AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0780450

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0780450 MtrunA17_Chr5g0399521 91.903 247 13 3 2 244 1 244 3.45e-149 416
Msa0780450 MtrunA17_Chr3g0126611 32.447 188 107 10 28 205 43 220 1.89e-15 74.7
Msa0780450 MtrunA17_Chr8g0347101 30.488 164 99 5 30 185 33 189 8.80e-15 72.8
Msa0780450 MtrunA17_Chr1g0158771 26.667 225 148 7 22 235 37 255 2.37e-12 66.2
Msa0780450 MtrunA17_Chr8g0388231 25.758 198 122 8 15 199 29 214 5.93e-11 61.6
Msa0780450 MtrunA17_Chr1g0160671 32.727 165 86 9 2 147 1 159 8.03e-11 60.8
Msa0780450 MtrunA17_Chr1g0160731 31.313 198 111 10 1 184 7 193 9.94e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0780450 AT5G53110.1 34.483 174 95 7 27 186 39 207 1.24e-16 78.6
Msa0780450 AT2G25410.1 28.395 243 137 10 31 242 42 278 1.61e-15 75.5
Msa0780450 AT2G46495.2 29.240 171 101 8 29 185 35 199 7.34e-14 70.5
Msa0780450 AT2G46495.1 29.240 171 101 8 29 185 35 199 7.80e-14 70.5
Msa0780450 AT5G36001.2 30.189 159 93 8 29 185 41 183 1.38e-12 65.5
Msa0780450 AT5G36001.1 30.189 159 93 8 29 185 41 183 4.88e-12 65.1

Find 61 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTTCTCACCCTCTTCT+TGG 0.138462 5_2:+62155585 Msa0780450:CDS
TATGATGATACCACCCTTTC+TGG 0.153018 5_2:+62155706 Msa0780450:CDS
AAGTTCTTCATTATCAAATT+TGG 0.203769 5_2:+62156836 Msa0780450:three_prime_UTR
TCACTATCCATTTCACATAA+AGG 0.295402 5_2:+62155660 Msa0780450:CDS
CAAGTAGACTTTGTGAAGTT+TGG 0.298271 5_2:-62155387 None:intergenic
CTTTGCATAAAGAATTAAAA+AGG 0.312037 5_2:-62155448 None:intergenic
TAAGGTTGAGAAAGACTCTT+TGG 0.318914 5_2:-62155852 None:intergenic
TGTCACACAAAGAAAATATA+TGG 0.321871 5_2:-62157047 None:intergenic
AGACTGTTGTCTGAAATATA+AGG 0.324770 5_2:-62156081 None:intergenic
CACTATCCATTTCACATAAA+GGG 0.339521 5_2:+62155661 Msa0780450:CDS
GAACTAGGCATAGGAACTTC+AGG 0.345941 5_2:-62156015 None:intergenic
CATAGCGTGGCAGATTTCTA+AGG 0.365268 5_2:+62156770 Msa0780450:CDS
AAGAAGAGTGAATTCATCAT+TGG 0.384353 5_2:-62155568 None:intergenic
CGTTGCAGTTAACAGAGGTT+TGG 0.396594 5_2:-62155618 None:intergenic
AACGCTTCAAGCGATGTTAG+AGG 0.407833 5_2:+62156916 Msa0780450:three_prime_UTR
TAAAATACGAACACTATTTG+AGG 0.411041 5_2:+62157113 Msa0780450:three_prime_UTR
TGTTGACCCTTTATGTGAAA+TGG 0.413795 5_2:-62155667 None:intergenic
GTCACGACTACATCAATCTA+AGG 0.414143 5_2:+62157081 Msa0780450:three_prime_UTR
GTTAACTGCAACGATATTGC+TGG 0.435527 5_2:+62155628 Msa0780450:CDS
GGCAACACTTGTAGGCATAA+AGG 0.435949 5_2:+62156712 Msa0780450:CDS
AGGACTTCTTGGTTTCTTGT+TGG 0.440422 5_2:-62155428 None:intergenic
GACTGTTGTCTGAAATATAA+GGG 0.447474 5_2:-62156080 None:intergenic
ACGGTGTGAGGGTGAGGTTA+AGG 0.448960 5_2:-62155870 None:intergenic
AACATCGCTTGAAGCGTTGA+GGG 0.457835 5_2:-62156911 None:intergenic
TATATTTCAGACAACAGTCT+TGG 0.476999 5_2:+62156084 Msa0780450:CDS
