AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0819730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0819730 MtrunA17_Chr5g0439101 100.000 190 0 0 1 190 1 190 2.81e-141 391
Msa0819730 MtrunA17_Chr3g0119621 92.063 189 15 0 1 189 1 189 2.73e-132 368
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0819730 AT3G47810.2 87.634 186 23 0 1 186 1 186 1.33e-123 347
Msa0819730 AT3G47810.1 87.634 186 23 0 1 186 1 186 1.33e-123 347
Msa0819730 AT3G47810.3 87.634 186 23 0 1 186 1 186 1.33e-123 347

Find 91 sgRNAs with CRISPR-Local

Find 262 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGGTGTTGGTCTTGGCCC+TGG 0.225620 5_3:+56480649 Msa0819730:exon
CTAGACCAATCACTGGTAAA+AGG 0.225755 5_3:-56482823 None:intergenic
AAGTTTGAAACACATGTATT+TGG 0.238943 5_3:+56482726 Msa0819730:three_prime_UTR
GGCATAATCCCAACTTAAAT+TGG 0.245475 5_3:-56481791 None:intergenic
GAGAGAGAAACCTTTGTTAA+TGG 0.266325 5_3:-56479084 None:intergenic
CATGTATTTGGCAATATCTT+AGG 0.269228 5_3:+56482738 Msa0819730:three_prime_UTR
ATCAATTTCACTAATTCTTT+AGG 0.276148 5_3:-56478965 None:intergenic
TTGAACTTTGCAGGAATATC+AGG 0.293631 5_3:-56480698 None:intergenic
ATGGTGTTGGTCTTGGCCCT+GGG 0.308621 5_3:+56480650 Msa0819730:CDS
AGCATGGATTTGAACTTTGC+AGG 0.319977 5_3:-56480707 None:intergenic
GCCGCTCTCCTCCCGGTGTT+TGG 0.324799 5_3:+56479004 Msa0819730:five_prime_UTR
TCTCTCCTTCAAAGTTCATA+TGG 0.346590 5_3:+56482687 Msa0819730:three_prime_UTR
ACTTATTGATGGAGAGGTTA+AGG 0.377877 5_3:+56482552 Msa0819730:CDS
AACTATTGGCCAATTTAAGT+TGG 0.382873 5_3:+56481782 Msa0819730:CDS
ATAGTTAGTGTCTTGGTCTC+TGG 0.388935 5_3:-56481765 None:intergenic
GGGATTATGCCATGGTCATC+AGG 0.390392 5_3:+56481803 Msa0819730:CDS
TCACTCGACCGATCGGAAGC+TGG 0.391561 5_3:-56479042 None:intergenic
ACCACGAGTTATATGCAAAT+CGG 0.409593 5_3:-56481718 None:intergenic
ATTGAGGATGGTGTTGGTCT+TGG 0.410041 5_3:+56480643 Msa0819730:exon
CAGGAATATCAGGCGCCCTA+TGG 0.417399 5_3:-56480688 None:intergenic
TAGACCAATCACTGGTAAAA+GGG 0.423718 5_3:-56482822 None:intergenic
AATCGGGACAAAGAGTCTTT+AGG 0.427225 5_3:-56481701 None:intergenic
ATAAATCCAGGTTCTGCATC+TGG 0.431341 5_3:+56482442 Msa0819730:CDS
GACCGAAGTGTTCAAAGAGC+AGG 0.437744 5_3:-56482613 None:intergenic
ACTATTGGCCAATTTAAGTT+GGG 0.447997 5_3:+56481783 Msa0819730:CDS
TATGTATATGAACTTATTGA+TGG 0.449262 5_3:+56482541 Msa0819730:CDS
AACATCCATATGAACTTTGA+AGG 0.457048 5_3:-56482692 None:intergenic
AGGAATATCAGGCGCCCTAT+GGG 0.464637 5_3:-56480687 None:intergenic
AAAACTTGGGTTCACGTCAT+AGG 0.469045 5_3:-56482482 None:intergenic
CACTCGACCGATCGGAAGCT+GGG 0.469220 5_3:-56479041 None:intergenic
AAATTGATCTGACGTGGAAT+TGG 0.473150 5_3:+56478980 Msa0819730:five_prime_UTR
AATTGGTGCCGCTCTCCTCC+CGG 0.473202 5_3:+56478997 Msa0819730:five_prime_UTR
ATCCAGCACATCATTTGTAC+TGG 0.480280 5_3:+56480743 Msa0819730:CDS
TAGGCACCAGATGCAGAACC+TGG 0.486891 5_3:-56482448 None:intergenic
TTCCAGTACAAATGATGTGC+TGG 0.495301 5_3:-56480745 None:intergenic
TTGGCCAATAGTTAGTGTCT+TGG 0.497184 5_3:-56481772 None:intergenic
TTCAAATCCATGCTTGTCCC+TGG 0.497945 5_3:+56480716 Msa0819730:CDS
GAGACCAAGACACTAACTAT+TGG 0.502451 5_3:+56481768 Msa0819730:CDS
ATGATGTGCTGGATCTTGCC+AGG 0.502900 5_3:-56480734 None:intergenic
CCACGAGTTATATGCAAATC+GGG 0.503756 5_3:-56481717 None:intergenic
ACTAAACAACCAAATAGAAA+AGG 0.504053 5_3:-56482655 None:intergenic
ATGTCCATAAGCACAAAACT+TGG 0.507271 5_3:-56482496 None:intergenic
TTTGATATTGAGGATGGTGT+TGG 0.515815 5_3:+56480637 Msa0819730:exon
AGAGTTGTCACTCGACCGAT+CGG 0.528608 5_3:-56479049 None:intergenic
GGTGGTGTGGTTATAAATCC+AGG 0.536057 5_3:+56482430 Msa0819730:CDS
AAAGAGTCTTTAGGTAGTCA+TGG 0.540944 5_3:-56481692 None:intergenic
