AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0739080

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0739080 MtrunA17_Chr5g0438731 98.853 436 4 1 1 435 32 467 0.0 880
Msa0739080 MtrunA17_Chr2g0287471 84.962 133 20 0 1 133 1 133 4.04e-73 233
Msa0739080 MtrunA17_Chr2g0287471 44.037 109 60 1 156 264 26 133 1.58e-23 100
Msa0739080 MtrunA17_Chr7g0271721 34.074 270 152 8 1 267 3 249 7.33e-40 146
Msa0739080 MtrunA17_Chr7g0271721 42.308 130 67 4 157 280 23 150 1.91e-21 95.1
Msa0739080 MtrunA17_Chr1g0194961 35.000 240 136 5 33 267 34 258 8.60e-40 146
Msa0739080 MtrunA17_Chr1g0194961 40.541 148 70 4 28 172 147 279 1.40e-24 104
Msa0739080 MtrunA17_Chr1g0194961 40.625 128 68 4 163 284 34 159 2.15e-19 89.4
Msa0739080 MtrunA17_Chr1g0200731 40.351 114 59 4 30 136 95 206 7.88e-17 82.4
Msa0739080 MtrunA17_Chr1g0200731 28.700 223 135 5 163 382 98 299 6.37e-15 76.6
Msa0739080 MtrunA17_Chr3g0124851 34.884 172 93 7 163 329 38 195 1.13e-16 82.0
Msa0739080 MtrunA17_Chr3g0124851 41.284 109 57 4 30 132 35 142 1.21e-16 82.0
Msa0739080 MtrunA17_Chr3g0124851 33.333 105 68 1 26 128 375 479 7.82e-12 67.0
Msa0739080 MtrunA17_Chr2g0326581 41.600 125 65 4 135 256 55 174 1.29e-14 75.9
Msa0739080 MtrunA17_Chr2g0326581 51.220 82 38 2 30 110 80 160 2.87e-13 71.6
Msa0739080 MtrunA17_Chr2g0326581 27.184 206 132 6 64 259 322 519 1.08e-11 66.6
Msa0739080 MtrunA17_Chr2g0326581 32.609 138 87 3 36 169 424 559 1.33e-11 66.6
Msa0739080 MtrunA17_Chr8g0360031 41.463 82 48 0 30 111 73 154 2.27e-14 71.2
Msa0739080 MtrunA17_Chr8g0360031 25.654 191 122 6 72 259 2 175 6.33e-13 67.0
Msa0739080 MtrunA17_Chr2g0317731 26.846 149 99 2 107 245 9 157 6.52e-14 69.7
Msa0739080 MtrunA17_Chr2g0317731 34.653 101 66 0 11 111 51 151 2.58e-13 67.8
Msa0739080 MtrunA17_Chr2g0326241 28.814 118 81 2 17 132 29 145 9.17e-14 68.9
Msa0739080 MtrunA17_Chr2g0326241 28.333 120 83 2 156 273 37 155 3.87e-12 64.3
Msa0739080 MtrunA17_Chr1g0211871 34.483 116 72 2 24 138 64 176 1.16e-13 69.3
Msa0739080 MtrunA17_Chr1g0211871 30.631 111 73 1 153 263 63 169 5.77e-13 67.0
Msa0739080 MtrunA17_Chr1g0201781 32.558 86 58 0 34 119 66 151 5.04e-13 67.0
Msa0739080 MtrunA17_Chr1g0201781 29.808 104 66 3 166 268 68 165 2.00e-11 62.4
Msa0739080 MtrunA17_Chr4g0029181 48.421 95 40 5 30 120 102 191 1.18e-12 69.7
Msa0739080 MtrunA17_Chr2g0317721 34.513 113 68 1 5 111 10 122 4.14e-12 63.5
Msa0739080 MtrunA17_Chr3g0125821 35.632 87 56 0 33 119 73 159 7.10e-12 63.9
Msa0739080 MtrunA17_Chr2g0317711 33.628 113 69 1 5 111 10 122 1.29e-11 62.4
Msa0739080 MtrunA17_Chr4g0041801 32.967 91 52 2 160 242 143 232 1.68e-11 65.9
Msa0739080 MtrunA17_Chr4g0044731 36.905 84 50 1 31 111 63 146 4.47e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0739080 AT1G04980.1 72.748 444 111 4 1 435 5 447 0.0 680
Msa0739080 AT2G32920.1 73.709 426 107 3 14 435 16 440 0.0 656
Msa0739080 AT2G47470.2 36.250 240 133 5 33 267 28 252 4.26e-44 155
Msa0739080 AT2G47470.2 54.369 103 42 3 30 128 143 244 1.40e-27 110
Msa0739080 AT2G47470.2 39.370 127 71 3 163 284 28 153 1.14e-19 89.0
Msa0739080 AT2G47470.3 36.250 240 133 5 33 267 28 252 9.32e-44 156
Msa0739080 AT2G47470.3 54.369 103 42 3 30 128 143 244 1.36e-27 112
Msa0739080 AT2G47470.3 39.370 127 71 3 163 284 28 153 2.75e-19 89.0
Msa0739080 AT2G47470.4 36.250 240 133 5 33 267 28 252 1.01e-43 156
Msa0739080 AT2G47470.4 54.369 103 42 3 30 128 143 244 1.59e-27 112
Msa0739080 AT2G47470.4 39.370 127 71 3 163 284 28 153 2.61e-19 89.0
Msa0739080 AT2G47470.1 36.250 240 133 5 33 267 28 252 1.47e-43 156
Msa0739080 AT2G47470.1 54.369 103 42 3 30 128 143 244 1.71e-27 112
Msa0739080 AT2G47470.1 39.370 127 71 3 163 284 28 153 2.99e-19 89.4
Msa0739080 AT3G54960.2 46.154 91 48 1 30 120 101 190 1.07e-18 89.0
Msa0739080 AT3G54960.2 28.182 220 136 6 163 380 104 303 2.74e-15 78.2
Msa0739080 AT3G54960.1 46.154 91 48 1 30 120 101 190 1.49e-18 88.6
Msa0739080 AT3G54960.1 27.854 219 138 6 163 380 104 303 3.67e-15 78.2
Msa0739080 AT3G54960.1 37.363 91 49 2 36 126 445 527 3.37e-11 65.9
Msa0739080 AT5G60640.1 36.493 211 111 10 30 226 105 306 2.31e-17 84.7
Msa0739080 AT5G60640.1 42.424 99 49 4 161 256 106 199 4.38e-15 77.8
Msa0739080 AT5G60640.1 26.733 202 130 5 68 259 351 544 5.30e-12 68.2
Msa0739080 AT5G60640.2 36.493 211 111 10 30 226 105 306 2.95e-17 84.3
Msa0739080 AT5G60640.2 42.424 99 49 4 161 256 106 199 5.86e-15 77.4
Msa0739080 AT5G60640.2 26.633 199 129 5 62 252 346 535 2.64e-11 65.9
Msa0739080 AT5G60640.3 36.493 211 111 10 30 226 105 306 3.32e-17 84.3
Msa0739080 AT5G60640.3 42.424 99 49 4 161 256 106 199 3.86e-15 77.8
Msa0739080 AT5G60640.3 34.737 95 60 1 167 259 386 480 3.88e-11 65.5
Msa0739080 AT1G21750.2 40.367 109 58 4 30 132 32 139 4.05e-17 84.0
Msa0739080 AT1G21750.2 35.849 106 62 3 163 263 35 139 9.72e-15 76.6
Msa0739080 AT1G21750.2 37.113 97 59 1 26 120 372 468 1.10e-12 70.1
Msa0739080 AT1G21750.1 40.367 109 58 4 30 132 32 139 4.15e-17 84.0
Msa0739080 AT1G21750.1 29.518 166 99 5 163 323 35 187 1.04e-14 76.6
Msa0739080 AT1G21750.1 37.113 97 59 1 26 120 372 468 1.01e-12 70.1
Msa0739080 AT1G21750.1 35.577 104 64 2 165 266 381 483 3.00e-11 65.9
Msa0739080 AT1G77510.1 28.421 190 117 6 152 336 23 198 1.44e-16 82.0
Msa0739080 AT1G77510.1 39.450 109 59 4 30 132 31 138 3.16e-16 81.3
Msa0739080 AT1G77510.1 27.165 254 157 11 14 252 227 467 5.55e-11 64.7
Msa0739080 AT1G07960.4 30.000 120 81 2 28 144 25 144 1.64e-14 70.9
Msa0739080 AT1G07960.4 25.439 114 82 2 154 265 21 133 9.40e-14 68.9
Msa0739080 AT1G07960.3 30.000 120 81 2 28 144 25 144 1.64e-14 70.9
