AlfalfaGEDB | Msa0973320

M. sativa cultivar zhongmu-4 / Msa0973320

KEGG: -
GO: GO:0002831 GO:0002832 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0008150 GO:0009507 GO:0009536 GO:0016020 GO:0031347 GO:0031348 GO:0032101 GO:0032102 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043900 GO:0043901 GO:0044424 GO:0044444 GO:0044464 GO:0048519 GO:0048583 GO:0048585 GO:0050789 GO:0065007 GO:0071944 GO:0080134 GO:1900150 GO:1900424 GO:1900425
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0973320	XP_003618423.1	96.674	481	7	1	1	472	122	602	0.0	951

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0973320	sp\|Q9LSI9\|BIR2_ARATH	63.299	485	156	6	1	470	126	603	0.0	612

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0973320	G7KK02	96.674	481	7	1	1	472	122	602	0.0	951

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0007850	Msa0973320	0.834510	2.875197e-56	-8.615850e-47
Msa0010490	Msa0973320	0.824440	8.062153e-54	-8.615850e-47
Msa0010500	Msa0973320	0.815182	1.053961e-51	-8.615850e-47
Msa0010770	Msa0973320	0.808006	3.828558e-50	-8.615850e-47
Msa0012710	Msa0973320	0.812202	4.774587e-51	-8.615850e-47
Msa0017320	Msa0973320	0.819941	8.916065e-53	-8.615850e-47
Msa0020390	Msa0973320	0.821404	4.112057e-53	-8.615850e-47
Msa0026150	Msa0973320	0.806411	8.332708e-50	-8.615850e-47
Msa0030280	Msa0973320	0.805017	1.635164e-49	-8.615850e-47
Msa0030300	Msa0973320	0.811923	5.491799e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0973320	MtrunA17_Chr6g0452221	96.674	481	7	1	1	472	122	602	0.0	951
Msa0973320	MtrunA17_Chr7g0249841	49.045	471	219	8	3	458	127	591	6.57e-146	430
Msa0973320	MtrunA17_Chr8g0337671	45.474	475	236	8	1	458	127	595	2.72e-133	398
Msa0973320	MtrunA17_Chr3g0129391	37.474	483	261	13	1	447	673	1150	8.81e-80	269
Msa0973320	MtrunA17_Chr1g0203631	36.534	479	268	12	1	450	800	1271	2.90e-76	259
Msa0973320	MtrunA17_Chr3g0079661	35.000	480	278	10	1	450	719	1194	5.97e-76	258
Msa0973320	MtrunA17_Chr8g0345561	35.876	485	263	13	4	450	612	1086	2.68e-69	239
Msa0973320	MtrunA17_Chr1g0169841	35.394	469	248	11	5	440	636	1082	3.95e-68	236
Msa0973320	MtrunA17_Chr2g0317071	34.959	492	261	12	1	450	597	1071	7.61e-68	234
Msa0973320	MtrunA17_Chr8g0382101	34.194	465	267	10	4	446	112	559	3.27e-66	223

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0973320	AT3G28450.1	63.299	485	156	6	1	470	126	603	0.0	612
Msa0973320	AT1G27190.1	61.856	485	164	7	1	468	120	600	0.0	568
Msa0973320	AT1G69990.1	56.907	485	185	9	1	469	113	589	0.0	540
Msa0973320	AT5G48380.1	44.421	484	236	10	1	458	124	600	5.92e-131	392
Msa0973320	AT1G55610.1	34.834	511	287	12	1	471	662	1166	6.29e-81	272
Msa0973320	AT1G55610.2	34.834	511	287	12	1	471	662	1166	6.29e-81	272
Msa0973320	AT5G07280.1	38.462	481	251	12	1	447	723	1192	2.53e-78	265
Msa0973320	AT3G13380.1	34.570	512	285	14	1	471	662	1164	1.18e-75	258
Msa0973320	AT4G39400.1	36.475	488	263	13	1	447	677	1158	3.26e-75	256
Msa0973320	AT5G53890.1	36.943	471	260	12	7	447	565	1028	9.26e-73	248

