AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0627090

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0627090 MtrunA17_Chr4g0003281 99.430 351 2 0 1 351 1 351 0.0 726
Msa0627090 MtrunA17_Chr4g0064021 61.290 341 130 1 34 374 20 358 1.25e-151 433
Msa0627090 MtrunA17_Chr4g0043851 47.443 352 180 2 35 386 27 373 5.61e-114 338
Msa0627090 MtrunA17_Chr1g0189111 52.698 315 147 2 37 351 28 340 9.73e-114 338
Msa0627090 MtrunA17_Chr2g0321511 45.665 346 177 4 8 351 1 337 3.24e-111 330
Msa0627090 MtrunA17_Chr2g0285901 49.196 311 156 2 40 350 24 332 1.87e-104 313
Msa0627090 MtrunA17_Chr4g0026321 45.687 313 169 1 39 351 28 339 1.36e-103 311
Msa0627090 MtrunA17_Chr5g0439251 47.468 316 164 2 38 352 23 337 9.27e-98 299
Msa0627090 MtrunA17_Chr1g0177171 45.981 311 166 2 38 347 36 345 2.00e-94 291
Msa0627090 MtrunA17_Chr4g0034901 42.566 343 193 2 8 350 1 339 8.41e-93 286
Msa0627090 MtrunA17_Chr1g0155001 44.373 311 172 1 41 350 25 335 1.23e-91 281
Msa0627090 MtrunA17_Chr3g0144531 42.262 336 190 2 15 350 7 338 3.46e-90 279
Msa0627090 MtrunA17_Chr8g0339191 40.533 338 194 5 18 351 9 343 4.92e-89 276
Msa0627090 MtrunA17_Chr7g0234821 42.222 315 177 4 41 351 27 340 2.22e-88 275
Msa0627090 MtrunA17_Chr3g0119541 45.016 311 169 2 41 350 27 336 2.62e-88 271
Msa0627090 MtrunA17_Chr1g0146831 43.042 309 169 4 41 347 32 335 8.70e-87 268
Msa0627090 MtrunA17_Chr6g0487261 40.653 337 198 2 17 353 5 339 2.48e-86 271
Msa0627090 MtrunA17_Chr8g0389741 43.226 310 175 1 41 350 29 337 1.43e-84 266
Msa0627090 MtrunA17_Chr4g0046311 36.778 329 201 4 29 351 12 339 1.44e-76 244
Msa0627090 MtrunA17_Chr3g0110371 40.000 335 194 3 20 351 7 337 4.85e-76 243
Msa0627090 MtrunA17_Chr1g0161011 37.644 348 212 3 41 384 25 371 2.29e-74 239
Msa0627090 MtrunA17_Chr5g0434591 36.927 371 227 4 18 384 3 370 3.08e-74 239
Msa0627090 MtrunA17_Chr7g0223961 40.446 314 178 7 39 350 22 328 6.21e-74 237
Msa0627090 MtrunA17_Chr4g0075031 35.817 349 219 3 41 385 46 393 9.77e-72 233
Msa0627090 MtrunA17_Chr3g0121271 38.438 333 195 5 18 347 10 335 1.10e-70 230
Msa0627090 MtrunA17_Chr7g0250301 39.032 310 180 5 41 350 9 309 4.77e-69 220
Msa0627090 MtrunA17_Chr4g0038971 37.838 333 188 7 16 348 4 317 5.85e-68 218
Msa0627090 MtrunA17_Chr2g0294601 39.286 308 178 6 41 347 60 359 2.06e-67 218
Msa0627090 MtrunA17_Chr4g0038981 37.910 335 189 7 16 350 4 319 3.09e-67 216
Msa0627090 MtrunA17_Chr8g0378621 36.716 335 203 4 20 351 11 339 3.94e-67 221
Msa0627090 MtrunA17_Chr4g0039011 38.462 312 179 5 39 350 41 339 5.20e-67 216
Msa0627090 MtrunA17_Chr4g0043511 40.705 312 179 6 41 350 63 370 3.08e-66 219
Msa0627090 MtrunA17_Chr4g0039021 38.699 292 170 3 66 357 7 289 3.35e-66 212
Msa0627090 MtrunA17_Chr4g0038941 39.432 317 176 7 36 350 46 348 1.01e-65 213
Msa0627090 MtrunA17_Chr4g0039071 38.438 320 185 6 37 352 19 330 1.25e-64 211
Msa0627090 MtrunA17_Chr4g0038961 38.065 310 178 6 41 350 24 319 1.30e-64 209
Msa0627090 MtrunA17_Chr2g0294591 37.338 308 184 6 41 347 31 330 2.79e-61 201
Msa0627090 MtrunA17_Chr1g0174261 39.085 284 166 4 69 352 1 277 4.42e-60 198
Msa0627090 MtrunA17_Chr4g0069801 35.977 353 193 12 12 351 7 339 1.90e-57 195
Msa0627090 MtrunA17_Chr1g0148391 36.842 342 198 10 17 352 9 338 8.93e-56 190
Msa0627090 MtrunA17_Chr8g0383311 33.815 346 214 11 14 352 18 355 4.33e-54 186
Msa0627090 MtrunA17_Chr7g0250321 37.879 264 156 4 41 304 29 284 1.26e-53 179
Msa0627090 MtrunA17_Chr6g0461601 36.615 325 183 13 41 352 27 341 2.88e-51 178
Msa0627090 MtrunA17_Chr4g0062771 33.427 356 215 13 8 352 1 345 2.72e-50 176
Msa0627090 MtrunA17_Chr7g0253091 34.012 344 208 12 19 352 5 339 5.06e-50 175
Msa0627090 MtrunA17_Chr3g0138741 34.796 319 194 10 41 352 32 343 8.27e-49 172