TGGTGTTGGTCTTGGCCCTG+GGG 0.544238 5_3:+56480651 Msa0819730:CDS
GGTGTTGGTCTTGGCCCTGG+GGG 0.546940 5_3:+56480652 Msa0819730:CDS
GTAGCATCGCAAGTGAGTCT+AGG 0.549473 5_3:-56482339 None:intergenic
ACCAAACACCGGGAGGAGAG+CGG 0.563102 5_3:-56479005 None:intergenic
CTCCGCTCCGCCATTAACAA+AGG 0.565994 5_3:+56479074 Msa0819730:five_prime_UTR
TTTAAGTTGGGATTATGCCA+TGG 0.567941 5_3:+56481795 Msa0819730:CDS
ACCGAAGTGTTCAAAGAGCA+GGG 0.577116 5_3:-56482612 None:intergenic
GATTGTGAAGGTTATTCCAT+GGG 0.581252 5_3:+56482312 Msa0819730:intron
CCCGATTTGCATATAACTCG+TGG 0.581414 5_3:+56481717 Msa0819730:CDS
CATTGATGCTCTGCGTGTCG+TGG 0.583073 5_3:+56482516 Msa0819730:CDS
CTTATGTGACTGATTAGCAA+TGG 0.585053 5_3:+56482789 Msa0819730:three_prime_UTR
GATGTAGACATCCTTGTCAC+AGG 0.586446 5_3:+56482373 Msa0819730:CDS
GTTTGTAAGCAGTAAACTGA+TGG 0.593009 5_3:-56482402 None:intergenic
GGGATATGCACATACCCCAT+AGG 0.593817 5_3:+56480672 Msa0819730:CDS
GTTTACTGCTTACAAACATG+AGG 0.595798 5_3:+56482408 Msa0819730:CDS
TGTCCATAAGCACAAAACTT+GGG 0.598526 5_3:-56482495 None:intergenic
GTCATAGGTCATGCTGCTGT+AGG 0.598582 5_3:-56482467 None:intergenic
TCCCTGCTCTTTGAACACTT+CGG 0.599055 5_3:+56482611 Msa0819730:exon
GGATATGCACATACCCCATA+GGG 0.605484 5_3:+56480673 Msa0819730:CDS
ATATGAACTTATTGATGGAG+AGG 0.605775 5_3:+56482546 Msa0819730:CDS
GATCTTGCCAGGGACAAGCA+TGG 0.611475 5_3:-56480723 None:intergenic
AGACCAATCACTGGTAAAAG+GGG 0.616248 5_3:-56482821 None:intergenic
AAGATCTCTAGACCAATCAC+TGG 0.617159 5_3:-56482830 None:intergenic
TGATTGTGAAGGTTATTCCA+TGG 0.617698 5_3:+56482311 Msa0819730:intron
AGAGAAACCTTTGTTAATGG+CGG 0.619481 5_3:-56479081 None:intergenic
TCTACCACCCAGCTTCCGAT+CGG 0.624242 5_3:+56479034 Msa0819730:five_prime_UTR
AAAATCAAACCTGATGACCA+TGG 0.638503 5_3:-56481812 None:intergenic
ATTTAACAAGGTGAAGATCG+TGG 0.640848 5_3:-56482884 None:intergenic
AACCTTTGTTAATGGCGGAG+CGG 0.655481 5_3:-56479076 None:intergenic
TTTACTGCTTACAAACATGA+GGG 0.656484 5_3:+56482409 Msa0819730:CDS
TAGAATCAAAACCAAACACC+GGG 0.657239 5_3:-56479015 None:intergenic
GTAGAATCAAAACCAAACAC+CGG 0.658578 5_3:-56479016 None:intergenic
AGTGAGTCTAGGTCTCCCCA+TGG 0.660534 5_3:-56482328 None:intergenic
TTAGTGAAATTGATCTGACG+TGG 0.660563 5_3:+56478974 Msa0819730:five_prime_UTR
TGATGTGCTGGATCTTGCCA+GGG 0.662880 5_3:-56480733 None:intergenic
ACTAATGCTAACATTTAACA+AGG 0.672810 5_3:-56482896 None:intergenic
GGAATATCAGGCGCCCTATG+GGG 0.674568 5_3:-56480686 None:intergenic
ATTGTGAAGGTTATTCCATG+GGG 0.683679 5_3:+56482313 Msa0819730:intron
TTTGTAAGCAGTAAACTGAT+GGG 0.684054 5_3:-56482401 None:intergenic
ACTGCTTACAAACATGAGGG+TGG 0.696775 5_3:+56482412 Msa0819730:CDS
TGATAAATTTGTGATCTGTG+AGG 0.697738 5_3:+56482764 Msa0819730:three_prime_UTR
TTACAAACATGAGGGTGGTG+TGG 0.697908 5_3:+56482417 Msa0819730:CDS
AATCAAAACCAAACACCGGG+AGG 0.706164 5_3:-56479012 None:intergenic
GATGGGTGTGACCTGTGACA+AGG 0.707385 5_3:-56482384 None:intergenic
TCGACCGATCGGAAGCTGGG+TGG 0.708948 5_3:-56479038 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGAATTTGAAATTAATTTAG+AGG + chr5_3:56479422-56479441 Msa0819730:intron 15.0%
!!! TCATTTTCTCAAATTATTAT+TGG + chr5_3:56479562-56479581 Msa0819730:intron 15.0%
!!! ATTTCAAATTTTCAATAAGA+TGG - chr5_3:56480791-56480810 None:intergenic 15.0%
!!! TAATTCAGTAAATTTTGTTA+TGG + chr5_3:56481559-56481578 Msa0819730:intron 15.0%
!!! ACTTTTTAAGAAACTATTAT+TGG + chr5_3:56482224-56482243 Msa0819730:intron 15.0%
!!! TATATATCATACACATTTTT+TGG - chr5_3:56482266-56482285 None:intergenic 15.0%
!! ATCAATTTCACTAATTCTTT+AGG - chr5_3:56478968-56478987 None:intergenic 20.0%
!! ACATACAGTATAATAAAAAC+AGG - chr5_3:56479172-56479191 None:intergenic 20.0%
