AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0859710

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0859710 MtrunA17_Chr5g0438731 98.624 436 5 1 1 435 32 467 0.0 879
Msa0859710 MtrunA17_Chr2g0287471 84.962 133 20 0 1 133 1 133 4.50e-73 233
Msa0859710 MtrunA17_Chr2g0287471 43.119 109 61 1 156 264 26 133 4.81e-23 99.8
Msa0859710 MtrunA17_Chr7g0271721 33.704 270 153 8 1 267 3 249 2.94e-39 144
Msa0859710 MtrunA17_Chr7g0271721 41.538 130 68 4 157 280 23 150 6.48e-21 93.6
Msa0859710 MtrunA17_Chr1g0194961 34.583 240 137 5 33 267 34 258 2.95e-39 144
Msa0859710 MtrunA17_Chr1g0194961 40.541 148 70 4 28 172 147 279 1.67e-24 103
Msa0859710 MtrunA17_Chr1g0194961 39.844 128 69 4 163 284 34 159 5.76e-19 88.2
Msa0859710 MtrunA17_Chr1g0200731 40.351 114 59 4 30 136 95 206 9.02e-17 82.4
Msa0859710 MtrunA17_Chr1g0200731 29.148 223 134 5 163 382 98 299 2.25e-15 78.2
Msa0859710 MtrunA17_Chr3g0124851 41.284 109 57 4 30 132 35 142 1.29e-16 82.0
Msa0859710 MtrunA17_Chr3g0124851 34.302 172 94 7 163 329 38 195 3.32e-16 80.9
Msa0859710 MtrunA17_Chr3g0124851 33.333 105 68 1 26 128 375 479 7.96e-12 67.0
Msa0859710 MtrunA17_Chr8g0360031 41.463 82 48 0 30 111 73 154 2.22e-14 71.2
Msa0859710 MtrunA17_Chr8g0360031 25.131 191 123 6 72 259 2 175 1.87e-12 65.5
Msa0859710 MtrunA17_Chr2g0326581 40.800 125 66 4 135 256 55 174 4.03e-14 74.3
Msa0859710 MtrunA17_Chr2g0326581 51.220 82 38 2 30 110 80 160 2.92e-13 71.6
Msa0859710 MtrunA17_Chr2g0326581 32.609 138 87 3 36 169 424 559 1.30e-11 66.6
Msa0859710 MtrunA17_Chr2g0326581 26.699 206 133 6 64 259 322 519 3.53e-11 65.1
Msa0859710 MtrunA17_Chr2g0326241 28.814 118 81 2 17 132 29 145 9.35e-14 68.9
Msa0859710 MtrunA17_Chr2g0326241 28.333 120 83 2 156 273 37 155 7.37e-12 63.5
Msa0859710 MtrunA17_Chr1g0211871 34.483 116 72 2 24 138 64 176 1.13e-13 69.3
Msa0859710 MtrunA17_Chr1g0211871 29.730 111 74 1 153 263 63 169 1.39e-12 65.9
Msa0859710 MtrunA17_Chr2g0317731 26.174 149 100 2 107 245 9 157 1.63e-13 68.6
Msa0859710 MtrunA17_Chr2g0317731 34.653 101 66 0 11 111 51 151 2.56e-13 67.8
Msa0859710 MtrunA17_Chr1g0201781 32.558 86 58 0 34 119 66 151 5.24e-13 67.0
Msa0859710 MtrunA17_Chr1g0201781 30.769 104 65 3 166 268 68 165 5.31e-12 63.9
Msa0859710 MtrunA17_Chr4g0029181 48.421 95 40 5 30 120 102 191 1.16e-12 69.7
Msa0859710 MtrunA17_Chr2g0317721 34.513 113 68 1 5 111 10 122 4.14e-12 63.5
Msa0859710 MtrunA17_Chr3g0125821 35.632 87 56 0 33 119 73 159 7.45e-12 63.9
Msa0859710 MtrunA17_Chr4g0041801 32.967 91 52 2 160 242 143 232 1.08e-11 66.6
Msa0859710 MtrunA17_Chr2g0317711 33.628 113 69 1 5 111 10 122 1.29e-11 62.4
Msa0859710 MtrunA17_Chr4g0044731 36.905 84 50 1 31 111 63 146 4.51e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0859710 AT1G04980.1 72.523 444 112 4 1 435 5 447 0.0 678
Msa0859710 AT2G32920.1 73.474 426 108 3 14 435 16 440 0.0 654
Msa0859710 AT2G47470.2 35.833 240 134 5 33 267 28 252 1.65e-43 154
Msa0859710 AT2G47470.2 54.369 103 42 3 30 128 143 244 1.59e-27 110
Msa0859710 AT2G47470.2 38.583 127 72 3 163 284 28 153 3.64e-19 87.4
Msa0859710 AT2G47470.4 35.833 240 134 5 33 267 28 252 3.63e-43 155
Msa0859710 AT2G47470.4 54.369 103 42 3 30 128 143 244 1.76e-27 112
Msa0859710 AT2G47470.4 38.583 127 72 3 163 284 28 153 7.75e-19 87.8
Msa0859710 AT2G47470.3 35.833 240 134 5 33 267 28 252 3.69e-43 154
Msa0859710 AT2G47470.3 54.369 103 42 3 30 128 143 244 1.64e-27 112
Msa0859710 AT2G47470.3 38.583 127 72 3 163 284 28 153 7.89e-19 87.4
Msa0859710 AT2G47470.1 35.833 240 134 5 33 267 28 252 4.99e-43 155
Msa0859710 AT2G47470.1 54.369 103 42 3 30 128 143 244 1.88e-27 112
Msa0859710 AT2G47470.1 38.583 127 72 3 163 284 28 153 8.73e-19 87.8
Msa0859710 AT3G54960.2 46.154 91 48 1 30 120 101 190 1.09e-18 88.6
Msa0859710 AT3G54960.2 27.727 220 137 6 163 380 104 303 6.88e-15 77.0
Msa0859710 AT3G54960.1 46.154 91 48 1 30 120 101 190 1.45e-18 88.6
Msa0859710 AT3G54960.1 27.727 220 137 6 163 380 104 303 9.02e-15 77.0
Msa0859710 AT3G54960.1 37.363 91 49 2 36 126 445 527 3.59e-11 65.5
Msa0859710 AT5G60640.1 36.493 211 111 10 30 226 105 306 1.54e-17 85.5
Msa0859710 AT5G60640.1 41.414 99 50 4 161 256 106 199 1.28e-14 76.3
Msa0859710 AT5G60640.1 26.238 202 131 5 68 259 351 544 1.73e-11 66.6
Msa0859710 AT5G60640.2 36.493 211 111 10 30 226 105 306 1.85e-17 85.1
Msa0859710 AT5G60640.2 41.414 99 50 4 161 256 106 199 1.67e-14 75.9
Msa0859710 AT5G60640.2 26.131 199 130 5 62 252 346 535 7.83e-11 64.3
Msa0859710 AT5G60640.3 36.493 211 111 10 30 226 105 306 1.85e-17 85.1
Msa0859710 AT5G60640.3 41.414 99 50 4 161 256 106 199 9.87e-15 76.6
Msa0859710 AT1G21750.2 40.367 109 58 4 30 132 32 139 3.87e-17 84.0
Msa0859710 AT1G21750.2 34.906 106 63 3 163 263 35 139 2.63e-14 75.1
Msa0859710 AT1G21750.2 37.113 97 59 1 26 120 372 468 1.14e-12 70.1
Msa0859710 AT1G21750.1 40.367 109 58 4 30 132 32 139 4.04e-17 84.0
Msa0859710 AT1G21750.1 28.916 166 100 5 163 323 35 187 3.00e-14 75.1
Msa0859710 AT1G21750.1 37.113 97 59 1 26 120 372 468 1.03e-12 70.1
Msa0859710 AT1G21750.1 34.615 104 65 2 165 266 381 483 9.16e-11 64.3
Msa0859710 AT1G77510.1 39.450 109 59 4 30 132 31 138 3.02e-16 81.3
Msa0859710 AT1G77510.1 27.895 190 118 6 152 336 23 198 3.68e-16 80.9
Msa0859710 AT1G77510.1 29.927 137 92 2 26 160 370 504 4.53e-12 68.2
Msa0859710 AT1G07960.4 30.000 120 81 2 28 144 25 144 1.61e-14 71.2
Msa0859710 AT1G07960.4 25.439 114 82 2 154 265 21 133 1.16e-13 68.6
Msa0859710 AT1G07960.3 30.000 120 81 2 28 144 25 144 1.61e-14 71.2
Msa0859710 AT1G07960.3 25.439 114 82 2 154 265 21 133 1.16e-13 68.6