Msa0627090 MtrunA17_Chr3g0105981 35.220 318 194 8 41 353 28 338 1.59e-48 170
Msa0627090 MtrunA17_Chr2g0290821 33.542 319 200 9 41 352 29 342 3.99e-48 170
Msa0627090 MtrunA17_Chr4g0076581 35.202 321 190 10 41 352 34 345 1.04e-46 166
Msa0627090 MtrunA17_Chr2g0306311 31.383 376 213 14 11 352 6 370 1.64e-45 163
Msa0627090 MtrunA17_Chr3g0129341 32.849 344 210 14 18 352 13 344 2.25e-43 157
Msa0627090 MtrunA17_Chr5g0436361 33.110 299 188 8 57 351 54 344 1.19e-39 145
Msa0627090 MtrunA17_Chr2g0294581 38.806 134 81 1 41 173 77 210 2.97e-21 92.8
Msa0627090 MtrunA17_Chr4g0018861 45.055 91 43 4 258 347 79 163 2.96e-15 74.3
Msa0627090 MtrunA17_Chr4g0018771 42.353 85 44 2 263 347 86 165 8.85e-15 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0627090 AT3G15800.1 73.270 318 84 1 35 351 38 355 6.95e-173 488
Msa0627090 AT2G26600.1 62.776 317 118 0 35 351 29 345 2.13e-151 433
Msa0627090 AT2G26600.2 63.415 246 90 0 106 351 6 251 2.97e-118 345
Msa0627090 AT5G42100.2 48.788 330 161 4 25 351 16 340 5.12e-107 322
Msa0627090 AT5G42100.1 48.788 330 161 4 25 351 16 340 9.67e-107 321
Msa0627090 AT2G27500.2 46.795 312 166 0 41 352 30 341 1.47e-106 318
Msa0627090 AT2G27500.1 46.795 312 166 0 41 352 30 341 4.74e-106 318
Msa0627090 AT1G30080.2 49.677 310 155 1 41 350 37 345 6.04e-106 317
Msa0627090 AT1G30080.1 49.677 310 155 1 41 350 37 345 1.03e-105 318
Msa0627090 AT4G18340.1 43.360 369 202 4 20 387 16 378 7.82e-105 315
Msa0627090 AT4G18340.2 43.631 369 201 4 20 387 16 378 8.93e-105 314
Msa0627090 AT1G32860.1 47.619 315 162 2 39 351 28 341 3.65e-102 309
Msa0627090 AT4G26830.1 46.429 336 173 3 13 347 2 331 5.27e-98 299
Msa0627090 AT4G26830.2 47.756 312 160 2 37 347 26 335 3.08e-96 295
Msa0627090 AT2G05790.1 46.815 314 165 2 38 350 23 335 9.65e-94 289
Msa0627090 AT4G34480.2 47.097 310 164 0 41 350 27 336 4.20e-93 283
Msa0627090 AT5G42720.1 46.945 311 164 1 41 350 27 337 2.06e-92 285
Msa0627090 AT2G16230.1 44.839 310 171 0 41 350 26 335 3.95e-92 286
Msa0627090 AT5G55180.2 44.444 315 171 3 35 347 22 334 3.19e-91 282
Msa0627090 AT5G55180.1 44.444 315 171 3 35 347 22 334 3.78e-91 282
Msa0627090 AT4G34480.1 47.097 310 164 0 41 350 27 336 6.71e-91 283
Msa0627090 AT5G24318.4 41.742 333 178 6 20 350 19 337 6.80e-91 278
Msa0627090 AT2G27500.3 47.710 262 137 0 91 352 2 263 3.57e-89 272
Msa0627090 AT5G24318.2 40.179 336 194 4 17 350 7 337 4.92e-89 276
Msa0627090 AT5G24318.1 40.179 336 194 4 17 350 7 337 6.42e-89 276
Msa0627090 AT3G07320.1 41.250 320 183 4 36 351 21 339 1.47e-86 270
Msa0627090 AT5G56590.1 41.009 317 186 1 35 350 21 337 1.12e-83 264
Msa0627090 AT2G01630.2 38.506 348 209 3 41 384 24 370 2.28e-80 252
Msa0627090 AT2G01630.3 38.506 348 209 3 41 384 24 370 8.39e-80 252
Msa0627090 AT2G01630.1 38.506 348 209 3 41 384 24 370 9.00e-79 251
Msa0627090 AT4G29360.2 42.173 313 180 1 41 353 38 349 1.06e-76 247
Msa0627090 AT4G29360.1 41.231 325 190 1 29 353 16 339 1.11e-76 247
Msa0627090 AT3G23770.1 36.842 323 197 4 35 351 32 353 3.70e-75 241
Msa0627090 AT3G13560.4 40.491 326 187 4 29 351 18 339 3.70e-73 237
Msa0627090 AT3G13560.1 40.491 326 187 4 29 351 18 339 3.70e-73 237
Msa0627090 AT3G13560.2 40.491 326 187 4 29 351 18 339 3.70e-73 237
Msa0627090 AT3G13560.5 40.491 326 187 4 29 351 18 339 3.70e-73 237
Msa0627090 AT3G13560.3 40.491 326 187 4 29 351 18 339 3.70e-73 237
Msa0627090 AT4G14080.1 36.392 316 193 5 41 351 43 355 5.99e-72 233
Msa0627090 AT1G11820.3 36.103 349 218 3 41 385 45 392 1.25e-71 232
Msa0627090 AT1G66250.1 39.185 319 188 4 38 352 29 345 3.33e-71 232
Msa0627090 AT1G11820.2 36.103 349 218 3 41 385 45 392 3.76e-71 232