GCATAAAGGTTCATTTATCA+AGG 0.478351 5_2:+62156726 Msa0780450:CDS
GTAGACTTTGTGAAGTTTGG+TGG 0.479187 5_2:-62155384 None:intergenic
GTGGCACTGGCAACACTTGT+AGG 0.486667 5_2:+62156704 Msa0780450:CDS
AGAAGAGTGAATTCATCATT+GGG 0.494871 5_2:-62155567 None:intergenic
ACCATATGATGGAAAATGAA+TGG 0.499009 5_2:-62155761 None:intergenic
ACTATCAAATCACCATATGA+TGG 0.503242 5_2:-62155772 None:intergenic
ATTGGAACGGTGTGAGGGTG+AGG 0.507380 5_2:-62155876 None:intergenic
TGAAATGGATAGTGAATGTC+AGG 0.523321 5_2:-62155652 None:intergenic
AATCTGAAGAAAGCAAAGAA+TGG 0.524107 5_2:+62156133 Msa0780450:CDS
AGTGAATTCATCATTGGGAA+TGG 0.525024 5_2:-62155562 None:intergenic
AAGAAGCTCAAAACCAGAAA+GGG 0.525160 5_2:-62155719 None:intergenic
AACTAGGCATAGGAACTTCA+GGG 0.534743 5_2:-62156014 None:intergenic
CATTTATCAAGGAGGAAAGA+AGG 0.537861 5_2:+62156737 Msa0780450:CDS
CAAAATGACCAATTCTGTAA+AGG 0.539785 5_2:-62155361 None:intergenic
TCTCACACAGCACGCAATAC+AGG 0.540563 5_2:+62156171 Msa0780450:CDS
AGTAGTATTGGAACGGTGTG+AGG 0.573516 5_2:-62155882 None:intergenic
TAACATCGCTTGAAGCGTTG+AGG 0.577723 5_2:-62156912 None:intergenic
GAAGAAGCTCAAAACCAGAA+AGG 0.581187 5_2:-62155720 None:intergenic
GATTTCTAAGGACATAGTAA+AGG 0.589533 5_2:+62156782 Msa0780450:exon
ATATAAGGGCTATATTTGAA+AGG 0.590983 5_2:-62156066 None:intergenic
GAAGATGAAAACCAAGAAGA+GGG 0.591458 5_2:-62155596 None:intergenic
AGGACAATTGTTGCATAGCG+TGG 0.602254 5_2:+62156757 Msa0780450:CDS
CAAAGTACATATATTCTAGA+CGG 0.605467 5_2:+62155466 Msa0780450:five_prime_UTR
GGAAGATGAAAACCAAGAAG+AGG 0.611027 5_2:-62155597 None:intergenic
AGAACGTAGTAGTATTGGAA+CGG 0.613829 5_2:-62155889 None:intergenic
ATATTTGAAAGGAATAGCAA+TGG 0.622554 5_2:-62156055 None:intergenic
GGAATGGAGAAGATGTTATG+AGG 0.622795 5_2:-62155546 None:intergenic
TAACTTGAATCACTTAAACA+TGG 0.633744 5_2:-62155973 None:intergenic
GTAGTATTGGAACGGTGTGA+GGG 0.637754 5_2:-62155881 None:intergenic
GTCTTGGTCTTAGATTAACA+TGG 0.638466 5_2:+62156100 Msa0780450:CDS
AAAGTCTTGTGGAGCAATTG+AGG 0.638737 5_2:-62155927 None:intergenic
TAAAGGTTCATTTATCAAGG+AGG 0.641975 5_2:+62156729 Msa0780450:CDS
AATATCGTTGCAGTTAACAG+AGG 0.642706 5_2:-62155623 None:intergenic
TCTTGGTCTTAGATTAACAT+GGG 0.645510 5_2:+62156101 Msa0780450:CDS
AAGCTCAAAACCAGAAAGGG+TGG 0.646396 5_2:-62155716 None:intergenic
ATATGATGGAAAATGAATGG+CGG 0.781578 5_2:-62155758 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTTATTAACAAATTTGT+TGG + chr5_2:62156587-62156606 Msa0780450:intron 10.0%
!! AAATTTATTAACAAATTTGT+TGG + chr5_2:62156587-62156606 Msa0780450:intron 10.0%
!! AATTACAAAAATAAAGTTCA+TGG + chr5_2:62156219-62156238 Msa0780450:intron 15.0%
!! ATTACAAAAATAAAGTTCAT+GGG + chr5_2:62156220-62156239 Msa0780450:intron 15.0%
!! TGTATAGTACATATATATAT+TGG + chr5_2:62156355-62156374 Msa0780450:intron 15.0%
!! GTATAGTACATATATATATT+GGG + chr5_2:62156356-62156375 Msa0780450:intron 15.0%