Msa0859710 AT1G07960.1 30.000 120 81 2 28 144 25 144 1.61e-14 71.2
Msa0859710 AT1G07960.1 25.439 114 82 2 154 265 21 133 1.16e-13 68.6
Msa0859710 AT1G07960.2 30.000 120 81 2 28 144 25 144 1.61e-14 71.2
Msa0859710 AT1G07960.2 25.439 114 82 2 154 265 21 133 1.16e-13 68.6
Msa0859710 AT1G35620.1 40.426 94 51 3 30 120 34 125 2.30e-14 75.1
Msa0859710 AT1G35620.1 28.390 236 137 10 155 383 29 239 2.45e-14 75.1
Msa0859710 AT1G43560.1 37.209 86 54 0 34 119 64 149 1.62e-13 68.9
Msa0859710 AT1G76760.1 31.429 105 68 1 34 138 69 169 2.02e-13 68.6
Msa0859710 AT4G03520.1 37.113 97 58 2 151 245 74 169 9.29e-13 67.0
Msa0859710 AT3G15360.1 36.667 90 57 0 30 119 88 177 4.97e-12 65.1
Msa0859710 AT1G03680.1 27.564 156 93 4 110 245 8 163 7.57e-12 64.3
Msa0859710 AT1G50320.1 29.333 150 90 4 121 263 30 170 1.05e-11 63.9
Msa0859710 AT4G27080.1 32.000 125 67 4 126 242 118 232 1.54e-11 66.6
Msa0859710 AT4G27080.2 32.000 125 67 4 126 242 170 284 1.89e-11 66.2
Msa0859710 AT3G20560.1 34.000 100 57 2 151 242 134 232 5.79e-11 64.7

Find 147 sgRNAs with CRISPR-Local

Find 309 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCTTCCCTTTCAAATTAT+TGG 0.104762 5_4:-58165783 None:intergenic
TTCAATCAATTGTTCATTAA+TGG 0.149709 5_4:-58167645 None:intergenic
TTCCCAACAATCTTGGTATT+TGG 0.173156 5_4:+58166203 Msa0859710:CDS
TTCCTTCCTTCCCGACATTT+TGG 0.184244 5_4:+58166483 Msa0859710:CDS
CTTAATTCCCTTCAGGAATA+TGG 0.210374 5_4:+58164998 Msa0859710:intron
TACGAGAAGACCTGGTTCTT+TGG 0.276020 5_4:+58167674 Msa0859710:three_prime_UTR
GAAGGCAGCTACTGTATTAA+AGG 0.276220 5_4:+58164040 Msa0859710:CDS
GATAGTATGTAGAATATTAA+AGG 0.283108 5_4:+58167452 Msa0859710:three_prime_UTR
CTCTAGTAACTTCGATGAAT+TGG 0.285509 5_4:+58165555 Msa0859710:CDS
CTTCAGGAATATGGTATTAG+AGG 0.289766 5_4:+58165007 Msa0859710:intron
CTATATAGCTCTGTCACTTC+TGG 0.297987 5_4:-58166323 None:intergenic
CGCAGAATTTGCACTTCAAC+AGG 0.299216 5_4:+58165105 Msa0859710:CDS
TCAATCAATTGTTCATTAAT+GGG 0.300305 5_4:-58167644 None:intergenic
GTTTCCTTATAGTTCATCTT+TGG 0.305542 5_4:-58167368 None:intergenic
TTTGAAAGGGAAGGTTAAAC+TGG 0.309941 5_4:+58165790 Msa0859710:CDS
GTAAGGCTCTGACTCCTATA+TGG 0.316417 5_4:+58164015 Msa0859710:CDS
TGCGATTGGTTTAACATCTC+TGG 0.351595 5_4:-58165086 None:intergenic
AATTTATATAGACTCGTAAA+TGG 0.356732 5_4:-58167971 None:intergenic
CTCAAGGTCTGGCTGCTTAC+CGG 0.356762 5_4:-58166988 None:intergenic
GCAAAAGGGGCTTAGATATA+GGG 0.370495 5_4:-58168005 None:intergenic
TAGAGGATTTCCAACTATAA+AGG 0.370686 5_4:+58165024 Msa0859710:CDS
GTGGCTTGAAACTCGTATTA+AGG 0.371667 5_4:+58167788 Msa0859710:three_prime_UTR
AACACCTTTCTTAACGTTCA+AGG 0.373568 5_4:-58167054 None:intergenic
TGCAAAAGGGGCTTAGATAT+AGG 0.373607 5_4:-58168006 None:intergenic
GCTCGAACGTTGCATCATAA+AGG 0.379435 5_4:-58167846 None:intergenic
CTCGAACGTTGCATCATAAA+GGG 0.386422 5_4:-58167845 None:intergenic
TCAACCAAAGATGAACTATA+AGG 0.387123 5_4:+58167364 Msa0859710:CDS
AAAGTTGGTGTTGGTGGGTA+TGG 0.390826 5_4:+58167014 Msa0859710:CDS
CAACGTTCGAGCTATGTTTA+CGG 0.396118 5_4:+58167857 Msa0859710:three_prime_UTR
GGGTCTTGAACAGCTATGTA+TGG 0.397598 5_4:+58166958 Msa0859710:intron
CTAGCACCCTCGTAAGGAAT+TGG 0.409040 5_4:-58166242 None:intergenic
CATCTCCTATAGCATAAAAC+TGG 0.409206 5_4:-58167430 None:intergenic
TCAAGGATTCCCAACAATCT+TGG 0.416145 5_4:+58166196 Msa0859710:CDS
TCCATGGTGCGGGCACTGTA+AGG 0.416505 5_4:+58163998 Msa0859710:CDS
ACCTTGGACTTGAAGTTGTT+AGG 0.419712 5_4:-58162562 None:intergenic
TTGTTGAATTCTTTGCTCCA+TGG 0.424265 5_4:+58163982 Msa0859710:CDS
TGCTGATGCTCACCAATCTC+TGG 0.427343 5_4:+58164088 Msa0859710:CDS
TTAGGAGTGAGTTGAAGAAC+GGG 0.430086 5_4:-58162544 None:intergenic
AGTTCAAAAGGAGTCCTTAC+AGG 0.430959 5_4:+58166563 Msa0859710:CDS
AGGAATTGGAGTATCTTTAT+CGG 0.435399 5_4:-58166228 None:intergenic
AGAAACCACCGCTTCTTCTT+CGG 0.438238 5_4:+58165522 Msa0859710:CDS
TCTGTTTCTCCCCTCCGCCT+TGG 0.439079 5_4:-58166509 None:intergenic
AGAGAATTTGTGAAAGAAGC+TGG 0.439854 5_4:+58167205 Msa0859710:intron
GTGTTGTCTATAGGATGTTT+TGG 0.440808 5_4:+58166426 Msa0859710:intron
TTATCAAAAGCAAAGAACTT+TGG 0.443575 5_4:+58165578 Msa0859710:CDS
GGTGCAAACACCTTTATAGT+TGG 0.448420 5_4:-58165034 None:intergenic
CCAACTTTCTGCTCAAGGTC+TGG 0.450805 5_4:-58166999 None:intergenic
TGCTCACCAATCTCTGGCTC+AGG 0.454025 5_4:+58164094 Msa0859710:CDS
GTTTAACATCTCTGGCTCCT+TGG 0.454708 5_4:-58165078 None:intergenic
TGTTGAATGTTGCACACAAC+CGG 0.455377 5_4:-58167718 None:intergenic
ATGAGCAGGTTCAATGTTCA+AGG 0.456443 5_4:+58166179 Msa0859710:CDS
AATTCTTTGCTCCATGGTGC+GGG 0.458259 5_4:+58163988 Msa0859710:CDS
ATCATTCGCACTAGAGCAGC+TGG 0.462465 5_4:+58166283 Msa0859710:CDS
TTATAGTTCATCTTTGGTTG+AGG 0.465932 5_4:-58167362 None:intergenic
TGACAGGTTCTGAATTCAAA+TGG 0.467668 5_4:+58163951 Msa0859710:intron
CTCCAAATACCAAGATTGTT+GGG 0.472339 5_4:-58166205 None:intergenic
ATCCTTTCTCATGCTCTCTA+CGG 0.472571 5_4:+58162511 Msa0859710:CDS
TTTGTCAGTCTCTAATGAGC+AGG 0.472766 5_4:+58166165 Msa0859710:intron
GGAAAACCTGAGCCAGAGAT+TGG 0.473756 5_4:-58164100 None:intergenic
CGGTTAAATGGAAAAGCAAC+AGG 0.474554 5_4:+58165481 Msa0859710:CDS
ATAGTATGTAGAATATTAAA+GGG 0.475068 5_4:+58167453 Msa0859710:three_prime_UTR
GCTCCAAATACCAAGATTGT+TGG 0.479181 5_4:-58166206 None:intergenic
ACGTTCAAGGCTACTAATGC+TGG 0.481617 5_4:-58167041 None:intergenic
GTTAGGAGTGAGTTGAAGAA+CGG 0.482632 5_4:-58162545 None:intergenic
AGTGAGTTGAAGAACGGGAG+AGG 0.483997 5_4:-58162539 None:intergenic
CAGTTGCAGAGAAGTTCAAA+AGG 0.484219 5_4:+58166551 Msa0859710:CDS
CTCGTATTAAGGTGAATAAG+TGG 0.484744 5_4:+58167799 Msa0859710:three_prime_UTR