!!! CAATGATTTTAAACTTTTGA+AGG - chr5_3:56479331-56479350 None:intergenic 20.0%
!!! AAATTATTATTGGTGTCAAT+GGG + chr5_3:56479572-56479591 Msa0819730:intron 20.0%
!!! TTTAATTTGGTGCTTATTTA+TGG + chr5_3:56479912-56479931 Msa0819730:intron 20.0%
!!! TTTGCTTTGGTTTTTTTAAA+AGG + chr5_3:56480093-56480112 Msa0819730:intron 20.0%
!!! TTGCTTTGGTTTTTTTAAAA+GGG + chr5_3:56480094-56480113 Msa0819730:intron 20.0%
!! GAATCAAATATTCAAAATAG+AGG + chr5_3:56480262-56480281 Msa0819730:intron 20.0%
!!! ATATTCAAAATAGAGGTTTT+TGG + chr5_3:56480269-56480288 Msa0819730:intron 20.0%
!! TTGTAGAATATGAAATCATT+AGG + chr5_3:56481037-56481056 Msa0819730:intron 20.0%
!! TATTAAACAACTAGTAAAGA+TGG + chr5_3:56481201-56481220 Msa0819730:intron 20.0%
!! ATTAAACAACTAGTAAAGAT+GGG + chr5_3:56481202-56481221 Msa0819730:intron 20.0%
!! AAATTATAGTTAATGGGAAT+AGG - chr5_3:56481612-56481631 None:intergenic 20.0%
!! GCTTTCAAATTATAGTTAAT+GGG - chr5_3:56481618-56481637 None:intergenic 20.0%
!! TGCTTTCAAATTATAGTTAA+TGG - chr5_3:56481619-56481638 None:intergenic 20.0%
!!! GTTTTATGTCATTTATATTC+AGG + chr5_3:56481665-56481684 Msa0819730:intron 20.0%
!!! GTTTGATTTTATTTTTACTC+TGG + chr5_3:56481825-56481844 Msa0819730:intron 20.0%
!! TATGTATATGAACTTATTGA+TGG + chr5_3:56482541-56482560 Msa0819730:CDS 20.0%
!!! TTTATCTTTCCTTTTCTATT+TGG + chr5_3:56482646-56482665 Msa0819730:three_prime_UTR 20.0%
!!! AGGTTTTGAGATTAGTAATT+AGG + chr5_3:56479442-56479461 Msa0819730:intron 25.0%
!!! CAAATTATTATTGGTGTCAA+TGG + chr5_3:56479571-56479590 Msa0819730:intron 25.0%
!!! TATCATATTTGTTGTTAGCA+AGG + chr5_3:56479630-56479649 Msa0819730:intron 25.0%
!! GAAGTGTTTGATAAAATTAG+CGG - chr5_3:56480442-56480461 None:intergenic 25.0%
!! TTTTTAATAGAAGACAAGAG+AGG - chr5_3:56480522-56480541 None:intergenic 25.0%
!! ACTTTCAGTTTTTGATATTG+AGG + chr5_3:56480627-56480646 Msa0819730:five_prime_UTR 25.0%
!! TAACTATTACTGATCCATTT+TGG + chr5_3:56480810-56480829 Msa0819730:intron 25.0%
! GAATATGAAATCATTAGGAA+AGG + chr5_3:56481042-56481061 Msa0819730:intron 25.0%
! ATAAAGGAGTTAAATGATGA+TGG + chr5_3:56481264-56481283 Msa0819730:intron 25.0%
!!! TATTTTGATGAGAGAATGAT+TGG - chr5_3:56481353-56481372 None:intergenic 25.0%
!! TGGTTTTTATGCCTTTTTTT+TGG + chr5_3:56481845-56481864 Msa0819730:intron 25.0%
!!! AATCTCTAGAACCAAAAAAA+AGG - chr5_3:56481859-56481878 None:intergenic 25.0%
!!! TTTTTTGGTTCTAGAGATTA+TGG + chr5_3:56481860-56481879 Msa0819730:intron 25.0%
!!! TCTGTTTTATTTTATTGTCC+TGG + chr5_3:56482153-56482172 Msa0819730:intron 25.0%
! TTCTTAAAAAGTTCAATCAC+TGG - chr5_3:56482216-56482235 None:intergenic 25.0%
! ACTAAACAACCAAATAGAAA+AGG - chr5_3:56482658-56482677 None:intergenic 25.0%
!! AAGTTTGAAACACATGTATT+TGG + chr5_3:56482726-56482745 Msa0819730:three_prime_UTR 25.0%
GGAATTGAAGAAGTAAGAAA+CGG - chr5_3:56479151-56479170 None:intergenic 30.0%
AGTTGTGACTAAACATCTAT+CGG - chr5_3:56479386-56479405 None:intergenic 30.0%
TTGAGATTAGTAATTAGGAG+AGG + chr5_3:56479447-56479466 Msa0819730:intron 30.0%
TAGGAGAGGAAATACTATTT+AGG + chr5_3:56479461-56479480 Msa0819730:intron 30.0%
AGGAGAGGAAATACTATTTA+GGG + chr5_3:56479462-56479481 Msa0819730:intron 30.0%
! CATTGACTATGAATGTTGAA+TGG + chr5_3:56479745-56479764 Msa0819730:intron 30.0%
AATGGAGGATATATAAGAGA+GGG + chr5_3:56479763-56479782 Msa0819730:intron 30.0%
!! TATCAAGGCATTGAGAATAT+GGG - chr5_3:56479791-56479810 None:intergenic 30.0%
!!! ACACGATGTGTGTTTTAATT+TGG + chr5_3:56479899-56479918 Msa0819730:intron 30.0%
!! ATTTGGTGCTTATTTATGGA+TGG + chr5_3:56479916-56479935 Msa0819730:intron 30.0%
! TTTGATGTTAGCTTAACTCT+AGG + chr5_3:56479977-56479996 Msa0819730:intron 30.0%
