0%
!!! AAGGAGGAATTTTCTGGCTT+TGG + chr2_1:75829887-75829906 Msa0225680:CDS 40.0%
!!! GAATTTTCTGGCTTTGGAAC+CGG + chr2_1:75829893-75829912 Msa0225680:CDS 40.0%
!!! AATTTTCTGGCTTTGGAACC+GGG + chr2_1:75829894-75829913 Msa0225680:CDS 40.0%
GAGACGGTTAGAAATAAAGG+AGG + chr2_1:75829998-75830017 Msa0225680:CDS 40.0%
! AAATGTAGCCTTGGCAAAGA+AGG + chr2_1:75830036-75830055 Msa0225680:CDS 40.0%
ATCACTTTCCTTCTTTGCCA+AGG - chr2_1:75830047-75830066 None:intergenic 40.0%
ATCTCCTGCTCTTCTTCTTT+GGG - chr2_1:75830099-75830118 None:intergenic 40.0%
!! CCATACGTACTATTGGTTTG+AGG - chr2_1:75830205-75830224 None:intergenic 40.0%
CCTCAAACCAATAGTACGTA+TGG + chr2_1:75830202-75830221 Msa0225680:CDS 40.0%
! AGTACGTATGGATTACTCGT+CGG + chr2_1:75830214-75830233 Msa0225680:CDS 40.0%
CTATCTTCTGTTGAGCCAAA+TGG - chr2_1:75830241-75830260 None:intergenic 40.0%
! CATTTGGCTCAACAGAAGAT+AGG + chr2_1:75830239-75830258 Msa0225680:CDS 40.0%
TCCACTCTCAATGTTGGAAT+TGG + chr2_1:75830381-75830400 Msa0225680:CDS 40.0%
! GCTTTCTAGAAGACGAATCA+AGG + chr2_1:75830477-75830496 Msa0225680:CDS 40.0%
AAGGCTGATCTTGTAATTGC+TGG + chr2_1:75830541-75830560 Msa0225680:CDS 40.0%
! CATATTAATTTGGCACCGTG+AGG - chr2_1:75830744-75830763 None:intergenic 40.0%
GGACATTGCAACATTGCAAA+AGG + chr2_1:75830897-75830916 Msa0225680:intron 40.0%
! TGAAGTATGGCATGCTTTTG+TGG - chr2_1:75830975-75830994 None:intergenic 40.0%
TCAGTAAATGGCACCAACTT+TGG + chr2_1:75831008-75831027 Msa0225680:intron 40.0%
GTATGTTGAGGATCCAAAGT+TGG - chr2_1:75831024-75831043 None:intergenic 40.0%
GCAGGAATATAGGTATGTTG+AGG - chr2_1:75831036-75831055 None:intergenic 40.0%
GTGCATATGTGCAGGAATAT+AGG - chr2_1:75831046-75831065 None:intergenic 40.0%
! TATATTGAACGTTTTCCGGC+GGG + chr2_1:75831479-75831498 Msa0225680:CDS 40.0%
CTTGCACAGAGTAAAGATGT+TGG + chr2_1:75831568-75831587 Msa0225680:CDS 40.0%
GTTAAGTGTAGAAGGAATGC+CGG - chr2_1:75831714-75831733 None:intergenic 40.0%
AGAATCGGTCCGAAAAGAAA+TGG + chr2_1:75831784-75831803 Msa0225680:CDS 40.0%
GAATCGGTCCGAAAAGAAAT+GGG + chr2_1:75831785-75831804 Msa0225680:CDS 40.0%
CTGATTCAAGAAGCAATAGC+TGG - chr2_1:75831868-75831887 None:intergenic 40.0%
TAAAGGAAGGCTTGTCTACA+CGG + chr2_1:75831951-75831970 Msa0225680:CDS 40.0%
TCACAGAAGGAAGCAATTGT+TGG + chr2_1:75832150-75832169 Msa0225680:intron 40.0%
TGGATCCAAAAGTAACAAGG+TGG + chr2_1:75832225-75832244 Msa0225680:CDS 40.0%