GGTTCTGTCTTTACAATGGT+GGG 0.490337 5_4:-58167268 None:intergenic
ACTATAAAGGTGTTTGCACC+TGG 0.493971 5_4:+58165037 Msa0859710:CDS
GCCTTGGAATCCAAAATGTC+GGG 0.495320 5_4:-58166493 None:intergenic
GAACCATGGGATGGAAAAGA+TGG 0.495841 5_4:+58167286 Msa0859710:CDS
TGGTTCTGTCTTTACAATGG+TGG 0.496152 5_4:-58167269 None:intergenic
CGTTCCTCATTGACCATAGC+CGG 0.496245 5_4:+58167699 Msa0859710:three_prime_UTR
TTAGTGAAGTGTTTAGAAGA+TGG 0.498830 5_4:+58167936 Msa0859710:three_prime_UTR
AGAGCATCCAATAATTTGAA+AGG 0.498898 5_4:+58165776 Msa0859710:CDS
CGCCTTGGAATCCAAAATGT+CGG 0.513014 5_4:-58166494 None:intergenic
AACCGTAGAGAGCATGAGAA+AGG 0.514080 5_4:-58162513 None:intergenic
GAAGCTGGACGTGGAGGCAA+AGG 0.515570 5_4:+58167220 Msa0859710:CDS
AAGGCAGCTACTGTATTAAA+GGG 0.517489 5_4:+58164041 Msa0859710:CDS
GAGCATCCAATAATTTGAAA+GGG 0.518744 5_4:+58165777 Msa0859710:CDS
GTAGCTGCCTTCTCCCATAT+AGG 0.519121 5_4:-58164029 None:intergenic
ATGTTGCACACAACCGGCTA+TGG 0.522020 5_4:-58167712 None:intergenic
TGGTGGGAGGAGTGTCACCT+AGG 0.522614 5_4:-58167252 None:intergenic
ATCCAATAATTTGAAAGGGA+AGG 0.529134 5_4:+58165781 Msa0859710:CDS
TAAGGCTCTGACTCCTATAT+GGG 0.533987 5_4:+58164016 Msa0859710:CDS
CCAGACCTTGAGCAGAAAGT+TGG 0.535182 5_4:+58166999 Msa0859710:CDS
GGTGGGAGGAGTGTCACCTA+GGG 0.535529 5_4:-58167251 None:intergenic
CTGGCTCCTTGGTAATCGAC+TGG 0.539378 5_4:-58165067 None:intergenic
GAATTCTTTGCTCCATGGTG+CGG 0.548779 5_4:+58163987 Msa0859710:CDS
GAGCAGAAAGTTGGTGTTGG+TGG 0.549595 5_4:+58167008 Msa0859710:CDS
TCCTAACAACTTCAAGTCCA+AGG 0.552476 5_4:+58162561 Msa0859710:CDS
ATCCAAAATGTCGGGAAGGA+AGG 0.552840 5_4:-58166485 None:intergenic
CCATGGTTCTGTCTTTACAA+TGG 0.553296 5_4:-58167272 None:intergenic
GTAGCCTTGAACGTTAAGAA+AGG 0.556407 5_4:+58167050 Msa0859710:CDS
AACATACCTCAGGAAAATGT+TGG 0.556667 5_4:+58167600 Msa0859710:three_prime_UTR
AATCTACGCTTCTTCAACAA+TGG 0.558002 5_4:+58162441 Msa0859710:exon
CTCTAGTGCGAATGATTCGA+TGG 0.558140 5_4:-58166276 None:intergenic
GGTCTTGAACAGCTATGTAT+GGG 0.559621 5_4:+58166959 Msa0859710:intron
AAAAGATGGAGAAATAATTG+AGG 0.562273 5_4:+58167300 Msa0859710:CDS
TGAAGTGCAAATTCTGCGAT+TGG 0.562719 5_4:-58165100 None:intergenic
GCCTTACAGTGCCCGCACCA+TGG 0.567839 5_4:-58163999 None:intergenic
AACATTATTACGAGAAGACC+TGG 0.569546 5_4:+58167666 Msa0859710:three_prime_UTR
GTTCTTGTATGAAGAGAAGA+AGG 0.570808 5_4:-58162387 None:intergenic
CAACTGTTAATAACTGCTGA+AGG 0.571823 5_4:-58166534 None:intergenic
GCAGTTCTAGCACCCTCGTA+AGG 0.576634 5_4:-58166248 None:intergenic
CAACACCAACTTTCTGCTCA+AGG 0.577087 5_4:-58167004 None:intergenic
AGCAGAAAGTTGGTGTTGGT+GGG 0.581032 5_4:+58167009 Msa0859710:CDS
AAGCTGGACGTGGAGGCAAA+GGG 0.583780 5_4:+58167221 Msa0859710:CDS
TTTGTGAAAGAAGCTGGACG+TGG 0.587104 5_4:+58167211 Msa0859710:CDS
AGCTATGTATGGGCAGCTGC+CGG 0.590014 5_4:+58166969 Msa0859710:intron
TTCATGTCATTCTCATTCAG+TGG 0.591892 5_4:+58167574 Msa0859710:three_prime_UTR
GGCTTAGATATAGGGACGGA+GGG 0.591898 5_4:-58167997 None:intergenic
AGATTGATGAATGAATCTGC+GGG 0.594548 5_4:-58162423 None:intergenic
TCTGACTCCTATATGGGAGA+AGG 0.598675 5_4:+58164022 Msa0859710:CDS
TCTGTCTTTACAATGGTGGG+AGG 0.605063 5_4:-58167265 None:intergenic
TCGTATTAAGGTGAATAAGT+GGG 0.608145 5_4:+58167800 Msa0859710:three_prime_UTR
GCTCCTTGGTAATCGACTGG+TGG 0.611053 5_4:-58165064 None:intergenic
TAGTTCTACCGAAGAAGAAG+CGG 0.621191 5_4:-58165530 None:intergenic
AGTTCAAAAGCACTCTTGAG+AGG 0.621890 5_4:-58167083 None:intergenic
TCAGTGGTACAACATACCTC+AGG 0.622601 5_4:+58167590 Msa0859710:three_prime_UTR
CATTGTAAAGACAGAACCAT+GGG 0.623172 5_4:+58167273 Msa0859710:CDS
TTCTACCGAAGAAGAAGCGG+TGG 0.624355 5_4:-58165527 None:intergenic
GGCAAAGGGAACTTGCCCCT+AGG 0.627464 5_4:+58167235 Msa0859710:CDS
CTTGAGCAGAAAGTTGGTGT+TGG 0.628583 5_4:+58167005 Msa0859710:CDS
GAGGAACGATCCAAAGAACC+AGG 0.629610 5_4:-58167684 None:intergenic
TTGAAAGGGAAGGTTAAACT+GGG 0.631529 5_4:+58165791 Msa0859710:CDS
AACACTTCACTAATGCATGT+AGG 0.634863 5_4:-58167926 None:intergenic
TGGAATCCAAAATGTCGGGA+AGG 0.635224 5_4:-58166489 None:intergenic
GATACTCCAATTCCTTACGA+GGG 0.636183 5_4:+58166236 Msa0859710:CDS
TGTTGACTGTGATGCTGACA+AGG 0.636371 5_4:+58165817 Msa0859710:CDS
GTAAAGACAGAACCATGGGA+TGG 0.640244 5_4:+58167277 Msa0859710:CDS
TATAGCTCTGTCACTTCTGG+AGG 0.640322 5_4:-58166320 None:intergenic
TAGATTGATGAATGAATCTG+CGG 0.646073 5_4:-58162424 None:intergenic
GTGAAAGAAGCTGGACGTGG+AGG 0.650513 5_4:+58167214 Msa0859710:CDS
GGGCTTAGATATAGGGACGG+AGG 0.661411 5_4:-58167998 None:intergenic
TTAAATGGAAAAGCAACAGG+TGG 0.661631 5_4:+58165484 Msa0859710:CDS
AGATACTCCAATTCCTTACG+AGG 0.665654 5_4:+58166235 Msa0859710:CDS
TCGAACGTTGCATCATAAAG+GGG 0.668066 5_4:-58167844 None:intergenic
AAGACGAGTATAGTGATGAG+TGG 0.670606 5_4:+58167538 Msa0859710:three_prime_UTR
GTGGGAGGAGTGTCACCTAG+GGG 0.673404 5_4:-58167250 None:intergenic
GAATATTAAAGGGAAATGTG+TGG 0.677310 5_4:+58167463 Msa0859710:three_prime_UTR
ACAACCGGCTATGGTCAATG+AGG 0.692842 5_4:-58167703 None:intergenic
AAGGGGCTTAGATATAGGGA+CGG 0.695087 5_4:-58168001 None:intergenic
GATTGATGAATGAATCTGCG+GGG 0.699974 5_4:-58162422 None:intergenic
CCATTGTAAAGACAGAACCA+TGG 0.708421 5_4:+58167272 Msa0859710:CDS
ATTGATGAATGAATCTGCGG+GGG 0.723990 5_4:-58162421 None:intergenic
AAACCACCAGTCGATTACCA+AGG 0.727693 5_4:+58165061 Msa0859710:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCATTTTCTTAAATTATTAT+CGG + chr5_4:58163062-58163081 Msa0859710:intron 10.0%
!!! AAAATTATTCATATTTTCTT+GGG - chr5_4:58163860-58163879 None:intergenic 10.0%
!!! AAAAATTATTCATATTTTCT+TGG - chr5_4:58163861-58163880 None:intergenic 10.0%