Msa0739080 AT1G07960.3 25.439 114 82 2 154 265 21 133 9.40e-14 68.9
Msa0739080 AT1G07960.1 30.000 120 81 2 28 144 25 144 1.64e-14 70.9
Msa0739080 AT1G07960.1 25.439 114 82 2 154 265 21 133 9.40e-14 68.9
Msa0739080 AT1G07960.2 30.000 120 81 2 28 144 25 144 1.64e-14 70.9
Msa0739080 AT1G07960.2 25.439 114 82 2 154 265 21 133 9.40e-14 68.9
Msa0739080 AT1G35620.1 40.426 94 51 3 30 120 34 125 2.18e-14 75.1
Msa0739080 AT1G35620.1 27.966 236 138 10 155 383 29 239 8.68e-14 73.6
Msa0739080 AT1G43560.1 37.209 86 54 0 34 119 64 149 1.45e-13 68.9
Msa0739080 AT1G76760.1 31.429 105 68 1 34 138 69 169 1.92e-13 68.6
Msa0739080 AT4G03520.1 36.082 97 59 2 151 245 74 169 3.51e-12 65.5
Msa0739080 AT1G50320.1 30.000 150 89 4 121 263 30 170 3.76e-12 65.1
Msa0739080 AT3G15360.1 36.667 90 57 0 30 119 88 177 4.43e-12 65.1
Msa0739080 AT4G27080.1 32.000 125 67 4 126 242 118 232 2.31e-11 65.9
Msa0739080 AT4G27080.2 32.000 125 67 4 126 242 170 284 2.70e-11 65.9
Msa0739080 AT1G03680.1 26.923 156 94 4 110 245 8 163 2.94e-11 62.4
Msa0739080 AT3G20560.1 34.000 100 57 2 151 242 134 232 9.68e-11 63.9

Find 147 sgRNAs with CRISPR-Local

Find 309 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCTTCCCTTTCAAATTAT+TGG 0.104762 5_1:+57126487 None:intergenic
TTCAATCAATTGTTCATTAA+TGG 0.149709 5_1:+57124625 None:intergenic
TTCCCAACAATCTTGGTATT+TGG 0.173156 5_1:-57126067 Msa0739080:CDS
TTCCTTCCTTCCCGACATTT+TGG 0.184244 5_1:-57125787 Msa0739080:CDS
CTTAATTCCCTTCAGGAATA+TGG 0.210374 5_1:-57127272 Msa0739080:intron
TACGAGAAGACCTGGTTCTT+TGG 0.276020 5_1:-57124596 Msa0739080:three_prime_UTR
GAAGGCAGCTACTGTATTAA+AGG 0.276220 5_1:-57128230 Msa0739080:CDS
GATAGTATGTAGAATATTAA+AGG 0.283108 5_1:-57124818 Msa0739080:three_prime_UTR
CTCTAGTAACTTCGATGAAT+TGG 0.285509 5_1:-57126715 Msa0739080:CDS
CTTCAGGAATATGGTATTAG+AGG 0.289766 5_1:-57127263 Msa0739080:intron
CTATATAGCTCTGTCACTTC+TGG 0.297987 5_1:+57125947 None:intergenic
CGCAGAATTTGCACTTCAAC+AGG 0.299216 5_1:-57127165 Msa0739080:intron
TCAATCAATTGTTCATTAAT+GGG 0.300305 5_1:+57124626 None:intergenic
GTTTCCTTATAGTTCATCTT+TGG 0.305542 5_1:+57124902 None:intergenic
TTTGAAAGGGAAGGTTAAAC+TGG 0.309941 5_1:-57126480 Msa0739080:CDS
GTAAGGCTCTGACTCCTATA+TGG 0.316417 5_1:-57128255 Msa0739080:CDS
TGCGATTGGTTTAACATCTC+TGG 0.351595 5_1:+57127184 None:intergenic
AATTTATATAGACTCGTAAA+TGG 0.356732 5_1:+57124299 None:intergenic
CTCAAGGTCTGGCTGCTTAC+CGG 0.356762 5_1:+57125282 None:intergenic
GCAAAAGGGGCTTAGATATA+GGG 0.370495 5_1:+57124265 None:intergenic
TAGAGGATTTCCAACTATAA+AGG 0.370686 5_1:-57127246 Msa0739080:CDS
GTGGCTTGAAACTCGTATTA+AGG 0.371667 5_1:-57124482 Msa0739080:three_prime_UTR
AACACCTTTCTTAACGTTCA+AGG 0.373568 5_1:+57125216 None:intergenic
TGCAAAAGGGGCTTAGATAT+AGG 0.373607 5_1:+57124264 None:intergenic
GCTCGAACGTTGCATCATAA+AGG 0.379435 5_1:+57124424 None:intergenic
CTCGAACGTTGCATCATAAA+GGG 0.386422 5_1:+57124425 None:intergenic
TCAACCAAAGATGAACTATA+AGG 0.387123 5_1:-57124906 Msa0739080:exon
AAAGTTGGTGTTGGTGGGTA+TGG 0.390826 5_1:-57125256 Msa0739080:CDS
CAACGTTCGAGCTATGTTTA+CGG 0.396118 5_1:-57124413 Msa0739080:three_prime_UTR
GGGTCTTGAACAGCTATGTA+TGG 0.397598 5_1:-57125312 Msa0739080:intron
CTAGCACCCTCGTAAGGAAT+TGG 0.409040 5_1:+57126028 None:intergenic
CATCTCCTATAGCATAAAAC+TGG 0.409206 5_1:+57124840 None:intergenic
TCAAGGATTCCCAACAATCT+TGG 0.416145 5_1:-57126074 Msa0739080:CDS
TCCATGGTGCGGGCACTGTA+AGG 0.416505 5_1:-57128272 Msa0739080:CDS
ACCTTGGACTTGAAGTTGTT+AGG 0.419712 5_1:+57129708 None:intergenic
TTGTTGAATTCTTTGCTCCA+TGG 0.424265 5_1:-57128288 Msa0739080:CDS
TGCTGATGCTCACCAATCTC+TGG 0.427343 5_1:-57128182 Msa0739080:CDS
TTAGGAGTGAGTTGAAGAAC+GGG 0.430086 5_1:+57129726 None:intergenic
AGTTCAAAAGGAGTCCTTAC+AGG 0.430959 5_1:-57125707 Msa0739080:intron
AGGAATTGGAGTATCTTTAT+CGG 0.435399 5_1:+57126042 None:intergenic
AGAAACCACCGCTTCTTCTT+CGG 0.438238 5_1:-57126748 Msa0739080:CDS
TCTGTTTCTCCCCTCCGCCT+TGG 0.439079 5_1:+57125761 None:intergenic
AGAGAATTTGTGAAAGAAGC+TGG 0.439854 5_1:-57125065 Msa0739080:CDS
GTGTTGTCTATAGGATGTTT+TGG 0.440808 5_1:-57125844 Msa0739080:intron
TTATCAAAAGCAAAGAACTT+TGG 0.443575 5_1:-57126692 Msa0739080:CDS
GGTGCAAACACCTTTATAGT+TGG 0.448420 5_1:+57127236 None:intergenic
CCAACTTTCTGCTCAAGGTC+TGG 0.450805 5_1:+57125271 None:intergenic
TGCTCACCAATCTCTGGCTC+AGG 0.454025 5_1:-57128176 Msa0739080:intron
GTTTAACATCTCTGGCTCCT+TGG 0.454708 5_1:+57127192 None:intergenic
TGTTGAATGTTGCACACAAC+CGG 0.455377 5_1:+57124552 None:intergenic
ATGAGCAGGTTCAATGTTCA+AGG 0.456443 5_1:-57126091 Msa0739080:CDS
AATTCTTTGCTCCATGGTGC+GGG 0.458259 5_1:-57128282 Msa0739080:CDS
ATCATTCGCACTAGAGCAGC+TGG 0.462465 5_1:-57125987 Msa0739080:CDS
TTATAGTTCATCTTTGGTTG+AGG 0.465932 5_1:+57124908 None:intergenic
TGACAGGTTCTGAATTCAAA+TGG 0.467668 5_1:-57128319 Msa0739080:intron
CTCCAAATACCAAGATTGTT+GGG 0.472339 5_1:+57126065 None:intergenic
ATCCTTTCTCATGCTCTCTA+CGG 0.472571 5_1:-57129759 Msa0739080:CDS
TTTGTCAGTCTCTAATGAGC+AGG 0.472766 5_1:-57126105 Msa0739080:intron
GGAAAACCTGAGCCAGAGAT+TGG 0.473756 5_1:+57128170 None:intergenic
CGGTTAAATGGAAAAGCAAC+AGG 0.474554 5_1:-57126789 Msa0739080:CDS
ATAGTATGTAGAATATTAAA+GGG 0.475068 5_1:-57124817 Msa0739080:three_prime_UTR
GCTCCAAATACCAAGATTGT+TGG 0.479181 5_1:+57126064 None:intergenic
ACGTTCAAGGCTACTAATGC+TGG 0.481617 5_1:+57125229 None:intergenic
GTTAGGAGTGAGTTGAAGAA+CGG 0.482632 5_1:+57129725 None:intergenic
AGTGAGTTGAAGAACGGGAG+AGG 0.483997 5_1:+57129731 None:intergenic
CAGTTGCAGAGAAGTTCAAA+AGG 0.484219 5_1:-57125719 Msa0739080:CDS
CTCGTATTAAGGTGAATAAG+TGG 0.484744 5_1:-57124471 Msa0739080:three_prime_UTR