!! AATTACAAAAATAAAGTTCA+TGG + chr5_2:62156219-62156238 Msa0780450:intron 15.0%
!! ATTACAAAAATAAAGTTCAT+GGG + chr5_2:62156220-62156239 Msa0780450:intron 15.0%
!! TGTATAGTACATATATATAT+TGG + chr5_2:62156355-62156374 Msa0780450:intron 15.0%
!! GTATAGTACATATATATATT+GGG + chr5_2:62156356-62156375 Msa0780450:intron 15.0%
!! CTTTGCATAAAGAATTAAAA+AGG - chr5_2:62155451-62155470 None:intergenic 20.0%
!!! TTTTACTTCTTATTCATTCT+TGG + chr5_2:62155499-62155518 Msa0780450:five_prime_UTR 20.0%
!! TTTGTAATTAAAGAAAGAGA+TGG - chr5_2:62156208-62156227 None:intergenic 20.0%
!! AAGTTCTTCATTATCAAATT+TGG + chr5_2:62156836-62156855 Msa0780450:three_prime_UTR 20.0%
!!! TTTTGTATAGTATATGTTGT+AGG + chr5_2:62156870-62156889 Msa0780450:three_prime_UTR 20.0%
!! CTTTGCATAAAGAATTAAAA+AGG - chr5_2:62155451-62155470 None:intergenic 20.0%
!!! TTTTACTTCTTATTCATTCT+TGG + chr5_2:62155499-62155518 Msa0780450:five_prime_UTR 20.0%
!! TTTGTAATTAAAGAAAGAGA+TGG - chr5_2:62156208-62156227 None:intergenic 20.0%
!! AAGTTCTTCATTATCAAATT+TGG + chr5_2:62156836-62156855 Msa0780450:three_prime_UTR 20.0%
!!! TTTTGTATAGTATATGTTGT+AGG + chr5_2:62156870-62156889 Msa0780450:three_prime_UTR 20.0%
! AAGAATTAAAAAGGACTTCT+TGG - chr5_2:62155442-62155461 None:intergenic 25.0%
! CAAAGTACATATATTCTAGA+CGG + chr5_2:62155466-62155485 Msa0780450:five_prime_UTR 25.0%
!! ATCAAGAAGATCAATCTTTT+TGG - chr5_2:62155818-62155837 None:intergenic 25.0%
!!! TTTTAAGAACGTAGTAGTAT+TGG - chr5_2:62155897-62155916 None:intergenic 25.0%
! TAACTTGAATCACTTAAACA+TGG - chr5_2:62155976-62155995 None:intergenic 25.0%
!!! TTGACTTTTTTACATGAACT+AGG - chr5_2:62156033-62156052 None:intergenic 25.0%
! ATATTTGAAAGGAATAGCAA+TGG - chr5_2:62156058-62156077 None:intergenic 25.0%
! ATATAAGGGCTATATTTGAA+AGG - chr5_2:62156069-62156088 None:intergenic 25.0%
! AGGAAATTATGCTCATTTAA+AGG - chr5_2:62156264-62156283 None:intergenic 25.0%
! ATAATTTCCTTATACTAGGT+TGG + chr5_2:62156274-62156293 Msa0780450:intron 25.0%
! TAATTTCCTTATACTAGGTT+GGG + chr5_2:62156275-62156294 Msa0780450:intron 25.0%
! AGTACATATATATATTGGGA+AGG + chr5_2:62156360-62156379 Msa0780450:intron 25.0%
! ATCATTACGAAGTATATACT+AGG + chr5_2:62156414-62156433 Msa0780450:intron 25.0%
! TGTCACACAAAGAAAATATA+TGG - chr5_2:62157050-62157069 None:intergenic 25.0%
! TAAAATACGAACACTATTTG+AGG + chr5_2:62157113-62157132 Msa0780450:three_prime_UTR 25.0%
! AAGAATTAAAAAGGACTTCT+TGG - chr5_2:62155442-62155461 None:intergenic 25.0%
! CAAAGTACATATATTCTAGA+CGG + chr5_2:62155466-62155485 Msa0780450:five_prime_UTR 25.0%
!! ATCAAGAAGATCAATCTTTT+TGG - chr5_2:62155818-62155837 None:intergenic 25.0%
!!! TTTTAAGAACGTAGTAGTAT+TGG - chr5_2:62155897-62155916 None:intergenic 25.0%
! TAACTTGAATCACTTAAACA+TGG - chr5_2:62155976-62155995 None:intergenic 25.0%
!!! TTGACTTTTTTACATGAACT+AGG - chr5_2:62156033-62156052 None:intergenic 25.0%
