AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0674960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0674960 MtrunA17_Chr4g0018191 92.262 168 12 1 1 168 30 196 1.23e-103 295
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0674960 AT5G51545.1 41.176 187 81 4 1 164 1 181 6.50e-38 128

Find 58 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGAAATTGTCCCACTCTTC+TGG 0.220374 4_4:+23069881 None:intergenic
CGATGATTGCAATCCTATTC+TGG 0.230842 4_4:-23069685 Msa0674960:three_prime_UTR
CAATCCCATTTGTAATTAAC+TGG 0.242118 4_4:-23069536 Msa0674960:three_prime_UTR
TCATCTGTCATCTTCGATTC+TGG 0.265141 4_4:+23069652 None:intergenic
GGTATTGCTCTTGCTGCTTC+AGG 0.275480 4_4:-23072205 Msa0674960:intron
CTCGGCCATATGATGCAATA+TGG 0.279745 4_4:+23069583 None:intergenic
AACAAAACTGGTGTGGGTTT+TGG 0.286345 4_4:-23072430 Msa0674960:CDS
ACAGTATTATTGTTTCTTGC+TGG 0.296795 4_4:-23069827 Msa0674960:CDS
ATTCTTGCGTGGCTTGGTTT+TGG 0.298906 4_4:-23072247 Msa0674960:CDS
GAGAAAGAGCTTCGATAATT+CGG 0.322344 4_4:-23072363 Msa0674960:CDS
CTTGCTGGAGCAGTAGCATT+TGG 0.343726 4_4:-23069812 Msa0674960:CDS
CTTCTTAACCTGTGCCTTGA+GGG 0.369589 4_4:+23069430 None:intergenic
TCGGACTTGATTGTGAAGAT+AGG 0.376155 4_4:+23072484 None:intergenic
GCAATATGGGGTTGCTGCTT+AGG 0.394933 4_4:+23069597 None:intergenic
GGTTCGGTTATTCTTGTTGA+GGG 0.408372 4_4:-23072226 Msa0674960:CDS
GTAATCCAGTTAATTACAAA+TGG 0.412509 4_4:+23069531 None:intergenic
TTGCCCTTTCTGTTTCCGTT+TGG 0.419619 4_4:+23072387 None:intergenic
CAACCCCATATTGCATCATA+TGG 0.423728 4_4:-23069588 Msa0674960:three_prime_UTR
GATTCAGTTCCCAACAAAAC+TGG 0.426754 4_4:-23072442 Msa0674960:CDS
AGAGAATGAGTGATGTAGTT+TGG 0.434413 4_4:+23072507 None:intergenic
GAAGAGAGTGCTGTTGAGAA+AGG 0.436447 4_4:-23072274 Msa0674960:CDS
TGGCAAATTGAAGCCAGAAT+AGG 0.436939 4_4:+23069672 None:intergenic
ATCATATGGCCGAGTAACTA+AGG 0.451496 4_4:-23069574 Msa0674960:three_prime_UTR
GGTTTCATTCTTGCGTGGCT+TGG 0.452705 4_4:-23072253 Msa0674960:CDS
GAAGATTGAACAATTTATGT+TGG 0.453892 4_4:-23069622 Msa0674960:three_prime_UTR
CAATAATACTGTAGGGGTAA+AGG 0.457025 4_4:+23069838 None:intergenic
ATCTTGTGCCCTCAAGGCAC+AGG 0.481761 4_4:-23069438 Msa0674960:three_prime_UTR
AACACCAAACGGAAACAGAA+AGG 0.495671 4_4:-23072391 Msa0674960:CDS
ACACCAAACGGAAACAGAAA+GGG 0.501372 4_4:-23072390 Msa0674960:CDS
GCAAGAAACAATAATACTGT+AGG 0.502406 4_4:+23069830 None:intergenic
TAATCCAGTTAATTACAAAT+GGG 0.503901 4_4:+23069532 None:intergenic
TCGGCCATATGATGCAATAT+GGG 0.503994 4_4:+23069584 None:intergenic
CAAGAAACAATAATACTGTA+GGG 0.504164 4_4:+23069831 None:intergenic
TGTTCGAAATCTCTAATGTC+CGG 0.506879 4_4:-23069726 Msa0674960:three_prime_UTR
GCTTCTTAACCTGTGCCTTG+AGG 0.509674 4_4:+23069429 None:intergenic
TGGTTCGGTTATTCTTGTTG+AGG 0.511249 4_4:-23072227 Msa0674960:CDS
TTGGTGTTGTTGCCATTCGA+CGG 0.523797 4_4:+23072406 None:intergenic