Msa0627090 AT1G11820.1 38.387 310 187 2 41 347 45 353 5.41e-71 228
Msa0627090 AT3G46570.1 40.171 351 185 13 9 350 1 335 4.62e-68 219
Msa0627090 AT5G20390.1 38.835 309 179 6 41 348 34 333 5.12e-67 216
Msa0627090 AT3G61810.1 34.385 317 193 4 41 351 56 363 6.04e-64 209
Msa0627090 AT4G16260.1 35.455 330 200 6 20 347 4 322 9.94e-64 207
Msa0627090 AT4G34480.3 49.776 223 112 0 128 350 3 225 5.25e-63 207
Msa0627090 AT3G57270.1 36.656 311 188 5 38 347 25 327 2.29e-62 204
Msa0627090 AT1G77780.1 36.667 330 195 8 20 348 7 323 3.09e-62 204
Msa0627090 AT3G57260.1 35.435 333 200 7 18 350 12 329 8.35e-61 200
Msa0627090 AT3G57260.2 35.435 333 200 7 18 350 28 345 1.15e-60 200
Msa0627090 AT4G16260.2 35.644 303 182 6 47 347 2 293 4.99e-60 197
Msa0627090 AT5G20340.1 36.246 309 187 6 41 348 44 343 2.46e-59 196
Msa0627090 AT2G39640.1 35.905 337 203 8 15 350 7 331 5.56e-59 201
Msa0627090 AT3G55430.1 38.170 317 177 12 41 350 30 334 7.88e-58 195
Msa0627090 AT5G20330.1 36.570 309 186 6 41 348 35 334 1.13e-57 192
Msa0627090 AT1G33220.1 34.337 332 208 6 8 337 1 324 6.67e-57 189
Msa0627090 AT1G77790.1 37.380 313 184 7 41 350 31 334 1.26e-56 189
Msa0627090 AT5G24318.3 44.279 201 107 2 151 350 2 198 1.27e-54 183
Msa0627090 AT3G57240.1 34.194 310 190 6 38 347 33 328 2.36e-54 183
Msa0627090 AT5G64790.1 35.000 340 205 10 20 351 8 339 2.49e-54 187
Msa0627090 AT5G20870.1 37.152 323 186 10 41 351 31 348 2.66e-51 179
Msa0627090 AT4G31140.1 35.535 318 193 9 41 352 28 339 1.74e-50 177
Msa0627090 AT1G64760.1 35.174 344 202 12 20 352 4 337 3.13e-50 176
Msa0627090 AT1G64760.2 35.174 344 202 12 20 352 4 337 3.13e-50 176
Msa0627090 AT5G20560.1 33.036 336 212 7 18 348 8 335 2.46e-49 170
Msa0627090 AT2G19440.2 36.137 321 187 12 41 352 22 333 1.18e-48 172
Msa0627090 AT2G19440.1 36.137 321 187 12 41 352 22 333 1.18e-48 172
Msa0627090 AT5G18220.1 32.759 348 213 11 11 352 8 340 2.36e-48 171
Msa0627090 AT5G58090.1 34.385 317 196 9 41 351 23 333 1.63e-47 169
Msa0627090 AT3G04010.1 35.514 321 189 11 41 352 34 345 3.34e-47 168
Msa0627090 AT3G24330.1 33.983 359 206 16 5 352 15 353 7.96e-47 167
Msa0627090 AT5G58480.1 33.333 345 205 12 30 358 12 347 4.07e-44 159
Msa0627090 AT4G17180.1 32.537 335 203 12 27 352 12 332 2.82e-43 157
Msa0627090 AT3G55780.1 32.237 304 191 9 54 350 49 344 2.51e-40 147

Find 67 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACCATCTTCATGTACATTT+AGG 0.162171 4_3:-7175968 Msa0627090:CDS
CCTCGTGTTCATTCTTTCTT+TGG 0.185604 4_3:-7176943 Msa0627090:CDS
AATTATAAATTGCAGGGAAT+TGG 0.233656 4_3:-7174413 Msa0627090:intron
GTGGTGGAAATATTGAAGTT+TGG 0.257640 4_3:-7176106 Msa0627090:CDS
AGAAGTGTAACCACACTTTC+AGG 0.271942 4_3:+7176353 None:intergenic
GTCATAATGTAGTTTACTTT+TGG 0.276964 4_3:+7175785 None:intergenic
GGGTATGCATTTATATAAAA+AGG 0.279248 4_3:+7175888 None:intergenic
ATTGTCTTTAATCCAGTTCA+TGG 0.298902 4_3:+7176190 None:intergenic
TTTATTTAGAAAATTGTTAC+AGG 0.303318 4_3:+7174277 None:intergenic
TTCTAAATAAATTGAATGTA+TGG 0.317709 4_3:-7174265 Msa0627090:three_prime_UTR
ACCATCTTCATGTACATTTA+GGG 0.319187 4_3:-7175967 Msa0627090:CDS
ATAGGCCTAAAATGTTGGTT+AGG 0.346325 4_3:-7175566 Msa0627090:CDS
GAAATTTGGGTTTGATAAGA+TGG 0.348519 4_3:-7175718 Msa0627090:CDS
TGGTGGAAATATTGAAGTTT+GGG 0.349355 4_3:-7176105 Msa0627090:CDS
ACCTTACATGAAACCTTTGT+TGG 0.369026 4_3:-7175934 Msa0627090:CDS
ATTATAAATTGCAGGGAATT+GGG 0.372588 4_3:-7174412 Msa0627090:intron
TTAGGGAACAAAGCATTTAC+AGG 0.386581 4_3:-7176422 Msa0627090:CDS
TGTCTTTCTGATCCTGGTCC+AGG 0.404190 4_3:+7175507 None:intergenic
GATTGTTTCTGAGACTGGTT+GGG 0.414418 4_3:-7175691 Msa0627090:CDS
TCCAACAAAGGTTTCATGTA+AGG 0.416560 4_3:+7175933 None:intergenic