! ATATTTGAAAGGAATAGCAA+TGG - chr5_2:62156058-62156077 None:intergenic 25.0%
! ATATAAGGGCTATATTTGAA+AGG - chr5_2:62156069-62156088 None:intergenic 25.0%
! AGGAAATTATGCTCATTTAA+AGG - chr5_2:62156264-62156283 None:intergenic 25.0%
! ATAATTTCCTTATACTAGGT+TGG + chr5_2:62156274-62156293 Msa0780450:intron 25.0%
! TAATTTCCTTATACTAGGTT+GGG + chr5_2:62156275-62156294 Msa0780450:intron 25.0%
! AGTACATATATATATTGGGA+AGG + chr5_2:62156360-62156379 Msa0780450:intron 25.0%
! ATCATTACGAAGTATATACT+AGG + chr5_2:62156414-62156433 Msa0780450:intron 25.0%
! TGTCACACAAAGAAAATATA+TGG - chr5_2:62157050-62157069 None:intergenic 25.0%
! TAAAATACGAACACTATTTG+AGG + chr5_2:62157113-62157132 Msa0780450:three_prime_UTR 25.0%
! TTTTTGCTCCTTTACAGAAT+TGG + chr5_2:62155353-62155372 Msa0780450:five_prime_UTR 30.0%
CAAAATGACCAATTCTGTAA+AGG - chr5_2:62155364-62155383 None:intergenic 30.0%
AGAAGAGTGAATTCATCATT+GGG - chr5_2:62155570-62155589 None:intergenic 30.0%
AAGAAGAGTGAATTCATCAT+TGG - chr5_2:62155571-62155590 None:intergenic 30.0%
TCACTATCCATTTCACATAA+AGG + chr5_2:62155660-62155679 Msa0780450:CDS 30.0%
CACTATCCATTTCACATAAA+GGG + chr5_2:62155661-62155680 Msa0780450:CDS 30.0%
! ATATGATGGAAAATGAATGG+CGG - chr5_2:62155761-62155780 None:intergenic 30.0%
! ACCATATGATGGAAAATGAA+TGG - chr5_2:62155764-62155783 None:intergenic 30.0%
ACTATCAAATCACCATATGA+TGG - chr5_2:62155775-62155794 None:intergenic 30.0%
!!! ATCAATCTTTTTGGTGTCAT+AGG - chr5_2:62155809-62155828 None:intergenic 30.0%
!! TTTTGTTTGTGAAAGTCTTG+TGG - chr5_2:62155941-62155960 None:intergenic 30.0%
! TTTTTACATGAACTAGGCAT+AGG - chr5_2:62156027-62156046 None:intergenic 30.0%
! GACTGTTGTCTGAAATATAA+GGG - chr5_2:62156083-62156102 None:intergenic 30.0%
! AGACTGTTGTCTGAAATATA+AGG - chr5_2:62156084-62156103 None:intergenic 30.0%
TATATTTCAGACAACAGTCT+TGG + chr5_2:62156084-62156103 Msa0780450:CDS 30.0%
!! TCTTGGTCTTAGATTAACAT+GGG + chr5_2:62156101-62156120 Msa0780450:CDS 30.0%
AATCTGAAGAAAGCAAAGAA+TGG + chr5_2:62156133-62156152 Msa0780450:CDS 30.0%
GAGCATAATTTCCTTATACT+AGG + chr5_2:62156270-62156289 Msa0780450:intron 30.0%
TTACGAAGTATATACTAGGT+TGG + chr5_2:62156418-62156437 Msa0780450:intron 30.0%
TACGAAGTATATACTAGGTT+GGG + chr5_2:62156419-62156438 Msa0780450:intron 30.0%
TAGTACATTATCTTTCGTGT+AGG + chr5_2:62156483-62156502 Msa0780450:intron 30.0%
GCATAAAGGTTCATTTATCA+AGG + chr5_2:62156726-62156745 Msa0780450:CDS 30.0%
TAAAGGTTCATTTATCAAGG+AGG + chr5_2:62156729-62156748 Msa0780450:CDS 30.0%
! GATTTCTAAGGACATAGTAA+AGG + chr5_2:62156782-62156801 Msa0780450:exon 30.0%
AGTGTCTAAACTTCAATGTT+GGG - chr5_2:62155299-62155318 None:intergenic 30.0%
AAGTGTCTAAACTTCAATGT+TGG - chr5_2:62155300-62155319 None:intergenic 30.0%
! TTTTTGCTCCTTTACAGAAT+TGG + chr5_2:62155353-62155372 Msa0780450:five_prime_UTR 30.0%