AATTGTTCCGAAACGAAAGC+CGG 0.532646 4_4:+23072322 None:intergenic
TTTCATAAACCTTAGTTACT+CGG 0.533982 4_4:+23069565 None:intergenic
AATGTTATCTTGTGCCCTCA+AGG 0.551537 4_4:-23069444 Msa0674960:three_prime_UTR
CGAATGGCAACAACACCAAA+CGG 0.552467 4_4:-23072402 Msa0674960:CDS
AATGAGTGATGTAGTTTGGT+GGG 0.564862 4_4:+23072511 None:intergenic
GTGATGTAGTTTGGTGGGGA+AGG 0.567049 4_4:+23072516 None:intergenic
GTTCCCAACAAAACTGGTGT+GGG 0.575858 4_4:-23072436 Msa0674960:CDS
AGTTCCCAACAAAACTGGTG+TGG 0.579102 4_4:-23072437 Msa0674960:CDS
ACTGAATCGGACGGTTCAGA+AGG 0.586590 4_4:+23072457 None:intergenic
AATACTGTAGGGGTAAAGGA+TGG 0.613012 4_4:+23069842 None:intergenic
AGAAAGGTTTCATTCTTGCG+TGG 0.613254 4_4:-23072258 Msa0674960:CDS
TGGAGCTTAGTTTCACCACA+TGG 0.617051 4_4:+23072584 None:intergenic
GAATGAGTGATGTAGTTTGG+TGG 0.628646 4_4:+23072510 None:intergenic
GAGTAACTAAGGTTTATGAA+AGG 0.637597 4_4:-23069563 Msa0674960:three_prime_UTR
CGGCCATATGATGCAATATG+GGG 0.639421 4_4:+23069585 None:intergenic
TAAGCAGAATGAGAAACTCA+CGG 0.646278 4_4:-23069777 Msa0674960:CDS
AAGAAACAATAATACTGTAG+GGG 0.647405 4_4:+23069832 None:intergenic
ATGAGTGATGTAGTTTGGTG+GGG 0.673147 4_4:+23072512 None:intergenic
TTGTTGGGAACTGAATCGGA+CGG 0.702263 4_4:+23072448 None:intergenic
ATGGATAGAGATACTTGACA+AGG 0.707055 4_4:+23069861 None:intergenic
TGGAGCAGTAGCATTTGGAG+TGG 0.720186 4_4:-23069807 Msa0674960:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAAGATAAATAATGATA+GGG - chr4_4:23070281-23070300 Msa0674960:intron 10.0%
!! TTAATACTATTTATTATTCA+AGG - chr4_4:23071816-23071835 Msa0674960:intron 10.0%
!!! TTTTTATTAAATTATGTACT+AGG - chr4_4:23072516-23072535 Msa0674960:CDS 10.0%
!!! TAAAAATAGAAATATTTTGT+TGG - chr4_4:23072615-23072634 Msa0674960:five_prime_UTR 10.0%
!!! TATTTTTCAATTAAGGTTAT+AGG - chr4_4:23070254-23070273 Msa0674960:intron 15.0%
!!! ATTTTTCAATTAAGGTTATA+GGG - chr4_4:23070255-23070274 Msa0674960:intron 15.0%
!! GAATTAAGATAAATAATGAT+AGG - chr4_4:23070280-23070299 Msa0674960:intron 15.0%
!!! TTAGTTTAAATCTTTTGTTT+TGG - chr4_4:23070547-23070566 Msa0674960:intron 15.0%
!!! TATTTTGTCATTTTTTACAT+AGG + chr4_4:23071047-23071066 None:intergenic 15.0%
!! ATGTTCATTTAAAAAATTTG+AGG - chr4_4:23071861-23071880 Msa0674960:intron 15.0%
!! AGAAATGAAAAACAATAATT+TGG + chr4_4:23071888-23071907 None:intergenic 15.0%
!! TTGCAATTAGAGTAAATTAA+GGG - chr4_4:23069919-23069938 Msa0674960:intron 20.0%
!!! TTTTTCAATTAAGGTTATAG+GGG - chr4_4:23070256-23070275 Msa0674960:intron 20.0%
!! TTGAAGTAGTTTATCAAAAA+TGG - chr4_4:23070328-23070347 Msa0674960:intron 20.0%
!! GATCTTTACTCTAAATTTAA+AGG + chr4_4:23070908-23070927 None:intergenic 20.0%
!!! ATTAGAATTTAGGATCTAAA+TGG - chr4_4:23071445-23071464 Msa0674960:intron 20.0%
!!! TTAGAATTTAGGATCTAAAT+GGG - chr4_4:23071446-23071465 Msa0674960:intron 20.0%
!! TTTGTTGAAATTAATGATTG+AGG - chr4_4:23071491-23071510 Msa0674960:intron 20.0%