!! GCTGTTCTAAGAAACTGAGA+AGG - chr2_1:75832264-75832283 None:intergenic 40.0%
ATCTGCTGCAGAGTATAATG+AGG - chr2_1:75832338-75832357 None:intergenic 40.0%
GGGCAAAGTAGATTCATAAG+TGG - chr2_1:75832505-75832524 None:intergenic 40.0%
TTGGAGCTATGTGAATGTCA+GGG - chr2_1:75832525-75832544 None:intergenic 40.0%
CTCTAAATGTAGGTAACAGC+TGG - chr2_1:75832891-75832910 None:intergenic 40.0%
AAGCAAACTAGCAGTCATAC+CGG - chr2_1:75833037-75833056 None:intergenic 40.0%
AAAAGGATCAAAGGCCTGTT+TGG - chr2_1:75833345-75833364 None:intergenic 40.0%
!!! CCTTTCTTTCAGTTTTAGGC+TGG - chr2_1:75833407-75833426 None:intergenic 40.0%
CCAGCCTAAAACTGAAAGAA+AGG + chr2_1:75833404-75833423 Msa0225680:intron 40.0%
AAACTAGGAAAAAGGCTCCT+AGG + chr2_1:75833502-75833521 Msa0225680:intron 40.0%
GAAAAAGGCTCCTAGGTTTA+TGG + chr2_1:75833509-75833528 Msa0225680:intron 40.0%
CCTGCTCCCATAAGATTAAT+TGG - chr2_1:75833645-75833664 None:intergenic 40.0%
CCAATTAATCTTATGGGAGC+AGG + chr2_1:75833642-75833661 Msa0225680:intron 40.0%
ACAGGTCTTCTTCATCCAAT+TGG + chr2_1:75833715-75833734 Msa0225680:CDS 40.0%
AATACAGAAGATTCCGTGCA+AGG - chr2_1:75833762-75833781 None:intergenic 40.0%
TGCTTAAAAACCAGTTGGCA+AGG + chr2_1:75833782-75833801 Msa0225680:CDS 40.0%
AGAGCTAGCTAGTCACAAAT+TGG - chr2_1:75834004-75834023 None:intergenic 40.0%
GTGAGAGTGAGAGAGAAACA+TGG - chr2_1:75829009-75829028 None:intergenic 45.0%
AGAGGTTGTGGTTAGTGACT+TGG - chr2_1:75829077-75829096 None:intergenic 45.0%
ATGGAGGAAAGCAGCAATAG+AGG + chr2_1:75829599-75829618 Msa0225680:CDS 45.0%
GCTGCTTCTAATTACCCTCT+GGG + chr2_1:75829817-75829836 Msa0225680:CDS 45.0%
ATTACCCTCTGGGCTTATCT+TGG + chr2_1:75829827-75829846 Msa0225680:CDS 45.0%
CTTATCAATATGTGCCCACC+TGG - chr2_1:75829867-75829886 None:intergenic 45.0%
ATCAAATCACGGCGAAGACT+CGG - chr2_1:75829947-75829966 None:intergenic 45.0%
GATCTCCTGCTCTTCTTCTT+TGG - chr2_1:75830100-75830119 None:intergenic 45.0%
AAGAGCAGGAGATCTTCGAA+AGG + chr2_1:75830106-75830125 Msa0225680:CDS 45.0%
TCTTCGAAAGGTAAAGCTCG+TGG + chr2_1:75830118-75830137 Msa0225680:CDS 45.0%
AACGTTCCGGGACATGTAAT+AGG + chr2_1:75830284-75830303 Msa0225680:CDS 45.0%
TTCCGGGACATGTAATAGGA+AGG + chr2_1:75830288-75830307 Msa0225680:CDS 45.0%
TCCGGGACATGTAATAGGAA+GGG + chr2_1:75830289-75830308 Msa0225680:CDS 45.0%
GCCAATTCCAACATTGAGAG+TGG - chr2_1:75830385-75830404 None:intergenic 45.0%