CTAAGTGCCTTCAAAGGATC+TGG 0.417558 4_3:-7176278 Msa0627090:CDS
GCGAAGATCGAGCCATGAAC+TGG 0.421344 4_3:-7176202 Msa0627090:CDS
AAAGCATTTACAGGAACATA+TGG 0.426078 4_3:-7176413 Msa0627090:CDS
CAGATCCTTTGAAGGCACTT+AGG 0.437728 4_3:+7176279 None:intergenic
TCCTATTCCAGATCCTTTGA+AGG 0.453072 4_3:+7176271 None:intergenic
AGAGAAAAGGAACACCTCAT+AGG 0.455796 4_3:-7175584 Msa0627090:CDS
TTCAATGAGAATTTGAAGCC+TGG 0.457788 4_3:-7175525 Msa0627090:CDS
AAAATAGTTGGAATTGCCAT+TGG 0.458980 4_3:-7176143 Msa0627090:CDS
TAGGCCTAAAATGTTGGTTA+GGG 0.461164 4_3:-7175565 Msa0627090:CDS
CCAAAGTGTCTTTCTGATCC+TGG 0.464550 4_3:+7175501 None:intergenic
TTTGGATTATTTAATCATGA+TGG 0.466918 4_3:-7175483 Msa0627090:CDS
AAGAGGTTACTTAAGAGAAA+AGG 0.468730 4_3:-7175597 Msa0627090:CDS
GCTAAGACTTCAACATCAAA+TGG 0.473387 4_3:-7176975 Msa0627090:CDS
AATGTTCAACCATATCTTCC+AGG 0.475716 4_3:-7176170 Msa0627090:CDS
GCCTTCAAAGGATCTGGAAT+AGG 0.476160 4_3:-7176272 Msa0627090:CDS
GGAACATATGGAGTAAACTA+TGG 0.476388 4_3:-7176401 Msa0627090:CDS
GAAGTGTAACCACACTTTCA+GGG 0.479495 4_3:+7176354 None:intergenic
ACCTAAAATTTCATTTCCAA+TGG 0.483236 4_3:+7176127 None:intergenic
TTGCTAGGCCAAGTCTATCA+AGG 0.491645 4_3:+7176045 None:intergenic
GAAGTGATTGTTTCTGAGAC+TGG 0.499216 4_3:-7175696 Msa0627090:CDS
AATTGGGTCTTCATACATGA+TGG 0.501687 4_3:-7174396 Msa0627090:CDS
AAAGTACCTACCTAGAGAAA+AGG 0.503724 4_3:+7176901 None:intergenic
TGATTGTTTCTGAGACTGGT+TGG 0.504115 4_3:-7175692 Msa0627090:CDS
GAGACTGGTTGGGCCTCTCA+TGG 0.506281 4_3:-7175681 Msa0627090:CDS
ATGTCTGTCAAAAGTTCGTT+CGG 0.507994 4_3:+7176233 None:intergenic
AAACCTCAATTTGCTTTGCT+AGG 0.509251 4_3:+7176030 None:intergenic
GAGAATTTGAAGCCTGGACC+AGG 0.516130 4_3:-7175519 Msa0627090:CDS
GCAAATACAGCTTCTGAATG+TGG 0.522800 4_3:+7176002 None:intergenic
TGGCCTAGCAAAGCAAATTG+AGG 0.526445 4_3:-7176033 Msa0627090:CDS
CATGGTGATGATAATGAAGC+TGG 0.527812 4_3:-7175663 Msa0627090:CDS
TTGAAGGCACTTAGGACTTG+AGG 0.530321 4_3:+7176287 None:intergenic
AGTTTATGTCAATATGTTGA+GGG 0.534313 4_3:+7175844 None:intergenic
CCAGGATCAGAAAGACACTT+TGG 0.537832 4_3:-7175501 Msa0627090:CDS
TCCCTAAATGTACATGAAGA+TGG 0.548834 4_3:+7175966 None:intergenic
TAGTTTATGTCAATATGTTG+AGG 0.553478 4_3:+7175843 None:intergenic
ACCTCATAGGCCTAAAATGT+TGG 0.556828 4_3:-7175571 Msa0627090:CDS
TATAGTGCCCTTGATAGACT+TGG 0.566519 4_3:-7176053 Msa0627090:CDS
TTCATTATCATCACCATGAG+AGG 0.570975 4_3:+7175668 None:intergenic
TGTAAGAAAACAAATTAGAG+AGG 0.586256 4_3:+7174184 None:intergenic
CAAGTCCTAAGTGCCTTCAA+AGG 0.599078 4_3:-7176284 Msa0627090:CDS
AAGTGTAACCACACTTTCAG+GGG 0.600505 4_3:+7176355 None:intergenic
TTAGTGTTAACAAATTATGG+AGG 0.603875 4_3:+7174158 None:intergenic
TGCTAGGCCAAGTCTATCAA+GGG 0.613695 4_3:+7176046 None:intergenic
CTAAATGTACATGAAGATGG+TGG 0.619425 4_3:+7175969 None:intergenic
CCAAAGAAAGAATGAACACG+AGG 0.636038 4_3:+7176943 None:intergenic
TTATTTAGAAAATTGTTACA+GGG 0.644962 4_3:+7174278 None:intergenic
AGTGTAACCACACTTTCAGG+GGG 0.724067 4_3:+7176356 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTTTAATATAAAAATTGTA+AGG + chr4_3:7174616-7174635 None:intergenic 10.0%
!!! TTACAATTTTTATATTAAAG+TGG - chr4_3:7174615-7174634 Msa0627090:intron 10.0%
!!! TAAGTAAATTTTTTTTTGTA+GGG + chr4_3:7176119-7176138 None:intergenic 10.0%
!!! ATAAGTAAATTTTTTTTTGT+AGG + chr4_3:7176120-7176139 None:intergenic 10.0%
!!! AAACAAAATTTATTTTAGTA+GGG + chr4_3:7176152-7176171 None:intergenic 10.0%
!!! TAAACAAAATTTATTTTAGT+AGG + chr4_3:7176153-7176172 None:intergenic 10.0%
!! TTAAAAAATTTAAGAGTTTA+AGG + chr4_3:7176181-7176200 None:intergenic 10.0%
!! TGTTATATACATTAAAAAAA+GGG + chr4_3:7176577-7176596 None:intergenic 10.0%
!! ATGTTATATACATTAAAAAA+AGG + chr4_3:7176578-7176597 None:intergenic 10.0%