!! TAATCCAGTTAATTACAAAT+GGG + chr4_4:23072492-23072511 None:intergenic 20.0%
!! TCTACACATAAAAATCTTAT+GGG - chr4_4:23072553-23072572 Msa0674960:exon 20.0%
! GTTGCAATTAGAGTAAATTA+AGG - chr4_4:23069918-23069937 Msa0674960:intron 25.0%
! GATAAACCAAACTATTATAG+TGG - chr4_4:23070008-23070027 Msa0674960:intron 25.0%
!! TTTTCAATTAAGGTTATAGG+GGG - chr4_4:23070257-23070276 Msa0674960:intron 25.0%
! AGATAGTAAGACATAACTAT+AGG - chr4_4:23070413-23070432 Msa0674960:intron 25.0%
!!! CTAATAAGCTGTTTTCTTAA+GGG + chr4_4:23070465-23070484 None:intergenic 25.0%
!!! TCTAATAAGCTGTTTTCTTA+AGG + chr4_4:23070466-23070485 None:intergenic 25.0%
! ATAAGAGATTATGCTATAAG+CGG - chr4_4:23070606-23070625 Msa0674960:intron 25.0%
!! GGAAGTATATATGTTAGAAT+AGG - chr4_4:23070791-23070810 Msa0674960:intron 25.0%
!! TAGTGTTGAAAGTTTGAATA+TGG - chr4_4:23071108-23071127 Msa0674960:intron 25.0%
!! TGTATTTGAATGCTATGTTT+TGG - chr4_4:23071385-23071404 Msa0674960:intron 25.0%
!! GTATTTGAATGCTATGTTTT+GGG - chr4_4:23071386-23071405 Msa0674960:intron 25.0%
!!! GGATTTATTTTTATTCTCTC+TGG - chr4_4:23071625-23071644 Msa0674960:intron 25.0%
! AGAGACTATCAATTACAATA+TGG - chr4_4:23071692-23071711 Msa0674960:intron 25.0%
! GAGACTATCAATTACAATAT+GGG - chr4_4:23071693-23071712 Msa0674960:intron 25.0%
!! ACATTGATACTTTCATTTTC+AGG + chr4_4:23071768-23071787 None:intergenic 25.0%
!!! TAACTCGTTCTTTAGAATAA+AGG + chr4_4:23071963-23071982 None:intergenic 25.0%
! GTAAAATGACACTAATTCTT+AGG + chr4_4:23072074-23072093 None:intergenic 25.0%
! AAGAAACAATAATACTGTAG+GGG + chr4_4:23072192-23072211 None:intergenic 25.0%
! CAAGAAACAATAATACTGTA+GGG + chr4_4:23072193-23072212 None:intergenic 25.0%
! GAAGATTGAACAATTTATGT+TGG - chr4_4:23072399-23072418 Msa0674960:CDS 25.0%
! TTTCATAAACCTTAGTTACT+CGG + chr4_4:23072459-23072478 None:intergenic 25.0%
! GTAATCCAGTTAATTACAAA+TGG + chr4_4:23072493-23072512 None:intergenic 25.0%
!! CATAAGATTTTTATGTGTAG+AGG + chr4_4:23072554-23072573 None:intergenic 25.0%
! CTCTACACATAAAAATCTTA+TGG - chr4_4:23072552-23072571 Msa0674960:exon 25.0%
ATCAGAAAAAAGAAACCATG+TGG - chr4_4:23069422-23069441 Msa0674960:three_prime_UTR 30.0%
!!! CATTTTTGTTTTGTTGGAGT+TGG + chr4_4:23069460-23069479 None:intergenic 30.0%
CAACTCCAACAAAACAAAAA+TGG - chr4_4:23069458-23069477 Msa0674960:three_prime_UTR 30.0%
TTTGAGCTAAACTTGTAAGA+CGG - chr4_4:23069961-23069980 Msa0674960:intron 30.0%
AAACATTACTAAACCCACTT+GGG + chr4_4:23070158-23070177 None:intergenic 30.0%
TTTCAATTAAGGTTATAGGG+GGG - chr4_4:23070258-23070277 Msa0674960:intron 30.0%
ATAACTATAGGCTCAAAAAC+AGG - chr4_4:23070425-23070444 Msa0674960:intron 30.0%
! TGTTTTCTTAAGGGTCTTTT+TGG + chr4_4:23070456-23070475 None:intergenic 30.0%
AAGTTATCCTAGAAAGCTTA+TGG + chr4_4:23070516-23070535 None:intergenic 30.0%
! TCGTACTTCTCTTTCTTTTA+GGG + chr4_4:23070681-23070700 None:intergenic 30.0%