GGTTCTGTCTTTACAATGGT+GGG 0.490337 5_1:+57125002 None:intergenic
ACTATAAAGGTGTTTGCACC+TGG 0.493971 5_1:-57127233 Msa0739080:CDS
GCCTTGGAATCCAAAATGTC+GGG 0.495320 5_1:+57125777 None:intergenic
GAACCATGGGATGGAAAAGA+TGG 0.495841 5_1:-57124984 Msa0739080:CDS
TGGTTCTGTCTTTACAATGG+TGG 0.496152 5_1:+57125001 None:intergenic
CGTTCCTCATTGACCATAGC+CGG 0.496245 5_1:-57124571 Msa0739080:three_prime_UTR
TTAGTGAAGTGTTTAGAAGA+TGG 0.498830 5_1:-57124334 Msa0739080:three_prime_UTR
AGAGCATCCAATAATTTGAA+AGG 0.498898 5_1:-57126494 Msa0739080:CDS
CGCCTTGGAATCCAAAATGT+CGG 0.513014 5_1:+57125776 None:intergenic
AACCGTAGAGAGCATGAGAA+AGG 0.514080 5_1:+57129757 None:intergenic
GAAGCTGGACGTGGAGGCAA+AGG 0.515570 5_1:-57125050 Msa0739080:CDS
AAGGCAGCTACTGTATTAAA+GGG 0.517489 5_1:-57128229 Msa0739080:CDS
GAGCATCCAATAATTTGAAA+GGG 0.518744 5_1:-57126493 Msa0739080:CDS
GTAGCTGCCTTCTCCCATAT+AGG 0.519121 5_1:+57128241 None:intergenic
ATGTTGCACACAACCGGCTA+TGG 0.522020 5_1:+57124558 None:intergenic
TGGTGGGAGGAGTGTCACCT+AGG 0.522614 5_1:+57125018 None:intergenic
ATCCAATAATTTGAAAGGGA+AGG 0.529134 5_1:-57126489 Msa0739080:CDS
TAAGGCTCTGACTCCTATAT+GGG 0.533987 5_1:-57128254 Msa0739080:CDS
CCAGACCTTGAGCAGAAAGT+TGG 0.535182 5_1:-57125271 Msa0739080:CDS
GGTGGGAGGAGTGTCACCTA+GGG 0.535529 5_1:+57125019 None:intergenic
CTGGCTCCTTGGTAATCGAC+TGG 0.539378 5_1:+57127203 None:intergenic
GAATTCTTTGCTCCATGGTG+CGG 0.548779 5_1:-57128283 Msa0739080:CDS
GAGCAGAAAGTTGGTGTTGG+TGG 0.549595 5_1:-57125262 Msa0739080:CDS
TCCTAACAACTTCAAGTCCA+AGG 0.552476 5_1:-57129709 Msa0739080:intron
ATCCAAAATGTCGGGAAGGA+AGG 0.552840 5_1:+57125785 None:intergenic
CCATGGTTCTGTCTTTACAA+TGG 0.553296 5_1:+57124998 None:intergenic
GTAGCCTTGAACGTTAAGAA+AGG 0.556407 5_1:-57125220 Msa0739080:CDS
AACATACCTCAGGAAAATGT+TGG 0.556667 5_1:-57124670 Msa0739080:three_prime_UTR
AATCTACGCTTCTTCAACAA+TGG 0.558002 5_1:-57129829 Msa0739080:exon
CTCTAGTGCGAATGATTCGA+TGG 0.558140 5_1:+57125994 None:intergenic
GGTCTTGAACAGCTATGTAT+GGG 0.559621 5_1:-57125311 Msa0739080:intron
AAAAGATGGAGAAATAATTG+AGG 0.562273 5_1:-57124970 Msa0739080:CDS
TGAAGTGCAAATTCTGCGAT+TGG 0.562719 5_1:+57127170 None:intergenic
GCCTTACAGTGCCCGCACCA+TGG 0.567839 5_1:+57128271 None:intergenic
AACATTATTACGAGAAGACC+TGG 0.569546 5_1:-57124604 Msa0739080:three_prime_UTR
GTTCTTGTATGAAGAGAAGA+AGG 0.570808 5_1:+57129883 None:intergenic
CAACTGTTAATAACTGCTGA+AGG 0.571823 5_1:+57125736 None:intergenic
GCAGTTCTAGCACCCTCGTA+AGG 0.576634 5_1:+57126022 None:intergenic
CAACACCAACTTTCTGCTCA+AGG 0.577087 5_1:+57125266 None:intergenic
AGCAGAAAGTTGGTGTTGGT+GGG 0.581032 5_1:-57125261 Msa0739080:CDS
AAGCTGGACGTGGAGGCAAA+GGG 0.583780 5_1:-57125049 Msa0739080:CDS
TTTGTGAAAGAAGCTGGACG+TGG 0.587104 5_1:-57125059 Msa0739080:CDS
AGCTATGTATGGGCAGCTGC+CGG 0.590014 5_1:-57125301 Msa0739080:CDS
TTCATGTCATTCTCATTCAG+TGG 0.591892 5_1:-57124696 Msa0739080:three_prime_UTR
GGCTTAGATATAGGGACGGA+GGG 0.591898 5_1:+57124273 None:intergenic
AGATTGATGAATGAATCTGC+GGG 0.594548 5_1:+57129847 None:intergenic
TCTGACTCCTATATGGGAGA+AGG 0.598675 5_1:-57128248 Msa0739080:CDS
TCTGTCTTTACAATGGTGGG+AGG 0.605063 5_1:+57125005 None:intergenic
TCGTATTAAGGTGAATAAGT+GGG 0.608145 5_1:-57124470 Msa0739080:three_prime_UTR
GCTCCTTGGTAATCGACTGG+TGG 0.611053 5_1:+57127206 None:intergenic
TAGTTCTACCGAAGAAGAAG+CGG 0.621191 5_1:+57126740 None:intergenic
AGTTCAAAAGCACTCTTGAG+AGG 0.621890 5_1:+57125187 None:intergenic
TCAGTGGTACAACATACCTC+AGG 0.622601 5_1:-57124680 Msa0739080:three_prime_UTR
CATTGTAAAGACAGAACCAT+GGG 0.623172 5_1:-57124997 Msa0739080:CDS
TTCTACCGAAGAAGAAGCGG+TGG 0.624355 5_1:+57126743 None:intergenic
GGCAAAGGGAACTTGCCCCT+AGG 0.627464 5_1:-57125035 Msa0739080:CDS
CTTGAGCAGAAAGTTGGTGT+TGG 0.628583 5_1:-57125265 Msa0739080:CDS
GAGGAACGATCCAAAGAACC+AGG 0.629610 5_1:+57124586 None:intergenic
TTGAAAGGGAAGGTTAAACT+GGG 0.631529 5_1:-57126479 Msa0739080:CDS
AACACTTCACTAATGCATGT+AGG 0.634863 5_1:+57124344 None:intergenic
TGGAATCCAAAATGTCGGGA+AGG 0.635224 5_1:+57125781 None:intergenic
GATACTCCAATTCCTTACGA+GGG 0.636183 5_1:-57126034 Msa0739080:CDS
TGTTGACTGTGATGCTGACA+AGG 0.636371 5_1:-57126453 Msa0739080:intron
GTAAAGACAGAACCATGGGA+TGG 0.640244 5_1:-57124993 Msa0739080:CDS
TATAGCTCTGTCACTTCTGG+AGG 0.640322 5_1:+57125950 None:intergenic
TAGATTGATGAATGAATCTG+CGG 0.646073 5_1:+57129846 None:intergenic
GTGAAAGAAGCTGGACGTGG+AGG 0.650513 5_1:-57125056 Msa0739080:CDS
GGGCTTAGATATAGGGACGG+AGG 0.661411 5_1:+57124272 None:intergenic
TTAAATGGAAAAGCAACAGG+TGG 0.661631 5_1:-57126786 Msa0739080:CDS
AGATACTCCAATTCCTTACG+AGG 0.665654 5_1:-57126035 Msa0739080:CDS
TCGAACGTTGCATCATAAAG+GGG 0.668066 5_1:+57124426 None:intergenic
AAGACGAGTATAGTGATGAG+TGG 0.670606 5_1:-57124732 Msa0739080:three_prime_UTR
GTGGGAGGAGTGTCACCTAG+GGG 0.673404 5_1:+57125020 None:intergenic
GAATATTAAAGGGAAATGTG+TGG 0.677310 5_1:-57124807 Msa0739080:three_prime_UTR
ACAACCGGCTATGGTCAATG+AGG 0.692842 5_1:+57124567 None:intergenic
AAGGGGCTTAGATATAGGGA+CGG 0.695087 5_1:+57124269 None:intergenic
GATTGATGAATGAATCTGCG+GGG 0.699974 5_1:+57129848 None:intergenic
CCATTGTAAAGACAGAACCA+TGG 0.708421 5_1:-57124998 Msa0739080:CDS
ATTGATGAATGAATCTGCGG+GGG 0.723990 5_1:+57129849 None:intergenic
AAACCACCAGTCGATTACCA+AGG 0.727693 5_1:-57127209 Msa0739080:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCATTTTCTTAAATTATTAT+CGG - chr5_1:57124963-57124982 Msa0739080:CDS 10.0%
!!! AAAATTATTCATATTTTCTT+GGG + chr5_1:57125761-57125780 None:intergenic 10.0%
!!! AAAAATTATTCATATTTTCT+TGG + chr5_1:57125762-57125781 None:intergenic 10.0%