!! AATTTAATGACATATATTAA+AGG + chr5_4:58164160-58164179 Msa0859710:intron 10.0%
!! GTAATAAATAATTGAAATAT+AGG - chr5_4:58165637-58165656 None:intergenic 10.0%
!! TATAAATAAATTGAAAAGTA+AGG - chr5_4:58166074-58166093 None:intergenic 10.0%
!! AATAATATTAAAAGACTGTT+TGG - chr5_4:58163806-58163825 None:intergenic 15.0%
!!! AAATTATTCATATTTTCTTG+GGG - chr5_4:58163859-58163878 None:intergenic 15.0%
!!! AATTTTAAATATTGAATCTG+AGG - chr5_4:58164144-58164163 None:intergenic 15.0%
!! TTATAGCTTATTTAAAAAAC+AGG + chr5_4:58164470-58164489 Msa0859710:intron 15.0%
!!! ATATTTAAAATAGTTACAAG+AGG - chr5_4:58164594-58164613 None:intergenic 15.0%
!!! TTGTAACTATTTTAAATATG+TGG + chr5_4:58164595-58164614 Msa0859710:intron 15.0%
!!! TAAAATTGTTTGATGTTTTA+AGG - chr5_4:58164660-58164679 None:intergenic 15.0%
!! AATATTTGATTGAAACTTTA+GGG - chr5_4:58165169-58165188 None:intergenic 15.0%
!! AAATATTTGATTGAAACTTT+AGG - chr5_4:58165170-58165189 None:intergenic 15.0%
!! TGAAAAATTACTACAAATAA+AGG + chr5_4:58165275-58165294 Msa0859710:intron 15.0%
!! AATTTGATTAATAACTTGAA+AGG + chr5_4:58165300-58165319 Msa0859710:intron 15.0%
!! ATTTGATTAATAACTTGAAA+GGG + chr5_4:58165301-58165320 Msa0859710:intron 15.0%
!! AAAAATAGACTTACATAATT+TGG - chr5_4:58167112-58167131 None:intergenic 15.0%
!! ATAGTATGTAGAATATTAAA+GGG + chr5_4:58167453-58167472 Msa0859710:three_prime_UTR 15.0%
!! TTGAAAAAAAGATTAAACCT+TGG - chr5_4:58162581-58162600 None:intergenic 20.0%
!! AAAAAACAAGCTAAAAAAGT+GGG - chr5_4:58162628-58162647 None:intergenic 20.0%
!!! TTTTAGCTTGTTTTTTCAAA+TGG + chr5_4:58162632-58162651 Msa0859710:intron 20.0%
!!! TTTAGCTTGTTTTTTCAAAT+GGG + chr5_4:58162633-58162652 Msa0859710:intron 20.0%
!! TATCATCTGAAAAAATCAAT+AGG + chr5_4:58162885-58162904 Msa0859710:intron 20.0%
!! TATCACTAAAATTTAGTTGA+AGG + chr5_4:58163204-58163223 Msa0859710:intron 20.0%
!!! AAGTGATTTTATGTTATGAT+AGG + chr5_4:58163229-58163248 Msa0859710:intron 20.0%
!!! CTTCAAGTAGAATATATTTT+GGG + chr5_4:58163326-58163345 Msa0859710:intron 20.0%
!!! TTCAAGTAGAATATATTTTG+GGG + chr5_4:58163327-58163346 Msa0859710:intron 20.0%
!!! AGAACTAATTTTTACATGTT+TGG - chr5_4:58163379-58163398 None:intergenic 20.0%
!! TACTAACTAAACTCAAATTA+AGG + chr5_4:58163545-58163564 Msa0859710:intron 20.0%
!! ACACATATTCATATTCATAA+AGG - chr5_4:58164225-58164244 None:intergenic 20.0%
!!! ATTAAGTTGTTTATCCAAAT+AGG + chr5_4:58164559-58164578 Msa0859710:intron 20.0%
!!! TTAAGTTGTTTATCCAAATA+GGG + chr5_4:58164560-58164579 Msa0859710:intron 20.0%
!! CGTTAATGTAATATATTCTT+TGG - chr5_4:58164773-58164792 None:intergenic 20.0%
!!! TGTCTTTTTTTATTTCTCAA+AGG + chr5_4:58165717-58165736 Msa0859710:intron 20.0%
!! TTTAACAAACTGTTATTGTT+TGG + chr5_4:58165926-58165945 Msa0859710:intron 20.0%
!! TCAGAATAATATAAACTGAA+AGG - chr5_4:58166130-58166149 None:intergenic 20.0%
!!! TCTTTCAAATTCAACTTTTT+CGG + chr5_4:58166656-58166675 Msa0859710:intron 20.0%
!! CATGTTTAAATCAAGATAAA+AGG + chr5_4:58166712-58166731 Msa0859710:intron 20.0%
!!! TTGCAGTTATTTTTAAATTG+CGG + chr5_4:58166794-58166813 Msa0859710:intron 20.0%
!! TACTTACATGGAAATATAAT+GGG + chr5_4:58166827-58166846 Msa0859710:intron 20.0%
!!! AATGGTTTTTTGTTTGAATT+TGG + chr5_4:58166937-58166956 Msa0859710:intron 20.0%
!!! ATGGTTTTTTGTTTGAATTT+GGG + chr5_4:58166938-58166957 Msa0859710:intron 20.0%
!!! CTAATTTTATGCTAAATAGT+TGG + chr5_4:58167132-58167151 Msa0859710:intron 20.0%
!! GATAGTATGTAGAATATTAA+AGG + chr5_4:58167452-58167471 Msa0859710:three_prime_UTR 20.0%
!!! TTTTTCAGATTTTTCTTGTT+CGG + chr5_4:58167510-58167529 Msa0859710:three_prime_UTR 20.0%
!! TCAATCAATTGTTCATTAAT+GGG - chr5_4:58167647-58167666 None:intergenic 20.0%
!! TTCAATCAATTGTTCATTAA+TGG - chr5_4:58167648-58167667 None:intergenic 20.0%
!!! TTTTTTTTAAAGTAGCATAG+TGG + chr5_4:58167769-58167788 Msa0859710:three_prime_UTR 20.0%
!! AATTTATATAGACTCGTAAA+TGG - chr5_4:58167974-58167993 None:intergenic 20.0%
! GAAAAAACAAGCTAAAAAAG+TGG - chr5_4:58162629-58162648 None:intergenic 25.0%
!! TTTTGTATCTGTTTTGTGTT+TGG + chr5_4:58162676-58162695 Msa0859710:intron 25.0%
!!! TATGGTTGATTTTATGATTC+TGG + chr5_4:58162762-58162781 Msa0859710:intron 25.0%
!!! ATGGTTGATTTTATGATTCT+GGG + chr5_4:58162763-58162782 Msa0859710:intron 25.0%
! AAATATATTCTACTTGAAGC+TGG - chr5_4:58163325-58163344 None:intergenic 25.0%
!! GCTTCAAGTAGAATATATTT+TGG + chr5_4:58163325-58163344 Msa0859710:intron 25.0%
!!! TCAAGTAGAATATATTTTGG+GGG + chr5_4:58163328-58163347 Msa0859710:intron 25.0%
!!! TATGTTTGATTTCACTTTTG+AGG - chr5_4:58163426-58163445 None:intergenic 25.0%
!! TATCTAGTTTGTAATACATC+AGG + chr5_4:58163904-58163923 Msa0859710:intron 25.0%
!! TGTAATACATCAGGTTTTTA+TGG + chr5_4:58163913-58163932 Msa0859710:intron 25.0%
!! GTAATACATCAGGTTTTTAT+GGG + chr5_4:58163914-58163933 Msa0859710:intron 25.0%
! TAATTAAGCACTTATCATGT+AGG - chr5_4:58164543-58164562 None:intergenic 25.0%
!! TAGTGTTACAATTTGGATTT+TGG + chr5_4:58165200-58165219 Msa0859710:intron 25.0%
! AGTGAGAATATCAATGTATT+GGG + chr5_4:58165370-58165389 Msa0859710:intron 25.0%
! AATATCAATGTATTGGGATT+AGG + chr5_4:58165376-58165395 Msa0859710:intron 25.0%
! TATAGTTTATTTGCATGTAC+AGG + chr5_4:58165438-58165457 Msa0859710:intron 25.0%
! TTATCAAAAGCAAAGAACTT+TGG + chr5_4:58165578-58165597 Msa0859710:CDS 25.0%
!!! TTTTTTATTTCTCAAAGGTG+TGG + chr5_4:58165722-58165741 Msa0859710:intron 25.0%
! TCAGTTTATATTATTCTGAC+AGG + chr5_4:58166131-58166150 Msa0859710:intron 25.0%
! GTACTTACATGGAAATATAA+TGG + chr5_4:58166826-58166845 Msa0859710:intron 25.0%
! AAAAGATGGAGAAATAATTG+AGG + chr5_4:58167300-58167319 Msa0859710:CDS 25.0%
