AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0970230


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0970230 MtrunA17_Chr6g0470291 94.898 98 5 0 1 98 1 98 2.80e-56 169
Msa0970230 MtrunA17_Chr7g0251191 53.933 89 41 0 10 98 6 94 2.36e-35 115
Msa0970230 MtrunA17_Chr6g0483691 49.383 81 41 0 18 98 12 92 3.92e-29 100
Msa0970230 MtrunA17_Chr1g0209271 42.222 90 52 0 9 98 3 92 1.21e-26 94.0
Msa0970230 MtrunA17_Chr7g0251171 42.045 88 51 0 10 97 6 93 1.43e-22 83.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0970230 AT3G18280.2 49.438 89 42 1 10 98 35 120 2.45e-30 104
Msa0970230 AT3G18280.1 49.438 89 42 1 10 98 11 96 4.20e-30 103
Msa0970230 AT1G48750.1 55.556 72 32 0 27 98 23 94 1.47e-28 99.4
Msa0970230 AT1G73780.1 34.694 98 64 0 1 98 1 98 2.78e-22 83.6
Msa0970230 AT1G66850.1 50.725 69 34 0 30 98 34 102 2.02e-21 81.3
Msa0970230 AT1G43666.1 46.154 91 43 3 8 98 11 95 3.82e-20 77.8
Msa0970230 AT5G38170.1 45.588 68 37 0 31 98 36 103 4.62e-19 75.5
Msa0970230 AT5G38195.1 47.761 67 35 0 32 98 29 95 9.17e-19 74.3
Msa0970230 AT1G43667.1 35.000 100 61 2 1 98 1 98 1.87e-18 73.6
Msa0970230 AT5G38160.1 40.260 77 42 1 22 98 31 103 9.75e-18 72.0
Msa0970230 AT5G38180.1 36.000 100 57 2 1 98 1 95 1.09e-17 71.6
Msa0970230 AT2G14846.1 45.161 62 34 0 37 98 38 99 1.31e-14 63.9
Msa0970230 AT1G43665.1 36.250 80 45 2 17 96 22 95 2.29e-14 63.9
Msa0970230 AT1G07747.1 40.909 66 37 1 33 98 29 92 2.30e-13 60.8
Msa0970230 AT5G38197.1 34.694 98 62 2 1 98 1 96 4.62e-13 60.1
Msa0970230 AT3G57310.1 36.364 66 42 0 33 98 38 103 1.05e-12 59.3
Msa0970230 AT4G12825.1 34.066 91 50 2 16 98 7 95 1.67e-12 58.5