AAGTGCAACAGCTGAAACAG+TGG - chr2_1:75830431-75830450 None:intergenic 45.0%
TTTCAGCTGTTGCACTTAGC+AGG + chr2_1:75830434-75830453 Msa0225680:CDS 45.0%
! AAGACGAATCAAGGTGCTTG+AGG + chr2_1:75830486-75830505 Msa0225680:CDS 45.0%
GTTCAGCTGTATGTGCATCA+TGG + chr2_1:75830563-75830582 Msa0225680:CDS 45.0%
GCTGTATGTGCATCATGGAT+TGG + chr2_1:75830568-75830587 Msa0225680:CDS 45.0%
CAATGTCCGGTCAAAAGACT+AGG - chr2_1:75830885-75830904 None:intergenic 45.0%
AGGAGTTTGTGCATATGTGC+AGG - chr2_1:75831054-75831073 None:intergenic 45.0%
! AACGGTCGAAGTACTCTCTA+CGG - chr2_1:75831538-75831557 None:intergenic 45.0%
! AGAGTACTTCGACCGTTCAA+AGG + chr2_1:75831541-75831560 Msa0225680:CDS 45.0%
GAGTACTTCGACCGTTCAAA+GGG + chr2_1:75831542-75831561 Msa0225680:CDS 45.0%
TGTGCAAGACACCCTTTGAA+CGG - chr2_1:75831556-75831575 None:intergenic 45.0%
ACAAAGGAACAGTTGCAGGT+TGG - chr2_1:75831664-75831683 None:intergenic 45.0%
ACAGACAAAGGAACAGTTGC+AGG - chr2_1:75831668-75831687 None:intergenic 45.0%
GATGAACTGGCATTTGTAGC+CGG + chr2_1:75831692-75831711 Msa0225680:CDS 45.0%
! GACGGAGTGTTAAGTGTAGA+AGG - chr2_1:75831722-75831741 None:intergenic 45.0%
AAGGCAGTTGTTGCGAGAAT+CGG + chr2_1:75831769-75831788 Msa0225680:CDS 45.0%
TCTCTAAGTAGCATCAACCC+CGG + chr2_1:75831836-75831855 Msa0225680:CDS 45.0%
!! AGATGGCAAAGTAGCTCTGA+AGG + chr2_1:75832015-75832034 Msa0225680:CDS 45.0%
TAATGAGGCAACCTGTGTTG+TGG - chr2_1:75832323-75832342 None:intergenic 45.0%
CTTGGAGCTATGTGAATGTC+AGG - chr2_1:75832526-75832545 None:intergenic 45.0%
! GCAACAGTTTCCCATTTTGG+AGG - chr2_1:75832938-75832957 None:intergenic 45.0%
CAAAATGGTGAACAGGGACT+TGG + chr2_1:75832965-75832984 Msa0225680:intron 45.0%
GACTTGGAGAGACATTTGGA+CGG + chr2_1:75832981-75833000 Msa0225680:CDS 45.0%
ACTTGGAGAGACATTTGGAC+GGG + chr2_1:75832982-75833001 Msa0225680:CDS 45.0%
ACGGGTGACTATAGAAGCAA+TGG + chr2_1:75833000-75833019 Msa0225680:CDS 45.0%
! AGCAATGGCATTTGGTCTTC+CGG + chr2_1:75833015-75833034 Msa0225680:CDS 45.0%
TTCGAACTTGAGGGACTGTT+TGG - chr2_1:75833293-75833312 None:intergenic 45.0%
GATCAAAGGCCTGTTTGGAT+TGG - chr2_1:75833340-75833359 None:intergenic 45.0%
ATCTTATGGGAGCAGGTTCT+CGG + chr2_1:75833649-75833668 Msa0225680:intron 45.0%
CAGTTGGCAAGGAAGCAAAT+GGG + chr2_1:75833793-75833812 Msa0225680:CDS 45.0%
AGTTGGCAAGGAAGCAAATG+GGG + chr2_1:75833794-75833813 Msa0225680:CDS 45.0%
AGGAAGCAAATGGGGATAGA+AGG + chr2_1:75833802-75833821 Msa0225680:CDS 45.0%