! GCAAAATCACTAGCTTATAT+TGG + chr5_3:56480228-56480247 Msa0819730:intron 30.0%
GAAACACACTATAATCTAGT+GGG - chr5_3:56480308-56480327 None:intergenic 30.0%
TGAAACACACTATAATCTAG+TGG - chr5_3:56480309-56480328 None:intergenic 30.0%
TATAGCAGATGAACTTATAG+AGG - chr5_3:56480486-56480505 None:intergenic 30.0%
! TCGCTGTTCTTTTTGTTAAT+AGG + chr5_3:56480572-56480591 Msa0819730:intron 30.0%
! TCAGTTTTTGATATTGAGGA+TGG + chr5_3:56480631-56480650 Msa0819730:exon 30.0%
CACATAAGGTTCAAAAGTTT+CGG - chr5_3:56480906-56480925 None:intergenic 30.0%
! GAAACTTTTGAACCTTATGT+GGG + chr5_3:56480905-56480924 Msa0819730:intron 30.0%
! AAACTTTTGAACCTTATGTG+GGG + chr5_3:56480906-56480925 Msa0819730:intron 30.0%
GTACTTGAAAATTTAGCTGT+TGG + chr5_3:56481088-56481107 Msa0819730:intron 30.0%
! TGTGCTATTGATGACAATAA+AGG + chr5_3:56481248-56481267 Msa0819730:intron 30.0%
! ACATTGAGTTTTTGTGACAT+TGG - chr5_3:56481409-56481428 None:intergenic 30.0%
TGGAACAAACGGAAAATAAT+TGG - chr5_3:56481435-56481454 None:intergenic 30.0%
CAAAAGGAGTTAATATAGCT+TGG - chr5_3:56481504-56481523 None:intergenic 30.0%
GATAATTGGTGCAATACAAA+AGG - chr5_3:56481520-56481539 None:intergenic 30.0%
AACTATTGGCCAATTTAAGT+TGG + chr5_3:56481782-56481801 Msa0819730:CDS 30.0%
! ACTATTGGCCAATTTAAGTT+GGG + chr5_3:56481783-56481802 Msa0819730:CDS 30.0%
TGCAATCTAAACTAACAGAA+TGG - chr5_3:56482043-56482062 None:intergenic 30.0%
AGTTTAGATTGCACTTGTAT+AGG + chr5_3:56482050-56482069 Msa0819730:intron 30.0%
ACAATGCAAAAACATCAACA+AGG - chr5_3:56482105-56482124 None:intergenic 30.0%
CATTGTCAGTAATAGCTAAT+AGG + chr5_3:56482119-56482138 Msa0819730:intron 30.0%
TTAAAAAGTTCAATCACTGG+TGG - chr5_3:56482213-56482232 None:intergenic 30.0%
! TTATGTCTTGATGATTGTGA+AGG + chr5_3:56482300-56482319 Msa0819730:intron 30.0%
TTTGTAAGCAGTAAACTGAT+GGG - chr5_3:56482404-56482423 None:intergenic 30.0%
TTTACTGCTTACAAACATGA+GGG + chr5_3:56482409-56482428 Msa0819730:CDS 30.0%
ATATGAACTTATTGATGGAG+AGG + chr5_3:56482546-56482565 Msa0819730:CDS 30.0%
AACATCCATATGAACTTTGA+AGG - chr5_3:56482695-56482714 None:intergenic 30.0%
CATGTATTTGGCAATATCTT+AGG + chr5_3:56482738-56482757 Msa0819730:three_prime_UTR 30.0%
TGATAAATTTGTGATCTGTG+AGG + chr5_3:56482764-56482783 Msa0819730:three_prime_UTR 30.0%
TTAGTGAAATTGATCTGACG+TGG + chr5_3:56478974-56478993 Msa0819730:five_prime_UTR 35.0%
AAATTGATCTGACGTGGAAT+TGG + chr5_3:56478980-56478999 Msa0819730:five_prime_UTR 35.0%
TAGAATCAAAACCAAACACC+GGG - chr5_3:56479018-56479037 None:intergenic 35.0%
GTAGAATCAAAACCAAACAC+CGG - chr5_3:56479019-56479038 None:intergenic 35.0%
AGAGAAACCTTTGTTAATGG+CGG - chr5_3:56479084-56479103 None:intergenic 35.0%
GAGAGAGAAACCTTTGTTAA+TGG - chr5_3:56479087-56479106 None:intergenic 35.0%
! CGAATCAAAAACTGATTCAG+TGG - chr5_3:56479251-56479270 None:intergenic 35.0%
TTTCTCAGAAATTCTGCGTT+TGG + chr5_3:56479276-56479295 Msa0819730:intron 35.0%
GGAGAGGAAATACTATTTAG+GGG + chr5_3:56479463-56479482 Msa0819730:intron 35.0%
TACTAGTCAACTATGAAGCA+CGG - chr5_3:56479612-56479631 None:intergenic 35.0%
TTTCCCACATCACTATATGT+GGG - chr5_3:56479681-56479700 None:intergenic 35.0%
! GAGATTATTGAAAGTGTGAG+TGG + chr5_3:56479713-56479732 Msa0819730:intron 35.0%
TGACTATGAATGTTGAATGG+AGG + chr5_3:56479748-56479767 Msa0819730:intron 35.0%
GAATGGAGGATATATAAGAG+AGG + chr5_3:56479762-56479781 Msa0819730:intron 35.0%
ATGGAGGATATATAAGAGAG+GGG + chr5_3:56479764-56479783 Msa0819730:intron 35.0%
!! GTATCAAGGCATTGAGAATA+TGG - chr5_3:56479792-56479811 None:intergenic 35.0%
GCTTATTTATGGATGGAGAT+TGG + chr5_3:56479923-56479942 Msa0819730:intron 35.0%
TAATCTTCTTAGCCACCTTA+TGG + chr5_3:56480006-56480025 Msa0819730:intron 35.0%