!! AATCAAATTTAAAAAATTTG+AGG - chr4_3:7176608-7176627 Msa0627090:intron 10.0%
!! ATCAAATTTAAAAAATTTGA+GGG - chr4_3:7176609-7176628 Msa0627090:intron 10.0%
!! AATAGAATAAACTTGAAATT+TGG - chr4_3:7174495-7174514 Msa0627090:intron 15.0%
!!! CTTTCTCAAATTTTTATTAT+AGG + chr4_3:7175542-7175561 None:intergenic 15.0%
!! ATAATAAAAATTTGAGAAAG+AGG - chr4_3:7175542-7175561 Msa0627090:CDS 15.0%
!! AATGCTATTAAAATTAAGTA+AGG + chr4_3:7176090-7176109 None:intergenic 15.0%
!! AGATATAAGTAACAAAAATA+TGG - chr4_3:7176312-7176331 Msa0627090:CDS 15.0%
!!! AATTTTTGCGATATTTTTTT+CGG - chr4_3:7176347-7176366 Msa0627090:CDS 15.0%
!! AAGCATAAATTAAACAAATT+TGG - chr4_3:7176431-7176450 Msa0627090:intron 15.0%
!! AATGCTATTAAAATAAGATA+AGG - chr4_3:7176503-7176522 Msa0627090:intron 15.0%
!!! TTTTTTAAATTTGATTCTGA+TGG + chr4_3:7176604-7176623 None:intergenic 15.0%
!! TTATTTAGAAAATTGTTACA+GGG + chr4_3:7176881-7176900 None:intergenic 15.0%
!! TTTATTTAGAAAATTGTTAC+AGG + chr4_3:7176882-7176901 None:intergenic 15.0%
!! TTCTAAATAAATTGAATGTA+TGG - chr4_3:7176891-7176910 Msa0627090:intron 15.0%
!! TTTATGTAATTATACCACTA+TGG - chr4_3:7174344-7174363 Msa0627090:CDS 20.0%
!! TTATATTAAAGTGGTGATTT+AGG - chr4_3:7174624-7174643 Msa0627090:intron 20.0%
!!! TTGGAATTTTTTTCACAAAT+TGG - chr4_3:7175241-7175260 Msa0627090:intron 20.0%
!! TTTGGATTATTTAATCATGA+TGG - chr4_3:7175673-7175692 Msa0627090:CDS 20.0%
!! AAAATGTAGAAAGGAAAAAA+TGG + chr4_3:7175781-7175800 None:intergenic 20.0%
!! TACAAAAACAAAATGTAGAA+AGG + chr4_3:7175790-7175809 None:intergenic 20.0%
!!! CTTATGACGTTTTTTTTTAA+GGG - chr4_3:7176016-7176035 Msa0627090:CDS 20.0%
!!! TTAAGGGTTAATTATGTTTT+TGG - chr4_3:7176032-7176051 Msa0627090:CDS 20.0%
!!! GTTTTTCAACAATTTTTTAC+AGG + chr4_3:7176401-7176420 None:intergenic 20.0%
!!! AAAAGGGTTAATTATGTTTT+TGG + chr4_3:7176561-7176580 None:intergenic 20.0%
!! TTCTAACAATTATAAATTGC+AGG - chr4_3:7176736-7176755 Msa0627090:intron 20.0%
!! TCTAACAATTATAAATTGCA+GGG - chr4_3:7176737-7176756 Msa0627090:intron 20.0%
! CTTTGATGTTTCATAGAAAA+AGG - chr4_3:7174299-7174318 Msa0627090:exon 25.0%
!! AAGGTACTTATTTGCAATTA+AGG - chr4_3:7174318-7174337 Msa0627090:CDS 25.0%
! CCAAAGAAAAATTTGAAAGT+AGG + chr4_3:7174707-7174726 None:intergenic 25.0%
!! CCTACTTTCAAATTTTTCTT+TGG - chr4_3:7174704-7174723 Msa0627090:intron 25.0%
! ACCTAAAATTTCATTTCCAA+TGG + chr4_3:7175032-7175051 None:intergenic 25.0%
!! ATTGGAAATGAAATTTTAGG+TGG - chr4_3:7175031-7175050 Msa0627090:intron 25.0%
! TGTGAAAAAAATTCCAACAA+AGG + chr4_3:7175238-7175257 None:intergenic 25.0%
! GGGTATGCATTTATATAAAA+AGG + chr4_3:7175271-7175290 None:intergenic 25.0%
!!! TATAAATGCATACCCTTTTT+TGG - chr4_3:7175276-7175295 Msa0627090:intron 25.0%
!! CATTTTTATAGGCCAAAAAA+GGG + chr4_3:7175291-7175310 None:intergenic 25.0%
!!! TCATTTTTATAGGCCAAAAA+AGG + chr4_3:7175292-7175311 None:intergenic 25.0%
! AGTTTATGTCAATATGTTGA+GGG + chr4_3:7175315-7175334 None:intergenic 25.0%
! TAGTTTATGTCAATATGTTG+AGG + chr4_3:7175316-7175335 None:intergenic 25.0%
!! GTCATAATGTAGTTTACTTT+TGG + chr4_3:7175374-7175393 None:intergenic 25.0%
!! TATTTTGCATTGGAGAAATT+TGG - chr4_3:7175424-7175443 Msa0627090:intron 25.0%
!! ATTTTGCATTGGAGAAATTT+GGG - chr4_3:7175425-7175444 Msa0627090:intron 25.0%
! CTCTCTACGATAAATAATTA+AGG + chr4_3:7175887-7175906 None:intergenic 25.0%
! CCTTAAAAAAAAACGTCATA+AGG + chr4_3:7176018-7176037 None:intergenic 25.0%
!!! CCTTATGACGTTTTTTTTTA+AGG - chr4_3:7176015-7176034 Msa0627090:CDS 25.0%
! GTGTTAAGAACGTTATTAAA+AGG + chr4_3:7176377-7176396 None:intergenic 25.0%
! AAACAAATTTGGATTGAGTA+AGG - chr4_3:7176442-7176461 Msa0627090:intron 25.0%
! AGATAAGGACTAAATTTACA+AGG - chr4_3:7176518-7176537 Msa0627090:intron 25.0%
