AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0786230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0786230 MtrunA17_Chr5g0443431 98.726 157 2 0 1 157 1 157 5.51e-116 324
Msa0786230 MtrunA17_Chr3g0097141 79.114 158 32 1 1 157 1 158 2.23e-94 270
Msa0786230 MtrunA17_Chr4g0060581 51.333 150 71 1 4 153 12 159 1.25e-46 149
Msa0786230 MtrunA17_Chr5g0416561 44.578 166 74 2 2 155 64 223 1.39e-40 136
Msa0786230 MtrunA17_Chr3g0132031 48.760 121 56 1 1 121 1 115 7.23e-34 116
Msa0786230 MtrunA17_Chr8g0386221 50.962 104 51 0 1 104 1 104 5.91e-33 112
Msa0786230 MtrunA17_Chr4g0049651 33.088 136 77 2 17 147 20 146 2.34e-20 86.7
Msa0786230 MtrunA17_Chr4g0049701 31.159 138 81 2 17 149 17 145 9.12e-20 84.0
Msa0786230 MtrunA17_Chr4g0049671 32.540 126 71 2 27 147 21 137 4.73e-17 77.0
Msa0786230 MtrunA17_Chr5g0403731 29.688 128 78 2 7 134 28 143 2.78e-14 67.0
Msa0786230 MtrunA17_Chr5g0427761 34.524 84 55 0 17 100 22 105 5.81e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0786230 AT2G42820.1 63.291 158 57 1 1 157 1 158 2.47e-74 219
Msa0786230 AT1G74520.1 46.053 152 80 1 4 155 9 158 4.24e-42 138
Msa0786230 AT1G74520.2 46.053 152 80 1 4 155 9 158 4.24e-42 138
Msa0786230 AT1G69700.1 44.966 149 79 1 2 147 9 157 4.88e-37 125
Msa0786230 AT5G50720.1 58.095 105 44 0 1 105 1 105 2.23e-35 119
Msa0786230 AT5G62490.1 56.842 95 41 0 7 101 12 106 1.40e-34 119
Msa0786230 AT4G24960.1 45.669 127 68 1 8 134 8 133 4.15e-32 112
Msa0786230 AT4G24960.3 40.441 136 71 1 20 155 1 126 1.31e-29 105
Msa0786230 AT4G24960.2 41.748 103 59 1 32 134 1 102 7.63e-23 87.4
Msa0786230 AT4G36720.1 36.842 95 56 1 7 101 36 126 5.81e-16 72.0

Find 45 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGAGAGATTAATGAAGTTT+TGG 0.269958 5_2:+71640919 None:intergenic
AGGACATACGTCAAGAAATT+TGG 0.280571 5_2:-71640044 Msa0786230:CDS
GTCAAGAAATTTGGTAACTA+TGG 0.297751 5_2:-71640035 Msa0786230:CDS
ACCAACAGTGGCTAACATAT+TGG 0.311228 5_2:-71640314 Msa0786230:CDS
GAACGTTATATTGATAGATT+TGG 0.323087 5_2:-71639942 Msa0786230:CDS
CCAACAGTGGCTAACATATT+GGG 0.341811 5_2:-71640313 Msa0786230:CDS
CAACTTACAAGGTCCTATAT+TGG 0.344124 5_2:-71640257 Msa0786230:intron
ATCACTCTCTCAAATGCATC+TGG 0.360770 5_2:+71639918 None:intergenic
ATATCAATAGGTTTCCCATT+TGG 0.373951 5_2:-71640148 Msa0786230:intron
AGACATTTGGACACAACAAT+AGG 0.378599 5_2:-71640857 Msa0786230:CDS
ATTGCTGAACCTACCAATAT+AGG 0.380907 5_2:+71640244 None:intergenic
AAGCTTCATGTATGGCCAAA+TGG 0.383376 5_2:+71640133 None:intergenic