ATTATCCGAATACCATAAGG+TGG - chr5_3:56480021-56480040 None:intergenic 35.0%
ACCTTATGGTATTCGGATAA+TGG + chr5_3:56480020-56480039 Msa0819730:intron 35.0%
ACCATTATCCGAATACCATA+AGG - chr5_3:56480024-56480043 None:intergenic 35.0%
! GGATAATGGTTATACCAAAG+TGG + chr5_3:56480034-56480053 Msa0819730:intron 35.0%
TTTCCTTGTTGGGAATTTGT+AGG + chr5_3:56480057-56480076 Msa0819730:intron 35.0%
!! TGCTTTGTTAATTAGCTTGC+TGG + chr5_3:56480124-56480143 Msa0819730:intron 35.0%
!! GCTTTGTTAATTAGCTTGCT+GGG + chr5_3:56480125-56480144 Msa0819730:intron 35.0%
!! CTTTGTTAATTAGCTTGCTG+GGG + chr5_3:56480126-56480145 Msa0819730:intron 35.0%
TCAAGTAGATGTCTTATCCT+TGG + chr5_3:56480180-56480199 Msa0819730:intron 35.0%
! TCTTATCCTTGGTTCTCATA+CGG + chr5_3:56480191-56480210 Msa0819730:intron 35.0%
! AGGAAATACTTTGTTTGTGC+AGG + chr5_3:56480592-56480611 Msa0819730:intron 35.0%
TTTGATATTGAGGATGGTGT+TGG + chr5_3:56480637-56480656 Msa0819730:exon 35.0%
TTGAACTTTGCAGGAATATC+AGG - chr5_3:56480701-56480720 None:intergenic 35.0%
ATTCAGACTTAGCATTAGCA+TGG - chr5_3:56480842-56480861 None:intergenic 35.0%
! CGAAACTTTTGAACCTTATG+TGG + chr5_3:56480904-56480923 Msa0819730:intron 35.0%
! AGTTTTCTACCTAAGAAGCT+TGG - chr5_3:56481175-56481194 None:intergenic 35.0%
AAGCTTCTTAGGTAGAAAAC+TGG + chr5_3:56481174-56481193 Msa0819730:intron 35.0%
!! AGGAGTTAAATGATGATGGT+AGG + chr5_3:56481268-56481287 Msa0819730:intron 35.0%
!! TAAATGATGATGGTAGGTTG+TGG + chr5_3:56481274-56481293 Msa0819730:intron 35.0%
!! AAATGATGATGGTAGGTTGT+GGG + chr5_3:56481275-56481294 Msa0819730:intron 35.0%
! TGATGAGAGAATGATTGGTT+AGG - chr5_3:56481348-56481367 None:intergenic 35.0%
AAAGAGTCTTTAGGTAGTCA+TGG - chr5_3:56481695-56481714 None:intergenic 35.0%
ACCACGAGTTATATGCAAAT+CGG - chr5_3:56481721-56481740 None:intergenic 35.0%
GGCATAATCCCAACTTAAAT+TGG - chr5_3:56481794-56481813 None:intergenic 35.0%
!! TTTAAGTTGGGATTATGCCA+TGG + chr5_3:56481795-56481814 Msa0819730:CDS 35.0%
AAAATCAAACCTGATGACCA+TGG - chr5_3:56481815-56481834 None:intergenic 35.0%
! ACTTTGGGTTTCATGATCTA+AGG + chr5_3:56481910-56481929 Msa0819730:intron 35.0%
! GTTTCATGATCTAAGGTCTA+TGG + chr5_3:56481917-56481936 Msa0819730:intron 35.0%
!! TTGACATTGATGATGTAGCA+GGG - chr5_3:56482007-56482026 None:intergenic 35.0%
!! TTTGACATTGATGATGTAGC+AGG - chr5_3:56482008-56482027 None:intergenic 35.0%
ACATCAACAAGGAAGAAGAT+CGG - chr5_3:56482094-56482113 None:intergenic 35.0%
GATCGTTGAATGGAAACATT+AGG - chr5_3:56482181-56482200 None:intergenic 35.0%
TGATTGTGAAGGTTATTCCA+TGG + chr5_3:56482311-56482330 Msa0819730:intron 35.0%
GATTGTGAAGGTTATTCCAT+GGG + chr5_3:56482312-56482331 Msa0819730:intron 35.0%
ATTGTGAAGGTTATTCCATG+GGG + chr5_3:56482313-56482332 Msa0819730:intron 35.0%
GTTTGTAAGCAGTAAACTGA+TGG - chr5_3:56482405-56482424 None:intergenic 35.0%
GTTTACTGCTTACAAACATG+AGG + chr5_3:56482408-56482427 Msa0819730:CDS 35.0%
TGTCCATAAGCACAAAACTT+GGG - chr5_3:56482498-56482517 None:intergenic 35.0%
ATGTCCATAAGCACAAAACT+TGG - chr5_3:56482499-56482518 None:intergenic 35.0%
ACTTATTGATGGAGAGGTTA+AGG + chr5_3:56482552-56482571 Msa0819730:CDS 35.0%
TCTCTCCTTCAAAGTTCATA+TGG + chr5_3:56482687-56482706 Msa0819730:three_prime_UTR 35.0%
CTTATGTGACTGATTAGCAA+TGG + chr5_3:56482789-56482808 Msa0819730:three_prime_UTR 35.0%
TAGACCAATCACTGGTAAAA+GGG - chr5_3:56482825-56482844 None:intergenic 35.0%
ATTTAACAAGGTGAAGATCG+TGG - chr5_3:56482887-56482906 None:intergenic 35.0%
! ATTTAGGGGTGCACAATCTT+TGG + chr5_3:56479477-56479496 Msa0819730:intron 40.0%
TAATCACATGTGTCAGACAC+CGG - chr5_3:56479530-56479549 None:intergenic 40.0%
TAGTCCCACATATAGTGATG+TGG + chr5_3:56479674-56479693 Msa0819730:intron 40.0%