! TTCGTACTTCTCTTTCTTTT+AGG + chr4_4:23070682-23070701 None:intergenic 30.0%
GTTCTATTGAAGTTTAGAGA+TGG - chr4_4:23071075-23071094 Msa0674960:intron 30.0%
TTTGAATATGGCTGAAGAAT+TGG - chr4_4:23071120-23071139 Msa0674960:intron 30.0%
GTCCTTTCTATTACCAATTT+GGG + chr4_4:23071256-23071275 None:intergenic 30.0%
ACAGAATTTACCAGATTATC+CGG + chr4_4:23071279-23071298 None:intergenic 30.0%
!! TTGTGTTAATGCTCTGAAAA+GGG + chr4_4:23071419-23071438 None:intergenic 30.0%
ACTATCAATTACAATATGGG+AGG - chr4_4:23071696-23071715 Msa0674960:intron 30.0%
TGGGAGGAAAAATAAAGTAT+CGG - chr4_4:23071712-23071731 Msa0674960:intron 30.0%
CTGATTAAGATACAATAGCT+TGG + chr4_4:23072024-23072043 None:intergenic 30.0%
! CTTAATCAGTTAGTTGACTT+AGG - chr4_4:23072035-23072054 Msa0674960:intron 30.0%
GCAAGAAACAATAATACTGT+AGG + chr4_4:23072194-23072213 None:intergenic 30.0%
! ACAGTATTATTGTTTCTTGC+TGG - chr4_4:23072194-23072213 Msa0674960:intron 30.0%
GAGTAACTAAGGTTTATGAA+AGG - chr4_4:23072458-23072477 Msa0674960:CDS 30.0%
CAATCCCATTTGTAATTAAC+TGG - chr4_4:23072485-23072504 Msa0674960:CDS 30.0%
!!! TAGCGCCATTTTTGTTTTGT+TGG + chr4_4:23069466-23069485 None:intergenic 35.0%
AATGAGTGATGTAGTTTGGT+GGG + chr4_4:23069513-23069532 None:intergenic 35.0%
AGAGAATGAGTGATGTAGTT+TGG + chr4_4:23069517-23069536 None:intergenic 35.0%
!!! AGTTTTGTTGGGAACTGAAT+CGG + chr4_4:23069580-23069599 None:intergenic 35.0%
GAGAAAGAGCTTCGATAATT+CGG - chr4_4:23069658-23069677 Msa0674960:three_prime_UTR 35.0%
GAGTAAATTAAGGGTCTGTT+TGG - chr4_4:23069928-23069947 Msa0674960:intron 35.0%
AATTAAGGGTCTGTTTGGAT+TGG - chr4_4:23069933-23069952 Msa0674960:intron 35.0%
GCTCATCCACTATAATAGTT+TGG + chr4_4:23070017-23070036 None:intergenic 35.0%
TCGGTTATAAGCAAGCTTAT+CGG + chr4_4:23070053-23070072 None:intergenic 35.0%
! AGCTTGCTTATAACCGATAA+CGG - chr4_4:23070056-23070075 Msa0674960:intron 35.0%
CAAACATTACTAAACCCACT+TGG + chr4_4:23070159-23070178 None:intergenic 35.0%
! CAAGTGGGTTTAGTAATGTT+TGG - chr4_4:23070157-23070176 Msa0674960:intron 35.0%
ATAAGCTTGTGAGAAAGTGA+CGG - chr4_4:23070353-23070372 Msa0674960:intron 35.0%
CGTATTTCCATAAGCTTTCT+AGG - chr4_4:23070506-23070525 Msa0674960:intron 35.0%
GTACGAATTTGCAATCAACA+TGG - chr4_4:23070695-23070714 Msa0674960:intron 35.0%
GATTTGATTAGCAGTGTGTA+TGG - chr4_4:23070770-23070789 Msa0674960:intron 35.0%
!! GAAACTAGTCTCTGATTTTC+TGG - chr4_4:23070847-23070866 Msa0674960:intron 35.0%
CGTCACTTCTTAAAGAGTTT+AGG - chr4_4:23071002-23071021 Msa0674960:intron 35.0%
GGTCCTTTCTATTACCAATT+TGG + chr4_4:23071257-23071276 None:intergenic 35.0%
! AATTGGTAATAGAAAGGACC+CGG - chr4_4:23071257-23071276 Msa0674960:intron 35.0%
CAGAATTTACCAGATTATCC+GGG + chr4_4:23071278-23071297 None:intergenic 35.0%
!!! TGAATGCTATGTTTTGGGTT+AGG - chr4_4:23071391-23071410 Msa0674960:intron 35.0%
!! TGTGTTAATGCTCTGAAAAG+GGG + chr4_4:23071418-23071437 None:intergenic 35.0%