TAGATTGATGAATGAATCTG+CGG - chr5_4:58162427-58162446 None:intergenic 30.0%
! TTTTATGATTCTGGGTTTTG+CGG + chr5_4:58162771-58162790 Msa0859710:intron 30.0%
TTCCATGTAAACAAATGTGT+CGG - chr5_4:58163037-58163056 None:intergenic 30.0%
TACACAACATATATGAAGCA+CGG - chr5_4:58163126-58163145 None:intergenic 30.0%
!! TACACCTCTAGAATCAATTT+CGG + chr5_4:58163398-58163417 Msa0859710:intron 30.0%
ATCATACGTGTGAATATAGA+AGG - chr5_4:58163494-58163513 None:intergenic 30.0%
AACTCAAATTAAGGTCTGTT+TGG + chr5_4:58163554-58163573 Msa0859710:intron 30.0%
AATAAGAGGAAAACACCTTA+TGG - chr5_4:58163662-58163681 None:intergenic 30.0%
GAAGAGCTTATGAAAATAAG+AGG - chr5_4:58163676-58163695 None:intergenic 30.0%
! TATTCATATTTTCTTGGGGA+GGG - chr5_4:58163855-58163874 None:intergenic 30.0%
! TTATTCATATTTTCTTGGGG+AGG - chr5_4:58163856-58163875 None:intergenic 30.0%
CTAGAAATACATACCTTTGA+TGG + chr5_4:58164278-58164297 Msa0859710:intron 30.0%
TCGAATAAACCAATCTAAAC+AGG - chr5_4:58164334-58164353 None:intergenic 30.0%
TATTGACATAAGTACCTGTT+AGG + chr5_4:58164360-58164379 Msa0859710:intron 30.0%
ATTGACATAAGTACCTGTTA+GGG + chr5_4:58164361-58164380 Msa0859710:intron 30.0%
TTTCATAAACTTTCCCTAAC+AGG - chr5_4:58164377-58164396 None:intergenic 30.0%
! TTGGTTGCTTTACATAGAAA+GGG - chr5_4:58164754-58164773 None:intergenic 30.0%
! TTTGGTTGCTTTACATAGAA+AGG - chr5_4:58164755-58164774 None:intergenic 30.0%
CCTGTTATGATTTACATGTT+CGG + chr5_4:58164898-58164917 Msa0859710:intron 30.0%
TCTAATACCATATTCCTGAA+GGG - chr5_4:58165008-58165027 None:intergenic 30.0%
TAGAGGATTTCCAACTATAA+AGG + chr5_4:58165024-58165043 Msa0859710:CDS 30.0%
! TTTGATTGAAACTTTAGGGA+GGG - chr5_4:58165165-58165184 None:intergenic 30.0%
! ATTTGATTGAAACTTTAGGG+AGG - chr5_4:58165166-58165185 None:intergenic 30.0%
AATTGTAACACTACACATGT+TGG - chr5_4:58165193-58165212 None:intergenic 30.0%
ACATGTGTAGTGTTACAATT+TGG + chr5_4:58165193-58165212 Msa0859710:intron 30.0%
! AGATAGATGTCTTCTTTTTG+TGG + chr5_4:58165230-58165249 Msa0859710:intron 30.0%
GAGTGAGAATATCAATGTAT+TGG + chr5_4:58165369-58165388 Msa0859710:intron 30.0%
TTATTTGCATGTACAGGTAA+AGG + chr5_4:58165444-58165463 Msa0859710:intron 30.0%
TAGAATTACATTGTTCAGTC+AGG - chr5_4:58165659-58165678 None:intergenic 30.0%
!!! CAGGAGCTAATTTTTTACAA+TGG - chr5_4:58165748-58165767 None:intergenic 30.0%
! AGAGCATCCAATAATTTGAA+AGG + chr5_4:58165776-58165795 Msa0859710:CDS 30.0%
GAGCATCCAATAATTTGAAA+GGG + chr5_4:58165777-58165796 Msa0859710:CDS 30.0%
ATCCAATAATTTGAAAGGGA+AGG + chr5_4:58165781-58165800 Msa0859710:CDS 30.0%
AACCTTCCCTTTCAAATTAT+TGG - chr5_4:58165786-58165805 None:intergenic 30.0%
ATGCAGATAGCAAAAAATAG+CGG - chr5_4:58166031-58166050 None:intergenic 30.0%
! AGGAATTGGAGTATCTTTAT+CGG - chr5_4:58166231-58166250 None:intergenic 30.0%
TACAATCAAGTAAAGTTTGC+AGG - chr5_4:58166368-58166387 None:intergenic 30.0%
! TAAACATGAGTGTTGTCTAT+AGG + chr5_4:58166417-58166436 Msa0859710:intron 30.0%
!!! TGTTTTTTTACTTGCCTGTA+AGG - chr5_4:58166580-58166599 None:intergenic 30.0%
ATTAACTCATGAGTCATAGA+GGG - chr5_4:58166637-58166656 None:intergenic 30.0%
!! GAAATATAATGGGTTCTCAT+TGG + chr5_4:58166837-58166856 Msa0859710:intron 30.0%
! ATTTTCTCTTGAAGAACTCA+TGG + chr5_4:58167330-58167349 Msa0859710:CDS 30.0%
! TTTTCTCTTGAAGAACTCAT+GGG + chr5_4:58167331-58167350 Msa0859710:CDS 30.0%
!! CATTACCAGTTTTATGCTAT+AGG + chr5_4:58167425-58167444 Msa0859710:three_prime_UTR 30.0%
GAATATTAAAGGGAAATGTG+TGG + chr5_4:58167463-58167482 Msa0859710:three_prime_UTR 30.0%
TCGTATTAAGGTGAATAAGT+GGG + chr5_4:58167800-58167819 Msa0859710:three_prime_UTR 30.0%
! TTAGTGAAGTGTTTAGAAGA+TGG + chr5_4:58167936-58167955 Msa0859710:three_prime_UTR 30.0%
GTTCTTGTATGAAGAGAAGA+AGG - chr5_4:58162390-58162409 None:intergenic 35.0%
AGATTGATGAATGAATCTGC+GGG - chr5_4:58162426-58162445 None:intergenic 35.0%
AATCTACGCTTCTTCAACAA+TGG + chr5_4:58162441-58162460 Msa0859710:exon 35.0%
! TTTGCTATGTTGCTGTATTG+TGG + chr5_4:58162702-58162721 Msa0859710:intron 35.0%
TGTAGTGTAGTGTGTGAATA+TGG + chr5_4:58162744-58162763 Msa0859710:intron 35.0%
GGCAAAATCAATTCTACTCT+AGG + chr5_4:58163349-58163368 Msa0859710:intron 35.0%
! ATTCATATTTTCTTGGGGAG+GGG - chr5_4:58163854-58163873 None:intergenic 35.0%
!! GGTGCTATTTGAATAATGAC+AGG + chr5_4:58163935-58163954 Msa0859710:intron 35.0%
!! TGACAGGTTCTGAATTCAAA+TGG + chr5_4:58163951-58163970 Msa0859710:intron 35.0%
TTGTTGAATTCTTTGCTCCA+TGG + chr5_4:58163982-58164001 Msa0859710:CDS 35.0%
AAGGCAGCTACTGTATTAAA+GGG + chr5_4:58164041-58164060 Msa0859710:CDS 35.0%
AGGAATGAAGAAATGAGCAA+TGG - chr5_4:58164124-58164143 None:intergenic 35.0%
! GGTATTGAACCTGTTTAGAT+TGG + chr5_4:58164322-58164341 Msa0859710:intron 35.0%
AAGTTATCCTAGAGAGCTTA+TGG - chr5_4:58164439-58164458 None:intergenic 35.0%
CATATGAAATATGTCGAGCA+TGG + chr5_4:58164618-58164637 Msa0859710:intron 35.0%
TTACATAGAAAGGGAAGACT+AGG - chr5_4:58164745-58164764 None:intergenic 35.0%
ACAAATGTCATGTAAGAGAC+AGG - chr5_4:58164829-58164848 None:intergenic 35.0%
CCGAACATGTAAATCATAAC+AGG - chr5_4:58164901-58164920 None:intergenic 35.0%
! GTCTTTTCTTAATTCCCTTC+AGG + chr5_4:58164991-58165010 Msa0859710:intron 35.0%
CTTAATTCCCTTCAGGAATA+TGG + chr5_4:58164998-58165017 Msa0859710:intron 35.0%
CTCTAATACCATATTCCTGA+AGG - chr5_4:58165009-58165028 None:intergenic 35.0%
CTTCAGGAATATGGTATTAG+AGG + chr5_4:58165007-58165026 Msa0859710:intron 35.0%
!! TAAAGGCTCTTTTGAAGGAA+CGG + chr5_4:58165461-58165480 Msa0859710:CDS 35.0%
!! CTTTTGAAGGAACGGTTAAA+TGG + chr5_4:58165469-58165488 Msa0859710:CDS 35.0%
TTAAATGGAAAAGCAACAGG+TGG + chr5_4:58165484-58165503 Msa0859710:CDS 35.0%