AGTCCCACATATAGTGATGT+GGG + chr5_3:56479675-56479694 Msa0819730:intron 40.0%
GTTTCCCACATCACTATATG+TGG - chr5_3:56479682-56479701 None:intergenic 40.0%
AGTGATGTGGGAAACTCAAT+TGG + chr5_3:56479687-56479706 Msa0819730:intron 40.0%
GTGATGTGGGAAACTCAATT+GGG + chr5_3:56479688-56479707 Msa0819730:intron 40.0%
TGATGTGGGAAACTCAATTG+GGG + chr5_3:56479689-56479708 Msa0819730:intron 40.0%
TGGAGGATATATAAGAGAGG+GGG + chr5_3:56479765-56479784 Msa0819730:intron 40.0%
CAATGCCTTGATACTTTGAG+TGG + chr5_3:56479798-56479817 Msa0819730:intron 40.0%
! GATCTCCACTCAAAGTATCA+AGG - chr5_3:56479806-56479825 None:intergenic 40.0%
TACTTTGAGTGGAGATCATG+TGG + chr5_3:56479809-56479828 Msa0819730:intron 40.0%
TTTATGGATGGAGATTGGAG+AGG + chr5_3:56479928-56479947 Msa0819730:intron 40.0%
CTTAGCCACCTTATGGTATT+CGG + chr5_3:56480013-56480032 Msa0819730:intron 40.0%
TACCAAAGTGGTTTCCTTGT+TGG + chr5_3:56480046-56480065 Msa0819730:intron 40.0%
ACCAAAGTGGTTTCCTTGTT+GGG + chr5_3:56480047-56480066 Msa0819730:intron 40.0%
CTTCCTACAAATTCCCAACA+AGG - chr5_3:56480063-56480082 None:intergenic 40.0%
TTGGGAATTTGTAGGAAGAC+TGG + chr5_3:56480065-56480084 Msa0819730:intron 40.0%
!!! TAGCTTGCTGGGGTTATTTT+AGG + chr5_3:56480136-56480155 Msa0819730:intron 40.0%
ACACTACTACAAGTTGTGTC+TGG - chr5_3:56480349-56480368 None:intergenic 40.0%
AGCATGGATTTGAACTTTGC+AGG - chr5_3:56480710-56480729 None:intergenic 40.0%
ATCCAGCACATCATTTGTAC+TGG + chr5_3:56480743-56480762 Msa0819730:CDS 40.0%
TTCCAGTACAAATGATGTGC+TGG - chr5_3:56480748-56480767 None:intergenic 40.0%
TAGCATGGTAACTGCCAAAA+TGG - chr5_3:56480827-56480846 None:intergenic 40.0%
CATATAGTCAACCCCACATA+AGG - chr5_3:56480920-56480939 None:intergenic 40.0%
GAAGCTTGGACAATGACAAA+GGG - chr5_3:56481161-56481180 None:intergenic 40.0%
AGAAGCTTGGACAATGACAA+AGG - chr5_3:56481162-56481181 None:intergenic 40.0%
TGTCATTGTCCAAGCTTCTT+AGG + chr5_3:56481163-56481182 Msa0819730:intron 40.0%
TAAACTCAATGCCACCAAAG+TGG - chr5_3:56481303-56481322 None:intergenic 40.0%
TGCAACAAAGGTGGAACAAA+CGG - chr5_3:56481446-56481465 None:intergenic 40.0%
TTTGTTCCACCTTTGTTGCA+TGG + chr5_3:56481446-56481465 Msa0819730:intron 40.0%
TTGTTCCACCTTTGTTGCAT+GGG + chr5_3:56481447-56481466 Msa0819730:intron 40.0%
TGATAGAACCCATGCAACAA+AGG - chr5_3:56481458-56481477 None:intergenic 40.0%
CGTAGATGCAGACAGATAAT+TGG - chr5_3:56481534-56481553 None:intergenic 40.0%
AATCGGGACAAAGAGTCTTT+AGG - chr5_3:56481704-56481723 None:intergenic 40.0%
CCACGAGTTATATGCAAATC+GGG - chr5_3:56481720-56481739 None:intergenic 40.0%
ATAGTTAGTGTCTTGGTCTC+TGG - chr5_3:56481768-56481787 None:intergenic 40.0%
GAGACCAAGACACTAACTAT+TGG + chr5_3:56481768-56481787 Msa0819730:CDS 40.0%
TTGGCCAATAGTTAGTGTCT+TGG - chr5_3:56481775-56481794 None:intergenic 40.0%
! TGGTTTTCTCTGTAGTTGAG+AGG + chr5_3:56481880-56481899 Msa0819730:intron 40.0%
! GGTTTTCTCTGTAGTTGAGA+GGG + chr5_3:56481881-56481900 Msa0819730:intron 40.0%
GGGCAATCTATGAGAATACT+TGG - chr5_3:56481987-56482006 None:intergenic 40.0%
GAACTTGGAAGATCGTTGAA+TGG - chr5_3:56482191-56482210 None:intergenic 40.0%
ATAAATCCAGGTTCTGCATC+TGG + chr5_3:56482442-56482461 Msa0819730:CDS 40.0%
AAAACTTGGGTTCACGTCAT+AGG - chr5_3:56482485-56482504 None:intergenic 40.0%
!! GAACCCAAGTTTTGTGCTTA+TGG + chr5_3:56482492-56482511 Msa0819730:CDS 40.0%
AGACCAATCACTGGTAAAAG+GGG - chr5_3:56482824-56482843 None:intergenic 40.0%
CTAGACCAATCACTGGTAAA+AGG - chr5_3:56482826-56482845 None:intergenic 40.0%
AAGATCTCTAGACCAATCAC+TGG - chr5_3:56482833-56482852 None:intergenic 40.0%
AATCAAAACCAAACACCGGG+AGG - chr5_3:56479015-56479034 None:intergenic 45.0%
! AACCTTTGTTAATGGCGGAG+CGG - chr5_3:56479079-56479098 None:intergenic 45.0%