!! AATTTAATGACATATATTAA+AGG - chr5_1:57126061-57126080 Msa0739080:CDS 10.0%
!! GTAATAAATAATTGAAATAT+AGG + chr5_1:57127538-57127557 None:intergenic 10.0%
!! TATAAATAAATTGAAAAGTA+AGG + chr5_1:57127975-57127994 None:intergenic 10.0%
!! AATAATATTAAAAGACTGTT+TGG + chr5_1:57125707-57125726 None:intergenic 15.0%
!!! AAATTATTCATATTTTCTTG+GGG + chr5_1:57125760-57125779 None:intergenic 15.0%
!!! AATTTTAAATATTGAATCTG+AGG + chr5_1:57126045-57126064 None:intergenic 15.0%
!! TTATAGCTTATTTAAAAAAC+AGG - chr5_1:57126371-57126390 Msa0739080:intron 15.0%
!!! ATATTTAAAATAGTTACAAG+AGG + chr5_1:57126495-57126514 None:intergenic 15.0%
!!! TTGTAACTATTTTAAATATG+TGG - chr5_1:57126496-57126515 Msa0739080:CDS 15.0%
!!! TAAAATTGTTTGATGTTTTA+AGG + chr5_1:57126561-57126580 None:intergenic 15.0%
!! AATATTTGATTGAAACTTTA+GGG + chr5_1:57127070-57127089 None:intergenic 15.0%
!! AAATATTTGATTGAAACTTT+AGG + chr5_1:57127071-57127090 None:intergenic 15.0%
!! TGAAAAATTACTACAAATAA+AGG - chr5_1:57127176-57127195 Msa0739080:CDS 15.0%
!! AATTTGATTAATAACTTGAA+AGG - chr5_1:57127201-57127220 Msa0739080:CDS 15.0%
!! ATTTGATTAATAACTTGAAA+GGG - chr5_1:57127202-57127221 Msa0739080:CDS 15.0%
!! AAAAATAGACTTACATAATT+TGG + chr5_1:57129013-57129032 None:intergenic 15.0%
!! ATAGTATGTAGAATATTAAA+GGG - chr5_1:57129354-57129373 Msa0739080:intron 15.0%
!! TTGAAAAAAAGATTAAACCT+TGG + chr5_1:57124482-57124501 None:intergenic 20.0%
!! AAAAAACAAGCTAAAAAAGT+GGG + chr5_1:57124529-57124548 None:intergenic 20.0%
!!! TTTTAGCTTGTTTTTTCAAA+TGG - chr5_1:57124533-57124552 Msa0739080:three_prime_UTR 20.0%
!!! TTTAGCTTGTTTTTTCAAAT+GGG - chr5_1:57124534-57124553 Msa0739080:three_prime_UTR 20.0%
!! TATCATCTGAAAAAATCAAT+AGG - chr5_1:57124786-57124805 Msa0739080:three_prime_UTR 20.0%
!! TATCACTAAAATTTAGTTGA+AGG - chr5_1:57125105-57125124 Msa0739080:intron 20.0%
!!! AAGTGATTTTATGTTATGAT+AGG - chr5_1:57125130-57125149 Msa0739080:intron 20.0%
!!! CTTCAAGTAGAATATATTTT+GGG - chr5_1:57125227-57125246 Msa0739080:CDS 20.0%
!!! TTCAAGTAGAATATATTTTG+GGG - chr5_1:57125228-57125247 Msa0739080:CDS 20.0%
!!! AGAACTAATTTTTACATGTT+TGG + chr5_1:57125280-57125299 None:intergenic 20.0%
!! TACTAACTAAACTCAAATTA+AGG - chr5_1:57125446-57125465 Msa0739080:intron 20.0%
!! ACACATATTCATATTCATAA+AGG + chr5_1:57126126-57126145 None:intergenic 20.0%
!!! ATTAAGTTGTTTATCCAAAT+AGG - chr5_1:57126460-57126479 Msa0739080:CDS 20.0%
!!! TTAAGTTGTTTATCCAAATA+GGG - chr5_1:57126461-57126480 Msa0739080:CDS 20.0%
!! CGTTAATGTAATATATTCTT+TGG + chr5_1:57126674-57126693 None:intergenic 20.0%
!!! TGTCTTTTTTTATTTCTCAA+AGG - chr5_1:57127618-57127637 Msa0739080:intron 20.0%
!! TTTAACAAACTGTTATTGTT+TGG - chr5_1:57127827-57127846 Msa0739080:intron 20.0%
!! TCAGAATAATATAAACTGAA+AGG + chr5_1:57128031-57128050 None:intergenic 20.0%
!!! TCTTTCAAATTCAACTTTTT+CGG - chr5_1:57128557-57128576 Msa0739080:intron 20.0%
!! CATGTTTAAATCAAGATAAA+AGG - chr5_1:57128613-57128632 Msa0739080:intron 20.0%
!!! TTGCAGTTATTTTTAAATTG+CGG - chr5_1:57128695-57128714 Msa0739080:intron 20.0%
!! TACTTACATGGAAATATAAT+GGG - chr5_1:57128728-57128747 Msa0739080:intron 20.0%
!!! AATGGTTTTTTGTTTGAATT+TGG - chr5_1:57128838-57128857 Msa0739080:intron 20.0%
!!! ATGGTTTTTTGTTTGAATTT+GGG - chr5_1:57128839-57128858 Msa0739080:intron 20.0%
!!! CTAATTTTATGCTAAATAGT+TGG - chr5_1:57129033-57129052 Msa0739080:intron 20.0%
!! GATAGTATGTAGAATATTAA+AGG - chr5_1:57129353-57129372 Msa0739080:intron 20.0%
!!! TTTTTCAGATTTTTCTTGTT+CGG - chr5_1:57129411-57129430 Msa0739080:intron 20.0%
!! TCAATCAATTGTTCATTAAT+GGG + chr5_1:57129548-57129567 None:intergenic 20.0%
!! TTCAATCAATTGTTCATTAA+TGG + chr5_1:57129549-57129568 None:intergenic 20.0%
!!! TTTTTTTTAAAGTAGCATAG+TGG - chr5_1:57129670-57129689 Msa0739080:intron 20.0%
!! AATTTATATAGACTCGTAAA+TGG + chr5_1:57129875-57129894 None:intergenic 20.0%
! GAAAAAACAAGCTAAAAAAG+TGG + chr5_1:57124530-57124549 None:intergenic 25.0%
!! TTTTGTATCTGTTTTGTGTT+TGG - chr5_1:57124577-57124596 Msa0739080:three_prime_UTR 25.0%
!!! TATGGTTGATTTTATGATTC+TGG - chr5_1:57124663-57124682 Msa0739080:three_prime_UTR 25.0%
!!! ATGGTTGATTTTATGATTCT+GGG - chr5_1:57124664-57124683 Msa0739080:three_prime_UTR 25.0%
! AAATATATTCTACTTGAAGC+TGG + chr5_1:57125226-57125245 None:intergenic 25.0%
!! GCTTCAAGTAGAATATATTT+TGG - chr5_1:57125226-57125245 Msa0739080:CDS 25.0%
!!! TCAAGTAGAATATATTTTGG+GGG - chr5_1:57125229-57125248 Msa0739080:CDS 25.0%
!!! TATGTTTGATTTCACTTTTG+AGG + chr5_1:57125327-57125346 None:intergenic 25.0%
!! TATCTAGTTTGTAATACATC+AGG - chr5_1:57125805-57125824 Msa0739080:CDS 25.0%
!! TGTAATACATCAGGTTTTTA+TGG - chr5_1:57125814-57125833 Msa0739080:CDS 25.0%
!! GTAATACATCAGGTTTTTAT+GGG - chr5_1:57125815-57125834 Msa0739080:CDS 25.0%
! TAATTAAGCACTTATCATGT+AGG + chr5_1:57126444-57126463 None:intergenic 25.0%
!! TAGTGTTACAATTTGGATTT+TGG - chr5_1:57127101-57127120 Msa0739080:intron 25.0%
! AGTGAGAATATCAATGTATT+GGG - chr5_1:57127271-57127290 Msa0739080:intron 25.0%
! AATATCAATGTATTGGGATT+AGG - chr5_1:57127277-57127296 Msa0739080:intron 25.0%
! TATAGTTTATTTGCATGTAC+AGG - chr5_1:57127339-57127358 Msa0739080:intron 25.0%
! TTATCAAAAGCAAAGAACTT+TGG - chr5_1:57127479-57127498 Msa0739080:intron 25.0%
!!! TTTTTTATTTCTCAAAGGTG+TGG - chr5_1:57127623-57127642 Msa0739080:intron 25.0%
! TCAGTTTATATTATTCTGAC+AGG - chr5_1:57128032-57128051 Msa0739080:intron 25.0%
! GTACTTACATGGAAATATAA+TGG - chr5_1:57128727-57128746 Msa0739080:intron 25.0%
! AAAAGATGGAGAAATAATTG+AGG - chr5_1:57129201-57129220 Msa0739080:intron 25.0%