TGGAGCAATACTAAGACATT+TGG 0.388072 5_2:-71640870 Msa0786230:CDS
AGAGTTGGAGCAAAAGATTA+TGG 0.394037 5_2:+71640944 None:intergenic
CTTCGAGCTTTCAACTTACA+AGG 0.426910 5_2:-71640268 Msa0786230:CDS
AAAATCATCATGGGTGTCCT+TGG 0.439409 5_2:-71640890 Msa0786230:exon
AGCTTCATGTATGGCCAAAT+GGG 0.440653 5_2:+71640134 None:intergenic
TTCTTCTTCCCATGCCAGCT+TGG 0.474007 5_2:+71639738 None:intergenic
GACATTTGGACACAACAATA+GGG 0.477953 5_2:-71640856 Msa0786230:intron
TCAATTTAACCAAGCTGGCA+TGG 0.493645 5_2:-71639747 Msa0786230:three_prime_UTR
TATTGAAAGTCCTTCAACCT+TGG 0.507265 5_2:-71640340 Msa0786230:CDS
TGAAGCTTGTGTTCTGCGTA+TGG 0.521277 5_2:-71640118 Msa0786230:CDS
TGTTGGTCATCCAAGGTTGA+AGG 0.521506 5_2:+71640330 None:intergenic
AGAACCTTCTTGTACTCCTC+AGG 0.524082 5_2:+71639999 None:intergenic
CCCAATATGTTAGCCACTGT+TGG 0.527231 5_2:+71640313 None:intergenic
ATGGAATCAATGTTGTATTG+TGG 0.528844 5_2:+71640963 None:intergenic
TTTATTCTTAATTAATCCAA+GGG 0.530381 5_2:+71639571 None:intergenic
CAATTTAACCAAGCTGGCAT+GGG 0.541105 5_2:-71639746 Msa0786230:three_prime_UTR
CAGAACACAAGCTTCATGTA+TGG 0.542978 5_2:+71640125 None:intergenic
TAGCCACTGTTGGTCATCCA+AGG 0.555283 5_2:+71640323 None:intergenic
AGGATAAAGAAGCATTACTC+CGG 0.560010 5_2:+71640468 None:intergenic
TTGGTACTGCCAATGTTCAA+TGG 0.561469 5_2:-71640095 Msa0786230:CDS
GCTTGTGTTCTGCGTATGGT+TGG 0.576715 5_2:-71640114 Msa0786230:CDS
GGATAAAGAAGCATTACTCC+GGG 0.584585 5_2:+71640469 None:intergenic
GAGCGTCAATTTAACCAAGC+TGG 0.586351 5_2:-71639752 Msa0786230:three_prime_UTR
TCATAGATACGCATCAATGA+GGG 0.589923 5_2:-71640364 Msa0786230:intron
ATCATAGATACGCATCAATG+AGG 0.601236 5_2:-71640365 Msa0786230:intron
TCACTCTCTCAAATGCATCT+GGG 0.601880 5_2:+71639919 None:intergenic
ACTTCATTAATCTCTCCACC+TGG 0.615826 5_2:-71640914 Msa0786230:five_prime_UTR
TAAGCTGCTCCATTGAACAT+TGG 0.632735 5_2:+71640086 None:intergenic
TTATCCTGAGGAGTACAAGA+AGG 0.638050 5_2:-71640003 Msa0786230:CDS
AATGTCTTAGTATTGCTCCA+AGG 0.660944 5_2:+71640873 None:intergenic
ACATATATGAGAATTATGCA+AGG 0.691799 5_2:-71640064 Msa0786230:CDS
CAACCTTGGATGACCAACAG+TGG 0.691907 5_2:-71640326 Msa0786230:CDS
TGGAAATTACAATTATCCTG+AGG 0.710511 5_2:-71640015 Msa0786230:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCTATAATTATCTTTTTAT+TGG - chr5_2:71639809-71639828 Msa0786230:intron 10.0%
!!! TTCTATAATTATCTTTTTAT+TGG - chr5_2:71639809-71639828 Msa0786230:intron 10.0%
!! AAAAGATAATTATAGAAAAG+TGG + chr5_2:71639806-71639825 None:intergenic 15.0%