!! GTTGTGTTAATGCTCTGAAA+AGG + chr4_4:23071420-23071439 None:intergenic 35.0%
ACAACCAGGGATTAGAATTT+AGG - chr4_4:23071435-23071454 Msa0674960:intron 35.0%
AGATCCTAAATTCTAATCCC+TGG + chr4_4:23071442-23071461 None:intergenic 35.0%
CTAACTCTTCTTCTTCTTCA+TGG + chr4_4:23071564-23071583 None:intergenic 35.0%
ACTCTCGATATGTTGTCAAT+AGG + chr4_4:23071599-23071618 None:intergenic 35.0%
GACACTAATTCTTAGGTTCT+AGG + chr4_4:23072067-23072086 None:intergenic 35.0%
!! GTCATTTTACATCGATACTG+TGG - chr4_4:23072084-23072103 Msa0674960:intron 35.0%
CGATACTGTGGTTAATACTT+TGG - chr4_4:23072096-23072115 Msa0674960:intron 35.0%
! ATACTTTGGTGTGTTATTGC+AGG - chr4_4:23072110-23072129 Msa0674960:intron 35.0%
ATGGATAGAGATACTTGACA+AGG + chr4_4:23072163-23072182 None:intergenic 35.0%
CAATAATACTGTAGGGGTAA+AGG + chr4_4:23072186-23072205 None:intergenic 35.0%
TAAGCAGAATGAGAAACTCA+CGG - chr4_4:23072244-23072263 Msa0674960:CDS 35.0%
TGTTCGAAATCTCTAATGTC+CGG - chr4_4:23072295-23072314 Msa0674960:CDS 35.0%
!!! GTCACTCATAGTACTTTTTC+CGG + chr4_4:23072317-23072336 None:intergenic 35.0%
! ATGAGTGATGTAGTTTGGTG+GGG + chr4_4:23069512-23069531 None:intergenic 40.0%
GAATGAGTGATGTAGTTTGG+TGG + chr4_4:23069514-23069533 None:intergenic 40.0%
TCGGACTTGATTGTGAAGAT+AGG + chr4_4:23069540-23069559 None:intergenic 40.0%
GATTCAGTTCCCAACAAAAC+TGG - chr4_4:23069579-23069598 Msa0674960:three_prime_UTR 40.0%
!!! AAACCCACACCAGTTTTGTT+GGG + chr4_4:23069591-23069610 None:intergenic 40.0%
!! AAAACCCACACCAGTTTTGT+TGG + chr4_4:23069592-23069611 None:intergenic 40.0%
! AACAAAACTGGTGTGGGTTT+TGG - chr4_4:23069591-23069610 Msa0674960:three_prime_UTR 40.0%
AACACCAAACGGAAACAGAA+AGG - chr4_4:23069630-23069649 Msa0674960:three_prime_UTR 40.0%
ACACCAAACGGAAACAGAAA+GGG - chr4_4:23069631-23069650 Msa0674960:three_prime_UTR 40.0%
GCGTAATCCAGTTGAAAAAC+CGG - chr4_4:23069680-23069699 Msa0674960:three_prime_UTR 40.0%
! TGAAAAACCGGCTTTCGTTT+CGG - chr4_4:23069692-23069711 Msa0674960:three_prime_UTR 40.0%
AATTGTTCCGAAACGAAAGC+CGG + chr4_4:23069702-23069721 None:intergenic 40.0%
AGAAAGGTTTCATTCTTGCG+TGG - chr4_4:23069763-23069782 Msa0674960:exon 40.0%
!! TGGTTCGGTTATTCTTGTTG+AGG - chr4_4:23069794-23069813 Msa0674960:CDS 40.0%
! GGTTCGGTTATTCTTGTTGA+GGG - chr4_4:23069795-23069814 Msa0674960:CDS 40.0%
GCTAAACTTGTAAGACGGTT+TGG - chr4_4:23069966-23069985 Msa0674960:intron 40.0%
! TCAGCTAGCTTATCCGTTAT+CGG + chr4_4:23070072-23070091 None:intergenic 40.0%
TTGGAGTATGCTCTTCCTAT+AGG + chr4_4:23070100-23070119 None:intergenic 40.0%
! TATAGGAAGAGCATACTCCA+AGG - chr4_4:23070099-23070118 Msa0674960:intron 40.0%
GTCTCTTAAGCATGTGGTTT+GGG - chr4_4:23070748-23070767 Msa0674960:intron 40.0%
! GAATAGGTCAGCCTCTTTTA+TGG - chr4_4:23070807-23070826 Msa0674960:intron 40.0%
GGTACGGAAATCCATAAAAG+AGG + chr4_4:23070821-23070840 None:intergenic 40.0%
!! CTAGTCTCTGATTTTCTGGT+TGG - chr4_4:23070851-23070870 Msa0674960:intron 40.0%