TAGATTGATGAATGAATCTG+CGG + chr5_1:57124328-57124347 None:intergenic 30.0%
! TTTTATGATTCTGGGTTTTG+CGG - chr5_1:57124672-57124691 Msa0739080:three_prime_UTR 30.0%
TTCCATGTAAACAAATGTGT+CGG + chr5_1:57124938-57124957 None:intergenic 30.0%
TACACAACATATATGAAGCA+CGG + chr5_1:57125027-57125046 None:intergenic 30.0%
!! TACACCTCTAGAATCAATTT+CGG - chr5_1:57125299-57125318 Msa0739080:CDS 30.0%
ATCATACGTGTGAATATAGA+AGG + chr5_1:57125395-57125414 None:intergenic 30.0%
AACTCAAATTAAGGTCTGTT+TGG - chr5_1:57125455-57125474 Msa0739080:intron 30.0%
AATAAGAGGAAAACACCTTA+TGG + chr5_1:57125563-57125582 None:intergenic 30.0%
GAAGAGCTTATGAAAATAAG+AGG + chr5_1:57125577-57125596 None:intergenic 30.0%
! TATTCATATTTTCTTGGGGA+GGG + chr5_1:57125756-57125775 None:intergenic 30.0%
! TTATTCATATTTTCTTGGGG+AGG + chr5_1:57125757-57125776 None:intergenic 30.0%
CTAGAAATACATACCTTTGA+TGG - chr5_1:57126179-57126198 Msa0739080:intron 30.0%
TCGAATAAACCAATCTAAAC+AGG + chr5_1:57126235-57126254 None:intergenic 30.0%
TATTGACATAAGTACCTGTT+AGG - chr5_1:57126261-57126280 Msa0739080:intron 30.0%
ATTGACATAAGTACCTGTTA+GGG - chr5_1:57126262-57126281 Msa0739080:intron 30.0%
TTTCATAAACTTTCCCTAAC+AGG + chr5_1:57126278-57126297 None:intergenic 30.0%
! TTGGTTGCTTTACATAGAAA+GGG + chr5_1:57126655-57126674 None:intergenic 30.0%
! TTTGGTTGCTTTACATAGAA+AGG + chr5_1:57126656-57126675 None:intergenic 30.0%
CCTGTTATGATTTACATGTT+CGG - chr5_1:57126799-57126818 Msa0739080:CDS 30.0%
TCTAATACCATATTCCTGAA+GGG + chr5_1:57126909-57126928 None:intergenic 30.0%
TAGAGGATTTCCAACTATAA+AGG - chr5_1:57126925-57126944 Msa0739080:intron 30.0%
! TTTGATTGAAACTTTAGGGA+GGG + chr5_1:57127066-57127085 None:intergenic 30.0%
! ATTTGATTGAAACTTTAGGG+AGG + chr5_1:57127067-57127086 None:intergenic 30.0%
AATTGTAACACTACACATGT+TGG + chr5_1:57127094-57127113 None:intergenic 30.0%
ACATGTGTAGTGTTACAATT+TGG - chr5_1:57127094-57127113 Msa0739080:intron 30.0%
! AGATAGATGTCTTCTTTTTG+TGG - chr5_1:57127131-57127150 Msa0739080:intron 30.0%
GAGTGAGAATATCAATGTAT+TGG - chr5_1:57127270-57127289 Msa0739080:intron 30.0%
TTATTTGCATGTACAGGTAA+AGG - chr5_1:57127345-57127364 Msa0739080:intron 30.0%
TAGAATTACATTGTTCAGTC+AGG + chr5_1:57127560-57127579 None:intergenic 30.0%
!!! CAGGAGCTAATTTTTTACAA+TGG + chr5_1:57127649-57127668 None:intergenic 30.0%
! AGAGCATCCAATAATTTGAA+AGG - chr5_1:57127677-57127696 Msa0739080:intron 30.0%
GAGCATCCAATAATTTGAAA+GGG - chr5_1:57127678-57127697 Msa0739080:intron 30.0%
ATCCAATAATTTGAAAGGGA+AGG - chr5_1:57127682-57127701 Msa0739080:intron 30.0%
AACCTTCCCTTTCAAATTAT+TGG + chr5_1:57127687-57127706 None:intergenic 30.0%
ATGCAGATAGCAAAAAATAG+CGG + chr5_1:57127932-57127951 None:intergenic 30.0%
! AGGAATTGGAGTATCTTTAT+CGG + chr5_1:57128132-57128151 None:intergenic 30.0%
TACAATCAAGTAAAGTTTGC+AGG + chr5_1:57128269-57128288 None:intergenic 30.0%
! TAAACATGAGTGTTGTCTAT+AGG - chr5_1:57128318-57128337 Msa0739080:intron 30.0%
!!! TGTTTTTTTACTTGCCTGTA+AGG + chr5_1:57128481-57128500 None:intergenic 30.0%
ATTAACTCATGAGTCATAGA+GGG + chr5_1:57128538-57128557 None:intergenic 30.0%
!! GAAATATAATGGGTTCTCAT+TGG - chr5_1:57128738-57128757 Msa0739080:intron 30.0%
! ATTTTCTCTTGAAGAACTCA+TGG - chr5_1:57129231-57129250 Msa0739080:intron 30.0%
! TTTTCTCTTGAAGAACTCAT+GGG - chr5_1:57129232-57129251 Msa0739080:intron 30.0%
!! CATTACCAGTTTTATGCTAT+AGG - chr5_1:57129326-57129345 Msa0739080:intron 30.0%
GAATATTAAAGGGAAATGTG+TGG - chr5_1:57129364-57129383 Msa0739080:intron 30.0%
TCGTATTAAGGTGAATAAGT+GGG - chr5_1:57129701-57129720 Msa0739080:intron 30.0%
! TTAGTGAAGTGTTTAGAAGA+TGG - chr5_1:57129837-57129856 Msa0739080:five_prime_UTR 30.0%
GTTCTTGTATGAAGAGAAGA+AGG + chr5_1:57124291-57124310 None:intergenic 35.0%
AGATTGATGAATGAATCTGC+GGG + chr5_1:57124327-57124346 None:intergenic 35.0%
AATCTACGCTTCTTCAACAA+TGG - chr5_1:57124342-57124361 Msa0739080:three_prime_UTR 35.0%
! TTTGCTATGTTGCTGTATTG+TGG - chr5_1:57124603-57124622 Msa0739080:three_prime_UTR 35.0%
TGTAGTGTAGTGTGTGAATA+TGG - chr5_1:57124645-57124664 Msa0739080:three_prime_UTR 35.0%
GGCAAAATCAATTCTACTCT+AGG - chr5_1:57125250-57125269 Msa0739080:CDS 35.0%
! ATTCATATTTTCTTGGGGAG+GGG + chr5_1:57125755-57125774 None:intergenic 35.0%
!! GGTGCTATTTGAATAATGAC+AGG - chr5_1:57125836-57125855 Msa0739080:intron 35.0%
!! TGACAGGTTCTGAATTCAAA+TGG - chr5_1:57125852-57125871 Msa0739080:intron 35.0%
TTGTTGAATTCTTTGCTCCA+TGG - chr5_1:57125883-57125902 Msa0739080:intron 35.0%
AAGGCAGCTACTGTATTAAA+GGG - chr5_1:57125942-57125961 Msa0739080:intron 35.0%
AGGAATGAAGAAATGAGCAA+TGG + chr5_1:57126025-57126044 None:intergenic 35.0%
! GGTATTGAACCTGTTTAGAT+TGG - chr5_1:57126223-57126242 Msa0739080:intron 35.0%
AAGTTATCCTAGAGAGCTTA+TGG + chr5_1:57126340-57126359 None:intergenic 35.0%
CATATGAAATATGTCGAGCA+TGG - chr5_1:57126519-57126538 Msa0739080:CDS 35.0%
TTACATAGAAAGGGAAGACT+AGG + chr5_1:57126646-57126665 None:intergenic 35.0%
ACAAATGTCATGTAAGAGAC+AGG + chr5_1:57126730-57126749 None:intergenic 35.0%
CCGAACATGTAAATCATAAC+AGG + chr5_1:57126802-57126821 None:intergenic 35.0%
! GTCTTTTCTTAATTCCCTTC+AGG - chr5_1:57126892-57126911 Msa0739080:intron 35.0%
CTTAATTCCCTTCAGGAATA+TGG - chr5_1:57126899-57126918 Msa0739080:intron 35.0%
CTCTAATACCATATTCCTGA+AGG + chr5_1:57126910-57126929 None:intergenic 35.0%
CTTCAGGAATATGGTATTAG+AGG - chr5_1:57126908-57126927 Msa0739080:intron 35.0%
!! TAAAGGCTCTTTTGAAGGAA+CGG - chr5_1:57127362-57127381 Msa0739080:intron 35.0%
!! CTTTTGAAGGAACGGTTAAA+TGG - chr5_1:57127370-57127389 Msa0739080:intron 35.0%
TTAAATGGAAAAGCAACAGG+TGG - chr5_1:57127385-57127404 Msa0739080:intron 35.0%