GATAGAAGGCAGGAAAAAGG+TGG + chr2_1:75833816-75833835 Msa0225680:CDS 45.0%
!!! AAATATTTTTTAATTTTCAA+TGG - chr2_1:75832827-75832846 None:intergenic 5.0%
TTCAGCACTGTGAGAGGTTG+TGG - chr2_1:75829089-75829108 None:intergenic 50.0%
GTTGAGTTCAGCACTGTGAG+AGG - chr2_1:75829095-75829114 None:intergenic 50.0%
TAAACTCTAGTCGAACGCCG+AGG + chr2_1:75829735-75829754 Msa0225680:CDS 50.0%
! GGAGCAATCGTGTGTTGTTG+TGG + chr2_1:75829795-75829814 Msa0225680:CDS 50.0%
GGCTGCTTCTAATTACCCTC+TGG + chr2_1:75829816-75829835 Msa0225680:CDS 50.0%
GCATCTACGCCGGTATTTCT+TGG - chr2_1:75829920-75829939 None:intergenic 50.0%
CAAGCCCAAAGAAGAAGAGC+AGG + chr2_1:75830092-75830111 Msa0225680:CDS 50.0%
GGATTACTCGTCGGTCCATT+TGG + chr2_1:75830223-75830242 Msa0225680:CDS 50.0%
TGGAGTCCTCAGAAACGTTC+CGG + chr2_1:75830271-75830290 Msa0225680:CDS 50.0%
CCCCTTCCTATTACATGTCC+CGG - chr2_1:75830293-75830312 None:intergenic 50.0%
CCGGGACATGTAATAGGAAG+GGG + chr2_1:75830290-75830309 Msa0225680:CDS 50.0%
CGGGACATGTAATAGGAAGG+GGG + chr2_1:75830291-75830310 Msa0225680:CDS 50.0%
TTCCACGAGCTTTCCATGAC+CGG + chr2_1:75830355-75830374 Msa0225680:CDS 50.0%
CAACATTGAGAGTGGAGCTC+CGG - chr2_1:75830377-75830396 None:intergenic 50.0%
! GCAACAGAGCTTTTGAGCTG+TGG + chr2_1:75830403-75830422 Msa0225680:CDS 50.0%
AGCTGTTGCACTTAGCAGGA+AGG + chr2_1:75830438-75830457 Msa0225680:CDS 50.0%
GCTGTTGCACTTAGCAGGAA+GGG + chr2_1:75830439-75830458 Msa0225680:CDS 50.0%
!! GGACGACCTAGTCTTTTGAC+CGG + chr2_1:75830876-75830895 Msa0225680:intron 50.0%
AAGTAGCATCAACCCCGGAA+AGG + chr2_1:75831841-75831860 Msa0225680:CDS 50.0%
AGTAGCATCAACCCCGGAAA+GGG + chr2_1:75831842-75831861 Msa0225680:CDS 50.0%
AAGCAATAGCTGGCCCTTTC+CGG - chr2_1:75831858-75831877 None:intergenic 50.0%
CAGGGACTTGGAGAGACATT+TGG + chr2_1:75832977-75832996 Msa0225680:intron 50.0%
TAGAGGCAACCACTATCGGA+AGG - chr2_1:75833437-75833456 None:intergenic 50.0%
! AGGTTCTCGGAACGGATTCT+GGG + chr2_1:75833662-75833681 Msa0225680:intron 50.0%
ATCCAATTGGACGTGCAGCA+GGG + chr2_1:75833728-75833747 Msa0225680:CDS 50.0%
CCATTTGCTTCCTTGCCAAC+TGG - chr2_1:75833795-75833814 None:intergenic 50.0%
CCAGTTGGCAAGGAAGCAAA+TGG + chr2_1:75833792-75833811 Msa0225680:CDS 50.0%
AGCAAATGGGGATAGAAGGC+AGG + chr2_1:75833806-75833825 Msa0225680:CDS 50.0%
GGGGATAGAAGGCAGGAAAA+AGG + chr2_1:75833813-75833832 Msa0225680:CDS 50.0%