Find 28 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAGAGTTCAGTCCTTGTTT+AGG 0.334416 6_4:-1671567 Msa0970230:CDS
GTCTCTGCTCTCTTAACTTC+TGG 0.337118 6_4:+1671505 None:intergenic
TTTACTCTCCTGGTGCTAGA+AGG 0.339135 6_4:-1671437 Msa0970230:CDS
GAGCAGAGACCATGCCTTTG+TGG 0.411218 6_4:-1671492 Msa0970230:CDS
GAAGGGTTACTAGCTCTTGT+GGG 0.413579 6_4:-1671419 Msa0970230:CDS
TTCTGGCAACAAGCACTTGT+TGG 0.433827 6_4:+1671522 None:intergenic
ATGATTGATGGCTAACAAGT+TGG 0.442427 6_4:+1671387 None:intergenic
TAAATATACTGCTTGAGGCT+TGG 0.456606 6_4:+1671456 None:intergenic
AAACAAGGACTGAACTCTAC+AGG 0.466330 6_4:+1671570 None:intergenic
TGGCAACAAGCACTTGTTGG+TGG 0.497936 6_4:+1671525 None:intergenic
AGAAGGGTTACTAGCTCTTG+TGG 0.498825 6_4:-1671420 Msa0970230:CDS
TGATTGATGGCTAACAAGTT+GGG 0.507677 6_4:+1671388 None:intergenic
TGTTTATGCAGTAGTGGTTG+TGG 0.508459 6_4:-1671646 Msa0970230:CDS
AAGCAGTATATTTACTCTCC+TGG 0.517554 6_4:-1671447 Msa0970230:CDS
TGGTATTGTTTATGCAGTAG+TGG 0.524181 6_4:-1671652 Msa0970230:CDS
CTAGTAACCCTTCTAGCACC+AGG 0.531212 6_4:+1671429 None:intergenic
TTACTCTCCTGGTGCTAGAA+GGG 0.532631 6_4:-1671436 Msa0970230:CDS
GCTTGAGGCTTGGATTCTTG+AGG 0.548134 6_4:+1671466 None:intergenic
ATGAAGAAACACTCTAGCTA+TGG 0.561126 6_4:-1671672 Msa0970230:CDS
TGATGGCTAACAAGTTGGGA+AGG 0.576643 6_4:+1671392 None:intergenic
GAGAGTAAATATACTGCTTG+AGG 0.582416 6_4:+1671451 None:intergenic
AAGGGTTACTAGCTCTTGTG+GGG 0.597115 6_4:-1671418 Msa0970230:CDS
GGAGGAGACTTACATGGCAA+AGG 0.624840 6_4:-1671607 Msa0970230:CDS
AACAAGGACTGAACTCTACA+GGG 0.629235 6_4:+1671571 None:intergenic
GATGGCTAACAAGTTGGGAA+GGG 0.649759 6_4:+1671393 None:intergenic
TTGAGGTAACCACAAAGGCA+TGG 0.650224 6_4:+1671483 None:intergenic
GATTCTTGAGGTAACCACAA+AGG 0.669776 6_4:+1671478 None:intergenic
GTTGTGATTGATCCTAAACA+AGG 0.684294 6_4:+1671555 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATGAAGAAACACTCTAGCTA+TGG - chr6_4:1671398-1671417 Msa0970230:CDS 35.0%
TGGTATTGTTTATGCAGTAG+TGG - chr6_4:1671418-1671437 Msa0970230:CDS 35.0%
! GTTGTGGCAATAGTGTTTTT+GGG - chr6_4:1671440-1671459 Msa0970230:CDS 35.0%
!! TTGTGGCAATAGTGTTTTTG+GGG - chr6_4:1671441-1671460 Msa0970230:CDS 35.0%
GTTGTGATTGATCCTAAACA+AGG + chr6_4:1671518-1671537 None:intergenic 35.0%
TAAATATACTGCTTGAGGCT+TGG + chr6_4:1671617-1671636 None:intergenic 35.0%
GAGAGTAAATATACTGCTTG+AGG + chr6_4:1671622-1671641 None:intergenic 35.0%
AAGCAGTATATTTACTCTCC+TGG - chr6_4:1671623-1671642 Msa0970230:CDS 35.0%
TGTTTATGCAGTAGTGGTTG+TGG - chr6_4:1671424-1671443 Msa0970230:CDS 40.0%
! GGTTGTGGCAATAGTGTTTT+TGG - chr6_4:1671439-1671458 Msa0970230:CDS 40.0%
!!! TGTGGCAATAGTGTTTTTGG+GGG - chr6_4:1671442-1671461 Msa0970230:CDS 40.0%
AACAAGGACTGAACTCTACA+GGG + chr6_4:1671502-1671521 None:intergenic 40.0%
AAACAAGGACTGAACTCTAC+AGG + chr6_4:1671503-1671522 None:intergenic 40.0%
! GTAGAGTTCAGTCCTTGTTT+AGG - chr6_4:1671503-1671522 Msa0970230:CDS 40.0%
GATTCTTGAGGTAACCACAA+AGG + chr6_4:1671595-1671614 None:intergenic 40.0%
TTCTGGCAACAAGCACTTGT+TGG + chr6_4:1671551-1671570 None:intergenic 45.0%
GTCTCTGCTCTCTTAACTTC+TGG + chr6_4:1671568-1671587 None:intergenic 45.0%
TTGAGGTAACCACAAAGGCA+TGG + chr6_4:1671590-1671609 None:intergenic 45.0%
! TTTACTCTCCTGGTGCTAGA+AGG - chr6_4:1671633-1671652 Msa0970230:CDS 45.0%
! TTACTCTCCTGGTGCTAGAA+GGG - chr6_4:1671634-1671653 Msa0970230:CDS 45.0%
! AGAAGGGTTACTAGCTCTTG+TGG - chr6_4:1671650-1671669 Msa0970230:CDS 45.0%
! GAAGGGTTACTAGCTCTTGT+GGG - chr6_4:1671651-1671670 Msa0970230:CDS 45.0%
! AAGGGTTACTAGCTCTTGTG+GGG - chr6_4:1671652-1671671 Msa0970230:CDS 45.0%
!!! GGCAATAGTGTTTTTGGGGG+AGG - chr6_4:1671445-1671464 Msa0970230:CDS 50.0%
TTTGGGGGAGGAGACTTACA+TGG - chr6_4:1671457-1671476 Msa0970230:CDS 50.0%
GGAGGAGACTTACATGGCAA+AGG - chr6_4:1671463-1671482 Msa0970230:CDS 50.0%
! TGGCAACAAGCACTTGTTGG+TGG + chr6_4:1671548-1671567 None:intergenic 50.0%
GCTTGAGGCTTGGATTCTTG+AGG + chr6_4:1671607-1671626 None:intergenic 50.0%
CTAGTAACCCTTCTAGCACC+AGG + chr6_4:1671644-1671663 None:intergenic 50.0%
GAGCAGAGACCATGCCTTTG+TGG - chr6_4:1671578-1671597 Msa0970230:CDS 55.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 1671398 1671694 1671398 ID=Msa0970230;Name=Msa0970230
chr6_4 mRNA 1671398 1671694 1671398 ID=Msa0970230-mRNA-1;Parent=Msa0970230;Name=Msa0970230-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|98
chr6_4 exon 1671398 1671694 1671398 ID=Msa0970230-mRNA-1:exon:844;Parent=Msa0970230-mRNA-1
chr6_4 CDS 1671398 1671694 1671398 ID=Msa0970230-mRNA-1:cds;Parent=Msa0970230-mRNA-1
Gene Sequence

>Msa0970230

ATGAAGAAACACTCTAGCTATGGTATTGTTTATGCAGTAGTGGTTGTGGCAATAGTGTTTTTGGGGGAGGAGACTTACATGGCAAAGGCATTGACTTGTAGCCCTGTAGAGTTCAGTCCTTGTTTAGGATCAATCACAACTTCTTCTCCACCAACAAGTGCTTGTTGCCAGAAGTTAAGAGAGCAGAGACCATGCCTTTGTGGTTACCTCAAGAATCCAAGCCTCAAGCAGTATATTTACTCTCCTGGTGCTAGAAGGGTTACTAGCTCTTGTGGGGTTCCCTTCCCAACTTGTTAG

Protein sequence

>Msa0970230

MKKHSSYGIVYAVVVVAIVFLGEETYMAKALTCSPVEFSPCLGSITTSSPPTSACCQKLREQRPCLCGYLKNPSLKQYIYSPGARRVTSSCGVPFPTC*