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Find 125 sgRNAs with CRISPR-Local

Find 308 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGGGATGTGTATGGATT+CGG	0.443517	6_4:-5784434	Msa0973320:CDS
AACATCGACGAAGAATTTAA+AGG	0.331687	6_4:-5784362	Msa0973320:CDS
AACTAAGCCGGCTCATTCTC+TGG	0.402380	6_4:+5784093	None:intergenic
AAGGAAGGGTATGTTGTCGG+CGG	0.652830	6_4:-5785106	Msa0973320:CDS
AAGTGGAAAAGGTAACGATG+AGG	0.682466	6_4:-5784271	Msa0973320:CDS
ACAACACAATACCCCAACAA+AGG	0.679027	6_4:+5784800	None:intergenic
ACAGGTTAGGCATCCGAATT+TGG	0.250636	6_4:-5784825	Msa0973320:CDS
ACCATCGTACTCGAATACTC+AGG	0.677492	6_4:+5784467	None:intergenic
ACCGCGAGCGTCGAACCATC+CGG	0.472091	6_4:+5784911	None:intergenic
ACGATGGTTGCTTCGTTGAA+AGG	0.518211	6_4:-5784452	Msa0973320:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGGTATGAGTAAGAAGAATT+TGG	-	chr6_4:5784376-5784395	Msa0973320:CDS	30.0%
AGAATGTTCTTATTACGACT+AGG	-	chr6_4:5784651-5784670	Msa0973320:CDS	30.0%
ATATTCTGTTGTATAATCGG+AGG	+	chr6_4:5784992-5785011	None:intergenic	30.0%
AACATCGACGAAGAATTTAA+AGG	-	chr6_4:5785250-5785269	Msa0973320:CDS	30.0%
CGAAGAATTTAAAGGCAATT+TGG	-	chr6_4:5785258-5785277	Msa0973320:CDS	30.0%
CCTTCAACGAATTATAAACT+TGG	+	chr6_4:5785437-5785456	None:intergenic	30.0%
CCAAGTTTATAATTCGTTGA+AGG	-	chr6_4:5785434-5785453	Msa0973320:CDS	30.0%
TGGTATGAGTAAGAAGAATT+TGG	-	chr6_4:5784376-5784395	Msa0973320:CDS	30.0%
AGAATGTTCTTATTACGACT+AGG	-	chr6_4:5784651-5784670	Msa0973320:CDS	30.0%
ATATTCTGTTGTATAATCGG+AGG	+	chr6_4:5784992-5785011	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_4	gene	5784095	5785539	5784095	ID=Msa0973320;Name=Msa0973320
chr6_4	mRNA	5784095	5785539	5784095	ID=Msa0973320-mRNA-1;Parent=Msa0973320;Name=Msa0973320-mRNA-1;_AED=0.03;_eAED=0.04;_QI=0\|0\|0\|1\|1\|1\|2\|0\|472
chr6_4	exon	5784347	5785539	5784347	ID=Msa0973320-mRNA-1:exon:2451;Parent=Msa0973320-mRNA-1
chr6_4	exon	5784095	5784320	5784095	ID=Msa0973320-mRNA-1:exon:2450;Parent=Msa0973320-mRNA-1
chr6_4	CDS	5784347	5785539	5784347	ID=Msa0973320-mRNA-1:cds;Parent=Msa0973320-mRNA-1
chr6_4	CDS	5784095	5784320	5784095	ID=Msa0973320-mRNA-1:cds;Parent=Msa0973320-mRNA-1

