AlfalfaGEDB | Msa0866250

M. sativa cultivar zhongmu-4 / Msa0866250

KEGG: K08360
GO: GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0055114
PFAM: PF03188.19

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0866250	XP_003614405.1	97.510	241	6	0	2	242	4	244	1.92e-167	472

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0866250	sp\|Q9C540\|ACFR4_ARATH	63.968	247	73	3	1	242	1	236	8.67e-97	285

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0866250	G7JX23	97.510	241	6	0	2	242	4	244	9.16e-168	472

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0027820	Msa0866250	0.803354	3.629281e-49	-8.615850e-47
Msa0174450	Msa0866250	0.801200	1.007397e-48	-8.615850e-47
Msa0378720	Msa0866250	0.813740	2.196324e-51	-8.615850e-47
Msa0474730	Msa0866250	0.805032	1.623606e-49	-8.615850e-47
Msa0626450	Msa0866250	0.818299	2.107695e-52	-8.615850e-47
Msa0626490	Msa0866250	0.815297	9.934472e-52	-8.615850e-47
Msa0664600	Msa0866250	0.813862	2.065080e-51	-8.615850e-47
Msa0729100	Msa0866250	0.831529	1.585434e-55	-8.615850e-47
Msa0730430	Msa0866250	0.803578	3.260133e-49	-8.615850e-47
Msa0766930	Msa0866250	0.818737	1.677126e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0866250	MtrunA17_Chr5g0422371	97.510	241	6	0	2	242	4	244	1.76e-171	472
Msa0866250	MtrunA17_Chr6g0456901	44.934	227	120	3	1	226	1	223	2.29e-60	190
Msa0866250	MtrunA17_Chr1g0203371	41.071	224	123	5	1	222	1	217	2.50e-47	156
Msa0866250	MtrunA17_Chr1g0161921	39.151	212	117	4	15	224	16	217	1.82e-42	143
Msa0866250	MtrunA17_Chr1g0161941	33.333	207	129	5	14	219	9	207	1.41e-31	115
Msa0866250	MtrunA17_Chr8g0375991	35.122	205	120	6	14	216	9	202	5.90e-28	105

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0866250	AT1G26100.1	63.968	247	73	3	1	242	1	236	8.82e-98	285
Msa0866250	AT5G38630.1	46.222	225	109	6	10	228	10	228	2.78e-62	195
Msa0866250	AT4G25570.1	39.437	213	123	4	9	220	8	215	2.71e-44	149
Msa0866250	AT4G25570.2	39.437	213	123	4	9	220	49	256	1.31e-43	149
Msa0866250	AT1G14730.1	30.698	215	137	4	14	226	20	224	2.04e-23	94.7