!!! TATAATTATCTTTTTATTGG+TGG - chr5_2:71639812-71639831 Msa0786230:intron 15.0%
!!! ACAATTTGATAATATTTATC+TGG - chr5_2:71640353-71640372 Msa0786230:CDS 15.0%
!! GCTTAATAAAAAATATCAAT+AGG - chr5_2:71640375-71640394 Msa0786230:intron 15.0%
!! TAAAGATATATTTGTAACTT+TGG + chr5_2:71640673-71640692 None:intergenic 15.0%
!! TTTATTCTTAATTAATCCAA+GGG + chr5_2:71640967-71640986 None:intergenic 15.0%
!!! TTTTATTCTTAATTAATCCA+AGG + chr5_2:71640968-71640987 None:intergenic 15.0%
!! AAAAGATAATTATAGAAAAG+TGG + chr5_2:71639806-71639825 None:intergenic 15.0%
!!! TATAATTATCTTTTTATTGG+TGG - chr5_2:71639812-71639831 Msa0786230:intron 15.0%
!!! ACAATTTGATAATATTTATC+TGG - chr5_2:71640353-71640372 Msa0786230:CDS 15.0%
!! GCTTAATAAAAAATATCAAT+AGG - chr5_2:71640375-71640394 Msa0786230:intron 15.0%
!! TAAAGATATATTTGTAACTT+TGG + chr5_2:71640673-71640692 None:intergenic 15.0%
!! TTTATTCTTAATTAATCCAA+GGG + chr5_2:71640967-71640986 None:intergenic 15.0%
!!! TTTTATTCTTAATTAATCCA+AGG + chr5_2:71640968-71640987 None:intergenic 15.0%
!! TTCTATAATTATCTAGATGA+AGG - chr5_2:71639911-71639930 Msa0786230:CDS 20.0%
!! AATATGATCATTACTTACAA+AGG + chr5_2:71640090-71640109 None:intergenic 20.0%
!! TCTATTTCACTTTATATTTC+AGG - chr5_2:71640732-71640751 Msa0786230:intron 20.0%
!! TTCTATAATTATCTAGATGA+AGG - chr5_2:71639911-71639930 Msa0786230:CDS 20.0%
!! AATATGATCATTACTTACAA+AGG + chr5_2:71640090-71640109 None:intergenic 20.0%
!! TCTATTTCACTTTATATTTC+AGG - chr5_2:71640732-71640751 Msa0786230:intron 20.0%
!! AATTATCTTTTTATTGGTGG+TGG - chr5_2:71639815-71639834 Msa0786230:intron 25.0%
! GAAATTCTTTCAAAACACAT+GGG - chr5_2:71639972-71639991 Msa0786230:CDS 25.0%
! AAAAGTAGGATGTTATTAAG+AGG + chr5_2:71640034-71640053 None:intergenic 25.0%
! ACATATATGAGAATTATGCA+AGG - chr5_2:71640471-71640490 Msa0786230:CDS 25.0%
! GAACGTTATATTGATAGATT+TGG - chr5_2:71640593-71640612 Msa0786230:intron 25.0%
!! AATTATCTTTTTATTGGTGG+TGG - chr5_2:71639815-71639834 Msa0786230:intron 25.0%
! GAAATTCTTTCAAAACACAT+GGG - chr5_2:71639972-71639991 Msa0786230:CDS 25.0%
! AAAAGTAGGATGTTATTAAG+AGG + chr5_2:71640034-71640053 None:intergenic 25.0%
! ACATATATGAGAATTATGCA+AGG - chr5_2:71640471-71640490 Msa0786230:CDS 25.0%
! GAACGTTATATTGATAGATT+TGG - chr5_2:71640593-71640612 Msa0786230:intron 25.0%
ATGGAATCAATGTTGTATTG+TGG + chr5_2:71639575-71639594 None:intergenic 30.0%
!!! AATGAAGTTTTGGAAAGAGT+TGG + chr5_2:71639609-71639628 None:intergenic 30.0%