CTCTAGTAACTTCGATGAAT+TGG - chr5_1:57127456-57127475 Msa0739080:intron 35.0%
! TTGTAGAATTTTTCGCACCT+TGG - chr5_1:57127503-57127522 Msa0739080:intron 35.0%
!! AATATAGGAAGTACTCACCA+AGG + chr5_1:57127523-57127542 None:intergenic 35.0%
GTAAAAAATTAGCTCCTGAG+TGG - chr5_1:57127651-57127670 Msa0739080:intron 35.0%
TTTGAAAGGGAAGGTTAAAC+TGG - chr5_1:57127691-57127710 Msa0739080:intron 35.0%
TTGAAAGGGAAGGTTAAACT+GGG - chr5_1:57127692-57127711 Msa0739080:intron 35.0%
TTCCCAACAATCTTGGTATT+TGG - chr5_1:57128104-57128123 Msa0739080:intron 35.0%
CTCCAAATACCAAGATTGTT+GGG + chr5_1:57128109-57128128 None:intergenic 35.0%
! GTGTTGTCTATAGGATGTTT+TGG - chr5_1:57128327-57128346 Msa0739080:intron 35.0%
!!! GATGTTTTGGAAGAGAAATG+TGG - chr5_1:57128340-57128359 Msa0739080:intron 35.0%
CAACTGTTAATAACTGCTGA+AGG + chr5_1:57128438-57128457 None:intergenic 35.0%
GATTAACTCATGAGTCATAG+AGG + chr5_1:57128539-57128558 None:intergenic 35.0%
GTTTGATGAAGATGCATTGT+TGG + chr5_1:57128788-57128807 None:intergenic 35.0%
CTTTGTTGACGAATGCTTAA+TGG - chr5_1:57128820-57128839 Msa0739080:intron 35.0%
AACACCTTTCTTAACGTTCA+AGG + chr5_1:57128958-57128977 None:intergenic 35.0%
AGAGAATTTGTGAAAGAAGC+TGG - chr5_1:57129106-57129125 Msa0739080:intron 35.0%
CATTGTAAAGACAGAACCAT+GGG - chr5_1:57129174-57129193 Msa0739080:intron 35.0%
CATCTCCTATAGCATAAAAC+TGG + chr5_1:57129334-57129353 None:intergenic 35.0%
GAAAAATCTGAAAAACAGCG+AGG + chr5_1:57129406-57129425 None:intergenic 35.0%
TTCATGTCATTCTCATTCAG+TGG - chr5_1:57129475-57129494 Msa0739080:intron 35.0%
AACATACCTCAGGAAAATGT+TGG - chr5_1:57129501-57129520 Msa0739080:intron 35.0%
AACATTATTACGAGAAGACC+TGG - chr5_1:57129567-57129586 Msa0739080:intron 35.0%
CTCGTATTAAGGTGAATAAG+TGG - chr5_1:57129700-57129719 Msa0739080:intron 35.0%
AACACTTCACTAATGCATGT+AGG + chr5_1:57129830-57129849 None:intergenic 35.0%
ATTGATGAATGAATCTGCGG+GGG + chr5_1:57124325-57124344 None:intergenic 40.0%
GATTGATGAATGAATCTGCG+GGG + chr5_1:57124326-57124345 None:intergenic 40.0%
ATCCTTTCTCATGCTCTCTA+CGG - chr5_1:57124412-57124431 Msa0739080:three_prime_UTR 40.0%
! TTAGGAGTGAGTTGAAGAAC+GGG + chr5_1:57124448-57124467 None:intergenic 40.0%
! GTTAGGAGTGAGTTGAAGAA+CGG + chr5_1:57124449-57124468 None:intergenic 40.0%
TCCTAACAACTTCAAGTCCA+AGG - chr5_1:57124462-57124481 Msa0739080:three_prime_UTR 40.0%
ACCTTGGACTTGAAGTTGTT+AGG + chr5_1:57124466-57124485 None:intergenic 40.0%
AAAAAGTGGGATCACGAACT+GGG + chr5_1:57124516-57124535 None:intergenic 40.0%
AAAAAAGTGGGATCACGAAC+TGG + chr5_1:57124517-57124536 None:intergenic 40.0%
!! TATGAAGCACTGACACTCTT+AGG - chr5_1:57124865-57124884 Msa0739080:three_prime_UTR 40.0%
TCTTAGGATTAGGCATGTCT+GGG - chr5_1:57124881-57124900 Msa0739080:three_prime_UTR 40.0%
CACCGACACATTTGTTTACA+TGG - chr5_1:57124933-57124952 Msa0739080:CDS 40.0%
GTTGTGTATGTTTCGTTCTG+TGG - chr5_1:57125039-57125058 Msa0739080:CDS 40.0%
TTGCTTATGACGTGTCCATA+AGG - chr5_1:57125545-57125564 Msa0739080:intron 40.0%
! TAAGGCTCTGACTCCTATAT+GGG - chr5_1:57125917-57125936 Msa0739080:intron 40.0%
GAAGGCAGCTACTGTATTAA+AGG - chr5_1:57125941-57125960 Msa0739080:intron 40.0%
ACCACACACAATGCAAAACA+TGG - chr5_1:57126202-57126221 Msa0739080:intron 40.0%
! ACCATGTTTTGCATTGTGTG+TGG + chr5_1:57126206-57126225 None:intergenic 40.0%
! GCTATTTCCATAAGCTCTCT+AGG - chr5_1:57126330-57126349 Msa0739080:intron 40.0%
TGAAATATGTCGAGCATGGT+AGG - chr5_1:57126523-57126542 Msa0739080:CDS 40.0%
GAAGACTAGGAATGTAAGGA+AGG + chr5_1:57126633-57126652 None:intergenic 40.0%
AAGGGAAGACTAGGAATGTA+AGG + chr5_1:57126637-57126656 None:intergenic 40.0%
GGTGCAAACACCTTTATAGT+TGG + chr5_1:57126938-57126957 None:intergenic 40.0%
!! ACTATAAAGGTGTTTGCACC+TGG - chr5_1:57126938-57126957 Msa0739080:intron 40.0%
!! TGCGATTGGTTTAACATCTC+TGG + chr5_1:57126990-57127009 None:intergenic 40.0%
TGAAGTGCAAATTCTGCGAT+TGG + chr5_1:57127004-57127023 None:intergenic 40.0%
TTTGCACTTCAACAGGTTTG+TGG - chr5_1:57127013-57127032 Msa0739080:intron 40.0%
AGGTTTGTGGAATCTCTTCT+TGG - chr5_1:57127026-57127045 Msa0739080:intron 40.0%
ACTCTTCTTGCAACATCACT+TGG - chr5_1:57127227-57127246 Msa0739080:CDS 40.0%
! ACAGGTAAAGGCTCTTTTGA+AGG - chr5_1:57127357-57127376 Msa0739080:intron 40.0%
CGGTTAAATGGAAAAGCAAC+AGG - chr5_1:57127382-57127401 Msa0739080:intron 40.0%
! TAGTTCTACCGAAGAAGAAG+CGG + chr5_1:57127434-57127453 None:intergenic 40.0%
ATGCTGACAAGGTAAGCTTA+TGG - chr5_1:57127729-57127748 Msa0739080:intron 40.0%
TGCTATAGTGCTTCTATAGC+TGG + chr5_1:57127888-57127907 None:intergenic 40.0%
TTTGTCAGTCTCTAATGAGC+AGG - chr5_1:57128066-57128085 Msa0739080:intron 40.0%
ATGAGCAGGTTCAATGTTCA+AGG - chr5_1:57128080-57128099 Msa0739080:intron 40.0%
TCAAGGATTCCCAACAATCT+TGG - chr5_1:57128097-57128116 Msa0739080:intron 40.0%
GCTCCAAATACCAAGATTGT+TGG + chr5_1:57128110-57128129 None:intergenic 40.0%
AGATACTCCAATTCCTTACG+AGG - chr5_1:57128136-57128155 Msa0739080:intron 40.0%
GATACTCCAATTCCTTACGA+GGG - chr5_1:57128137-57128156 Msa0739080:intron 40.0%
! CTATATAGCTCTGTCACTTC+TGG + chr5_1:57128227-57128246 None:intergenic 40.0%
CAGTTGCAGAGAAGTTCAAA+AGG - chr5_1:57128452-57128471 Msa0739080:intron 40.0%
AGTTCAAAAGGAGTCCTTAC+AGG - chr5_1:57128464-57128483 Msa0739080:intron 40.0%
GGTTGCAAAGTGTACTTACA+TGG - chr5_1:57128716-57128735 Msa0739080:intron 40.0%
GATGCATTGTTGGTGCAAAA+TGG + chr5_1:57128778-57128797 None:intergenic 40.0%
GGTCTTGAACAGCTATGTAT+GGG - chr5_1:57128860-57128879 Msa0739080:intron 40.0%
! GTAGCCTTGAACGTTAAGAA+AGG - chr5_1:57128951-57128970 Msa0739080:intron 40.0%
!! AGTTCAAAAGCACTCTTGAG+AGG + chr5_1:57128987-57129006 None:intergenic 40.0%