AGTCGAACGCCGAGGAAAGA+AGG + chr2_1:75829743-75829762 Msa0225680:CDS 55.0%
GGAACCGGGCCAAGAAATAC+CGG + chr2_1:75829908-75829927 Msa0225680:CDS 55.0%
TACGCCGGTATTTCTTGGCC+CGG - chr2_1:75829915-75829934 None:intergenic 55.0%
ACTCGGATCAGCATCTACGC+CGG - chr2_1:75829930-75829949 None:intergenic 55.0%
! GGGCTTGCTTTACGACGAGA+AGG - chr2_1:75830079-75830098 None:intergenic 55.0%
GGAGTCCTCAGAAACGTTCC+GGG + chr2_1:75830272-75830291 Msa0225680:CDS 55.0%
CATGTCCCGGAACGTTTCTG+AGG - chr2_1:75830280-75830299 None:intergenic 55.0%
! GGGACTTTGCACGCCTTGTT+TGG + chr2_1:75830311-75830330 Msa0225680:CDS 55.0%
CGGAGCTCCACTCTCAATGT+TGG + chr2_1:75830375-75830394 Msa0225680:CDS 55.0%
GTTGCACTTAGCAGGAAGGG+TGG + chr2_1:75830442-75830461 Msa0225680:CDS 55.0%
TGCGAGCCAAGTTGCTTGGT+GGG + chr2_1:75831502-75831521 Msa0225680:CDS 55.0%
CCATGACCCACCAAGCAACT+TGG - chr2_1:75831511-75831530 None:intergenic 55.0%
! CCAAGTTGCTTGGTGGGTCA+TGG + chr2_1:75831508-75831527 Msa0225680:CDS 55.0%
AGCAATAGCTGGCCCTTTCC+GGG - chr2_1:75831857-75831876 None:intergenic 55.0%
AGGCAACCTGTGTTGTGGCT+GGG - chr2_1:75832318-75832337 None:intergenic 55.0%
ATGGGAGCAGGTTCTCGGAA+CGG + chr2_1:75833654-75833673 Msa0225680:intron 55.0%
! CAGGTTCTCGGAACGGATTC+TGG + chr2_1:75833661-75833680 Msa0225680:intron 55.0%
! GGTTCTCGGAACGGATTCTG+GGG + chr2_1:75833663-75833682 Msa0225680:intron 55.0%
! GTTCTCGGAACGGATTCTGG+GGG + chr2_1:75833664-75833683 Msa0225680:CDS 55.0%
! AACGGATTCTGGGGGAACGA+AGG + chr2_1:75833672-75833691 Msa0225680:CDS 55.0%
CATCCAATTGGACGTGCAGC+AGG + chr2_1:75833727-75833746 Msa0225680:CDS 55.0%
TTCCCTGCTGCACGTCCAAT+TGG - chr2_1:75833733-75833752 None:intergenic 55.0%
CAGGGAACGATGTCCTTGCA+CGG + chr2_1:75833746-75833765 Msa0225680:CDS 55.0%
CTCCGGTCATGGAAAGCTCG+TGG - chr2_1:75830360-75830379 None:intergenic 60.0%
TGAGAGTGGAGCTCCGGTCA+TGG - chr2_1:75830371-75830390 None:intergenic 60.0%
GTGCGAGCCAAGTTGCTTGG+TGG + chr2_1:75831501-75831520 Msa0225680:CDS 60.0%
GCAATAGCTGGCCCTTTCCG+GGG - chr2_1:75831856-75831875 None:intergenic 60.0%
GGCAACCTGTGTTGTGGCTG+GGG - chr2_1:75832317-75832336 None:intergenic 60.0%
GAGGCAACCTGTGTTGTGGC+TGG - chr2_1:75832319-75832338 None:intergenic 60.0%
GGCACACTGCCTTCCGATAG+TGG + chr2_1:75833425-75833444 Msa0225680:intron 60.0%
CGGGTGCGAGCCAAGTTGCT+TGG + chr2_1:75831498-75831517 Msa0225680:CDS 65.0%