! TGGAGAGATTAATGAAGTTT+TGG + chr5_2:71639619-71639638 None:intergenic 30.0%
GACAATGCATAAAATATGCA+AGG + chr5_2:71639860-71639879 None:intergenic 30.0%
!! TCGTACATGCAATATTTTGA+AGG + chr5_2:71639954-71639973 None:intergenic 30.0%
CGAAATTCTTTCAAAACACA+TGG - chr5_2:71639971-71639990 Msa0786230:CDS 30.0%
ATATCAATAGGTTTCCCATT+TGG - chr5_2:71640387-71640406 Msa0786230:intron 30.0%
! GTCAAGAAATTTGGTAACTA+TGG - chr5_2:71640500-71640519 Msa0786230:intron 30.0%
TGGAAATTACAATTATCCTG+AGG - chr5_2:71640520-71640539 Msa0786230:intron 30.0%
TCAATGTTGTATTGTGGATT+TGG + chr5_2:71639569-71639588 None:intergenic 30.0%
ATGGAATCAATGTTGTATTG+TGG + chr5_2:71639575-71639594 None:intergenic 30.0%
!!! AATGAAGTTTTGGAAAGAGT+TGG + chr5_2:71639609-71639628 None:intergenic 30.0%
! TGGAGAGATTAATGAAGTTT+TGG + chr5_2:71639619-71639638 None:intergenic 30.0%
GACAATGCATAAAATATGCA+AGG + chr5_2:71639860-71639879 None:intergenic 30.0%
!! TCGTACATGCAATATTTTGA+AGG + chr5_2:71639954-71639973 None:intergenic 30.0%
CGAAATTCTTTCAAAACACA+TGG - chr5_2:71639971-71639990 Msa0786230:CDS 30.0%
ATATCAATAGGTTTCCCATT+TGG - chr5_2:71640387-71640406 Msa0786230:intron 30.0%
! GTCAAGAAATTTGGTAACTA+TGG - chr5_2:71640500-71640519 Msa0786230:intron 30.0%
TGGAAATTACAATTATCCTG+AGG - chr5_2:71640520-71640539 Msa0786230:intron 30.0%
AGAGTTGGAGCAAAAGATTA+TGG + chr5_2:71639594-71639613 None:intergenic 35.0%
AATGTCTTAGTATTGCTCCA+AGG + chr5_2:71639665-71639684 None:intergenic 35.0%
TGGAGCAATACTAAGACATT+TGG - chr5_2:71639665-71639684 Msa0786230:three_prime_UTR 35.0%
AGACATTTGGACACAACAAT+AGG - chr5_2:71639678-71639697 Msa0786230:three_prime_UTR 35.0%
GACATTTGGACACAACAATA+GGG - chr5_2:71639679-71639698 Msa0786230:three_prime_UTR 35.0%
TGCATAAAATATGCAAGGTC+CGG + chr5_2:71639855-71639874 None:intergenic 35.0%
!!! ATCCTACTTTTTTGTGTACC+AGG - chr5_2:71640043-71640062 Msa0786230:CDS 35.0%
!! AGGATAAAGAAGCATTACTC+CGG + chr5_2:71640070-71640089 None:intergenic 35.0%
ATCATAGATACGCATCAATG+AGG - chr5_2:71640170-71640189 Msa0786230:intron 35.0%
TCATAGATACGCATCAATGA+GGG - chr5_2:71640171-71640190 Msa0786230:intron 35.0%
TATTGAAAGTCCTTCAACCT+TGG - chr5_2:71640195-71640214 Msa0786230:intron 35.0%
CAACTTACAAGGTCCTATAT+TGG - chr5_2:71640278-71640297 Msa0786230:CDS 35.0%
TTACAAGGTCCTATATTGGT+AGG - chr5_2:71640282-71640301 Msa0786230:CDS 35.0%
ATTGCTGAACCTACCAATAT+AGG + chr5_2:71640294-71640313 None:intergenic 35.0%
AGGACATACGTCAAGAAATT+TGG - chr5_2:71640491-71640510 Msa0786230:intron 35.0%