GGTTCTGTCTTTACAATGGT+GGG + chr5_1:57129172-57129191 None:intergenic 40.0%
! TGGTTCTGTCTTTACAATGG+TGG + chr5_1:57129173-57129192 None:intergenic 40.0%
! CCATGGTTCTGTCTTTACAA+TGG + chr5_1:57129176-57129195 None:intergenic 40.0%
CCATTGTAAAGACAGAACCA+TGG - chr5_1:57129173-57129192 Msa0739080:intron 40.0%
AAGACGAGTATAGTGATGAG+TGG - chr5_1:57129439-57129458 Msa0739080:intron 40.0%
! TAGTGATGAGTGGTCTTTTG+AGG - chr5_1:57129449-57129468 Msa0739080:intron 40.0%
! AACATGCCAACATTTTCCTG+AGG + chr5_1:57129510-57129529 None:intergenic 40.0%
TGTTGAATGTTGCACACAAC+CGG + chr5_1:57129622-57129641 None:intergenic 40.0%
GTGGCTTGAAACTCGTATTA+AGG - chr5_1:57129689-57129708 Msa0739080:intron 40.0%
TCGAACGTTGCATCATAAAG+GGG + chr5_1:57129748-57129767 None:intergenic 40.0%
CTCGAACGTTGCATCATAAA+GGG + chr5_1:57129749-57129768 None:intergenic 40.0%
CAACGTTCGAGCTATGTTTA+CGG - chr5_1:57129758-57129777 Msa0739080:CDS 40.0%
! AACCGTAGAGAGCATGAGAA+AGG + chr5_1:57124417-57124436 None:intergenic 45.0%
! GCACTGACACTCTTAGGATT+AGG - chr5_1:57124871-57124890 Msa0739080:three_prime_UTR 45.0%
CTCTTAGGATTAGGCATGTC+TGG - chr5_1:57124880-57124899 Msa0739080:three_prime_UTR 45.0%
!! CAAATGTGTCGGTGTCATGT+CGG + chr5_1:57124927-57124946 None:intergenic 45.0%
! GGAGCCGAAATTGATTCTAG+AGG + chr5_1:57125306-57125325 None:intergenic 45.0%
GAATTCTTTGCTCCATGGTG+CGG - chr5_1:57125888-57125907 Msa0739080:intron 45.0%
AATTCTTTGCTCCATGGTGC+GGG - chr5_1:57125889-57125908 Msa0739080:intron 45.0%
! GTAAGGCTCTGACTCCTATA+TGG - chr5_1:57125916-57125935 Msa0739080:intron 45.0%
TCTGACTCCTATATGGGAGA+AGG - chr5_1:57125923-57125942 Msa0739080:intron 45.0%
CATTGTGTGTGGTCCATCAA+AGG + chr5_1:57126195-57126214 None:intergenic 45.0%
AGAGGACACTTGACCCTATT+TGG + chr5_1:57126477-57126496 None:intergenic 45.0%
! TAATCGACTGGTGGTTTTCC+AGG + chr5_1:57126959-57126978 None:intergenic 45.0%
AAACCACCAGTCGATTACCA+AGG - chr5_1:57126962-57126981 Msa0739080:intron 45.0%
! GTTTAACATCTCTGGCTCCT+TGG + chr5_1:57126982-57127001 None:intergenic 45.0%
CGCAGAATTTGCACTTCAAC+AGG - chr5_1:57127006-57127025 Msa0739080:intron 45.0%
AGAAACCACCGCTTCTTCTT+CGG - chr5_1:57127423-57127442 Msa0739080:intron 45.0%
! TTGGATGCTCTTTTCCACTC+AGG + chr5_1:57127668-57127687 None:intergenic 45.0%
! TGTTGACTGTGATGCTGACA+AGG - chr5_1:57127718-57127737 Msa0739080:intron 45.0%
! GAAGCACTATAGCACTGTAG+TGG - chr5_1:57127894-57127913 Msa0739080:intron 45.0%
CTCTAGTGCGAATGATTCGA+TGG + chr5_1:57128180-57128199 None:intergenic 45.0%
! TATAGCTCTGTCACTTCTGG+AGG + chr5_1:57128224-57128243 None:intergenic 45.0%
! TTCCTTCCTTCCCGACATTT+TGG - chr5_1:57128384-57128403 Msa0739080:intron 45.0%
ATCCAAAATGTCGGGAAGGA+AGG + chr5_1:57128389-57128408 None:intergenic 45.0%
TGGAATCCAAAATGTCGGGA+AGG + chr5_1:57128393-57128412 None:intergenic 45.0%
! TCCCGACATTTTGGATTCCA+AGG - chr5_1:57128393-57128412 Msa0739080:intron 45.0%
GCCTTGGAATCCAAAATGTC+GGG + chr5_1:57128397-57128416 None:intergenic 45.0%
CGCCTTGGAATCCAAAATGT+CGG + chr5_1:57128398-57128417 None:intergenic 45.0%
! CGACATTTTGGATTCCAAGG+CGG - chr5_1:57128396-57128415 Msa0739080:intron 45.0%
! ATTTTGGATTCCAAGGCGGA+GGG - chr5_1:57128400-57128419 Msa0739080:intron 45.0%
GGGTCTTGAACAGCTATGTA+TGG - chr5_1:57128859-57128878 Msa0739080:intron 45.0%
CAACACCAACTTTCTGCTCA+AGG + chr5_1:57128908-57128927 None:intergenic 45.0%
!! CTTGAGCAGAAAGTTGGTGT+TGG - chr5_1:57128906-57128925 Msa0739080:intron 45.0%
!! AGCAGAAAGTTGGTGTTGGT+GGG - chr5_1:57128910-57128929 Msa0739080:intron 45.0%
!! AAAGTTGGTGTTGGTGGGTA+TGG - chr5_1:57128915-57128934 Msa0739080:intron 45.0%
ACGTTCAAGGCTACTAATGC+TGG + chr5_1:57128945-57128964 None:intergenic 45.0%
TTTGTGAAAGAAGCTGGACG+TGG - chr5_1:57129112-57129131 Msa0739080:intron 45.0%
! TCTGTCTTTACAATGGTGGG+AGG + chr5_1:57129169-57129188 None:intergenic 45.0%
GTAAAGACAGAACCATGGGA+TGG - chr5_1:57129178-57129197 Msa0739080:intron 45.0%
GAACCATGGGATGGAAAAGA+TGG - chr5_1:57129187-57129206 Msa0739080:intron 45.0%
! TCTCCATCTTTTCCATCCCA+TGG + chr5_1:57129193-57129212 None:intergenic 45.0%
TCAGTGGTACAACATACCTC+AGG - chr5_1:57129491-57129510 Msa0739080:intron 45.0%
! TACGAGAAGACCTGGTTCTT+TGG - chr5_1:57129575-57129594 Msa0739080:intron 45.0%
GCTCGAACGTTGCATCATAA+AGG + chr5_1:57129750-57129769 None:intergenic 45.0%
GAATCTGCGGGGGATGAAAA+TGG + chr5_1:57124315-57124334 None:intergenic 50.0%
! AGTGAGTTGAAGAACGGGAG+AGG + chr5_1:57124443-57124462 None:intergenic 50.0%
ACGACATGGACACTGACATG+TGG + chr5_1:57124992-57125011 None:intergenic 50.0%
AGTGTCCATGTCGTCGTATC+AGG - chr5_1:57124998-57125017 Msa0739080:CDS 50.0%
GTAGCTGCCTTCTCCCATAT+AGG + chr5_1:57125933-57125952 None:intergenic 50.0%
TGCTGATGCTCACCAATCTC+TGG - chr5_1:57125989-57126008 Msa0739080:CDS 50.0%
GGAAAACCTGAGCCAGAGAT+TGG + chr5_1:57126004-57126023 None:intergenic 50.0%
TTCTACCGAAGAAGAAGCGG+TGG + chr5_1:57127431-57127450 None:intergenic 50.0%
CTAGCACCCTCGTAAGGAAT+TGG + chr5_1:57128146-57128165 None:intergenic 50.0%
ATCATTCGCACTAGAGCAGC+TGG - chr5_1:57128184-57128203 Msa0739080:CDS 50.0%
GCAGGAGAACAAACACTCAC+TGG + chr5_1:57128251-57128270 None:intergenic 50.0%
! CATTTTGGATTCCAAGGCGG+AGG - chr5_1:57128399-57128418 Msa0739080:intron 50.0%
! TTTTGGATTCCAAGGCGGAG+GGG - chr5_1:57128401-57128420 Msa0739080:intron 50.0%
CCAACTTTCTGCTCAAGGTC+TGG + chr5_1:57128903-57128922 None:intergenic 50.0%
CCAGACCTTGAGCAGAAAGT+TGG - chr5_1:57128900-57128919 Msa0739080:intron 50.0%
!! GAGCAGAAAGTTGGTGTTGG+TGG - chr5_1:57128909-57128928 Msa0739080:intron 50.0%
GAGGAACGATCCAAAGAACC+AGG + chr5_1:57129588-57129607 None:intergenic 50.0%