GATGTGGGAAACTCAATTGG+GGG + chr5_3:56479690-56479709 Msa0819730:intron 45.0%
ATGTGGGAAACTCAATTGGG+GGG + chr5_3:56479691-56479710 Msa0819730:intron 45.0%
GCATCAACAAGCTAACAAGC+AGG - chr5_3:56479853-56479872 None:intergenic 45.0%
TCCCAACAAGGAAACCACTT+TGG - chr5_3:56480051-56480070 None:intergenic 45.0%
AAGACTGGTGAGCTTTGCTT+TGG + chr5_3:56480080-56480099 Msa0819730:intron 45.0%
AAAGAGCCGTATGAGAACCA+AGG - chr5_3:56480200-56480219 None:intergenic 45.0%
! GGTTCTCATACGGCTCTTTT+TGG + chr5_3:56480201-56480220 Msa0819730:intron 45.0%
!! TCATACGGCTCTTTTTGGCA+AGG + chr5_3:56480206-56480225 Msa0819730:intron 45.0%
!! ATTGAGGATGGTGTTGGTCT+TGG + chr5_3:56480643-56480662 Msa0819730:exon 45.0%
GGATATGCACATACCCCATA+GGG + chr5_3:56480673-56480692 Msa0819730:CDS 45.0%
TTCAAATCCATGCTTGTCCC+TGG + chr5_3:56480716-56480735 Msa0819730:CDS 45.0%
CTGCGACAACACAAAAGTTG+AGG - chr5_3:56481117-56481136 None:intergenic 45.0%
! ACAGTAGCACTGAAGGTACA+GGG - chr5_3:56481231-56481250 None:intergenic 45.0%
! CAATAGCACAGTAGCACTGA+AGG - chr5_3:56481238-56481257 None:intergenic 45.0%
TAGAACCCATGCAACAAAGG+TGG - chr5_3:56481455-56481474 None:intergenic 45.0%
! CCCGATTTGCATATAACTCG+TGG + chr5_3:56481717-56481736 Msa0819730:CDS 45.0%
!! TTGAGAGGGTGCTTCACTTT+GGG + chr5_3:56481895-56481914 Msa0819730:intron 45.0%
GAATGGAAACATTAGGAGCC+AGG - chr5_3:56482174-56482193 None:intergenic 45.0%
GTTCAATCACTGGTGGAACT+TGG - chr5_3:56482206-56482225 None:intergenic 45.0%
GATGTAGACATCCTTGTCAC+AGG + chr5_3:56482373-56482392 Msa0819730:CDS 45.0%
ACTGCTTACAAACATGAGGG+TGG + chr5_3:56482412-56482431 Msa0819730:CDS 45.0%
TTACAAACATGAGGGTGGTG+TGG + chr5_3:56482417-56482436 Msa0819730:CDS 45.0%
GGTGGTGTGGTTATAAATCC+AGG + chr5_3:56482430-56482449 Msa0819730:CDS 45.0%
TCCCTGCTCTTTGAACACTT+CGG + chr5_3:56482611-56482630 Msa0819730:exon 45.0%
! ACCGAAGTGTTCAAAGAGCA+GGG - chr5_3:56482615-56482634 None:intergenic 45.0%
! GTACCCCTTTTACCAGTGAT+TGG + chr5_3:56482818-56482837 Msa0819730:three_prime_UTR 45.0%
AGAGTTGTCACTCGACCGAT+CGG - chr5_3:56479052-56479071 None:intergenic 50.0%
! CTTCTGATCGAAAGCGTGAC+CGG + chr5_3:56479508-56479527 Msa0819730:intron 50.0%
GGTGTCAATGGGTCAGTGTT+TGG + chr5_3:56479583-56479602 Msa0819730:intron 50.0%
ATGTGGTGTCTCGATCACGT+TGG + chr5_3:56479826-56479845 Msa0819730:intron 50.0%
TTGATGCTCCCGATGCTTCT+CGG + chr5_3:56479866-56479885 Msa0819730:intron 50.0%
GGGATATGCACATACCCCAT+AGG + chr5_3:56480672-56480691 Msa0819730:CDS 50.0%
AGGAATATCAGGCGCCCTAT+GGG - chr5_3:56480690-56480709 None:intergenic 50.0%
TGATGTGCTGGATCTTGCCA+GGG - chr5_3:56480736-56480755 None:intergenic 50.0%
ATGATGTGCTGGATCTTGCC+AGG - chr5_3:56480737-56480756 None:intergenic 50.0%
! CACAGTAGCACTGAAGGTAC+AGG - chr5_3:56481232-56481251 None:intergenic 50.0%
GTAGGTTGTGGGTTCCACTT+TGG + chr5_3:56481286-56481305 Msa0819730:intron 50.0%
GGGATTATGCCATGGTCATC+AGG + chr5_3:56481803-56481822 Msa0819730:CDS 50.0%
!! GTTGAGAGGGTGCTTCACTT+TGG + chr5_3:56481894-56481913 Msa0819730:intron 50.0%
GTAGCATCGCAAGTGAGTCT+AGG - chr5_3:56482342-56482361 None:intergenic 50.0%
GTCATAGGTCATGCTGCTGT+AGG - chr5_3:56482470-56482489 None:intergenic 50.0%
! GACCGAAGTGTTCAAAGAGC+AGG - chr5_3:56482616-56482635 None:intergenic 50.0%
TCTACCACCCAGCTTCCGAT+CGG + chr5_3:56479034-56479053 Msa0819730:five_prime_UTR 55.0%
CTCCGCTCCGCCATTAACAA+AGG + chr5_3:56479074-56479093 Msa0819730:five_prime_UTR 55.0%
TGATGCTCCCGATGCTTCTC+GGG + chr5_3:56479867-56479886 Msa0819730:intron 55.0%
! TGACGAGTCCCGAGAAGCAT+CGG - chr5_3:56479878-56479897 None:intergenic 55.0%
!! ATGGTGTTGGTCTTGGCCCT+GGG + chr5_3:56480650-56480669 Msa0819730:CDS 55.0%