! TTTTATGTACATCGATCCCT+TGG - chr5_2:71640948-71640967 Msa0786230:five_prime_UTR 35.0%
AGAGTTGGAGCAAAAGATTA+TGG + chr5_2:71639594-71639613 None:intergenic 35.0%
AATGTCTTAGTATTGCTCCA+AGG + chr5_2:71639665-71639684 None:intergenic 35.0%
TGGAGCAATACTAAGACATT+TGG - chr5_2:71639665-71639684 Msa0786230:three_prime_UTR 35.0%
AGACATTTGGACACAACAAT+AGG - chr5_2:71639678-71639697 Msa0786230:three_prime_UTR 35.0%
GACATTTGGACACAACAATA+GGG - chr5_2:71639679-71639698 Msa0786230:three_prime_UTR 35.0%
TGCATAAAATATGCAAGGTC+CGG + chr5_2:71639855-71639874 None:intergenic 35.0%
!!! ATCCTACTTTTTTGTGTACC+AGG - chr5_2:71640043-71640062 Msa0786230:CDS 35.0%
!! AGGATAAAGAAGCATTACTC+CGG + chr5_2:71640070-71640089 None:intergenic 35.0%
ATCATAGATACGCATCAATG+AGG - chr5_2:71640170-71640189 Msa0786230:intron 35.0%
TCATAGATACGCATCAATGA+GGG - chr5_2:71640171-71640190 Msa0786230:intron 35.0%
TATTGAAAGTCCTTCAACCT+TGG - chr5_2:71640195-71640214 Msa0786230:intron 35.0%
CAACTTACAAGGTCCTATAT+TGG - chr5_2:71640278-71640297 Msa0786230:CDS 35.0%
TTACAAGGTCCTATATTGGT+AGG - chr5_2:71640282-71640301 Msa0786230:CDS 35.0%
ATTGCTGAACCTACCAATAT+AGG + chr5_2:71640294-71640313 None:intergenic 35.0%
AGGACATACGTCAAGAAATT+TGG - chr5_2:71640491-71640510 Msa0786230:intron 35.0%
! TTTTATGTACATCGATCCCT+TGG - chr5_2:71640948-71640967 Msa0786230:five_prime_UTR 35.0%
ACTTCATTAATCTCTCCACC+TGG - chr5_2:71639621-71639640 Msa0786230:three_prime_UTR 40.0%
TCCACCTGGAAAAATCATCA+TGG - chr5_2:71639635-71639654 Msa0786230:three_prime_UTR 40.0%
CCACCTGGAAAAATCATCAT+GGG - chr5_2:71639636-71639655 Msa0786230:three_prime_UTR 40.0%
! ACACCCATGATGATTTTTCC+AGG + chr5_2:71639642-71639661 None:intergenic 40.0%
AAAATCATCATGGGTGTCCT+TGG - chr5_2:71639645-71639664 Msa0786230:three_prime_UTR 40.0%
!!! ATCTTTTTATTGGTGGTGGC+CGG - chr5_2:71639819-71639838 Msa0786230:intron 40.0%
CATAAAATATGCAAGGTCCG+GGG + chr5_2:71639853-71639872 None:intergenic 40.0%
GCATAAAATATGCAAGGTCC+GGG + chr5_2:71639854-71639873 None:intergenic 40.0%
!! GGATAAAGAAGCATTACTCC+GGG + chr5_2:71640069-71640088 None:intergenic 40.0%
ACCAACAGTGGCTAACATAT+TGG - chr5_2:71640221-71640240 Msa0786230:intron 40.0%
CCAACAGTGGCTAACATATT+GGG - chr5_2:71640222-71640241 Msa0786230:intron 40.0%
CTTCGAGCTTTCAACTTACA+AGG - chr5_2:71640267-71640286 Msa0786230:CDS 40.0%
AGCTTCATGTATGGCCAAAT+GGG + chr5_2:71640404-71640423 None:intergenic 40.0%
AAGCTTCATGTATGGCCAAA+TGG + chr5_2:71640405-71640424 None:intergenic 40.0%