CAAAATGACCAATTCTGTAA+AGG - chr5_2:62155364-62155383 None:intergenic 30.0%
AGAAGAGTGAATTCATCATT+GGG - chr5_2:62155570-62155589 None:intergenic 30.0%
AAGAAGAGTGAATTCATCAT+TGG - chr5_2:62155571-62155590 None:intergenic 30.0%
TCACTATCCATTTCACATAA+AGG + chr5_2:62155660-62155679 Msa0780450:CDS 30.0%
CACTATCCATTTCACATAAA+GGG + chr5_2:62155661-62155680 Msa0780450:CDS 30.0%
! ATATGATGGAAAATGAATGG+CGG - chr5_2:62155761-62155780 None:intergenic 30.0%
! ACCATATGATGGAAAATGAA+TGG - chr5_2:62155764-62155783 None:intergenic 30.0%
ACTATCAAATCACCATATGA+TGG - chr5_2:62155775-62155794 None:intergenic 30.0%
!!! ATCAATCTTTTTGGTGTCAT+AGG - chr5_2:62155809-62155828 None:intergenic 30.0%
!! TTTTGTTTGTGAAAGTCTTG+TGG - chr5_2:62155941-62155960 None:intergenic 30.0%
! TTTTTACATGAACTAGGCAT+AGG - chr5_2:62156027-62156046 None:intergenic 30.0%
! GACTGTTGTCTGAAATATAA+GGG - chr5_2:62156083-62156102 None:intergenic 30.0%
! AGACTGTTGTCTGAAATATA+AGG - chr5_2:62156084-62156103 None:intergenic 30.0%
TATATTTCAGACAACAGTCT+TGG + chr5_2:62156084-62156103 Msa0780450:CDS 30.0%
!! TCTTGGTCTTAGATTAACAT+GGG + chr5_2:62156101-62156120 Msa0780450:CDS 30.0%
AATCTGAAGAAAGCAAAGAA+TGG + chr5_2:62156133-62156152 Msa0780450:CDS 30.0%
GAGCATAATTTCCTTATACT+AGG + chr5_2:62156270-62156289 Msa0780450:intron 30.0%
TTACGAAGTATATACTAGGT+TGG + chr5_2:62156418-62156437 Msa0780450:intron 30.0%
TACGAAGTATATACTAGGTT+GGG + chr5_2:62156419-62156438 Msa0780450:intron 30.0%
TAGTACATTATCTTTCGTGT+AGG + chr5_2:62156483-62156502 Msa0780450:intron 30.0%
GCATAAAGGTTCATTTATCA+AGG + chr5_2:62156726-62156745 Msa0780450:CDS 30.0%
TAAAGGTTCATTTATCAAGG+AGG + chr5_2:62156729-62156748 Msa0780450:CDS 30.0%
! GATTTCTAAGGACATAGTAA+AGG + chr5_2:62156782-62156801 Msa0780450:exon 30.0%
CAAGTAGACTTTGTGAAGTT+TGG - chr5_2:62155390-62155409 None:intergenic 35.0%
AGTGAATTCATCATTGGGAA+TGG - chr5_2:62155565-62155584 None:intergenic 35.0%
GAAGATGAAAACCAAGAAGA+GGG - chr5_2:62155599-62155618 None:intergenic 35.0%
AATATCGTTGCAGTTAACAG+AGG - chr5_2:62155626-62155645 None:intergenic 35.0%
TGAAATGGATAGTGAATGTC+AGG - chr5_2:62155655-62155674 None:intergenic 35.0%
TGTTGACCCTTTATGTGAAA+TGG - chr5_2:62155670-62155689 None:intergenic 35.0%
AAGAAGCTCAAAACCAGAAA+GGG - chr5_2:62155722-62155741 None:intergenic 35.0%
! GCCATTCATTTTCCATCATA+TGG + chr5_2:62155760-62155779 Msa0780450:CDS 35.0%
!!! CTTTTTGGTGTCATAGGAAA+TGG - chr5_2:62155803-62155822 None:intergenic 35.0%
! ACTCTTTGGACACAGTTTTT+GGG - chr5_2:62155841-62155860 None:intergenic 35.0%
TAAGGTTGAGAAAGACTCTT+TGG - chr5_2:62155855-62155874 None:intergenic 35.0%
! AGAACGTAGTAGTATTGGAA+CGG - chr5_2:62155892-62155911 None:intergenic 35.0%
!! GTCTTGGTCTTAGATTAACA+TGG + chr5_2:62156100-62156119 Msa0780450:CDS 35.0%
AAACTACCCAACCTAGTATA+AGG - chr5_2:62156284-62156303 None:intergenic 35.0%
! ATATACTAGGTTGGGTAGTT+TGG + chr5_2:62156427-62156446 Msa0780450:intron 35.0%