Gene Sequence

>Msa0973320

ATGCCTTTTTTGGTAACTTTGGATTTATCTGGTAATAATTTGAATGGTGAAATTCCACATACTCTTGTGAATTGTACTTATTTGAATGAGTTAATGTTGGATAATAATCATTTAAGTGGTAGTATACCTTATGAGATTACTAGTTTAACTAGGTTACATAAGTTTAGTGTTGCTAATAATCAATTAAGTGGTGATATACCATCTTTTTTTAATGGTTTTGATAAGGATGGTTTTGATGGGAATAGTGGTTTATGTGGTGGTCCACTTGGATCAAAGTGTGGTGGTATGAGTAAGAAGAATTTGGCTATTATTATTGCTGCTGGTGTTTTTGGTGCTGCTGGTTCTTTGTTGGCTGCTTTTGGATTGTGGTGGTGGTATCATTTGAGGTTAGGTGGGGAGAGGAGGAGGAGTAAGGAAGGGTATGTTGTCGGCGGGGTTGATGATTGGGCGGTTAGGTTGAGAGGTCATAAGCTTGCTCAAGTTAATCTTTTTCAGAAACCGATTGTTAAGGTGAAGCTTGGTGATTTGATGGCTGCTACGAATAATTTTAGTAATGAGAATGTTCTTATTACGACTAGGACGGGGGCGACTTATAGGGCGGATCTTCCGGATGGTTCGACGCTCGCGGTGAAGAGGTTGAGTAGTTGTAAGATTGGCGAGAAGCAGTTTCGGATGGAGATGAATCGGTTGGGACAGGTTAGGCATCCGAATTTGGCACCTTTGTTGGGGTATTGTGTTGTGGAAGAGGAGAAGCTTTTGGTTTATAAGCATATGTCAAACGGGACGCTTTATTCGTTGTTGCATAAGAATAGCGGTGTGTTGGATTGGTTGATGAGGTTTAGGATTGGGTTGGGAGCGGCTCGGGGACTTGCTTGGTTGCATCATGGGTGTCATCCTCCGATTATACAACAGAATATTTGTTCGAATGTGATTCTTGTTGATGAAGAATTCGATGCTCGGATAATGGACTTTGGACTTGCCAGGCTTATGACTTCTGACGCCAATGGTAGTTTTGTGAACGGAGATTTGGGGGAACTCGGTTATATTGCTCCTGAGTATTCGAGTACGATGGTTGCTTCGTTGAAAGGGGATGTGTATGGATTCGGAGTTTTACTTTTGGAGCTGGTCACCGGGTGCAAACCTCTCGAGGTGAATAACATCGACGAAGAATTTAAAGGCAATTTGGTGATTGGACTCAAGGACTGCATCGATAGATCGATAAGTGGAAAAGGTAACGATGAGGAGATTCTGCAGTTTCTGAAAATCGCATCCAATTGTGTCATTGCTCGCGCAAAAGATAGGTGGTCTATGTACCAAGTTTATAATTCGTTGAAGGGCATTTCCAAAGACCACAGTTTCTCCGAACACGATGACGAATTTCCCTTGATCTTTGGTAAGCCAGAGAATGAGCCGGCTTAG

Protein sequence

>Msa0973320

MPFLVTLDLSGNNLNGEIPHTLVNCTYLNELMLDNNHLSGSIPYEITSLTRLHKFSVANNQLSGDIPSFFNGFDKDGFDGNSGLCGGPLGSKCGGMSKKNLAIIIAAGVFGAAGSLLAAFGLWWWYHLRLGGERRRSKEGYVVGGVDDWAVRLRGHKLAQVNLFQKPIVKVKLGDLMAATNNFSNENVLITTRTGATYRADLPDGSTLAVKRLSSCKIGEKQFRMEMNRLGQVRHPNLAPLLGYCVVEEEKLLVYKHMSNGTLYSLLHKNSGVLDWLMRFRIGLGAARGLAWLHHGCHPPIIQQNICSNVILVDEEFDARIMDFGLARLMTSDANGSFVNGDLGELGYIAPEYSSTMVASLKGDVYGFGVLLLELVTGCKPLEVNNIDEEFKGNLVIGLKDCIDRSISGKGNDEEILQFLKIASNCVIARAKDRWSMYQVYNSLKGISKDHSFSEHDDEFPLIFGKPENEPA*