CAGAACACAAGCTTCATGTA+TGG + chr5_2:71640413-71640432 None:intergenic 40.0%
TTGGTACTGCCAATGTTCAA+TGG - chr5_2:71640440-71640459 Msa0786230:intron 40.0%
TAAGCTGCTCCATTGAACAT+TGG + chr5_2:71640452-71640471 None:intergenic 40.0%
! TTATCCTGAGGAGTACAAGA+AGG - chr5_2:71640532-71640551 Msa0786230:intron 40.0%
TCACTCTCTCAAATGCATCT+GGG + chr5_2:71640619-71640638 None:intergenic 40.0%
ATCACTCTCTCAAATGCATC+TGG + chr5_2:71640620-71640639 None:intergenic 40.0%
TCAATTTAACCAAGCTGGCA+TGG - chr5_2:71640788-71640807 Msa0786230:intron 40.0%
CAATTTAACCAAGCTGGCAT+GGG - chr5_2:71640789-71640808 Msa0786230:intron 40.0%
ACTTCATTAATCTCTCCACC+TGG - chr5_2:71639621-71639640 Msa0786230:three_prime_UTR 40.0%
TCCACCTGGAAAAATCATCA+TGG - chr5_2:71639635-71639654 Msa0786230:three_prime_UTR 40.0%
CCACCTGGAAAAATCATCAT+GGG - chr5_2:71639636-71639655 Msa0786230:three_prime_UTR 40.0%
! ACACCCATGATGATTTTTCC+AGG + chr5_2:71639642-71639661 None:intergenic 40.0%
AAAATCATCATGGGTGTCCT+TGG - chr5_2:71639645-71639664 Msa0786230:three_prime_UTR 40.0%
!!! ATCTTTTTATTGGTGGTGGC+CGG - chr5_2:71639819-71639838 Msa0786230:intron 40.0%
CATAAAATATGCAAGGTCCG+GGG + chr5_2:71639853-71639872 None:intergenic 40.0%
GCATAAAATATGCAAGGTCC+GGG + chr5_2:71639854-71639873 None:intergenic 40.0%
!! GGATAAAGAAGCATTACTCC+GGG + chr5_2:71640069-71640088 None:intergenic 40.0%
ACCAACAGTGGCTAACATAT+TGG - chr5_2:71640221-71640240 Msa0786230:intron 40.0%
CCAACAGTGGCTAACATATT+GGG - chr5_2:71640222-71640241 Msa0786230:intron 40.0%
CTTCGAGCTTTCAACTTACA+AGG - chr5_2:71640267-71640286 Msa0786230:CDS 40.0%
AGCTTCATGTATGGCCAAAT+GGG + chr5_2:71640404-71640423 None:intergenic 40.0%
AAGCTTCATGTATGGCCAAA+TGG + chr5_2:71640405-71640424 None:intergenic 40.0%
CAGAACACAAGCTTCATGTA+TGG + chr5_2:71640413-71640432 None:intergenic 40.0%
TTGGTACTGCCAATGTTCAA+TGG - chr5_2:71640440-71640459 Msa0786230:intron 40.0%
TAAGCTGCTCCATTGAACAT+TGG + chr5_2:71640452-71640471 None:intergenic 40.0%
! TTATCCTGAGGAGTACAAGA+AGG - chr5_2:71640532-71640551 Msa0786230:intron 40.0%
TCACTCTCTCAAATGCATCT+GGG + chr5_2:71640619-71640638 None:intergenic 40.0%
ATCACTCTCTCAAATGCATC+TGG + chr5_2:71640620-71640639 None:intergenic 40.0%
TCAATTTAACCAAGCTGGCA+TGG - chr5_2:71640788-71640807 Msa0786230:intron 40.0%
CAATTTAACCAAGCTGGCAT+GGG - chr5_2:71640789-71640808 Msa0786230:intron 40.0%
! CCCATGATGATTTTTCCAGG+TGG + chr5_2:71639639-71639658 None:intergenic 45.0%