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Find 73 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAGCAAAGGGAGTAGTG+AGG	0.647530	5_4:-67709646	None:intergenic
AAATGCGAACAGTGAGGATA+AGG	0.435065	5_4:+67712440	Msa0866250:CDS
AAGAGCAAAGGGAGTAGTGA+GGG	0.491250	5_4:-67709645	None:intergenic
AAGGGGTGGCTTTAGCTTCT+GGG	0.303519	5_4:+67710126	Msa0866250:CDS
AATCCAATGACCATGAGCAC+AGG	0.602677	5_4:-67709887	None:intergenic
ACAAAATTCCAAGTAAATGA+TGG	0.437217	5_4:+67710164	Msa0866250:CDS
ACATTTAGTTACAGTTGTTA+TGG	0.357021	5_4:+67709611	Msa0866250:exon
ACTACTAACTAGTACACTCT+TGG	0.484995	5_4:-67709546	None:intergenic
ACTCTCGCAAGAAAGAGCAA+AGG	0.634673	5_4:-67709657	None:intergenic
AGATGGTTGCCTGGGTCAAG+GGG	0.483244	5_4:+67710074	Msa0866250:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGGGAATGAAGAAAATAG+AGG	-	chr5_4:67709801-67709820	None:intergenic	30.0%
ATTTCTATAGCCTTCATTCA+TGG	+	chr5_4:67710198-67710217	Msa0866250:CDS	30.0%
AACTAGCTAAATTGGAAAGT+AGG	-	chr5_4:67710614-67710633	None:intergenic	30.0%
CGAAAAGTTGACATGTTTAT+AGG	-	chr5_4:67710773-67710792	None:intergenic	30.0%
AGATTCTATGAGAGTTAACT+TGG	-	chr5_4:67711582-67711601	None:intergenic	30.0%
ATGCATAATATATGCAAGGT+TGG	+	chr5_4:67712184-67712203	Msa0866250:intron	30.0%
TTCAACATATTGTATTGCAG+TGG	+	chr5_4:67712383-67712402	Msa0866250:intron	30.0%
TCCATGGTTGTAAATTCTTT+AGG	+	chr5_4:67712592-67712611	Msa0866250:CDS	30.0%
GTTGTAAATTCTTTAGGCTT+AGG	+	chr5_4:67712598-67712617	Msa0866250:CDS	30.0%
CCTTGAAGAATTTGATACTT+GGG	-	chr5_4:67712667-67712686	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_4	gene	67709534	67712830	67709534	ID=Msa0866250;Name=Msa0866250
chr5_4	mRNA	67709534	67712830	67709534	ID=Msa0866250-mRNA-1;Parent=Msa0866250;Name=Msa0866250-mRNA-1;_AED=0.11;_eAED=0.11;_QI=96\|1\|1\|1\|1\|1\|4\|104\|242
chr5_4	exon	67709534	67709785	67709534	ID=Msa0866250-mRNA-1:exon:21409;Parent=Msa0866250-mRNA-1
chr5_4	exon	67709878	67709929	67709878	ID=Msa0866250-mRNA-1:exon:21410;Parent=Msa0866250-mRNA-1
chr5_4	exon	67710060	67710256	67710060	ID=Msa0866250-mRNA-1:exon:21411;Parent=Msa0866250-mRNA-1
chr5_4	exon	67712403	67712830	67712403	ID=Msa0866250-mRNA-1:exon:21412;Parent=Msa0866250-mRNA-1
chr5_4	five_prime_UTR	67709534	67709629	67709534	ID=Msa0866250-mRNA-1:five_prime_utr;Parent=Msa0866250-mRNA-1
chr5_4	CDS	67709630	67709785	67709630	ID=Msa0866250-mRNA-1:cds;Parent=Msa0866250-mRNA-1
chr5_4	CDS	67709878	67709929	67709878	ID=Msa0866250-mRNA-1:cds;Parent=Msa0866250-mRNA-1
chr5_4	CDS	67710060	67710256	67710060	ID=Msa0866250-mRNA-1:cds;Parent=Msa0866250-mRNA-1
chr5_4	CDS	67712403	67712726	67712403	ID=Msa0866250-mRNA-1:cds;Parent=Msa0866250-mRNA-1
chr5_4	three_prime_UTR	67712727	67712830	67712727	ID=Msa0866250-mRNA-1:three_prime_utr;Parent=Msa0866250-mRNA-1

Gene Sequence

>Msa0866250

ATGGCCACTGTCTTTCCCTCACTACTCCCTTTGCTCTTTCTTGCGAGAGTTTGTGGTCTTTCAGTAGCATTTCTTGTTCTCTTCTGGGCTCTGGCTTTCAAATCTAGCTTCCTCAATACTCCTAATTCTCTTCCTCAACAGGATCTCATCTATGCAGTTCTCCATCCTGTGCTCATGGTCATTGGATTTATCCTTTTAAGTGGAGAAGCAATTTTGGTGCATAGATGGTTGCCTGGGTCAAGGGGGTTGAAGAAATCGGTGCATTTATGTCTTCAAGGGGTGGCTTTAGCTTCTGGGATATTTGGGATTTGGACAAAATTCCAAGTAAATGATGGGATTGTGGCTAATTTCTATAGCCTTCATTCATGGATGGGTTTGATTTGTGTATCACTCTTTGGAGCTCAGTGGCTAATAGGCTTCTTGAACTTTTGGCACCGAGGAGAAATGCGAACAGTGAGGATAAGGATCTTACCATGGCATGTATTTCTTGGCCTATACACTTATGCATTGGCAGTAGCAACGGCTGAAACTGGATTATTAGAGAAGTTGACATTCTTGCAAACACAGAGAAATGTATCCAAACACAGCACTGAGTCCATGGTTGTAAATTCTTTAGGCTTAGGTCTTGCTCTCCTTAGTGGCTTTGTTATTCTGGCTGCTGTTTCACCCAAGTATCAAATTCTTCAAGGCAAACTTTTGTACTCAGATTCTAGGTGTTTGTCATCTTAA

Protein sequence

>Msa0866250

MATVFPSLLPLLFLARVCGLSVAFLVLFWALAFKSSFLNTPNSLPQQDLIYAVLHPVLMVIGFILLSGEAILVHRWLPGSRGLKKSVHLCLQGVALASGIFGIWTKFQVNDGIVANFYSLHSWMGLICVSLFGAQWLIGFLNFWHRGEMRTVRIRILPWHVFLGLYTYALAVATAETGLLEKLTFLQTQRNVSKHSTESMVVNSLGLGLALLSGFVILAAVSPKYQILQGKLLYSDSRCLSS*