! AATTATAAATTGCAGGGAAT+TGG - chr4_3:7176743-7176762 Msa0627090:intron 25.0%
! ATTATAAATTGCAGGGAATT+GGG - chr4_3:7176744-7176763 Msa0627090:intron 25.0%
! TGTAAGAAAACAAATTAGAG+AGG + chr4_3:7176975-7176994 None:intergenic 25.0%
! TTAGTGTTAACAAATTATGG+AGG + chr4_3:7177001-7177020 None:intergenic 25.0%
GTGAGATGTTTCATAGAAAA+AGG - chr4_3:7174366-7174385 Msa0627090:CDS 30.0%
GTCAATACATATGTAGTTGT+TGG - chr4_3:7174419-7174438 Msa0627090:intron 30.0%
TTGTTTGCTTTCTATACCAT+CGG + chr4_3:7174453-7174472 None:intergenic 30.0%
!! TTTTTCTTTGGTGCAGTATT+AGG - chr4_3:7174716-7174735 Msa0627090:intron 30.0%
!! TTTTCTTTGGTGCAGTATTA+GGG - chr4_3:7174717-7174736 Msa0627090:intron 30.0%
! AAAGCATTTACAGGAACATA+TGG - chr4_3:7174743-7174762 Msa0627090:intron 30.0%
ATTGTCTTTAATCCAGTTCA+TGG + chr4_3:7174969-7174988 None:intergenic 30.0%
! ATTTTTGTTCCTGGAAGATA+TGG + chr4_3:7174998-7175017 None:intergenic 30.0%
!!! ATTCCAACTATTTTTGTTCC+TGG + chr4_3:7175007-7175026 None:intergenic 30.0%
AAAATAGTTGGAATTGCCAT+TGG - chr4_3:7175013-7175032 Msa0627090:intron 30.0%
! GCCATTGGAAATGAAATTTT+AGG - chr4_3:7175028-7175047 Msa0627090:intron 30.0%
TGGTGGAAATATTGAAGTTT+GGG - chr4_3:7175051-7175070 Msa0627090:intron 30.0%
!!! CTATAGATATTTTTTGCAGC+AGG + chr4_3:7175088-7175107 None:intergenic 30.0%
ACCATCTTCATGTACATTTA+GGG - chr4_3:7175189-7175208 Msa0627090:intron 30.0%
!!! ATGTTGAGGGTCATTTTTAT+AGG + chr4_3:7175302-7175321 None:intergenic 30.0%
!! GCTTTATTTAAGAAAAACCC+TGG - chr4_3:7175337-7175356 Msa0627090:intron 30.0%
! GAAATTTGGGTTTGATAAGA+TGG - chr4_3:7175438-7175457 Msa0627090:intron 30.0%
AAGAGGTTACTTAAGAGAAA+AGG - chr4_3:7175559-7175578 Msa0627090:CDS 30.0%
! TAAACCCTAACCAACATTTT+AGG + chr4_3:7175598-7175617 None:intergenic 30.0%
!! GGAAGTATTGCATATGATAT+TGG - chr4_3:7175694-7175713 Msa0627090:CDS 30.0%
GCATATGATATTGGATTTAC+TGG - chr4_3:7175703-7175722 Msa0627090:CDS 30.0%
!! AAATGGATTACATACCTTGA+AGG + chr4_3:7175764-7175783 None:intergenic 30.0%
AAGTAAGGACTAAACTTACA+AGG + chr4_3:7176075-7176094 None:intergenic 30.0%
! CAAGTTTAGAACACTATGAA+AGG - chr4_3:7176215-7176234 Msa0627090:CDS 30.0%
!!! TTTTGCGATATTTTTTTCGG+AGG - chr4_3:7176350-7176369 Msa0627090:CDS 30.0%
!!! TGAGTGCAGAAATTTTTTGT+AGG - chr4_3:7176473-7176492 Msa0627090:intron 30.0%
!!! GAGTGCAGAAATTTTTTGTA+GGG - chr4_3:7176474-7176493 Msa0627090:intron 30.0%
GTGTGAGCTATAAATATAGT+TGG + chr4_3:7176653-7176672 None:intergenic 30.0%
GATAAAACCAACCTAAATCA+TGG + chr4_3:7176688-7176707 None:intergenic 30.0%
AAGCATGTTAGTTATGCATA+AGG + chr4_3:7176710-7176729 None:intergenic 30.0%
GCTAAGACTTCAACATCAAA+TGG - chr4_3:7174181-7174200 Msa0627090:three_prime_UTR 35.0%
! TTATCAGCAACCTTTTCTCT+AGG - chr4_3:7174245-7174264 Msa0627090:three_prime_UTR 35.0%
! AAAGTACCTACCTAGAGAAA+AGG + chr4_3:7174258-7174277 None:intergenic 35.0%
TATGAAACATCTCACCATAG+TGG + chr4_3:7174361-7174380 None:intergenic 35.0%
ATCGTTAGATCAACATCAGA+CGG + chr4_3:7174522-7174541 None:intergenic 35.0%
!! ATGCATTGCTTTTCATGACA+TGG - chr4_3:7174672-7174691 Msa0627090:intron 35.0%
! TTAGGGAACAAAGCATTTAC+AGG - chr4_3:7174734-7174753 Msa0627090:intron 35.0%
GGAACATATGGAGTAAACTA+TGG - chr4_3:7174755-7174774 Msa0627090:intron 35.0%
ATGTCTGTCAAAAGTTCGTT+CGG + chr4_3:7174926-7174945 None:intergenic 35.0%
! AACGAACTTTTGACAGACAT+AGG - chr4_3:7174926-7174945 Msa0627090:intron 35.0%
AATGTTCAACCATATCTTCC+AGG - chr4_3:7174986-7175005 Msa0627090:intron 35.0%
CTTCCAGGAACAAAAATAGT+TGG - chr4_3:7175001-7175020 Msa0627090:intron 35.0%
!! GGAAATGAAATTTTAGGTGG+TGG - chr4_3:7175034-7175053 Msa0627090:intron 35.0%
! GTGGTGGAAATATTGAAGTT+TGG - chr4_3:7175050-7175069 Msa0627090:intron 35.0%