CTCTAGTAACTTCGATGAAT+TGG + chr5_4:58165555-58165574 Msa0859710:CDS 35.0%
!!! TTGTAGATTTTTTCGCACCT+TGG + chr5_4:58165602-58165621 Msa0859710:CDS 35.0%
!! AATATAGGAAGTACTCACCA+AGG - chr5_4:58165622-58165641 None:intergenic 35.0%
GTAAAAAATTAGCTCCTGAG+TGG + chr5_4:58165750-58165769 Msa0859710:CDS 35.0%
TTTGAAAGGGAAGGTTAAAC+TGG + chr5_4:58165790-58165809 Msa0859710:CDS 35.0%
TTGAAAGGGAAGGTTAAACT+GGG + chr5_4:58165791-58165810 Msa0859710:CDS 35.0%
TTCCCAACAATCTTGGTATT+TGG + chr5_4:58166203-58166222 Msa0859710:CDS 35.0%
CTCCAAATACCAAGATTGTT+GGG - chr5_4:58166208-58166227 None:intergenic 35.0%
! GTGTTGTCTATAGGATGTTT+TGG + chr5_4:58166426-58166445 Msa0859710:intron 35.0%
!!! GATGTTTTGGAAGAGAAATG+TGG + chr5_4:58166439-58166458 Msa0859710:CDS 35.0%
CAACTGTTAATAACTGCTGA+AGG - chr5_4:58166537-58166556 None:intergenic 35.0%
GATTAACTCATGAGTCATAG+AGG - chr5_4:58166638-58166657 None:intergenic 35.0%
GTTTGATGAAGATGCATTGT+TGG - chr5_4:58166887-58166906 None:intergenic 35.0%
CTTTGTTGACGAATGCTTAA+TGG + chr5_4:58166919-58166938 Msa0859710:intron 35.0%
AACACCTTTCTTAACGTTCA+AGG - chr5_4:58167057-58167076 None:intergenic 35.0%
AGAGAATTTGTGAAAGAAGC+TGG + chr5_4:58167205-58167224 Msa0859710:intron 35.0%
CATTGTAAAGACAGAACCAT+GGG + chr5_4:58167273-58167292 Msa0859710:CDS 35.0%
CATCTCCTATAGCATAAAAC+TGG - chr5_4:58167433-58167452 None:intergenic 35.0%
GAAAAATCTGAAAAACAGCG+AGG - chr5_4:58167505-58167524 None:intergenic 35.0%
TTCATGTCATTCTCATTCAG+TGG + chr5_4:58167574-58167593 Msa0859710:three_prime_UTR 35.0%
AACATACCTCAGGAAAATGT+TGG + chr5_4:58167600-58167619 Msa0859710:three_prime_UTR 35.0%
AACATTATTACGAGAAGACC+TGG + chr5_4:58167666-58167685 Msa0859710:three_prime_UTR 35.0%
CTCGTATTAAGGTGAATAAG+TGG + chr5_4:58167799-58167818 Msa0859710:three_prime_UTR 35.0%
AACACTTCACTAATGCATGT+AGG - chr5_4:58167929-58167948 None:intergenic 35.0%
ATTGATGAATGAATCTGCGG+GGG - chr5_4:58162424-58162443 None:intergenic 40.0%
GATTGATGAATGAATCTGCG+GGG - chr5_4:58162425-58162444 None:intergenic 40.0%
ATCCTTTCTCATGCTCTCTA+CGG + chr5_4:58162511-58162530 Msa0859710:CDS 40.0%
! TTAGGAGTGAGTTGAAGAAC+GGG - chr5_4:58162547-58162566 None:intergenic 40.0%
! GTTAGGAGTGAGTTGAAGAA+CGG - chr5_4:58162548-58162567 None:intergenic 40.0%
TCCTAACAACTTCAAGTCCA+AGG + chr5_4:58162561-58162580 Msa0859710:CDS 40.0%
ACCTTGGACTTGAAGTTGTT+AGG - chr5_4:58162565-58162584 None:intergenic 40.0%
AAAAAGTGGGATCACGAACT+GGG - chr5_4:58162615-58162634 None:intergenic 40.0%
AAAAAAGTGGGATCACGAAC+TGG - chr5_4:58162616-58162635 None:intergenic 40.0%
!! TATGAAGCACTGACACTCTT+AGG + chr5_4:58162964-58162983 Msa0859710:intron 40.0%
TCTTAGGATTAGGCATGTCT+GGG + chr5_4:58162980-58162999 Msa0859710:intron 40.0%
CACCGACACATTTGTTTACA+TGG + chr5_4:58163032-58163051 Msa0859710:intron 40.0%
GTTGTGTATGTTTCGTTCTG+TGG + chr5_4:58163138-58163157 Msa0859710:intron 40.0%
TTGCTTATGACGTGTCCATA+AGG + chr5_4:58163644-58163663 Msa0859710:intron 40.0%
! TAAGGCTCTGACTCCTATAT+GGG + chr5_4:58164016-58164035 Msa0859710:CDS 40.0%
GAAGGCAGCTACTGTATTAA+AGG + chr5_4:58164040-58164059 Msa0859710:CDS 40.0%
ACCACACACAATGCAAAACA+TGG + chr5_4:58164301-58164320 Msa0859710:intron 40.0%
! ACCATGTTTTGCATTGTGTG+TGG - chr5_4:58164305-58164324 None:intergenic 40.0%
! GCTATTTCCATAAGCTCTCT+AGG + chr5_4:58164429-58164448 Msa0859710:intron 40.0%
TGAAATATGTCGAGCATGGT+AGG + chr5_4:58164622-58164641 Msa0859710:intron 40.0%
GAAGACTAGGAATGTAAGGA+AGG - chr5_4:58164732-58164751 None:intergenic 40.0%
AAGGGAAGACTAGGAATGTA+AGG - chr5_4:58164736-58164755 None:intergenic 40.0%
GGTGCAAACACCTTTATAGT+TGG - chr5_4:58165037-58165056 None:intergenic 40.0%
!! ACTATAAAGGTGTTTGCACC+TGG + chr5_4:58165037-58165056 Msa0859710:CDS 40.0%
!! TGCGATTGGTTTAACATCTC+TGG - chr5_4:58165089-58165108 None:intergenic 40.0%
TGAAGTGCAAATTCTGCGAT+TGG - chr5_4:58165103-58165122 None:intergenic 40.0%
TTTGCACTTCAACAGGTTTG+TGG + chr5_4:58165112-58165131 Msa0859710:intron 40.0%
AGGTTTGTGGAATCTCTTCT+TGG + chr5_4:58165125-58165144 Msa0859710:intron 40.0%
ACTCTTCTTGCAACATCACT+TGG + chr5_4:58165326-58165345 Msa0859710:intron 40.0%
! ACAGGTAAAGGCTCTTTTGA+AGG + chr5_4:58165456-58165475 Msa0859710:intron 40.0%
CGGTTAAATGGAAAAGCAAC+AGG + chr5_4:58165481-58165500 Msa0859710:CDS 40.0%
! TAGTTCTACCGAAGAAGAAG+CGG - chr5_4:58165533-58165552 None:intergenic 40.0%
ATGCTGACAAGGTAAGCTTA+TGG + chr5_4:58165828-58165847 Msa0859710:intron 40.0%
TGCTATAGTGCTTCTATAGC+TGG - chr5_4:58165987-58166006 None:intergenic 40.0%
TTTGTCAGTCTCTAATGAGC+AGG + chr5_4:58166165-58166184 Msa0859710:intron 40.0%
ATGAGCAGGTTCAATGTTCA+AGG + chr5_4:58166179-58166198 Msa0859710:CDS 40.0%
TCAAGGATTCCCAACAATCT+TGG + chr5_4:58166196-58166215 Msa0859710:CDS 40.0%
GCTCCAAATACCAAGATTGT+TGG - chr5_4:58166209-58166228 None:intergenic 40.0%
AGATACTCCAATTCCTTACG+AGG + chr5_4:58166235-58166254 Msa0859710:CDS 40.0%
GATACTCCAATTCCTTACGA+GGG + chr5_4:58166236-58166255 Msa0859710:CDS 40.0%
! CTATATAGCTCTGTCACTTC+TGG - chr5_4:58166326-58166345 None:intergenic 40.0%
CAGTTGCAGAGAAGTTCAAA+AGG + chr5_4:58166551-58166570 Msa0859710:CDS 40.0%
AGTTCAAAAGGAGTCCTTAC+AGG + chr5_4:58166563-58166582 Msa0859710:CDS 40.0%
GGTTGCAAAGTGTACTTACA+TGG + chr5_4:58166815-58166834 Msa0859710:intron 40.0%
GATGCATTGTTGGTGCAAAA+TGG - chr5_4:58166877-58166896 None:intergenic 40.0%
GGTCTTGAACAGCTATGTAT+GGG + chr5_4:58166959-58166978 Msa0859710:intron 40.0%
! GTAGCCTTGAACGTTAAGAA+AGG + chr5_4:58167050-58167069 Msa0859710:CDS 40.0%
!! AGTTCAAAAGCACTCTTGAG+AGG - chr5_4:58167086-58167105 None:intergenic 40.0%