GGGTATGTGCATATCCCCCA+GGG - chr5_3:56480669-56480688 None:intergenic 55.0%
GGAATATCAGGCGCCCTATG+GGG - chr5_3:56480689-56480708 None:intergenic 55.0%
CAGGAATATCAGGCGCCCTA+TGG - chr5_3:56480691-56480710 None:intergenic 55.0%
GATCTTGCCAGGGACAAGCA+TGG - chr5_3:56480726-56480745 None:intergenic 55.0%
GGTTGTGGGTTCCACTTTGG+TGG + chr5_3:56481289-56481308 Msa0819730:intron 55.0%
AGTGAGTCTAGGTCTCCCCA+TGG - chr5_3:56482331-56482350 None:intergenic 55.0%
GATGGGTGTGACCTGTGACA+AGG - chr5_3:56482387-56482406 None:intergenic 55.0%
TAGGCACCAGATGCAGAACC+TGG - chr5_3:56482451-56482470 None:intergenic 55.0%
CATTGATGCTCTGCGTGTCG+TGG + chr5_3:56482516-56482535 Msa0819730:CDS 55.0%
!! AATTGGTGCCGCTCTCCTCC+CGG + chr5_3:56478997-56479016 Msa0819730:five_prime_UTR 60.0%
ACCAAACACCGGGAGGAGAG+CGG - chr5_3:56479008-56479027 None:intergenic 60.0%
CACTCGACCGATCGGAAGCT+GGG - chr5_3:56479044-56479063 None:intergenic 60.0%
TCACTCGACCGATCGGAAGC+TGG - chr5_3:56479045-56479064 None:intergenic 60.0%
!! GACGAGTCCCGAGAAGCATC+GGG - chr5_3:56479877-56479896 None:intergenic 60.0%
!! GATGGTGTTGGTCTTGGCCC+TGG + chr5_3:56480649-56480668 Msa0819730:exon 60.0%
!! TGGTGTTGGTCTTGGCCCTG+GGG + chr5_3:56480651-56480670 Msa0819730:CDS 60.0%
GGGGTATGTGCATATCCCCC+AGG - chr5_3:56480670-56480689 None:intergenic 60.0%
TCGACCGATCGGAAGCTGGG+TGG - chr5_3:56479041-56479060 None:intergenic 65.0%
!! GGTGTTGGTCTTGGCCCTGG+GGG + chr5_3:56480652-56480671 Msa0819730:CDS 65.0%
GCCGCTCTCCTCCCGGTGTT+TGG + chr5_3:56479004-56479023 Msa0819730:five_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 56478947 56482909 56478947 ID=Msa0819730;Name=Msa0819730
chr5_3 mRNA 56478947 56482909 56478947 ID=Msa0819730-mRNA-1;Parent=Msa0819730;Name=Msa0819730-mRNA-1;_AED=0.02;_eAED=0.02;_QI=185|1|1|1|0.66|0.5|4|285|190
chr5_3 exon 56478947 56479095 56478947 ID=Msa0819730-mRNA-1:exon:17036;Parent=Msa0819730-mRNA-1
chr5_3 exon 56480614 56480781 56480614 ID=Msa0819730-mRNA-1:exon:17037;Parent=Msa0819730-mRNA-1
chr5_3 exon 56481687 56481824 56481687 ID=Msa0819730-mRNA-1:exon:17038;Parent=Msa0819730-mRNA-1
chr5_3 exon 56482322 56482909 56482322 ID=Msa0819730-mRNA-1:exon:17039;Parent=Msa0819730-mRNA-1
chr5_3 five_prime_UTR 56478947 56479095 56478947 ID=Msa0819730-mRNA-1:five_prime_utr;Parent=Msa0819730-mRNA-1
chr5_3 five_prime_UTR 56480614 56480649 56480614 ID=Msa0819730-mRNA-1:five_prime_utr;Parent=Msa0819730-mRNA-1
chr5_3 CDS 56480650 56480781 56480650 ID=Msa0819730-mRNA-1:cds;Parent=Msa0819730-mRNA-1
chr5_3 CDS 56481687 56481824 56481687 ID=Msa0819730-mRNA-1:cds;Parent=Msa0819730-mRNA-1
chr5_3 CDS 56482322 56482624 56482322 ID=Msa0819730-mRNA-1:cds;Parent=Msa0819730-mRNA-1
chr5_3 three_prime_UTR 56482625 56482909 56482625 ID=Msa0819730-mRNA-1:three_prime_utr;Parent=Msa0819730-mRNA-1
Gene Sequence

>Msa0819730

ATGGTGTTGGTCTTGGCCCTGGGGGATATGCACATACCCCATAGGGCGCCTGATATTCCTGCAAAGTTCAAATCCATGCTTGTCCCTGGCAAGATCCAGCACATCATTTGTACTGGAAACTTGTGTATTAAAGAAGTCCATGACTACCTAAAGACTCTTTGTCCCGATTTGCATATAACTCGTGGTGAGTATGATGAAGATTCAAGATATCCAGAGACCAAGACACTAACTATTGGCCAATTTAAGTTGGGATTATGCCATGGTCATCAGGTTATTCCATGGGGAGACCTAGACTCACTTGCGATGCTACAGAGACAGCTTGATGTAGACATCCTTGTCACAGGTCACACCCATCAGTTTACTGCTTACAAACATGAGGGTGGTGTGGTTATAAATCCAGGTTCTGCATCTGGTGCCTACAGCAGCATGACCTATGACGTGAACCCAAGTTTTGTGCTTATGGACATTGATGCTCTGCGTGTCGTGGTCTATGTATATGAACTTATTGATGGAGAGGTTAAGGTTGACAAGATTGATTTTAAGAAAACATCGACAAGCGTCCCTGCTCTTTGA
Protein sequence

>Msa0819730

MVLVLALGDMHIPHRAPDIPAKFKSMLVPGKIQHIICTGNLCIKEVHDYLKTLCPDLHITRGEYDEDSRYPETKTLTIGQFKLGLCHGHQVIPWGDLDSLAMLQRQLDVDILVTGHTHQFTAYKHEGGVVINPGSASGAYSSMTYDVNPSFVLMDIDALRVVVYVYELIDGEVKVDKIDFKKTSTSVPAL*