!!! TCTTTTTATTGGTGGTGGCC+GGG - chr5_2:71639820-71639839 Msa0786230:intron 45.0%
! TGAGCTTAGCTCAGTTGGTA+TGG + chr5_2:71639882-71639901 None:intergenic 45.0%
GGCCTGGTACACAAAAAAGT+AGG + chr5_2:71640048-71640067 None:intergenic 45.0%
!!! ACTTTTTTGTGTACCAGGCC+CGG - chr5_2:71640048-71640067 Msa0786230:CDS 45.0%
TGTTGGTCATCCAAGGTTGA+AGG + chr5_2:71640208-71640227 None:intergenic 45.0%
CCCAATATGTTAGCCACTGT+TGG + chr5_2:71640225-71640244 None:intergenic 45.0%
TGAAGCTTGTGTTCTGCGTA+TGG - chr5_2:71640417-71640436 Msa0786230:intron 45.0%
AGAACCTTCTTGTACTCCTC+AGG + chr5_2:71640539-71640558 None:intergenic 45.0%
GAGCGTCAATTTAACCAAGC+TGG - chr5_2:71640783-71640802 Msa0786230:intron 45.0%
! CCCATGATGATTTTTCCAGG+TGG + chr5_2:71639639-71639658 None:intergenic 45.0%
!!! TCTTTTTATTGGTGGTGGCC+GGG - chr5_2:71639820-71639839 Msa0786230:intron 45.0%
! TGAGCTTAGCTCAGTTGGTA+TGG + chr5_2:71639882-71639901 None:intergenic 45.0%
GGCCTGGTACACAAAAAAGT+AGG + chr5_2:71640048-71640067 None:intergenic 45.0%
!!! ACTTTTTTGTGTACCAGGCC+CGG - chr5_2:71640048-71640067 Msa0786230:CDS 45.0%
TGTTGGTCATCCAAGGTTGA+AGG + chr5_2:71640208-71640227 None:intergenic 45.0%
CCCAATATGTTAGCCACTGT+TGG + chr5_2:71640225-71640244 None:intergenic 45.0%
TGAAGCTTGTGTTCTGCGTA+TGG - chr5_2:71640417-71640436 Msa0786230:intron 45.0%
AGAACCTTCTTGTACTCCTC+AGG + chr5_2:71640539-71640558 None:intergenic 45.0%
GAGCGTCAATTTAACCAAGC+TGG - chr5_2:71640783-71640802 Msa0786230:intron 45.0%
CAACCTTGGATGACCAACAG+TGG - chr5_2:71640209-71640228 Msa0786230:intron 50.0%
TAGCCACTGTTGGTCATCCA+AGG + chr5_2:71640215-71640234 None:intergenic 50.0%
GCTTGTGTTCTGCGTATGGT+TGG - chr5_2:71640421-71640440 Msa0786230:intron 50.0%
TTCTTCTTCCCATGCCAGCT+TGG + chr5_2:71640800-71640819 None:intergenic 50.0%
CAACCTTGGATGACCAACAG+TGG - chr5_2:71640209-71640228 Msa0786230:intron 50.0%
TAGCCACTGTTGGTCATCCA+AGG + chr5_2:71640215-71640234 None:intergenic 50.0%
GCTTGTGTTCTGCGTATGGT+TGG - chr5_2:71640421-71640440 Msa0786230:intron 50.0%
TTCTTCTTCCCATGCCAGCT+TGG + chr5_2:71640800-71640819 None:intergenic 50.0%
CCACGTGAGCTTAGCTCAGT+TGG + chr5_2:71639887-71639906 None:intergenic 55.0%
CCAACTGAGCTAAGCTCACG+TGG - chr5_2:71639884-71639903 Msa0786230:intron 55.0%
!! AAGAAGCATTACTCCGGGCC+TGG + chr5_2:71640064-71640083 None:intergenic 55.0%
CCACGTGAGCTTAGCTCAGT+TGG + chr5_2:71639887-71639906 None:intergenic 55.0%
CCAACTGAGCTAAGCTCACG+TGG - chr5_2:71639884-71639903 Msa0786230:intron 55.0%
!! AAGAAGCATTACTCCGGGCC+TGG + chr5_2:71640064-71640083 None:intergenic 55.0%