TCTTTCGTGTAGGATTGATT+CGG + chr5_2:62156493-62156512 Msa0780450:intron 35.0%
CATTTATCAAGGAGGAAAGA+AGG + chr5_2:62156737-62156756 Msa0780450:CDS 35.0%
GTGTCTAAACTTCAATGTTG+GGG - chr5_2:62155298-62155317 None:intergenic 35.0%
CAAGTAGACTTTGTGAAGTT+TGG - chr5_2:62155390-62155409 None:intergenic 35.0%
AGTGAATTCATCATTGGGAA+TGG - chr5_2:62155565-62155584 None:intergenic 35.0%
GAAGATGAAAACCAAGAAGA+GGG - chr5_2:62155599-62155618 None:intergenic 35.0%
AATATCGTTGCAGTTAACAG+AGG - chr5_2:62155626-62155645 None:intergenic 35.0%
TGAAATGGATAGTGAATGTC+AGG - chr5_2:62155655-62155674 None:intergenic 35.0%
TGTTGACCCTTTATGTGAAA+TGG - chr5_2:62155670-62155689 None:intergenic 35.0%
AAGAAGCTCAAAACCAGAAA+GGG - chr5_2:62155722-62155741 None:intergenic 35.0%
! GCCATTCATTTTCCATCATA+TGG + chr5_2:62155760-62155779 Msa0780450:CDS 35.0%
!!! CTTTTTGGTGTCATAGGAAA+TGG - chr5_2:62155803-62155822 None:intergenic 35.0%
! ACTCTTTGGACACAGTTTTT+GGG - chr5_2:62155841-62155860 None:intergenic 35.0%
TAAGGTTGAGAAAGACTCTT+TGG - chr5_2:62155855-62155874 None:intergenic 35.0%
! AGAACGTAGTAGTATTGGAA+CGG - chr5_2:62155892-62155911 None:intergenic 35.0%
!! GTCTTGGTCTTAGATTAACA+TGG + chr5_2:62156100-62156119 Msa0780450:CDS 35.0%
AAACTACCCAACCTAGTATA+AGG - chr5_2:62156284-62156303 None:intergenic 35.0%
! ATATACTAGGTTGGGTAGTT+TGG + chr5_2:62156427-62156446 Msa0780450:intron 35.0%
TCTTTCGTGTAGGATTGATT+CGG + chr5_2:62156493-62156512 Msa0780450:intron 35.0%
CATTTATCAAGGAGGAAAGA+AGG + chr5_2:62156737-62156756 Msa0780450:CDS 35.0%
GTAGACTTTGTGAAGTTTGG+TGG - chr5_2:62155387-62155406 None:intergenic 40.0%
!! TGTTGGCTTCAAAAAGCTCA+GGG - chr5_2:62155414-62155433 None:intergenic 40.0%
!! TTGTTGGCTTCAAAAAGCTC+AGG - chr5_2:62155415-62155434 None:intergenic 40.0%
AGGACTTCTTGGTTTCTTGT+TGG - chr5_2:62155431-62155450 None:intergenic 40.0%
GGAATGGAGAAGATGTTATG+AGG - chr5_2:62155549-62155568 None:intergenic 40.0%
GGAAGATGAAAACCAAGAAG+AGG - chr5_2:62155600-62155619 None:intergenic 40.0%
GTTAACTGCAACGATATTGC+TGG + chr5_2:62155628-62155647 Msa0780450:CDS 40.0%
TATGATGATACCACCCTTTC+TGG + chr5_2:62155706-62155725 Msa0780450:CDS 40.0%
GAAGAAGCTCAAAACCAGAA+AGG - chr5_2:62155723-62155742 None:intergenic 40.0%
! GACTCTTTGGACACAGTTTT+TGG - chr5_2:62155842-62155861 None:intergenic 40.0%
! AAAGTCTTGTGGAGCAATTG+AGG - chr5_2:62155930-62155949 None:intergenic 40.0%
AACTAGGCATAGGAACTTCA+GGG - chr5_2:62156017-62156036 None:intergenic 40.0%
TTCAACACATGTGCAGTTCT+TGG + chr5_2:62156659-62156678 Msa0780450:intron 40.0%
! TCTCAGCATCTGTATTTTCG+TGG + chr5_2:62156682-62156701 Msa0780450:CDS 40.0%
GTCACGACTACATCAATCTA+AGG + chr5_2:62157081-62157100 Msa0780450:three_prime_UTR 40.0%
GTAGACTTTGTGAAGTTTGG+TGG - chr5_2:62155387-62155406 None:intergenic 40.0%
!! TGTTGGCTTCAAAAAGCTCA+GGG - chr5_2:62155414-62155433 None:intergenic 40.0%