GGTTCTGTCTTTACAATGGT+GGG - chr5_4:58167271-58167290 None:intergenic 40.0%
! TGGTTCTGTCTTTACAATGG+TGG - chr5_4:58167272-58167291 None:intergenic 40.0%
! CCATGGTTCTGTCTTTACAA+TGG - chr5_4:58167275-58167294 None:intergenic 40.0%
CCATTGTAAAGACAGAACCA+TGG + chr5_4:58167272-58167291 Msa0859710:CDS 40.0%
AAGACGAGTATAGTGATGAG+TGG + chr5_4:58167538-58167557 Msa0859710:three_prime_UTR 40.0%
! TAGTGATGAGTGGTCTTTTG+AGG + chr5_4:58167548-58167567 Msa0859710:three_prime_UTR 40.0%
! AACATGCCAACATTTTCCTG+AGG - chr5_4:58167609-58167628 None:intergenic 40.0%
TGTTGAATGTTGCACACAAC+CGG - chr5_4:58167721-58167740 None:intergenic 40.0%
GTGGCTTGAAACTCGTATTA+AGG + chr5_4:58167788-58167807 Msa0859710:three_prime_UTR 40.0%
TCGAACGTTGCATCATAAAG+GGG - chr5_4:58167847-58167866 None:intergenic 40.0%
CTCGAACGTTGCATCATAAA+GGG - chr5_4:58167848-58167867 None:intergenic 40.0%
CAACGTTCGAGCTATGTTTA+CGG + chr5_4:58167857-58167876 Msa0859710:three_prime_UTR 40.0%
! AACCGTAGAGAGCATGAGAA+AGG - chr5_4:58162516-58162535 None:intergenic 45.0%
! GCACTGACACTCTTAGGATT+AGG + chr5_4:58162970-58162989 Msa0859710:intron 45.0%
CTCTTAGGATTAGGCATGTC+TGG + chr5_4:58162979-58162998 Msa0859710:intron 45.0%
!! CAAATGTGTCGGTGTCATGT+CGG - chr5_4:58163026-58163045 None:intergenic 45.0%
! GGAGCCGAAATTGATTCTAG+AGG - chr5_4:58163405-58163424 None:intergenic 45.0%
GAATTCTTTGCTCCATGGTG+CGG + chr5_4:58163987-58164006 Msa0859710:CDS 45.0%
AATTCTTTGCTCCATGGTGC+GGG + chr5_4:58163988-58164007 Msa0859710:CDS 45.0%
! GTAAGGCTCTGACTCCTATA+TGG + chr5_4:58164015-58164034 Msa0859710:CDS 45.0%
TCTGACTCCTATATGGGAGA+AGG + chr5_4:58164022-58164041 Msa0859710:CDS 45.0%
CATTGTGTGTGGTCCATCAA+AGG - chr5_4:58164294-58164313 None:intergenic 45.0%
AGAGGACACTTGACCCTATT+TGG - chr5_4:58164576-58164595 None:intergenic 45.0%
! TAATCGACTGGTGGTTTTCC+AGG - chr5_4:58165058-58165077 None:intergenic 45.0%
AAACCACCAGTCGATTACCA+AGG + chr5_4:58165061-58165080 Msa0859710:CDS 45.0%
! GTTTAACATCTCTGGCTCCT+TGG - chr5_4:58165081-58165100 None:intergenic 45.0%
CGCAGAATTTGCACTTCAAC+AGG + chr5_4:58165105-58165124 Msa0859710:CDS 45.0%
AGAAACCACCGCTTCTTCTT+CGG + chr5_4:58165522-58165541 Msa0859710:CDS 45.0%
! TTGGATGCTCTTTTCCACTC+AGG - chr5_4:58165767-58165786 None:intergenic 45.0%
! TGTTGACTGTGATGCTGACA+AGG + chr5_4:58165817-58165836 Msa0859710:CDS 45.0%
! GAAGCACTATAGCACTGTAG+TGG + chr5_4:58165993-58166012 Msa0859710:intron 45.0%
CTCTAGTGCGAATGATTCGA+TGG - chr5_4:58166279-58166298 None:intergenic 45.0%
! TATAGCTCTGTCACTTCTGG+AGG - chr5_4:58166323-58166342 None:intergenic 45.0%
! TTCCTTCCTTCCCGACATTT+TGG + chr5_4:58166483-58166502 Msa0859710:CDS 45.0%
ATCCAAAATGTCGGGAAGGA+AGG - chr5_4:58166488-58166507 None:intergenic 45.0%
TGGAATCCAAAATGTCGGGA+AGG - chr5_4:58166492-58166511 None:intergenic 45.0%
! TCCCGACATTTTGGATTCCA+AGG + chr5_4:58166492-58166511 Msa0859710:CDS 45.0%
GCCTTGGAATCCAAAATGTC+GGG - chr5_4:58166496-58166515 None:intergenic 45.0%
CGCCTTGGAATCCAAAATGT+CGG - chr5_4:58166497-58166516 None:intergenic 45.0%
! CGACATTTTGGATTCCAAGG+CGG + chr5_4:58166495-58166514 Msa0859710:CDS 45.0%
! ATTTTGGATTCCAAGGCGGA+GGG + chr5_4:58166499-58166518 Msa0859710:CDS 45.0%
GGGTCTTGAACAGCTATGTA+TGG + chr5_4:58166958-58166977 Msa0859710:intron 45.0%
CAACACCAACTTTCTGCTCA+AGG - chr5_4:58167007-58167026 None:intergenic 45.0%
!! CTTGAGCAGAAAGTTGGTGT+TGG + chr5_4:58167005-58167024 Msa0859710:CDS 45.0%
!! AGCAGAAAGTTGGTGTTGGT+GGG + chr5_4:58167009-58167028 Msa0859710:CDS 45.0%
!! AAAGTTGGTGTTGGTGGGTA+TGG + chr5_4:58167014-58167033 Msa0859710:CDS 45.0%
ACGTTCAAGGCTACTAATGC+TGG - chr5_4:58167044-58167063 None:intergenic 45.0%
TTTGTGAAAGAAGCTGGACG+TGG + chr5_4:58167211-58167230 Msa0859710:CDS 45.0%
! TCTGTCTTTACAATGGTGGG+AGG - chr5_4:58167268-58167287 None:intergenic 45.0%
GTAAAGACAGAACCATGGGA+TGG + chr5_4:58167277-58167296 Msa0859710:CDS 45.0%
GAACCATGGGATGGAAAAGA+TGG + chr5_4:58167286-58167305 Msa0859710:CDS 45.0%
! TCTCCATCTTTTCCATCCCA+TGG - chr5_4:58167292-58167311 None:intergenic 45.0%
TCAGTGGTACAACATACCTC+AGG + chr5_4:58167590-58167609 Msa0859710:three_prime_UTR 45.0%
! TACGAGAAGACCTGGTTCTT+TGG + chr5_4:58167674-58167693 Msa0859710:three_prime_UTR 45.0%
GCTCGAACGTTGCATCATAA+AGG - chr5_4:58167849-58167868 None:intergenic 45.0%
GAATCTGCGGGGGATGAAAA+TGG - chr5_4:58162414-58162433 None:intergenic 50.0%
! AGTGAGTTGAAGAACGGGAG+AGG - chr5_4:58162542-58162561 None:intergenic 50.0%
ACGACATGGACACTGACATG+TGG - chr5_4:58163091-58163110 None:intergenic 50.0%
AGTGTCCATGTCGTCGTATC+AGG + chr5_4:58163097-58163116 Msa0859710:intron 50.0%
GTAGCTGCCTTCTCCCATAT+AGG - chr5_4:58164032-58164051 None:intergenic 50.0%
TGCTGATGCTCACCAATCTC+TGG + chr5_4:58164088-58164107 Msa0859710:CDS 50.0%
GGAAAACCTGAGCCAGAGAT+TGG - chr5_4:58164103-58164122 None:intergenic 50.0%
TTCTACCGAAGAAGAAGCGG+TGG - chr5_4:58165530-58165549 None:intergenic 50.0%
CTAGCACCCTCGTAAGGAAT+TGG - chr5_4:58166245-58166264 None:intergenic 50.0%
ATCATTCGCACTAGAGCAGC+TGG + chr5_4:58166283-58166302 Msa0859710:CDS 50.0%
GCAGGAGAACAAACACTCAC+TGG - chr5_4:58166350-58166369 None:intergenic 50.0%
! CATTTTGGATTCCAAGGCGG+AGG + chr5_4:58166498-58166517 Msa0859710:CDS 50.0%
! TTTTGGATTCCAAGGCGGAG+GGG + chr5_4:58166500-58166519 Msa0859710:CDS 50.0%
CCAACTTTCTGCTCAAGGTC+TGG - chr5_4:58167002-58167021 None:intergenic 50.0%
CCAGACCTTGAGCAGAAAGT+TGG + chr5_4:58166999-58167018 Msa0859710:CDS 50.0%
!! GAGCAGAAAGTTGGTGTTGG+TGG + chr5_4:58167008-58167027 Msa0859710:CDS 50.0%
GAGGAACGATCCAAAGAACC+AGG - chr5_4:58167687-58167706 None:intergenic 50.0%