AAGGTCCGGGGTTCGAATCC+CGG + chr5_2:71639841-71639860 None:intergenic 60.0%
AAGGTCCGGGGTTCGAATCC+CGG + chr5_2:71639841-71639860 None:intergenic 60.0%
GGTGGCCGGGATTCGAACCC+CGG - chr5_2:71639833-71639852 Msa0786230:intron 70.0%
GGTGGCCGGGATTCGAACCC+CGG - chr5_2:71639833-71639852 Msa0786230:intron 70.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 71639567 71640990 71639567 ID=Msa0786230;Name=Msa0786230
chr5_2 mRNA 71639567 71640990 71639567 ID=Msa0786230-mRNA-1;Parent=Msa0786230;Name=Msa0786230-mRNA-1;_AED=0.10;_eAED=0.10;_QI=87|1|1|1|0.5|0.4|5|210|157
chr5_2 exon 71639567 71639803 71639567 ID=Msa0786230-mRNA-1:exon:21669;Parent=Msa0786230-mRNA-1
chr5_2 exon 71639911 71640160 71639911 ID=Msa0786230-mRNA-1:exon:21668;Parent=Msa0786230-mRNA-1
chr5_2 exon 71640258 71640380 71640258 ID=Msa0786230-mRNA-1:exon:21667;Parent=Msa0786230-mRNA-1
chr5_2 exon 71640466 71640492 71640466 ID=Msa0786230-mRNA-1:exon:21666;Parent=Msa0786230-mRNA-1
chr5_2 exon 71640857 71640990 71640857 ID=Msa0786230-mRNA-1:exon:21665;Parent=Msa0786230-mRNA-1
chr5_2 five_prime_UTR 71640904 71640990 71640904 ID=Msa0786230-mRNA-1:five_prime_utr;Parent=Msa0786230-mRNA-1
chr5_2 CDS 71640857 71640903 71640857 ID=Msa0786230-mRNA-1:cds;Parent=Msa0786230-mRNA-1
chr5_2 CDS 71640466 71640492 71640466 ID=Msa0786230-mRNA-1:cds;Parent=Msa0786230-mRNA-1
chr5_2 CDS 71640258 71640380 71640258 ID=Msa0786230-mRNA-1:cds;Parent=Msa0786230-mRNA-1
chr5_2 CDS 71639911 71640160 71639911 ID=Msa0786230-mRNA-1:cds;Parent=Msa0786230-mRNA-1
chr5_2 CDS 71639777 71639803 71639777 ID=Msa0786230-mRNA-1:cds;Parent=Msa0786230-mRNA-1
chr5_2 three_prime_UTR 71639567 71639776 71639567 ID=Msa0786230-mRNA-1:three_prime_utr;Parent=Msa0786230-mRNA-1
Gene Sequence

>Msa0786230

ATGGGTGTCCTTGGAGCAATACTAAGACATTTGGACACAACAATAGGGCCCGGAGTAATGCTTCTTTATCCTTTATACGCATCAATGAGGGCTATTGAAAGTCCTTCAACCTTGGATGACCAACAGTGGCTAACATATTGGGTTTTATATTCTTTCATAACTCTCTTCGAGCTTTCAACTTACAAGGTCCTATATTGGTTTCCCATTTGGCCATACATGAAGCTTGTGTTCTGCGTATGGTTGGTACTGCCAATGTTCAATGGAGCAGCTTACATATATGAGAATTATGCAAGGACATACGTCAAGAAATTTGGTAACTATGGAAATTACAATTATCCTGAGGAGTACAAGAAGGTTCTTCATATGATGACTTTCGATGCAAGAAAAGCAGTTGAACGTTATATTGATAGATTTGGCCCAGATGCATTTGAGAGAGTGATTAGAGCAGCTGAGAAAGAAGCAAAGAAGCATTGA
Protein sequence

>Msa0786230

MGVLGAILRHLDTTIGPGVMLLYPLYASMRAIESPSTLDDQQWLTYWVLYSFITLFELSTYKVLYWFPIWPYMKLVFCVWLVLPMFNGAAYIYENYARTYVKKFGNYGNYNYPEEYKKVLHMMTFDARKAVERYIDRFGPDAFERVIRAAEKEAKKH*