!! TTTCTGGTTGGAGGATAACT+TGG - chr4_4:23070863-23070882 Msa0674960:intron 40.0%
! TTCTGGTTGGAGGATAACTT+GGG - chr4_4:23070864-23070883 Msa0674960:intron 40.0%
GTGTCATGTTTGTGCATCAT+AGG + chr4_4:23070935-23070954 None:intergenic 40.0%
GTGACAAAATAACCCAAGTC+CGG - chr4_4:23071185-23071204 Msa0674960:intron 40.0%
ATTACCAATTTGGGCCAACA+GGG + chr4_4:23071247-23071266 None:intergenic 40.0%
TATTACCAATTTGGGCCAAC+AGG + chr4_4:23071248-23071267 None:intergenic 40.0%
! GGCCCAAATTGGTAATAGAA+AGG - chr4_4:23071251-23071270 Msa0674960:intron 40.0%
!! GTGTTAATGCTCTGAAAAGG+GGG + chr4_4:23071417-23071436 None:intergenic 40.0%
! TTCAGAGCATTAACACAACC+AGG - chr4_4:23071421-23071440 Msa0674960:intron 40.0%
! TCAGAGCATTAACACAACCA+GGG - chr4_4:23071422-23071441 Msa0674960:intron 40.0%
! CAACATATCGAGAGTGTGTA+AGG - chr4_4:23071604-23071623 Msa0674960:intron 40.0%
ACTATGACCCAAACCTAAGA+TGG - chr4_4:23071995-23072014 Msa0674960:intron 40.0%
TTGGAGACCATCTTAGGTTT+GGG + chr4_4:23072005-23072024 None:intergenic 40.0%
AATAGCTTGGAGACCATCTT+AGG + chr4_4:23072011-23072030 None:intergenic 40.0%
!!! AGGCTTTTTTCCAGAAGAGT+GGG - chr4_4:23072130-23072149 Msa0674960:intron 40.0%
AATACTGTAGGGGTAAAGGA+TGG + chr4_4:23072182-23072201 None:intergenic 40.0%
CGATGATTGCAATCCTATTC+TGG - chr4_4:23072336-23072355 Msa0674960:CDS 40.0%
TGGCAAATTGAAGCCAGAAT+AGG + chr4_4:23072352-23072371 None:intergenic 40.0%
TCATCTGTCATCTTCGATTC+TGG + chr4_4:23072372-23072391 None:intergenic 40.0%
CAACCCCATATTGCATCATA+TGG - chr4_4:23072433-23072452 Msa0674960:CDS 40.0%
TCGGCCATATGATGCAATAT+GGG + chr4_4:23072440-23072459 None:intergenic 40.0%
ATCATATGGCCGAGTAACTA+AGG - chr4_4:23072447-23072466 Msa0674960:CDS 40.0%
AATGTTATCTTGTGCCCTCA+AGG - chr4_4:23072577-23072596 Msa0674960:five_prime_UTR 40.0%
TGGAGCTTAGTTTCACCACA+TGG + chr4_4:23069440-23069459 None:intergenic 45.0%
! GGACGGTTCAGAAGGTTTTT+CGG + chr4_4:23069559-23069578 None:intergenic 45.0%
TTGTTGGGAACTGAATCGGA+CGG + chr4_4:23069576-23069595 None:intergenic 45.0%
AGTTCCCAACAAAACTGGTG+TGG - chr4_4:23069584-23069603 Msa0674960:three_prime_UTR 45.0%
GTTCCCAACAAAACTGGTGT+GGG - chr4_4:23069585-23069604 Msa0674960:three_prime_UTR 45.0%
!! TTGGTGTTGTTGCCATTCGA+CGG + chr4_4:23069618-23069637 None:intergenic 45.0%
CGAATGGCAACAACACCAAA+CGG - chr4_4:23069619-23069638 Msa0674960:three_prime_UTR 45.0%
TTGCCCTTTCTGTTTCCGTT+TGG + chr4_4:23069637-23069656 None:intergenic 45.0%
!! ACGAAAGCCGGTTTTTCAAC+TGG + chr4_4:23069690-23069709 None:intergenic 45.0%
!! GAAGAGAGTGCTGTTGAGAA+AGG - chr4_4:23069747-23069766 Msa0674960:exon 45.0%
!!! ATTCTTGCGTGGCTTGGTTT+TGG - chr4_4:23069774-23069793 Msa0674960:CDS 45.0%
AGCTAGCTGATGAGACCTAT+AGG - chr4_4:23070082-23070101 Msa0674960:intron 45.0%
GCAATCAACATGGTGTGACA+TGG - chr4_4:23070705-23070724 Msa0674960:intron 45.0%