!! TTGTTGGCTTCAAAAAGCTC+AGG - chr5_2:62155415-62155434 None:intergenic 40.0%
AGGACTTCTTGGTTTCTTGT+TGG - chr5_2:62155431-62155450 None:intergenic 40.0%
GGAATGGAGAAGATGTTATG+AGG - chr5_2:62155549-62155568 None:intergenic 40.0%
GGAAGATGAAAACCAAGAAG+AGG - chr5_2:62155600-62155619 None:intergenic 40.0%
GTTAACTGCAACGATATTGC+TGG + chr5_2:62155628-62155647 Msa0780450:CDS 40.0%
TATGATGATACCACCCTTTC+TGG + chr5_2:62155706-62155725 Msa0780450:CDS 40.0%
GAAGAAGCTCAAAACCAGAA+AGG - chr5_2:62155723-62155742 None:intergenic 40.0%
! GACTCTTTGGACACAGTTTT+TGG - chr5_2:62155842-62155861 None:intergenic 40.0%
! AAAGTCTTGTGGAGCAATTG+AGG - chr5_2:62155930-62155949 None:intergenic 40.0%
AACTAGGCATAGGAACTTCA+GGG - chr5_2:62156017-62156036 None:intergenic 40.0%
TTCAACACATGTGCAGTTCT+TGG + chr5_2:62156659-62156678 Msa0780450:intron 40.0%
! TCTCAGCATCTGTATTTTCG+TGG + chr5_2:62156682-62156701 Msa0780450:CDS 40.0%
GTCACGACTACATCAATCTA+AGG + chr5_2:62157081-62157100 Msa0780450:three_prime_UTR 40.0%
CTTCTTTCTCACCCTCTTCT+TGG + chr5_2:62155585-62155604 Msa0780450:CDS 45.0%
CGTTGCAGTTAACAGAGGTT+TGG - chr5_2:62155621-62155640 None:intergenic 45.0%
AAGCTCAAAACCAGAAAGGG+TGG - chr5_2:62155719-62155738 None:intergenic 45.0%
! GTAGTATTGGAACGGTGTGA+GGG - chr5_2:62155884-62155903 None:intergenic 45.0%
! AGTAGTATTGGAACGGTGTG+AGG - chr5_2:62155885-62155904 None:intergenic 45.0%
GAACTAGGCATAGGAACTTC+AGG - chr5_2:62156018-62156037 None:intergenic 45.0%
! CAGCATCTGTATTTTCGTGG+TGG + chr5_2:62156685-62156704 Msa0780450:CDS 45.0%
GGCAACACTTGTAGGCATAA+AGG + chr5_2:62156712-62156731 Msa0780450:CDS 45.0%
AGGACAATTGTTGCATAGCG+TGG + chr5_2:62156757-62156776 Msa0780450:CDS 45.0%
CATAGCGTGGCAGATTTCTA+AGG + chr5_2:62156770-62156789 Msa0780450:CDS 45.0%
!! AACATCGCTTGAAGCGTTGA+GGG - chr5_2:62156914-62156933 None:intergenic 45.0%
!! TAACATCGCTTGAAGCGTTG+AGG - chr5_2:62156915-62156934 None:intergenic 45.0%
AACGCTTCAAGCGATGTTAG+AGG + chr5_2:62156916-62156935 Msa0780450:three_prime_UTR 45.0%
CTTCTTTCTCACCCTCTTCT+TGG + chr5_2:62155585-62155604 Msa0780450:CDS 45.0%
CGTTGCAGTTAACAGAGGTT+TGG - chr5_2:62155621-62155640 None:intergenic 45.0%
AAGCTCAAAACCAGAAAGGG+TGG - chr5_2:62155719-62155738 None:intergenic 45.0%
! GTAGTATTGGAACGGTGTGA+GGG - chr5_2:62155884-62155903 None:intergenic 45.0%
! AGTAGTATTGGAACGGTGTG+AGG - chr5_2:62155885-62155904 None:intergenic 45.0%
GAACTAGGCATAGGAACTTC+AGG - chr5_2:62156018-62156037 None:intergenic 45.0%
! CAGCATCTGTATTTTCGTGG+TGG + chr5_2:62156685-62156704 Msa0780450:CDS 45.0%
GGCAACACTTGTAGGCATAA+AGG + chr5_2:62156712-62156731 Msa0780450:CDS 45.0%
AGGACAATTGTTGCATAGCG+TGG + chr5_2:62156757-62156776 Msa0780450:CDS 45.0%
CATAGCGTGGCAGATTTCTA+AGG + chr5_2:62156770-62156789 Msa0780450:CDS 45.0%
!! AACATCGCTTGAAGCGTTGA+GGG - chr5_2:62156914-62156933 None:intergenic 45.0%