CGTTCCTCATTGACCATAGC+CGG - chr5_1:57129600-57129619 Msa0739080:intron 50.0%
ACAACCGGCTATGGTCAATG+AGG + chr5_1:57129607-57129626 None:intergenic 50.0%
ATGTTGCACACAACCGGCTA+TGG + chr5_1:57129616-57129635 None:intergenic 50.0%
TGTCGGACACAGACGCATGT+CGG + chr5_1:57124910-57124929 None:intergenic 55.0%
GGACACCTGATACGACGACA+TGG + chr5_1:57125006-57125025 None:intergenic 55.0%
! TGCTCACCAATCTCTGGCTC+AGG - chr5_1:57125995-57126014 Msa0739080:CDS 55.0%
! GCTCCTTGGTAATCGACTGG+TGG + chr5_1:57126968-57126987 None:intergenic 55.0%
! CTGGCTCCTTGGTAATCGAC+TGG + chr5_1:57126971-57126990 None:intergenic 55.0%
! GCAGTTCTAGCACCCTCGTA+AGG + chr5_1:57128152-57128171 None:intergenic 55.0%
AGCTATGTATGGGCAGCTGC+CGG - chr5_1:57128870-57128889 Msa0739080:intron 55.0%
CTCAAGGTCTGGCTGCTTAC+CGG + chr5_1:57128892-57128911 None:intergenic 55.0%
GTGAAAGAAGCTGGACGTGG+AGG - chr5_1:57129115-57129134 Msa0739080:intron 55.0%
AAGCTGGACGTGGAGGCAAA+GGG - chr5_1:57129122-57129141 Msa0739080:intron 55.0%
! TCCATGGTGCGGGCACTGTA+AGG - chr5_1:57125899-57125918 Msa0739080:intron 60.0%
! TCTGTTTCTCCCCTCCGCCT+TGG + chr5_1:57128413-57128432 None:intergenic 60.0%
GAAGCTGGACGTGGAGGCAA+AGG - chr5_1:57129121-57129140 Msa0739080:intron 60.0%
GGCAAAGGGAACTTGCCCCT+AGG - chr5_1:57129136-57129155 Msa0739080:intron 60.0%
!! GTGGGAGGAGTGTCACCTAG+GGG + chr5_1:57129154-57129173 None:intergenic 60.0%
!! GGTGGGAGGAGTGTCACCTA+GGG + chr5_1:57129155-57129174 None:intergenic 60.0%
!! TGGTGGGAGGAGTGTCACCT+AGG + chr5_1:57129156-57129175 None:intergenic 60.0%
GCCTTACAGTGCCCGCACCA+TGG + chr5_1:57125903-57125922 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 57124275 57129918 57124275 ID=Msa0739080;Name=Msa0739080
chr5_1 mRNA 57124275 57129918 57124275 ID=Msa0739080-mRNA-1;Parent=Msa0739080;Name=Msa0739080-mRNA-1;_AED=0.12;_eAED=0.12;_QI=86|1|1|1|1|1|9|633|435
chr5_1 exon 57124275 57125085 57124275 ID=Msa0739080-mRNA-1:exon:16189;Parent=Msa0739080-mRNA-1
chr5_1 exon 57125175 57125321 57125175 ID=Msa0739080-mRNA-1:exon:16188;Parent=Msa0739080-mRNA-1
chr5_1 exon 57125708 57125853 57125708 ID=Msa0739080-mRNA-1:exon:16187;Parent=Msa0739080-mRNA-1
chr5_1 exon 57125943 57126119 57125943 ID=Msa0739080-mRNA-1:exon:16186;Parent=Msa0739080-mRNA-1
chr5_1 exon 57126454 57126553 57126454 ID=Msa0739080-mRNA-1:exon:16185;Parent=Msa0739080-mRNA-1
chr5_1 exon 57126669 57126832 57126669 ID=Msa0739080-mRNA-1:exon:16184;Parent=Msa0739080-mRNA-1
chr5_1 exon 57127166 57127279 57127166 ID=Msa0739080-mRNA-1:exon:16183;Parent=Msa0739080-mRNA-1
chr5_1 exon 57128177 57128335 57128177 ID=Msa0739080-mRNA-1:exon:16182;Parent=Msa0739080-mRNA-1
chr5_1 exon 57129710 57129918 57129710 ID=Msa0739080-mRNA-1:exon:16181;Parent=Msa0739080-mRNA-1
chr5_1 five_prime_UTR 57129833 57129918 57129833 ID=Msa0739080-mRNA-1:five_prime_utr;Parent=Msa0739080-mRNA-1
chr5_1 CDS 57129710 57129832 57129710 ID=Msa0739080-mRNA-1:cds;Parent=Msa0739080-mRNA-1
chr5_1 CDS 57128177 57128335 57128177 ID=Msa0739080-mRNA-1:cds;Parent=Msa0739080-mRNA-1
chr5_1 CDS 57127166 57127279 57127166 ID=Msa0739080-mRNA-1:cds;Parent=Msa0739080-mRNA-1
chr5_1 CDS 57126669 57126832 57126669 ID=Msa0739080-mRNA-1:cds;Parent=Msa0739080-mRNA-1
chr5_1 CDS 57126454 57126553 57126454 ID=Msa0739080-mRNA-1:cds;Parent=Msa0739080-mRNA-1
chr5_1 CDS 57125943 57126119 57125943 ID=Msa0739080-mRNA-1:cds;Parent=Msa0739080-mRNA-1
chr5_1 CDS 57125708 57125853 57125708 ID=Msa0739080-mRNA-1:cds;Parent=Msa0739080-mRNA-1
chr5_1 CDS 57125175 57125321 57125175 ID=Msa0739080-mRNA-1:cds;Parent=Msa0739080-mRNA-1
chr5_1 CDS 57124908 57125085 57124908 ID=Msa0739080-mRNA-1:cds;Parent=Msa0739080-mRNA-1
chr5_1 three_prime_UTR 57124275 57124907 57124275 ID=Msa0739080-mRNA-1:three_prime_utr;Parent=Msa0739080-mRNA-1
Gene Sequence

>Msa0739080

ATGGCGAAACTCTCATTCATTGCTGTAACTATCTTTCTCTTCAACAATCTCATCCTTTCTCATGCTCTCTACGGTTCTTCCTCTCCCGTTCTTCAACTCACTCCTAACAACTTCAAGTCCAAGGTTCTGAATTCAAATGGAGTTGTTCTTGTTGAATTCTTTGCTCCATGGTGCGGGCACTGTAAGGCTCTGACTCCTATATGGGAGAAGGCAGCTACTGTATTAAAGGGTGTAGTCACTGTAGCAGCACTTGATGCTGATGCTCACCAATCTCTGGCTCAGGAATATGGTATTAGAGGATTTCCAACTATAAAGGTGTTTGCACCTGGAAAACCACCAGTCGATTACCAAGGAGCCAGAGATGTTAAACCAATCGCAGAATTTGCACTTCAACAGGTAAAGGCTCTTTTGAAGGAACGGTTAAATGGAAAAGCAACAGGTGGATCAAATGAAAAAAAAGAAACCACCGCTTCTTCTTCGGTAGAACTAAACTCTAGTAACTTCGATGAATTGGTTATCAAAAGCAAAGAACTTTGGATTGTAGAATTTTTCGCACCTTGGTGTGGCCATTGTAAAAAATTAGCTCCTGAGTGGAAAAGAGCATCCAATAATTTGAAAGGGAAGGTTAAACTGGGTCATGTTGACTGTGATGCTGACAAGTCTCTAATGAGCAGGTTCAATGTTCAAGGATTCCCAACAATCTTGGTATTTGGAGCCGATAAAGATACTCCAATTCCTTACGAGGGTGCTAGAACTGCATCAGCCATCGAATCATTCGCACTAGAGCAGCTGGAAACAAATGTTGCTCCTCCAGAAGTGACAGAGCTATATAGTCCAGATGTTTTGGAAGAGAAATGTGGTTCTGCTGCTATCTGTTTTGTTTCCTTCCTTCCCGACATTTTGGATTCCAAGGCGGAGGGGAGAAACAGATACCTTCAGCAGTTATTAACAGTTGCAGAGAAGTTCAAAAGGAGTCCTTACAGCTATGTATGGGCAGCTGCCGGTAAGCAGCCAGACCTTGAGCAGAAAGTTGGTGTTGGTGGGTATGGTTATCCAGCATTAGTAGCCTTGAACGTTAAGAAAGGTGTTTATGCTCCTCTCAAGAGTGCTTTTGAACTTGACCAAATTATAGAATTTGTGAAAGAAGCTGGACGTGGAGGCAAAGGGAACTTGCCCCTAGGTGACACTCCTCCCACCATTGTAAAGACAGAACCATGGGATGGAAAAGATGGAGAAATAATTGAGGAAGATGAATTTTCTCTTGAAGAACTCATGGGAGAAGATGCCTCAACCAAAGATGAACTATAA
Protein sequence

>Msa0739080

MAKLSFIAVTIFLFNNLILSHALYGSSSPVLQLTPNNFKSKVLNSNGVVLVEFFAPWCGHCKALTPIWEKAATVLKGVVTVAALDADAHQSLAQEYGIRGFPTIKVFAPGKPPVDYQGARDVKPIAEFALQQVKALLKERLNGKATGGSNEKKETTASSSVELNSSNFDELVIKSKELWIVEFFAPWCGHCKKLAPEWKRASNNLKGKVKLGHVDCDADKSLMSRFNVQGFPTILVFGADKDTPIPYEGARTASAIESFALEQLETNVAPPEVTELYSPDVLEEKCGSAAICFVSFLPDILDSKAEGRNRYLQQLLTVAEKFKRSPYSYVWAAAGKQPDLEQKVGVGGYGYPALVALNVKKGVYAPLKSAFELDQIIEFVKEAGRGGKGNLPLGDTPPTIVKTEPWDGKDGEIIEEDEFSLEELMGEDASTKDEL*