!! GGTTGACTTGAGAGATAACC+AGG - chr4_4:23070726-23070745 Msa0674960:intron 45.0%
AACCAGGTCTCTTAAGCATG+TGG - chr4_4:23070742-23070761 Msa0674960:intron 45.0%
AACCACATGCTTAAGAGACC+TGG + chr4_4:23070747-23070766 None:intergenic 45.0%
GGTCTCTTAAGCATGTGGTT+TGG - chr4_4:23070747-23070766 Msa0674960:intron 45.0%
!! GACTAGTTTCTCTCGAGGTA+CGG + chr4_4:23070837-23070856 None:intergenic 45.0%
!! TCAGAGACTAGTTTCTCTCG+AGG + chr4_4:23070842-23070861 None:intergenic 45.0%
!!! GTCTCTGATTTTCTGGTTGG+AGG - chr4_4:23070854-23070873 Msa0674960:intron 45.0%
TAGAAAGGACCCGGATAATC+TGG - chr4_4:23071266-23071285 Msa0674960:intron 45.0%
CTTGGAGACCATCTTAGGTT+TGG + chr4_4:23072006-23072025 None:intergenic 45.0%
!!! CAGGCTTTTTTCCAGAAGAG+TGG - chr4_4:23072129-23072148 Msa0674960:intron 45.0%
AGGAAATTGTCCCACTCTTC+TGG + chr4_4:23072143-23072162 None:intergenic 45.0%
CGGCCATATGATGCAATATG+GGG + chr4_4:23072439-23072458 None:intergenic 45.0%
CTCGGCCATATGATGCAATA+TGG + chr4_4:23072441-23072460 None:intergenic 45.0%
CTTCTTAACCTGTGCCTTGA+GGG + chr4_4:23072594-23072613 None:intergenic 45.0%
!! AAGAATTAAAAAAAAAAATA+AGG + chr4_4:23069849-23069868 None:intergenic 5.0%
!!! TTTATTTTATTTTTCAATTA+AGG - chr4_4:23070247-23070266 Msa0674960:intron 5.0%
! GTGATGTAGTTTGGTGGGGA+AGG + chr4_4:23069508-23069527 None:intergenic 50.0%
ACTGAATCGGACGGTTCAGA+AGG + chr4_4:23069567-23069586 None:intergenic 50.0%
GGTTTCATTCTTGCGTGGCT+TGG - chr4_4:23069768-23069787 Msa0674960:CDS 50.0%
!!! TGCGTGGCTTGGTTTTGGTT+CGG - chr4_4:23069779-23069798 Msa0674960:CDS 50.0%
! GGTATTGCTCTTGCTGCTTC+AGG - chr4_4:23069816-23069835 Msa0674960:CDS 50.0%
GCTTATCGGTCATGCGCTAT+AGG + chr4_4:23070039-23070058 None:intergenic 50.0%
!! TAAACCCACTTGGGTTAGCC+TGG + chr4_4:23070149-23070168 None:intergenic 50.0%
CTTGCTGGAGCAGTAGCATT+TGG - chr4_4:23072209-23072228 Msa0674960:CDS 50.0%
TGGAGCAGTAGCATTTGGAG+TGG - chr4_4:23072214-23072233 Msa0674960:CDS 50.0%
! GCAATATGGGGTTGCTGCTT+AGG + chr4_4:23072427-23072446 None:intergenic 50.0%
GCTTCTTAACCTGTGCCTTG+AGG + chr4_4:23072595-23072614 None:intergenic 50.0%
!! GTGGGTTTTGGTCCGTCGAA+TGG - chr4_4:23069603-23069622 Msa0674960:three_prime_UTR 55.0%
! TGCTCTTGCTGCTTCAGGTC+AGG - chr4_4:23069821-23069840 Msa0674960:CDS 55.0%
TCCACCAGGCTAACCCAAGT+GGG - chr4_4:23070142-23070161 Msa0674960:intron 55.0%
TTGGGTTTGGCTGTGTAGGC+CGG + chr4_4:23071207-23071226 None:intergenic 55.0%
GAGGTTGGGTTTGGCTGTGT+AGG + chr4_4:23071211-23071230 None:intergenic 55.0%
ACAGCCAAACCCAACCTCCA+AGG - chr4_4:23071213-23071232 Msa0674960:intron 55.0%
TTTGGGCCAACAGGGACTGT+GGG + chr4_4:23071239-23071258 None:intergenic 55.0%
ATTTGGGCCAACAGGGACTG+TGG + chr4_4:23071240-23071259 None:intergenic 55.0%
CAGTCCCTGTTGGCCCAAAT+TGG - chr4_4:23071240-23071259 Msa0674960:intron 55.0%
ATCTTGTGCCCTCAAGGCAC+AGG - chr4_4:23072583-23072602 Msa0674960:five_prime_UTR 55.0%