!! TAACATCGCTTGAAGCGTTG+AGG - chr5_2:62156915-62156934 None:intergenic 45.0%
AACGCTTCAAGCGATGTTAG+AGG + chr5_2:62156916-62156935 Msa0780450:three_prime_UTR 45.0%
TCTCACACAGCACGCAATAC+AGG + chr5_2:62156171-62156190 Msa0780450:CDS 50.0%
! CTGTATTTTCGTGGTGGCAC+TGG + chr5_2:62156691-62156710 Msa0780450:CDS 50.0%
TCTCACACAGCACGCAATAC+AGG + chr5_2:62156171-62156190 Msa0780450:CDS 50.0%
! CTGTATTTTCGTGGTGGCAC+TGG + chr5_2:62156691-62156710 Msa0780450:CDS 50.0%
ACGGTGTGAGGGTGAGGTTA+AGG - chr5_2:62155873-62155892 None:intergenic 55.0%
ATTGGAACGGTGTGAGGGTG+AGG - chr5_2:62155879-62155898 None:intergenic 55.0%
!! GTGGCACTGGCAACACTTGT+AGG + chr5_2:62156704-62156723 Msa0780450:CDS 55.0%
ACGGTGTGAGGGTGAGGTTA+AGG - chr5_2:62155873-62155892 None:intergenic 55.0%
ATTGGAACGGTGTGAGGGTG+AGG - chr5_2:62155879-62155898 None:intergenic 55.0%
!! GTGGCACTGGCAACACTTGT+AGG + chr5_2:62156704-62156723 Msa0780450:CDS 55.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 62155298 62157169 62155298 ID=Msa0780450;Name=Msa0780450
chr5_2 mRNA 62155298 62157169 62155298 ID=Msa0780450-mRNA-1;Parent=Msa0780450;Name=Msa0780450-mRNA-1;_AED=0.01;_eAED=0.01;_QI=273|1|1|1|1|1|2|379|245
chr5_2 exon 62155298 62156192 62155298 ID=Msa0780450-mRNA-1:exon:18373;Parent=Msa0780450-mRNA-1
chr5_2 exon 62156675 62157169 62156675 ID=Msa0780450-mRNA-1:exon:18374;Parent=Msa0780450-mRNA-1
chr5_2 five_prime_UTR 62155298 62155570 62155298 ID=Msa0780450-mRNA-1:five_prime_utr;Parent=Msa0780450-mRNA-1
chr5_2 CDS 62155571 62156192 62155571 ID=Msa0780450-mRNA-1:cds;Parent=Msa0780450-mRNA-1
chr5_2 CDS 62156675 62156790 62156675 ID=Msa0780450-mRNA-1:cds;Parent=Msa0780450-mRNA-1
chr5_2 three_prime_UTR 62156791 62157169 62156791 ID=Msa0780450-mRNA-1:three_prime_utr;Parent=Msa0780450-mRNA-1
Gene Sequence

>Msa0780450

ATGATGAATTCACTCTTCTTTCTCACCCTCTTCTTGGTTTTCATCTTCCAAACCTCTGTTAACTGCAACGATATTGCTGGTCCTGACATTCACTATCCATTTCACATAAAGGGTCAACAGCAACAACAACAAGAGTATGATGATACCACCCTTTCTGGTTTTGAGCTTCTTCGCAAAGACAACATCACCGCCATTCATTTTCCATCATATGGTGATTTGATAGTCAAGTCCATTTCCTATGACACCAAAAAGATTGATCTTCTTGATCCCAAAAACTGTGTCCAAAGAGTCTTTCTCAACCTTAACCTCACCCTCACACCGTTCCAATACTACTACGTTCTTAAAAACTTCACATACCTCAATTGCTCCACAAGACTTTCACAAACAAAATTCATTGAAGTTCCATGTTTAAGTGATTCAAGTTATCATGTTTACACAGTTGACCCTGAAGTTCCTATGCCTAGTTCATGTAAAAAAGTCAAAACCATTGCTATTCCTTTCAAATATAGCCCTTATATTTCAGACAACAGTCTTGGTCTTAGATTAACATGGGACTTGCGTGAATCTGAAGAAAGCAAAGAATGGAACAAGACTAGAGATTCTCACACAGCACGCAATACAGTTCTTGGTCTCAGCATCTGTATTTTCGTGGTGGCACTGGCAACACTTGTAGGCATAAAGGTTCATTTATCAAGGAGGAAAGAAGGACAATTGTTGCATAGCGTGGCAGATTTCTAA
Protein sequence

>Msa0780450

MMNSLFFLTLFLVFIFQTSVNCNDIAGPDIHYPFHIKGQQQQQQEYDDTTLSGFELLRKDNITAIHFPSYGDLIVKSISYDTKKIDLLDPKNCVQRVFLNLNLTLTPFQYYYVLKNFTYLNCSTRLSQTKFIEVPCLSDSSYHVYTVDPEVPMPSSCKKVKTIAIPFKYSPYISDNSLGLRLTWDLRESEESKEWNKTRDSHTARNTVLGLSICIFVVALATLVGIKVHLSRRKEGQLLHSVADF*