! AAACCTCAATTTGCTTTGCT+AGG + chr4_3:7175129-7175148 None:intergenic 35.0%
CTAAATGTACATGAAGATGG+TGG + chr4_3:7175190-7175209 None:intergenic 35.0%
CACCATCTTCATGTACATTT+AGG - chr4_3:7175188-7175207 Msa0627090:intron 35.0%
TCCCTAAATGTACATGAAGA+TGG + chr4_3:7175193-7175212 None:intergenic 35.0%
ACCTTACATGAAACCTTTGT+TGG - chr4_3:7175222-7175241 Msa0627090:intron 35.0%
TCCAACAAAGGTTTCATGTA+AGG + chr4_3:7175226-7175245 None:intergenic 35.0%
! TTTTGGCATCATAAATGCCA+GGG + chr4_3:7175357-7175376 None:intergenic 35.0%
!!! TGATGCTGCTTATTTTGCAT+TGG - chr4_3:7175414-7175433 Msa0627090:intron 35.0%
TTCATTATCATCACCATGAG+AGG + chr4_3:7175491-7175510 None:intergenic 35.0%
! ATAGGCCTAAAATGTTGGTT+AGG - chr4_3:7175590-7175609 Msa0627090:CDS 35.0%
! TAGGCCTAAAATGTTGGTTA+GGG - chr4_3:7175591-7175610 Msa0627090:CDS 35.0%
! TTCAATGAGAATTTGAAGCC+TGG - chr4_3:7175631-7175650 Msa0627090:CDS 35.0%
GATTACATACCTTGAAGGAA+AGG + chr4_3:7175759-7175778 None:intergenic 35.0%
! TACAAGGTCGCAATTTTGTA+GGG + chr4_3:7176534-7176553 None:intergenic 35.0%
! TTACAAGGTCGCAATTTTGT+AGG + chr4_3:7176533-7176552 None:intergenic 35.0%
! TACAAGGTCGCAATTTTGTA+TGG - chr4_3:7176534-7176553 Msa0627090:intron 35.0%
AGCATACTAGTCCATGATTT+AGG - chr4_3:7176674-7176693 Msa0627090:intron 35.0%
TACTAGTCCATGATTTAGGT+TGG - chr4_3:7176678-7176697 Msa0627090:intron 35.0%
AATTGGGTCTTCATACATGA+TGG - chr4_3:7176760-7176779 Msa0627090:intron 35.0%
CCAAAGAAAGAATGAACACG+AGG + chr4_3:7174216-7174235 None:intergenic 40.0%
CCTCGTGTTCATTCTTTCTT+TGG - chr4_3:7174213-7174232 Msa0627090:three_prime_UTR 40.0%
!! GTATTGACTACGTGAATGTG+TGG + chr4_3:7174407-7174426 None:intergenic 40.0%
TATTCATGCAGTCAGCACAT+TGG + chr4_3:7174547-7174566 None:intergenic 40.0%
AAGTGTAACCACACTTTCAG+GGG + chr4_3:7174804-7174823 None:intergenic 40.0%
GAAGTGTAACCACACTTTCA+GGG + chr4_3:7174805-7174824 None:intergenic 40.0%
! AGAAGTGTAACCACACTTTC+AGG + chr4_3:7174806-7174825 None:intergenic 40.0%
TCCTATTCCAGATCCTTTGA+AGG + chr4_3:7174888-7174907 None:intergenic 40.0%
! CTTTTGACAGACATAGGTGT+AGG - chr4_3:7174932-7174951 Msa0627090:intron 40.0%
TATAGTGCCCTTGATAGACT+TGG - chr4_3:7175103-7175122 Msa0627090:intron 40.0%
GCAAATACAGCTTCTGAATG+TGG + chr4_3:7175157-7175176 None:intergenic 40.0%
! CTTTTGGCATCATAAATGCC+AGG + chr4_3:7175358-7175377 None:intergenic 40.0%
!! GAAGTGATTGTTTCTGAGAC+TGG - chr4_3:7175460-7175479 Msa0627090:intron 40.0%
!! TGATTGTTTCTGAGACTGGT+TGG - chr4_3:7175464-7175483 Msa0627090:intron 40.0%
!! GATTGTTTCTGAGACTGGTT+GGG - chr4_3:7175465-7175484 Msa0627090:intron 40.0%
! CATGGTGATGATAATGAAGC+TGG - chr4_3:7175493-7175512 Msa0627090:CDS 40.0%
AGAGAAAAGGAACACCTCAT+AGG - chr4_3:7175572-7175591 Msa0627090:CDS 40.0%
ACCTCATAGGCCTAAAATGT+TGG - chr4_3:7175585-7175604 Msa0627090:CDS 40.0%
! ACCAACATTTTAGGCCTATG+AGG + chr4_3:7175589-7175608 None:intergenic 40.0%
TACCTTGAAGGAAAGGAAAG+AGG + chr4_3:7175752-7175771 None:intergenic 40.0%
! CAGCAACCTTTTCTCTAGGT+AGG - chr4_3:7174249-7174268 Msa0627090:three_prime_UTR 45.0%
GTTGTTGGATCGAAATCCGA+TGG - chr4_3:7174434-7174453 Msa0627090:intron 45.0%
AGTGTAACCACACTTTCAGG+GGG + chr4_3:7174803-7174822 None:intergenic 45.0%
!! TTGAAGGCACTTAGGACTTG+AGG + chr4_3:7174872-7174891 None:intergenic 45.0%
CAAGTCCTAAGTGCCTTCAA+AGG - chr4_3:7174872-7174891 Msa0627090:intron 45.0%
!! CAGATCCTTTGAAGGCACTT+AGG + chr4_3:7174880-7174899 None:intergenic 45.0%
CTAAGTGCCTTCAAAGGATC+TGG - chr4_3:7174878-7174897 Msa0627090:intron 45.0%
! GCCTTCAAAGGATCTGGAAT+AGG - chr4_3:7174884-7174903 Msa0627090:intron 45.0%
TGCTAGGCCAAGTCTATCAA+GGG + chr4_3:7175113-7175132 None:intergenic 45.0%
TTGCTAGGCCAAGTCTATCA+AGG + chr4_3:7175114-7175133 None:intergenic 45.0%