!! GTAGTGCTGGCTTGGGACCT+TGG + chr4_4:23070119-23070138 None:intergenic 60.0%
AGGTCCCAAGCCAGCACTAC+CGG - chr4_4:23070119-23070138 Msa0674960:intron 60.0%
!! TGGACCGGTAGTGCTGGCTT+GGG + chr4_4:23070126-23070145 None:intergenic 60.0%
!! CTTGGGTTAGCCTGGTGGAC+CGG + chr4_4:23070141-23070160 None:intergenic 60.0%
GTCCACCAGGCTAACCCAAG+TGG - chr4_4:23070141-23070160 Msa0674960:intron 60.0%
!! ACCCACTTGGGTTAGCCTGG+TGG + chr4_4:23070146-23070165 None:intergenic 60.0%
TGGCTGTGTAGGCCGGACTT+GGG + chr4_4:23071200-23071219 None:intergenic 60.0%
TTGGCTGTGTAGGCCGGACT+TGG + chr4_4:23071201-23071220 None:intergenic 60.0%
TGGGCCTTGGAGGTTGGGTT+TGG + chr4_4:23071220-23071239 None:intergenic 60.0%
GACTGTGGGCCTTGGAGGTT+GGG + chr4_4:23071225-23071244 None:intergenic 60.0%
!! GTGGACCGGTAGTGCTGGCT+TGG + chr4_4:23070127-23070146 None:intergenic 65.0%
GGACTGTGGGCCTTGGAGGT+TGG + chr4_4:23071226-23071245 None:intergenic 65.0%
ACAGGGACTGTGGGCCTTGG+AGG + chr4_4:23071230-23071249 None:intergenic 65.0%
CCAACAGGGACTGTGGGCCT+TGG + chr4_4:23071233-23071252 None:intergenic 65.0%
CCAAGGCCCACAGTCCCTGT+TGG - chr4_4:23071230-23071249 Msa0674960:intron 65.0%
GCCAGCACTACCGGTCCACC+AGG - chr4_4:23070128-23070147 Msa0674960:intron 70.0%
! GCCTGGTGGACCGGTAGTGC+TGG + chr4_4:23070132-23070151 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 23069397 23072646 23069397 ID=Msa0674960;Name=Msa0674960
chr4_4 mRNA 23069397 23072646 23069397 ID=Msa0674960-mRNA-1;Parent=Msa0674960;Name=Msa0674960-mRNA-1;_AED=0.02;_eAED=0.02;_QI=80|1|1|1|1|1|2|369|168
chr4_4 exon 23069397 23069911 23069397 ID=Msa0674960-mRNA-1:exon:6426;Parent=Msa0674960-mRNA-1
chr4_4 exon 23072206 23072646 23072206 ID=Msa0674960-mRNA-1:exon:6425;Parent=Msa0674960-mRNA-1
chr4_4 five_prime_UTR 23072567 23072646 23072567 ID=Msa0674960-mRNA-1:five_prime_utr;Parent=Msa0674960-mRNA-1
chr4_4 CDS 23072206 23072566 23072206 ID=Msa0674960-mRNA-1:cds;Parent=Msa0674960-mRNA-1
chr4_4 CDS 23069766 23069911 23069766 ID=Msa0674960-mRNA-1:cds;Parent=Msa0674960-mRNA-1
chr4_4 three_prime_UTR 23069397 23069765 23069397 ID=Msa0674960-mRNA-1:three_prime_utr;Parent=Msa0674960-mRNA-1
Gene Sequence

>Msa0674960

ATGGCGCTACAAACACAATTCGTAACTTCCTTCCCCACCAAACTACATCACTCATTCTCTCCTATCTTCACAATCAAGTCCGAAAAACCTTCTGAACCGTCCGATTCAGTTCCCAACAAAACTGGTGTGGGTTTTGGTCCGTCGAATGGCAACAACACCAAACGGAAACAGAAAGGGCAAAGAGAAAGAGCTTCGATAATTCGGCGTAATCCAGTTGAAAAACCGGCTTTCGTTTCGGAACAATTACAACAACAACAACAAGAAGTTCAAGAAGAGAGTGCTGTTGAGAAAGGTTTCATTCTTGCGTGGCTTGGTTTTGGTTCGGTTATTCTTGTTGAGGGTATTGCTCTTGCTGCTTCAGGCTTTTTTCCAGAAGAGTGGGACAATTTCCTTGTCAAGTATCTCTATCCATCCTTTACCCCTACAGTATTATTGTTTCTTGCTGGAGCAGTAGCATTTGGAGTGGTGAAATATAAGCAGAATGAGAAACTCACGGAGCGAAAATAG
Protein sequence

>Msa0674960

MALQTQFVTSFPTKLHHSFSPIFTIKSEKPSEPSDSVPNKTGVGFGPSNGNNTKRKQKGQRERASIIRRNPVEKPAFVSEQLQQQQQEVQEESAVEKGFILAWLGFGSVILVEGIALAASGFFPEEWDNFLVKYLYPSFTPTVLLFLAGAVAFGVVKYKQNEKLTERK*