GTGGACCCCAGCCACAACAC+AGG + chr2_1:75832309-75832328 Msa0225680:CDS 65.0%
! GCAACTTGGCTCGCACCCGC+CGG - chr2_1:75831497-75831516 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 75828873 75834153 75828873 ID=Msa0225680;Name=Msa0225680
chr2_1 mRNA 75828873 75834153 75828873 ID=Msa0225680-mRNA-1;Parent=Msa0225680;Name=Msa0225680-mRNA-1;_AED=0.02;_eAED=0.02;_QI=424|1|1|1|0.8|0.66|6|241|710
chr2_1 exon 75828873 75829156 75828873 ID=Msa0225680-mRNA-1:exon:22765;Parent=Msa0225680-mRNA-1
chr2_1 exon 75829435 75830589 75829435 ID=Msa0225680-mRNA-1:exon:22766;Parent=Msa0225680-mRNA-1
chr2_1 exon 75831474 75832062 75831474 ID=Msa0225680-mRNA-1:exon:22767;Parent=Msa0225680-mRNA-1
chr2_1 exon 75832156 75832378 75832156 ID=Msa0225680-mRNA-1:exon:22768;Parent=Msa0225680-mRNA-1
chr2_1 exon 75832980 75833036 75832980 ID=Msa0225680-mRNA-1:exon:22769;Parent=Msa0225680-mRNA-1
chr2_1 exon 75833664 75834153 75833664 ID=Msa0225680-mRNA-1:exon:22770;Parent=Msa0225680-mRNA-1
chr2_1 five_prime_UTR 75828873 75829156 75828873 ID=Msa0225680-mRNA-1:five_prime_utr;Parent=Msa0225680-mRNA-1
chr2_1 five_prime_UTR 75829435 75829574 75829435 ID=Msa0225680-mRNA-1:five_prime_utr;Parent=Msa0225680-mRNA-1
chr2_1 CDS 75829575 75830589 75829575 ID=Msa0225680-mRNA-1:cds;Parent=Msa0225680-mRNA-1
chr2_1 CDS 75831474 75832062 75831474 ID=Msa0225680-mRNA-1:cds;Parent=Msa0225680-mRNA-1
chr2_1 CDS 75832156 75832378 75832156 ID=Msa0225680-mRNA-1:cds;Parent=Msa0225680-mRNA-1
chr2_1 CDS 75832980 75833036 75832980 ID=Msa0225680-mRNA-1:cds;Parent=Msa0225680-mRNA-1
chr2_1 CDS 75833664 75833912 75833664 ID=Msa0225680-mRNA-1:cds;Parent=Msa0225680-mRNA-1
chr2_1 three_prime_UTR 75833913 75834153 75833913 ID=Msa0225680-mRNA-1:three_prime_utr;Parent=Msa0225680-mRNA-1
Gene Sequence

>Msa0225680

ATGATTGTTGAGGAAAGGAATATAATGGAGGAAAGCAGCAATAGAGGTGAATTTCAGGCTAGTTTAGCGAAACTATCTTCATTAAGATCAGGTGGAAGTTTTAAATCAACATTATCAGGAAGATCGTCTCCGCGAAATAATTCTCCTTCGTTTCGAAGACTAAACTCTAGTCGAACGCCGAGGAAAGAAGGAAGAACTTTTGGTGGTTTTCTGTTGTTTCGGAGCAATCGTGTGTTGTTGTGGCTGCTTCTAATTACCCTCTGGGCTTATCTTGGTATTTTTTTTCAGTCCAGGTGGGCACATATTGATAAGAAGGAGGAATTTTCTGGCTTTGGAACCGGGCCAAGAAATACCGGCGTAGATGCTGATCCGAGTCTTCGCCGTGATTTGATTGCTAGTGATGACTCCTTGTCTGTTAATAATGAGACGGTTAGAAATAAAGGAGGGATTGGTAGAACAATAAATGTAGCCTTGGCAAAGAAGGAAAGTGATGATGAGGTTCCTTCTCGTCGTAAAGCAAGCCCAAAGAAGAAGAGCAGGAGATCTTCGAAAGGTAAAGCTCGTGGTAAGAAGAAACCAATTGTGGAAATTAAGAACAATGATATAGAAGAGCAGGAACCAGAAATTCCTCAAACCAATAGTACGTATGGATTACTCGTCGGTCCATTTGGCTCAACAGAAGATAGGATTTTGGAATGGAGTCCTCAGAAACGTTCCGGGACATGTAATAGGAAGGGGGACTTTGCACGCCTTGTTTGGTCGAGAAGATTTATCTTGATATTCCACGAGCTTTCCATGACCGGAGCTCCACTCTCAATGTTGGAATTGGCAACAGAGCTTTTGAGCTGTGGTGCCACTGTTTCAGCTGTTGCACTTAGCAGGAAGGGTGGTTTGATGTCAGAGCTTTCTAGAAGACGAATCAAGGTGCTTGAGGACAAAGCAGATCAAAGCTTCAAAACTGCAATGAAGGCTGATCTTGTAATTGCTGGTTCAGCTGTATGTGCATCATGGATTGAACAATATATTGAACGTTTTCCGGCGGGTGCGAGCCAAGTTGCTTGGTGGGTCATGGAAAACCGTAGAGAGTACTTCGACCGTTCAAAGGGTGTCTTGCACAGAGTAAAGATGTTGGTTTTTCTTTCTGAATCACAATCTAAACAATGGCAAAAGTGGTGCGAAGAAGAAAACATAAAACTGAGATACCAACCTGCAACTGTTCCTTTGTCTGTTAATGATGAACTGGCATTTGTAGCCGGCATTCCTTCTACACTTAACACTCCGTCCTTTAGTACTGATAAAATGATTGAGAAAAGGCAGTTGTTGCGAGAATCGGTCCGAAAAGAAATGGGATTAACTGACAATGATATGCTTGTAATATCTCTAAGTAGCATCAACCCCGGAAAGGGCCAGCTATTGCTTCTTGAATCAGCAAGCTCAGTAGTAGAACACAAACAATTGCAAGATGATAAGAACATGAAAAAATTATCAAATATAAAGGAAGGCTTGTCTACACGGACTAGAAGACATCGTATGAGAAAATTGTTACCATTGTTGAAAGATGGCAAAGTAGCTCTGAAGGATACCTCAAACAATTCTTTAAGCAGAAGGAAGCAATTGTTGGCTAATAACAAAGCAACAATGCAGCAATCTCTCAAAGTTCTCATTGGTTCTGTTGGATCCAAAAGTAACAAGGTGGATTATGTTAAAAGCCTTCTCAGTTTCTTAGAACAGCATCCAAACACTTCAAAATCAGTTTTGTGGACCCCAGCCACAACACAGGTTGCCTCATTATACTCTGCAGCAGATGTTTATGCCATAAACTCTCAGGGACTTGGAGAGACATTTGGACGGGTGACTATAGAAGCAATGGCATTTGGTCTTCCGGTTCTCGGAACGGATTCTGGGGGAACGAAGGAGATAGTTGAGAACAATGTTACAGGTCTTCTTCATCCAATTGGACGTGCAGCAGGGAACGATGTCCTTGCACGGAATCTTCTGTATTTGCTTAAAAACCAGTTGGCAAGGAAGCAAATGGGGATAGAAGGCAGGAAAAAGGTGGAGAGAATGTATTTGAAGCAACACATGTATAAGAAATTTGTTGAGGTTATTGTTAGGTGCATGAGAAGCAAATAA
Protein sequence

>Msa0225680

MIVEERNIMEESSNRGEFQASLAKLSSLRSGGSFKSTLSGRSSPRNNSPSFRRLNSSRTPRKEGRTFGGFLLFRSNRVLLWLLLITLWAYLGIFFQSRWAHIDKKEEFSGFGTGPRNTGVDADPSLRRDLIASDDSLSVNNETVRNKGGIGRTINVALAKKESDDEVPSRRKASPKKKSRRSSKGKARGKKKPIVEIKNNDIEEQEPEIPQTNSTYGLLVGPFGSTEDRILEWSPQKRSGTCNRKGDFARLVWSRRFILIFHELSMTGAPLSMLELATELLSCGATVSAVALSRKGGLMSELSRRRIKVLEDKADQSFKTAMKADLVIAGSAVCASWIEQYIERFPAGASQVAWWVMENRREYFDRSKGVLHRVKMLVFLSESQSKQWQKWCEEENIKLRYQPATVPLSVNDELAFVAGIPSTLNTPSFSTDKMIEKRQLLRESVRKEMGLTDNDMLVISLSSINPGKGQLLLLESASSVVEHKQLQDDKNMKKLSNIKEGLSTRTRRHRMRKLLPLLKDGKVALKDTSNNSLSRRKQLLANNKATMQQSLKVLIGSVGSKSNKVDYVKSLLSFLEQHPNTSKSVLWTPATTQVASLYSAADVYAINSQGLGETFGRVTIEAMAFGLPVLGTDSGGTKEIVENNVTGLLHPIGRAAGNDVLARNLLYLLKNQLARKQMGIEGRKKVERMYLKQHMYKKFVEVIVRCMRSK*