TGGCCTAGCAAAGCAAATTG+AGG - chr4_3:7175123-7175142 Msa0627090:intron 45.0%
! CCAAAGTGTCTTTCTGATCC+TGG + chr4_3:7175658-7175677 None:intergenic 45.0%
CCAGGATCAGAAAGACACTT+TGG - chr4_3:7175655-7175674 Msa0627090:CDS 45.0%
CTTGAAGGAAAGGAAAGAGG+AGG + chr4_3:7175749-7175768 None:intergenic 45.0%
CTCCTCTTTCCTTTCCTTCA+AGG - chr4_3:7175747-7175766 Msa0627090:CDS 45.0%
! GAGAATTTGAAGCCTGGACC+AGG - chr4_3:7175637-7175656 Msa0627090:CDS 50.0%
TGTCTTTCTGATCCTGGTCC+AGG + chr4_3:7175652-7175671 None:intergenic 50.0%
AAAGAGGAGGTTGCTGAGGA+AGG + chr4_3:7175736-7175755 None:intergenic 50.0%
AAGGAAAGAGGAGGTTGCTG+AGG + chr4_3:7175740-7175759 None:intergenic 50.0%
TCTACCTCCCCCTGAAAGTG+TGG - chr4_3:7174793-7174812 Msa0627090:intron 55.0%
GTAACCACACTTTCAGGGGG+AGG + chr4_3:7174800-7174819 None:intergenic 55.0%
GCGAAGATCGAGCCATGAAC+TGG - chr4_3:7174954-7174973 Msa0627090:intron 55.0%
! GAGACTGGTTGGGCCTCTCA+TGG - chr4_3:7175475-7175494 Msa0627090:intron 60.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 7174156 7177022 7174156 ID=Msa0627090;Name=Msa0627090
chr4_3 mRNA 7174156 7177022 7174156 ID=Msa0627090-mRNA-1;Parent=Msa0627090;Name=Msa0627090-mRNA-1;_AED=0.04;_eAED=0.04;_QI=23|0.5|0.66|1|0.5|0.66|3|146|390
chr4_3 exon 7174156 7174420 7174156 ID=Msa0627090-mRNA-1:exon:2091;Parent=Msa0627090-mRNA-1
chr4_3 exon 7175481 7176446 7175481 ID=Msa0627090-mRNA-1:exon:2090;Parent=Msa0627090-mRNA-1
chr4_3 exon 7176912 7177022 7176912 ID=Msa0627090-mRNA-1:exon:2089;Parent=Msa0627090-mRNA-1
chr4_3 five_prime_UTR 7177000 7177022 7177000 ID=Msa0627090-mRNA-1:five_prime_utr;Parent=Msa0627090-mRNA-1
chr4_3 CDS 7176912 7176999 7176912 ID=Msa0627090-mRNA-1:cds;Parent=Msa0627090-mRNA-1
chr4_3 CDS 7175481 7176446 7175481 ID=Msa0627090-mRNA-1:cds;Parent=Msa0627090-mRNA-1
chr4_3 CDS 7174302 7174420 7174302 ID=Msa0627090-mRNA-1:cds;Parent=Msa0627090-mRNA-1
chr4_3 three_prime_UTR 7174156 7174301 7174156 ID=Msa0627090-mRNA-1:three_prime_utr;Parent=Msa0627090-mRNA-1
Gene Sequence

>Msa0627090

ATGCTAAGACTTCAACATCAAATGGTTCTCTCTTCCTCGTGTTCATTCTTTCTTTGGCTTCTCATTTTATCAGCAACCTTTTCTCTAGTATTAGGGAACAAAGCATTTACAGGAACATATGGAGTAAACTATGGTAGAGTAGCTGATAATCTACCTCCCCCTGAAAGTGTGGTTACACTTCTCAAAGCTGCAAAAATCAAGAATGTAAGAATCTATGATGTTAATCCTCAAGTCCTAAGTGCCTTCAAAGGATCTGGAATAGGACTTAGTGTTTGTCTTCCGAACGAACTTTTGACAGACATAGGTGTAGGCGAAGATCGAGCCATGAACTGGATTAAAGACAATGTTCAACCATATCTTCCAGGAACAAAAATAGTTGGAATTGCCATTGGAAATGAAATTTTAGGTGGTGGAAATATTGAAGTTTGGGAAGCTTTACTTCCTGCTGCAAAAAATATCTATAGTGCCCTTGATAGACTTGGCCTAGCAAAGCAAATTGAGGTTTCAACTCCACATTCAGAAGCTGTATTTGCTAATTCATATCCACCATCTTCATGTACATTTAGGGATGATATAGTACCTTACATGAAACCTTTGTTGGAATTTTTTTCACAAATTGGAACACCTTTTTATATAAATGCATACCCTTTTTTGGCCTATAAAAATGACCCTCAACATATTGACATAAACTATGCTTTATTTAAGAAAAACCCTGGCATTTATGATGCCAAAAGTAAACTACATTATGACAATATGTTTGATGCACAAGTTGATGCTGCTTATTTTGCATTGGAGAAATTTGGGTTTGATAAGATGGAAGTGATTGTTTCTGAGACTGGTTGGGCCTCTCATGGTGATGATAATGAAGCTGGAGCAACTGTGAAAAATGCAAAGACCTATAATAAAAATTTGAGAAAGAGGTTACTTAAGAGAAAAGGAACACCTCATAGGCCTAAAATGTTGGTTAGGGTTTATATATTTGCTTTGTTCAATGAGAATTTGAAGCCTGGACCAGGATCAGAAAGACACTTTGGATTATTTAATCATGATGGAAGGAATTGGGTCTTCATACATGATGGTTTCTTCAAGTTGTGTTGCAATTCTTCTTCTTCTTATAGCATTGTAATCAGTCAAAAGATTGTAGATACAAGAACTGAGTTAACGATTCTTTGA
Protein sequence

>Msa0627090

MLRLQHQMVLSSSCSFFLWLLILSATFSLVLGNKAFTGTYGVNYGRVADNLPPPESVVTLLKAAKIKNVRIYDVNPQVLSAFKGSGIGLSVCLPNELLTDIGVGEDRAMNWIKDNVQPYLPGTKIVGIAIGNEILGGGNIEVWEALLPAAKNIYSALDRLGLAKQIEVSTPHSEAVFANSYPPSSCTFRDDIVPYMKPLLEFFSQIGTPFYINAYPFLAYKNDPQHIDINYALFKKNPGIYDAKSKLHYDNMFDAQVDAAYFALEKFGFDKMEVIVSETGWASHGDDNEAGATVKNAKTYNKNLRKRLLKRKGTPHRPKMLVRVYIFALFNENLKPGPGSERHFGLFNHDGRNWVFIHDGFFKLCCNSSSSYSIVISQKIVDTRTELTIL*