CGTTCCTCATTGACCATAGC+CGG + chr5_4:58167699-58167718 Msa0859710:three_prime_UTR 50.0%
ACAACCGGCTATGGTCAATG+AGG - chr5_4:58167706-58167725 None:intergenic 50.0%
ATGTTGCACACAACCGGCTA+TGG - chr5_4:58167715-58167734 None:intergenic 50.0%
TGTCGGACACAGACGCATGT+CGG - chr5_4:58163009-58163028 None:intergenic 55.0%
GGACACCTGATACGACGACA+TGG - chr5_4:58163105-58163124 None:intergenic 55.0%
! TGCTCACCAATCTCTGGCTC+AGG + chr5_4:58164094-58164113 Msa0859710:CDS 55.0%
! GCTCCTTGGTAATCGACTGG+TGG - chr5_4:58165067-58165086 None:intergenic 55.0%
! CTGGCTCCTTGGTAATCGAC+TGG - chr5_4:58165070-58165089 None:intergenic 55.0%
! GCAGTTCTAGCACCCTCGTA+AGG - chr5_4:58166251-58166270 None:intergenic 55.0%
AGCTATGTATGGGCAGCTGC+CGG + chr5_4:58166969-58166988 Msa0859710:intron 55.0%
CTCAAGGTCTGGCTGCTTAC+CGG - chr5_4:58166991-58167010 None:intergenic 55.0%
GTGAAAGAAGCTGGACGTGG+AGG + chr5_4:58167214-58167233 Msa0859710:CDS 55.0%
AAGCTGGACGTGGAGGCAAA+GGG + chr5_4:58167221-58167240 Msa0859710:CDS 55.0%
! TCCATGGTGCGGGCACTGTA+AGG + chr5_4:58163998-58164017 Msa0859710:CDS 60.0%
! TCTGTTTCTCCCCTCCGCCT+TGG - chr5_4:58166512-58166531 None:intergenic 60.0%
GAAGCTGGACGTGGAGGCAA+AGG + chr5_4:58167220-58167239 Msa0859710:CDS 60.0%
GGCAAAGGGAACTTGCCCCT+AGG + chr5_4:58167235-58167254 Msa0859710:CDS 60.0%
!! GTGGGAGGAGTGTCACCTAG+GGG - chr5_4:58167253-58167272 None:intergenic 60.0%
!! GGTGGGAGGAGTGTCACCTA+GGG - chr5_4:58167254-58167273 None:intergenic 60.0%
!! TGGTGGGAGGAGTGTCACCT+AGG - chr5_4:58167255-58167274 None:intergenic 60.0%
GCCTTACAGTGCCCGCACCA+TGG - chr5_4:58164002-58164021 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 58162374 58168017 58162374 ID=Msa0859710;Name=Msa0859710
chr5_4 mRNA 58162374 58168017 58162374 ID=Msa0859710-mRNA-1;Parent=Msa0859710;Name=Msa0859710-mRNA-1;_AED=0.12;_eAED=0.12;_QI=86|1|1|1|1|1|9|633|435
chr5_4 exon 58162374 58162582 58162374 ID=Msa0859710-mRNA-1:exon:17691;Parent=Msa0859710-mRNA-1
chr5_4 exon 58163957 58164115 58163957 ID=Msa0859710-mRNA-1:exon:17692;Parent=Msa0859710-mRNA-1
chr5_4 exon 58165013 58165126 58165013 ID=Msa0859710-mRNA-1:exon:17693;Parent=Msa0859710-mRNA-1
chr5_4 exon 58165460 58165623 58165460 ID=Msa0859710-mRNA-1:exon:17694;Parent=Msa0859710-mRNA-1
chr5_4 exon 58165739 58165838 58165739 ID=Msa0859710-mRNA-1:exon:17695;Parent=Msa0859710-mRNA-1
chr5_4 exon 58166173 58166349 58166173 ID=Msa0859710-mRNA-1:exon:17696;Parent=Msa0859710-mRNA-1
chr5_4 exon 58166439 58166584 58166439 ID=Msa0859710-mRNA-1:exon:17697;Parent=Msa0859710-mRNA-1
chr5_4 exon 58166971 58167117 58166971 ID=Msa0859710-mRNA-1:exon:17698;Parent=Msa0859710-mRNA-1
chr5_4 exon 58167207 58168017 58167207 ID=Msa0859710-mRNA-1:exon:17699;Parent=Msa0859710-mRNA-1
chr5_4 five_prime_UTR 58162374 58162459 58162374 ID=Msa0859710-mRNA-1:five_prime_utr;Parent=Msa0859710-mRNA-1
chr5_4 CDS 58162460 58162582 58162460 ID=Msa0859710-mRNA-1:cds;Parent=Msa0859710-mRNA-1
chr5_4 CDS 58163957 58164115 58163957 ID=Msa0859710-mRNA-1:cds;Parent=Msa0859710-mRNA-1
chr5_4 CDS 58165013 58165126 58165013 ID=Msa0859710-mRNA-1:cds;Parent=Msa0859710-mRNA-1
chr5_4 CDS 58165460 58165623 58165460 ID=Msa0859710-mRNA-1:cds;Parent=Msa0859710-mRNA-1
chr5_4 CDS 58165739 58165838 58165739 ID=Msa0859710-mRNA-1:cds;Parent=Msa0859710-mRNA-1
chr5_4 CDS 58166173 58166349 58166173 ID=Msa0859710-mRNA-1:cds;Parent=Msa0859710-mRNA-1
chr5_4 CDS 58166439 58166584 58166439 ID=Msa0859710-mRNA-1:cds;Parent=Msa0859710-mRNA-1
chr5_4 CDS 58166971 58167117 58166971 ID=Msa0859710-mRNA-1:cds;Parent=Msa0859710-mRNA-1
chr5_4 CDS 58167207 58167384 58167207 ID=Msa0859710-mRNA-1:cds;Parent=Msa0859710-mRNA-1
chr5_4 three_prime_UTR 58167385 58168017 58167385 ID=Msa0859710-mRNA-1:three_prime_utr;Parent=Msa0859710-mRNA-1
Gene Sequence

>Msa0859710

ATGGCGAAACTCTCATTCATTGCTGTAACTATCTTTCTCTTCAACAATCTCATCCTTTCTCATGCTCTCTACGGTTCTTCCTCTCCCGTTCTTCAACTCACTCCTAACAACTTCAAGTCCAAGGTTCTGAATTCAAATGGAGTTGTTCTTGTTGAATTCTTTGCTCCATGGTGCGGGCACTGTAAGGCTCTGACTCCTATATGGGAGAAGGCAGCTACTGTATTAAAGGGTGTAGTCACTGTAGCAGCACTTGATGCTGATGCTCACCAATCTCTGGCTCAGGAATATGGTATTAGAGGATTTCCAACTATAAAGGTGTTTGCACCTGGAAAACCACCAGTCGATTACCAAGGAGCCAGAGATGTTAAACCAATCGCAGAATTTGCACTTCAACAGGTAAAGGCTCTTTTGAAGGAACGGTTAAATGGAAAAGCAACAGGTGGATCAAATGAAAAAAAAGAAACCACCGCTTCTTCTTCGGTAGAACTAAACTCTAGTAACTTCGATGAATTGGTTATCAAAAGCAAAGAACTTTGGATTGTAGATTTTTTCGCACCTTGGTGTGGCCATTGTAAAAAATTAGCTCCTGAGTGGAAAAGAGCATCCAATAATTTGAAAGGGAAGGTTAAACTGGGTCATGTTGACTGTGATGCTGACAAGTCTCTAATGAGCAGGTTCAATGTTCAAGGATTCCCAACAATCTTGGTATTTGGAGCCGATAAAGATACTCCAATTCCTTACGAGGGTGCTAGAACTGCATCAGCCATCGAATCATTCGCACTAGAGCAGCTGGAAACAAATGTTGCTCCTCCAGAAGTGACAGAGCTATATAGTCCAGATGTTTTGGAAGAGAAATGTGGTTCTGCTGCTATCTGTTTTGTTTCCTTCCTTCCCGACATTTTGGATTCCAAGGCGGAGGGGAGAAACAGATACCTTCAGCAGTTATTAACAGTTGCAGAGAAGTTCAAAAGGAGTCCTTACAGCTATGTATGGGCAGCTGCCGGTAAGCAGCCAGACCTTGAGCAGAAAGTTGGTGTTGGTGGGTATGGTTATCCAGCATTAGTAGCCTTGAACGTTAAGAAAGGTGTTTATGCTCCTCTCAAGAGTGCTTTTGAACTTGACCAAATTATAGAATTTGTGAAAGAAGCTGGACGTGGAGGCAAAGGGAACTTGCCCCTAGGTGACACTCCTCCCACCATTGTAAAGACAGAACCATGGGATGGAAAAGATGGAGAAATAATTGAGGAAGATGAATTTTCTCTTGAAGAACTCATGGGAGAAGATGCCTCAACCAAAGATGAACTATAA
Protein sequence

>Msa0859710

MAKLSFIAVTIFLFNNLILSHALYGSSSPVLQLTPNNFKSKVLNSNGVVLVEFFAPWCGHCKALTPIWEKAATVLKGVVTVAALDADAHQSLAQEYGIRGFPTIKVFAPGKPPVDYQGARDVKPIAEFALQQVKALLKERLNGKATGGSNEKKETTASSSVELNSSNFDELVIKSKELWIVDFFAPWCGHCKKLAPEWKRASNNLKGKVKLGHVDCDADKSLMSRFNVQGFPTILVFGADKDTPIPYEGARTASAIESFALEQLETNVAPPEVTELYSPDVLEEKCGSAAICFVSFLPDILDSKAEGRNRYLQQLLTVAEKFKRSPYSYVWAAAGKQPDLEQKVGVGGYGYPALVALNVKKGVYAPLKSAFELDQIIEFVKEAGRGGKGNLPLGDTPPTIVKTEPWDGKDGEIIEEDEFSLEELMGEDASTKDEL*